A multi-layer filtration anti-pollution liquid strain inoculation device

Abstract

The utility model provides a kind of multi-layer filtration pollution-preventing liquid strain inoculation device.The multi-layer filtration pollution-preventing liquid strain inoculation device includes: inoculation box;Electric slide rail is fixedly installed on the inner wall on the top of the inoculation box;Electric cylinder is fixedly installed on the electric slide rail output block;Inoculation gun is installed on the electric cylinder output rod;Liquid storage tank is fixedly installed on the top of the inoculation box;Liquid guide pipe is arranged on the liquid storage tank and is communicated with the inoculation gun;Vacuumizing mechanism is installed on the side of the inoculation box for extracting air in the inoculation box;Multi-layer filtration mechanism is installed on the side of the inoculation box for multi-layer filtration of air into the inoculation box.The multi-layer filtration pollution-preventing liquid strain inoculation device provided by the utility model has the advantages of multi-layer filtration of air into the inoculation box, easy operation and high inoculation success rate.

Description

Technical Field

[0001] This utility model relates to the field of liquid microbial inoculation technology, and in particular to a multi-layer filtration and anti-contamination liquid microbial inoculation device. Background Technology

[0002] Liquid spawn inoculation is a crucial step in edible mushroom production, offering advantages such as high inoculation efficiency, rapid mycelial growth, and low contamination rates. Liquid spawn can be directly sprayed or injected into the substrate, eliminating the need for breaking and digging out solid spawn, making it suitable for large-scale mechanized production. The mycelium of liquid spawn is flocculent or spherical, evenly dispersed, and can quickly germinate and cover the substrate surface, shortening the mycelial growth cycle (for example, the growth time for shiitake and enoki mushrooms can be reduced by more than half). Liquid spawn is cultivated under aseptic conditions, minimizing the chance of contamination by other microorganisms during inoculation, achieving a yield rate of 98%-100%. The short production cycle of liquid spawn reduces total investment in facilities by 20%-30%, and eliminates the need for plastic bag packaging, reducing environmental pollution. Inoculation equipment is required during the liquid spawn inoculation process.

[0003] However, existing liquid inoculation devices often lack multi-layer filtration for the air entering the inoculation chamber, so bacteria in the air inside the inoculation chamber can affect the success rate of inoculation.

[0004] Therefore, it is necessary to provide a multi-layer filtration and anti-contamination liquid microbial inoculation device to solve the above-mentioned technical problems. Utility Model Content

[0005] To address the technical problem that existing multi-layer filtration and anti-contamination liquid inoculation devices often lack multi-layer filtration functionality for the air entering the inoculation chamber, this invention provides a multi-layer filtration and anti-contamination liquid inoculation device.

[0006] The multi-layer filtration and anti-contamination liquid inoculation device provided by this utility model includes: an inoculation box; an electric slide rail fixedly installed on the inner wall of the top of the inoculation box; an electric cylinder fixedly installed on the output block of the electric slide rail; an inoculation gun installed on the output rod of the electric cylinder; a storage tank fixedly installed on the top of the inoculation box; a liquid guide pipe provided on the storage tank and connected to the inoculation gun; a vacuum mechanism installed on one side of the inoculation box for extracting air from the inoculation box; and a multi-layer filtration mechanism installed on one side of the inoculation box for multi-layer filtration of the air entering the inoculation box.

[0007] Preferably, the vacuuming mechanism includes: a negative pressure pump fixedly installed on one side of the inoculation box; a negative pressure pipe disposed at the air inlet end of the negative pressure pump and connected to the inoculation box; and a first one-way valve disposed on the negative pressure pipe.

[0008] Preferably, the multi-layer filtration mechanism includes: a gas storage cylinder fixedly installed on one side of the inoculation box; a filter box fixedly installed on one side of the gas storage cylinder; a pre-filter, a medium-efficiency filter, a high-efficiency filter, and an ultra-high-efficiency filter installed inside the filter box; an air pump installed at the bottom of the filter box; an air guide pipe installed on the filter box and connected to the gas storage cylinder; and a second one-way valve installed on the air guide pipe.

[0009] Preferably, the gas storage cylinder is provided with an air inlet pipe, the air inlet pipe is connected to the inoculation box, and the air inlet pipe is provided with a solenoid valve.

