Magnetic suction heating device and biological detection equipment

Abstract

The utility model relates to the technical field of high-throughput sequencing, in particular to a magnetic suction heating device and biological detection equipment, the magnetic suction heating device comprises a heat conduction part, the upper surface of the heat conduction part is provided with a plurality of heating positions, the plurality of heating positions are used for placing reaction containers, and mounting gaps are formed between at least part of the heating positions; the heat source piece is in heat-conducting connection with the heat-conducting piece and heats the reaction container through the heat-conducting piece; the magnetic attraction part is arranged in the mounting gap and is used for attracting the magnetic beads in the reaction container; and the shell covers the outer side of the heat conduction piece, the shell is provided with an opening, and the plurality of heating positions are exposed from the opening. The magnetic suction heating device provided by the utility model is beneficial to improving the hybridization efficiency of a sequencing library.

Description

Technical Field

[0001] This application relates to the field of high-throughput sequencing technology, and in particular to a magnetic heating device and a biological detection device. Background Technology

[0002] With the development of next-generation sequencing (NGS) technology, its applications in early cancer screening, companion diagnostics, and infectious diseases are becoming increasingly widespread. NGS technology can perform high-throughput, high-depth genome analysis in a short time, enabling early disease detection, precise diagnosis, and treatment. NGS technology includes library amplification and purification, probe hybridization, capture, elution, and library quality control. Among these, the probe hybridization and probe capture processes are crucial to the accuracy and reliability of experimental results. The probe hybridization process requires controlled reaction temperature. Probe capture requires cleaning magnetic beads to remove impurities and restore activity.

[0003] Existing NGS technology uses a heating module to heat the reaction vessel to complete the probe hybridization process, and a magnetic module to magnetically attract the reaction vessel to complete the probe capture process.

[0004] However, after the reaction vessel is transferred from the heating module to the magnetic module, the temperature in the reaction vessel drops rapidly, which inhibits the hybridization reaction of the sequencing library and thus reduces the hybridization efficiency of the sequencing library. Utility Model Content

[0005] The magnetic heating device and biodetection equipment provided in this application help improve the hybridization efficiency of sequencing libraries.

[0006] In a first aspect, this application provides a magnetic heating device for a biological detection device, comprising: a heat-conducting component, the upper surface of which is provided with a plurality of heating positions for placing a reaction vessel, and at least some of the heating positions are provided with an installation gap; a heat source component, which is thermally connected to the heat-conducting component and heats the reaction vessel through the heat-conducting component; a magnetic suction component, which is disposed in the installation gap and is used to attract magnetic beads inside the reaction vessel; and a housing, which covers the outside of the heat-conducting component and has an opening through which the plurality of heating positions are exposed.

[0007] In one possible implementation, the heat-conducting element includes: a base portion, to which the housing is connected; and a heat-conducting portion disposed on the upper side of the base portion and extending from the opening, wherein the heating positions are all formed on the upper surface of the heat-conducting portion.

[0008] In one possible implementation, the heat-conducting portion comprises a plurality of heat conductors spaced apart along a first direction, each heat conductor having a plurality of heating positions distributed along a second direction; and an installation gap extending along the second direction is defined between two adjacent heat conductors.

[0009] In one possible implementation, the height of the magnetic chuck is higher than the height of the mounting gap.

[0010] In one possible implementation, the housing is provided with a plurality of fixing structures located around the periphery of the opening, and the fixing structures are used to fix the magnetic component.

[0011] In one possible implementation, the fixing structure is located on both sides of the opening along the second direction, the fixing structure is formed as a pressing cantilever, the fixing structure is located on the upper side of the installation gap, and presses against the upper surface of the magnetic member.

[0012] In one possible implementation, within the mounting gap, the magnetic attractor is adapted to move between a first position and a second position, wherein in the first position, the magnetic beads in the reaction vessel are attracted by the magnetic force of the magnetic attractor, and in the second position, the magnetic beads in the reaction vessel are disengaged from the magnetic force of the magnetic attractor.

[0013] In one possible implementation, the magnetic attractor is an electromagnetic component, so that the magnetic attraction force of the magnetic attractor on the magnetic beads in the reaction vessel can be controlled by energizing and de-energizing the magnetic attractor.

[0014] In one possible implementation, the housing covers the upper surface of the base portion and at least a portion of the side surfaces of the base portion.

