Microorganism culture device
By employing a stirring shaft and hollow stirring rod design in the microbial culture device, combined with an aeration disc and a rotating air supply assembly, independent gas-liquid dual-path delivery is achieved, solving the problems of low nutrient solution mixing efficiency and unstable dissolved oxygen, and providing an efficient and stable microbial culture environment.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- HUIMIN COUNTY JURUN AGRI TECH CO LTD
- Filing Date
- 2025-06-06
- Publication Date
- 2026-05-19
AI Technical Summary
Existing microbial culture devices have low mixing efficiency during nutrient solution addition, making it difficult to provide stable dissolved oxygen. This results in an uneven microbial growth environment, increasing cleaning difficulty and the risk of contamination.
The design incorporates a stirring shaft and hollow stirring rod, combined with an aeration disc and a rotating air supply assembly, to achieve independent gas-liquid dual-path delivery, ensuring that stirring and aeration occur simultaneously, forming a synergistic gas-liquid mixing effect, simplifying the structure and reducing cleaning difficulty.
It significantly improves the mixing efficiency of nutrient solution and water, provides a continuous and stable supply of dissolved oxygen, reduces the risk of nutrient solution residue and microbial contamination, and meets the needs of industrial continuous culture and precise control in the laboratory.
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Figure CN224258614U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of microbial culture technology, and in particular to a microbial culture device. Background Technology
[0002] In the field of microbial culture technology, the performance of the culture device is crucial to the growth, reproduction, and metabolic product generation of microorganisms. Existing microbial culture devices often struggle to simultaneously meet the multiple requirements of microorganisms for nutrient supply and growth environment. On the one hand, traditional culture devices often use a one-time, centralized feeding method during nutrient solution addition, resulting in the inability to quickly and uniformly mix the nutrient solution with the culture water. This makes it difficult for microorganisms to obtain a balanced nutritional environment in the early stages of cultivation, affecting their growth efficiency and activity. On the other hand, most devices lack the ability to regulate dissolved oxygen in the culture environment, failing to provide stable and suitable survival conditions for microorganisms and limiting their application in microbial culture scenarios with stringent requirements for culture conditions.
[0003] For example, patent CN215103287U discloses a microbial culture device, including an incubator. A motor is fixedly installed at the top center of the incubator, and a rotating rod is fixedly connected to the output end of the motor. A movable rod is fixedly connected to the surface of the rotating rod, and an air pump is fixedly installed at the bottom center of the incubator. In this invention, water and nutrient solution are poured into the incubator through the water inlet. The motor is started by a PLC controller, which drives the rotating rod to rotate the filter cloth, mixing the water and nutrient solution. The air pump is started by the PLC controller, transporting air to the inside of the air supply pipe, and then to the exhaust pipe.
[0004] In the above-mentioned scheme, the aeration device has a complex structure, which increases the difficulty of cleaning the incubator and easily leads to nutrient solution residue and microbial contamination. Furthermore, the stirring of the culture medium is carried out after the culture medium is directly poured into the water. Due to the initial aggregation of the culture medium, it takes a long time to stir it evenly, resulting in low mixing efficiency and making it difficult to meet the requirements of high-efficiency cultivation. Utility Model Content
[0005] To address the problems of complex structures in current aeration devices, which increase the difficulty of cleaning in the incubator and easily lead to nutrient solution residue and microbial contamination, this utility model provides a microbial culture device.
[0006] To solve the above problems, the technical solution adopted by this utility model is as follows:
[0007] A microbial culture device includes an incubator and a top cover. A stirring shaft is mounted on the top cover, with its lower end extending into the incubator and its upper end penetrating the top cover and connected to a drive mechanism. A stirring rod is radially arranged on the outer wall of the stirring shaft. The stirring rod is hollow and has an aeration disc mounted on it. A gas chamber is located inside the stirring shaft, communicating with the stirring rod. A rotary air supply assembly is mounted on the upper end of the stirring shaft in conjunction with the gas chamber. The design of the stirring shaft and hollow stirring rod, combined with the aeration disc mounted on the stirring rod, integrates aeration and stirring functions into one. Compared to traditional, complex aeration devices, this simplifies the internal structure of the incubator, reduces cleaning difficulty, and minimizes the risk of nutrient solution residue and microbial contamination. Simultaneously, the aeration disc releases gas evenly during stirring, accelerating the mixing of nutrient solution and water, preventing culture solution aggregation, and significantly improving mixing efficiency. The rotary air supply assembly ensures that gas is supplied to the gas chamber while the stirring shaft rotates.
