Experimental device for stress treatment of grass seedlings

Abstract

The utility model relates to plant physiology experiment equipment technical field, concretely is a kind of grass plant seedling stage stress treatment experimental device, including transparent box, left and right two rows of circular planting hole, central scale baffle, light-avoiding cover box, drain hole and label slot. The transparent box is cuboid structure, and 6 circular planting holes are respectively arranged in left and right two rows;Every compartment back is equipped with the drain hole of 2cm diameter, and it is equipped with rotary red plastic plug to control drainage;The label slot of 4cm long 2cm wide is arranged on the outer wall of compartment front face, for inserting experimental label card;Box body central is equipped with vertical baffle, and vertical scale is etched on the both sides of baffle and is used to measure root length;Box body exterior is matched with black light-proof cover box, can completely cover device and realize light-avoiding.The utility model has the advantages of providing efficient, accurate integrated experimental tool for grass plant seedling stage stress research.

Description

Technical Field

[0001] This utility model relates to the technical field of plant physiological experimental equipment, specifically a device for treating stress in the seedling stage of herbaceous plants. More particularly, it relates to a device capable of simultaneously conducting experiments with stress treatment and a non-stress control, non-destructive dynamic observation of root growth, and efficient switching of the experimental environment. Root growth is directly observed through a transparent box and graduated partitions, avoiding experimental interference. Background Technology

[0002] Research on seedling stress in herbaceous plants is an important field of plant physiology, involving the simulation and analysis of various environmental factors such as drought and salinity. Current plant stress experimental devices mostly employ fixed compartment designs, such as patent CN204616641U, or rely on manual root length measurement, such as patent CN203981074U. These devices have significant drawbacks: First, traditional devices require frequent removal of seedlings to measure root growth, which can easily damage the roots and lead to data inaccuracies. Second, insufficient isolation between compartments allows liquids to easily seep through the partitions, causing cross-contamination and affecting experimental accuracy. Third, light-protected operations rely on external darkroom transfers, interrupting experimental continuity and reducing research efficiency. For example, although the seedling device disclosed in patent CN203981074U can achieve basic culture functions, it cannot achieve simultaneous experiments of stress treatment and non-stress control, and it lacks a design for non-destructive observation of root length; while the interconnected seedling tray described in patent CN2012200587093 can achieve nutrient balance through interconnected slots, but it leads to the extension of the root system, which can easily cause serious damage when lifting seedlings, and it cannot meet the needs of high-precision stress research in the seedling stage of grass plants.

[0003] Addressing the shortcomings of existing technologies, such as significant measurement interference, poor isolation, cumbersome light-shielding operations, and inconvenience in comparing treatment and control groups, this applicant has innovatively designed an experimental device that enables simultaneous experiments with stress treatments and non-stress controls, non-destructive dynamic observation of root growth, and isolated culture, taking into account the characteristics of experiments on herbaceous plants during the seedling stage. Through the synergistic effect of independent compartment design, a central graduated partition, and a light-shielding enclosure, it achieves independent control of different stress conditions, real-time observation and comparison of root growth, and efficient switching of experimental environments, providing a reliable tool for precise research on the stress response mechanisms of herbaceous plant seedlings. Utility Model Content

[0004] The purpose of this invention is to overcome the shortcomings of the existing technology and provide a device for stress treatment of herbaceous plants during the seedling stage. This device enables simultaneous experiments with stress treatment and no-stress control, non-destructive observation of root growth, and efficient switching of the experimental environment. It solves the problems of large measurement interference, poor isolation, and cumbersome operation in light-avoidance methods of traditional devices, and provides a precise and efficient integrated experimental tool for research on stress during the seedling stage of herbaceous plants.

