Ion source, ion mobility spectrometry based microbial identification device

Abstract

The utility model relates to microorganism identification technical field, especially in kind of ion source, microorganism identification device based on ion migration spectrum, including shell and setting in the sample target plate, XY moving platform, laser assembly, camera subassembly, leading electrode and ion sampler in the shell, sample target plate is installed on the bottom plate of shell through XY moving platform, and laser assembly and camera subassembly set up in the top of sample target plate, and laser assembly is arranged in the orthogonal with sample target plate, and leading electrode sets up between sample target plate and laser assembly, and ion sampler sets up between sample target plate and leading electrode, and ion sampler is arranged in parallel with sample target plate, and the inside of shell is atmospheric pressure environment, the utility model provides a kind of structure design simple, compact, ionization efficiency higher, ion beam is more concentrated, low in cost, stability and security are high, and can quickly, accurately realize the ion source, microorganism identification device based on ion migration spectrum of microorganism identification analysis.

Description

Technical Field

[0001] This utility model relates to the field of microbial identification technology, and in particular to an ion source and a microbial identification device based on ion mobility spectrometry. Background Technology

[0002] Microbial identification is a core technology for precision treatment of clinical infections (such as rapid identification of sepsis pathogens), monitoring of industrial fermentation processes (such as probiotic activity assessment), and environmental microbial tracing (such as detection of water pollution indicator bacteria). Commonly used microbial identification methods include phenotype-based biochemical identification methods and gene sequencing technology. Both have significant limitations: phenotype-based biochemical identification methods usually take 18-72 hours to complete and have low accuracy; gene sequencing technology has a complex operation process, high equipment costs, and is difficult to achieve routine application.

[0003] Currently, identification methods based on matrix-assisted laser desorption / ionization time-of-flight mass spectrometry (MALDI-TOF MS) are widely used. This method involves bombarding a co-crystallized microorganism and matrix with a laser, ionizing protein (or peptide) molecules. The ions are then accelerated by a high-voltage electric field (typically 20 kV) and enter a time-of-flight mass analyzer. The analyzer acts like an ion racetrack; ions with different mass-to-charge ratios have different velocities (smaller mass-to-charge ratio ions achieve higher velocities, and larger mass-to-charge ratio ions achieve relatively lower velocities), resulting in different arrival times for the ions at the detector. This allows for the separation of different proteins (or peptides). To ensure that ions do not collide and scatter during flight, the system needs to maintain a temperature less than 10 °C. -6The high vacuum environment of mbar ensures that the mean free path is much greater than the drift tube length. Since the proteins (or peptides) of microorganisms are conserved, the protein fingerprint spectra of different species have fingerprint characteristics. Therefore, the species identification can be completed by comparing the fingerprint spectra formed by matrix-assisted laser desorption / ionization time-of-flight mass spectra with the established microbial protein fingerprint spectrum database (such as the Bruker MBT library). However, this technology also has the following significant limitations: (1) The high vacuum environment is expensive to build, and continuous operation leads to high energy consumption, requiring frequent maintenance to ensure vacuum stability; (2) Due to the poor ion utilization rate under high vacuum environment, the core detection module relies on precision devices such as microchannel plates (MCP) or electron multiplier tubes, which are costly; (3) In order to achieve efficient acceleration and precise focusing of ions, the system must construct a high voltage electric field of tens of thousands of volts and integrate a delay extraction device with nanosecond precision (because there is obvious velocity dispersion after laser ionization, it is necessary to use delay extraction technology to make the ion flow more efficiently). (4) When the laser equipment bombards the co-crystallization of microorganisms and matrix, in order to avoid the blocking of the laser beam and achieve the transmission of the ion beam, the laser beam is irradiated at an inclined angle to achieve ionization, resulting in a large laser spot focusing area and uneven laser spot energy distribution, which leads to the situation that some particles are not ionized; moreover, the dispersion angle of the ion beam generated by the bombardment is uncertain, which will affect the accuracy and stability of subsequent identification and analysis.

