Feed medium set mixing device

Abstract

The utility model relates to the field of bioengineering technology, propose a kind of feed medium suit mixed device, the present design includes discharging assembly, discharging assembly includes double bin seat, discharging assembly further includes the upper communication passage of being fixedly connected in symmetry at double bin seat bottom and being connected with double bin seat inner independent compartment, the mixing assembly that can be used to avoid depositing for mixing and stirring to be arranged in double bin seat below, be arranged in the opening and closing component of discharging assembly and mixing assembly connecting place can be opened and closed to control opening and closing of discharge;The present design adopts double bin independent storage and quantitative control and anti-deposition mixing process to solve the problem that material precision discharging cannot be controlled and mixed material is prone to deposit in prior art.

Description

Technical Field

[0001] This utility model relates to the field of bioengineering technology, specifically to a feeding culture medium kit mixing device. Background Technology

[0002] In biopharmaceutical and cell biology research, CHO cells have become important host cells for recombinant protein expression due to their ability to perform complex post-translational modifications and their excellent growth characteristics. However, during transient transfection of CHO cells, the basal medium often fails to provide sufficient and suitable nutrition throughout the cell culture process, leading to nutrient deficiency in the later stages of cell growth and limiting the expression of recombinant proteins. Existing fed-batch culture media have several problems. Some fed-batch media have unclear compositions and contain animal-derived components, which not only increases batch-to-batch variability but may also introduce the risk of pathogen contamination, affecting the quality of bioproducts. Furthermore, existing fed-batch media lack specificity for transient transfection expression in CHO cells, failing to accurately meet the nutritional needs of cells at different growth stages, thus limiting the expression efficiency and yield of recombinant proteins. Therefore, developing a well-defined, efficient, and highly targeted fed-batch culture medium kit for transient transfection of CHO cells is crucial. Utility Model Content

[0003] This invention proposes a feeding culture medium kit mixing device, which solves the problems of inability to control the precise feeding of materials and the easy precipitation of mixed materials in the prior art.

[0004] The technical solution of this utility model is as follows: a feeding culture medium kit mixing device, including a feeding component, the feeding component including a double-compartment base, the feeding component also including an upper connecting channel symmetrically fixedly connected to the bottom of the double-compartment base and connected to the independent compartments inside the double-compartment base, a mixing component disposed below the double-compartment base for stirring the mixture to avoid sedimentation, and an opening and closing component disposed at the connection between the feeding component and the mixing component for opening and closing to control the discharge.

[0005] Preferably, the feeding assembly further includes two sets of feeding ports, which are symmetrically fixedly connected to the top of the double-compartment base and communicate with the independent compartments inside the double-compartment base.

[0006] Preferably, the feeding assembly further includes a lower connecting channel, which is fixedly connected to the bottom of the upper connecting channel. The feeding assembly also includes a valve body, which is fixedly installed at the connection between the upper connecting channel and the lower connecting channel.

[0007] Preferably, the feeding assembly further includes a glass observation plate, which is fixedly connected to the through slot on the front of the lower channel. The feeding assembly also includes scale marks, which are evenly distributed on the front of the lower channel.

[0008] Preferably, the feeding assembly further includes a return pipe, one end of which is fixedly connected to the lower channel, and the other end of which is fixedly connected to the dual-compartment seat. The feeding assembly also includes a pump body, which is fixedly installed on the outside of the return pipe.

[0009] Preferably, the mixing assembly includes a mixing cylinder and a discharge pipe fixedly connected to the side of the mixing cylinder. The mixing cylinder is fixedly connected to the bottom of the lower channel. The mixing assembly also includes a drive motor, which is fixedly installed inside the mixing cylinder. The output end of the drive motor is fixedly connected to a main rotating shaft. The mixing assembly also includes a stirring plate, which is fixedly connected in a ring shape to the outside of the main rotating shaft.

[0010] Preferably, the opening and closing assembly includes a forward and reverse motor, which is fixedly installed on the top of the mixing cylinder, and a bidirectional threaded rod is fixedly connected to the output end of the forward and reverse motor.