[0015] In one possible implementation, the base portion includes: a first base portion; a second base portion disposed above the first base portion, wherein the projection of the second base portion in the reference plane is located inside the projection of the first base portion in the reference plane, so that a first step structure is formed between the first base portion and the second base portion, and the projection of the heat-conducting portion in the reference plane is located inside the projection of the second base portion in the reference plane, so that a second step structure is formed between the heat-conducting portion and the second base portion; the housing has a first support structure and a second support structure, wherein the first support structure is supported on the first step structure, and the second support structure is supported on the second step structure.

[0016] In one possible implementation, the second base portion is provided with a plurality of spaced-apart perforated structures, and the heat-conducting portion is located at the connection of the perforated structures.

[0017] In one possible implementation, the heat source is located on the side of the heat-conducting element opposite to the heating position.

[0018] In one possible implementation, the heat source includes: a heating element; a temperature detection unit connected to the heating element to detect the temperature of the heating element; and a temperature control unit connected to both the heating element and the temperature detection unit, wherein the temperature control unit is used to regulate the temperature of the heating element.

[0019] In one possible implementation, the heating element is at least one of a semiconductor heating and cooling chip, an electric heating element, a ceramic heater, an electric heating film, and an electric heating wire.

[0020] In one possible implementation, the housing is made of thermal insulation material.

[0021] Secondly, this application provides a biological detection device, including: a magnetic heating device as described in any of the above possible implementations.

[0022] The magnetic heating device and biodetection equipment provided in this application achieve heating of the reaction container by setting multiple heating positions on the upper surface of a heat-conducting component for placing the reaction container, and by setting a heat source component that can be thermally connected to the heat-conducting component on one side of the heat-conducting component. By setting installation gaps between some of the heating positions and setting magnetic components at the installation gaps, the sequencing library in the reaction container can be magnetically attracted. By setting magnetic components at the installation gaps and covering the outside of the heat-conducting component with an opening to expose the heating positions, the heating and magnetic attraction functions can be integrated into a single design. This avoids the problem of rapid temperature drop when the reaction container is transferred from the heating module to the magnetic attraction module in the prior art, effectively ensuring that the hybridization reaction of the sequencing library can proceed normally and improving the hybridization efficiency of the sequencing library. Attached Figure Description

[0023] The accompanying drawings, which are incorporated in and form part of this specification, illustrate embodiments consistent with this application and, together with the description, serve to explain the principles of this application.

[0024] Figure 1 Schematic diagram of the magnetic heating device provided in this application Figure 1 ;

[0025] Figure 2 Schematic diagram of the magnetic heating device provided in this application Figure 2 .

[0026] Explanation of reference numerals in the attached figures:

[0027] 1-Magnetic heating device;

[0028] 10 - Thermal conductive components;

[0029] 110 - Heating position;

[0030] 120 - Base portion; 121 - First base portion; 122 - Second base portion; 1221 - Hollowed-out structure; 123 - First step structure; 124 - Second step structure;

[0031] 130 - Thermal conductive part; 131 - Thermal conductor;

[0032] 140 - Installation gap;

[0033] 20 - Heat source components;

[0034] 30 - Magnetic suction element;

[0035] 40 - Casing;

[0036] 410 - Opening; 420 - Cantilever; 430 - First support structure; 440 - Second support structure;

[0037] 2-Reaction vessel.

[0038] The accompanying drawings illustrate specific embodiments of this application, which will be described in more detail below. These drawings and descriptions are not intended to limit the scope of the concept in any way, but rather to illustrate the concept of this application to those skilled in the art through reference to particular embodiments. Detailed Implementation

[0039] Exemplary embodiments will now be described in detail, examples of which are illustrated in the accompanying drawings. When the following description relates to the drawings, unless otherwise indicated, the same numbers in different drawings denote the same or similar elements. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with this application. Rather, they are merely examples of apparatuses and methods consistent with some aspects of this application as detailed in the appended claims.

[0040] As illustrated in the background section, existing NGS technologies use a heating module to heat the reaction vessel to complete the probe hybridization process. A magnetic module then magnetically attracts the reaction vessel to complete the probe capture process. However, after the reaction vessel is transferred from the heating module to the magnetic module, the temperature inside the reaction vessel drops rapidly, inhibiting the hybridization reaction of the sequencing library and thus reducing the hybridization efficiency of the sequencing library.