[0004] The combination of these multiple technical bottlenecks results in high purchase and maintenance costs for the entire set of equipment, as well as poor accuracy and stability in the identification and analysis. Utility Model Content

[0005] The technical problem to be solved by this utility model is to overcome the shortcomings of the existing technology and provide an ion source and a microbial identification device based on ion mobility spectrum that has a simple and compact structure, higher ionization efficiency, more concentrated ion beam, low cost, high stability and safety, and can quickly and accurately realize microbial identification and analysis.

[0006] The technical solution adopted by this utility model to solve its technical problem is: an ion source, including a shell and a sample target plate, an XY stage, a laser component, a camera component, an extraction electrode, and an ion sampler disposed inside the shell; the sample target plate is mounted on the bottom plate of the shell via the XY stage, the laser component and the camera component are disposed above the sample target plate, the laser component is orthogonally arranged to the sample target plate, the extraction electrode is disposed between the sample target plate and the laser component, and the ion sampler is disposed between the sample target plate and the extraction electrode, and the ion sampler is arranged parallel to the sample target plate; the interior of the shell is an atmospheric pressure environment.

[0007] Furthermore, it also includes an auxiliary gas line and a heating assembly; the auxiliary gas line is disposed between the sample target plate and the extraction electrode, and the auxiliary gas line is disposed opposite to the ion sampler, and the heating assembly is mounted on the ion sampler.

[0008] Furthermore, the laser assembly includes a laser source, a laser collimating lens, and a laser focusing lens; the laser source, laser collimating lens, and laser focusing lens are arranged sequentially from top to bottom, and the laser source is mounted on the top plate of the housing.

[0009] Furthermore, the camera assembly includes a camera and a camera lens; the camera is mounted on the top plate of the housing and is positioned on one side of the laser light source, and the camera lens is positioned between the sample target plate and the camera.

[0010] Furthermore, the ion sampler is a capillary or a sampling cone.

[0011] A microbial identification device based on ion mobility spectrometry includes the aforementioned ion source, ion funnel, ion gate, traveling wave migration separation zone, detector, and data analysis module. The ion source is connected to the traveling wave migration separation zone via the ion funnel. The ion gate is located at the outlet of the ion funnel and is used to control the entry of ions into the traveling wave migration separation zone. The detector is installed on the side of the traveling wave migration separation zone away from the ion funnel and is communicatively connected to the data analysis module. The ion funnel is sealed within a chamber, and the chamber is connected to a vacuum device.

[0012] Furthermore, the traveling wave migration separation region includes an ion transport channel, the inner wall of which is attached to a flexible circuit board.

[0013] Furthermore, multiple parallel electrodes are arranged on the flexible circuit board.

[0014] Furthermore, the cross-section of the ion transport channel is circular or square.

[0015] The beneficial effects of this utility model are:

[0016] (1) By orthogonally setting the laser component and the sample target plate, the laser beam bombards the sample on the sample target plate perpendicularly, resulting in a more uniform energy distribution of the laser spot, better spot focusing effect, and stronger energy. This generates a large number of ions along the laser beam direction, forming an ion beam with higher ionization efficiency and more concentrated ion beam, reducing ion divergence and thus significantly improving the accuracy and stability of subsequent identification and analysis. At the same time, the parallel setting of the ion sampler and the sample target plate makes the axis of the ion sampler perpendicular to the ion beam, ultimately achieving efficient and stable ion capture.

