A perinatal hematopoietic stem cell perfusion collection device that is easy to disassemble

Abstract

The utility model discloses a kind of perinatal hematopoietic stem cell's perfusion collection devices of easy disassembly, it is related to medical equipment technical field, including base and install on the upper surface of base crushing chamber, the outer wall of the crushing chamber is equipped with interface, the inside thread of the interface is connected with threaded tube, collecting tube is fixedly installed in the threaded tube end, one-way valve is installed on the threaded tube, the inside connection of the crushing chamber has the negative pressure component of making the negative pressure in crushing chamber and self-cleaning component, the bottom of the crushing chamber is connected with filtering component by connecting pipe, centrifugal separation component is connected in the lower end of the filtering component, water pump can pump into the inside of water pipe and annular shower head with disinfectant, atomization is sprayed to the inner wall of crushing chamber by annular shower head, flushing disinfection is carried out to the inside of crushing chamber, simultaneously, by blade rotation with driving shaft, blade and disinfectant can be driven to generate vortex, so that flushing the inner wall of crushing chamber and blade multiple times, improve cleaning and disinfection effect.

Description

Technical Field

[0001] This utility model relates to the field of medical devices, and in particular to a perfusion collection device for perinatal hematopoietic stem cells that is easy to disassemble. Background Technology

[0002] Perinatal tissues mainly refer to the placenta, umbilical cord, and the blood they contain. The placenta is the organ through which the fetus and mother exchange substances; it is a transitional organ formed by the union of the embryonic membranes and the mother's endometrium. Perinatal stem cells refer to stem cells derived from the perinatal tissues of newborns. They can be directly extracted from perinatal tissues such as the placenta or umbilical cord, making the collection convenient, harmless to both mother and fetus, and with low contamination levels. Furthermore, they possess a stronger ability to regenerate various tissues and organs and enhance the body's potential, making them more suitable for clinical treatment. Perinatal hematopoietic stem cell collection equipment is a medical device used to collect hematopoietic stem cells from perinatal tissues (such as the placenta and umbilical cord).

[0003] In the prior art, a collection device for placental hematopoietic stem cells, disclosed in CN211445690U, includes a housing. Inside the housing is a placental tissue pulverizing chamber, which is sequentially connected to a placental tissue filtering chamber, a centrifuge, and a placental hematopoietic stem cell cryopreservation chamber. A drive shaft is located within the placental tissue pulverizing chamber, and multiple cutting blades are mounted on the drive shaft. The ends of the cutting blades are fitted with brushes that abut against the sidewall of the placental tissue pulverizing chamber. A stirring paddle is also located at one end of the drive shaft, and the stirring paddle is also fitted with multiple brushes that abut against the bottom of the placental tissue pulverizing chamber. A filter screen is located within the placental tissue filtering chamber. The end of the filter screen is slidably connected to a groove on the inner wall of the placental tissue filtering chamber via a slider. An elliptical turntable is located below the filter screen. This collection device effectively solves the problem of contamination during hematopoietic stem cell collection and has high collection efficiency.

[0004] In the above technical solutions, hematopoietic stem cells must be collected from placental tissue through crushing, enzymatic hydrolysis, filtration separation, and centrifugation. However, after collection, the residue inside the device cannot be automatically cleaned. The device needs to be disassembled and the debris inside the device needs to be cleaned. This method is difficult to clean and slow, which may affect the next collection of hematopoietic stem cells. Therefore, we propose a perinatal hematopoietic stem cell perfusion collection device that is easy to disassemble. Utility Model Content

[0005] To address the shortcomings of existing technologies, this invention provides a perinatal hematopoietic stem cell perfusion collection device that is easy to disassemble, thus solving the problems mentioned in the background section.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a perinatal hematopoietic stem cell perfusion collection device that is easy to disassemble, comprising a base and a grinding chamber mounted on the upper surface of the base. The outer wall of the grinding chamber has an interface, and a threaded tube is threadedly connected to the interface. A collection tube is fixedly installed at the end of the threaded tube, and a one-way valve is installed on the threaded tube. A negative pressure component and a self-cleaning component are connected inside the grinding chamber to generate negative pressure inside the grinding chamber. A filter component is connected to the bottom of the grinding chamber through a connecting pipe, and a centrifugal separation component is connected to the lower end of the filter component.

[0007] Preferably, a drive shaft is rotatably mounted inside the grinding chamber, and several blades are detachably mounted on the outer wall of the drive shaft. A grinding motor connected to the drive shaft is mounted at the bottom of the grinding chamber.