[0008] Preferably, the rotary gas supply assembly includes a connecting hole 1 radially opened along the stirring shaft; the connecting hole 1 connects to the gas chamber; a sealing ring is rotatably mounted on the stirring shaft in conjunction with the connecting hole 1; a circular groove 1 is provided on the inner wall of the sealing ring; an air inlet pipe is radially arranged on the sealing ring; both the air inlet pipe and the connecting hole 1 connect to the circular groove 1. The connecting hole 1 radially arranged along the stirring shaft connects to the gas chamber, and in conjunction with the rotatably mounted sealing ring, the air inlet pipe and the connecting hole 1 form a dynamically sealed gas channel through the circular groove 1. This ensures continuous communication between the gas chamber and the external gas source when the stirring shaft rotates, and prevents gas leakage through the sealing ring structure. It ensures that the aeration process and the stirring action are synchronized, providing a continuous and stable supply of dissolved oxygen for microbial cultivation. At the same time, it simplifies the internal gas path layout of the device and improves the compactness and reliability of the overall structure.
[0009] Preferably, a second annular groove is provided on the outer wall of the stirring shaft to fit the sealing ring. This second annular groove, together with the first annular groove on the inner wall of the sealing ring, forms a double-sealing structure, effectively enhancing the sealing performance at the connection between the gas chamber and the air inlet pipe. This prevents gas leakage from gaps when the stirring shaft rotates, ensuring a stable gas supply through the air inlet pipe, the first annular groove, and the connecting hole, before being evenly released into the incubator via the hollow stirring rod and aeration disc. This design improves the reliability of the gas path system without significantly increasing structural complexity, ensuring that the aeration process is synchronized with and continuously stable with the stirring action. It guarantees the dissolved oxygen requirements of microorganisms and reduces redundant internal components through a compact sealing structure, further reducing cleaning and maintenance difficulties, and providing structural assurance for efficient and stable microbial culture.
[0010] Preferably, a liquid chamber is also provided inside the stirring shaft; both the gas chamber and the liquid chamber have annular cross-sections; the outer diameter of the liquid chamber is smaller than the inner diameter of the gas chamber; a rotary liquid supply assembly is provided at the upper end of the stirring shaft in conjunction with the liquid chamber; several liquid outlet pipes are provided on the outer wall of the stirring shaft; the liquid outlet pipes are connected to the liquid chamber. This achieves a compact structural design for independent gas-liquid dual-path delivery: the rotary liquid supply assembly and the rotary gas supply assembly are respectively coordinated with the liquid chamber and the gas chamber to ensure that nutrient solution and gas can be delivered synchronously and stably into the incubator when the stirring shaft rotates; the liquid chamber releases evenly through the liquid outlet pipes, forming a "gas-liquid synergistic mixing effect" with the gas released by the aeration plate during the stirring process. The gas stirs the water flow to generate turbulence, which drives the nutrient solution to diffuse. At the same time, the nutrient solution is more easily broken into tiny droplets under the impact of the airflow, significantly improving the mixing efficiency and uniformity. In addition, the annular cavity layout makes full use of the internal space of the stirring shaft, avoids the structural complexity caused by traditional external pipelines, reduces cleaning dead corners, reduces the risk of nutrient solution residue and contamination, and provides microorganisms with a dynamic culture environment with sufficient dissolved oxygen and balanced nutrition, meeting the dual needs of industrial continuous culture and precise control in the laboratory.
[0011] Preferably, the rotary liquid supply component is positioned above the rotary gas supply component; the drive mechanism includes a motor; the motor is connected to the stirring shaft via a transmission component; the transmission component is positioned between the rotary liquid supply component and the rotary gas supply component. This design facilitates external pipeline connections, reduces the number of openings at the top of the incubator, lowers the risk of contamination, and, through a drive, gas, and liquid separation mechanism, enables the stirring, aeration, and liquid supply functions to operate synchronously and controllably. It is suitable for microbial culture scenarios with high requirements for space utilization and ease of operation, and can significantly improve equipment integration and operational stability, especially in industrial continuous production.