[0017] (2) This invention employs ion mobility spectrometry for the identification and analysis of microorganisms. Since ion mobility spectrometry achieves separation through differences in ion mobility in an electric field, it offers the following advantages compared to conventional matrix-assisted laser desorption / ionization time-of-flight mass spectrometry: it operates under ambient pressure / low vacuum, eliminating the need for a high-vacuum environment and thus eliminating the need for high-vacuum equipment such as molecular pumps, significantly reducing equipment complexity and energy consumption; it also boasts high ion utilization, eliminating the need for precision components such as microchannel plates or electron multiplier tubes, significantly reducing costs and extending service life; and it eliminates the need for high-voltage power supplies of tens of thousands of volts for ion extraction and acceleration, increasing stability and safety, and further reducing costs. Reduced costs; ion switching control can be achieved through ion gates, eliminating the need for nanosecond-level pulse delay extraction technology, significantly reducing device costs, lowering instrument failure rates, increasing device stability, and reducing maintenance costs; the collision cross-section information provided by ion mobility spectrometry can more accurately analyze sample structural information, providing structural dimension analysis for drug resistance gene expression products, virulence factors, etc., forming an effective supplement to protein quality fingerprints; the compact structural design supports real-time on-site detection, enabling rapid and accurate identification and analysis of microorganisms, and exhibiting higher sensitivity to small molecules such as metabolites and lipids. Attached Figure Description

[0018] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0019] Figure 1 This is a cross-sectional view of the ion source in this utility model;

[0020] Figure 2 This is a cross-sectional view of the microbial identification device based on ion mobility spectrometry in this utility model;

[0021] Figure 3 This is a diagram showing the unfolded state of the flexible circuit board in this utility model;

[0022] Figure 4 This is a diagram showing the bending state of the flexible circuit board in this utility model;

[0023] Figure 5 It is the ion migration spectrum of Escherichia coli;

[0024] Figure 6 It is the ion migration spectrum of Staphylococcus aureus;

[0025] Figure 7 This is the ion migration spectrum of Acinetobacter baumannii.

[0026] In the diagram: 110, outer shell; 120, sample target plate; 130, XY stage; 140, laser assembly; 141, laser source; 142, laser collimating lens; 143, laser focusing lens; 150, camera assembly; 151, camera; 152, camera lens; 160, extraction electrode; 170, ion sampler; 180, auxiliary gas line; 190, heating assembly; 200, ion funnel; 300, ion gate; 400, traveling wave migration separation zone; 410, ion transport channel; 500, detector. Detailed Implementation

[0027] The present invention will now be further described in conjunction with the accompanying drawings and preferred embodiments. These drawings are simplified schematic diagrams, illustrating only the basic structure of the present invention, and therefore only show the components relevant to the present invention.

[0028] Example 1

[0029] like Figure 1 As shown, an ion source includes a housing 110 and a sample target plate 120, an XY stage 130, a laser assembly 140, a camera assembly 150, an extraction electrode 160, and an ion sampler 170 disposed within the housing 110. The sample target plate 120 is mounted on the bottom plate of the housing 110 via the XY stage 130. The laser assembly 140 and the camera assembly 150 are disposed above the sample target plate 120 and orthogonally to the sample target plate 120. The extraction electrode 160 is disposed between the sample target plate 120 and the laser assembly 140. The ion sampler 170 is disposed between the sample target plate 120 and the extraction electrode 160 and is parallel to the sample target plate 120. The interior of the housing 110 is an atmospheric pressure environment.

[0030] By orthogonally setting the laser component 140 to the sample target plate 120, the laser beam bombards the sample on the sample target plate 120 perpendicularly. This results in a more uniform energy distribution of the laser spot, better spot focusing, and stronger energy, generating a large number of ions along the laser beam direction to form an ion beam. This leads to higher ionization efficiency, a more concentrated ion beam, and reduced ion divergence, thereby significantly improving the accuracy and stability of subsequent identification and analysis. Simultaneously, the parallel setting of the ion sampler 170 to the sample target plate 120 ensures that the axis of the ion sampler 170 is perpendicular to the ion beam, ultimately achieving efficient and stable ion capture.

