Pipettor for cell culture

Abstract

The utility model provides a pipettor for cell culture, and relates to the technical field of liquid measuring experimental instruments. A plurality of disinfection cavities are formed in the shell; the transferring mechanism is used for transferring the pipette between the interior and the exterior of the disinfection cavity, and door plates which can be opened during transferring and closed after transferring are arranged on the side walls of the disinfection cavity of the shell respectively; the disinfection part is arranged in the disinfection cavity. When a certain external pipette needs to be fed into the disinfection cavity or a certain pipette in the disinfection cavity needs to be taken out, the pipette is transferred between the inside and the outside of the disinfection cavity through the transfer mechanism, only the door plate on the side wall of a certain disinfection cavity needs to be opened during transfer, and the door plates of other disinfection cavities are still in a closed state; therefore, normal disinfection of the pipettes in other disinfection cavities cannot be affected, the disinfection efficiency and effect can be improved, and the pollution risk of the pipettes in the disinfection process is reduced.

Description

Technical Field

[0001] This application relates to the field of liquid volumetric experimental instruments, and in particular to a pipette for cell culture. Background Technology

[0002] A pipette is a laboratory instrument used to accurately measure and transfer small or micro amounts of liquid. By controlling the movement of the piston or air cushion, a pipette can achieve precise control of the liquid volume (the range is usually from 0.1 μL to 10 mL). Pipettes are widely used in molecular biology, medical testing and other fields, and are often used in scenarios that require quantitative operations, such as solution preparation, sample dispensing and reagent addition.

[0003] Pipettes, including the pipette tip, may come into contact with lab surfaces, operator hands, airborne dust, or other non-sterile objects. Contaminants from these non-sterile objects may come into contact with the sample during pipetting or diffuse as aerosols into reagent bottles, petri dishes, etc., leading to inaccurate experimental results. Therefore, pipettes need to be sterilized. Common sterilization methods include alcohol sterilization, ultraviolet sterilization, and ozone sterilization. For example, utility model patent CN222657497U discloses a pipette sterilization device, including a housing. The housing cavity is divided into an ozone generating chamber and multiple pipette chambers, each connected to the ozone generating chamber. The housing is a box, including a body and a hinged lid. The upper surface of the body has entrances to the pipette chambers.

[0004] When using the aforementioned pipette sterilization device to sterilize pipettes, the lid is first opened, the pipette is placed in the pipette chamber of the device, and then the lid is closed. Ozone is generated in the ozone generator chamber and enters the pipette chamber to sterilize the pipette. However, when the lid of the sterilization device is open, the entrance to the pipette chamber is completely exposed to the air. In actual use, pipettes are usually placed in or removed from the pipette chamber one at a time and in a random manner. Therefore, when a single pipette is placed in or removed from the device, other pipettes being sterilized in the chambers are exposed to the air, affecting the sterilization of other pipettes. This not only reduces sterilization efficiency but also increases the risk of contamination. Utility Model Content

[0005] The purpose of this application is to provide a pipette for cell culture, which solves the problem in related technologies where opening the lid of a pipette sterilization device to put in or take out a single pipette can affect other pipettes that are being sterilized, thereby reducing sterilization efficiency and increasing the risk of contamination.

[0006] The pipette for cell culture provided in this application adopts the following technical solution:

[0007] A pipette for cell culture, comprising:

[0008] pipette;

[0009] A housing, wherein the housing contains multiple disinfection chambers;

[0010] A transfer mechanism is provided for transferring the pipette between the inside and outside of the disinfection chamber. The side walls of the shell disinfection chamber are respectively provided with door panels that can be opened during transfer and closed after transfer.

[0011] Positioning components are respectively disposed in the disinfection chamber and are used to position the pipette transferred to the disinfection chamber by the transfer mechanism;

[0012] A disinfection component is disposed within the disinfection chamber.