[0041] In view of this, this application provides a magnetic heating device and a biodetection apparatus, which integrates magnetic attraction and heating functions into one unit, solving the problem caused by the separation of the heating module and magnetic attraction module in the prior art. By setting multiple heating positions on the upper surface of the heat-conducting component, the reaction container can be stably placed and uniformly heated. By setting a heat source component, a suitable temperature environment can be provided for key processes such as probe hybridization. At the same time, the magnetic attraction component is set in the installation gap between the heating positions, which can efficiently attract magnetic beads in the reaction container. With the optimization and movement control of the magnetic attraction component position, such as flexible adjustment between the first and second positions, it is ensured that magnetic attraction operation can be performed as needed during the heating process to meet the experimental needs at different stages. In addition, by setting a shell on the outside of the heat-conducting component, the stability and compactness of the entire magnetic heating device structure can be ensured. Overall, this device optimizes the biodetection process and improves the hybridization efficiency of sequencing libraries.

[0042] It is important to understand that NGS technology can sequence hundreds of thousands to millions of DNA molecules at once, obtaining a large amount of gene sequence information in a short time. This greatly improves the efficiency and throughput of sequencing, making large-scale genome sequencing projects feasible. Furthermore, it significantly reduces costs compared to traditional sequencing technologies, thus finding wide application in many fields such as tumor research, genetic disease diagnosis, and microbial detection.

[0043] Key steps in NGS technology include library amplification and purification, probe hybridization, capture, elution, and library quality control. Sequencing library construction involves extracting genomic DNA or RNA from samples (such as blood or tissue), fragmenting it into short fragments of appropriate length, and then ligating specific adapter sequences to both ends of these fragments. These adapter sequences serve two purposes: they are used for subsequent amplification operations, and they facilitate recognition and binding by sequencing instruments, thus achieving successful sequencing library construction. The probe hybridization process utilizes the principle of complementary base pairing, allowing labeled (e.g., fluorescently labeled) nucleic acid probes to specifically bind to target nucleic acid fragments in the library. Probes are generally artificially designed and synthesized, and can be short nucleic acid chains complementary to the target gene region or specific sequence. Under suitable hybridization buffer, temperature, and pH conditions, the probe can hybridize with the target fragment to form a double-stranded structure. By controlling hybridization conditions (especially temperature—too high a temperature can cause the probe to dissociate from the target DNA, while too low a temperature can easily lead to non-specific binding) the specificity and efficiency of hybridization are ensured, laying the foundation for subsequent accurate capture of the target fragment.

[0044] After hybridization, the target fragment bound to the probe needs to be selected from the complex library mixture; this process is called capture. For example, by using magnetic beads to couple probes, after the probe hybridizes with the target fragment, the magnetic beads, with their magnetic attraction properties, can be collected under the influence of an external magnetic field, thus enriching the target fragment. This allows for subsequent detection of the target fragment, improving the specificity and efficiency of sequencing, reducing unnecessary large-scale whole-genome sequencing, and saving costs and time.

[0045] The following is for reference. Figure 1 , Figure 2 The magnetic heating device 1 provided in this application embodiment includes a heat-conducting component 10, a heat source component 20, a magnetic component 30, and a housing 40.

[0046] The upper surface of the heat-conducting component 10 may be provided with multiple heating positions 110. These multiple heating positions 110 are used to place the reaction vessel 2. Optionally, the upper surface of the heat-conducting component 10 may have 96 heating positions 110, which may correspond to the 96 reaction positions in the reaction vessel 2, or to the 96 reaction vessels 2. The reaction vessel 2 may be a PCR tube. The heating position 110 may be a cylindrical groove with a diameter slightly larger than the outer diameter of the PCR tube, allowing the PCR tube to be easily placed inside while ensuring sufficient contact between the bottom of the PCR tube and the heating position 110, facilitating heat conduction. Furthermore, it also ensures temperature uniformity within the reaction vessel 2. Further, at least some of the heating positions 110 are provided with installation gaps 140. The installation gaps 140 are used to install the magnetic suction component 30. Alternatively, all heating positions 110 may be provided with installation gaps 140 to accommodate the spatial arrangement of the magnetic suction component 30 under extreme position conditions.

[0047] The heat source 20 is the heat source of the magnetic heating device 1. The heat source 20 can be thermally connected to the heat-conducting element 10, and by transferring the heat it generates to the heat-conducting element 10, it can provide heat to the container 2. Depending on the different biological detection experimental requirements, the heat source 20 can generate heat of different intensities and durations to meet different temperature control conditions such as heating and constant temperature, creating a suitable temperature environment for the smooth progress of the reaction.