[0008] Preferably, the negative pressure assembly includes a negative pressure pump installed outside the base, the negative pressure pump being connected to the grinding chamber via a negative pressure pipe, and a negative pressure valve being installed on the negative pressure pipe.

[0009] Preferably, the self-cleaning component includes an annular nozzle fixedly installed on the top of the pulverizing chamber and a disinfection water tank disposed inside the base. A water pump is installed inside the disinfection water tank, and the output end of the water pump is connected to the annular nozzle through a water pipe.

[0010] Preferably, the filter assembly includes a filter box, inside which a conical filter element is installed. The end of the connecting pipe is located on the upper side of the conical filter element, and a discharge valve is installed inside the connecting pipe. Several drain pipes are connected to the inner wall of the filter box at the lower end of the conical filter element. An annular seal for sealing the drain pipes is slidably installed on the inner wall of the filter box, and an electric push rod for driving the annular seal to move up and down is installed on the top of the filter box.

[0011] Preferably, a funnel collecting end is installed inside the filter box on the lower side of the conical filter core, and the lower end of the funnel collecting end extends movably into the centrifugal separation component.

[0012] Preferably, the centrifugal separation assembly includes a centrifuge chamber installed at the bottom of the filter box, a hollow shaft rotatably mounted inside the centrifuge chamber, the upper end of the hollow shaft being rotatably sleeved on the outside of the lower end of the funnel collecting end, and the lower end of the hollow shaft rotatably extending to the outside of the centrifuge chamber and connected to a collecting box.

[0013] Preferably, the hollow shaft has a ring array of centrifuge tubes on its outer side, and the upper and lower ends of each centrifuge tube are connected to the interior of the hollow shaft. Solenoid valves are installed on both the upper and lower sides of the hollow shaft and the centrifuge tubes.

[0014] Preferably, a first bevel tooth is installed on the lower outer wall of the hollow shaft, a second bevel tooth is engaged with one side of the first bevel tooth, and a centrifugal motor for driving the second bevel tooth is installed on the lower inner and outer walls of the centrifuge.

[0015] This invention provides a perinatal hematopoietic stem cell perfusion collection device that is easy to disassemble, and has the following advantages compared with the prior art:

[0016] This design is an easy-to-disassemble perfusion collection device for perinatal hematopoietic stem cells. The water pump can pump disinfectant into the water pipe and the interior of the annular nozzle. The disinfectant is sprayed onto the inner wall of the crushing chamber through the annular nozzle to rinse and disinfect the interior of the crushing chamber. At the same time, the blade rotates with the drive shaft, which can drive the blade and the disinfectant to generate a vortex, thereby rinsing the inner wall of the crushing chamber and the blade multiple times, improving the cleaning and disinfection effect.

[0017] The electric push rod drives the annular seal to move upward, causing the annular seal to separate from the opening of the drain pipe. At this time, disinfectant water flows through the connecting pipe to the surface of the conical filter element. The disinfectant water washes the filtered material on the surface of the conical filter element to the outer periphery of the conical filter element and flows into the drain pipe. This can discharge the impurities generated by filtration from the equipment, complete the cleaning and disinfection of the filter components, and reduce the difficulty of cleaning and disinfecting the inside of the equipment. Attached Figure Description

[0018] Figure 1 A schematic diagram of the overall structure of a perinatal hematopoietic stem cell perfusion collection device that is easy to disassemble;

[0019] Figure 2 for Figure 1 Enlarged structural diagram at point a;

[0020] Figure 3 A schematic diagram of the internal structure of the pulverizing chamber of a perinatal hematopoietic stem cell perfusion collection device that is easy to disassemble;

[0021] Figure 4 for Figure 3 Enlarged structural diagram at point b

[0022] Figure 5 This is a schematic diagram of the internal structure of the drive shaft of a perinatal hematopoietic stem cell perfusion collection device that is easy to disassemble.