[0012] Preferably, the rotary liquid supply assembly includes a threaded hole fixedly mounted on the top of the stirring shaft; a rotary joint is threaded into the threaded hole; the threaded hole and the liquid chamber are connected via a second connecting hole. The threaded hole fixed to the top of the stirring shaft and the liquid chamber are connected via the second connecting hole. The rotary joint, screwed into the threaded hole, forms a detachable sealed connection, facilitating quick connection to external liquid supply lines (such as storage tanks, peristaltic pumps, etc.) and preventing nutrient solution leakage through the tightness of the threaded fit. This ensures a continuous and stable inflow of nutrient solution into the liquid chamber via the second connecting hole, and then uniform release into the incubator through the outlet pipe.
[0013] Preferably, the outlet pipe and the stirring rod are staggered. The outlet pipe is distributed on the outer wall of the stirring shaft and is offset from the stirring rod, so that the nutrient solution is released from the middle or a specific area of the stirring shaft, while the aeration disc releases gas to the edge or different layers of the incubator through the stirring rod, forming a staggered flow field of "central nutrient supply + three-dimensional aeration". This layout allows the nutrient solution to diffuse in all directions under the centrifugal force of the rotating stirring shaft, and it crosses and impacts the lateral / longitudinal airflow generated by the aeration disc. This forces the nutrient solution droplets to break up more quickly under turbulence and disperse evenly in the culture water, avoiding the problems of excessively high local concentration or gas-liquid short-circuiting caused by traditional coaxial nutrient and gas supply.
[0014] Preferably, the transmission assembly includes a driven pulley keyed to the stirring shaft; the driven pulley is connected to a driving pulley via a synchronous belt; the driving pulley is keyed to the output shaft of the motor; and the motor is mounted on the upper cover.
[0015] As can be seen from the above technical solutions, the advantages of this utility model include:
[0016] 1. The design of the stirring shaft and hollow stirring rod, combined with the aeration disc mounted on the stirring rod, integrates aeration and stirring functions into one. Compared to traditional complex aeration devices, this simplifies the internal structure of the incubator, reduces cleaning difficulty, and minimizes the risk of nutrient solution residue and microbial contamination. Simultaneously, the aeration disc releases gas evenly during stirring, accelerating the mixing of nutrient solution and water, preventing culture solution aggregation, and significantly improving mixing efficiency. The rotating air supply component ensures that gas is supplied to the gas chamber while the stirring shaft rotates.
[0017] 2. The design of the liquid chamber and rotating liquid supply assembly enables a compact structure with independent gas-liquid dual-path delivery: the rotating liquid supply assembly and rotating gas supply assembly work in conjunction with the liquid chamber and gas chamber respectively, ensuring that nutrient solution and gas are delivered synchronously and stably into the incubator when the stirring shaft rotates; the liquid chamber releases liquid evenly through the outlet pipe, forming a "gas-liquid synergistic mixing effect" with the gas released from the aeration plate during the stirring process. The gas agitates the water flow, generating turbulence, which drives the diffusion of the nutrient solution. At the same time, the nutrient solution is more easily broken into tiny droplets under the impact of the airflow, significantly improving mixing efficiency and uniformity. In addition, the annular cavity layout makes full use of the internal space of the stirring shaft, avoiding the structural complexity problems caused by traditional external pipelines, reducing cleaning dead zones, and lowering the risk of nutrient solution residue and contamination. It provides microorganisms with a dynamic culture environment with sufficient dissolved oxygen and balanced nutrition, meeting the dual needs of industrial continuous culture and precise laboratory control. Attached Figure Description
[0018] To more clearly illustrate the technical solution of this utility model, the drawings used in the description will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0019] Figure 1 This is a schematic diagram of the structure of this utility model.
[0020] Figure 2 This is a partial cross-sectional structural diagram of the present invention.
[0021] Figure 3 This is a top view of the structure of the present invention after removing the incubator and the top cover.
[0022] Figure 4 for Figure 3 Schematic diagram of the sectional view along the AA direction.
[0023] Figure 5 for Figure 4 Enlarged diagram of point C in the middle.
[0024] Figure 6 for Figure 3 Schematic diagram of the BB-direction section.
