Kit for detecting avian influenza and chicken parvovirus

Abstract

The utility model provides an avian influenza and chicken parvovirus detection kit, which belongs to the field of poultry breeding and comprises a detection kit body, and a detection mechanism for detecting avian influenza and chicken parvovirus is arranged in the detection kit body. A sealing assembly for improving the sealing performance of the detection kit body is arranged at the top of the detection kit body. According to the detection mechanism, when the kit is used, the sample collecting tube is used for collecting poultry samples, the avian influenza virus detection reagent bottle contains specific primers and probes and can identify nucleic acid of avian influenza viruses, and the chicken parvovirus detection reagent bottle contains specific primers and probes and can identify nucleic acid of chicken parvoviruses and can identify nucleic acid of chicken parvoviruses. The extraction reagent bottle is used for extracting viral nucleic acid from a sample, the porous plate is used for placing the extracted sample and PCR reaction liquid and carrying out nucleic acid amplification detection, and the device is simple to operate, short in time consumption, low in cost and convenient to use by workers.

Description

Technical Field

[0001] This utility model relates to the field of poultry farming, and more specifically, to a detection kit for avian influenza and chicken parvovirus. Background Technology

[0002] Avian influenza and chicken parvovirus are important pathogens affecting poultry health. Avian influenza is an infectious disease caused by the avian influenza virus, which can cause significant losses to poultry and related economic sectors, and even threaten public health security. Chicken parvovirus is a DNA virus that mainly affects the growth and development of chickens, leading to symptoms such as diarrhea, anemia, and immunosuppression. In severe cases, it can cause large-scale mortality in flocks. Currently, there are still the following shortcomings in the detection of avian influenza and chicken parvovirus:

[0003] Currently, diagnostic methods for avian influenza and chicken parvovirus mainly rely on traditional methods such as virus isolation, virus culture, and serological detection. While these methods are highly accurate, they require specialized laboratory equipment and are complex, time-consuming, and costly. Therefore, this paper proposes a detection kit for avian influenza and chicken parvovirus. Utility Model Content

[0004] The purpose of this invention is to address the problem that current diagnostic methods for avian influenza and chicken parvovirus mainly rely on traditional methods such as virus isolation, virus culture, and serological detection. While these methods are highly accurate, they require specialized experimental equipment and are complex, time-consuming, and costly to operate.

[0005] To achieve the above-mentioned objectives, this utility model provides the following technical solution:

[0006] The present invention is as follows: A detection kit for avian influenza and chicken parvovirus, comprising a detection kit body, wherein the detection kit body is provided with a detection mechanism for detecting avian influenza and chicken parvovirus inside, and a sealing component for improving the sealing performance of the detection kit body is provided on the top of the detection kit body.

[0007] The detection mechanism includes two sample collection tubes disposed inside the main body of the detection kit. One end of each sample collection tube is threaded with a sealing cap. Inside the main body of the detection kit, there are avian influenza virus detection reagent bottles, chicken parvovirus detection reagent bottles, and extraction reagent bottles, respectively. The tops of the avian influenza virus detection reagent bottles, chicken parvovirus detection reagent bottles, and extraction reagent bottles are all threaded with threaded caps. A multi-well plate is disposed on one side of each sample collection tube. Positive control samples and negative control samples are respectively adhered to the inner bottom wall of the main body of the detection kit.

[0008] As a preferred technical solution of this utility model, the sealing assembly includes a cover plate hinged to the top of the test kit body, an arc-shaped block fixedly connected to the outer wall of both the cover plate and the test kit body, a protruding plate fixedly connected to the opposite side wall of the arc-shaped block, a fixing ring slidably connected to the outside of the arc-shaped block, and a groove formed on the inner wall of the fixing ring.

[0009] As a preferred technical solution of this utility model, a fixing plate is fixedly connected to the inner bottom wall of the test kit body, and the avian influenza virus test reagent bottle, chicken parvovirus test reagent bottle and extraction reagent bottle are all inserted into the fixing plate.