[0010] Preferably, a first pressure sensor is provided on the gas storage cylinder, and a second pressure sensor is provided on the inoculation box.

[0011] Preferably, an inoculation tray is provided on the bottom inner wall of the inoculation box, and a sealed box door is provided on the inoculation box.

[0012] Preferably, the sealed box door is provided with an observation window, and the sealed box door is provided with a controller.

[0013] Compared with related technologies, the multi-layer filtration and anti-contamination liquid bacterial inoculation device provided by this utility model has the following beneficial effects:

[0014] This invention provides a multi-layer filtration and contamination-preventing liquid bacterial inoculation device. An electric slide rail drives the inoculation gun to move laterally, while an electric cylinder drives it to move up and down. Liquid bacterial culture from the storage tank enters the inoculation gun along a guide tube, allowing inoculation of the bacterial bag. A vacuum mechanism removes air from the inoculation chamber, and a multi-layer filtration system filters the air entering the chamber in multiple stages to prevent airborne bacteria from affecting the inoculation success rate. A pressure pump and negative pressure pipe remove air from the inoculation chamber, while an air pump injects external air into the filtration chamber, which then passes through a primary filter... Medium-efficiency filter, high-efficiency filter and ultra-high-efficiency filter perform multi-stage filtration of air. The filtered air enters the air storage cylinder through the air guide tube for storage. The clean air in the air storage cylinder, which has undergone multi-stage filtration, is injected into the inoculation box through the air inlet pipe. The opening and closing of the air inlet pipe can be controlled by a solenoid valve. The air pressure in the air storage cylinder can be monitored by a first air pressure sensor. The air pressure in the inoculation box can be monitored by a second air pressure sensor. The inoculation tray can be used to place the inoculation bags. The inoculation box can be sealed by a sealing door. The inoculation process can be observed through an observation window. The device can be operated and controlled by a controller. Attached Figure Description

[0015] Figure 1 A schematic diagram of a preferred embodiment of the multi-layer filtration and anti-contamination liquid bacterial inoculation device provided by this utility model;

[0016] Figure 2 for Figure 1 A schematic diagram of the front view of the structure;

[0017] Figure 3 for Figure 1 The enlarged schematic diagram of part A shown in the figure.

[0018] Numbered in the diagram: 1. Inoculation box; 2. Electric slide rail; 3. Electric cylinder; 4. Inoculation gun; 5. Liquid storage tank; 6. Liquid guide tube; 7. Negative pressure pump; 8. Negative pressure pipe; 9. First one-way valve; 10. Gas storage cylinder; 11. Filter box; 12. Primary filter; 13. Medium efficiency filter; 14. High efficiency filter; 15. Ultra-high efficiency filter; 16. Air pump; 17. Air guide tube; 18. Second one-way valve; 19. Air inlet pipe; 20. Solenoid valve; 21. First air pressure sensor; 22. Second air pressure sensor; 23. Inoculation tray; 24. Sealed box door; 25. Observation window; 26. Controller. Detailed Implementation

[0019] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0020] Please refer to the following: Figure 1-3 ,in, Figure 1 A schematic diagram of a preferred embodiment of the multi-layer filtration and anti-contamination liquid bacterial inoculation device provided by this utility model; Figure 2 for Figure 1 A schematic diagram of the front view of the structure; Figure 3 for Figure 1 The enlarged schematic diagram of part A shown in the figure. The multi-layered filtration and contamination-preventing liquid inoculation device includes: an inoculation box 1; an electric slide rail 2 (model KCB17) fixedly installed on the inner wall of the top of the inoculation box 1; an electric cylinder 3 (model NKLA64) fixedly installed on the output block of the electric slide rail 2; an inoculation gun 4 installed on the output rod of the electric cylinder 3; a liquid storage tank 5 fixedly installed on the top of the inoculation box 1; a liquid guide tube 6 disposed on the liquid storage tank 5 and connected to the inoculation gun 4; and a device installed on one side of the inoculation box 1 for extracting the empty liquid inside the inoculation box 1. The system includes a vacuum mechanism for air extraction and a multi-layer filtration mechanism installed on one side of the inoculation box 1 for filtering the air entering the box. The inoculation gun 4 can be moved laterally via an electric slide rail 2 and moved up and down via an electric cylinder 3. The liquid inoculum in the storage tank 5 enters the inoculation gun 4 along the liquid guide tube 6. The inoculation gun 4 can be used to inoculate the bacterial pack. The vacuum mechanism can remove the air from the inoculation box 1, and the multi-layer filtration mechanism can perform multi-stage filtration of the air entering the inoculation box 1 to prevent bacteria in the air from affecting the success rate of inoculation.

