Mesenchymal stem cell hypoxic culture device

Abstract

This utility model belongs to the technical field of cell culture equipment, specifically relating to a mesenchymal stem cell hypoxia culture device. It includes a culture chamber, which consists of an outer door, an inner partition door, a chamber body, and a shelf. The chamber body has a hollow structure, and the shelf is installed inside the chamber. The inner partition door is connected to both sides of the front end of the chamber body and closes it. The outer door is connected to the chamber body and closes the inner partition door. The sides of the chamber body also have connecting holes that penetrate the cavity. A gas filter is installed on the pipe connected to the connecting holes. The bottom of the chamber body also has a flow guide mechanism for collecting condensate. This utility model's mesenchymal stem cell hypoxia culture device reduces the impact of opening and closing the door on the culture environment by using a partitioned cabinet door, while simultaneously disinfecting and filtering the gas entering the culture chamber, thereby improving the proliferation rate of stem cell culture.

Description

Technical Field

[0001] This utility model belongs to the field of cell culture equipment technology, specifically relating to a mesenchymal stem cell hypoxia culture device. Background Technology

[0002] Mesenchymal stem cells (MSCs) are a widely available type of adult stem cell with self-renewal and multi-lineage differentiation capabilities. They possess all the common characteristics of stem cells, namely self-renewal and multi-lineage differentiation, and are commonly used in tissue engineering, regenerative medicine, and immunomodulation. Clinical applications are also the most numerous; their combined use with hematopoietic stem cells can improve transplant success rates and accelerate hematopoietic reconstitution. When patients receive high-dose chemotherapy, infusing MSCs and hematopoietic stem cells together can significantly shorten the patient's blood cell recovery time, and is safe with no adverse reactions. To ensure that the cultured MSCs have good proliferative capacity, differentiation potential, and biocompatibility, strict adherence to standardized operating procedures is necessary during the culture process to improve the success rate and ensure cell quality and experimental reliability.

[0003] Chinese patent CN218435777U discloses a cell culture chamber for anti-tumor experiments, comprising a main body of the chamber, a protective door at the front end of the main body, and a sealing door. Three sets of symmetrically arranged support plates are fixed to the inner wall of the main body. A placement plate is slidably connected to the top of each support plate, and the top of the placement plate has several placement grooves evenly distributed. In this anti-tumor cell culture chamber, when cell culture dishes located inside the main body need to be retrieved, the placement plate is pulled outwards, and the experimenter can then retrieve the cell culture dishes from top to bottom from the top of the placement plate. This minimizes contact with other cell culture dishes and makes retrieval more convenient. Simultaneously, placing the cell culture dishes in the placement grooves reduces the probability of slippage and simplifies the operation of the CO2 cell culture chamber. However, in this patent, the protective door and sealing door are both integral structures. Opening the door allows a large amount of air to enter from the outside, instantly altering the air composition inside the chamber and affecting the cell culture environment. Utility Model Content

[0004] The purpose of this invention is to address the problems existing in the background art by providing a mesenchymal stem cell hypoxia culture device. This device reduces the impact of opening and closing the door on the culture environment by setting up a partitioned cabinet door, and at the same time disinfects and filters the gas entering the culture chamber, thereby improving the proliferation rate of stem cell culture.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a mesenchymal stem cell hypoxia culture device, comprising a culture chamber, the culture chamber consisting of an outer door, an inner partition door, a chamber body, and a shelf. The chamber body has a hollow structure, the shelf is installed inside the chamber body, the inner partition door is connected to both sides of the front end of the chamber body and closes the chamber body, the outer door is connected to the chamber body and closes the inner partition door, and the two sides of the chamber body are also provided with connecting holes that penetrate the cavity, through which carbon dioxide gas is introduced. One connecting hole is connected to an external gas generator through a pipe, and the other connecting hole is connected to an external gas circulator to control the internal carbon dioxide concentration. A gas filter is also provided on the pipe of the external gas generator, and a guide mechanism for collecting condensate is also provided at the bottom of the chamber body.

[0006] Furthermore, the gas filter includes an inlet pipe, an outlet pipe, a filter box, a partition, a filling layer, a filter layer, and a sterilization layer. The inlet pipe is connected to the lower left side of the filter box, and the outlet pipe is connected to the top left side of the filter box. The partition divides the filter box into three areas: the upper left, the lower left, and the right. The sterilization layer, the filling layer, and the filter layer are respectively provided in the three areas, so that the gas passes through the filling layer, the filter layer, and the sterilization layer in sequence from the inlet pipe to the outlet pipe. Correspondingly, three drawers for changing materials are also provided on the filter box.

