Multifunctional spin column

Abstract

The utility model discloses a multifunctional turnover chromatographic column relates to chromatographic column technical field, specifically a kind of multifunctional turnover chromatographic column, including support and chromatographic column body, column tail flange, column head flange, the bottom of the support is equipped with universal wheel, the top of the support is equipped with rotating assembly, rotating assembly is installed on rotating arm, hydraulic oil cylinder is installed on the rotating arm, the output end of the hydraulic oil cylinder is equipped with lifting arm, the end side surface of the lifting arm and the side surface of connecting barrel are all set with fixed pin hole, and fixed pin hole is inserted with fixed pin, the multifunctional turnover chromatographic column, by rotating assembly and lifting arm and rotating arm and the cooperation use of connecting barrel, fixed pin, and then it is convenient to disassemble and assemble column head flange and column tail flange, it is easy to operate, can reduce manual intervention in disassembly process, reduce the labor intensity of staff, make disassembly process more safe.

Description

Technical Field

[0001] This utility model relates to the field of chromatography column technology, specifically a multifunctional flip chromatography column. Background Technology

[0002] Industrial preparative chromatography columns are widely used separation and purification equipment, and are commonly used in biopharmaceutical, pharmaceutical chemistry, and food industries. They have advantages such as high efficiency, accuracy, controllability, and suitability for continuous production. They play a crucial role in the separation and purification process and are one of the core equipment for the separation and purification of high value-added products.

[0003] The separation principle of chromatography columns used in industrial production is based on the fact that the components in a mixture have different distribution coefficients in the stationary and mobile phases. Separation between components is achieved through adsorption, desorption, re-adsorption, and re-desorption processes. This method can efficiently separate and purify target substances, precisely control different separation conditions, and obtain target substances of varying purities, even high-purity substances. The entire operation is controllable and suitable for large-scale industrial production. However, as production scale gradually increases, the size of chromatography columns also becomes larger, making their use and disassembly increasingly inconvenient.

[0004] Secondly, conventional large-diameter chromatography columns used in industrial production have a simple structural design, and most are fixed to a support and cannot be moved or rotated. This makes loading, unloading, or cleaning the chromatography column cumbersome and complicated.

[0005] For example, Chinese Patent Publication No. CN213077562U discloses a chromatography column with a flipping mechanism. In this design, the frame on which the chromatography column is mounted is set on a base via a flipping mechanism. The driving component in the flipping mechanism adjusts the angle between the plane of the flipping base and the plane of the base, thereby changing the angle between the chromatography column mounted on the frame and the horizontal direction. This achieves the goal of tilting the chromatography column and performing packing and cleaning operations without manual intervention.

[0006] However, when using large-diameter industrial chromatography columns, the column head and tail flanges of this method are very heavy, weighing anywhere from tens of kilograms to hundreds of kilograms or even more. Therefore, disassembling and reassembling the column head and tail flanges is very difficult. Therefore, we propose a multifunctional flip chromatography column. Utility Model Content

[0007] To address the shortcomings of existing technologies, this invention provides a multifunctional flip chromatography column, which solves the problems mentioned in the background section.

[0008] To achieve the above objectives, this utility model provides the following technical solution: A multifunctional tilting chromatography column includes a support, a chromatography column body, a column tail flange, and a column head flange. The support has casters at its bottom and a rotating assembly at its top. A rotating arm is mounted on the rotating assembly, and a hydraulic cylinder is mounted on the rotating arm. A lifting arm is mounted at the output end of the hydraulic cylinder. Fixing pin holes are provided on the side of the lifting arm and the side of the connecting cylinder, and fixing pins are inserted into these holes. Rotating shafts are mounted on both sides of the support via bearings. The chromatography column body is fixedly mounted at one end of each rotating shaft. A column tail flange is bolted to the bottom of the chromatography column body, and the column tail flange has a liquid outlet. A column head flange is mounted to the top of the chromatography column body via quick-release bolts. Two connecting cylinders are fixedly mounted on both the column head flange and the column tail flange.

[0009] A tilting control box is fixedly installed on one side of the bracket. A drive source is provided outside the tilting control box. One end of one of the rotating shafts extends into the tilting control box and is equipped with a worm gear reducer, which is connected to the drive source.

