Giant salamander peptide purity detection device

Abstract

The utility model discloses a giant salamander peptide purity detection device, which belongs to the technical field of detection equipment and comprises a crushing cylinder, a liquid injection mechanism connected to the upper side of a crushing cylinder body, a top butt joint port in threaded connection with the lower end of the crushing cylinder body, and a discharge pipe with a control valve at the bottom of the crushing cylinder extends into a filter cylinder in the centrifugal cylinder. A weighing ring mechanism is arranged on the inner bottom surface of the crushing cylinder, a boss in the center of the inner bottom surface of the crushing cylinder is rotationally connected with a rotating shaft of a crushing cutter, the top surface of the rotating shaft of the crushing cutter is connected with a driving mechanism, and all the mechanisms and a control valve are electrically connected to a control screen; according to the device, the fish meat feeding mass is measured through the weighing ring mechanism, a proper amount of reagent is automatically added according to the feeding amount to dissolve peptide in fish meat, the dissolved peptide turbid solution is injected into the filter cylinder to be centrifuged to remove insoluble substances, the prepared peptide solution is filtered and then used for high performance liquid chromatography to analyze the peptide purity, and the device has the advantages of being easy to operate and high in automation degree and has a wide application prospect. The detection efficiency of the giant salamander peptide purity is improved.

Description

Technical Field

[0001] This utility model relates to the field of detection equipment technology, specifically to a device for detecting the purity of peptides in giant salamanders. Background Technology

[0002] The giant salamander, also known as the Chinese giant salamander, is the largest living amphibian in the world. Wild giant salamanders are a Class II protected animal in China, but second-generation and offspring of captive-bred giant salamanders can be used for aquatic product or resource development. Among these, the active peptides in giant salamander meat are a key research focus for major biotechnology companies. These are small-molecule active peptides that can effectively scavenge free radicals, enhance the body's immunity, and promote the proliferation of skin fibroblasts, showing broad application prospects in the food, pharmaceutical, and cosmetic fields. To better develop giant salamander active peptides, accurate and efficient determination of peptide content in the meat is needed. This is usually done using high-performance liquid chromatography (HPLC), which requires preparing a dissolved peptide sample from the giant salamander meat beforehand. This process is typically done manually, is cumbersome, and prone to inaccurate results due to operational errors. A new device is needed to solve these problems. Utility Model Content

[0003] To address the aforementioned issues, this invention proposes a device for detecting the purity of salamander peptides, comprising a crushing cylinder, an injection mechanism connected to the upper side of the crushing cylinder, a threaded connection at the lower end of the crushing cylinder to the top interface of a centrifuge cylinder, a discharge pipe with a control valve at the bottom of the crushing cylinder extending into a filter cartridge within the centrifuge cylinder, an output end of a centrifugal motor connected to the center of the bottom surface of the filter cartridge, a weighing ring mechanism provided on the bottom surface of the crushing cylinder, a boss at the center of the bottom surface of the crushing cylinder rotatably connected to the shaft of a crushing blade, a drive mechanism connected to the top surface of the crushing blade shaft, and all mechanisms and control valves electrically connected to a control panel.

[0004] Furthermore, the ring mechanism includes a grid ring, the inner ring of which is vertically slidably connected to the outer arc surface of the boss, the outer arc surface of the boss is provided with several branch pipes, the inlet of the branch pipes is located below the grid ring, the outlet of the branch pipes is connected to the discharge pipe, the bottom surface of the grid ring is in contact with the pressure-sensitive washer, and the pressure-sensitive washer is electrically connected to the control panel.

[0005] Furthermore, the drive mechanism includes a drive shaft with helical blades on its shaft body. The external spline at the bottom of the drive shaft mates with the internal spline on the top surface of the crusher shaft. The top of the drive shaft is rotatably connected to the center of the bottom surface of the cylinder cover. The rolled edge of the cylinder cover is threadedly connected to the feed inlet at the top of the crushing cylinder. A drive motor is installed at the center of the top surface of the cylinder cover. The output end of the drive motor is vertically downward connected to the top surface of the drive shaft. The drive motor is electrically connected to the control panel.

[0006] Furthermore, the liquid injection mechanism includes a liquid storage tank, a liquid pump is installed on the top of the liquid storage tank, the liquid pump is electrically connected to the control panel, the liquid pump input pipe extends into the liquid storage tank, and the liquid pump output pipe with control valve is connected to the connection port on the upper side of the crushing cylinder.