[0005] This utility model is achieved through the following technical solution:

[0006] The device includes a transparent box, a fixing component, an integrated scale system, a label slot, and a light-proof sleeve. The transparent box is a cuboid structure made of transparent acrylic or polycarbonate, with a removable top cover. It is divided into two rows, each with six circular planting holes, 2cm in diameter, spaced 1.5cm apart. Each compartment has a 2cm diameter drainage hole on the back, fitted with a rotating red plastic plug, with the drainage channel opened and closed via a threaded connection. The front outer wall of each compartment has a 4cm x 2cm label slot, with a limiting protrusion at the bottom for inserting experimental labels. The fixing component is a circular sponge made of polyurethane foam, 2cm in diameter and 1cm thick, with a 0.5cm diameter through-hole in the center. The inner wall of the through-hole is coated with a hydrophobic coating to hold the seedling roots and prevent solution retention. The sponge is removable and reusable. The integrated scale system includes a 0.5cm thick vertical partition in the center of the box, with 0-15cm vertical graduations on both sides formed by laser etching. The graduation unit is millimeters, and the line spacing is 1mm. The graduation area is covered with a scratch-resistant polyester film protective layer, allowing direct observation of root length through the transparent box. The light-proof sleeve is made of black opaque material with a non-slip rubber pad on the bottom for quick switching between light and dark conditions. The inner wall of the circular planting hole has a frosted surface to enhance the friction between the sponge and the hole wall, preventing displacement during the experiment; the rotating red plastic stopper has a non-slip textured surface for easy operation.

[0007] The advantages of this utility model are:

[0008] 1. Stress-controlled independent control: The design of two independent compartments on the left and right sides enables simultaneous experiments under stress and without stress conditions, supporting simultaneous experiments with multiple variables;

[0009] 2. Dynamic observation system: Root growth can be directly observed through a transparent box and graduated partitions, enabling dynamic monitoring of the hydroponic device.

[0010] 3. Non-destructive observation: The integrated scale partition and transparent box allow for direct measurement of root length, avoiding damage to the root system caused by traditional manual measurement;

[0011] 4. Highly efficient isolation and light protection: Independent planting holes prevent cross-contamination of solutions, and the light-proof box allows for quick switching between light and dark environments, ensuring experimental continuity;

[0012] 5. Precise control and data management: The drainage hole design allows for flexible control of moisture conditions, and the label slot standardizes experimental labeling, improving data tracking efficiency;

[0013] 6. Low cost and repeatability: The sponge fixing components and detachable lid design simplify operation and reduce experimental costs.

[0014] This invention provides a high-precision, multi-functional integrated experimental tool for studying the stress response mechanism of herbaceous plants during the seedling stage, through the above-mentioned technical solution. It is suitable for quantitative analysis of various stress scenarios such as drought, salinity, and waterlogging.

[0015] Device Structure

[0016] 1. Transparent Box: Rectangular structure, made of transparent acrylic or polycarbonate. The lid is removable, and the top has two rows of circular planting holes, six in each row, with a hole diameter of 2cm and a spacing of 1.5cm. Each compartment has a 2cm diameter drainage hole on the back, equipped with a rotating red plastic plug.

[0017] 2. Fixing component: A circular sponge made of polyurethane foam material, 2cm in diameter and 1cm thick, with a 0.5cm diameter through hole in the center, and the inner wall is coated with a hydrophobic coating.

[0018] 3. Integrated scale system: The box has a vertical partition in the center, 0.5cm thick, and laser-etched vertical scales of 0-15cm on both sides, with the scale unit being millimeters and the line spacing being 1mm, covered with a scratch-resistant polyester film protective layer.

[0019] 4. Label slot: Each compartment has a 4cm long × 2cm wide groove on the front outer wall, with a limit protrusion at the bottom.

[0020] 5. Light-proof box: Black, opaque material with anti-slip rubber pads on the bottom.

[0021] Advantages of the device

[0022] 1. Stress-controlled independent control: The design of two independent compartments on the left and right sides enables simultaneous experiments under stress and without stress conditions, supporting simultaneous experiments with multiple variables;

[0023] 2. Non-destructive observation: The integrated graduated partition and transparent box allow for direct measurement of root length, avoiding damage to the root system caused by traditional manual measurement.

[0024] 3. Dynamic observation system: Root growth can be directly observed through a transparent box and graduated partitions, enabling dynamic monitoring of the hydroponic device.

[0025] 4. Highly efficient isolation and light protection: Independent planting holes prevent cross-permeability of solutions, and the light-proof box allows for quick switching between light and dark environments, ensuring the continuity of experiments.

[0026] 5. Precise control and data management: The drainage hole design allows for flexible control of moisture conditions, and the label slot standardizes experimental labeling, improving data tracking efficiency.