[0013] Optionally, the transfer mechanism includes a rotary drive, a support, and a push-pull frame. The rotary drive is fixed to the housing, the support is connected to the rotary drive, and the push-pull frame is telescopically mounted on the support. The support is provided with a telescopic drive for driving the push-pull frame to extend or retract relative to the support. The push-pull frame is used to support the pipette, and the push-pull frame is provided with a first limiting part for limiting the position of the pipette.

[0014] Optionally, it also includes an opening and closing mechanism, which is disposed on the housing. The push-pull bracket can act on the opening and closing mechanism and drive the door panel to open through the opening and closing mechanism. When the push-pull bracket is separated from the opening and closing mechanism, the opening and closing mechanism can drive the door panel to close.

[0015] Optionally, the opening and closing mechanism includes a first elastic element and a transmission assembly. A hinge shaft is fixedly provided on the door panel, and the door panel is hinged to the housing through the hinge shaft. The transmission assembly is disposed on the housing and connected to the hinge shaft. The push-pull bracket can act on the transmission assembly and drive the hinge shaft to rotate through the transmission assembly. When the push-pull bracket is separated from the opening and closing mechanism, the first elastic element can act on the hinge shaft and drive the hinge shaft to reverse.

[0016] Optionally, a rotary drive component is also included. The disinfection component is an ultraviolet lamp. The rotary drive component is disposed inside the disinfection chamber. The rotary drive component is connected to the positioning component and is used to drive the positioning component to rotate.

[0017] Optionally, it also includes a lifting drive and a lifting frame. The lifting drive is fixed to the housing, the lifting frame is connected to the lifting drive, the rotation drive is fixed to the lifting frame, and the positioning component includes a bracket. The bracket is connected to the rotation drive and is used to support the pipette. The bracket is provided with a second limiting part for limiting the position of the pipette.

[0018] Optionally, the positioning element further includes a pressure plate and a second elastic element. The pressure plate is slidably disposed on the bracket in a vertical direction, and the second elastic element acts on the pressure plate and can push the pressure plate to press against the pipette from top to bottom, so as to restrict the vertical movement of the pipette.

[0019] Optionally, the bottom wall of the disinfection chamber of the housing is provided with a column, the upper end of which can abut against the bottom surface of the pressure plate to limit the travel of the pressure plate as it moves downward with the lifting frame.

[0020] In summary, this application includes at least the following beneficial technical effects: When sterilizing pipettes, the pipettes of this application are placed in sterilization chambers and sterilized using a sterilization device, while the sterilization chambers are isolated from the external environment by door panels. When it is necessary to insert an external pipette into the sterilization chamber or remove a pipette from the sterilization chamber, a transfer mechanism is used to transfer the pipette between the inside and outside of the sterilization chamber. During the transfer, only the door panel of one sterilization chamber needs to be opened; the doors of other sterilization chambers remain closed, thus not affecting the normal sterilization of pipettes in other sterilization chambers. This improves sterilization efficiency and effectiveness, and reduces the risk of contamination of pipettes during the sterilization process. Attached Figure Description

[0021] Figure 1 This is a first-view cross-sectional view of a pipette used for cell culture in an embodiment of this application;

[0022] Figure 2 This is a cross-sectional view from a second perspective of the pipette used for cell culture in an embodiment of this application;

[0023] Figure 3 This is a cross-sectional view from a third perspective of a pipette used for cell culture in an embodiment of this application;

[0024] Figure 4 for Figure 3 A magnified view of part C in the middle;

[0025] Figure 5 This is a cross-sectional view from a fourth perspective of the pipette used for cell culture in the embodiments of this application;

[0026] Figure 6 for Figure 5A magnified view of part D in the middle;

[0027] Figure 7 for Figure 1 A magnified view of part A in the middle;

[0028] Figure 8 for Figure 2 A magnified view of part B in the diagram.

