Sperm analysis auxiliary device

Abstract

The utility model discloses an auxiliary device for sperm analysis, which comprises a glass slide and a cover glass, the cover glass is arranged right above the middle of the glass slide, the length of the cover glass is smaller than that of the glass slide, the width of the cover glass is smaller than that of the glass slide, and four corners of the lower part of the cover glass are connected with the glass slide through connecting columns. And the distance between the lower end surface of the cover glass and the upper end surface of the glass slide is 5.5-6 microns. The upper end face or the lower end face of the cover glass is provided with longitudinal lines parallel to each other and transverse lines parallel to each other, and the longitudinal lines are perpendicular to the transverse lines. The sperm analysis auxiliary device has the advantages that the sperm analysis auxiliary device is simple in structure and convenient to operate, can automatically and quantitatively detect the sample size of sperm, simplifies operation, avoids manual operation errors, accurately measures various parameter indexes of sperms, and is accurate in sperm analysis and measurement result.

Description

Technical Field

[0001] This utility model relates to the field of sperm analysis technology, specifically to a sperm analysis auxiliary device. Background Technology

[0002] Semen analysis is often used to evaluate male fertility. Currently, semen analysis typically involves placing a certain amount of semen on a glass slide, covering it with a coverslip, and then observing it under a microscope. This method is cumbersome because the slide and coverslip are separate components. The coverslip is thin and smooth, making it prone to falling off and causing contamination. Furthermore, the coverslip's weight makes it difficult to control the semen thickness and ensure its level, resulting in uneven thickness at one end and a thinner end. This difference in semen thickness affects the sperm count per unit area, thus influencing the sperm count and density measurements. In other words, for the same patient, measurements taken more than once may yield inconsistent results due to variations in semen thickness on different slides and coverslips. Additionally, when the semen is thin, the coverslip can compress the sperm, affecting sperm motility and impacting sperm vitality observations. Utility Model Content

[0003] The purpose of this invention is to provide a sperm analysis auxiliary device that is easy to operate and provides accurate sperm analysis results.

[0004] To achieve the above objectives, this utility model provides the following technical solution: a sperm analysis auxiliary device, comprising a glass slide and a coverslip, wherein the coverslip is disposed directly above the center of the glass slide, the length of the coverslip is less than the length of the glass slide, the width of the coverslip is less than the width of the glass slide, the four lower corners of the coverslip are connected to the glass slide via connecting posts, and the distance between the lower end face of the coverslip and the upper end face of the glass slide is 5.5μm-6μm.

[0005] The present invention may further include the following technical solution: the upper or lower end surface of the cover glass is provided with parallel longitudinal lines and parallel transverse lines, wherein the longitudinal lines and the transverse lines are perpendicular to each other.

[0006] The present invention may further include the following technical solution: the distance between adjacent longitudinal lines and adjacent transverse lines is 180-500μm.

[0007] The present invention may further include the following technical solution: a semen storage cavity is provided on the glass slide near one end of the cover glass.

[0008] The semen to be tested is added into the semen storage chamber. The semen above the gap between the slide and the coverslip enters between the slide and the coverslip under the action of siphon and gravity, forming a semen layer of uniform thickness. Excess semen is left in the semen storage chamber to prevent it from flowing out of the slide and causing contamination.

[0009] The present invention may further include the following technical solution: the volume of the semen storage cavity is 100-200 microliters.

[0010] This utility model's sperm analysis auxiliary device allows for easy use; semen is simply dropped onto one end of a coverslip or into the semen storage cavity. Under the influence of siphon and gravity, the semen flows between the coverslip and the slide, forming a uniformly thick semen layer. This ensures accurate measurement of sperm count and concentration without affecting sperm motility, resulting in accurate sperm motility observations. Furthermore, multiple observation windows formed by longitudinal and transverse lines allow for the measurement of sperm count, concentration, motility, and motility per unit area. The device can also calculate the average of the corresponding values ​​from multiple observation windows, leading to more reasonable and accurate results.