[0010] Preferably, a mounting plate is fixedly installed on the top of the bracket, a rotating disk is rotatably installed on the mounting plate, the rotating disk is fixedly connected to the rotating arm, a drive motor is installed at the bottom of the mounting plate, and the output shaft of the drive motor passes through the mounting plate and is connected to the middle of the rotating disk. A positioning pin is inserted into the rotating disk, and a hole adapted to the end of the positioning pin is opened on the mounting plate.

[0011] Preferably, an installation cylinder is fixedly installed on the column head flange, the installation cylinder has a packing inlet on one side and an eluent outlet at the top of the installation cylinder.

[0012] Preferably, plugs are movably installed in both the packing inlet and the eluent outlet.

[0013] Preferably, the drive source is a rotary handwheel or a motor.

[0014] Preferably, the rotating arm is equipped with a hydraulic drive assembly and a control panel. The hydraulic drive assembly includes a hydraulic oil tank and components such as an oil pump installed inside the hydraulic oil tank, and the hydraulic drive assembly is connected to the hydraulic cylinder.

[0015] Preferably, a pressure gauge and an exhaust valve are installed on the column head flange.

[0016] This invention provides a multifunctional tilting chromatography column with the following advantages:

[0017] 1. This multifunctional tilting chromatography column, through the cooperation of rotating components, lifting arms, rotating arms, connecting cylinders, and fixing pins, facilitates the disassembly and assembly of the column head flange and column tail flange. It is easy to operate, reduces manual intervention during disassembly and assembly, lowers the labor intensity of workers, and makes the disassembly and assembly process safer.

[0018] 2. This multi-functional tilting chromatography column can be tilted by operating the tilting handwheel or by using a motor, which facilitates the cleaning and replacement of the column head flange, column tail flange, sieve plate, and chromatography column, making operation more convenient. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the structure of this utility model;

[0020] Figure 2 This is a schematic diagram of the front structure of this utility model;

[0021] Figure 3 This is a schematic diagram of the structure of the present invention from the left view;

[0022] Figure 4 This is a top view of the structure of this utility model.

[0023] In the diagram: 1. Support; 2. Casters; 3. Outlet; 4. Column tail flange; 5. Chromatography column; 6. Quick-release bolt; 7. Control panel; 8. Hydraulic oil tank; 9. Hydraulic cylinder; 10. Lifting arm; 11. Eluent inlet; 12. Packing inlet; 13. Rotating shaft; 14. Pressure gauge; 15. Column head flange; 16. Fixing pin; 17. Rotating arm; 18. Exhaust valve; 19. Connecting cylinder; 20. Tilting handwheel; 21. Tilting control box; 22. Rotating disc; 23. Positioning pin; 24. Mounting disc; 25. Mounting cylinder. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0025] Example 1: Refer to Figures 1-4 This utility model provides a multifunctional flip-type chromatography column, including a support 1, a chromatography column body 5, a column tail flange 4, and a column head flange 15. The bottom of the support 1 is equipped with casters 2, and the top of the support 1 is equipped with a rotating assembly. A rotating arm 17 is mounted on the rotating assembly, and a hydraulic cylinder 9 is mounted on the rotating arm 17 by screws. A lifting arm 10 is fixedly mounted on the output end of the hydraulic cylinder 9.

[0026] The support 1 has a rotating shaft 13 mounted on both sides via bearings. A chromatography column 5 is fixedly mounted on one end of the rotating shaft 13. A column tail flange 4 is bolted to the bottom of the chromatography column 5. The column tail flange 4 has an outlet 3. A column head flange 15 is mounted on the top of the chromatography column 5 via quick-release bolts 6. Two connecting cylinders 19 are fixedly mounted on both the column head flange 15 and the column tail flange 4.

[0027] The lifting arm 10 and the connecting cylinder 19 are provided with fixing pin holes, and fixing pins 16 are inserted into the fixing pin holes.

[0028] A tilt control box 21 is fixedly installed on one side of the bracket 1. A drive source is provided outside the tilt control box 21. One end of a rotating shaft 13 extends into the tilt control box 21 and is equipped with a worm gear reducer. The worm gear reducer is connected to the drive source and has a locking function to prevent the rotating shaft 13 from rotating actively.

