Cell culture device with gas regulation function

Abstract

The application relates to the technical field of cell culture, and discloses a cell culture device with a gas adjusting function, which comprises a cell culture box, the top outer wall of the cell culture box is provided with a culture box pipeline, the outer wall of the culture box pipeline is fixedly connected with a connecting pipeline, the side outer wall of the connecting pipeline is fixedly connected with a mixing pipeline, the side outer wall of the mixing pipeline is fixedly connected with an air inlet pipe, the outer wall of a supporting plate is rotationally connected with a connecting shaft, the outer wall of the connecting shaft is fixedly connected with a primary turbofan, the outer wall of the connecting shaft is fixedly connected with a plurality of flat blades, the outer wall of the connecting shaft between the flat blades and the primary turbofan is fixedly connected with a secondary turbofan, and the inner wall of the mixing pipeline is provided with a spiral groove. In the utility model, the uniformity of gas mixing can be improved, the position with inconsistent regional concentration after gas entering the cell culture box can be avoided, the gas composition is highly consistent in the cell culture area, and a stable and uniform gas environment is provided for cell growth.

Description

Technical Field

[0001] This application relates to the field of cell culture technology, and in particular to a cell culture device with gas regulation function. Background Technology

[0002] Cell culture, as a key technology in the life sciences, is widely used in cutting-edge research such as biomedical development, disease model construction, cell therapy, and drug screening. In biomedical development, cell culture is used to mass-produce recombinant proteins, antibodies, and other biological products. When cells grow at high density, the demand for oxygen and nutrients surges, requiring extremely high stability of the gaseous environment. In disease model construction, cells are cultured to simulate the in vivo microenvironment, and the precise regulation of gas composition directly affects cell phenotype and function, which is crucial to the accuracy of disease research.

[0003] Regarding the aforementioned technologies, the inventors believe that most existing cell culture devices connect multiple gases to the air inlet of the cell culture device. However, the multiple gases are not fully mixed, which easily leads to inconsistent gas concentrations in cell regions when they enter the cell culture device. This results in significant differences in the growth status of cells in different locations due to the different gas compositions they receive. Therefore, a cell culture device with gas regulation function is proposed to solve the above problems.

[0004] The information disclosed in this background section is only intended to enhance the understanding of the background technology of this application, and therefore may include prior art that is not known to those skilled in the art. Utility Model Content

[0005] To address the aforementioned problems, this application provides a cell culture device with gas regulation function.

[0006] The cell culture device with gas regulation function provided in this application adopts the following technical solution:

[0007] A cell culture device with gas regulation function includes a cell culture chamber. The top outer wall of the cell culture chamber is provided with a culture chamber pipe for mixed gas to enter the cell culture chamber. A connecting pipe is fixedly connected to the outer wall of the culture chamber pipe. A mixing pipe is fixedly connected to the side outer wall of the connecting pipe. An air inlet pipe is fixedly connected to the side outer wall of the mixing pipe.

[0008] A support plate is fixedly connected to the inner wall of the intake pipe, and a connecting shaft is rotatably connected to the outer wall of the support plate. A primary turbofan is fixedly connected to the outer wall of one end of the connecting shaft, and multiple straight blades are fixedly connected to the outer wall of the other end of the connecting shaft. A secondary turbofan is fixedly connected to the outer wall of the connecting shaft between the straight blades and the primary turbofan. The secondary turbofan and the multiple straight blades are all located inside the mixing pipe. A spiral groove is formed on the inner wall of the mixing pipe outside the straight blades, and a heating component for easy control of gas temperature is provided on the outer side of the mixing pipe.

[0009] Preferably, the heating assembly includes a heating control device, a resistance wire, and a heating sleeve. The heating sleeve is disposed inside the mixing pipe, and the resistance wire is fixedly connected to the outside of the heating sleeve. The heating control device is fixedly connected to the upper surface of the cell culture chamber, and the heating control device is sleeved on the outside of the mixing pipe.

[0010] Preferably, a support is fixedly connected to the outer side wall of the cell culture chamber, and a four-way pipe is fixedly connected to the outer wall of the support. The four-way pipe is connected to the air inlet pipe through a flange, and the four-way pipe is connected to an external air source.

[0011] Preferably, the inlet diameter of the air intake pipe is larger than the outlet diameter.

[0012] Preferably, the cell culture chamber is equipped with a temperature sensor controller, which is electrically connected to the heating control device.

