Multidirectional shooting sample holder assembly for light sheet microscope

Abstract

The utility model provides a multidirectional shooting sample holder assembly used for a light sheet microscope. The multidirectional shooting sample holder assembly comprises a support used for being installed on the light sheet microscope; the base is connected with the bracket and is provided with a plurality of limiting feet; the sample frame can be detachably connected with the base so as to allow the sample frame to be mounted in the mounting area defined by the limiting feet again after being mounted in a 180-degree reversing manner; the light-transmitting clamping pieces are arranged on the sample frame, the light-transmitting clamping pieces are arranged in a paired mode, and a sample clamping area is arranged between the light-transmitting clamping pieces arranged in the paired mode. In conclusion, by the adoption of the sample frame assembly, the shooting angle of the sample frame can be adjusted by changing the installation posture of the sample frame, shooting imaging and 3D reconstruction of the sample at multiple angles are achieved, and the obtained sample image information has better definition and comprehensiveness.

Description

Technical Field

[0001] This utility model relates to the field of optical imaging technology, specifically to a multi-directional imaging sample holder assembly for a light sheet microscope. Background Technology

[0002] Light sheet microscopy is a microscopic imaging system designed specifically for transparent tissues, organoids, and large-volume samples. However, existing non-suspended light sheet microscopy systems typically only support unidirectional imaging. Due to the limitations of the objective lens's imaging distance and the effects of light scattering and absorption, the detection of deep signals in the sample is greatly limited, as is the comprehensiveness and depth of the imaging. This makes such light sheet microscopy unsuitable for imaging opaque organoid samples and 3D samples with only surface transparency, and it is difficult to provide clear and complete three-dimensional structural information of the sample. Utility Model Content

[0003] This invention is made to solve the above-mentioned technical problems. Its purpose is to provide a multi-directional imaging sample holder assembly for a light sheet microscope, which can improve the clarity and comprehensiveness of the image by shooting from different directions.

[0004] This utility model discloses a multi-directional imaging sample holder assembly for a light sheet microscope, comprising: a support for mounting to the light sheet microscope; a base connecting the support, the base being provided with a plurality of limiting feet; a sample holder, the sample holder being detachably connected to the base to allow the sample holder to be repositioned 180° and then reinstalled in the mounting area enclosed by the plurality of limiting feet; and light-transmitting clips provided on the sample holder, the light-transmitting clips being paired and arranged, and the space between the paired light-transmitting clips being a sample holding area.

[0005] Optionally, the sample holder may have two different shooting angles.

[0006] Optionally, one of the base and the sample holder is provided with a through hole, and the other is provided with a rotating shaft that can be rotatably inserted into the through hole.

[0007] Optionally, at least one of the base and the sample holder may have a hollow structure.

[0008] Optionally, multiple hollow structures are arranged around the through holes, and / or multiple hollow structures are arranged around the pivot.

[0009] Optionally, the sample holder is equipped with slots, the slots are paired, and the light-transmitting clips are inserted into the slots one by one.

[0010] Optionally, the light-transmitting clip is bonded to the slot.

[0011] Optionally, the bracket includes a first connecting plate and a second connecting plate that are connected to each other, the first connecting plate and the second connecting plate having different extending directions; the first connecting plate is connected to the base.

[0012] Optionally, the second connecting plate is provided with positioning magnets.

[0013] Optionally, the support includes a support body and a boss, and the base includes a base body and an extension extending out of the base body, the extension fitting the end face of the support body; the base and the boss are arranged opposite to each other along the length direction of the sample holder assembly.

[0014] The beneficial effects of this utility model are as follows:

[0015] This utility model discloses a multi-directional imaging sample holder assembly for a light sheet microscope, comprising: a support for mounting to the light sheet microscope; a base connecting the support, the base being provided with a plurality of limiting feet; a sample holder, the sample holder being detachably connected to the base to allow the sample holder to be repositioned 180° and then reinstalled in the mounting area enclosed by the plurality of limiting feet; and light-transmitting clips provided on the sample holder, the light-transmitting clips being paired and arranged, and the space between the paired light-transmitting clips being a sample holding area.