[0048] The magnetic attractor 30 can be disposed in the installation gap 140. The magnetic attractor 30 is used to attract magnetic beads inside the reaction vessel 2. The magnetic attractor 30 can be rod-shaped, sheet-shaped, or block-shaped so that it can be easily inserted into the installation gap 140.

[0049] The housing 40 can cover the outside of the heat-conducting component 10 to protect the internal components such as the heat-conducting component 10, the heat source component 20, and the magnetic suction component 30. The housing 40 has an opening 410 to expose multiple heating positions 110. This allows the reaction vessel 2 to be easily placed into and removed from the heating positions 110, while also keeping the heating positions 110 in a fixed position within the relatively enclosed housing 40, ensuring good contact between the reaction vessel 2 and the heat-conducting component 10, which is beneficial for heat conduction.

[0050] It is understood that the magnetic heating device 1 provided in this application embodiment can heat the reaction container 2 by providing multiple heating positions 110 for placing the reaction container 2 on the upper surface of the heat-conducting element 10, and by providing a heat source element 20 that can be thermally connected to the heat-conducting element 10 on one side of the heat-conducting element 10. By providing an installation gap 140 between some of the heating positions 110 and providing a magnetic suction element 30 at the installation gap 140, the sequencing library in the reaction container 2 can be magnetically suctioned. By providing a magnetic suction element 30 at the installation gap 140 and covering the outside of the heat-conducting element 10 with an opening 410 to expose the heating positions 110, the heating and magnetic suction functions are integrated, achieving synchronous heating and magnetic suction. This avoids the problem of rapid temperature drop caused by the reaction container 2 being transferred from the heating module to the magnetic suction module in the prior art, effectively ensuring that the hybridization reaction of the sequencing library can proceed normally and improving the hybridization efficiency of the sequencing library.

[0051] In one possible implementation, refer to Figure 1 The heat-conducting component 10 includes a base portion 120 and a heat-conducting portion 130. The base portion 120 can be connected to the housing 40 to make the structure of the magnetic heating device 1 more stable. The housing 40 can be engaged or pressed with the base portion 120. The heat-conducting portion 130 can be disposed on the upper side of the base portion 120. Furthermore, the heat-conducting portion 130 can extend from the opening 410 of the housing 40. Further, a plurality of heating positions 110 can be formed on the upper surface of the heat-conducting portion 130.

[0052] Understandably, the heat-conducting part 130 is located on the upper side of the base part 120 and extends from the opening 410 of the shell 40, so that the multiple heating positions 110 formed on the upper surface of the heat-conducting part 130 can provide multiple operable positions for the reaction vessel 2, realizing the high-throughput experimental requirements. At the same time, this design not only makes the magnetic heating device 1 compact, but also facilitates operation by the operator, who can easily place the reaction vessel 2 on the heating position 110 for subsequent heating and magnetic attraction operations.

[0053] In one possible implementation, refer to Figure 1The heat-conducting part 130 can be divided into multiple heat-conducting bodies 131. These multiple heat-conducting bodies 131 can be spaced apart along a first direction. Further, each heat-conducting body 131 is provided with a plurality of heating positions 110. Specifically, the plurality of heating positions 110 on each heat-conducting body 131 can be distributed along a second direction. Furthermore, a mounting gap 140 for mounting the magnetic suction member 30 can be defined between two adjacent heat-conducting bodies 131. The mounting gap 140 can extend along the second direction. Optionally, there can be 12 heat-conducting bodies 131. Each heat-conducting body 131 can be provided with 8 heating positions 110.

[0054] It should be understood that the first direction can refer to the X direction, or it can also refer to the width direction of the housing 40. The second direction can refer to the Y direction, or it can also refer to the extension direction of the magnetic member 30. In addition, a third direction can also be included. The third direction can refer to the Z direction.

[0055] In one possible implementation, refer to Figure 2 The height of the magnetic attractor 30 is higher than the height of the installation gap 140. This allows the magnetic attractor 30 to fully utilize its ability to attract magnetic beads within the reaction vessel 2, enabling its magnetic field to better cover and penetrate the reaction vessel 2. The design of the magnetic attractor 30 being higher than the installation gap 140 ensures that the magnetic field generated by the magnetic attractor 30 is not weakened by the spatial limitations of the installation gap 140, thus acting more effectively on the magnetic beads within the reaction vessel 2, enhancing the attraction force on the magnetic beads, and ensuring that the magnetic beads can be attracted and manipulated more efficiently.