[0031] Specifically, the sample target plate 120 has multiple sample target points distributed on it for placing samples. The sample target plate 120 is made of stainless steel. The XY stage 130 drives the sample target plate 120 to perform two-dimensional motion in the X and Y directions. The XY stage 130 adopts existing technology. The extraction electrode 160 adopts a ring design to avoid the laser beam emitted by the laser component 140. The extraction electrode 160 is arranged parallel to the sample target plate 120, and directly utilizes the high-voltage electric field formed by the ion sampler 170 and the sample target plate 120. In comparison, the electric field is more uniform, which is beneficial for optimizing the ion extraction path and reducing ion kinetic energy dispersion. The right end of the ion sampler 170 extends from the right side plate of the outer shell 110. The ion sampler 170 adopts a capillary or sampling cone. The capillary is made of stainless steel with an inner diameter of 0.3-0.5 mm and a length of 50-200 mm. A bias voltage (usually 300V) can be applied inside the ion sampler 170 to form a traction electric field, which allows ions to be transported more orderly inside the ion sampler 170.

[0032] like Figure 1 As shown, the ion source also includes an auxiliary gas line 180 and a heating assembly 190; the auxiliary gas line 180 is disposed between the sample target plate 120 and the extraction electrode 160, and is disposed opposite to the ion sampler 170, and the heating assembly 190 is mounted on the ion sampler 170. Specifically, the auxiliary gas line 180 is mounted on the left side plate of the housing 110.

[0033] The auxiliary gas line 180 applies a dry, heated auxiliary gas (such as nitrogen). This auxiliary gas collides with the ion beam generated by the laser beam bombardment, helping the ions to quickly enter a stable state. Simultaneously, the auxiliary gas has a certain pressure, which guides the ions, helping the ion beam to converge and flow towards the ion sampler 170. The heating assembly 190 heats the ion sampler 170 (to 150-250°C), helping to eliminate ion clustering effects and prevent blockages in the ion sampler 170.

[0034] like Figure 1 As shown, the laser assembly 140 includes a laser source 141, a laser collimating lens 142, and a laser focusing lens 143. The laser source 141, laser collimating lens 142, and laser focusing lens 143 are arranged sequentially from top to bottom, with the laser source 141 mounted on the top plate of the housing 110. Specifically, the laser energy of the laser source 141 is controlled at the μJ level to avoid excessive fragmentation; the laser collimating lens 142 is 15mm away from the end face of the laser source 141, and its focal length is 15mm, used to achieve laser beam collimation; the laser focusing lens 143 is 20mm away from the laser collimating lens 142, and its focal length is 25mm. The laser focusing lens 143 is made of ultraviolet fused silica material, with an ultraviolet anti-reflection coating on its surface.

[0035] like Figure 1 As shown, the imaging assembly 150 includes a camera 151 and an imaging lens 152. The camera 151 is mounted on the top plate of the housing 110 and is positioned to one side of the laser light source 141. The imaging lens 152 is positioned between the sample target plate 120 and the camera 151. The imaging assembly 150 is configured to image the target points on the sample target plate 120, facilitating observation of sample distribution and ionization.

[0036] Example 2

[0037] like Figures 2-4 As shown, a microbial identification device based on ion mobility spectrometry includes an ion source, an ion funnel 200, an ion gate 300, a traveling wave migration separation zone 400, a detector 500, and a data analysis module as described in Example 1. The ion source is connected to the traveling wave migration separation zone 400 through the ion funnel 200. The ion gate 300 is located at the outlet of the ion funnel 200 and is used to control the entry of ions into the traveling wave migration separation zone 400. The detector 500 is installed on the side of the traveling wave migration separation zone 400 away from the ion funnel 200. The detector 500 is communicatively connected to the data analysis module. The ion funnel 200 is sealed in a chamber, and the chamber is connected to a vacuum device.

