Perfusion module for multi-channel perfusion

Abstract

The utility model provides a multi-channel perfusion module, which is provided with at least two groups of sample carrying spaces for carrying biological tissue slice samples, and each group of sample carrying spaces are arranged separately; each group of sample loading space is respectively communicated with a liquid outlet; a liquid inlet flow channel is arranged to communicate the outside with each sample loading space, and a plurality of control valves which are independently controlled are arranged in the liquid inlet flow channel corresponding to each sample loading space. The perfusion module is provided with a plurality of groups of separated sample carrying spaces, and is matched with an independent control valve in a liquid inlet flow channel, so that batch one-time infiltration during perfusion infiltration of a plurality of biological tissue slice samples can be met, and meanwhile, a small number of biological tissue slice samples can be controlled in a single sample carrying space to be independently infiltrated; the perfusion module can meet the control requirements of perfusion infiltration treatment of different numbers of biological tissue slice samples, and reasonable distribution control of a perfusion solution is achieved.

Description

Technical Field

[0001] This utility model relates to the field of biological tissue section sample perfusion technology, specifically, to a multi-channel perfusion module. Background Technology

[0002] The perfusion of biological tissue sections under in vitro conditions directly affects the cell viability of the sections during subsequent cryopreservation, depending on factors such as appropriate temperature control, sufficient tissue section infiltration, and stable perfusion solution flow. Existing perfusion devices suffer from challenges such as difficulty in standardizing perfusion conditions, large fluctuations in experimental results, and difficulty in controlling experimental reproducibility and consistency. These factors significantly impact the cell viability of cryopreserved biological tissue sections. Therefore, improving the stability and reproducibility of perfusion has been a pressing issue in this field. Utility Model Content

[0003] This invention provides a multi-channel irrigation module to overcome the shortcomings of the prior art.

[0004] The multi-channel perfusion module is provided with at least two sets of sample-carrying spaces for carrying biological tissue slices, and each set of sample-carrying spaces is separated; each set of sample-carrying spaces is connected to a drain port; an inlet channel is provided to connect to the outside to each of the sample-carrying spaces, and multiple independently controlled control valves are provided in the inlet channel corresponding to each of the sample-carrying spaces.

[0005] Furthermore, the perfusion module includes: a sample carrier plate, with each sample carrier space disposed on the sample carrier plate; a liquid inlet plate, pressed against the sample carrier plate to form a sealed space for each sample carrier space; a liquid inlet channel disposed on the liquid inlet plate; and each control valve disposed on the liquid inlet plate.

[0006] Furthermore, each of the sample-carrying spaces is connected to a flow divider; the liquid inlet channel is connected to the outside to each of the flow dividers, and the output end of each of the control valves is provided corresponding to each flow divider.

[0007] Furthermore, the liquid inlet channel includes a liquid inlet, a liquid outlet, and a liquid inlet passage connecting the liquid inlet and the liquid outlet on the liquid inlet plate; multiple liquid outlets are provided and located on the lower side of the liquid inlet plate corresponding to each of the flow distribution chambers, and each of the control valves is connected to the liquid inlet passage and the output end of each control valve is provided corresponding to each liquid inlet outlet.

[0008] Furthermore, the liquid inlet is located on the outer periphery of the liquid inlet plate, the liquid inlet channel includes a horizontally extending cross channel on the rear side of the liquid inlet plate, the cross channel is provided with a flow channel outlet, and multiple liquid inlet branch channels are evenly distributed and diverted between the flow channel outlet and each liquid inlet outlet, and each control valve is provided corresponding to each liquid inlet branch channel.

[0009] Furthermore, the sample carrying space includes a sample carrying cavity, the cross-section of which is elliptical.

[0010] Furthermore, in the same sample carrying space, multiple sample carrying cavities are provided, and each sample carrying cavity is arranged in a straight line along its length direction, with adjacent sample carrying cavities connected by an immersion channel; the immersion channel includes a connecting straight channel that is arranged at the length end between two adjacent sample carrying cavities.