[0056] In one possible implementation, the housing 40 is provided with multiple fixing structures. These fixing structures can secure the magnetic attractor 30. The fixing structures can be located around the periphery of the opening 410. Optionally, the fixing structure can be an elastic clamping structure. The elastic clamping structure can be made of elastic metal or plastic. The elastic clamping structure can have openable and closable clamping arms. By pressing the clamping arms to open them, and after the magnetic attractor 30 is inserted, the clamping arms clamp the magnetic attractor 30 using their own elastic restoring force, fixing it in the mounting gap 140. Alternatively, the fixing structure can be a snap-fit ​​structure. The snap-fit ​​structure can include a snap hole located around the periphery of the opening 410 and a snap head located on the magnetic attractor 30, or vice versa. When the magnetic attractor 30 is inserted into the mounting gap 140, the magnetic attractor 30 is secured by snapping the snap head into the snap hole. The snap hole and snap head can be circular, square, or other shapes to ensure a tight fit.

[0057] In one possible implementation, refer to Figure 1The fixing structure can be a pressure cantilever 420. The fixing structure can be located on both sides of the opening 410 along the second direction. Furthermore, the fixing structure can be positioned above the mounting gap 140. When the housing 40 covers the heat-conducting component 10, the fixing structure can press against the upper surface of the magnetic component 30. The fixing structure can be made of a rigid material, such as hard metal (e.g., aluminum alloy) or high-strength engineering plastic (e.g., polyoxymethylene), to ensure it maintains a stable shape and sufficient strength when pressing against the magnetic component 30.

[0058] Optionally, the pressure cantilever 420 may include a fixed connection portion, a main body portion, and a pressure contact portion. The fixed connection portion of the pressure cantilever 420 can be securely connected to the edge of the opening 410 of the housing 40. The connection method can be threaded connection, welding, or integral molding, etc. For example, a threaded hole is provided on the edge of the opening 410 of the housing 40, and the fixed connection portion is provided with a corresponding threaded post, and the fixing is achieved by tightening the thread. The main body portion can extend upward from the fixed connection portion, and its length can be determined according to the relative position of the housing 40 and the magnetic suction member 30 to ensure that when the housing 40 is covered on the heat-conducting member 10, the pressure contact portion can press against the upper surface of the magnetic suction member 30. A layer of wear-resistant material (such as a hard alloy coating or ceramic coating) can be provided on the plane of the pressure contact portion that contacts the magnetic suction member 30 to enhance wear resistance and extend the service life of the pressure cantilever 420.

[0059] In one possible implementation, within the mounting gap 140, the magnetic attractor 30 can move between a first position and a second position to meet the magnetic attraction requirements of the reaction vessel 2 at different times. Specifically, in the first position, the magnetic beads inside the reaction vessel 2 are subject to the magnetic attraction force of the magnetic attractor 30. In the second position, the magnetic beads inside the reaction vessel 2 can disengage from the magnetic attraction force of the magnetic attractor 30. To enable the movement of the magnetic attractor 30, a linear guide rail can be provided within the mounting gap 140, along which the magnetic attractor 30 can move. The magnetic attractor 30 can also be provided with a slider or guide groove that matches the guide rail, allowing the magnetic attractor 30 to move between the first and second positions along the guide rail. Specifically, a stepper motor can be used to control the position of the magnetic attractor 30.

[0060] Understandably, the first position can refer to a location near the bottom or side of reaction vessel 2. When the magnetic suction element 30 is in the first position, it can generate a magnetic attraction force on the magnetic beads inside reaction vessel 2, thus adsorbing the magnetic beads at the bottom of reaction vessel 2. This facilitates the separation of the magnetic beads from the solution for subsequent extraction, washing, and other operations. In experimental stages such as magnetic bead capture and separation, the magnetic suction element 30 can be in the first position. For example, during nucleic acid extraction, after the magnetic beads bind to the target nucleic acid molecules, the magnetic suction element 30 can be moved to the first position, causing the magnetic beads to adhere to the bottom of reaction vessel 2, so that the supernatant can be removed, the magnetic beads can be washed, and the target nucleic acid molecules can be enriched.