[0021] The vacuuming mechanism includes: a negative pressure pump 7 fixedly installed on one side of the inoculation box 1; a negative pressure pipe 8 disposed at the air inlet of the negative pressure pump 7 and connected to the inoculation box 1; and a first one-way valve 9 disposed on the negative pressure pipe 8, which extracts air from the inoculation box 1 through the pump 7 and the negative pressure pipe 8.

[0022] The multi-layer filtration mechanism includes: a gas storage cylinder 10 fixedly installed on one side of the inoculation box 1; a filter box 11 fixedly installed on one side of the gas storage cylinder 10; a pre-filter 12, a medium-efficiency filter 13, a high-efficiency filter 14, and an ultra-high-efficiency filter 15 installed inside the filter box 11; an air pump 16 installed at the bottom of the filter box 11, the air pump 16 being a HIBLOW HP-20; an air guide pipe 17 installed on the filter box 11 and connected to the gas storage cylinder 10; and a second one-way valve 18 installed on the air guide pipe 17. External air is injected into the filter box 11 by the air pump 16, and the air is filtered through the pre-filter 12, the medium-efficiency filter 13, the high-efficiency filter 14, and the ultra-high-efficiency filter 15. The filtered air then enters the gas storage cylinder 10 for storage along the air guide pipe 17.

[0023] The gas cylinder 10 is equipped with an air inlet pipe 19, which is connected to the inoculation box 1. The air inlet pipe 19 is equipped with a solenoid valve 20. Clean air that has undergone multi-stage filtration in the gas cylinder 10 is injected into the inoculation box 1 through the air inlet pipe 19. The solenoid valve 20 can control the opening and closing of the air inlet pipe 19.

[0024] A first pressure sensor 21 is installed on the gas storage cylinder 10, and a second pressure sensor 22 is installed on the inoculation box 1. The first pressure sensor 21 and the second pressure sensor 22 are both BMP280. The first pressure sensor 21 can monitor the pressure inside the gas storage cylinder 10, and the second pressure sensor 22 can monitor the pressure inside the inoculation box 1.

[0025] An inoculation tray 23 is provided on the bottom inner wall of the inoculation box 1, and a sealing door 24 is provided on the inoculation box 1. The inoculation tray 23 can be used to place the inoculation bags, and the sealing door 24 can be used to seal the inoculation box 1.

[0026] An observation window 25 is provided on the sealed box door 24, and a controller 26 is provided on the sealed box door 24. The controller 26 is model ZG-ASLM. The inoculation process can be observed through the observation window 25, and the device can be operated and controlled through the controller 26.

[0027] The working principle of the multi-layer filtration and anti-contamination liquid bacterial inoculation device provided by this utility model is as follows:

[0028] External air is injected into the filter box 11 by air pump 16. The air is filtered through a multi-stage filter 12, a medium-efficiency filter 13, a high-efficiency filter 14, and an ultra-high-efficiency filter 15. The filtered air enters the gas storage bottle 10 through the air guide pipe 17 for storage. The sterilized bacterial pack is placed into the inoculation tray 23. The inoculation box 1 is sealed through the sealing door 24. The negative pressure pump 7 is started. The negative pressure pump 7 extracts the air from the inoculation box 1 through the negative pressure pipe 8. The solenoid valve 20 is opened. The clean air that has undergone multi-stage filtration in the gas storage bottle 10 is injected into the inoculation box 1 through the air inlet pipe 19, thereby avoiding the influence of bacteria in the air on the inoculation success rate.

[0029] The electric slide rail 2 can drive the inoculation gun 4 to move horizontally, and the electric cylinder 3 can drive the inoculation gun 4 to move up and down. The liquid inoculum in the storage tank 5 enters the inoculation gun 4 along the liquid guide tube 6, and the inoculation gun 4 can be used to inoculate the inoculum pack.

