A cell well plate

Abstract

The utility model provides a kind of cell well plate, from bottom to top sequentially include substrate, middle layer, hydrophobic layer, the middle layer is equipped with a plurality of sample wells, the sample well is communicated with the substrate, electrode is provided on the substrate, and the electrode is located the sample well below;The electrode is used to generate uneven electric field, so that cell or bacteria is inclined to the side movement of uneven electric field and is settled in the bottom of the sample well;The middle layer is used to clean sample liquid;The hydrophobic layer is used to keep sample in the sample well and sample well between not connected and flows in series.The utility model adds electric field below well plate, improves cell settling velocity by dielectrophoresis force effect, and cell (or bacteria) is not easy to lose under the action of dielectrophoresis force after settling, and has better retention rate in laminar flow cleaning process.

Description

Technical Field

[0001] This utility model relates to the field of cell or virus culture devices, and in particular to a cell well plate. Background Technology

[0002] Existing well plates rely on gravity for cell (or bacteria) settling, which takes a long time. In addition, during laminar flow cleaning, the bottom cells (or bacteria) are easily carried away by the cleaning solution, resulting in a low cell retention rate.

[0003] Therefore, it is necessary to provide a new approach to solve the aforementioned technical problems. Utility Model Content

[0004] In order to achieve the above-mentioned objectives and other advantages of this utility model, the purpose of this utility model is to provide a cell well plate, which includes a base layer, an intermediate layer and a hydrophobic layer from bottom to top. The intermediate layer has a plurality of sample wells, which are connected to the base layer. An electrode is disposed on the base layer and the electrode is located below the sample well.

[0005] The electrode is used to generate a non-uniform electric field, causing cells or bacteria to move toward one side of the non-uniform electric field and settle at the bottom of the sample well.

[0006] The intermediate layer is used to clean the sample solution;

[0007] The hydrophobic layer is used to prevent the sample from flowing through or connecting within the sample wells and between sample wells.

[0008] Furthermore, the electrode is printed on the upper surface of the substrate layer.

[0009] Furthermore, the number of electrodes is two.

[0010] Furthermore, the two electrodes are arranged in a tooth-like pattern.

[0011] Furthermore, the frequency, voltage, and spacing of the electrodes are determined according to the components to be enriched in the sample.

[0012] Furthermore, a washing hole is also provided on the periphery of the sample hole, the washing hole is interconnected with the sample hole, and the depth of the washing hole is less than the depth of the sample hole.

[0013] Furthermore, the number of the washing holes is two.

[0014] Furthermore, the two washing holes are symmetrically arranged on both sides of the sample hole.

[0015] Furthermore, during cleaning, each of the cleaning holes is equipped with a nozzle for adding and draining liquid, respectively.

[0016] Compared with the prior art, the beneficial effects of this utility model are:

[0017] This invention provides a cell well plate that increases cell sedimentation speed by applying an electric field below the well plate and using dielectrophoresis force. At the same time, the cells (or bacteria) are less likely to be lost after sedimentation due to dielectrophoresis force, resulting in better retention during laminar flow washing.

[0018] The above description is merely an overview of the technical solution of this utility model. In order to better understand the technical means of this utility model and to implement it according to the contents of the specification, the preferred embodiments of this utility model are described in detail below with reference to the accompanying drawings. The specific implementation methods of this utility model are given in detail in the following embodiments and their accompanying drawings. Attached Figure Description

[0019] The accompanying drawings, which are included to provide a further understanding of the present invention and form part of this application, illustrate exemplary embodiments of the present invention and, together with the description thereof, serve to explain the present invention and do not constitute an undue limitation thereof. In the drawings:

[0020] Figure 1 Diagram of the cell well plate structure;

[0021] Figure 2 This is a schematic diagram of the electrode layout;

[0022] Figure 3 This is a schematic diagram of cell sedimentation.

[0023] Figure 4 This is a schematic diagram of the cleaning process.

[0024] In the figure, 1 is the base layer; 11 is the electrode; 2 is the intermediate layer; 21 is the sample well; 22 is the washing well; and 3 is the hydrophobic layer. Detailed Implementation

[0025] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present invention.

[0026] In the accompanying drawings, shapes and dimensions may be enlarged for clarity, and the same reference numerals will be used in all figures to indicate the same or similar parts.

[0027] In the following description, terms such as center, thickness, height, length, front, back, rear, left, right, top, bottom, upper, and lower are defined relative to the structure shown in the accompanying drawings. In particular, "height" corresponds to the dimension from top to bottom, "width" corresponds to the dimension from left to right, and "depth" corresponds to the dimension from front to back. These are relative concepts and may vary depending on their location and usage. Therefore, these or other orientations should not be interpreted as restrictive terms.

[0028] Terms involving attachment, connection, etc. (e.g., “connection” and “attachment”) refer to the relationship in which these structures are directly or indirectly fixed or attached to each other through an intermediate structure, as well as movable or rigid attachments or relationships, unless otherwise explicitly stated.

[0029] Example 1

[0030] A type of cell plate, such as Figure 1 As shown, from bottom to top, it includes a base layer 1, an intermediate layer 2, and a hydrophobic layer 3. The intermediate layer 2 has multiple sample holes 21, which are connected to the base layer 1. An electrode 11 is disposed on the base layer 1, and the electrode 11 is located below the sample holes 21.

[0031] The electrode is used to generate a non-uniform electric field, causing cells or bacteria to move toward one side of the non-uniform electric field and settle at the bottom of the sample well; that is, the method of dielectrophoresis is used to accelerate the sedimentation of particles.