[0010] As a preferred technical solution of this utility model, a convex groove is provided on the top of the test kit body, a positioning plate is slidably connected inside the convex groove, and a pressure plate is fixedly connected to the side wall of the cover plate, and the pressure plate is slidably connected to the convex groove.

[0011] As a preferred technical solution of this utility model, a vertical plate is fixedly connected to the inner bottom wall of the test kit body, and a triangular plate is fixedly connected to the side wall of the vertical plate near the multi-well plate. There are four vertical plates and four triangular plates, which are distributed in pairs on opposite sides of the multi-well plate. The top of the multi-well plate and the bottom of the triangular plate are tightly fitted together.

[0012] As a preferred technical solution of this utility model, two C-shaped parts are fixedly connected to the inner bottom wall of the test kit body, and the two sample collection tubes are snapped into the two C-shaped parts. The two C-shaped parts are made of plastic.

[0013] As a preferred technical solution of this utility model, a storage frame is provided on the inner bottom wall of the test kit body.

[0014] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0015] 1. The device utilizes a set-up detection mechanism. Sample collection tubes are used to collect poultry samples. Avian influenza virus detection reagent bottles contain specific primers and probes to identify the nucleic acid of avian influenza virus. Chicken parvovirus detection reagent bottles contain specific primers and probes to identify the nucleic acid of chicken parvovirus. Extraction reagent bottles are used to extract viral nucleic acid from the samples. Multiwell plates are used to hold the extracted samples and PCR reaction solution for nucleic acid amplification detection. Positive control samples provide known positive samples of avian influenza or chicken parvovirus to verify the effectiveness of the kit. Negative control samples provide known negative samples to ensure that virus-free samples do not produce false positives. The device is simple to operate, time-efficient, and low-cost, making it easy for staff to use.

[0016] 2. With the sealing assembly in place, rotate the cover plate during use to make the side wall of the cover plate fit against the top of the test kit body. Then, let the groove pass through the convex plate until the side wall of the fixing ring fits against the side wall of the test kit body. At this point, rotate the fixing ring 90 degrees to quickly fix the two arc-shaped blocks, preventing the cover plate from rotating accidentally and ensuring that the internal container of the test kit body is not damaged. Attached Figure Description

[0017] Figure 1 A schematic diagram of the structure of the avian influenza and chicken parvovirus detection kit provided by this utility model;

[0018] Figure 2 Left view of the avian influenza and chicken parvovirus detection kit provided by this utility model;

[0019] Figure 3 The avian influenza and chicken parvovirus detection kit provided by this utility model Figure 2 A three-dimensional cross-sectional view at point AA;

[0020] Figure 4 A partial structural schematic diagram of the detection mechanism of the avian influenza and chicken parvovirus detection kit provided by this utility model;

[0021] Figure 5 A partial structural schematic diagram of the sealing component of the avian influenza and chicken parvovirus detection kit provided by this utility model;

[0022] Figure 6 This is a schematic diagram of the vertical plate and triangular plate structure of the avian influenza and chicken parvovirus detection kit provided by this utility model.

[0023] The diagram shows: 1. Test kit body; 2. Detection mechanism; 3. Sealing component; 4. Fixing plate; 5. Convex groove; 6. Positioning plate; 7. Pressure plate; 8. Vertical plate; 9. Triangular plate; 10. C-shaped part; 11. Storage frame; 201. Sample collection tube; 202. Sealing cap; 203. Avian influenza virus test kit bottle; 204. Chicken parvovirus test kit bottle; 205. Extraction reagent bottle; 206. Threaded cap; 207. Multi-well plate; 208. Positive control sample; 209. Negative control sample; 301. Cover plate; 302. Arc-shaped block; 303. Convex plate; 304. Fixing ring; 305. Groove. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model.