[0029] A mounting plate 24 is fixedly installed on the top of the bracket 1. A rotating plate 22 is rotatably mounted on the mounting plate 24. The rotating plate 22 is fixedly connected to the rotating arm 17. A drive motor is installed at the bottom of the mounting plate 24, and the output shaft of the drive motor passes through the mounting plate 24 and is connected to the middle of the rotating plate 22. A positioning pin 23 is inserted into the rotating plate 22. A hole adapted to the end of the positioning pin 23 is opened on the mounting plate 24. After rotating the rotating plate 22, the positioning pin 23 can be inserted into the hole on the mounting plate 24, thereby preventing the rotating arm 17 from shifting during the installation process.

[0030] An installation cylinder 25 is fixedly installed on the column head flange 15. A packing inlet 12 is provided on one side of the installation cylinder 25, and an eluent outlet 11 is provided at the top of the installation cylinder 25.

[0031] Both the packing inlet 12 and the eluent outlet 11 are equipped with plugs that are used to seal the packing inlet 12 and the eluent outlet 11.

[0032] The drive source is either the reversing handwheel 20 or a motor. When the drive source is the reversing handwheel 20, the shaft in the middle of the reversing handwheel 20 is connected to the worm gear reducer to achieve drive. When the drive source is a motor, the motor is connected to the reversing control box 21, and the motor output shaft is connected to the worm gear reducer, and the rotating shaft column 13 is driven to rotate by the worm gear reducer.

[0033] The rotating arm 17 is equipped with a hydraulic drive assembly and a control panel 7. The hydraulic drive assembly includes a hydraulic oil tank 8 and components such as an oil pump installed in the hydraulic oil tank 8. The hydraulic drive assembly is connected to the hydraulic cylinder 9. The control panel 7 has a built-in PLC controller and is electrically connected to the motor, drive motor, and hydraulic drive assembly.

[0034] A pressure gauge 14 and an exhaust valve 18 are installed on the column head flange 15.

[0035] Example 2, based on Example 1, includes a pressure transmitter installed on the column tail flange 4, and pneumatic ball valves installed in the exhaust valve, packing inlet 12, eluent port 11, and outlet 3 respectively. The pneumatic ball valves are connected to the pressure transmitter, which detects the pressure and controls the pneumatic ball valves. The pressure transmitter is an integrated device with detection and control functions.

[0036] In summary, when loading the chromatography packing material into this multifunctional tilting chromatography column, the column must first be properly installed. First, install the column tail flange 4 onto the tail end of the chromatography column 5 and secure it with bolts. Then, install the column head flange 15 onto the head of the chromatography column 5 and secure it with quick-release bolts 6. Next, pump the homogenized packing material slurry into the column using a pneumatic diaphragm pump and piping from the packing inlet 12. The homogenate continuously flows from the outlet 3 on the column tail flange 4 into the solvent storage tank located below the column tail flange 4. After the packing material slurry has been completely pumped into the chromatography column 5, seal the packing inlet 12 with a plug. Remove the plug at the eluent outlet 11 and connect either the sample loading pump or the elution solvent pump to pump in the mixture solution to be separated for sample loading or elution purification.

[0037] If the separation and purification effect deteriorates, the packing material should be replaced after the elution and purification operation is completed. First, ensure that the outlet 3 on the column tail flange 4 is unobstructed and open the vent valve 18 on the column head flange 15. Observe that the pressure gauge 14 on the column head flange 15 is within the safe operating pressure range. Loosen the quick-release bolts 6 on the column head flange 15, align the fixing pin hole at the end of the lifting arm 10 with the fixing pin hole on the connecting cylinder 19 on the column head flange 15, insert the fixing pin 16, and start the hydraulic cylinder 9 through the hydraulic drive component using the corresponding control button on the control panel 7. This will lift the column head flange 15 upwards, separating it from the chromatography column 5. Start the drive motor to drive the rotating disk 22 to rotate, which in turn drives the rotating arm 17 to rotate.

[0038] After rotating the column head flange 15 by a certain angle using the rotating arm 17, it can be placed on the lifting platform or transferred to the storage rack for easy disassembly and thorough cleaning. The tilting control box 21 contains a worm gear reducer.

[0039] Operate the flip handwheel 20. The shaft of the flip handwheel 20 is connected to the worm gear reducer, which in turn rotates the rotating shaft column 13 to rotate, slowly flipping the chromatography column 5. If a motor drive is used, the output shaft of the motor drives the rotating shaft column 13 to rotate through the worm gear reducer. The motor is fixed to the flip control box 21, which also achieves the flipping of the chromatography column 5.