[0027] 6. Low cost and repeatability: The sponge fixing components and detachable lid design simplify operation and reduce experimental costs. Attached Figure Description

[0028] Appendix Figure 1 This is a schematic diagram of the structure of the hydroponic device of this utility model.

[0029] Appendix Figure 2 Right view of the device body

[0030] Appendix Figure 3 Right view of the device compartment

[0031] Appendix Figure 4 For the installation of light-shielding casing

[0032] Appendix Figure 5 Front view of the device

[0033] Appendix Figure 6 Top view of the device

[0034] Appendix Figure 7 Overall diagram of the device Detailed Implementation

[0035] Specific Implementation Method 1: This implementation method for treating stress in herbaceous plant seedlings includes a hydroponic box 1, drainage holes 1-1 on the back of the hydroponic box compartments, label grooves 1-2 on the front of the hydroponic box compartments, a partition 2, vertical graduations 2-1 on both sides of the partition (0-15cm), a hydroponic box lid 3, two rows of circular planting holes 3-1 on the top of the lid, and a light-shielding sleeve 4. In this implementation method, herbaceous plant seedlings are placed in the circular planting holes of the transparent box during the experiment, and the seedling roots are secured by a sponge fixing component. Different stress conditions, such as drought and salinity, are applied in different compartments according to experimental requirements. The drainage holes control the moisture conditions within the compartments, and the drainage channels are flexibly opened and closed using a rotating red plastic plug. During the experiment, root growth can be directly observed through the transparent box and integrated graduation system without frequently removing the seedlings, thus avoiding damage to the roots. Simultaneously, the light-shielding sleeve allows for quick switching between light and dark conditions to meet the needs of different experimental stages. The label groove is used to insert experimental label cards for convenient recording and tracking of experimental data. After the experiment, the sponge fixing component can be removed, cleaned, and reused, reducing the cost of the experiment.

[0036] Specific Implementation Method Two: This implementation method differs from Specific Implementation Method One in that a rotary red plastic plug drain valve is installed on the drain hole.

[0037] Specific Implementation Method Three: This implementation method differs from Specific Implementation Method One or Two in that a sponge is filled between the planting hole 3-1 and the hydroponic seedling. In this implementation method, the seedling is fixed in the planting hole by wrapping the stem near the root with the sponge.

Claims

1. An experimental apparatus for stress treatment of herbaceous plants during the seedling stage, characterized in that... include: Transparent box: Rectangular structure, made of transparent acrylic or polycarbonate, with a detachable lid. The top is divided into two rows, each with 6 circular planting holes, 2cm in diameter and 1.5cm apart. Each compartment has a 2cm diameter drainage hole on the back, with a rotating red plastic plug. Fixing components: Each circular planting hole contains a 2cm diameter, 1cm thick circular sponge made of polyurethane foam. It has a 0.5cm diameter through hole in the center to hold the seedling roots and fix them in the hole. The sponge can be removed and reused. Integrated scale system: A vertical partition with a thickness of 0.5cm is set in the center of the box, and vertical scales are etched on both sides, ranging from 0-15cm. The scale unit is millimeters, the scale line spacing is 1mm, and the scale area is covered with a transparent protective film. Label slot: Each compartment has a 4cm long x 2cm wide groove on the front outer wall for inserting experimental label cards; Light-proof box: Black opaque material, covering the transparent box body, with anti-slip rubber pads on the bottom; Dynamic observation system: Root growth can be directly observed through a transparent box and graduated partitions, enabling dynamic monitoring of the hydroponic device.

2. The apparatus according to claim 1, characterized in that: The inner wall of the circular implantation hole is frosted to enhance the friction between the sponge and the hole wall.

3. The apparatus according to claim 1, characterized in that: The rotating red plastic plug has anti-slip texture on its surface, and the plug body is threaded to match the drain hole, which can completely seal or open the drain channel.

4. The apparatus according to claim 1, characterized in that: The bottom of the label slot is provided with a limiting protrusion to prevent the label from slipping off.

5. The apparatus according to claim 1, characterized in that: The scales on both sides of the vertical partition are formed by laser etching, and the transparent protective film covering the scale area is a scratch-resistant polyester film.

6. The apparatus according to claim 1, characterized in that: The inner wall of the circular sponge through-hole is coated with a hydrophobic coating to prevent solution retention.

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