[0038] Ion mobility spectrometry (IMS) is used for the identification and analysis of microorganisms. Since IMS separates ions based on differences in their migration rates in an electric field, it offers several advantages over conventional matrix-assisted laser desorption / ionization time-of-flight mass spectrometry (MAMS): it operates under ambient pressure / low vacuum, eliminating the need for a high-vacuum environment and high-vacuum equipment such as molecular pumps, significantly reducing equipment complexity and energy consumption; it also boasts higher ion utilization, eliminating the need for precision components like microchannel plates or electron multiplier tubes, resulting in significantly lower costs and longer lifespan; and it eliminates the need for high-voltage power supplies (tens of thousands of volts) for ion extraction and acceleration, increasing stability and safety, further reducing costs. Ion switching control can be achieved through an ion gate of 300, eliminating the need for nanosecond-level pulse delay extraction technology, which greatly reduces the cost of the device, lowers the failure rate of the instrument, increases the stability of the device, and reduces maintenance costs. The collision cross section (CCS) information provided by ion mobility spectrometry can more accurately analyze the structural information of samples, providing structural dimension analysis for drug resistance gene expression products, virulence factors, etc., forming an effective supplement to protein quality fingerprints. The compact structural design supports real-time on-site detection, enabling rapid and accurate identification and analysis of microorganisms, and has higher sensitivity to small molecules such as metabolites and lipids.

[0039] Specifically, the ion source can also employ electrospray ionization (ESI), as long as it can achieve soft ionization of microbial proteins, maintain the integrity of the molecular structure to the maximum extent, and avoid generating fragment ions. The right end of the ion sampler 170 in the ion source extends into the ion funnel 200. The ion funnel 200 consists of 50-100 stainless steel plates with a plate spacing of 1-2 mm. A 1-2 MHz radio frequency voltage (100-200 Vpp) and an axial DC gradient (200 V at the inlet to 50 V at the outlet) are applied. The radio frequency field generates a pseudo-potential trap to constrain the radial movement of ions, while the DC gradient promotes axial ion transport, ultimately forming an ion beam with a diameter less than 1 mm at the outlet. The traveling wave migration separation region 400 includes an ion transport channel 410, the inner wall of which is attached to a flexible circuit board. The cross-section of the ion transport channel 410 is circular or square, and the ion transport channel 410 uses a metal barrel. Multiple parallel electrodes (such as...) are arranged on the flexible circuit board. Figure 3 and Figure 4 In the shaded area, parallel electrodes are 1 mm wide and 1 mm apart. Ten parallel electrodes form a group, and the corresponding electrodes in each group are connected in parallel. The ion funnel 200 and the traveling wave migration separation region 400 are both in a vacuum environment. The detector 500 is a Faraday cup detector.

[0040] Since the ion source is under atmospheric pressure and the ion funnel 200 is under vacuum, a pressure difference is created between the two ends of the ion sampler 170. The directional gas flow of the pressure field is more conducive to the efficient transport of ions, making the ion transport more stable, thereby improving the accuracy of the analysis and reducing the complexity and cost of the device.