[0061] The second position can refer to the position where the magnetic attractor 30 is away from the bottom or side of the reaction vessel 2. When the magnetic attractor 30 is in the second position, the magnetic force on the magnetic beads is negligible, allowing the magnetic beads to move freely in the solution, which is beneficial for the magnetic beads to mix thoroughly with other components in the solution or to undergo chemical reactions. The magnetic attractor 30 can be in the second position during stages where the magnetic beads need to be mixed or react with other substances. For example, when using magnetic beads for an immunoassay, the magnetic beads and sample can be mixed in the solution first. In this case, the magnetic attractor 30 is in the second position, allowing the magnetic beads to be evenly distributed in the solution, ensuring that the antibodies or antigens on the magnetic beads fully bind to the corresponding components in the sample.

[0062] In one possible implementation, the magnetic attractor 30 can also be an electromagnetic component. By energizing and de-energizing the magnetic attractor 30, flexible control over the adsorption and release of magnetic beads within the reaction vessel 2 can be achieved. Thus, by configuring the magnetic attractor 30 as an electromagnetic component, the magnetic beads can be adsorbed when needed and released when not needed by controlling the on / off state of the control circuit, making the operation of the magnetic beads more flexible and precise. In experiments such as nucleic acid extraction and immunomagnetic bead detection, it is possible to easily separate and mix magnetic beads with other substances, avoiding experimental errors caused by excessively strong or weak magnetic bead adsorption.

[0063] In one possible implementation, refer to Figure 1 , Figure 2 The housing 40 can be installed over the upper surface of the base portion 120 and at least part of its side surfaces. Alternatively, the housing 40 can be installed over the upper surface of the base portion 120 and its side surfaces. This installation method of the housing 40 can enclose critical areas of the base portion 120, preventing the base portion 120 and its supported components such as the heat-conducting component 10 and magnetic component 30 from being exposed to the external environment, effectively preventing damage to the internal components of the housing 40 caused by external physical impacts or operational errors. Simultaneously, this installation method can also constrain the heat generated by the heat source component 20 inside the housing 40 to a certain extent, preventing heat dissipation into the surrounding environment and affecting other components.

[0064] In one possible implementation, refer to Figure 1 , Figure 2 The base portion 120 includes a first base portion 121 and a second base portion 122. The second base portion 122 may be disposed above the first base portion 121. Furthermore, the projection of the second base portion 122 into the reference plane is located inside the projection of the first base portion 121 into the reference plane. That is, the second base portion 122 is smaller in size than the first base portion 121 and is located inside the first base portion 121. Thus, a first step structure 123 can be formed between the first base portion 121 and the second base portion 122. Further, the projection of the heat-conducting portion 130 into the reference plane may be located inside the projection of the second base portion 122 into the reference plane. That is, the heat-conducting portion 130 is smaller in size than the second base portion 122 and is located inside the second base portion 122. Thus, a second step structure 124 can be formed between the heat-conducting portion 130 and the second base portion 122.

[0065] It should be understood that the reference surface can be a plane parallel to the operating surface of the magnetic heating device 1. For example, when the magnetic heating device 1 is placed on a horizontal worktable, the reference surface can be a horizontal plane. In this case, the reference surface can be perpendicular to a third direction.

[0066] To connect the housing 40 to the heat-conducting component 10, the housing 40 may be provided with a first support structure 430 and a second support structure 440. Specifically, the first support structure 430 corresponds to the first step structure 123; the second support structure 440 corresponds to the second step structure 124. The first support structure 430 is supported by the first step structure 123; the second support structure 440 is supported by the second step structure 124.

[0067] It is understandable that the first step structure 123 and the second step structure 124 provide a supporting foundation for the shell 40. That is, the first support structure 430 and the second support structure 440 are respectively supported by the first step structure 123 and the second step structure 124, which enhances the structural stability of the entire magnetic heating device 1. This structural support method ensures that the relative position of the shell and the heat-conducting component 10 remains stable during operation, such as during heating and magnetic attraction, avoiding misalignment or deformation due to operational vibration or other external forces. In addition, the above design allows for the layered utilization of space, improving the compactness of the magnetic heating device 1 and avoiding spatial conflicts between components.

[0068] In one possible implementation, refer to Figure 1 The second base portion 122 is provided with a plurality of hollow structures 1221. The plurality of hollow structures 1221 are distributed at intervals. Furthermore, the heat-conducting portion 130 may be located at the connection of the hollow structures 1221.