[0023] In the diagram: 1. Base; 2. Grinding chamber; 21. Drive shaft; 22. Blade; 23. Grinding motor; 3. Interface; 31. Threaded pipe; 32. One-way valve; 33. Collection pipe; 4. Negative pressure assembly; 41. Negative pressure pump; 42. Negative pressure pipe; 43. Negative pressure valve; 5. Self-cleaning assembly; 51. Annular nozzle; 52. Water pipe; 53. Water pump; 54. Disinfection pool; 6. Connecting pipe; 61. Discharge valve; 7. Filter assembly; 71. Filter box; 72. Conical filter element; 73. Sewage pipe; 74. Annular seal; 75. Electric push rod; 76. Funnel collection end; 8. Centrifugal separation assembly; 81. Centrifuge box; 82. Hollow shaft; 83. Centrifuge tube; 84. Solenoid valve; 85. First bevel gear; 86. Second bevel gear; 87. Centrifugal motor. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0025] Please see Figure 1-5 This invention provides a perinatal hematopoietic stem cell perfusion collection device that is easy to disassemble, including a base 1 and a crushing chamber 2 installed on the upper surface of the base 1. The outer wall of the crushing chamber 2 has an interface 3, and a threaded tube 31 is threadedly connected to the interface 3. A collection tube 33 is fixedly installed at the end of the threaded tube 31, and a one-way valve 32 is installed on the threaded tube 31. A negative pressure component 4 and a self-cleaning component 5 are connected inside the crushing chamber 2 to generate negative pressure. A filter component 7 is connected to the bottom of the crushing chamber 2 via a connecting pipe 6, and a centrifugal separation component 8 is connected to the lower end of the filter component 7. During use, the negative pressure component 4 generates negative pressure inside the crushing chamber 2, causing the threaded tube 31 connected to the crushing chamber 2 and the collection tube 33 to be threadedly connected to the collection tube 31 to be threadedly connected to the collection tube 3 ... The manifold 33 generates negative pressure, which allows placental tissue and enzymatic hydrolysate to be collected into the grinding chamber 2 through negative pressure suction. Then, the threaded tube 31 can be closed by the one-way valve 32. Next, the grinding motor 23 drives the drive shaft 21 and the blade 22 to rotate and cut the placental tissue. After grinding, the connecting tube 6 can be opened by the discharge valve 61, so that the inclined connecting tube 6 guides the ground material into the filter assembly 7 (the higher end of the connecting tube 6 is connected to the bottom of the grinding chamber 2, and the lower end of the connecting tube 6 is connected to the filter assembly 7). The filter assembly 7 filters the impurities of the ground material and guides the filtered solution into the centrifugal separation assembly 8 to quickly separate the hematopoietic stem cells in the solution.

[0026] The threaded tube 31 is installed inside the interface 3 by means of threads, which allows for quick disassembly and assembly of the threaded tube 31 and the collection tube 33 by rotation. This enables the collection tube 33 to be quickly disassembled and replaced after collection, improving the convenience of use and disassembly speed, and facilitating the cleaning, disinfection or replacement of the threaded tube 31 and the collection tube 33 after use.

[0027] like Figure 5 As shown, in this embodiment, a drive shaft 21 is rotatably mounted inside the crushing chamber 2, and several blades 22 are detachably mounted on the outer wall of the drive shaft 21. A crushing motor 23 connected to the drive shaft 21 is installed at the bottom of the crushing chamber 2. In use, the crushing motor 23 can drive the drive shaft 21 and the blades 22 to rotate, so that the blades 22 cut the placental tissue inside the crushing chamber 2.

[0028] like Figure 3 As shown, in this embodiment, the negative pressure component 4 includes a negative pressure pump 41 installed outside the base 1. The negative pressure pump 41 is connected to the grinding chamber 2 through a negative pressure pipe 42. A negative pressure valve 43 is installed on the negative pressure pipe 42. In use, the negative pressure pump 41 causes the negative pressure pipe 42 and the inside of the grinding chamber 2 to generate a negative pressure state.

[0029] like Figure 3 As shown, in this embodiment, the self-cleaning component 5 includes an annular nozzle 51 fixedly installed on the top of the grinding chamber 2 and a disinfection water tank 54 disposed inside the base 1. A water pump 53 is installed inside the disinfection water tank 54, and the output end of the water pump 53 is connected to the annular nozzle 51 through a water pipe 52. The disinfection water tank 54 is filled with disinfectant water. The water pump 53 can pump the disinfectant water into the water pipe 52 and the annular nozzle 51. The annular nozzle 51 atomizes and sprays it onto the inner wall of the grinding chamber 2 to rinse and disinfect the inside of the grinding chamber 2. At the same time, the blade 22 rotates with the drive shaft 21, which can drive the blade 22 and the disinfectant water to generate a vortex, thereby rinsing the inner wall of the grinding chamber 2 and the blade 22 multiple times, improving the cleaning and disinfection effect. After the grinding chamber 2 is cleaned, the connecting pipe 6 is opened through the discharge valve 61 to allow the disinfectant water to flow into the filter box 71.

