A culture device for mesenchymal stem cell therapy of osteoarthritis

Abstract

This utility model discloses a culture device for mesenchymal stem cell therapy of osteoarthritis, belonging to the field of culture devices. It includes a culture vessel with an opening at the top; a viewing window embedded in the top of the culture vessel; a rotating mechanism and a support member, respectively disposed on the bottom wall of the inner cavity of the culture vessel; several support members, respectively disposed on the outer wall of the rotating mechanism; culture dishes are placed on the support members; the rotating mechanism drives the support members and culture dishes to rotate; the support members lift the culture dishes on the corresponding support members to below the viewing window; and a driving member, penetrating the side wall of the culture vessel and connected to the support members, provides power to the support members. This utility model's culture device for mesenchymal stem cell therapy of osteoarthritis reduces the chance of direct contact between the culture dishes and the outside environment without affecting the observation of the culture dishes, thus better protecting the stem cell culture environment.

Description

Technical Field

[0001] This utility model belongs to the field of culture devices, specifically relating to a culture device for mesenchymal stem cell therapy of osteoarthritis. Background Technology

[0002] Mesenchymal stem cells (MSCs) are important members of the stem cell family, originating from the mesoderm in early development. They are pluripotent stem cells, initially discovered in bone marrow, and have gained increasing attention due to their multipotent differentiation potential, hematopoietic support, promotion of stem cell engraftment, immune regulation, and self-replication. In the process of culturing mesenchymal stem cells, it is necessary to ensure that the culture medium is kept in an atmosphere with 5% CO2.

[0003] When culturing stem cells, it is necessary to continuously observe and monitor their culture status. However, the aforementioned open handling method allows the stem cell culture medium to come into direct contact with the outside air, which may affect the air environment required for the culture medium and the CO2 content needed for stem cell growth, leading to culture failure or even infection. To solve the above problems, a culture device for mesenchymal stem cell therapy for osteoarthritis is proposed. Utility Model Content

[0004] In view of one or more of the above-mentioned defects or improvement needs of the prior art, the present invention provides a culture device for mesenchymal stem cell therapy for osteoarthritis, which has the advantage of being easy to observe.

[0005] To achieve the above objectives, this utility model provides a culture device for mesenchymal stem cell therapy of osteoarthritis, including a culture vessel with an opening at the top;

[0006] A viewing window is embedded in the top of the incubator;

[0007] The rotating mechanism and the supporting component are respectively installed on the bottom wall of the inner cavity of the culture vessel;

[0008] Several load-bearing components are respectively installed on the outer wall of the rotating mechanism;

[0009] The carrier is used to place the culture dish, the rotating mechanism is used to drive the carrier and the culture dish to rotate, and the support is used to lift the culture dish on the corresponding carrier to the bottom of the viewing window.

[0010] A drive component, which runs through the side wall of the incubator and is connected to the support component, is used to provide power to the support component;

[0011] A cover plate, hinged to the top of the incubator, is used to close the opening at the top of the incubator.

[0012] Furthermore, the rotating mechanism includes a support frame disposed on the bottom wall of the inner cavity of the culture vessel; a turntable rotatably disposed on the support frame; and a servo motor disposed on the bottom wall of the inner cavity of the culture vessel and located in the support frame, with the output end of the servo motor connected to the turntable.

[0013] Furthermore, the support includes two rotating shafts; a support plate rotatably sleeved on the outer wall of the rotating shafts, with the lower support plate set on the bottom wall of the inner cavity of the incubator and a lifting frame set at the top of the upper support plate; two drive wheels rotatably set at one end of the two rotating shafts respectively; a connecting plate rotatably set at the other end of the two rotating shafts; and the two drive wheels are connected by a drive belt.

[0014] Furthermore, the carrier includes two support arms respectively disposed on the outer wall of the turntable; and a support tray disposed at the top of the support arms.

[0015] Furthermore, the driving component includes a rotating rod that can be rotatably passed through the side wall of the incubator via a rotating sealing plug. One end of the rotating rod is connected to the lower rotating shaft, and the other end is provided with a handle.

[0016] Furthermore, a fixing plate is set on each side of the incubator, and two limiting frames are set on the top of the fixing plate. The cross surface of the limiting frame is L-shaped, and a sliding plate is slidably set inside the two limiting frames on one side. Several magnifying glasses are embedded on the top of the sliding plate.