[0029] Explanation of reference numerals in the attached figures:

[0030] 10. Housing; 11. Door panel; 111. Hinge shaft; 112. Gear; 12. Disinfection chamber; 13. Rotation chamber; 14. Guide sleeve; 15. Stop block; 16. Guide column; 17. Column; 18. Inlet / outlet; 19. Hinge seat;

[0031] 20. Pipette; 21. Horizontal plate; 211. First positioning hole; 212. Second positioning hole;

[0032] 30. Transfer mechanism; 31. Rotary drive component; 32. Support; 321. Guide rail; 33. Push-pull frame; 331. Boss; 332. First locating pin; 34. Telescopic drive component;

[0033] 40. Positioning component; 41. Bracket; 411. Second positioning pin; 412. Vertical rod; 413. Second flange; 42. Pressure plate; 43. Second spring;

[0034] 50. Opening and closing mechanism; 51. First spring; 52. Rack; 521. Column; 522. First flange; 523. Pin; 53. Swing frame; 531. Pivot; 532. Strip groove;

[0035] 60. Rotary drive component; 70. Lifting drive component; 80. Lifting frame; 90. Ultraviolet lamp. Detailed Implementation

[0036] The following is in conjunction with the appendix Figure 1 - Appendix Figure 8 This application will be described in further detail below.

[0037] This application discloses a pipette for cell culture.

[0038] A pipette for cell culture includes a housing 10, a pipette 20, a transfer mechanism 30, a positioning component 40, an opening and closing mechanism 50, a sterilization component, a rotation drive component 60, a lifting drive component 70, and a lifting frame 80.

[0039] Reference Figures 1 to 6 The housing 10 has multiple disinfection chambers 12 arranged in a ring. The disinfection component is located in the disinfection chamber 12 and is fixed to the inner wall of the housing 10. The disinfection component is an ultraviolet lamp 90.

[0040] The transfer mechanism 30 is used to transfer the pipette 20 between the inside and outside of the disinfection chamber 12. In an optional embodiment, the specific structure of the transfer mechanism 30 is as follows: The transfer mechanism 30 includes a rotary drive 31, a support 32 and a push-pull frame 33. The housing 10 is provided with a rotary chamber 13. The support 32 and the push-pull frame 33 are located in the rotary chamber 13. The housing 10 is provided with an inlet and outlet 18 for communicating between the rotary chamber 13 and the outside of the housing 10.

[0041] The rotary drive component 31 is fixed on the housing 10, and the support 32 is connected to the rotary drive component 31. The rotary drive component 31 can be a rotary drive motor, and the support 32 is fixed to the output shaft of the rotary drive motor.

[0042] The push-pull bracket 33 is telescopically mounted on the support 32. The support 32 is provided with a guide rail 321. The push-pull bracket 33 is slidably engaged with the guide rail 321. The support 32 is provided with a telescopic drive component 34 for driving the push-pull bracket 33 to extend and retract relative to the support 32. The telescopic drive component 34 can be a telescopic drive electric push rod.

[0043] The push-pull bracket 33 is used to support the pipette 20. The push-pull bracket 33 is provided with a first limiting part for limiting the pipette 20. More specifically, the first limiting part is a first positioning pin 332. The pipette 20 is provided with a horizontal plate 21 on its body. The horizontal plate 21 is provided with a first positioning hole 211. The pipette 20 can be placed on the push-pull bracket 33 through the horizontal plate 21 and is engaged with the first positioning pin 332 through the first positioning hole 211.

[0044] Reference Figure 4 , Figure 7 and Figure 8 The side walls of the sterilization chamber 12 of the housing 10 are respectively provided with door panels 11 that can be opened during transfer and closed after transfer. When the transfer mechanism 30 transfers the pipette 20, it can control the opening and closing of the door panels 11 through the opening and closing mechanism 50. More specifically, the opening and closing mechanism 50 is provided on the housing 10. The push-pull bracket 33 can act on the opening and closing mechanism 50 and drive the door panels 11 to open through the opening and closing mechanism 50. When the push-pull bracket 33 is separated from the opening and closing mechanism 50, the opening and closing mechanism 50 can drive the door panels 11 to close.