[0040] Pour the packing material into the packing container to thoroughly clean the chromatography column 5 and the column tail flange 4. If it is necessary to clean or replace the sieve plate in the column tail flange 4 separately, continue to rotate the chromatography column 5 until the bottom surface of the column tail flange 4 faces upward. After loosening all bolts, use the rotating arm 17 and the lifting arm 10 to remove the column tail flange 4, and then remove the sieve plate for deep cleaning or replacement.

[0041] After the sieve plate is processed, repeat the above steps in reverse order. Install the column tail flange 4, then slowly rotate it 180°. Install the column head flange 15 using the rotating arm 17 and lifting arm 10. Install the quick-release bolts 6, and then proceed with subsequent operations. The column head flange 15 and column tail flange 4 have the same structure and are installed in mirror image. By rotating the chromatography column, it can be used in both directions equally.

[0042] Finally, the following points should be noted: First, in the description of this application, it should be noted that, unless otherwise specified and limited, the terms "installation", "connection", and "linkage" should be interpreted broadly, and can be mechanical or electrical connections, or internal connections between two components, or direct connections. "Up", "down", "left", "right", etc. are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may change.

[0043] Secondly: The accompanying drawings of the embodiments disclosed in this utility model only involve the structures involved in the embodiments disclosed in this utility model. Other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of this utility model can be combined with each other.

[0044] Finally: The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A multifunctional inverted chromatography column, comprising a support (1), a chromatography column body (5), a column tail flange (4), and a column head flange (15), characterized in that: The bottom of the bracket (1) is equipped with casters (2), the top of the bracket (1) is equipped with a rotating assembly, a rotating arm (17) is mounted on the rotating assembly, a hydraulic cylinder (9) is mounted on the rotating arm (17), a lifting arm (10) is mounted on the output end of the hydraulic cylinder (9), a fixing pin hole is provided on the side of the end of the lifting arm (10) and the side of the connecting cylinder (19), and a fixing pin (16) is inserted into the fixing pin hole. The two sides of the bracket (1) are equipped with rotating shafts (13) through bearings. A chromatography column (5) is fixedly mounted on one end of the rotating shaft (13). A column tail flange (4) is bolted to the bottom of the chromatography column (5). A liquid outlet (3) is provided on the column tail flange (4). A column head flange (15) is mounted on the top of the chromatography column (5) through quick-release bolts (6). Two connecting cylinders (19) are fixedly mounted on both the column head flange (15) and the column tail flange (4). A tilting control box (21) is fixedly installed on one side of the bracket (1). A drive source is provided outside the tilting control box (21). One end of a rotating shaft (13) extends into the tilting control box (21) and is equipped with a worm gear reducer. The worm gear reducer is connected to the drive source.

2. The multifunctional tilting chromatography column according to claim 1, characterized in that: A mounting plate (24) is fixedly installed on the top of the bracket (1). A rotating plate (22) is rotatably installed on the mounting plate (24). The rotating plate (22) is fixedly connected to the rotating arm (17). A drive motor is installed at the bottom of the mounting plate (24). The output shaft of the drive motor passes through the mounting plate (24) and is connected to the middle of the rotating plate (22). A positioning pin (23) is inserted into the rotating plate (22). A hole adapted to the end of the positioning pin (23) is opened on the mounting plate (24).

3. The multifunctional tilting chromatography column according to claim 1, characterized in that: An installation cylinder (25) is fixedly installed on the column head flange (15). A packing inlet (12) is provided on one side of the installation cylinder (25), and an eluent port (11) is provided at the top of the installation cylinder (25).

4. A multifunctional tilting chromatography column according to claim 1, characterized in that: Both the packing inlet (12) and the eluent outlet (11) are equipped with movable plugs.

5. A multifunctional tilting chromatography column according to claim 1, characterized in that: The drive source is a rotary handwheel (20) or a motor.

6. A multifunctional flip chromatography column according to claim 1, characterized in that: A control panel (7) is mounted on the rotating arm (17).

7. A multifunctional tilting chromatography column according to claim 1, characterized in that: A pressure gauge (14) and an exhaust valve (18) are installed on the column head flange (15).

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