[0041] During operation, microorganisms are coated onto the sample target plate 120 in the ion source, and an α-cyano-4-hydroxycinnamic acid composite (CHCA) matrix is ​​applied to the surface to form a co-crystallization of microorganisms and matrix. Under atmospheric pressure, pulsed ultraviolet laser (such as a 337nm nitrogen laser) emitted from the laser source 141 in the laser component 140 vertically bombards the co-crystallization, causing desorption ionization, which allows the microorganisms and matrix molecules to enter the gaseous state and undergo energy and charge transfer. A negative high voltage (-2000 to -5000V) is applied to the extraction electrode 160. The applied negative high voltage is eliminated after a certain delay (usually less than 50 microseconds) after the laser pulse, and the ions are removed from the sample by the action of the high voltage electric field. After the high-voltage electric field is eliminated, due to the pressure difference across the ion sampler 170 (the vacuum equipment keeps the chamber at a low vacuum), the ion beam enters the ion sampler 170 under the influence of the airflow. During the ionization stage, the sample target plate 120 is grounded, making the electric field distribution above the sample target plate 120 more uniform and preventing edge effects. This avoids the ion collision loss at the end face and outer wall of the ion sampler 170 caused by edge field effects under the action of the high-voltage electric field, as well as the directional loss under the guidance of the strong electric field. The ion gate 300 controls the ion implantation traveling wave migration separation region 400 in an orderly manner through periodic voltage switching: when closed, a high voltage (100-300V) is applied to block the ion flow, and when open... The pulsed injection method, briefly switching to zero voltage release of discrete ion packets (10-200 μs), ensures microsecond-level accuracy in migration time measurement by strictly synchronizing the pulsed injection with the electric field of the traveling wave migration separation zone 400 (error less than 1 μs). This eliminates signal overlap and provides a clear time starting point for ion migration spectra, ultimately improving resolution and quantitative accuracy. After entering the traveling wave migration separation zone 400, a periodically varying traveling wave voltage (wave height 10-100 V, wave velocity 10-100 m / s) is applied to each set of parallel electrodes under a 1-10 Torr nitrogen atmosphere, causing ion directional movement and separation. Under the combined action of the traveling wave electric field and the buffer gas, different ions migrate according to their mobility. The ions move at different speeds: compact ions have small collision cross-sections and migrate quickly, while loosely structured ions collide frequently and migrate slowly. They ultimately reach detector 500 in order of migration rate, forming time-separated ion migration spectra, achieving ion separation based on collision cross-sections. Detector 500 detects the ion signal and converts it into an electrical signal. This signal is then digitized by a transimpedance amplifier and an ADC to generate the ion migration spectrum. Detector 500 transmits the generated ion migration spectrum to the data analysis module. The data analysis module performs similarity matching between the ion migration spectrum and a reference spectrum in a spectral database, or uses a machine learning model to perform pattern recognition on the processed ion migration spectrum, thereby completing the identification and analysis. The spectral database is an existing ion migration spectrum database; the machine learning model is existing technology.

[0042] It should be noted that the sources of microorganisms include single colonies isolated from solid culture media (obtained by streak plating or spread plating), microbial communities purified by centrifugation (usually 3000-10000g, 5-15 minutes) or membrane filtration after culturing in liquid culture media, microorganisms specifically captured using functionalized magnetic beads (such as magnetic beads with broad-spectrum antibody-modified surfaces), and complex microbial communities in their natural state (such as environmental samples or the human microbiome); biomass must be strictly controlled during sampling (usually 10). 3 -10 7 CFU (Continuous Fusion Function) is used to ensure the stability of subsequent mass spectrometry signals.

[0043] This application fully leverages the operational advantages of the ion source operating under atmospheric pressure and the high-throughput separation capability of the 400-degree traveling wave migration separation region. It is suitable for clinical pathogen detection, environmental microbial monitoring, and other fields, and features simple operation, low cost, and convenient maintenance. The ion mobility spectra obtained using this application for the identification of Escherichia coli, Staphylococcus aureus, and Acinetobacter baumannii are shown below. Figures 5-7 .

[0044] Example 3

[0045] A method for microbial identification based on ion mobility spectrometry, applicable to the microbial identification device based on ion mobility spectrometry described in Example 2, comprises the following steps:

[0046] S1. Sample ionization and ion collection: The laser component 140 vertically bombards the sample on the sample target plate 120 to generate ions. A high voltage electric field is briefly applied between the sample target plate 120 and the extraction electrode 160 to extract the ions. After the high voltage electric field is eliminated, the ion sampler 170 draws in the extracted ions by forming an airflow through the pressure difference between its two ends.

[0047] S2. Ion focusing and injection: Ion funnel 200 transmits and focuses the ions collected by ion sampler 170, and ion gate 300 controls the injection of focused ions into traveling wave migration separation zone 400 through periodic voltage switching.