[0011] Preferably, the opening and closing assembly further includes two sets of slides respectively threaded to opposite threads on both sides of the bidirectional threaded rod, a connecting rod fixedly connected to the side of the slide, and a closing plate fixedly connected to the end of the connecting rod away from the slide, the closing plate corresponding to the position of the lower channel.

[0012] The beneficial effects of this utility model are as follows:

[0013] I. Dual-warehouse independent storage and quantitative control

[0014] The dual-compartment design separates and stores two different culture media. Visual quantification is achieved through a glass observation plate and scale. Combined with the reflux pipe and pump body, the feed rate is dynamically adjusted, solving the problem of proportion error in traditional mixing.

[0015] II. Anti-sedimentation mixing design

[0016] The mixing component is directly integrated below the feeding channel. The drive motor drives the annular stirring plate to force mixing in the mixing cylinder, avoiding uneven mixing caused by the precipitation of high-concentration components.

[0017] III. Linked Opening and Closing Structure

[0018] The forward and reverse motors drive the bidirectional threaded rods to synchronously control two sets of closing plates, enabling the simultaneous opening and closing of the two channels. This ensures that the two culture media enter the mixing cylinder simultaneously, avoiding mixing delays. Attached Figure Description

[0019] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments.

[0020] Figure 1 This is a schematic diagram of the overall device of this utility model;

[0021] Figure 2This is a schematic diagram of the feeding assembly of this utility model;

[0022] Figure 3 This is a schematic diagram of the opening and closing component of this utility model;

[0023] Figure 4 This is a schematic diagram of the mixing component of this utility model;

[0024] In the diagram: 1. Feeding assembly; 11. Double-bin seat; 111. Feed inlet; 12. Upper connecting channel; 121. Valve body; 13. Lower connecting channel; 131. Glass observation plate; 132. Scale mark; 14. Return pipe; 141. Pump body; 2. Opening and closing assembly; 21. Forward and reverse motor; 211. Bidirectional threaded rod; 22. Slide; 221. Connecting rod; 23. Closing plate; 3. Mixing assembly; 31. Mixing cylinder; 311. Discharge pipe; 32. Drive motor; 33. Main shaft; 331. Mixing plate. Detailed Implementation

[0025] The technical solutions of this utility model will be clearly and completely described below with reference to the embodiments of this utility model. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this utility model.

[0026] Please see Figure 1 and Figure 2 and Figure 3 and Figure 4 This utility model provides a technical solution: a feeding culture medium kit mixing device, including a feeding component 1, the feeding component 1 including a double-compartment base 11, the feeding component 1 also including an upper connecting channel 12 symmetrically fixedly connected to the bottom of the double-compartment base 11 and connected to the independent compartments inside the double-compartment base 11, a mixing component 3 disposed below the double-compartment base 11 for stirring the mixture to avoid sedimentation, and an opening and closing component 2 disposed at the connection between the feeding component 1 and the mixing component 3 for opening and closing to control the discharge;

[0027] This design adopts a dual-compartment independent storage and quantitative control, as well as an anti-sedimentation mixing process, which solves the problems of inaccurate material feeding and easy sedimentation in existing technologies.

[0028] Please see Figure 2 The feeding assembly 1 also includes two sets of feeding ports 111, which are symmetrically fixedly connected to the top of the double-compartment base 11 and connected to the independent compartments inside the double-compartment base 11.

[0029] Transpro feed2 and Transpro feedB2 can be stored independently via dual-slot 11;

[0030] Please see Figure 2 The feeding assembly 1 also includes a lower connecting channel 13, which is fixedly connected to the bottom of the upper connecting channel 12. The feeding assembly 1 also includes a valve body 121, which is fixedly installed at the connection between the upper connecting channel 12 and the lower connecting channel 13.

[0031] By activating the valve body 121, Transpro feed2 and Transpro feedB2 located in the dual compartment 11 and the upper connecting channel 12 can fall into the lower connecting channel 13 respectively.

[0032] Please see Figure 2 The unloading assembly 1 also includes a glass observation plate 131, which is fixedly connected to the through slot on the front of the lower channel 13. The unloading assembly 1 also includes scale marks 132, which are evenly distributed on the front of the lower channel 13.