[0007] Furthermore, the flow guiding mechanism includes an inclined installation of a flow guiding plate and a water collection tank that receives the flow guiding plate. The flow guiding plate is fixedly connected to the bottom of the box body, inclined laterally towards the center and longitudinally towards the front end. The flow guiding plate is connected to the water collection tank at a low position on the right side to collect condensate. The water collection tank is installed at the bottom of the box body by screws.

[0008] Furthermore, the shelf includes a connecting plate installed inside the box, fastening plates located on the front and rear sides of the connecting plate, a sliding plate vertically connected to the two fastening plates, a reinforcing plate and a mesh plate connected between the two sliding plates. The connecting plate is vertically fixed in the middle of both sides of the box. The fastening plates are fixed in the box, one in front and one behind, parallel to the connecting plate. A set of slots is vertically opened on the fastening plates. The sliding plates are longitudinally mounted on the front and rear fastening plates, and their two ends are inserted into the fastening plates through the slots. Sliding plates are symmetrically installed on the side walls of the box. A mesh plate is horizontally connected between the two sliding plates. The mesh plate is slidably connected to the sliding plates along the longitudinal direction. A reinforcing plate for reinforcement is also horizontally connected at the bottom of the two sliding plates.

[0009] Furthermore, a set of guide plates are also spaced apart in the filling layer. The front and rear sides of the guide plates are fixed to the filter box. Adjacent guide plates are staggered. Non-woven fabric and activated carbon are alternately filled between the two guide plates. The sterilization layer is filled with polyethersulfone sterilization filter element.

[0010] Furthermore, filter screens are installed at the connection points between the air inlet pipe and the air outlet pipe and the filter box, and a high-density sponge layer is also provided at the bottom of the filter box to connect the filling layer with the bottom of the filter layer.

[0011] Furthermore, the inner partition door is divided into four small door panels, one each on the top, bottom, left, and right, which enclose the shelf inside the box. Each door panel has a baffle installed at its edge.

[0012] Furthermore, a baffle plate is also provided on the front end face of the guide plate.

[0013] The beneficial effects of this utility model are: 1) This utility model is equipped with a gas filter, which divides the internal flow channel into multiple areas through partitions and guide plates, and fills them with materials for gas filtration. After the gas passes through the flow channel, it completes the drying, filtration and disinfection process, providing dry and sterile gas to the chamber for cultivation. This facilitates the control of the gas environment in the incubator and improves the proliferation rate of stem cells.

[0014] 2) A flow guiding mechanism is set up to guide condensate or other liquids into the water collection tank through the flow guide plate. The inclined setting of the flow guide plate can accelerate the flow of liquid. The baffle plate installed at the front end of the flow guide plate can prevent the liquid from flowing around, which facilitates daily cleaning of the incubator and improves the water collection efficiency. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of this utility model.

[0016] Figure 2 This is a diagram of the internal structure of this utility model.

[0017] Figure 3 For this Figure 2 Enlarged image.

[0018] Figure 4 This is a schematic diagram of the gas filter in this utility model.

[0019] Figure 5 This is a schematic diagram of the flow guiding mechanism in this utility model.

[0020] Figure 6 This is a schematic diagram of the structure of the storage rack in this utility model.

[0021] In the diagram: 1. Incubator; 2. Outer door; 3. Inner partition door; 4. Box body; 5. Shelf; 6. Connecting hole; 7. Pipe; 8. Gas filter; 9. Flow guiding mechanism; 8-1. Air inlet pipe; 8-2. Air outlet pipe; 8-3. Filter box; 8-4. Partition; 8-5. Filling layer; 8-6. Filter layer; 8-7. Sterilization layer; 8-8. Drawer; 9-1. Flow guide plate; 9-2. Water collection tank; 5-1. Connecting plate; 5-2. Fastening plate; 5-3. Slide plate; 5-4. Reinforcing plate; 5-5. Groove; 10. Guide plate; 11. Filter screen; 12. High-density sponge layer; 13. Door panel; 14. Baffle plate; 15. Water baffle plate. Detailed Implementation

[0022] The present invention will be further explained below with reference to the accompanying drawings and specific embodiments.

[0023] Example:

[0024] like Figures 1 to 6 As shown, the mesenchymal stem cell hypoxia culture device of this utility model includes a culture box 1. The culture box 1 is composed of an outer door 2, an inner partition door 3, a box body 4, and a shelf 5. The box body 4 has a cavity structure. The shelf 5 is installed inside the box body 4. The inner partition door 3 is connected to both sides of the front end of the box body 4 and closes the box body 4. The outer door 2 is connected to the box body 4 and closes the inner partition door 3. The box body 4 is also provided with connecting holes 6 on both sides, which are connected to the cavity. Carbon dioxide gas is introduced into the box body 4 through the connecting holes 6. One connecting hole 6 is connected to an external gas generator through a pipe 7, and the other connecting hole 6 is connected to an external gas circulator to control the internal carbon dioxide concentration. A gas filter 8 is also provided on the pipe 7 of the external gas generator. The bottom of the box body 4 is also provided with a guide mechanism 9 for collecting condensate.