[0045] Furthermore, the opening and closing mechanism 50 includes a first elastic element and a transmission assembly. A hinge shaft 111 is fixed on the door panel 11, and the door panel 11 is hinged to the housing 10 via the hinge shaft 111. The transmission assembly is located on the housing 10 and connected to the hinge shaft 111. The push-pull bracket 33 can act on the transmission assembly and drive the hinge shaft 111 to rotate through the transmission assembly, thereby driving the door panel 11 to open. When the push-pull bracket 33 is separated from the opening and closing mechanism 50, the first elastic element can act on the hinge shaft 111 and drive the hinge shaft 111 to reverse, thereby driving the door panel 11 to close.

[0046] In an optional embodiment, the specific structure of the transmission assembly and the first elastic element is as follows: The transmission assembly includes a rack 52 and a swing arm 53. A gear 112 is provided on the hinge shaft 111. A guide sleeve 14 and a stop block 15 are provided on the housing 10. The rack 52 is slidably engaged with the guide sleeve 14 and meshes with the gear 112. A column 521 is provided on the rack 52. A first flange 522 is provided at the end of the column 521. The column 521 slidably passes through the stop block 15. The first elastic element is a first spring 51. The first spring 51 is sleeved on the outside of the column 521. The two ends of the first spring 51 abut against the stop block 15 and the first flange 522, respectively. The top wall of the rotating cavity 13 of the housing 10 is provided with a hinge seat 19. The swing frame 53 is hinged to the hinge seat 19 via a pivot 531. The swing frame 53 is provided with a strip groove 532. The rack 52 is provided with a pin 523. The pin 523 passes through the strip groove 532. The push-pull frame 33 is provided with a boss 331 that can push the swing frame 53.

[0047] Reference Figures 4 to 8 The lifting drive component 70 is fixed to the inner wall of the housing 10. The lifting drive component 70 can be a lifting drive electric push rod. The housing 10 is provided with a guide post 16. The lifting frame 80 is slidably mounted on the guide post 16 and connected to the lifting drive component 70. The rotation drive component 60 is fixed on the lifting frame 80 and located inside the disinfection chamber 12.

[0048] Positioning elements 40 are respectively disposed in the disinfection chamber 12 and are used to position the pipette 20 transferred by the transfer mechanism 30 into the disinfection chamber 12. The rotary drive element 60 is connected to the positioning elements 40 and is used to drive the positioning elements 40 to rotate. In an optional embodiment, the specific structure of the positioning element 40 and its specific connection relationship with the rotary drive element 60 are as follows: The positioning element 40 includes a bracket 41, a pressure plate 42 and a second elastic element. The bracket 41 is connected to the rotary drive element 60 and is used to support the pipette 20. The rotary drive element 60 can be a rotary drive motor. The bracket 41 is fixedly connected to the output shaft of the rotary drive motor. The bracket 41 is provided with a second limiting part for limiting the position of the pipette 20.

[0049] More specifically, the second limiting part is the second positioning pin 411, and the horizontal plate 21 is provided with a second positioning hole 212. The pipette 20 can be placed on the bracket 41 through the horizontal plate 21 and connected to the second positioning pin 411 through the second positioning hole 212.

[0050] The pressure plate 42 is slidably mounted on the bracket 41 along the vertical direction. The second elastic element acts on the pressure plate 42 and can push the pressure plate 42 to press against the pipette 20 from top to bottom, so as to restrict the vertical movement of the pipette 20.

