An online chromatography device

Abstract

This utility model discloses an online chromatography device, belonging to the field of bioengineering technology. The device includes a system pump, a bubble trap, a filter, and a chromatography column. The bottom of the bubble trap is connected to the outlet of the system pump via pipe A, and the top of the bubble trap is equipped with an vent valve A. The bottom of the filter is connected to the bottom of the bubble trap via pipe D, and pipe D is equipped with a valve D. The top of the chromatography column is connected to the bottom of the filter via pipe B. When gas is detected entering the bubble trap and the liquid level inside the bubble trap reaches the low level limit, the vent valve A at the top of the bubble trap is opened to release the gas, while valve D is closed. This ensures that the liquid inside the bubble trap is not emptied, further preventing gas from entering the chromatography column, ensuring the performance of the chromatography column, and thus avoiding the need for repacking the chromatography column.

Description

Technical Field

[0001] This utility model relates to an online chromatography device, belonging to the field of bioengineering technology. Background Technology

[0002] The existing online chromatography equipment in the production workshop, such as Figure 1 As shown, during the product chromatography process, the chromatography feed solution flows sequentially through the bubble trap 2, filter 4 and chromatography column 5 under the action of system pump 1, and then the chromatographic feed solution is collected.

[0003] When gas enters bubble trap 2, the vent valve A20 at the top of bubble trap 2 needs to be opened to vent the gas when the liquid level in bubble trap 2 reaches the low liquid level limit 3. However, during the venting process of vent valve A20, if the venting rate of bubble trap 2 is too low, resulting in untimely venting, or if the venting rate of bubble trap 2 is too high, bubble trap 2 may be emptied, leading to gas ingress into chromatography column 5. Gas ingress into chromatography column 5 will affect its chromatography performance, requiring repacking of chromatography column 5. Utility Model Content

[0004] To solve the above-mentioned technical problems, this utility model provides an online chromatography device.

[0005] This utility model is achieved through the following technical solution:

[0006] An online chromatography device includes a system pump, a bubble trap, a filter, and a chromatography column. The bottom of the bubble trap is connected to the outlet of the system pump via a pipe A, and the top of the bubble trap is provided with an exhaust valve A. The bottom of the filter is connected to the bottom of the bubble trap via a pipe D, and a valve D is provided on the pipe D. The top of the chromatography column is connected to the bottom of the filter via a pipe B.

[0007] The filter is equipped with an exhaust valve B at its top.

[0008] The bottom of the chromatography column is equipped with a drain pipe.

[0009] The middle part of the drain pipe is connected to the middle part of the pipe B via pipe C, and a valve C is installed on pipe C. A valve A is installed on pipe B between pipe C and the chromatography column, and a valve B is installed on the drain pipe between pipe C and the chromatography column.

[0010] Valve A is located near the connection point between pipe C and pipe B.

[0011] Valve B is located near the connection point between pipe C and drain pipe.

[0012] Valves A, B, C, and D are all ball valves.

[0013] The beneficial effects of this utility model are as follows: when gas is detected entering the bubble trap and the liquid level in the bubble trap reaches the low liquid level limit, the vent valve A at the top of the bubble trap is opened to vent the gas, while valve D is closed at the same time. This ensures that the liquid in the bubble trap will not be emptied, further preventing gas from entering the chromatography column, ensuring the performance of the chromatography column, and thus avoiding the need to repack the chromatography column. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the existing online chromatography device in the production workshop.

[0015] Figure 2 This is a schematic diagram of the structure of this utility model.

[0016] In the diagram: 1-System pump, 2-Bubble trap, 20-Exhaust valve A, 3-Low level limit, 4-Filter, 40-Exhaust valve B, 5-Chromatography column, 6-Pipeline A, 7-Valve A, 8-Pipeline B, 9-Drain pipe, 10-Valve B, 11-Valve C, 12-Pipeline C, 13-Pipeline D, 14-Valve D. Detailed Implementation

[0017] The technical solution of this utility model is further described below, but the scope of protection is not limited to what is described.