[0069] Understandably, the multiple spaced perforated structures 1221 effectively reduce the weight of the second base portion 122, making the entire magnetic heating device 1 lighter. The perforated structures 1221 also guide heat transfer; when the heat source 20 generates heat, the heat can diffuse through the connections between the perforated structures 1221, and ultimately be transferred to the reaction vessel 2 via the heat-conducting part 130, resulting in higher heat conduction efficiency and improved heating efficiency of the reaction vessel. Furthermore, the heat-conducting part 130 is positioned at the connections of the perforated structures 1221, allowing for a tighter connection between the heat-conducting part 130 and the second base portion 122. Positioning the heat-conducting part 130 at the connections of the perforated structures 1221 utilizes the reinforcing portions surrounding the perforated structures 1221, preventing deformation or displacement of the heat-conducting part 130 when bearing the weight of the reaction vessel 2 and external forces during operation, thus ensuring the stability and reliability of the heating process.

[0070] In one possible implementation, refer to Figure 1 , Figure 2 The heat source component 20 is located on one side of the heat-conducting component 10. Specifically, the heat source component 20 can be located on the side of the heat-conducting component 10 away from the heating position 110.

[0071] In one possible implementation, the heat source 20 includes a heating element, a temperature detection unit, and a temperature control unit. The temperature detection unit can be connected to the heating element to detect its temperature. The temperature control unit can be connected to both the heating element and the temperature detection unit to regulate the temperature of the heating element.

[0072] In one possible implementation, the heating element is at least one of a semiconductor heating and cooling chip, an electric heating element, a ceramic heater, an electric heating film, and an electric heating wire. For example, the heating element can be one of these components. Alternatively, the heating element can be a combination of two or more of these components. For example, the heating element can be a combination of a ceramic heater and an electric heating wire. Alternatively, the heating element can be a combination of a semiconductor heating and cooling chip, an electric heating film, and a ceramic heater. The specific arrangement of the heating element can be determined according to actual needs, and this application does not impose any limitations.

[0073] In one possible implementation, the housing 40 is made of heat-insulating material. This allows for greater temperature stability within the reaction vessel 2. Furthermore, the heat-insulating housing 40 prevents the surface temperature of the magnetic heating device 1 from becoming excessively high, reducing the risk of burns to operators due to accidental contact and providing a safer operating environment.

[0074] Furthermore, this application provides a biological detection device, including the aforementioned magnetic heating device 1. It should be noted that the magnetic heating device 1 provided in this embodiment can be applied to various types of biological detection devices, such as high-throughput sequencer-related equipment, gene chip detection equipment, and nucleic acid molecular diagnostic equipment.

[0075] It is understood that the biodetection device of this application, by integrating the aforementioned magnetic heating device 1, can improve the efficiency and reliability of biodetection. The magnetic heating device 1, by integrating heating and magnetic attraction functions, solves the problem of rapid temperature drop caused by transferring the reaction vessel 2 between different modules in the prior art, ensuring the continuity and efficiency of the sequencing library hybridization reaction.

[0076] In the description of the embodiments of this application, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, an indirect connection through an intermediate medium, or the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in the embodiments of this application according to the specific circumstances.

[0077] The devices or elements referred to in the embodiments of this application or implied herein must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as limiting the embodiments of this application. In the description of the embodiments of this application, "a plurality of" means two or more, unless otherwise precisely specified.

[0078] The terms "first," "second," "third," "fourth," etc. (if present) in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate so that the embodiments of this application described herein can be implemented, for example, in orders other than those illustrated or described herein.

[0079] Furthermore, the terms “comprising” and “having”, and any variations thereof, are intended to cover non-exclusive inclusion, such that a process, method, system, product, or apparatus that includes a series of steps or units is not necessarily limited to those steps or units that are explicitly listed, but may include other steps or units that are not explicitly listed or that are inherent to such process, method, product, or apparatus.

[0080] The term "multiple" in this article refers to two or more. The term "and / or" in this article is merely a description of the relationship between related objects, indicating that there can be three relationships. For example, A and / or B can represent three cases: A exists alone, A and B exist simultaneously, and B exists alone.

[0081] It is understood that the various numerical designations used in the embodiments of this application are merely for descriptive convenience and are not intended to limit the scope of the embodiments of this application.

[0082] It is understood that, in the embodiments of this application, the order of the above-mentioned process numbers does not imply the order of execution. The execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of this application.

[0083] Other embodiments of this application will readily occur to those skilled in the art upon consideration of the specification and practice of the utility models disclosed herein. This application is intended to cover any variations, uses, or adaptations of this application that follow the general principles of this application and include common knowledge or customary techniques in the art not disclosed herein. The specification and examples are to be considered exemplary only, and the true scope and spirit of this application are indicated by the following claims.