[0011] Furthermore, the sample-carrying spaces in each group are arranged horizontally along the front-to-back direction, and the sample-carrying spaces in each group are parallel to each other.

[0012] Furthermore, each of the sample-carrying spaces is surrounded by a positioning wall; it also includes a positioning sealing plate, which is adapted to be inserted into the positioning wall to form an upper enclosure of the sample-carrying space; the positioning sealing plate is set one-to-one with each sample-carrying space; the enclosing positioning wall is rectangular in shape.

[0013] Furthermore, it also includes a temperature control plate, which is disposed on the lower side of the liquid inlet plate and forms a heat exchange relationship with each of the sample carrying spaces; the temperature control plate is provided with a downwardly inclined drainage slope corresponding to the position of the liquid outlet.

[0014] The beneficial effects of this utility model are as follows:

[0015] The perfusion module, with its multiple separate sample-carrying spaces and independent control valves in the inlet channel, can simultaneously perform batch perfusion of multiple biological tissue slices and control a small number of biological tissue slices for independent perfusion in a single sample-carrying space. This allows the perfusion module to meet the control requirements for perfusion and perfusion of different numbers of biological tissue slices and achieve reasonable distribution and control of the perfusion solution.

[0016] The perfusion module can be adapted to combine different sample carrier plates and infusion plates according to the biological tissue section samples and tissue types, meeting the perfusion and wetting requirements of external perfusion solutions for biological tissue section samples. It utilizes a temperature control plate for heat transfer and an infusion plate for liquid injection and diversion, ensuring consistency and repeatability of the perfusion operation. Attached Figure Description

[0017] Figure 1 This is an exploded structural diagram of the irrigation module of this utility model;

[0018] Figure 2 This is a cross-sectional structural diagram of the irrigation module of this utility model.

[0019] Explanation of reference numerals in the attached figures:

[0020] Sample carrier 1; Sample carrier space 11, Sample carrier cavity 111, Immersion channel 12, Drain outlet 13, Diversion cavity 14, Positioning wall 15, Positioning sealing plate 16, Diversion port 161

[0021] Liquid inlet plate 2; liquid inlet channel 21, liquid inlet 22, liquid inlet outlet 23, liquid inlet passage 24, crossflow channel 241, channel outlet 242, liquid inlet branch channel 243.

[0022] Control valve 3; slotted valve 31

[0023] Temperature control plate 4; drainage ramp 41. Detailed Implementation

[0024] To make the technical solution, purpose and advantages of this utility model clearer, the following explanation is given in conjunction with the accompanying drawings and embodiments.

[0025] like Figures 1 to 2 As shown, for the perfusion application of biological tissue slice samples, this utility model provides a perfusion module, which is provided with at least two sets of sample carrying spaces 11 for carrying biological tissue slice samples, and each set of sample carrying spaces 11 is separated; each set of sample carrying spaces 11 is connected to a drain port 13; a liquid inlet channel 21 is provided to connect to the outside to each of the sample carrying spaces 11, and multiple independently controlled control valves 3 are provided in the liquid inlet channel 21 corresponding to each of the sample carrying spaces 11.

[0026] In application, biological tissue slices are placed in the sample-carrying space 11 of the perfusion module. An external infusion device connects to the inlet channel 21, allowing the perfusion solution to be input into the biological tissue slices in the sample-carrying space 11. The perfused solution is discharged from the outlet 13, thus allowing the solution to be used for perfusion to be input into the sample-carrying space 11 for continuous perfusion. Multiple sample-carrying spaces 11 are each equipped with a corresponding control valve 3. The opening and closing of the control valve 3 controls the start and stop of the perfusion solution flow into the corresponding sample-carrying space 11, satisfying the need for on-demand control of the input perfusion solution to specific locations within the sample-carrying space 11.

[0027] Example:

[0028] In this embodiment, a preferred structural configuration of the irrigation module is described.