[0025] Therefore, the following detailed description of the embodiments of this utility model is not intended to limit the scope of the claimed utility model, but merely to illustrate some embodiments of the utility model. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without inventive effort are within the scope of protection of this utility model.

[0026] It should be noted that, unless otherwise specified, the embodiments and features and technical solutions in the present invention can be combined with each other.

[0027] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0028] like Figure 1 As shown, this embodiment proposes an avian influenza and chicken parvovirus detection kit, including a detection kit body 1, a detection mechanism 2 for detecting avian influenza and chicken parvovirus is provided inside the detection kit body 1, and a sealing component 3 for improving the sealing of the detection kit body 1 is provided on the top of the detection kit body 1.

[0029] like Figure 3 and Figure 4As shown, the detection mechanism 2 includes two sample collection tubes 201 disposed inside the main body 1 of the detection kit. Both sample collection tubes 201 contain buffer solution, and one end of each sample collection tube 201 is threadedly connected to a sealing cap 202. The main body 1 of the detection kit contains an avian influenza virus detection reagent bottle 203, a chicken parvovirus detection reagent bottle 204, and an extraction reagent bottle 205. The avian influenza virus detection reagent bottle 203 contains extraction reagents, amplification reagents (PCR primers, fluorescent probes), and internal control primers. The chicken parvovirus detection reagent bottle 204 contains extraction reagents, amplification reagents (PCR primers, fluorescent probes), and internal control primers. The extraction reagent bottle 205 contains DNA / RNA extraction reagents. The tops of the avian influenza virus detection reagent bottle 203, chicken parvovirus detection reagent bottle 204, and extraction reagent bottle 205 are threadedly connected to threaded caps 206. A multi-well plate 207 is disposed on one side of the sample collection tubes 201. Positive control samples 2 are respectively adhered to the inner bottom wall of the main body 1. For samples 08 and 209 (negative control), the avian blood sample is placed in sample collection tube 201, immersing it in the buffer solution. Extraction reagent bottle 205 is opened, and the sample is transferred from sample collection tube 201 to extraction reagent bottle 205. Then, using an external extraction tool, viral nucleic acid is extracted from the sample. The extracted nucleic acid is transferred to the wells of multi-well plate 207. Liquids from avian influenza virus detection reagent bottle 203 and chicken parvovirus detection reagent bottle 204 are added. These reagents contain specific primers and probes and can be used to detect the nucleic acid of avian influenza virus and chicken parvovirus. If the sample contains avian influenza virus or chicken parvovirus, the PCR reaction will show a fluorescent signal, indicating the presence of the target nucleic acid. Samples without the target virus will not produce a fluorescent signal, indicating a negative result. Positive control sample 208 is used to verify the experimental validity; positive control sample 208 successfully detects the nucleic acid of the target virus. Negative control sample 209 is used to verify the specificity of the experiment, ensuring that virus-free samples will not show false positive results.

[0030] like Figure 4 and Figure 5As shown, the sealing assembly 3 includes a cover plate 301 hinged to the top of the test kit body 1. Arc-shaped blocks 302 are fixedly connected to both the cover plate 301 and the outer wall of the test kit body 1. Protruding plates 303 are fixedly connected to the opposite sidewalls of the arc-shaped blocks 302. A retaining ring 304 is slidably connected to the outside of the arc-shaped blocks 302. A groove 305 is formed on the inner wall of the retaining ring 304. During use, the cover plate 301 is rotated to make its sidewalls fit against the top of the test kit body 1. Then... Align the groove 305 with the protrusion 303 and move the fixing ring 304 until the side wall of the fixing ring 304 is in contact with the outer wall of the test kit body 1. Then rotate the fixing ring 304 ninety degrees to fix the two arc blocks 302, preventing the cover plate 301 from being opened and exposing the test mechanism 2 to the environment. When opening the cover plate 301, rotate the fixing ring 304 ninety degrees in the opposite direction and then move the fixing ring 304 away from the test kit body 1 so that the groove 305 passes through the protrusion 303.