[0084] It should be understood that this application is not limited to the precise structure described above and shown in the accompanying drawings, and various modifications and changes can be made without departing from its scope. The scope of this application is limited only by the appended claims.

Claims

1. A magnetic heating device for use in biological detection equipment, characterized in that, include: A heat-conducting component, wherein the upper surface of the heat-conducting component is provided with a plurality of heating positions, the plurality of heating positions being used to place a reaction vessel, and at least some of the heating positions are provided with an installation gap; A heat source component is thermally connected to the heat-conducting component and heats the reaction vessel through the heat-conducting component; A magnetic suction element is provided in the installation gap, and the magnetic suction element is used to attract magnetic beads inside the reaction vessel; A housing is provided on the outside of the heat-conducting element, the housing having an opening through which a plurality of the heating positions are exposed.

2. The magnetic heating device according to claim 1, characterized in that, The heat-conducting component includes: The base portion, to which the shell is connected; A heat-conducting part is provided on the upper side of the base part and extends out from the opening, and the heating positions are all formed on the upper surface of the heat-conducting part.

3. The magnetic heating device according to claim 2, characterized in that, The heat-conducting portion consists of a plurality of heat-conducting bodies spaced apart along a first direction, and each heat-conducting body is provided with a plurality of heating positions distributed along a second direction; The mounting gap is defined between two adjacent heat conductors, extending along the second direction.

4. The magnetic heating device according to claim 3, characterized in that, The height of the magnetic chuck is higher than the height of the mounting gap.

5. The magnetic heating device according to claim 4, characterized in that, The housing is provided with multiple fixing structures, which are located around the opening and are used to fix the magnetic component.

6. The magnetic heating device according to claim 5, characterized in that, The fixing structure is provided on both sides of the opening along the second direction. The fixing structure is formed as a pressure cantilever, and the fixing structure is located on the upper side of the installation gap and is in pressure engagement with the upper surface of the magnetic suction member.

7. The magnetic heating device according to claim 1, characterized in that, Within the installation gap, the magnetic attractor is adapted to move between a first position and a second position, wherein in the first position, the magnetic beads in the reaction vessel are attracted by the magnetic force of the magnetic attractor, and in the second position, the magnetic beads in the reaction vessel are disengaged from the magnetic force of the magnetic attractor.

8. The magnetic heating device according to claim 1, characterized in that, The magnetic attractor is an electromagnetic component, which controls the magnetic attraction force of the magnetic attractor on the magnetic beads in the reaction vessel by energizing and de-energizing the magnetic attractor.

9. The magnetic heating device according to any one of claims 2-8, characterized in that, The housing covers the upper surface of the base portion and at least a portion of the side surfaces of the base portion.

10. The magnetic heating device according to claim 9, characterized in that, The base portion includes: First base section; The second base portion is disposed on the upper side of the first base portion, and the projection of the second base portion in the reference plane is located inside the projection of the first base portion in the reference plane, so that a first step structure is formed between the first base portion and the second base portion, and the projection of the heat-conducting portion in the reference plane is located inside the projection of the second base portion in the reference plane, so that a second step structure is formed between the heat-conducting portion and the second base portion. The shell has a first support structure and a second support structure, the first support structure being supported by the first step structure, and the second support structure being supported by the second step structure.

11. The magnetic heating device according to claim 10, characterized in that, The second base portion has multiple spaced-apart hollow structures, and the heat-conducting portion is located at the connection of the hollow structures.

12. The magnetic heating device according to any one of claims 1-8, characterized in that, The heat source is located on the side of the heat-conducting component opposite to the heating position.

13. The magnetic heating device according to any one of claims 1-8, characterized in that, The heat source component includes: Heating section; A temperature detection unit is connected to the heating element to detect the temperature of the heating element; A temperature control unit is connected to both the heating element and the temperature detection unit, and the temperature control unit is used to regulate the temperature of the heating element.

14. The magnetic heating device according to claim 13, characterized in that, The heating element is at least one of a semiconductor heating and cooling chip, an electric heating element, a ceramic heater, an electric heating film, and an electric heating wire.

15. The magnetic heating device according to any one of claims 1-8, characterized in that, The shell is made of heat-insulating material.

16. A biological detection device, characterized in that, include: The magnetic heating device according to any one of claims 1-15.

Images