[0007] Furthermore, the bottom of the centrifuge cylinder is supported by feet, the centrifuge motor is installed at the center of the bottom surface of the centrifuge cylinder, and several liquid outlet pipes with control valves are arranged around the bottom surface of the centrifuge cylinder, with a filter screen at the inlet of the liquid outlet pipe.

[0008] The beneficial effects of this utility model are as follows: This utility model measures the mass of fish meat fed through a weighing ring mechanism, and automatically adds an appropriate amount of reagent to dissolve the peptides in the fish meat according to the amount of feed. The dissolved peptide turbidity is injected into a filter cartridge and centrifuged to remove insoluble matter. The obtained peptide solution is filtered through a filter screen and then injected into a test tube through an outlet tube for high-performance liquid chromatography analysis of peptide purity. It has the characteristics of simple operation and high degree of automation, which improves the detection efficiency of salamander peptide purity and avoids measurement distortion caused by human error. Attached Figure Description

[0009] Figure 1 This is a front view structural diagram of the present invention.

[0010] The reference numerals in the attached drawings are explained as follows: 1. Crushing cylinder; 101. Discharge pipe; 102. Boss; 103. Branch pipe; 2. Centrifuge cylinder; 201. Support leg; 202. Liquid outlet pipe; 203. Filter screen; 3. Filter cartridge; 4. Centrifugal motor; 5. Crushing blade; 6. Control panel; 7. Grille ring; 8. Pressure-sensitive washer; 9. Drive shaft; 901. Spiral blade; 10. Cylinder cover; 11. Drive motor; 12. Liquid storage tank; 13. Liquid pump. Detailed Implementation

[0011] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0012] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0013] The present invention will be further described below with reference to the accompanying drawings:

[0014] like Figure 1 As shown, the salamander peptide purity testing device includes a crushing cylinder 1. The lower end of the crushing cylinder 1 is threadedly connected to the top interface of a centrifuge cylinder 2. The bottom of the centrifuge cylinder 2 is supported by a support leg 201. A centrifuge motor 4 is installed at the center of the bottom surface of the centrifuge cylinder 2. Several outlet pipes 202 with control valves are arranged around the bottom surface of the centrifuge cylinder 2. A filter screen 203 is installed at the inlet of the outlet pipe 202. The discharge pipe 101 with a control valve at the bottom of the crushing cylinder 1 extends into the filter cylinder 3 in the centrifuge cylinder 2. The output end of the centrifuge motor 4 is connected to the center of the bottom surface of the filter cylinder 3.

[0015] In this embodiment, a weighing ring mechanism is provided on the bottom surface of the crushing cylinder 1. The weighing ring mechanism includes a grid ring 7. The inner ring of the grid ring 7 is vertically slidably connected to the outer arc surface of the boss 102 at the center of the bottom surface of the crushing cylinder 1. The outer arc surface of the boss 102 is provided with several branch pipes 103. The inlet of the branch pipe 103 is located below the grid ring 7, and the outlet of the branch pipe 103 is connected to the discharge pipe 101. The bottom surface of the grid ring 7 is in contact with the pressure-sensitive washer 8. The boss 102 is rotatably connected to the rotating shaft of the crushing blade 5. The inner spline on the top surface of the rotating shaft of the crushing blade 5 is engaged with the outer spline at the bottom of the drive shaft 9 in the drive mechanism. The shaft body of the drive shaft 9 is provided with a spiral blade 901. The top of the drive shaft 9 is rotatably connected to the center of the bottom surface of the cylinder cover 10. The rolled edge of the cylinder cover 10 is threadedly connected to the top inlet of the crushing cylinder 1. The center of the top surface of the cylinder cover 10 is equipped with a drive motor 11. The output end of the drive motor 11 is vertically downward connected to the top surface of the drive shaft 9.

[0016] In this embodiment, a liquid injection mechanism is connected to the upper side of the crushing cylinder 1. The liquid injection mechanism includes a storage tank 12, and a liquid pump 13 is provided at the top of the storage tank 12. The liquid pump 13 is electrically connected to the control panel 6. The input pipe of the liquid pump 13 extends into the storage tank 12, and the output pipe of the liquid pump 13 with a control valve is connected to the connection port on the upper side of the crushing cylinder 1. The control panel 6 is provided at the top of the storage tank 12, and the control panel 6 is electrically connected to the centrifugal motor 4, the pressure-sensitive washer 8, the drive motor 11, and various control valves.