[0030] Compared with related technologies, the multi-layer filtration and anti-contamination liquid bacterial inoculation device provided by this utility model has the following beneficial effects:

[0031] This invention provides a multi-layered filtration and pollution-preventing liquid bacterial inoculation device. An electric slide rail 2 drives the inoculation gun 4 to move laterally, and an electric cylinder 3 drives the inoculation gun 4 to move vertically. Liquid bacterial culture from the storage tank 5 enters the inoculation gun 4 along the liquid guide tube 6, inoculating the bacterial pack. A vacuum mechanism removes air from the inoculation chamber 1, and a multi-layered filtration mechanism filters the air entering the inoculation chamber 1 in multiple stages to prevent airborne bacteria from affecting the inoculation success rate. A pressure pump 7 and a negative pressure pipe 8 remove air from the inoculation chamber 1, and an air pump 16 injects external air into the filter chamber 11, which then passes through a primary filter 12 and a secondary filter 13. High-efficiency filter 14 and ultra-high-efficiency filter 15 perform multi-stage filtration of air. The filtered air enters the gas storage cylinder 10 for storage through the air guide pipe 17. The clean air that has undergone multi-stage filtration in the gas storage cylinder 10 is injected into the inoculation box 1 through the air inlet pipe 19. The opening and closing of the air inlet pipe 19 can be controlled by the solenoid valve 20. The air pressure in the gas storage cylinder 10 can be monitored by the first air pressure sensor 21, and the air pressure in the inoculation box 1 can be monitored by the second air pressure sensor 22. The inoculation tray 23 can be used to place the inoculation bag. The inoculation box 1 can be sealed by the sealing door 24. The inoculation process can be observed through the observation window 25. The device can be operated and controlled by the controller 26.

[0032] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the content of this utility model specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.

Claims

1. A multi-layer filtration and contamination-preventing liquid bacterial inoculation device, characterized in that, include: Inoculation box; An electric slide rail is fixedly installed on the inner wall of the top of the inoculation box; An electric cylinder fixedly mounted on the electric slide rail output block; The inoculation gun is mounted on the output rod of the electric cylinder; A liquid storage tank fixedly installed on top of the inoculation box; A liquid guide tube is installed on the liquid storage tank and connected to the inoculation gun; A vacuuming mechanism installed on one side of the inoculation box for removing air from inside the inoculation box; A multi-layer filtration mechanism is installed on one side of the inoculation box to perform multi-layer filtration of the air entering the inoculation box.

2. The multi-layer filtration and anti-contamination liquid bacterial inoculation device according to claim 1, characterized in that, The vacuum pumping mechanism includes: A negative pressure pump is fixedly installed on one side of the inoculation box; A negative pressure pipe is installed at the air inlet of the negative pressure pump and connected to the inoculation box; The first check valve is installed on the negative pressure pipe.

3. The multi-layer filtration and anti-contamination liquid bacterial inoculation device according to claim 1, characterized in that, The multi-layer filtration mechanism includes: A gas cylinder is fixedly installed on one side of the inoculation box; A filter box is fixedly installed on one side of the gas storage cylinder; The pre-filter, medium-efficiency filter, high-efficiency filter, and ultra-high-efficiency filter are installed inside the filter box; An air pump installed at the bottom of the filter box; A gas guide pipe installed on the filter box and connected to the gas storage cylinder; A second one-way valve is installed on the air duct.

4. The multi-layer filtration and anti-contamination liquid bacterial inoculation device according to claim 3, characterized in that, The gas cylinder is equipped with an air inlet pipe, which is connected to the inoculation box, and a solenoid valve is installed on the air inlet pipe.

5. The multi-layer filtration and anti-contamination liquid bacterial inoculation device according to claim 3, characterized in that, The gas storage cylinder is equipped with a first pressure sensor, and the inoculation box is equipped with a second pressure sensor.

6. The multi-layer filtration and anti-contamination liquid bacterial inoculation device according to claim 1, characterized in that, An inoculation tray is provided on the bottom inner wall of the inoculation box, and a sealed box door is provided on the inoculation box.

7. The multi-layer filtration and anti-contamination liquid bacterial inoculation device according to claim 6, characterized in that, An observation window is provided on the sealed box door, and a controller is provided on the sealed box door.

Images