[0016] In summary, by using the sample holder assembly of this application, the shooting angle of the sample holder can be adjusted by changing the installation posture of the sample holder, thereby enabling imaging and 3D reconstruction of the sample from multiple angles. This reduces the influence of factors such as the imaging distance limitation of the objective lens, light scattering, and light absorption on imaging, as well as the constraints on the detection of deep signals in the sample, thus improving the comprehensiveness and depth of imaging. It is suitable for imaging various types of samples, including opaque organoid samples and 3D samples with only surface transparency, and the final sample image information has better clarity and comprehensiveness. Attached Figure Description

[0017] The above-described features and advantages of this invention can be better understood after reading the following detailed description of the embodiments of this disclosure in conjunction with the accompanying drawings. In the drawings, the components are not necessarily drawn to scale, and components having similar related characteristics or features may have the same or similar reference numerals.

[0018] Figure 1 This is an exploded view of a sample holder imaging assembly according to an embodiment of this utility model;

[0019] Figure 2 This is a sample holder of the utility model. Figure 1 Assembly diagram;

[0020] Figure 3 This is a utility model Figure 1 Structural diagram of the central base;

[0021] Figure 4 This is a utility model Figure 1 A structural diagram of the sample holder from one perspective;

[0022] Figure 5 This is a utility model Figure 1 Another structural view of the sample holder;

[0023] Figure 6 This is a utility model Figure 1 Structural diagram of the mid-support;

[0024] Figure 7 This is an exploded view of the sample holder imaging component according to another embodiment of this utility model;

[0025] Figure 8 yes Figure 7 Assembly diagram;

[0026] Figure 9 This is a utility model Figure 7 A diagram of the support structure;

[0027] Figure 10 This is a utility model Figure 7 The base structure diagram.

[0028] Explanation of reference numerals in the attached figures:

[0029] 10-Hollow structure,

[0030] 100-Standard

[0031] 110 - First connecting plate, 120 - Second connecting plate, 130 - Positioning magnet, 140 - Bracket body, 150 - Boss

[0032] 200-base,

[0033] 210 - Limiting foot, 220 - Through hole, 201 - Base body, 202 - Extension, 2021 - Beveled surface

[0034] 300-sample rack

[0035] 310 - Spindle, 320 - Slot

[0036] 400-Transparent clip. Detailed Implementation

[0037] The present invention will be further described below with reference to specific embodiments and accompanying drawings. More details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention can obviously be implemented in many other ways different from those described herein. Those skilled in the art can make similar extensions and derivations based on actual application situations without departing from the spirit of the present invention. Therefore, the scope of protection of the present invention should not be limited by the content of this specific embodiment.

[0038] It should be noted that these and other accompanying drawings are merely examples and are not drawn to scale, and should not be construed as limiting the scope of protection of this utility model.

[0039] The following is combined Figures 1-10 This utility model is hereby introduced.

[0040] Example 1

[0041] Figure 1 This is an exploded view of a sample holder imaging assembly according to an embodiment of this utility model; Figure 2 This is a sample holder of this utility model under a certain shooting angle. Figure 1 Assembly diagram. For example... Figures 1-2 As shown, this application discloses a multi-directional imaging sample holder assembly for a light sheet microscope, including: a support 100, a base 200, a sample holder 300, and a light-transmitting clip 400.

[0042] The support 100 serves as the mounting base for this application and can be designed according to different models of light sheet microscopes, allowing it to be mounted to the imaging platform of the light sheet microscope via magnetic attraction, plug-in connection, or other methods. The base 200 connects to the support 100 and is equipped with several limiting feet 210. The area enclosed by these limiting feet 210 forms the mounting area for the sample holder 300. The base 200 can be made of a material resistant to organic solvents, such as photosensitive resin, to ensure that the sample holder assembly of this application is not corroded by organic solvents during use. The sample holder 300 can be detachably connected to the base 200 and mounted in the mounting area, and its shooting angle can be adjusted within the mounting area to achieve multi-angle imaging of the sample. A light-transmitting clip 400 is disposed on the sample holder 300. The light-transmitting clips 400 are paired and arranged, and the area between the paired light-transmitting clips 400 is a sample holding area. In use, the sample is placed between the paired light-transmitting clips 400. The holding force of the light-transmitting clips 400 ensures the stability of the sample during the imaging process. The light-transmitting clips 400 can be made of transparent materials such as glass in the form of cover glass to improve their light transmittance, thereby facilitating the imaging of the sample. At the same time, the spacing between the light-transmitting clips 400 is adjustable to achieve the clamping of samples of different sizes and shapes, thereby improving the applicability of the device of this application.