[0029] The perfusion module includes a rectangular plate-shaped sample carrier plate 1 and a liquid inlet plate 2. Each sample carrier space 11 is disposed on the sample carrier plate 1, and each sample carrier space 11 includes multiple sample carrier cavities 111 formed by the upper and lower recesses of the sample carrier plate 1. The cross-section of each sample carrier cavity 111 is elliptical and has an upper opening. Each group of sample carrier spaces 11 is arranged horizontally along the front-to-back direction and is parallel to each other. In the same group of sample carrier spaces 11, multiple sample carrier cavities 111 are arranged in a straight line along their length direction, and adjacent sample carrier cavities 111 are connected by an immersion channel 12. The immersion channel 12 includes a connecting straight channel that connects two adjacent sample carrier cavities 111 at their length ends. The arrangement of the sample-carrying spaces 11 ensures that the irrigation solution can flow from back to front through the sample-carrying spaces 11 during the irrigation process, thus guaranteeing the fluidity and efficiency of the irrigation. At the same time, it ensures that the irrigation solution fills each sample-carrying space 11, thereby guaranteeing the irrigation effect.

[0030] The drain port 13 is connected to the foremost sample chamber 111 in each sample space 11, located in front of the sample plate 1.

[0031] Each sample-carrying space 11 has a flow divider 14 connected to the rear side of the last sample-carrying chamber 111. The inlet channel 21 connects to the outside to each flow divider 14, and the output end of each control valve 3 is set corresponding to each flow divider 14. The application of the flow divider 14 ensures the stability of the perfusion solution input into each sample-carrying space 11.

[0032] Each sample-carrying space 11 is surrounded by a positioning wall 15; it also includes a positioning sealing plate 16, which is adapted to be inserted into the positioning wall 15 to form an upper enclosure of the sample-carrying space 11; the positioning sealing plate 16 is set one-to-one with each sample-carrying space 11; the enclosing positioning wall 15 is rectangular in shape, each positioning sealing plate 16 is rectangular in shape, and each positioning sealing plate 16 is provided with a diversion port 161 corresponding to each diversion cavity 14.

[0033] It also includes an inlet plate 2 for pressing against the sample carrier plate 1, so that each sample carrier space 11 forms a sealed space; with the positioning sealing plate 16 provided, the positioning sealing plate 16 is first used to seal each sample carrier space 11, and then the inlet plate 2 is pressed down to complete the pressing and sealing of the sample carrier space 11. The inlet flow channel 21 is provided on the inlet plate 2 for organization, and each of the control valves 3 is provided on the inlet plate 2.

[0034] Specifically, the liquid inlet channel 21 includes a liquid inlet 22, a liquid inlet 23, and a liquid inlet passage 24 connecting the liquid inlet 22 and the liquid inlet 23, all disposed on the liquid inlet plate 2. The liquid inlet 22 is disposed on the outer periphery of the liquid inlet plate 2. The liquid inlet passage 24 includes a horizontally extending crossflow channel 241 on the rear side of the liquid inlet plate 2. A flow channel outlet 242 is disposed on the crossflow channel 241. Multiple liquid inlet branch channels 243 are evenly distributed and diverted between the flow channel outlet 242 and each liquid inlet outlet 23. Each control valve 3 is disposed corresponding to each liquid inlet branch channel 243. The multiple liquid inlet outlets 23 are disposed on the lower side of the liquid inlet plate 2, corresponding to each of the diversion chambers 14, so that the output end of each control valve 3 is disposed corresponding to the position of each diversion chamber 14.

[0035] In this embodiment, the control valve 3 is positioned on the upper side of the inlet plate 2 with a control end for controlling its opening and closing. Specifically, the control end is configured to be controlled by a rotary knob 31 for raising and lowering the valve structure using a screwdriver, thereby achieving the opening and closing of the control valve 3.

