Filtering type enrichment sampling bag for water pathogen detection

Abstract

The utility model discloses a filtering type enrichment sampling bag for water pathogen detection. The sampling bag comprises a sampling bag main body, a water inlet filter membrane assembly and a water outlet filter membrane assembly, the sampling bag main body is provided with a water inlet and a water outlet; the water inlet filter membrane assembly is detachably mounted on the water inlet and comprises a water inlet filter membrane and a water inlet sealing cover, the water inlet filter membrane is arranged between the water inlet sealing cover and the water inlet, and the water inlet sealing cover is used for sealing the water inlet; the water outlet filter membrane component is detachably mounted on the water outlet, the water outlet filter membrane component sequentially comprises a plurality of water outlet filter membrane groups and a water outlet sealing cover from one side in contact with the sampling bag main body to the outside, the pore diameters of filter membranes of the water outlet filter membrane groups are gradually reduced, and the water outlet sealing cover is used for sealing the water outlet. According to the sampling bag, large-particle impurities in a water body can be effectively filtered, pathogens of different sizes can be directly filtered and intercepted through the water outlet filter membrane assembly, operation is easy and convenient, errors and pollution caused by repeated water body transfer are avoided, and a foundation is laid for accurate detection of the pathogens in the water body.

Description

Technical Field

[0001] This application relates to the field of water sampling technology, and in particular to a filter-type enrichment sampling bag for detecting pathogens in water. Background Technology

[0002] In water body monitoring, accurate detection of pathogens such as parasites, bacteria, and viruses is crucial for ensuring water safety. However, existing water sampling bags are typically simple in design, primarily used for collecting water samples. Subsequent testing and analysis require additional steps, and they suffer from the following defects and shortcomings:

[0003] 1. Water samples collected using traditional sampling bags may contain large particles of impurities, such as silt, which can affect subsequent experimental analysis and processing.

[0004] 2. Traditional sampling bags require complex filtration after collecting water samples, which is not only inconvenient to use, but also increases the possibility of water sample processing and detection errors.

[0005] 3. Traditional sampling bags require multiple transfers and operations of water samples during laboratory processing and analysis, which increases the probability of sample contamination and can easily cause cross-contamination of the testing environment and other reagents or samples, thus affecting the accuracy of the test results. Summary of the Invention

[0006] The purpose of this application is to provide an improved filter-type enrichment sampling bag for the detection of pathogens in water bodies.

[0007] The following technical solution is adopted in this application:

[0008] This application discloses a filter-type enrichment sampling bag for pathogen detection in water, comprising a sampling bag body, an inlet filter membrane assembly, and an outlet filter membrane assembly. The sampling bag body has an inlet and an outlet. The inlet filter membrane assembly is detachably installed on the inlet and includes an inlet filter membrane and an inlet sealing cap. The inlet filter membrane is disposed between the inlet sealing cap and the inlet, and the inlet sealing cap is used to seal the inlet. The outlet filter membrane assembly is detachably installed on the outlet and includes, from the side in contact with the sampling bag body outwards, a plurality of outlet filter membrane groups with gradually decreasing pore sizes and an outlet sealing cap. The outlet sealing cap is used to seal the outlet. The inlet filter membrane is used to filter and remove large particulate impurities from the water, while the outlet filter membrane groups with gradually decreasing pore sizes are used to filter and retain pathogens of different sizes. The pore size can be set according to the pathogens to be filtered and retained as needed.

[0009] It should be noted that the filter-type enrichment sampling bag for water pathogen detection in this application uses an inlet filter membrane assembly to filter large particulate impurities in the water, and then uses an outlet filter membrane assembly to filter pathogens of different sizes. This can effectively filter and obtain pathogens in the water, thus facilitating accurate detection of parasites, bacteria, viruses and other pathogens in the water.

[0010] In one implementation of this application, the pore size of the influent filter membrane is 100-150 μm.

[0011] In one implementation of this application, the inlet filter membrane is a nylon filter screen.

[0012] It should be noted that the main function of the influent filter membrane is to filter and remove large particulate impurities and other solid impurities larger than large particulate impurities. Furthermore, it cannot prevent pathogens such as parasites, bacteria, and viruses from entering the sampling bag. Therefore, a pore size of 100-150 μm is generally sufficient for most applications. Understandably, if the pore size of the influent filter membrane is too small, it may easily filter out some larger parasites; if the pore size is too large, it may fail to filter out large particulate impurities, or allow too many large particulate impurities to enter the sampling bag, affecting the subsequent enrichment and detection of pathogens.

[0013] In one implementation of this application, the pore size of the filter membrane in the effluent filter membrane group is 0.2-10 μm.

[0014] In one implementation of this application, the effluent filter membrane group consists of a first effluent filter membrane, a second effluent filter membrane, and a third effluent filter membrane; the first effluent filter membrane is used to filter and intercept parasites, the second effluent filter membrane is used to filter and intercept bacteria, and the third effluent filter membrane is used to filter and intercept viruses.