[0025] Figure 7 This is a partial cross-sectional view of the stirring shaft of this utility model after the aeration disc is installed.
[0026] Figure 8 for Figure 7 Enlarged diagram of point D in the middle.
[0027] Figure 9 This is a partial cross-sectional view of the sealing ring of this utility model.
[0028] Explanation of reference numerals in the attached diagram: 1-Incubator, 2-Top cover, 3-Stirring shaft, 4-Aeration disc, 5-Sealing ring, 6-Motor, 7-Rotary joint, 8-Driven pulley, 9-Synchronous belt, 10-Driven pulley;
[0029] 301-Stirring rod, 302-Gas chamber, 303-Connecting hole one, 304-Circular groove two, 305-Liquid chamber, 306-Threaded hole, 307-Connecting hole two, 308-Liquid outlet pipe, 309-Connecting pipe; 501-Circular groove one, 502-Air inlet pipe. Detailed Implementation
[0030] To make the objectives, features, and advantages of this utility model more apparent and understandable, the technical solutions of this utility model will be clearly and completely described below with reference to the accompanying drawings of the specific embodiments. Obviously, the embodiments described below are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this patent, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this patent.
[0031] like Figure 1 , Figure 2 As shown, a microbial culture device includes an incubator 1 and a top cover 2. A stirring shaft 3 is provided on the top cover 2. The lower end of the stirring shaft 3 extends into the incubator 1, and the upper end of the stirring shaft 3 passes through the top cover 2 and is connected to a drive mechanism. The drive mechanism includes a motor 6. The motor 6 is connected to the stirring shaft 3 through a transmission assembly. The transmission assembly includes a driven pulley 8 that is keyed to the stirring shaft 3. The driven pulley 8 is connected to a driving pulley 10 through a synchronous belt 9. The driving pulley 10 is keyed to the output shaft of the motor 6. The motor 6 is mounted on the top cover 2.
[0032] like Figure 3 , Figure 4 and Figure 5 As shown, a stirring rod 301 is radially arranged on the outer wall of the stirring shaft 3; the stirring rod 301 is hollow; an aeration disc 4 is installed on the stirring rod 301; a gas chamber 302 is provided inside the stirring shaft 3; the gas chamber 302 is connected to the stirring rod 301; a rotating air supply component is provided at the upper end of the stirring shaft 3 in conjunction with the gas chamber 302.
[0033] The design of the stirring shaft 3 and the hollow stirring rod 301, combined with the aeration disc 4 mounted on the stirring rod 301, integrates aeration and stirring functions into one. Compared to traditional complex aeration devices, this simplifies the internal structure of the incubator 1, reduces cleaning difficulty, and minimizes the risk of nutrient solution residue and microbial contamination. Simultaneously, the aeration disc 4 releases gas evenly during stirring, accelerating the mixing of nutrient solution and water, preventing culture solution aggregation, and significantly improving mixing efficiency. The rotating air supply component ensures that gas is supplied to the gas chamber 302 while the stirring shaft 3 rotates.
[0034] Among them, such as Figure 7 , Figure 8 and Figure 9As shown, the rotary gas supply assembly includes a connecting hole 303 radially opened along the stirring shaft 3; the connecting hole 303 connects to the gas chamber 302; a sealing ring 5 is rotatably mounted on the stirring shaft 3 in conjunction with the connecting hole 303; a circular groove 501 is provided on the inner wall of the sealing ring 5; an air inlet pipe 502 is radially arranged on the sealing ring 5; both the air inlet pipe 502 and the connecting hole 303 connect to the circular groove 501. The connecting hole 303 radially arranged along the stirring shaft 3 connects to the gas chamber 302, and in conjunction with the rotatably mounted sealing ring 5, the air inlet pipe 502 and the connecting hole 303 form a dynamically sealed gas channel through the circular groove 501. This ensures continuous communication between the gas chamber 302 and the external gas source when the stirring shaft 3 rotates, and prevents gas leakage through the sealing ring 5 structure. This ensures that the aeration process and the stirring action are synchronized, providing a continuous and stable supply of dissolved oxygen for microbial cultivation. At the same time, it simplifies the internal gas path layout of the device and improves the compactness and reliability of the overall structure. A connecting pipe 309 is provided on the stirring rod 301, and an aeration disc 4 is installed on the connecting pipe 309. During use, an appropriate number of aeration discs 4 are installed as needed, and plugs are installed on the remaining unused connecting pipes 309. A second annular groove 304 is provided on the outer wall of the stirring shaft 3 in conjunction with the sealing ring 5. The second annular groove 304 and the first annular groove 501 on the inner wall of the sealing ring 5 form a double sealing structure, which effectively enhances the sealing performance at the connection between the gas chamber 302 and the air inlet pipe 502, prevents gas from leaking from the gap when the stirring shaft 3 rotates, and ensures that the gas source is stably input into the gas chamber 302 through the air inlet pipe 502, the first annular groove 501 and the connecting hole 303, and then evenly released into the incubator 1 through the hollow stirring rod 301 and the aeration disc 4. This design improves the reliability of the gas path system without significantly increasing structural complexity, making the aeration process synchronized and continuously stable with the stirring action. It not only ensures the dissolved oxygen requirements of microorganisms, but also reduces redundant components inside the device through a compact sealed structure, further reducing the difficulty of cleaning and maintenance, and providing structural protection for efficient and stable microbial culture.