[0031] like Figure 3 As shown, a fixing plate 4 is fixedly connected to the inner bottom wall of the test kit body 1. The avian influenza virus test kit bottle 203, the chicken parvovirus test kit bottle 204, and the extraction reagent bottle 205 are all inserted into the fixing plate 4. The fixing plate 4 can limit the avian influenza virus test kit bottle 203, the chicken parvovirus test kit bottle 204, and the extraction reagent bottle 205 to prevent them from moving inside the test kit body 1.

[0032] like Figure 1 As shown, a convex groove 5 is provided on the top of the test kit body 1. A positioning plate 6 is slidably connected inside the convex groove 5. A pressure plate 7 is fixedly connected to the side wall of the cover plate 301. The pressure plate 7 is slidably connected to the convex groove 5. When the side wall of the cover plate 301 is in contact with the top of the test kit body 1, the pressure plate 7 will be in contact with the top of the positioning plate 6, thereby fixing the positioning plate 6. At this time, the positioning plate 6 can fix the threaded cap 206 on the avian influenza virus test kit bottle 203, the chicken parvovirus test kit bottle 204, and the extraction reagent bottle 205, preventing the avian influenza virus test kit bottle 203, the chicken parvovirus test kit bottle 204, and the extraction reagent bottle 205 from moving up and down inside the test kit body 1.

[0033] like Figure 6As shown, a vertical plate 8 is fixedly connected to the inner bottom wall of the test kit body 1. A triangular plate 9 is fixedly connected to the side wall of the vertical plate 8 near the multi-well plate 207. There are four vertical plates 8 and four triangular plates 9, which are distributed in pairs on opposite sides of the multi-well plate 207. The top of the multi-well plate 207 is in close contact with the bottom of the triangular plate 9. When installing the multi-well plate 207, the side wall of the multi-well plate 207 is made to contact the inclined side of the triangular plate 9, and the multi-well plate 207 is pressed down. At this time, the vertical plate 8 bends until the bottom of the multi-well plate 207 is in contact with the bottom wall of the test kit body 1. At this time, the top of the multi-well plate 207 is in contact with the bottom of the triangular plate 9, thereby fixing the multi-well plate 207. When disassembling the multi-well plate 207, the vertical plate 8 is bent to the side away from the multi-well plate 207, and then the multi-well plate 207 is lifted up to quickly disassemble it.

[0034] like Figure 1 As shown, two C-shaped parts 10 are fixedly connected to the inner bottom wall of the test kit body 1. Two sample collection tubes 201 are snapped into the two C-shaped parts 10. The two C-shaped parts 10 are made of plastic. When in use, the sample collection tubes 201 are snapped into the inside of the C-shaped parts 10 to prevent the sample collection tubes 201 from moving inside the test kit body 1 and causing damage.

[0035] like Figure 1 As shown, a storage frame 11 is provided on the inner bottom wall of the test kit body 1. When in use, the storage frame 11 can store the instruction manuals for the avian influenza virus test kit bottle 203, the chicken parvovirus test kit bottle 204, and the extraction reagent bottle 205.

[0036] Specifically, when using this avian influenza and chicken parvovirus detection kit: place the poultry blood sample in the sample collection tube 201, immersing it in the buffer solution, open the extraction reagent bottle 205, and transfer the sample from the sample collection tube 201 to the extraction reagent bottle 205 (e.g., Figure 3 (As shown), then using an external extraction tool, viral nucleic acid is extracted from the sample. The extracted nucleic acid is transferred to the wells of multi-well plate 207, and the liquids from avian influenza virus detection reagent bottle 203 and chicken parvovirus detection reagent bottle 204 are added. These reagents contain specific primers and probes and can be used to detect the nucleic acid of avian influenza virus and chicken parvovirus. If the sample contains avian influenza virus or chicken parvovirus, the PCR reaction will show a fluorescent signal, indicating the presence of the target nucleic acid. Samples without the target virus will not produce a fluorescent signal, indicating that the sample is negative (e.g., ...). Figure 3 and Figure 4 As shown), positive control sample 208 was used to verify the effectiveness of the experiment. Positive control sample 208 successfully detected the nucleic acid of the target virus. Negative control sample 209 was used to verify the specificity of the experiment, ensuring that virus-free samples would not show false positive results (e.g., Figure 4As shown in the figure, the test can be easily completed by following the above steps.