[0013] In summary, this application includes the following beneficial technical effects:

[0014] 1. This device accelerates gas flow through the tapered structure of the intake pipe, thereby driving the efficient rotation of the first-stage turbofan, which in turn drives the rotation of the second-stage turbofan and straight blades. In conjunction with the spiral grooves on the inner wall of the mixing pipe, it achieves multi-stage dynamic shearing, segmentation, and turbulence of the gas. Compared with the traditional simple open intake and reliance on natural gas diffusion mixing, this device can improve the uniformity of gas mixing, thereby achieving efficient mixing and avoiding inconsistent concentrations in different areas after the gas enters the cell culture chamber. This ensures that the gas composition is highly consistent throughout the cell culture area, providing a stable and uniform gas environment for cell growth.

[0015] 2. This device, through the installation of a resistance wire, heating jacket, and heating control device, controls the internal temperature of the cell culture chamber via a temperature sensor controller. This data is then fed back to an external controller, which adjusts the heating power of the resistance wire to preheat the mixed gas to a suitable temperature before it enters the cell culture chamber. This avoids the impact of temperature fluctuations on cell viability and significantly improves the success rate of cell culture. Attached Figure Description

[0016] Figure 1 This is an overall schematic diagram of an embodiment of the application;

[0017] Figure 2 This is a cross-sectional view of the heating control device structure according to the application embodiment;

[0018] Figure 3 This is a cross-sectional view of the hybrid pipeline structure according to an embodiment of the application;

[0019] Figure 4 This is a cross-sectional view of the intake pipe structure according to the application embodiment;

[0020] Figure 5 This is a schematic diagram of the inside of the cell culture incubator in the embodiment of the application;

[0021] Figure 6 for Figure 1 Enlarged schematic diagram of the structure at point A in the middle.

[0022] Explanation of reference numerals in the attached diagram: 1. Cell culture incubator; 2. Incubator tubing; 3. Connecting tubing; 4. Heating control device; 5. Mixing tubing; 6. Air inlet pipe; 7. Support; 8. Four-way pipe; 9. Resistance wire; 10. Heating jacket; 11. Spiral groove; 12. Connecting shaft; 13. Straight blade; 14. Secondary turbofan; 15. Support plate; 16. Primary turbofan; 17. Temperature sensor controller. Detailed Implementation

[0023] The following is in conjunction with the appendix Figure 1 - Figure 6 This application will be described in further detail.

[0024] A cell culture device with gas regulation function includes a cell culture chamber 1. The top outer wall of the cell culture chamber 1 is provided with a culture chamber pipe 2 for mixed gas to enter the cell culture chamber 1. The culture chamber pipe 2 is provided with multiple gas nozzles connected to the culture chamber pipe 2. When the mixed gas enters the cell culture chamber 1 through the culture chamber pipe 2, it is sprayed out through the multiple gas nozzles to complete the air environment required for cell culture. A connecting pipe 3 is fixedly connected to the outer wall of the culture chamber pipe 2. A mixing pipe 5 is fixedly connected to the side outer wall of the connecting pipe 3. A gas concentration sensor is provided on the inner wall of the connecting pipe 3 near the culture chamber pipe 2 to detect the concentration of gas components in real time and transmit the data to an external controller to control the flow rate of various gases. An air inlet pipe 6 is fixedly connected to the side outer wall of the mixing pipe 5. The culture chamber pipe 2 is connected to the mixing pipe 5 through the connecting pipe 3, and the mixing pipe 5 is fixedly connected to the air inlet pipe 6 to form a complete gas delivery path, ensuring that the gas can be transmitted from the external gas source to the cell culture chamber 1 in an orderly and stable manner to provide the gas environment required for cell culture.