[0015] It should be noted that the first to third effluent membranes are only one implementation of this application designed to intercept conventional parasites, bacteria, and viruses in the water. It is understood that, depending on the objects to be intercepted and their size, more effluent membrane groups of different sizes can be designed, not limited to three, nor limited to membrane pore sizes of 0.2-10μm.

[0016] In one implementation of this application, the pore size of the first effluent filter membrane is 8-10 μm, the pore size of the second effluent filter membrane is 0.4-0.45 μm, and the pore size of the third effluent filter membrane is 0.2-0.22 μm.

[0017] In one implementation of this application, the first effluent filter membrane is a polycarbonate membrane, the second effluent filter membrane is a nitrocellulose membrane, and the third effluent filter membrane is a nylon membrane.

[0018] In one implementation of this application, the inlet is located at the top of the sampling bag body, and the outlet is located at the bottom of the sampling bag body.

[0019] In one implementation of this application, the capacity of the sampling bag body is 500-1000mL.

[0020] In one implementation of this application, the sampling bag body is marked with graduations.

[0021] The beneficial effects of this application are as follows:

[0022] The water pathogen filtration enrichment sampling bag of this application can effectively filter large particulate impurities in water and directly filter and intercept pathogens of different sizes through the effluent filter membrane assembly. It does not require multiple transfers of water samples, is simple and convenient to operate, and avoids errors and pollution caused by multiple water transfers, laying the foundation for accurate detection of pathogens in water. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the structure of the water pathogen filtration enrichment sampling bag in the embodiments of this application;

[0024] Figure 2 This is a schematic diagram of the structure of the inlet filter membrane assembly of the water pathogen filtration enrichment sampling bag in the embodiments of this application;

[0025] Figure 3 This is a schematic diagram of the effluent filter membrane assembly of the water pathogen filtration enrichment sampling bag in the embodiments of this application. Detailed Implementation

[0026] The present application will be further described in detail below with reference to specific embodiments and accompanying drawings. The following embodiments are only for further illustration of the present application and should not be construed as limiting the present application.

[0027] Example

[0028] This example uses a water pathogen filtration enrichment sampling bag, such as... Figures 1 to 3 As shown, the sampling bag includes a main body 1, an inlet filter membrane assembly 2, and an outlet filter membrane assembly 3. The main body 1 has an inlet 11 and an outlet 12. The inlet filter membrane assembly 2 is detachably installed on the inlet 11 and includes an inlet filter membrane 21 and an inlet sealing cap 22. Figure 2 As shown, the inlet filter membrane 21 is disposed between the inlet sealing cap 22 and the inlet 11, and the inlet sealing cap 22 is used to seal the inlet 11; the outlet filter membrane assembly 3 is detachably installed on the outlet 12, as shown. Figure 3As shown, the water outlet filter membrane assembly 3 includes, from the side in contact with the sampling bag body 1, a number of water outlet filter membrane groups 31 with gradually decreasing filter membrane pore size and a water outlet sealing cap 32, which are used to seal the water outlet 12.

[0029] Specifically, in this example, the water effluent filter membrane assembly 31 consists of a first water effluent filter membrane 311, a second water effluent filter membrane 312, and a third water effluent filter membrane 313, as follows: Figure 3 As shown, the first effluent filter membrane 311 is used to filter and intercept parasites, the second effluent filter membrane 312 is used to filter and intercept bacteria, and the third effluent filter membrane 313 is used to filter and intercept viruses.

[0030] In this example, the inlet filter membrane 21 has a pore size of 100-150 μm, specifically a nylon filter with a pore size of 100 μm. The outlet filter membrane assembly 31 has a pore size of 0.2-10 μm. In this example, the first outlet filter membrane 311 uses a polycarbonate membrane with a pore size of 8-10 μm, the second outlet filter membrane 312 uses a nitrocellulose membrane with a pore size of 0.4-0.45 μm, and the third outlet filter membrane 313 uses a nylon membrane with a pore size of 0.2-0.22 μm. Specifically, the first outlet filter membrane 311 uses a 10 μm polycarbonate membrane, which can effectively intercept parasites; the second outlet filter membrane 312 uses a 0.45 μm nitrocellulose membrane, which can effectively intercept bacteria; and the third outlet filter membrane 313 uses a 0.22 μm nylon membrane, which can effectively intercept viruses.

[0031] The sampling bag in this example, such as Figure 1 As shown, the inlet 11 is located at the top of the sampling bag body 1, and the outlet 12 is located at the bottom of the sampling bag body 1. The capacity of the sampling bag body 1 can be designed according to usage requirements, generally 500-1000mL, and in this example, it is 1000mL; in addition, in order to facilitate observation of the water volume in the sampling bag, a scale is marked on the sampling bag body 1.

[0032] This example of a sampling bag with filtration function features a detachable cap design for both the inlet filter membrane assembly 2 and the outlet filter membrane assembly 3. The internal filter membranes, such as the inlet filter membrane 21, the first outlet filter membrane 311, the second outlet filter membrane 312, and the third outlet filter membrane 313, are all replaceable. The large-pore filter membrane at the inlet end intercepts large particles such as sediment; the small-pore filter membrane at the outlet end traps viruses, bacteria, and parasites. This sampling bag has a simple structure, convenient filter membrane replacement, adaptability to different water quality environments, and is easy to carry and maintain, making it suitable for field sampling or waterborne pathogen enrichment scenarios.