[0017] In summary, the beneficial effects of the above-described technical solutions conceived by this utility model compared with the prior art include:

[0018] The mesenchymal stem cell culture device of this invention for treating osteoarthritis can reduce the chance of direct contact between the culture dish and the outside world without affecting the observation of the culture dish, thus better protecting the culture environment of stem cells. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the structure of this utility model;

[0020] Figure 2 This is a schematic diagram of the cross-sectional structure of the culture device of this utility model;

[0021] Figure 3 This is a bottom view of the rotating mechanism of this utility model;

[0022] Figure 4 This is a schematic diagram of the support structure of this utility model.

[0023] In all the accompanying drawings, the same reference numerals denote the same technical features, specifically: 1. Incubator; 2. Viewing window; 3. Rotating mechanism; 31. Support frame; 32. Turntable; 33. Servo motor; 4. Support component; 41. Rotating shaft; 42. Support plate; 43. Lifting frame; 44. Transmission wheel; 45. Connecting plate; 46. Transmission belt; 5. Support component; 51. Support arm; 52. Support tray; 6. Driving component; 7. Fixing plate; 8. Limiting frame; 9. Slide plate; 10. Magnifying glass; 11. Cover plate. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0025] Please see Figure 1-4 The mesenchymal stem cell culture device for treating osteoarthritis in this embodiment has the advantage of being easy to observe.

[0026] Specifically, it includes a culture vessel 1 with an opening at the top;

[0027] Viewing window 2 is embedded in the top of the incubator 1, and the viewing window 2 is made of transparent glass.

[0028] The rotating mechanism 3 and the supporting component 4 are respectively installed on the bottom wall of the inner cavity of the incubator 1;

[0029] Several support components 5 are respectively installed on the outer wall of the rotating mechanism 3;

[0030] The carrier 5 is used to place the culture dish, the rotating mechanism 3 is used to drive the carrier 5 and the culture dish to rotate, and the support 4 is used to lift the culture dish on the corresponding carrier 5 to below the viewing window 2.

[0031] The driving component 6 is disposed through the side wall of the incubator 1 and connected to the support component 4, and is used to provide power to the support component 4;

[0032] The cover plate 11 is hinged to the top of the culture vessel 1 and is used to close the opening at the top of the culture vessel 1.

[0033] In this embodiment, before use, multiple culture dishes need to be placed on different support members 5, and then the cover plate 11 is closed to form a closed state inside the culture vessel 1 and culture is carried out through the culture vessel 1. When it is necessary to observe the culture dish, the rotating mechanism 3 rotates to transport the corresponding culture dish to the position opposite to the support member 4. Then, by rotating the drive member 6, the support member 4 can move the culture dish on the corresponding support member 5 to the bottom of the viewing window plate 2 for observation. In this way, the probability of the culture dish directly contacting the outside world can be reduced without affecting the observation of the culture dish, so that the culture environment of stem cells can be better protected.

[0034] Specifically, refer to Figure 3 The rotating mechanism 3 includes a support frame 31 disposed on the bottom wall of the inner cavity of the culture vessel 1; a turntable 32 rotatably disposed on the support frame 31; and a servo motor 33 disposed on the bottom wall of the inner cavity of the culture vessel 1 and located in the support frame 31, with the output end of the servo motor 33 connected to the turntable 32.

[0035] In this embodiment, when the servo motor 33 is powered on, it can drive the turntable 32 to rotate so as to rotate the corresponding carrier 5 to a position opposite to the support 4.

[0036] Specifically, refer to Figure 4 The support member 4 includes two rotating shafts 41; a support plate 42 rotatably sleeved on the outer wall of the rotating shafts 41, with the lower support plate 42 set on the bottom wall of the inner cavity of the incubator 1 and a lifting frame 43 set at the top of the upper support plate 42; two drive wheels 44 rotatably set at one end of the two rotating shafts 41 respectively; a connecting plate 45 rotatably set at the other end of the two rotating shafts 41; and the two drive wheels 44 are connected by a drive belt 46.

[0037] Specifically, refer to Figure 3 The support member 5 includes two support arms 51 respectively disposed on the outer wall of the turntable 32; and a support tray 52 disposed at the top of the support arms 51, the two support trays 52 being able to support the bottom sides of the culture dish.

[0038] In this embodiment, when the lower rotating shaft 41 rotates, the upper rotating shaft 41 and the support plate 42 can rotate with the lower rotating shaft 41 as the center through the drive of the connecting plate 45. At the same time, through the cooperation of the transmission wheel 44 and the transmission belt 46, the upper rotating shaft 41 can be driven to rotate and the angle of the support plate 42 can be adjusted so that the lifting frame 43 is always in a horizontal state. When the lifting frame 43 passes between the two support trays 52, it can support and connect the culture dish. As the support plate 42 continues to rotate, the culture dish can be moved away from the inside of the two support trays 52 and moved to the bottom of the viewing window plate 2 for observation.