[0030] like Figure 3 and Figure 4As shown, in this embodiment, the filter assembly 7 includes a filter box 71, a conical filter element 72 installed inside the filter box 71, the end of the connecting pipe 6 is located on the upper side of the conical filter element 72, a discharge valve 61 is installed inside the connecting pipe 6, a plurality of drain pipes 73 are connected to the inner wall of the filter box 71 at the lower end of the conical filter element 72, an annular seal 74 for sealing the drain pipes 73 is slidably installed on the inner wall of the filter box 71, an electric push rod 75 for driving the annular seal 74 to move up and down is installed on the top of the filter box 71, a funnel collecting end 76 is installed inside the filter box 71 at the lower side of the conical filter element 72, the lower end of the funnel collecting end 76 extends movably into the centrifugal separation assembly 8, during filtration, the pulverized material flows to the surface of the conical filter element 72 through the end of the connecting pipe 6, the conical filter element 72 filters the impurities inside the pulverized material, and the filtered solution flows into the funnel collecting end 76 and into the hollow shaft 82 through the lower end of the funnel collecting end 76.

[0031] During cleaning, the electric push rod 75 drives the annular seal 74 to move upward, causing the annular seal 74 to separate from the opening of the drain pipe 73. At this time, disinfectant water flows through the connecting pipe 6 to the surface of the conical filter element 72. The disinfectant water washes the filtered material on the surface of the conical filter element 72 to the outer periphery of the conical filter element 72 and flows into the drain pipe 73. This can discharge the impurities generated by filtration from the equipment, complete the cleaning and disinfection of the filter component 7, and reduce the difficulty of cleaning and disinfecting the inside of the equipment.

[0032] like Figure 3 and Figure 4As shown, in this embodiment, the centrifugal separation assembly 8 includes a centrifuge chamber 81 installed at the bottom of the filter box 71. A hollow shaft 82 is rotatably mounted inside the centrifuge chamber 81. The upper end of the hollow shaft 82 is rotatably sleeved on the outside of the lower end of the funnel collection end 76. The lower end of the hollow shaft 82 extends rotatably to the outside of the centrifuge chamber 81 and is connected to the collection box. Several centrifuge tubes 83 are arranged in a ring array outside the hollow shaft 82. The upper and lower ends of each centrifuge tube 83 are connected to the inside of the hollow shaft 82. Solenoid valves 84 are installed on both the upper and lower sides of the hollow shaft 82 and the centrifuge tubes 83. A first bevel tooth 85 is installed on the lower outer wall of the hollow shaft 82. A second bevel tooth 86 is meshed with one side of the first bevel tooth 85. A centrifugal motor 87 for driving the second bevel tooth 86 is installed on the inner and outer walls of the lower end of the centrifuge chamber 81. During separation, the solution flows through the lower end of the funnel collection end 76. The solution enters the hollow shaft 82 and several centrifuge tubes 83. The lower outer wall of the funnel collecting end 76 is rotatably connected to the upper inner wall of the hollow shaft 82. This allows the solution to flow and transfer within the sealed space, effectively protecting the solution and preventing infection. After the solution has completely entered the hollow shaft 82 and centrifuge tubes 83, the solenoid valve 84 located on the upper side of the centrifuge tubes 83 closes, isolating the hollow shaft 82, centrifuge tubes 83 from the funnel collecting end 76. At this time, the centrifuge motor 87 is started, which drives the second umbrella tooth 86 to drive the first umbrella tooth 85 and the hollow shaft 82 to rotate. The rotation of the hollow shaft 82 and centrifuge tubes 83 generates centrifugal force to separate hematopoietic stem cells. After separation, the solenoid valve 84 located at the bottom opens the bottom opening of the hollow shaft 82, thereby guiding the separated hematopoietic stem cells into the storage tank, completing the separation process.

[0033] During cleaning, disinfectant water flows into the hollow shaft 82 and several centrifuge tubes 83 through the lower end of the funnel collection end 76, thereby cleaning the interior of the hollow shaft 82 and several centrifuge tubes 83 and cleaning and disinfecting the centrifugal separation component 8, reducing the difficulty of cleaning and disinfection.

[0034] The working principle of this utility model is as follows: During use, the negative pressure component 4 generates negative pressure inside the grinding chamber 2, which in turn generates negative pressure in the threaded pipe 31 and the collection pipe 33 connected to the grinding chamber 2. The placental tissue and enzymatic hydrolysate can be collected into the grinding chamber 2 by the negative pressure suction. Then, the threaded pipe 31 can be closed by the one-way valve 32. Then, the grinding motor 23 drives the drive shaft 21 and the blade 22 to rotate and cut the placental tissue. After grinding, the connecting pipe 6 can be opened by the discharge valve 61, so that the inclined connecting pipe 6 guides the ground material into the filter component 7 (the higher end of the connecting pipe 6 is connected to the bottom of the grinding chamber 2, and the lower end of the connecting pipe 6 is connected to the filter component 7). The filter component 7 filters the impurities of the ground material and guides the filtered solution into the centrifugal separation component 8 to quickly separate the hematopoietic stem cells in the solution.