[0033] In one implementation of this example, the retaining ring of the detachable inlet filter membrane 21 is designed with internal threads on both sides, the protruding part of the inlet 11 is provided with external threads that match the internal threads, and the inlet sealing cover 22 is provided with external threads that match the internal threads near the protruding part of the detachable inlet filter membrane 21, thereby achieving detachable installation. Figure 2 As shown.

[0034] Similarly, the first outlet filter membrane 311, the second outlet filter membrane 312, the third outlet filter membrane 313, and the outlet sealing cap 32 also employ internal and external thread designs to achieve detachable installation, such as... Figure 3 As shown, the retaining ring of the first outlet filter membrane 311 has an internal thread on the side that contacts the outlet 12, and the corresponding protruding part of the outlet 12 has a matching external thread. The other side of the retaining ring of the first outlet filter membrane 311 has an external thread. The retaining ring of the second outlet filter membrane 312 has an internal thread on the side connected to the first outlet filter membrane 311, matching the external thread of the first outlet filter membrane 311. The other side of the retaining ring of the second outlet filter membrane 312 has an external thread. The third outlet filter membrane 313 has a similar structure to the second outlet filter membrane 312. It can be understood that, in principle, the outlet filter membranes that can be connected are not limited to the first outlet filter membrane 311, the second outlet filter membrane 312, and the third outlet filter membrane 313. The number of outlet filter membranes can be increased or decreased according to the pathogens that need to be filtered and intercepted. Finally, the outlet sealing cap 32 is designed with an internal thread that matches the external thread of the third outlet filter membrane 313, thereby closing the outlet 11. The raised external threads of the first effluent filter membrane 311, the second effluent filter membrane 312, and the third effluent filter membrane 313 can be adapted to a vacuum pump for sampling or vacuum filtration.

[0035] The above description, in conjunction with specific embodiments, provides a further detailed explanation of this application and should not be construed as limiting the specific implementation of this application to these descriptions. Those skilled in the art to which this application pertains can make several simple deductions or substitutions without departing from the concept of this application.

Claims

1. A filter-type enrichment sampling bag for detecting pathogens in water, characterized in that: It includes a sampling bag body (1), an inlet filter membrane assembly (2), and an outlet filter membrane assembly (3); The main body (1) of the sampling bag is provided with an inlet (11) and an outlet (12); The water inlet filter membrane assembly (2) is detachably installed on the water inlet (11). The water inlet filter membrane assembly (2) includes a water inlet filter membrane (21) and a water inlet sealing cover (22). The water inlet filter membrane (21) is disposed between the water inlet sealing cover (22) and the water inlet (11). The water inlet sealing cover (22) is used to close the water inlet (11). The water outlet filter membrane assembly (3) is detachably installed on the water outlet (12). The water outlet filter membrane assembly (3) includes several water outlet filter membrane groups (31) with gradually decreasing filter membrane pore size and a water outlet sealing cap (32) from the side in contact with the sampling bag body (1) outward. The water outlet sealing cap (32) is used to seal the water outlet (12).

2. The sampling bag according to claim 1, characterized in that: The filter membrane (21) has a pore size of 100-150 μm.

3. The sampling bag according to claim 2, characterized in that: The inlet filter membrane (21) is a nylon filter screen.

4. The sampling bag according to claim 1, characterized in that: The pore size of the effluent filter membrane group (31) is 0.2-10μm.

5. The sampling bag according to claim 1, characterized in that: The water effluent filter membrane group (31) consists of a first water effluent filter membrane (311), a second water effluent filter membrane (312), and a third water effluent filter membrane (313); the first water effluent filter membrane (311) is used to filter and intercept parasites, the second water effluent filter membrane (312) is used to filter and intercept bacteria, and the third water effluent filter membrane (313) is used to filter and intercept viruses.

6. The sampling bag according to claim 5, characterized in that: The first effluent filter membrane (311) has a pore size of 8-10 μm, the second effluent filter membrane (312) has a pore size of 0.4-0.45 μm, and the third effluent filter membrane (313) has a pore size of 0.2-0.22 μm.

7. The sampling bag according to claim 5, characterized in that: The first water effluent filter membrane (311) is a polycarbonate membrane, the second water effluent filter membrane (312) is a nitrocellulose membrane, and the third water effluent filter membrane (313) is a nylon membrane.

8. The sampling bag according to any one of claims 1-7, characterized in that: The inlet (11) is located at the top of the sampling bag body (1), and the outlet (12) is located at the bottom of the sampling bag body (1).

9. The sampling bag according to any one of claims 1-7, characterized in that: The sampling bag body (1) has a capacity of 500-1000mL.

10. The sampling bag according to claim 9, characterized in that: The sampling bag body (1) is marked with graduations.

Images