[0032] The intermediate layer is used to clean the sample solution;

[0033] The hydrophobic layer is used to prevent the sample from flowing through or connecting within the sample wells and between sample wells.

[0034] In this embodiment, the hydrophobic layer is made of a material with strong hydrophobic properties, which can keep the sample inside the pores and prevent cross-flow between the pores.

[0035] In some embodiments, the electrodes are printed on the upper surface of the substrate layer. Further, there are two electrodes, which are arranged in a toothed pattern to generate a non-uniform electric field, specifically as follows: Figure 2 As shown, cells (or bacteria) will induce positive and negative charges in a dielectric field, and under the influence of a non-uniform electric field, they will move towards one side of the non-uniform electric field.

[0036] In some embodiments, such as Figure 3As shown, a washing hole 22 is also provided around the sample well 21. The washing hole 22 is interconnected with the sample well 21. The depth of the washing hole 22 is less than the depth of the sample well 21, meaning the bottom of the sample well is lower than the surface of the liquid addition / discharge platform. This allows for a faster flow rate in the upper layer and a slower flow rate in the lower layer, carrying away debris, proteins, and other impurities that have diffused to the upper part of the solution. It also minimizes disturbance to cells (or bacteria) settling at the bottom of the sample well, allowing for greater retention. Furthermore, the number of washing holes is two, such as... Figure 1 , Figure 3 As shown. Two washing holes are symmetrically arranged on both sides of the sample well. During washing, a nozzle is provided above each washing hole for adding and draining liquid, respectively.

[0037] Depending on the components to be enriched in the sample, appropriate frequency, voltage, and electrode spacing are selected so that the desired cells (or bacteria) are enriched at the lower electrode under the action of positive dielectrophoretic force. Under the action of dielectrophoretic force, gravity, and the resistance of buoyancy fluid drag, they move downward, thus obtaining a faster particle settling velocity than when gravity is the only factor.

[0038] After the cells settle to the bottom, when cell washing begins, the dielectrophoretic force exerts a pulling force on the cells. Under the combined influence of dielectrophoretic force and gravity, the cells adhere to the electrode surface, making them less likely to be carried away by the upward-flowing washing solution, thus improving cell retention. Furthermore, the addition of dielectrophoretic force can also increase the washing flow rate, reducing experimental time. The retention rate can be calculated using a hemocytometer, and laminar flow washing can be employed. The principle of the washing process is as follows... Figure 4 As shown. Specifically, the supernatant is diluted by injecting cleaning fluid and then removing the waste fluid to remove impurities; a quasi-static cleaning process is achieved by using a slow flow rate to reduce disturbance to the bacteria at the bottom, thereby preserving the bacteria.

[0039] The cell plate provided in this embodiment can improve the sedimentation rate of cells in the sample solution, reduce experimental time, and improve the cell retention rate during the washing process.

[0040] The number of devices and processing scale described herein are for the purpose of simplifying the description of this utility model. Applications, modifications, and variations of this utility model will be readily apparent to those skilled in the art.

[0041] Although the embodiments of this utility model have been disclosed above, they are not limited to the applications listed in the specification and embodiments. They can be applied to various fields suitable for this utility model. For those skilled in the art, other modifications can be easily made. Therefore, without departing from the general concept defined by the claims and their equivalents, this utility model is not limited to the specific details and the illustrations shown and described herein.

[0042] It should also be noted that the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0043] The various embodiments in this specification are described in a progressive manner. Similar or identical parts between embodiments can be referred to interchangeably. Each embodiment focuses on describing the differences from other embodiments. In particular, the system embodiments are basically similar to the method embodiments, so the description is relatively simple; relevant parts can be referred to the descriptions in the method embodiments.

[0044] The above description is merely an embodiment of this specification and is not intended to limit the scope of one or more embodiments of this specification. Various modifications and variations can be made to one or more embodiments of this specification by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principle of one or more embodiments of this specification should be included within the scope of the claims of one or more embodiments of this specification.

Claims

1. A cell well plate, characterized in that: From bottom to top, it includes a base layer, an intermediate layer, and a hydrophobic layer. The intermediate layer has multiple sample wells that are connected to the base layer. Electrodes are disposed on the base layer and are located below the sample wells. The electrode is used to generate a non-uniform electric field, causing cells or bacteria to move toward one side of the non-uniform electric field and settle at the bottom of the sample well. The intermediate layer is used to clean the sample solution; The hydrophobic layer is used to prevent the sample from flowing through or connecting within the sample wells and between sample wells.

2. The cell plate as described in claim 1, characterized in that: The electrode is printed on the upper surface of the substrate layer.

3. A cell well plate as described in claim 2, characterized in that: The number of electrodes is two.

4. A cell well plate as described in claim 3, characterized in that: The two electrodes are arranged in a tooth-like pattern.

5. A cell well plate as described in claim 1, characterized in that: The frequency, voltage, and spacing of the electrodes are determined according to the components to be enriched in the sample.

6. A cell well plate as described in claim 1, characterized in that: A washing hole is also provided on the periphery of the sample hole. The washing hole is connected to the sample hole, and the depth of the washing hole is less than the depth of the sample hole.

7. A cell well plate as described in claim 6, characterized in that: The number of washing holes is two.

8. A cell plate as described in claim 7, characterized in that: The two washing holes are symmetrically arranged on both sides of the sample hole.

9. A cell plate as described in claim 8, characterized in that: During cleaning, each of the cleaning holes is equipped with a nozzle for adding and draining liquid, respectively.

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