[0033] The output of Transpro feed2 and Transpro feedB2 in the lower channel 13 can be observed and compared through the glass observation plate 131 and the scale 132;

[0034] Please see Figure 2 The feeding assembly 1 also includes a return pipe 14, one end of which is fixedly connected to the lower connecting channel 13, and the other end of which is fixedly connected to the double chamber seat 11. The feeding assembly 1 also includes a pump body 141, which is fixedly installed on the outside of the return pipe 14.

[0035] When the output of Transpro feed2 and Transpro feedB2 in the lower channel 13 is lower than the predetermined value, it can be added by activating valve body 121. When the storage of Transpro feed2 and TransprofeedB2 in the lower channel 13 is higher than the predetermined value, pump body 141 can be activated to allow a portion of Transpro feed2 or Transpro feedB2 to flow back into the dual chamber seat 11 through their respective independent return pipes 14.

[0036] Please see Figure 3 and Figure 4 The mixing assembly 3 includes a mixing cylinder 31, a discharge pipe 311 fixedly connected to the side of the mixing cylinder 31, the mixing cylinder 31 fixedly connected to the bottom of the lower channel 13, the mixing assembly 3 also includes a drive motor 32, the drive motor 32 is fixedly installed inside the mixing cylinder 31, the output end of the drive motor 32 is fixedly connected to a main rotating shaft 33, the mixing assembly 3 also includes a stirring plate 331, the stirring plate 331 is fixedly connected to the outside of the main rotating shaft 33 in a ring shape;

[0037] The opening and closing assembly 2 includes a forward and reverse motor 21, which is fixedly installed on the top of the mixing cylinder 31. A bidirectional threaded rod 211 is fixedly connected to the output end of the forward and reverse motor 21.

[0038] The opening and closing assembly 2 also includes two sets of slides 22 that are threaded to opposite threads on both sides of the bidirectional threaded rod 211, a connecting rod 221 fixedly connected to the side of the slide 22, and a closing plate 23 fixedly connected to the end of the connecting rod 221 away from the slide 22. The closing plate 23 corresponds to the position of the lower channel 13.

[0039] After Transpro feed2 and Transpro feedB2 in the two sets of lower channels 13 have completed quantitative preparation, the forward and reverse motors 21 can be started to drive the bidirectional threaded rod 211 to rotate, thereby causing the slides 22 with the threaded connection at the opposite threads on both sides of the bidirectional threaded rod 211 to move synchronously towards each other. At this time, the connecting rods 221 fixedly connected to the sides of each slide 22 can drive the closing plate 23 to move synchronously towards each other. At this time, Transpro feed2 and Transpro feedB2 in each lower channel 13 fall into the mixing cylinder 31.

[0040] At this time, the drive motor 32 is started, which drives the main shaft 33 and the stirring plate 331 to rotate, thereby mixing the quantitative Transpro feed2 and Transpro feedB2. This design can avoid sedimentation between Transpro feed2 and Transpro feedB2. Finally, the mixed Transpro feed2 and Transpro feedB2 can be discharged by starting the discharge pipe 311.

[0041] Working principle:

[0042] First, quantitative Transpro feed2 and Transpro feedB2 are fed into the independent compartments of the dual-compartment 11 through the feed inlet 111 to complete the preparatory work.

[0043] Subsequently, by activating the valve body 121, Transpro feed2 and Transpro feedB2 located in the dual compartment 11 and the upper connecting channel 12 can fall into the lower connecting channel 13 respectively.

[0044] When the output of Transpro feed2 and Transpro feedB2 in the lower channel 13 is lower than the predetermined value, it can be added by activating valve body 121. When the storage of Transpro feed2 and TransprofeedB2 in the lower channel 13 is higher than the predetermined value, pump body 141 can be activated to allow a portion of Transpro feed2 or Transpro feedB2 to flow back into the dual chamber seat 11 through their respective independent return pipes 14.