[0037] All technical features in this embodiment can be freely combined according to actual needs.

[0038] The above embodiments are preferred implementations of this utility model. In addition, this utility model can also be implemented in other ways. Any obvious substitutions without departing from the concept of this technical solution are within the protection scope of this utility model.

Claims

1. A detection kit for avian influenza and chicken parvovirus, comprising a detection kit body (1), characterized in that, The test kit body (1) is equipped with a detection mechanism (2) for detecting avian influenza and chicken parvovirus, and a sealing component (3) for improving the sealing of the test kit body (1) is provided on the top of the test kit body (1). The detection mechanism (2) includes two sample collection tubes (201) disposed inside the detection kit body (1). One end of each of the two sample collection tubes (201) is threaded with a sealing cap (202). The detection kit body (1) is respectively provided with an avian influenza virus detection reagent bottle (203), a chicken parvovirus detection reagent bottle (204) and an extraction reagent bottle (205). The top of each of the avian influenza virus detection reagent bottle (203), chicken parvovirus detection reagent bottle (204) and extraction reagent bottle (205) is threaded with a threaded cap (206). A multi-well plate (207) is provided on one side of the sample collection tube (201). A positive control sample (208) and a negative control sample (209) are respectively adhered to the inner bottom wall of the detection kit body (1).

2. The avian influenza and chicken parvovirus detection kit according to claim 1, characterized in that, The sealing assembly (3) includes a cover plate (301) hinged to the top of the test kit body (1). An arc-shaped block (302) is fixedly connected to both the cover plate (301) and the outer wall of the test kit body (1). A protruding plate (303) is fixedly connected to the opposite side wall of the arc-shaped block (302). A fixing ring (304) is slidably connected to the outside of the arc-shaped block (302). A groove (305) is provided on the inner wall of the fixing ring (304).

3. The avian influenza and chicken parvovirus detection kit according to claim 1, characterized in that, A fixing plate (4) is fixedly connected to the inner bottom wall of the test kit body (1), and the avian influenza virus test kit bottle (203), chicken parvovirus test kit bottle (204) and extraction reagent bottle (205) are all inserted into the fixing plate (4).

4. The avian influenza and chicken parvovirus detection kit according to claim 2, characterized in that, The top of the test kit body (1) is provided with a convex groove (5), and a positioning plate (6) is slidably connected inside the convex groove (5). A pressure plate (7) is fixedly connected to the side wall of the cover plate (301), and the pressure plate (7) is slidably connected to the convex groove (5).

5. The avian influenza and chicken parvovirus detection kit according to claim 1, characterized in that, A vertical plate (8) is fixedly connected to the inner bottom wall of the test kit body (1). A triangular plate (9) is fixedly connected to the side wall of the vertical plate (8) near the multi-well plate (207). There are four vertical plates (8) and triangular plates (9), which are distributed in pairs on opposite sides of the multi-well plate (207). The top of the multi-well plate (207) is in close contact with the bottom of the triangular plate (9).

6. The avian influenza and chicken parvovirus detection kit according to claim 1, characterized in that, Two C-shaped parts (10) are fixedly connected to the inner bottom wall of the test kit body (1), and the two sample collection tubes (201) are snapped into the two C-shaped parts (10). The two C-shaped parts (10) are made of plastic.

7. The avian influenza and chicken parvovirus detection kit according to claim 1, characterized in that, A storage frame (11) is provided on the inner bottom wall of the test kit body (1).

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