[0048] S3. Ion separation: Traveling wave migration separation zone 400 separates ions based on the difference in their migration rate in the electric field.

[0049] S4. Generation of ion mobility spectrum: Detector 500 detects the separated ions, converts the ion signal into an electrical signal, and generates ion mobility spectrum.

[0050] S5. Identification and Analysis: The data analysis module performs similarity matching between ion mobility spectra and reference spectra in the spectral database, or uses machine learning models to perform pattern recognition on ion mobility spectra.

[0051] The above embodiments are only for illustrating the technical concept and features of this utility model. Their purpose is to enable those skilled in the art to understand the content of this utility model and implement it. They should not be used to limit the protection scope of this utility model. All equivalent changes or modifications made in accordance with the spirit and essence of this utility model should be covered within the protection scope of this utility model.

Claims

1. An ion source, characterized in that, The device includes a housing (110) and a sample target plate (120), an XY stage (130), a laser assembly (140), a camera assembly (150), an extraction electrode (160), and an ion sampler (170) disposed within the housing (110). The sample target plate (120) is mounted on the bottom plate of the housing (110) via the XY stage (130). The laser assembly (140) and the camera assembly (150) are disposed above the sample target plate (120). The laser assembly (140) is orthogonally arranged to the sample target plate (120). The extraction electrode (160) is disposed between the sample target plate (120) and the laser assembly (140). The ion sampler (170) is disposed between the sample target plate (120) and the extraction electrode (160), and is arranged parallel to the sample target plate (120). The interior of the housing (110) is an atmospheric pressure environment.

2. The ion source according to claim 1, characterized in that, It also includes an auxiliary gas line (180) and a heating component (190); the auxiliary gas line (180) is disposed between the sample target plate (120) and the extraction electrode (160), and the auxiliary gas line (180) is disposed opposite to the ion sampler (170), and the heating component (190) is mounted on the ion sampler (170).

3. The ion source according to claim 1, characterized in that, The laser assembly (140) includes a laser source (141), a laser collimating lens (142), and a laser focusing lens (143); the laser source (141), the laser collimating lens (142), and the laser focusing lens (143) are arranged sequentially from top to bottom, and the laser source (141) is mounted on the top plate of the housing (110).

4. The ion source according to claim 3, characterized in that, The camera assembly (150) includes a camera (151) and a camera lens (152); the camera (151) is mounted on the top plate of the housing (110) and is located on one side of the laser light source (141); the camera lens (152) is located between the sample target plate (120) and the camera (151).

5. The ion source according to claim 1, characterized in that, The ion sampler (170) is a capillary or a sampling cone.

6. A microbial identification device based on ion mobility spectrometry, characterized in that, The device includes an ion source, an ion funnel (200), an ion gate (300), a traveling wave migration separation region (400), a detector (500), and a data analysis module as described in any one of claims 1-5; the ion source is connected to the traveling wave migration separation region (400) through the ion funnel (200); the ion gate (300) is located at the outlet of the ion funnel (200) and is used to control the entry of ions into the traveling wave migration separation region (400); the detector (500) is installed on the side of the traveling wave migration separation region (400) away from the ion funnel (200), the detector (500) is communicatively connected to the data analysis module, and the ion funnel (200) is sealed in a chamber, the chamber being connected to a vacuum device.

7. The microbial identification device based on ion mobility spectrometry according to claim 6, characterized in that, The traveling wave migration separation region (400) includes an ion transport channel (410), the inner wall of which is attached to a flexible circuit board.

8. The microbial identification device based on ion mobility spectrometry according to claim 7, characterized in that, Multiple parallel electrodes are arranged on the flexible circuit board.

9. The microbial identification device based on ion mobility spectrometry according to claim 7 or 8, characterized in that, The cross-section of the ion transport channel (410) is circular or square.

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