[0043] The device described in this application is used as follows:

[0044] In use, first place the sample holder assembly of this application in the imaging area of ​​the light sheet microscope, and then clamp the bracket 100 to fix the relative position of the sample holder assembly.

[0045] Then install the sample holder 300, which is adapted to the sample size and shape, and position the sample holder 300 and the sample clamped on it at one of the shooting angles, for example... Figure 2The shooting angle shown.

[0046] The sample is imaged at this shooting angle using a light-sheet microscope, thus obtaining image information of the sample at this shooting angle.

[0047] The sample holder 300 is rotated 180° to place it at another shooting angle, and then reinstalled into the mounting area enclosed by several limiting feet 210.

[0048] The sample was photographed from one shooting angle using a light sheet microscope, thus obtaining image information of the sample from another shooting angle.

[0049] Thus, by using the sample holder assembly of this application for imaging, image information of the sample can be obtained from multiple shooting angles, and then the image information from multiple shooting angles can be stitched together to form complete image information.

[0050] The following analysis will further incorporate various sample cases:

[0051] Case 1: In the observation of biological samples, by changing the installation posture of the sample holder 300, imaging of opaque organoids stained with propidium iodide (PI) can be achieved at different shooting angles. Compared with the imaging results in related technologies, the imaging results of this application have higher clarity and better completeness.

[0052] In Case 2, the green cells in the sample are chondrocytes, and the red cells are fibroblasts. In the observation of biological samples, by changing the installation posture of the sample holder 300, imaging of the 3D printed hollow transparent biological sample from different shooting angles can be achieved. Compared with the imaging results in related technologies, the imaging results of this application have higher clarity and better completeness.

[0053] Case 3: In the observation of biological samples, by changing the installation posture of the sample holder 300, imaging of the transparent mouse heart can be achieved from different shooting angles. Compared with the imaging results in related technologies, the imaging results of this application have higher clarity and better completeness.

[0054] Case 4: In the observation of biological samples, by changing the installation posture of the sample holder 300, imaging of DAPI-stained opaque organoids at different shooting angles can be achieved. Compared with the imaging results in related technologies, the imaging results of this application have higher clarity and better completeness.

[0055] Case 5: In the observation of biological samples, by changing the installation posture of the sample holder 300, imaging can be achieved at different shooting angles of the hemispherical retina. Compared with the imaging results in related technologies, the imaging results of this application have higher clarity and better completeness.

[0056] Case 6: In the observation of biological samples, by changing the installation posture of the sample holder 300, imaging of mouse embryos can be achieved from different shooting angles. Compared with the imaging results in related technologies, the imaging results of this application have higher clarity and better completeness.

[0057] In summary, by using the sample holder assembly of this application, the shooting angle of the sample holder 300 can be adjusted by adjusting the installation posture of the sample holder 300, thereby enabling the imaging and 3D reconstruction of the sample from multiple angles. This reduces the influence of factors such as the imaging distance limitation of the objective lens, light scattering, and light absorption on the imaging, as well as the constraints on the detection of deep signals of the sample, improving the comprehensiveness and depth of the imaging. It is suitable for imaging various samples, including opaque organoid samples and 3D samples with only surface transparency, and the final sample image information has better clarity and comprehensiveness.

[0058] Optionally, Figure 3 This is a utility model Figure 1 The structural diagram of the base is shown in the figure. The base 200 is designed with mounting areas to allow the sample holder 300 to have different shooting angles, as detailed below:

[0059] Specifically, the base 200 is provided with four limiting feet 210, namely the first limiting foot, the second limiting foot, the third limiting foot and the fourth limiting foot; among them, the area between the first limiting foot and the second limiting foot, and the area between the third limiting foot and the fourth limiting foot are the installation areas.