[0018] like Figure 2 As shown, the present invention describes a method such as... Figure 1 As shown, an online chromatography apparatus includes a system pump 1, a bubble trap 2, a filter 4, and a chromatography column 5. The bottom of the bubble trap 2 is connected to the outlet of the system pump 1 via pipe A6, and the top of the bubble trap 2 is equipped with an exhaust valve A20. The bottom of the filter 4 is connected to the bottom of the bubble trap 2 via pipe D13, and a valve D14 is installed on pipe D13. The top of the chromatography column 5 is connected to the bottom of the filter 4 via pipe B8. In use, the chromatography feed solution flows sequentially through pipe A6, bubble trap 2, pipe D13, filter 4, and pipe B8 under the action of the system pump 1 before entering the chromatography column 5. When gas is detected entering the bubble trap 2 and the liquid level in the bubble trap 2 reaches the low liquid level limit 3, the exhaust valve A20 at the top of the bubble trap 2 is opened to release the gas, while valve D14 is closed. This ensures that the feed solution in the bubble trap 2 is not emptied, further preventing air from entering the chromatography column 5, ensuring the performance of the chromatography column 5, and thus avoiding the need for repacking the chromatography column 5. Once the gas in bubble trap 2 is expelled, close exhaust valve A20 and open valve D14 to continue chromatography of the liquid.

[0019] An exhaust valve B40 is installed on the top of the filter 4. This allows the filter 4 to release air through the exhaust valve B40.

[0020] The bottom of the chromatography column 5 is provided with a drain pipe 9.

[0021] The middle section of the drain pipe 9 is connected to the middle section of the pipe B8 via pipe C12, and a valve C11 is installed on pipe C12. A valve A7 is installed on pipe B8 between pipe C12 and the chromatography column 5, and a valve B10 is installed on the drain pipe 9 between pipe C12 and the chromatography column 5. If air bubbles are generated in the pipeline during the replacement of filter 4, bypass venting can be performed by closing valves A7 and B10 and opening valve C11 to prevent air bubbles in the pipeline from entering the chromatography column 5 along pipe B8.

[0022] Valve A7 is located near the connection point between pipe C12 and pipe B8. This prevents air bubbles in the pipeline from entering pipe B8 between pipe C12 and the chromatography column 5 during bypass venting.

[0023] The valve B10 is located near the connection point between the pipe C12 and the drain pipe 9.

[0024] Valve A7, valve B10, valve C11 and valve D14 are all ball valves.

Claims

1. An online chromatography apparatus, characterized in that: The system includes a system pump (1), a bubble trap (2), a filter (4), and a chromatography column (5). The bottom of the bubble trap (2) is connected to the outlet of the system pump (1) through a pipe A (6), and the top of the bubble trap (2) is equipped with an exhaust valve A (20). The bottom of the filter (4) is connected to the bottom of the bubble trap (2) through a pipe D (13), and a valve D (14) is provided on the pipe D (13). The top of the chromatography column (5) is connected to the bottom of the filter (4) through a pipe B (8).

2. The online chromatography apparatus as described in claim 1, characterized in that: The filter (4) is provided with an exhaust valve B (40) at the top.

3. The online chromatography apparatus as described in claim 1, characterized in that: The bottom of the chromatography column (5) is provided with a drain pipe (9).

4. The online chromatography apparatus as described in claim 3, characterized in that: The middle part of the drain pipe (9) is connected to the middle part of the pipe B (8) through the pipe C (12), and a valve C (11) is installed on the pipe C (12), a valve A (7) is installed on the pipe B (8) between the pipe C (12) and the chromatography column (5), and a valve B (10) is installed on the drain pipe (9) between the pipe C (12) and the chromatography column (5).

5. The online chromatography apparatus as described in claim 4, characterized in that: The valve A (7) is located near the connection point between pipe C (12) and pipe B (8).

6. The online chromatography apparatus as described in claim 4, characterized in that: The valve B (10) is located near the connection point between the pipe C (12) and the drain pipe (9).

7. The online chromatography apparatus as described in claim 4, characterized in that: Valve A (7), valve B (10), valve C (11) and valve D (14) are all ball valves.

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