Processing device for tissue samples
By designing a processing device comprising a first container and a second container, and utilizing the synergistic effect of through holes and hollowed-out areas, combined with a flexible friction layer, the problems of low tissue sample separation efficiency and cross-contamination are solved, achieving efficient separation and saving consumables.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- SUZHOU YUANYI STEM CELL TECHNOLOGY CO LTD
- Filing Date
- 2025-05-23
- Publication Date
- 2026-05-15
AI Technical Summary
Existing technologies have low separation efficiency for tissue samples, easily form gel-like mixtures, pose a high risk of cross-contamination, and result in increased consumable costs and high sample loss rates due to redundant operations.
Design a processing device comprising a first container and a second container, wherein the second container is inverted inside the first container, has a through hole at the top and a hollowed-out area on the side wall, and is combined with a flexible friction layer, utilizing the synergistic effect of gravity and liquid flow to achieve separation and cleaning of tissue samples, avoiding secondary contamination.
It achieves efficient separation of tissue samples, reduces the risk of cross-contamination, reduces the use of consumables, simplifies the operation process, and reduces costs.
Smart Images
Figure CN224247425U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of experimental equipment technology, and in particular to a processing device for tissue samples. Background Technology
[0002] Currently, the demand for tissue sample pretreatment is becoming increasingly sophisticated, and a routine technical mode of separation using a glassware-assisted cell scraper has gradually been formed. This technique achieves tissue disruption through physical scraping and completes sample purification in conjunction with rinsing operations. It has the technical characteristics of being intuitive to operate and having low equipment requirements, and has become a basic laboratory processing method.
[0003] In related technologies, a typical processing procedure is as follows: tissue samples are repeatedly scraped with a scraper in an open dish, and the sloughed material is removed by liquid rinsing, achieving coarse solid-liquid separation through gravity settling. When processing blood-stained or highly viscous tissues, operators need to transfer the sample to new dishes multiple times for gradient cleaning.
[0004] However, the specific problems with the above processing method are as follows: First, the separation efficiency is defective, as blood cell fragments and viscous matrix easily form a gel-like mixture, making it difficult to achieve effective stratification by gravity alone; second, there is a risk of cross-contamination, as the backflow of the rinsing solution can carry away the detached viscous substances and re-adhere to the sample surface, causing secondary contamination; third, there is operational redundancy, as processing a single sample requires multiple vessels for gradient cleaning, which greatly increases the cost of experimental consumables, and frequent transfer operations also increase the sample loss rate. Utility Model Content
[0005] In view of the shortcomings of the existing production technology, the applicant provides a processing device for tissue samples, which facilitates the separation of blood cell fragments from the viscous matrix during the processing, and the rinsing process does not contaminate the tissue samples, while also saving consumables.
[0006] The technical solution adopted in this utility model is as follows:
[0007] A processing apparatus for tissue samples, comprising:
[0008] The first container is hollow inside and has its opening facing upwards;
[0009] The second container is hollow inside and has its opening facing downwards. The opening end of the second container is fixed to the bottom surface of the first container, forming an integrated processing structure.
[0010] The top of the second container is provided with multiple through holes for the passage of tissue sample removal material;
[0011] The second container has at least one perforated area on its side wall to allow the cleaning material entering the second container to be discharged with the rinsing liquid;
[0012] The bottom outer surface of the first container is provided with a friction layer to increase the friction with the operating table;
[0013] The height of the second container is greater than the height of the first container.
[0014] As a further improvement to the above technical solution:
[0015] In one embodiment, the first container includes a first container body and a first assembly area disposed on the bottom surface of the first container body; the friction layer includes a flexible friction body and a second assembly area disposed on its top surface; the first assembly area and the second assembly area are detachably connected, such that the flexible friction body is disposed on the bottom surface of the first container body.
[0016] In one embodiment, the first assembly area and the second assembly area are connected by an adhesive bonding method.
[0017] In one embodiment, the first assembly area and the second assembly area are connected by magnetic attraction.
[0018] In one embodiment, the second container includes a second container body, the opening of which faces and is inverted inside the first container body, and the diameter of the through hole at the top of the second container body is 3-5 mm.
[0019] In one embodiment, the multiple through holes on the top of the second container are evenly distributed, and the spacing between two adjacent through holes is 5-10 mm.
[0020] In one embodiment, the hollowed-out area is distributed circumferentially along the side wall of the second container, and the hollowed-out area has a strip-shaped opening.
[0021] In one embodiment, the first container and the second container are shaped as a circle, an ellipse, or a polygon.
[0022] In one embodiment, the first and second containers are transparent structures made of transparent medical plastic to facilitate observation during processing.