[0035] The above description is merely a preferred embodiment of this utility model. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of this utility model, and these improvements and modifications should also be considered within the scope of protection of this utility model. Structures, devices, and operating methods not specifically described or explained in this utility model are implemented according to conventional methods in the art, unless otherwise specified or limited.

Claims

1. A perfusion collection device for perinatal hematopoietic stem cells that is easy to disassemble, characterized in that, The device includes a base (1) and a grinding chamber (2) mounted on the upper surface of the base (1). The outer wall of the grinding chamber (2) is provided with an interface (3). The interface (3) is internally threaded with a threaded pipe (31). A collection pipe (33) is fixedly installed at the end of the threaded pipe (31). A one-way valve (32) is installed on the threaded pipe (31). The grinding chamber (2) is internally connected with a negative pressure component (4) to generate negative pressure in the grinding chamber (2) and a self-cleaning component (5). The bottom of the grinding chamber (2) is connected to a filter component (7) through a connecting pipe (6). The lower end of the filter component (7) is connected to a centrifugal separation component (8).

2. The perinatal hematopoietic stem cell perfusion collection device that is easy to disassemble according to claim 1, characterized in that, The crushing chamber (2) is rotatably mounted with a drive shaft (21), and a number of blades (22) are detachably mounted on the outer wall of the drive shaft (21). A crushing motor (23) connected to the drive shaft (21) is installed at the bottom of the crushing chamber (2).

3. The perinatal hematopoietic stem cell perfusion collection device that is easy to disassemble according to claim 1, characterized in that, The negative pressure assembly (4) includes a negative pressure pump (41) installed outside the base (1). The negative pressure pump (41) is connected to the crushing chamber (2) through a negative pressure pipe (42). A negative pressure valve (43) is installed on the negative pressure pipe (42).

4. The perinatal hematopoietic stem cell perfusion collection device that is easy to disassemble according to claim 1, characterized in that, The self-cleaning component (5) includes an annular nozzle (51) fixedly installed on the top of the grinding chamber (2) and a disinfection pool (54) set inside the base (1). A water pump (53) is installed inside the disinfection pool (54), and the output end of the water pump (53) is connected to the annular nozzle (51) through a water pipe (52).

5. The perinatal hematopoietic stem cell perfusion collection device that is easy to disassemble according to claim 1, characterized in that, The filter assembly (7) includes a filter box (71), inside which a conical filter element (72) is installed. The end of the connecting pipe (6) is located on the upper side of the conical filter element (72). A discharge valve (61) is installed inside the connecting pipe (6). Several drain pipes (73) are connected to the lower part of the inner wall of the filter box (71) at the conical filter element (72). An annular seal (74) for sealing the drain pipes (73) is slidably installed on the inner wall of the filter box (71). An electric push rod (75) for driving the annular seal (74) to move up and down is installed on the top of the filter box (71).

6. The perinatal hematopoietic stem cell perfusion collection device that is easy to disassemble according to claim 5, characterized in that, Inside the filter box (71), a funnel collection end (76) is installed on the lower side of the conical filter element (72), and the lower end of the funnel collection end (76) extends into the centrifugal separation component (8).

7. The perinatal hematopoietic stem cell perfusion collection device that is easy to disassemble according to claim 6, characterized in that, The centrifugal separation assembly (8) includes a centrifuge box (81) installed at the bottom of the filter box (71). A hollow shaft (82) is rotatably installed inside the centrifuge box (81). The upper end of the hollow shaft (82) is rotatably sleeved on the lower end of the funnel collection end (76). The lower end of the hollow shaft (82) extends rotatably to the outside of the centrifuge box (81) and is connected to a collection box.

8. The perinatal hematopoietic stem cell perfusion collection device that is easy to disassemble according to claim 7, characterized in that, The hollow shaft (82) has a ring array of centrifuge tubes (83) on its outside. The upper and lower ends of each centrifuge tube (83) are connected to the inside of the hollow shaft (82). Solenoid valves (84) are installed on both the upper and lower sides of the hollow shaft (82) and the centrifuge tubes (83).

9. The perinatal hematopoietic stem cell perfusion collection device that is easy to disassemble according to claim 8, characterized in that, A first bevel tooth (85) is installed on the lower outer wall of the hollow shaft (82), and a second bevel tooth (86) is meshed with one side of the first bevel tooth (85). A centrifugal motor (87) for driving the second bevel tooth (86) is installed on the lower inner and outer walls of the centrifuge box (81).

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