[0060] like Figure 2 As shown, when the sample holder 300 is installed in the installation area, the first and second limiting feet stop the first side of the sample holder 300, and the third and fourth limiting feet stop the second side of the sample holder 300, thereby constraining the position of the sample holder 300 so that it is positioned at one of the shooting angles. After shooting at this angle, the sample holder 300 is removed and repositioned 180° to complete the imaging at another shooting angle. The first and second sides of the sample holder 300 are positioned opposite each other. In summary, through the stopping action of the limiting feet 210, the sample holder 300 can switch between two shooting angles with a 180° angle difference, and the error after switching is only 0.1 to 0.18 degrees, maintaining high accuracy.

[0061] Optionally, Figure 4 This is a utility model Figure 1 A structural diagram of the sample holder from one perspective; Figure 5 This is a utility model Figure 1 Another structural view of the sample holder; combined with Figures 3-5As shown, one of the base 200 and the sample holder 300 is provided with a through hole 220, and the other is provided with a rotating shaft 310 that is rotatably inserted into the through hole 220. For example, the base 200 is provided with a through hole 220 and the sample holder 300 is provided with a rotating shaft 310, or the base 200 is provided with a rotating shaft 310 and the sample holder 300 is provided with a through hole 220.

[0062] Thus, on the one hand, by rotating the sample holder 300 relative to the base 200, and with the limiting action of the limiting foot 210, the shooting angle of the sample holder 300 can be adjusted more accurately and conveniently. In the later 3D overall reconstruction of the image data, the imaging surface corresponding to the two sets of image data can be found easily and quickly, thereby improving the reconstruction accuracy and reconstruction efficiency. On the other hand, the stopping action of the limiting foot 210 can restrict the rotational freedom of the sample holder 300, and the cooperation between the through hole 220 and the rotating shaft 310 can restrict the movement freedom of the sample holder 300. In this way, the sample holder 300 can be fixed at the required shooting angle, which facilitates sample shooting and imaging.

[0063] Optionally, there can be a certain degree of interference fit or transition fit between the through hole 220 and the rotating shaft 310. This way, after the sample holder 300 is installed on the base 200, the sample holder 300 and the base 200 are not easily separated when no external force is applied, but are easy to separate when external force is applied. This allows for easy replacement of the sample holder 300 or adjustment of the shooting angle of the sample holder 300 without affecting its use.

[0064] Optionally, such as Figures 3-5 As shown, at least one of the base 200 and the sample holder 300 is provided with a hollow structure 10. The hollow structure 10 can reduce weight and save materials, and also reduce the contact surface between the base 200 and the sample holder 300, thereby reducing the resistance during the adjustment of the sample holder 300 and the resistance to the movement of the base 200 and the sample holder 300 in the imaging fluid.

[0065] Optionally, multiple hollow structures 10 are provided on the base 200 around the through hole 220, and multiple hollow structures 10 are provided on the sample holder 300 around the rotating shaft 310, in order to further save materials and reduce resistance during the adjustment process.

[0066] Optionally, combined Figure 2 , Figure 4 and Figure 5 As shown, the sample holder 300 is provided with slots 320, and the slots 320 are paired and matched. The light-transmitting clips 400 are inserted into the slots 320 one by one to ensure the installation stability of the light-transmitting clips 400.

[0067] Optionally, the light-transmitting clip 400 can be bonded by adding strong adhesive or UV adhesive to the slot 320 to improve the installation stability of the light-transmitting clip 400.

[0068] Optionally, the sample holder 300 can be configured with various specifications according to the shape and size of the sample. The slots 320 on each specification of the sample holder 300 have different spacing. The slots 320 can be connected to the hollow structure 10 or not connected to the hollow structure 10. The slots 320 with different spacing are adapted to hold samples of different specifications to meet different experimental needs.

[0069] Optionally, Figure 6 This is a utility model Figure 1 The structural diagram of the stent is as follows: Figure 6 As shown, the bracket 100 includes a first connecting plate 110 and a second connecting plate 120 that are connected to each other. The first connecting plate 110 and the second connecting plate 120 have different extension directions, for example, their extension directions are perpendicular to each other. The first connecting plate 110 is connected to the base 200 by means of plugging, bonding or other methods, and the second connecting plate 120 is located on the side of the first connecting plate 110 away from the base 200.