[0035] like Figure 5-8As shown, a liquid chamber 305 is also provided inside the stirring shaft 3; both the gas chamber 302 and the liquid chamber 305 have annular cross-sections; the outer diameter of the liquid chamber 305 is smaller than the inner diameter of the gas chamber 302; a rotary liquid supply assembly is provided at the upper end of the stirring shaft 3 in conjunction with the liquid chamber 305; several liquid outlet pipes 308 are provided on the outer wall of the stirring shaft 3; the liquid outlet pipes 308 are connected to the liquid chamber 305. A compact structural design with independent gas and liquid dual-path delivery is realized: the rotary liquid supply assembly and the rotary gas supply assembly are respectively coordinated with the liquid chamber 305 and the gas chamber 302 to ensure that the nutrient solution and gas can be synchronously and stably delivered into the incubator 1 when the stirring shaft 3 rotates; the liquid chamber 305 releases evenly through the liquid outlet pipes 308, and forms a "gas-liquid synergistic mixing effect" with the gas released by the aeration plate 4 during the stirring process. The gas stirs the water flow to generate turbulence, which drives the nutrient solution to diffuse. At the same time, the nutrient solution is more easily broken into tiny droplets under the impact of the airflow, which significantly improves the mixing efficiency and uniformity. In addition, the annular cavity layout makes full use of the internal space of the stirring shaft 3, avoids the structural complexity caused by traditional external pipelines, reduces cleaning dead corners, reduces the risk of nutrient solution residue and contamination, and provides microorganisms with a dynamic culture environment with sufficient dissolved oxygen and balanced nutrition, meeting the dual needs of industrial continuous culture and precise control in the laboratory.
[0036] In the above configuration, the rotary liquid supply assembly is positioned above the rotary air supply assembly; the transmission assembly is positioned between the rotary liquid supply assembly and the rotary air supply assembly. This facilitates external pipeline connections, reduces the number of openings at the top of the incubator 1, lowers the risk of contamination, and, through a drive, gas, and liquid separation mechanism, enables the synchronous and controllable operation of stirring, aeration, and liquid supply functions. It is suitable for microbial culture scenarios with high requirements for space utilization and ease of operation, and can significantly improve equipment integration and operational stability, especially in industrial continuous production. The rotary liquid supply assembly includes a threaded hole 306 fixedly mounted at the top of the stirring shaft 3; a rotary joint 7 is threaded into the threaded hole 306; the threaded hole 306 and the liquid chamber 305 are connected through a second connecting hole 307. The threaded hole 306 fixed to the top of the stirring shaft 3 is connected to the liquid chamber 305 through the second connecting hole 307. After the rotary joint 7 is screwed into the threaded hole 306, a detachable sealed connection is formed, which facilitates quick connection to external liquid supply pipelines such as storage tanks and peristaltic pumps, and prevents nutrient solution leakage through the tightness of the threaded fit. Ensure that the nutrient solution is continuously and stably fed into the liquid chamber 305 through the connecting hole 2 307, and then evenly released into the incubator 1 through the outlet pipe 308.