[0011] In summary, the advantages of this utility model are as follows: The sperm analysis auxiliary device of this utility model has a simple structure and is easy to operate. By setting the gap height between the slide and the coverslip, the thickness of the semen between the slide and the coverslip can be made uniform, and the sample volume of semen can be automatically and quantitatively detected. At the same time, it can also ensure the free movement of sperm. The tester only needs to add the semen to be tested to one end of the coverslip or the semen storage cavity, which simplifies the operation, avoids human operation error, accurately calculates various parameters of sperm, and the sperm analysis results are accurate. Attached Figure Description

[0012] Figure 1 This is a schematic diagram of the overall structure of Embodiment 1 of the present invention;

[0013] Figure 2 for Figure 1 A top-view structural diagram.

[0014] Figure 3 This is a schematic diagram of the overall structure of Embodiment 2 of this utility model;

[0015] Figure 4 for Figure 3 A top-view structural diagram.

[0016] Attached image labels:

[0017] 1. Glass slide; 2. Coverslip; 3. Longitudinal line; 4. Transverse line; 5. Connecting column; 6. Semen storage chamber. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] Example 1: As Figure 1-2 As shown, this utility model discloses a sperm analysis auxiliary device, including a glass slide 1 and a coverslip 2. The coverslip 2 is positioned directly above the center of the glass slide 1. The length of the coverslip 2 is less than the length of the glass slide 1, and the width of the coverslip 2 is less than the width of the glass slide 1. The four lower corners of the coverslip 2 are connected to the glass slide 1 via connecting posts 5. The distance H between the lower end face of the coverslip 2 and the upper end face of the glass slide 1 is 5.5 μm. Parallel longitudinal lines 3 and parallel transverse lines 4 are arranged on the lower end face of the coverslip 2. The longitudinal lines 3 and transverse lines 4 are perpendicular to each other. The distance between adjacent longitudinal lines and adjacent transverse lines is 240 μm.

[0020] Example 2: Figure 3-4 As shown, this utility model discloses a sperm analysis auxiliary device, including a glass slide 1 and a coverslip 2. The coverslip 2 is positioned directly above the center of the glass slide 1. The length of the coverslip 2 is less than the length of the glass slide 1, and the width of the coverslip 2 is less than the width of the glass slide 1. The four lower corners of the coverslip 2 are connected to the glass slide 1 via connecting posts 5. The distance H between the lower end face of the coverslip 2 and the upper end face of the glass slide 1 is 6 μm. Parallel longitudinal lines 3 and parallel transverse lines 4 are provided on the lower end face of the coverslip 2. The longitudinal lines 3 and transverse lines 4 are perpendicular to each other. The distance between adjacent longitudinal lines and adjacent transverse lines is 360 μm. A semen storage cavity 6 is provided on the glass slide 1 near one end of the coverslip 2. The volume of the semen storage cavity 6 is 120 microliters.

Claims

1. A sperm analysis auxiliary device, comprising a glass slide and a coverslip, characterized in that, The cover glass is positioned directly above the center of the slide. The length of the cover glass is less than the length of the slide, and the width of the cover glass is less than the width of the slide. The four lower corners of the cover glass are connected to the slide via connecting posts. The cover glass has parallel longitudinal lines and parallel transverse lines on its upper or lower surface, and the longitudinal lines and transverse lines are perpendicular to each other. The slide has a semen storage cavity near one end of the cover glass.

2. The sperm analysis auxiliary device according to claim 1, characterized in that, The distance between the lower end face of the cover glass and the upper end face of the slide is 5.5μm-6μm.

3. The sperm analysis auxiliary device according to claim 1 or 2, characterized in that, The distance between adjacent longitudinal lines and adjacent transverse lines is 180-500 μm.

4. The sperm analysis auxiliary device according to claim 1 or 2, characterized in that, The volume of the semen storage chamber is 100-200 microliters.

5. The sperm analysis auxiliary device according to claim 3, characterized in that, The volume of the semen storage chamber is 100-200 microliters.

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