[0025] A support plate 15 is fixedly connected to the inner wall of the intake pipe 6. A connecting shaft 12 is rotatably connected to the outer wall of the support plate 15. A first-stage turbofan 16 is fixedly connected to the outer wall of one end of the connecting shaft 12. The blades of the first-stage turbofan 16 are helical, and the helical direction of the blades is consistent with the gas flow direction. The first-stage turbofan 16 can more effectively convert the kinetic energy of the gas into rotational mechanical energy. Multiple straight blades 13 are fixedly connected to the outer wall of the other end of the connecting shaft 12. A second-stage turbofan 14 is fixedly connected to the outer wall of the connecting shaft 12 between the straight blades 13 and the first-stage turbofan 16. The blades of the second-stage turbofan 14 are inclined, and the inclination angle is 30-60 degrees. When rotating, it generates radial and axial shear forces on the gas, thereby improving the gas mixing effect. The second-stage turbofan 14 and the multiple straight blades 13 are all located in the mixing pipe 5. Inside the mixing pipe 5, the rotation of the primary turbofan 16 drives the secondary turbofan 14 and multiple straight blades 13 to rotate. The inner wall of the mixing pipe 5, located outside the straight blades 13, has a spiral groove 11. The radial and axial shear forces generated by the inclined blades of the secondary turbofan 14 can quickly disperse different gases and initially mix them. The spiral angle of the spiral groove 11 is 45-75 degrees. During the rotation of the straight blades 13, they cooperate with the spiral groove 11 to divide and turbulent the gas, further enhancing the gas mixing effect and avoiding differences in cell growth status due to uneven gas composition, thus ensuring the reliability of cell culture experimental results. In addition, a heating component is provided on the outside of the mixing pipe 5 to facilitate the control of gas temperature, making it meet the requirements of cell culture.

[0026] The heating assembly includes a heating control device 4, a resistance wire 9, and a heating jacket 10. The heating jacket 10 is disposed inside the mixing pipe 5, and the resistance wire 9 is fixedly connected to the outside of the heating jacket 10. The heating control device 4 is fixedly connected to the upper surface of the cell culture chamber 1. The heating control device 4 includes a temperature regulation program, a power supply, and a temperature sensor. The temperature sensor is used to monitor the temperature of the gas inside the heating jacket 10. The heating control device 4 adjusts the current of the resistance wire 9 through the temperature regulation module, thereby changing its heating power. The power supply ensures a stable power supply to the heating assembly. It should be noted that the temperature regulation program, power supply, resistance wire 9, and temperature sensor all adopt corresponding structures in the prior art, and the combination of the above structures also adopts the prior art. It should be further noted that when the temperature sensor detects that the gas temperature is lower than the required temperature inside the cell culture phase, the temperature regulation program controls the resistance wire 9 to increase the power so that the gas temperature inside the heating jacket 10 rises. The heating control device 4 is sleeved on the outside of the mixing pipe 5, and controls the temperature inside the heating jacket 10 by heating the resistance wire 9, so that the internal gas maintains a certain temperature.

[0027] A support 7 is fixedly connected to the outer side wall of the cell culture incubator 1, and a four-way pipe 8 is fixedly connected to the outer wall of the support 7. The four-way pipe 8 is connected to the air inlet pipe 6 through a flange, and the four-way pipe 8 is connected to an external air source. A one-way valve is installed on the pipes of the four-way pipe 8 connected to each external air source to prevent gas backflow. At the same time, a gas flow control valve is installed on the pipes between the four-way pipe 8 and various gas supply tanks to control the gas flow rate. The gas flow control valve adopts the corresponding structure in the prior art, and is equipped with a power supply and controller so that the user can control the opening and closing of the gas flow valve according to the needs, thereby achieving the effect of controlling different gas injection devices.

[0028] The inlet diameter of the intake pipe 6 is larger than the outlet diameter, forming a tapered structure that increases the flow velocity of the gas after entering the intake pipe 6, thereby better driving the first-stage turbofan 16 to rotate.

[0029] The cell culture chamber 1 is equipped with a temperature sensor controller 17, which is electrically connected to the heating control device 4. The temperature sensor controller 17 monitors the temperature inside the cell culture chamber 1 in real time and transmits the temperature signal to the heating control device 4. The heating control device 4 controls the heating power of the resistance wire 9 according to the received temperature signal, thereby achieving precise control of the temperature inside the cell culture chamber 1.

[0030] The implementation principle of a cell culture device with gas regulation function according to an embodiment of this application is as follows: First, multiple external gas supply tanks are supplied with gas through pipes connected to a four-way pipe 8. One-way valves on the pipes 3 connecting the four-way pipe 8 to each external gas source prevent gas backflow, ensuring that gas can only flow in one direction. Simultaneously, a gas flow control valve installed in the pipe between the four-way pipe 8 and the gas supply tank can initially adjust the flow rate of each gas entering the inlet pipe 6 according to the preset requirements of cell culture, providing a basic proportion of gas raw materials for subsequent gas mixing.