[0023] In one embodiment, the flexible friction body of the friction layer is made of silicone or rubber to ensure the stability of the entire processing device during the process.
[0024] The beneficial effects of this utility model are as follows:
[0025] This invention features a compact structure and convenient operation. By opening a perforation at the top of the second container, tissue fragments and blood cells can pass through, while the hollowed-out area on the side wall of the second container facilitates the discharge of the cleaning fluid carrying away the removed material. Utilizing the synergistic effect of gravity and liquid flow, it effectively separates the viscous matrix from the target tissue sample. Specifically, the combination of the perforation and the hollowed-out area forms a "top-down" liquid flow path, allowing the cleaning fluid to efficiently discharge the cleaning material, ensuring that the tissue sample remains at the top of the second container while the cleaning material remains inside the first container, effectively separating them and eliminating the possibility of secondary adhesion and contamination.
[0026] This utility model also has the following advantages:
[0027] (1) This utility model inverts the second container into the first container to form an integrated processing space. During the operation, there is no need to frequently transfer the sample to different containers, which reduces the risk of contamination and the amount of consumables used, meeting the economic needs of the laboratory.
[0028] (2) The height of the second container of this utility model is greater than that of the first container. The height difference forms a protruding operation and processing area, which can also promote a more thorough stratification and rinsing effect.
[0029] (3) A flexible friction layer is added to the outer side of the bottom surface of the first container of this utility model. It is made of silicone or rubber, which significantly increases the friction with the operating table and prevents the sample from splashing out or the operation from being interrupted due to the sliding of the container during the processing. At the same time, the friction layer can be easily replaced or cleaned by bonding or magnetic connection between the first assembly area and the second assembly area, which extends the service life of the device and can also adapt to the surface characteristics of different operating tables. Attached Figure Description
[0030] Figure 1 This is a schematic diagram of the overall structure of this utility model.
[0031] Figure 2 for Figure 1 A schematic diagram of the explosion state.
[0032] Figure 3 This is a schematic diagram of the specific structure of the second container of this utility model.
[0033] Figure 4 This is a schematic diagram of the fit between the first container and the friction layer of this utility model.
[0034] Wherein: 100, first container; 200, second container; 300, friction layer;
[0035] 110. First container body; 120. First assembly area;
[0036] 210. Second container body; 220. Through hole; 230. Hollowed-out area;
[0037] 310. Flexible friction body; 320. Second assembly area. Detailed Implementation
[0038] The specific embodiments of this utility model are described below with reference to the accompanying drawings.
[0039] To facilitate understanding of this utility model, a more complete description will be given below with reference to the accompanying drawings. Preferred embodiments of this utility model are shown in the drawings. However, this utility model can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a more thorough and complete understanding of the disclosure of this utility model.
[0040] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.
[0041] When using the terms “including,” “having,” and “comprising” as described herein, another component may be added unless explicitly qualifying terms such as “only,” “consisting of,” etc. are used. Unless otherwise stated, singular terms may include plural forms and should not be construed as having a quantity of one.
[0042] It should be understood that although the terms "first," "second," etc., may be used herein to describe various elements, these elements should not be limited by these terms. These terms are used only to distinguish one element from another. For example, without departing from the scope of this invention, a first element may be referred to as a second element, and similarly, a second element may be referred to as a first element.
[0043] Furthermore, the accompanying drawings are not drawn to a 1:1 scale, and the relative dimensions of the components are shown in the drawings only as examples and not necessarily to actual scale.
[0044] like Figures 1-4 The accompanying drawing shows a schematic diagram of the structural state of a tissue sample processing device according to an embodiment of the present invention; for ease of description, the drawing only shows the structure related to the embodiment of the present invention.
[0045] This application provides a processing device for tissue samples, including a first container 100, a second container 200, and a friction layer 300.
[0046] In this embodiment, the first container 100 includes a first container body 110 and a first assembly area 120, and has a hollow structure with the opening facing upward; the first assembly area 120 is provided on the bottom surface of the first container body 110 for connection with the friction layer 300.
[0047] Furthermore, the shape of the first container 100 can be circular, elliptical, or polygonal, preferably circular to suit routine laboratory operating habits; even further, the first container 100 is made of transparent medical plastic to facilitate real-time observation of the internal processing.
[0048] In this embodiment, the second container 200 includes a second container body 210, which is inverted inside the first container body 110 with its opening facing downwards and is fixed to form an integrated processing structure. The height of the second container 200 is greater than the height of the first container 100, forming a height difference that facilitates the formation of an operating area on the top surface of the second container 200. Multiple through holes 220 are evenly opened on the top of the second container body 210, with a hole diameter of 3-5 mm and a spacing of 5-10 mm between adjacent through holes, allowing tissue debris and removal materials (such as blood cells and viscous matrix) to pass through.