[0017] The working principle of this utility model is as follows:

[0018] Add the giant salamander meat chunks into the crushing cylinder 1, screw the cylinder cover 10 onto the top feed port of the crushing cylinder 1, and connect the drive shaft 9 to the crushing blade 5 via a spline to complete the device assembly. The pressure-sensitive washer 8 transmits the measured meat chunk mass information to the control panel 6. The control panel 6 turns on the liquid pump 13 and injects an appropriate amount of peptide solvent (0.1% trifluoroacetic acid aqueous solution, with the concentration controlled at 0.4-0.6 mg / ml) into the crushing cylinder 1. Start the drive motor 11 to crush the meat chunks, dissolving the peptides in the meat chunks into the peptide solvent. During this process, the spiral blade 901 stirs the water flow downwards, which, while acting as a stirrer, forces the fish meat to be crushed more completely. After crushing and stirring for a period of time, turn off the drive motor 11, open the control valve on the discharge pipe 101, inject the peptide turbidity into the filter cartridge 3, start the centrifuge motor 4 to centrifuge the peptide turbidity, the insoluble matter in the turbidity is isolated in the filter cartridge 3, and the separated peptide solution flows into the bottom of the centrifuge cylinder 2. After centrifugation, open the control valve on the outlet pipe 202 to inject the peptide solution into the test tube, waiting to be used for high performance liquid chromatography analysis of peptide concentration.

[0019] This invention features simple operation and high degree of automation, which improves the detection efficiency of salamander peptide purity and avoids measurement distortion caused by human error.

[0020] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model.

Claims

1. A device for detecting the purity of peptides in giant salamanders, comprising a crushing cylinder (1), characterized in that: The upper side of the crushing cylinder (1) is connected to the liquid injection mechanism. The lower end of the crushing cylinder (1) is threaded to the top interface of the centrifugal cylinder (2). The discharge pipe (101) with the control valve at the bottom of the crushing cylinder (1) extends into the filter cylinder (3) in the centrifugal cylinder (2). The bottom center of the filter cylinder (3) is connected to the output end of the centrifugal motor (4). The bottom surface of the crushing cylinder (1) is provided with a weighing ring mechanism. The boss (102) at the center of the bottom surface of the crushing cylinder (1) is rotatably connected to the shaft of the crushing blade (5). The top surface of the shaft of the crushing blade (5) is connected to the drive mechanism. All mechanisms and control valves are electrically connected to the control panel (6).

2. The salamander peptide purity detection device according to claim 1, characterized in that: The weighing ring mechanism includes a grid ring (7), the inner ring of the grid ring (7) is vertically slidably connected to the outer arc surface of the boss (102), the outer arc surface of the boss (102) is provided with a number of branch pipes (103), the inlet of the branch pipe (103) is located below the grid ring (7), the outlet of the branch pipe (103) is connected to the discharge pipe (101), the bottom surface of the grid ring (7) is in contact with the pressure-sensitive washer (8), and the pressure-sensitive washer (8) is electrically connected to the control panel (6).

3. The salamander peptide purity detection device according to claim 1, characterized in that: The drive mechanism includes a drive shaft (9), the shaft body of the drive shaft (9) is provided with a spiral blade (901), the outer spline at the bottom of the drive shaft (9) is engaged with the inner spline on the top surface of the shaft of the crusher (5), the top of the drive shaft (9) is rotatably connected to the center of the bottom surface of the cylinder cover (10), the rolled edge of the cylinder cover (10) is threadedly connected to the feed port at the top of the crushing cylinder (1), the center of the top surface of the cylinder cover (10) is equipped with a drive motor (11), the output end of the drive motor (11) is vertically downward connected to the top surface of the drive shaft (9), and the drive motor (11) is electrically connected to the control panel (6).

4. The salamander peptide purity detection device according to claim 1, characterized in that: The liquid injection mechanism includes a liquid storage tank (12), a liquid pump (13) is provided on the top of the liquid storage tank (12), the liquid pump (13) is electrically connected to the control panel (6), the input pipe of the liquid pump (13) extends into the liquid storage tank (12), and the output pipe of the liquid pump (13) with a control valve is connected to the connection port on the upper side of the crushing cylinder (1).

5. The salamander peptide purity detection device according to claim 1, characterized in that: The bottom of the centrifuge cylinder (2) is supported by a support leg (201). The centrifuge motor (4) is installed at the center of the bottom surface of the centrifuge cylinder (2). The bottom surface of the centrifuge cylinder (2) is provided with several outlet pipes (202) with control valves. A filter screen (203) is provided at the inlet of the outlet pipe (202).

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