[0051] More specifically, the second elastic element is a second spring 43. The bracket 41 is provided with an upwardly extending vertical rod 412. The pressure plate 42 is slidably sleeved on the vertical rod 412. The upper end of the vertical rod 412 is provided with a second flange 413. The second spring 43 is sleeved on the outside of the vertical rod 412. The two ends of the second spring 43 abut against the pressure plate 42 and the second flange 413 respectively. The pressure plate 42 can press against the upper surface of the horizontal plate 21.

[0052] The bottom wall of the disinfection chamber 12 of the housing 10 is provided with a column 17. The upper end of the column 17 can abut against the bottom surface of the pressure plate 42 to limit the travel of the pressure plate 42 as it moves downward with the lifting frame 80.

[0053] The implementation principle of the pipette for cell culture in this embodiment is as follows: When the pipette for cell culture in this application needs to send the pipette 20 from the external environment into the disinfection chamber 12 for disinfection, the support 32 and the push-pull frame 33 are driven to rotate by the rotary drive 31, and the push-pull frame 33 is moved to the inlet and outlet 18 of the shell 10.

[0054] Next, the pipette 20 is placed on the push-pull frame 33 via the cross plate 21 and connected to the first positioning pin 332 through the first positioning hole 211. Then, the support 32 is rotated by the rotary drive 31, and the push-pull frame 33 is rotated to face the door panel 11 of a certain disinfection chamber 12. Then, the push-pull frame 33 is extended relative to the support 32 by the telescopic drive 34. During the extension and movement of the push-pull bracket 33, the front end of the boss 331 pushes the side of the swing bracket 53, causing the swing bracket 53 to rotate around the pivot 531. When the swing bracket 53 rotates, it pushes the pin 523 to make the rack 52 overcome the elastic force of the first spring 51 and slide along the guide sleeve 14. When the rack 52 slides, it drives the gear 112, the hinge shaft 111 and the door panel 11 to rotate, opening the door panel 11. When the swing bracket 53 rotates to the point where its end contacts the upper surface of the boss 331, it stops rotating. The door panel 11 maintains a fixed opening. Then the push-pull bracket 33 continues to extend and move, sending the pipette 20 into the disinfection chamber 12.

[0055] When the pipette 20 moves to the position where the second positioning hole 212 is above and coaxially aligned with the second positioning pin 411, the push-pull bracket 33 stops moving. At this time, the upper end of the column 17 abuts against the bottom surface of the pressure plate 42, and the second spring 43 is in a compressed state. Then, the lifting drive 70 drives the lifting frame 80, the rotation drive 60, and the bracket 41 to move upward, so that the second positioning pin 411 is inserted into the second positioning hole 212. Then, the bracket 41 lifts the horizontal plate 21 and disengages it from the push-pull bracket 33 and the first positioning pin 332. Next, the bracket 41 continues to move upward until the pressure plate 42 separates from the column 17. At this time, the pressure plate 42 is pressed against the upper surface of the horizontal plate 21 under the elastic force of the second spring 43, thus pressing and fixing the pipette 20.

[0056] When the bracket 41 moves up to a height higher than the column 17 and stops moving, the telescopic drive 34 drives the push-pull bracket 33 to retract relative to the support 32, causing the push-pull bracket 33 to exit from the disinfection chamber 12 until the swing bracket 53 separates from the boss 331. At this time, the rack 52 slides back to its original position under the elastic force of the first spring 51, and drives the door panel 11 to close. Then, the rotation drive 60 drives the bracket 41 and the pipette 20 to rotate, and at the same time, the ultraviolet lamp 90 is turned on to disinfect the pipette 20 with ultraviolet light. Since the pipette 20 is rotating during the disinfection process, the exterior of the pipette 20 can be thoroughly disinfected, thereby improving the disinfection effect.

[0057] When it is necessary to remove the pipette 20 from the disinfection chamber 12, the working process is the reverse of the steps described above for inserting the pipette 20 into the disinfection chamber 12, and will not be described in detail here.