[0036] To meet the temperature control requirements during the perfusion process, the perfusion module also includes a temperature control plate 4. The temperature control plate 4 is located on the lower side of the sample carrier plate 1 and forms a heat exchange relationship with each of the sample carrier spaces 11. A drainage ramp 41, sloping downwards towards the front, is provided on the front side of the temperature control plate 4 corresponding to the drain port 13. The drainage ramp 41 facilitates the drainage of the tissue perfusion solution.

[0037] The above description is only a preferred embodiment of the present utility model. For those skilled in the art, modifications can still be made to the embodiments without departing from the implementation principle of the present utility model, and the corresponding modifications should also be considered within the protection scope of the present utility model.

Claims

1. A multi-channel irrigation module, characterized in that, At least two sets of sample-carrying spaces are provided for carrying biological tissue slices, and each set of sample-carrying spaces is separated; each set of sample-carrying spaces is connected to a drain port; a liquid inlet channel is provided to connect the outside to each of the sample-carrying spaces, and multiple independently controlled control valves are provided in the liquid inlet channel corresponding to each of the sample-carrying spaces.

2. The irrigation module as described in claim 1, characterized in that, include: A sample carrier plate, wherein each of the sample carrier spaces is disposed on the sample carrier plate; The liquid inlet plate is pressed against the sample carrier plate to form a sealed space for each sample carrier space; the liquid inlet channel is disposed on the liquid inlet plate, and each of the control valves is disposed on the liquid inlet plate.

3. The irrigation module as described in claim 2, characterized in that, Each of the sample-carrying spaces is connected to a flow divider; the liquid inlet channel is connected to the outside to each of the flow dividers, and the output end of each of the control valves is set corresponding to each flow divider.

4. The irrigation module as described in claim 3, characterized in that, The liquid inlet channel includes a liquid inlet, a liquid outlet, and a liquid inlet channel connecting the liquid inlet and the liquid outlet on the liquid inlet plate; multiple liquid outlets are provided and are located on the lower side of the liquid inlet plate corresponding to each of the flow distribution chambers; each of the control valves is connected to the liquid inlet channel and the output end of each of the control valves is provided corresponding to each liquid inlet outlet.

5. The irrigation module as described in claim 4, characterized in that, The liquid inlet is located on the outer periphery of the liquid inlet plate. The liquid inlet channel includes a horizontally extending cross channel on the rear side of the liquid inlet plate. A flow channel outlet is provided on the cross channel. Multiple liquid inlet branch channels are evenly distributed and diverted between the flow channel outlet and each liquid inlet outlet. Each control valve is provided corresponding to each liquid inlet branch channel.

6. The irrigation module as described in claim 1, characterized in that, The sample carrying space includes a sample carrying cavity, the cross-section of which is elliptical.

7. The irrigation module as described in claim 6, characterized in that, In the same sample carrying space, multiple sample carrying cavities are provided, and each sample carrying cavity is arranged in a straight line along its length direction. Adjacent sample carrying cavities are connected by an immersion channel; the immersion channel includes a connecting straight channel that is arranged at the length end between two adjacent sample carrying cavities.

8. The irrigation module as described in any one of claims 1 to 7, characterized in that, The sample-carrying spaces in each group are arranged horizontally along the front-to-back direction, and the sample-carrying spaces in each group are parallel to each other.

9. The irrigation module as described in claim 8, characterized in that, Each sample-carrying space is surrounded by a positioning wall; it also includes a positioning sealing plate, which is adapted to be inserted into the positioning wall to enclose the upper side of the sample-carrying space; the positioning sealing plate is set one-to-one with each sample-carrying space; the enclosing positioning wall is rectangular in shape.

10. The irrigation module as described in claim 2, characterized in that, It also includes a temperature control plate, which is disposed on the lower side of the liquid inlet plate and forms a heat exchange relationship with each of the sample carrying spaces; the temperature control plate is provided with a downwardly inclined drainage slope corresponding to the position of the liquid outlet.

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