[0045] After Transpro feed2 and Transpro feedB2 in the two sets of lower channels 13 have completed quantitative preparation, the forward and reverse motors 21 can be started to drive the bidirectional threaded rod 211 to rotate, thereby causing the slides 22 with the threaded connection at the opposite threads on both sides of the bidirectional threaded rod 211 to move synchronously towards each other. At this time, the connecting rods 221 fixedly connected to the sides of each slide 22 can drive the closing plate 23 to move synchronously towards each other. At this time, Transpro feed2 and Transpro feedB2 in each lower channel 13 fall into the mixing cylinder 31.

[0046] At this time, the drive motor 32 is started, which drives the main shaft 33 and the stirring plate 331 to rotate, thereby mixing the quantitative Transpro feed2 and Transpro feedB2. This design can avoid sedimentation between Transpro feed2 and Transpro feedB2. Finally, the mixed Transpro feed2 and Transpro feedB2 can be discharged by starting the discharge pipe 311.

[0047] The above are merely preferred embodiments of the present utility model and are not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. A feed culture medium mixing device, comprising a feeding assembly (1), characterized in that, The feeding assembly (1) includes a double-compartment base (11), and the feeding assembly (1) also includes an upper connecting channel (12) symmetrically fixedly connected to the bottom of the double-compartment base (11) and connected to the independent compartments inside the double-compartment base (11), a mixing assembly (3) provided below the double-compartment base (11) for mixing materials to avoid sedimentation, and an opening and closing assembly (2) provided at the connection between the feeding assembly (1) and the mixing assembly (3) for opening and closing to control the discharge.

2. The feed culture medium mixing device according to claim 1, characterized in that, The feeding assembly (1) also includes two sets of feeding ports (111), which are symmetrically fixedly connected to the top of the double-compartment base (11) and connected to the independent compartments inside the double-compartment base (11).

3. The supplemental culture medium mixing device according to claim 1, characterized in that, The feeding assembly (1) also includes a lower connecting channel (13), which is fixedly connected to the bottom of the upper connecting channel (12). The feeding assembly (1) also includes a valve body (121), which is fixedly installed at the connection between the upper connecting channel (12) and the lower connecting channel (13).

4. The feed culture medium mixing device according to claim 3, characterized in that, The feeding assembly (1) also includes a glass observation plate (131), which is fixedly connected to the through slot on the front of the lower channel (13). The feeding assembly (1) also includes a scale (132), which is evenly distributed on the front of the lower channel (13).

5. The feed culture medium mixing device according to claim 4, characterized in that, The feeding assembly (1) also includes a return pipe (14), one end of which is fixedly connected to the lower channel (13), and the other end of which is fixedly connected to the double chamber seat (11). The feeding assembly (1) also includes a pump body (141), which is fixedly installed on the outside of the return pipe (14).

6. The supplemental culture medium mixing device according to claim 3, characterized in that, The mixing assembly (3) includes a mixing cylinder (31) and a discharge pipe (311) fixedly connected to the side of the mixing cylinder (31). The mixing cylinder (31) is fixedly connected to the bottom of the lower channel (13). The mixing assembly (3) also includes a drive motor (32), which is fixedly installed inside the mixing cylinder (31). The output end of the drive motor (32) is fixedly connected to a main rotating shaft (33). The mixing assembly (3) also includes a stirring plate (331), which is fixedly connected in a ring shape to the outside of the main rotating shaft (33).

7. The supplemental culture medium mixing device according to claim 6, characterized in that, The opening and closing assembly (2) includes a forward and reverse motor (21), which is fixedly installed on the top of the mixing cylinder (31), and the output end of the forward and reverse motor (21) is fixedly connected to a bidirectional threaded rod (211).

8. A feed culture medium mixing device according to claim 7, characterized in that, The opening and closing assembly (2) also includes two sets of slides (22) that are threaded to opposite threads on both sides of the bidirectional threaded rod (211), a connecting rod (221) fixedly connected to the side of the slide (22), and a closing plate (23) fixedly connected to the end of the connecting rod (221) away from the slide (22). The closing plate (23) corresponds to the position of the lower channel (13).

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