[0070] Optionally, the second connecting plate 120 is provided with magnet mounting holes, and positioning magnets 130 are embedded in the magnet mounting holes; several positioning magnets 130 can be provided, and the lines connecting several positioning magnets 130 can form a triangle. The positioning magnets 130 can magnetically fix the support 100 to the imaging platform of the light sheet microscope, thereby fixing the position of the sample holder assembly.

[0071] Example 2

[0072] Figure 7 This is an exploded view of the sample holder imaging component according to another embodiment of this utility model; Figure 8 yes Figure 7 Assembly diagram; Figure 9 This is a utility model Figure 7 A diagram of the support structure; Figure 10 This is a utility model Figure 7 The base structure diagram; the difference from Embodiment 1 is:

[0073] The support 100 includes a support body 140 and a boss 150. The support body 140 can be inserted into a slot of the imaging platform of a light sheet microscope. The base 200 includes a base body 201 and an extension 202 extending beyond the base body 201. The extension 202 is attached to the end face of the support body 140 by means of adhesive bonding or the like.

[0074] Along the length of the sample holder assembly, the extension 202 is positioned opposite the boss 150. The boss 150 serves as a reference for installation to ensure the correct relative position of the bracket 100 and the base 200 after assembly.

[0075] The angle between the end face of the bracket body 140 and the end face of the base 200 is 45°. One edge of the extension 202 can be provided with a chamfered surface 2021 to bond the end face of the bracket body 140 to the chamfered surface 2021 of the extension 202, thereby achieving a reliable connection between the two.

[0076] While the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make possible variations and modifications without departing from the spirit and scope of the present invention. Therefore, any modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention shall fall within the protection scope defined by the claims of the present invention.

Claims

1. A multi-directional imaging sample holder assembly for a light sheet microscope, characterized in that, include: A bracket (100) for mounting a light-sheet microscope; A base (200) is connected to the bracket (100), and the base (200) is provided with a plurality of limiting feet (210); The sample holder (300) is detachably connected to the base (200) so that the sample holder (300) can be repositioned 180° and then reinstalled into the installation area enclosed by the plurality of the limiting feet (210). A light-transmitting clip (400) is provided on the sample holder (300), the light-transmitting clips (400) are paired and arranged, and the area between the paired light-transmitting clips (400) is a sample holding area.

2. The sample holder assembly according to claim 1, characterized in that, include: The sample holder (300) has two different shooting angles.

3. The sample holder assembly according to claim 1, characterized in that, One of the base (200) and the sample holder (300) is provided with a through hole (220), and the other is provided with a rotating shaft (310) into which the through hole (220) is rotatably inserted.

4. The sample holder assembly according to claim 3, characterized in that, At least one of the base (200) and the sample holder (300) is provided with a hollow structure (10).

5. The sample holder assembly according to claim 4, characterized in that, Multiple hollow structures (10) are provided around the through hole (220), and / or multiple hollow structures (10) are provided around the rotating shaft (310).

6. The sample holder assembly according to claim 1, characterized in that, The sample holder (300) is provided with slots (320), the slots (320) are paired and arranged, and the light-transmitting clips (400) are inserted into the slots (320) one by one.

7. The sample holder assembly according to claim 6, characterized in that, The light-transmitting clip (400) is bonded to the slot (320).

8. The sample holder assembly according to claim 1, characterized in that, The bracket (100) includes a first connecting plate (110) and a second connecting plate (120) that are connected to each other, and the first connecting plate (110) and the second connecting plate (120) have different extending directions; The first connecting plate (110) is connected to the base (200).

9. The sample holder assembly according to claim 8, characterized in that, The second connecting plate (120) is provided with a positioning magnet (130).

10. The sample holder assembly according to claim 1, characterized in that, The bracket (100) includes a bracket body (140) and a boss (150), and the base (200) includes a base body (201) and an extension (202) extending beyond the base body (201), the extension (202) fitting the end face of the bracket body (140); Along the length of the sample holder assembly, the extension (202) and the boss (150) are arranged opposite to each other.

Images