[0049] Furthermore, the second container body 210 has strip-shaped open perforated areas 230 distributed circumferentially on the side wall to guide the flushing fluid carrying the cleaned material out.
[0050] Furthermore, the width of the hollowed-out area 230 is set to 5mm, and its length covers 2 / 3 of the side wall height to ensure efficient drainage.
[0051] In this embodiment, the friction layer 300 includes a flexible friction body 310 and a second assembly area 320; wherein, the flexible friction body 310 is made of silicone or rubber and has anti-slip texture on its surface, which significantly increases the friction with the operating table; the second assembly area 320 is located on the top surface of the flexible friction body 310 and is detachably connected to the first assembly area 120 by an adhesive or magnetic element.
[0052] In practical application, the working method of this utility model is as follows:
[0053] Step 1: Place the tissue sample to be processed in the top area of the second container 200;
[0054] Step 2: Use a cell scraper to scrape the sample, and the resulting fragments fall into the second container 200 through the through-hole 220;
[0055] Step 3: Inject the rinsing solution. The liquid carries the removed material through the perforated area 230 into the first container 100. The target tissue sample, due to its large size, cannot pass through the perforation and remains at the top of the second container 200.
[0056] Step 4: After the stratification is completed, directly pour out the waste liquid in the first container 100. There is no need to transfer the tissue sample to avoid contamination.
[0057] In some embodiments, the friction layer 300 can be disassembled for cleaning or replacement according to the different surface characteristics of the worktable to adapt to different surfaces.
[0058] This invention has a reasonable structure and is easy to operate. Through the synergistic effect of the through hole 220 and the hollow area 230, a liquid flow path is formed from top to bottom. The combination of gravity and fluid dynamics enables rapid stratification of the viscous matrix and the target tissue. The friction layer 300 is also used to provide high friction to prevent the device from sliding during operation.
[0059] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.
[0060] The embodiments described above merely illustrate the implementation of this utility model, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the utility model patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these all fall within the protection scope of this utility model. Therefore, the protection scope of this utility model patent should be determined by the appended claims.
Claims
1. A processing apparatus for tissue samples, characterized in that, include: The first container (100) is hollow inside and has its opening facing upward; The second container (200) is hollow inside and has its opening facing downwards. The opening end of the second container (200) is fixed to the bottom surface of the first container (100) to form an integrated processing structure. The top of the second container (200) is provided with a plurality of through holes (220) for the passage of tissue sample removal material; The second container (200) has at least one perforated area (230) on its side wall for allowing the cleaning material entering the second container (200) to be discharged with the rinsing liquid; The bottom outer surface of the first container (100) is provided with a friction layer (300) to increase the friction with the operating table; The height of the second container (200) is greater than the height of the first container (100).
2. The processing apparatus for tissue samples according to claim 1, characterized in that, The first container (100) includes a first container body (110) and a first assembly area (120) disposed on the bottom surface of the first container body (110); The friction layer (300) includes a flexible friction body (310) and a second assembly area (320) disposed on its top surface; The first assembly area (120) and the second assembly area (320) are detachably connected, so that the flexible friction body (310) is disposed on the bottom surface of the first container body (110).
3. The apparatus for processing tissue samples according to claim 2, characterized in that, The first assembly area (120) and the second assembly area (320) are connected by adhesive bonding.
4. The processing apparatus for tissue samples according to claim 2, characterized in that, The first assembly area (120) and the second assembly area (320) are connected by magnetic attraction.
5. The apparatus for processing tissue samples according to claim 1, characterized in that, The second container (200) includes a second container body (210), the opening of the second container body (210) facing and inverted inside the first container body (110), and the diameter of the through hole (220) at the top of the second container body (210) is 3-5mm.
6. The processing apparatus for tissue samples according to claim 5, characterized in that, The second container (200) has multiple through holes (220) evenly distributed on the top, and the distance between two adjacent through holes (220) is 5-10 mm.
7. The processing apparatus for tissue samples according to claim 1, characterized in that, The hollow area (230) is distributed circumferentially along the side wall of the second container (200), and the structure of the hollow area (230) is a strip-shaped opening.
8. The apparatus for processing tissue samples according to claim 1, characterized in that, The first container (100) and the second container (200) are either circular, elliptical or polygonal in shape.
9. The apparatus for processing tissue samples according to claim 8, characterized in that, The first container (100) and the second container (200) are transparent structures made of transparent medical plastic, which facilitates observation during the processing.
10. The processing apparatus for tissue samples according to claim 1, characterized in that, The flexible friction body (310) of the friction layer (300) is made of silicone or rubber to ensure the stability of the entire processing device during the process.