[0031] After flow regulation, the gas enters the intake pipe 6. Since the inlet diameter of the intake pipe 6 is larger than the outlet diameter, the gas velocity is increased. This increases the kinetic energy of the gas, driving the first-stage turbofan 16 to rotate. The spiral direction of the first-stage turbofan 16 is the same as the gas flow, converting the gas kinetic energy into mechanical energy. The first-stage turbofan 16 drives the second-stage turbofan 14 and the straight blades 13 to rotate synchronously via the connecting shaft 12. When the second-stage turbofan 14 rotates, it generates radial and axial shear forces on the gas, rapidly dispersing different gases and achieving initial mixing. During rotation, the straight blades 13 engage with the spiral grooves 11 in the mixing pipe 5, further segmenting and turbulent the gas, increasing the mixing effect and ensuring thorough mixing of multiple gases in the mixing pipe 5.

[0032] During this process, the heating component outside the mixing pipe 5 regulates the temperature of the mixed gas, enabling the temperature sensor controller 17 inside the cell culture chamber 1 to monitor the temperature in real time and transmit the temperature signal to the heating control device 4. The temperature regulation module in the heating control device 4 adjusts the current of the resistance wire 9 according to the received temperature signal, thereby changing its heating power. The resistance wire 9 is wound around the outside of the heating jacket 10, which heats the gas inside the mixing pipe 5. The power control module ensures a stable power supply to the heating component, thus controlling the temperature of the mixed gas within the appropriate range required for cell culture, preventing subsequent gas intake from disrupting the original temperature environment inside the culture chamber.

[0033] The gas concentration sensor on the connecting pipe 3 detects the components in the mixed gas, enabling the controller to adjust the flow rate of various gases through the control algorithm. When the mixed gas enters the cell culture chamber 1 through the incubator pipe 2, multiple gas nozzles inside the incubator pipe 2 spray the mixed gas evenly, forming a stable and suitable air environment in the cell culture chamber 1, providing the required gas conditions for cell growth.

Claims

1. A cell culture device with gas regulation function, comprising a cell culture incubator (1), characterized in that: The top outer wall of the cell culture box (1) is provided with a culture box pipe (2) for the mixed gas to enter the cell culture box (1). The outer wall of the culture box pipe (2) is fixedly connected to a connecting pipe (3). The outer wall of the connecting pipe (3) is fixedly connected to a mixing pipe (5). The outer wall of the mixing pipe (5) is fixedly connected to an air inlet pipe (6). The inner wall of the intake pipe (6) is fixedly connected to a support plate (15), and the outer wall of the support plate (15) is rotatably connected to a connecting shaft (12). One end of the connecting shaft (12) is fixedly connected to a first-stage turbofan (16), and the other end of the connecting shaft (12) is fixedly connected to a plurality of straight blades (13). The outer wall of the connecting shaft (12) between the straight blades (13) and the first-stage turbofan (16) is fixedly connected to a second-stage turbofan (14). The second-stage turbofan (14) and the plurality of straight blades (13) are all located inside the mixing pipe (5). The inner wall of the mixing pipe (5) located outside the straight blades (13) is provided with a spiral groove (11), and a heating component for easy control of gas temperature is provided on the outer side of the mixing pipe (5).

2. The cell culture device with gas regulation function according to claim 1, characterized in that: The heating assembly includes a heating control device (4), a resistance wire (9), and a heating sleeve (10). The heating sleeve (10) is disposed inside the mixing pipe (5), and the resistance wire (9) is fixedly connected to the outside of the heating sleeve (10). The heating control device (4) is fixedly connected to the upper surface of the cell culture box (1), and the heating control device (4) is sleeved on the outside of the mixing pipe (5).

3. The cell culture device with gas regulation function according to claim 1, characterized in that: The cell culture box (1) is fixedly connected to a support (7) on its outer side wall, and a four-way pipe (8) is fixedly connected to the outer wall of the support (7). The four-way pipe (8) is connected to the air inlet pipe (6) through a flange, and the four-way pipe (8) is connected to an external air source.

4. A cell culture device with gas regulation function according to claim 1, characterized in that: The inlet diameter of the air intake pipe (6) is larger than the outlet diameter.

5. A cell culture device with gas regulation function according to claim 1, characterized in that: The cell culture chamber (1) is equipped with a temperature sensor controller (17), which is electrically connected to the heating control device (4).

Images