[0039] Specifically, refer to Figure 1The driving component 6 includes a rotating rod 62 that can be rotatably passed through the side wall of the incubator 1 via a rotating sealing plug 61. One end of the rotating rod 62 is connected to the lower rotating shaft 41, and the other end is provided with a handle 63.

[0040] In this embodiment, when the handle 63 is rotated, the rotating rod 62 can drive the lower rotating shaft 41. The rotating sealing plug 61 can rotate and seal the incubator 1 and the rotating rod 62 to prevent air from leaking out from the gap between them.

[0041] Specifically, refer to Figure 1 The incubator 1 has a fixing plate 7 on each side. The top of the fixing plate 7 has two limiting frames 8. The horizontal surface of the limiting frame 8 is L-shaped. The two limiting frames 8 on one side have a sliding plate 9 inside. The top of the sliding plate 9 is embedded with several magnifying glasses 10. Each magnifying glass 10 has a different magnification.

[0042] In this embodiment, when penetrating the culture dish, the slide plate 9 can be pulled to slide so that the corresponding magnifying glass 10 can be adjusted to the position opposite to the viewing window plate 2. Through the magnification of the magnifying glass 10, the culture dish can be observed better. The sliding design of the slide plate 9 allows the use of a magnifying glass 10 with the corresponding magnification according to the usage requirements.

[0043] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A culture device for mesenchymal stem cell therapy for osteoarthritis, characterized by, Includes a culture vessel (1), with an opening at the top of the culture vessel (1); A viewing window (2) is embedded in the top of the incubator (1); The rotating mechanism (3) and the support (4) are respectively installed on the bottom wall of the inner cavity of the incubator (1); Several support components (5) are respectively installed on the outer wall of the rotating mechanism (3); The carrier (5) is used to place the culture dish, the rotating mechanism (3) is used to drive the carrier (5) and the culture dish to rotate, and the support (4) is used to lift the culture dish on the corresponding carrier (5) to below the viewing window (2). A drive unit (6) is disposed through the side wall of the incubator (1) and connected to the support unit (4) to provide power to the support unit (4); A cover plate (11) is hinged to the top of the incubator (1) and is used to close the opening at the top of the incubator (1).

2. The device for culturing mesenchymal stem cells for treating osteoarthritis according to claim 1, wherein The rotating mechanism (3) includes a support frame (31) disposed on the bottom wall of the inner cavity of the culture vessel (1); a turntable (32) rotatably disposed on the support frame (31); and a servo motor (33) disposed on the bottom wall of the inner cavity of the culture vessel (1) and located in the support frame (31), with the output end of the servo motor (33) connected to the turntable (32).

3. The mesenchymal stem cell culture device for treating osteoarthritis according to claim 1, characterized in that, The support member (4) includes two rotating shafts (41); a support plate (42) rotatably sleeved on the outer wall of the rotating shaft (41), the lower support plate (42) is set on the bottom wall of the inner cavity of the incubator (1), and a lifting frame (43) is set on the top of the upper support plate (42); two drive wheels (44) rotatably set at one end of the two rotating shafts (41); a connecting plate (45) rotatably set at the other end of the two rotating shafts (41); and the two drive wheels (44) are connected by a drive belt (46).

4. The mesenchymal stem cell culture device for treating osteoarthritis according to claim 2, characterized in that, The carrier (5) includes two support arms (51) respectively disposed on the outer wall of the turntable (32); and a support tray (52) disposed at the top of the support arms (51).

5. The mesenchymal stem cell culture device for treating osteoarthritis according to claim 3, characterized in that, The drive unit (6) includes a rotating rod (62) that can be rotatably passed through the side wall of the incubator (1) by rotating the sealing plug (61). One end of the rotating rod (62) is connected to the lower rotating shaft (41), and the other end is provided with a handle (63).

6. The culture device for mesenchymal stem cell therapy for osteoarthritis according to claim 1, characterized in that, The incubator (1) has a fixing plate (7) on each side. The top of the fixing plate (7) has two limiting frames (8). The cross surface of the limiting frame (8) is L-shaped. The two limiting frames (8) on one side have a sliding plate (9) inside. The top of the sliding plate (9) has several magnifying glasses (10).

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