[0058] The embodiments described in this specific implementation are preferred embodiments of this application and are not intended to limit the scope of protection of this application. Identical components are represented by the same reference numerals. Therefore, all equivalent changes made to the structure, shape, and principle of this application should be covered within the scope of protection of this application.

Claims

1. A pipette for cell culture, characterized in that, include: Pipettes (20); The housing (10) has multiple disinfection chambers (12) inside. The transfer mechanism (30) is used to transfer the pipette (20) between the inside and outside of the disinfection chamber (12). The side wall of the housing (10) and the disinfection chamber (12) are respectively provided with door panels (11) that can be opened during transfer and closed after transfer. Positioning element (40), the positioning element (40) is respectively disposed in the disinfection chamber (12) and is used to position the pipette (20) transferred by the transfer mechanism (30) into the disinfection chamber (12); The disinfection component is disposed within the disinfection chamber (12).

2. A pipette for cell culture according to claim 1, characterized in that, The transfer mechanism (30) includes a rotary drive (31), a support (32), and a push-pull frame (33). The rotary drive (31) is fixed on the housing (10). The support (32) is connected to the rotary drive (31). The push-pull frame (33) is telescopically mounted on the support (32). The support (32) is provided with a telescopic drive (34) for driving the push-pull frame (33) to extend and retract relative to the support (32). The push-pull frame (33) is used to support the pipette (20). The push-pull frame (33) is provided with a first limiting part for limiting the position of the pipette (20).

3. A pipette for cell culture according to claim 2, characterized in that, It also includes an opening and closing mechanism (50), which is disposed on the housing (10). The push-pull bracket (33) can act on the opening and closing mechanism (50) and drive the door panel (11) to open through the opening and closing mechanism (50). When the push-pull bracket (33) is separated from the opening and closing mechanism (50), the opening and closing mechanism (50) can drive the door panel (11) to close.

4. A pipette for cell culture according to claim 3, characterized in that, The opening and closing mechanism (50) includes a first elastic element and a transmission assembly. A hinge shaft (111) is fixed on the door panel (11). The door panel (11) is hinged to the housing (10) through the hinge shaft (111). The transmission assembly is located on the housing (10) and connected to the hinge shaft (111). The push-pull bracket (33) can act on the transmission assembly and drive the hinge shaft (111) to rotate through the transmission assembly. When the push-pull bracket (33) is separated from the opening and closing mechanism (50), the first elastic element can act on the hinge shaft (111) and drive the hinge shaft (111) to reverse.

5. A pipette for cell culture according to claim 1, characterized in that, It also includes a rotary drive (60), the disinfection component is an ultraviolet lamp (90), the rotary drive (60) is disposed in the disinfection chamber (12), the rotary drive (60) is connected to the positioning component (40) and is used to drive the positioning component (40) to rotate.

6. A pipette for cell culture according to claim 5, characterized in that, It also includes a lifting drive (70) and a lifting frame (80). The lifting drive (70) is fixed on the housing (10). The lifting frame (80) is connected to the lifting drive (70). The rotating drive (60) is fixed on the lifting frame (80). The positioning member (40) includes a bracket (41). The bracket (41) is connected to the rotating drive (60) and is used to support the pipette (20). The bracket (41) is provided with a second limiting part for limiting the position of the pipette (20).

7. A pipette for cell culture according to claim 6, characterized in that, The positioning member (40) further includes a pressure plate (42) and a second elastic member. The pressure plate (42) is slidably disposed on the bracket (41) in a vertical direction. The second elastic member acts on the pressure plate (42) and can push the pressure plate (42) to press against the pipette (20) from top to bottom, so as to restrict the vertical movement of the pipette (20).

8. A pipette for cell culture according to claim 7, characterized in that, The bottom wall of the disinfection chamber (12) of the housing (10) is provided with a column (17), the upper end of the column (17) can abut against the bottom surface of the pressure plate (42) to limit the travel of the pressure plate (42) as it moves downward with the lifting frame (80).

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