[0037] The outlet pipe 308 and the stirring rod 301 are staggered. The outlet pipe 308 is distributed on the outer wall of the stirring shaft 3 and is staggered from the stirring rod 301, so that the nutrient solution is released from the middle or a specific area of the stirring shaft 3, while the aeration plate 4 releases gas to the edge or different layers of the incubator 1 through the stirring rod 301, forming a staggered flow field of "central liquid supply + three-dimensional aeration". This layout allows the nutrient solution to diffuse in all directions under the centrifugal force of the rotating stirring shaft 3, and then cross and impact the horizontal / vertical airflow generated by the aeration plate 4. This forces the nutrient solution droplets to break up more quickly under the action of turbulence and disperse evenly in the culture water, avoiding the problem of excessively high local concentration or gas-liquid short circuit caused by traditional coaxial liquid and gas supply. In this embodiment, the stirring rod 301 is arranged in five layers, with two stirring rods 301 in each layer, and the included angle between the two stirring rods 301 is 180°. There are four liquid outlet pipes 308, each of which is located between two layers of stirring rods 301, and the center line of the liquid outlet pipe 308 is at a 90° angle to the center line of the stirring rod 301.
[0038] The above description of the disclosed embodiments enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims
1. A microbial culture device comprising a culture box (1) and an upper cover (2), wherein a stirring shaft (3) is arranged on the upper cover (2), the lower end of the stirring shaft (3) extends into the culture box (1), and the upper end of the stirring shaft (3) is connected with a driving mechanism after penetrating through the upper cover (2), characterized in that, A stirring rod (301) is radially arranged on the outer wall of the stirring shaft (3); the stirring rod (301) is hollow; an aeration disc (4) is installed on the stirring rod (301); a gas chamber (302) is arranged inside the stirring shaft (3); the gas chamber (302) is connected to the stirring rod (301); a rotating gas supply component is arranged at the upper end of the stirring shaft (3) in conjunction with the gas chamber (302).
2. The microorganism culture device according to claim 1, characterized by The rotary gas supply assembly includes a connecting hole (303) radially opened along the stirring shaft (3); the connecting hole (303) connects to the gas chamber (302); a sealing ring (5) is rotatably arranged on the stirring shaft (3) in conjunction with the connecting hole (303); a circular groove (501) is provided on the inner wall of the sealing ring (5); an air inlet pipe (502) is provided radially along the sealing ring (5); the air inlet pipe (502) and the connecting hole (303) both connect to the circular groove (501).
3. The microbial culture apparatus according to claim 2, characterized by The outer wall of the stirring shaft (3) is fitted with a sealing ring (5) and has a circular groove (304).
4. The microorganism culture device according to claim 3, characterized by The stirring shaft (3) is also provided with a liquid chamber (305); the cross-sections of the gas chamber (302) and the liquid chamber (305) are both arranged in a circular shape; the outer diameter of the liquid chamber (305) is smaller than the inner diameter of the gas chamber (302); a rotating liquid supply component is provided at the upper end of the stirring shaft (3) in conjunction with the liquid chamber (305); several liquid outlet pipes (308) are provided on the outer wall of the stirring shaft (3); the liquid outlet pipes (308) are connected to the liquid chamber (305).
5. The microbial culture apparatus according to claim 4, wherein The rotary liquid supply assembly is positioned above the rotary gas supply assembly; the drive mechanism includes a motor (6); the motor (6) is connected to the stirring shaft (3) via a transmission assembly; the transmission assembly is positioned between the rotary liquid supply assembly and the rotary gas supply assembly.
6. The microbial culture apparatus according to claim 5, wherein The rotary liquid supply assembly includes a threaded hole (306) fixedly installed at the top of the stirring shaft (3); a rotary joint (7) is threaded inside the threaded hole (306); the threaded hole (306) and the liquid chamber (305) are connected through a connecting hole (307).
7. The microbial culture apparatus according to claim 5, wherein The liquid outlet pipe (308) and the stirring rod (301) are staggered.
8. The microbial culture apparatus of claim 5, wherein The transmission assembly includes a driven pulley (8) that is keyed to the stirring shaft (3); the driven pulley (8) is connected to a driving pulley (10) via a synchronous belt (9); the driving pulley (10) is keyed to the output shaft of the motor (6); the motor (6) is mounted on the upper cover (2).