[0025] The gas filter 8 includes an inlet pipe 8-1, an outlet pipe 8-2, a filter box 8-3, a partition 8-4, a filling layer 8-5, a filter layer 8-6, and a sterilization layer 8-7. The inlet pipe 8-1 is connected to the lower left side of the filter box 8-3, and the outlet pipe 8-2 is connected to the top left side of the filter box 8-3. The partition 8-4 divides the filter box 8-3 into three areas: the upper left, the lower left, and the right. The sterilization layer 8-7, the filling layer 8-5, and the filter layer 8-6 are respectively installed in the three areas. Layer 8-6 allows gas to pass sequentially from the inlet pipe 8-1 through the filling layer 8-5, the filter layer 8-6, and the sterilization layer 8-7 before reaching the outlet pipe 8-2. Correspondingly, three drawers 8-8 for changing materials are also provided on the filter box 8-3. Filter screens 11 are installed at the connection between the inlet pipe 8-1 and the outlet pipe 8-2 and the filter box 8-3. A high-density sponge layer 12 is also provided at the bottom of the filter box 8-3, connecting the bottom of the filling layer 8-5 and the filter layer 8-6.

[0026] refer to Figure 4In this embodiment, partitions 8-4 are fixedly connected to the front and rear sides of the filter box 8-3, dividing the filter box 8-3 into multiple areas. Materials are filled in the filter box to allow gas to pass through the flow channels, completing the drying, filtration, and sterilization processes, and providing dry, sterile gas to the chamber 4 for cultivation. Simultaneously, removable drawers 8-8 are installed along the partitions 8-4 for easy replacement of the filling materials in each area, facilitating daily cleaning. The high-density sponge layer 12 helps absorb residual moisture in the gas. The sponge itself is highly elastic, allowing for manual removal and replacement of the drawer 8-8 from the inside. For low-oxygen cultivation environments, the input gas is generally carbon dioxide, which is denser than air and tends to settle and accumulate when flowing within the filter box 8-3. Therefore, inputting gas from the bottom and outputting gas from the top, allowing the gas flow channels to move alternately up and down within the filling layer 8-5 and from bottom to top in the process layer, increases the gas flow length and improves the drying and filtration effect.

[0027] The flow guiding mechanism 9 includes a drawer 9-1 and a water collection trough 9-2 that is inclinedly installed on the drawer 9-1 and receives the flow guiding plate 9-1. The flow guiding plate 9-1 is fixedly connected to the bottom of the cabinet 4, inclined horizontally towards the center and vertically towards the front end. The lower right side of the flow guiding plate 9-1 is connected to the water collection trough 9-2 to collect condensate. The water collection trough 9-2 is installed at the bottom of the cabinet 4 with screws. A baffle plate 15 is also provided on the front end face of the flow guiding plate 9-1.

[0028] refer to Figure 5 In this embodiment, the guide plate 9-1 directs condensate or other liquids to the water collection tank 9-2. The inclined setting of the guide plate 9-1 can accelerate the flow of liquid. The baffle plate 15 installed at the front end of the guide plate 9-1 can prevent the liquid from flowing around, making it easier to clean the incubator 1 daily and improving the water collection efficiency.

[0029] refer to Figure 5 The shelf 5 includes a connecting plate 5-1 installed inside the box body 4, a snap-fit ​​plate 5-2 located on the front and rear sides of the connecting plate 5-1, a sliding plate 5-3 vertically connected to the two snap-fit ​​plates 5-2, a reinforcing plate 5-4 connected between the two sliding plates 5-3, and a mesh plate. The connecting plate 5-1 is vertically fixed in the middle of both sides of the box body 4. The snap-fit ​​plates 5-2 are fixed in the box body 4 parallel to the connecting plate 5-1, one in front and one behind. A set of slots 5-5 are vertically opened on the snap-fit ​​plates 5-2. The sliding plates 5-3 are longitudinally mounted on the two snap-fit ​​plates 5-2, and both ends are inserted into the snap-fit ​​plates 5-2 through the slots 5-5. The sliding plates 5-3 are symmetrically installed on the two side walls of the box body 4. A mesh plate is horizontally connected between the two sliding plates 5-3. The mesh plate is slidably connected to the sliding plates 5-3 along the longitudinal direction for placing culture medium. A reinforcing plate 5-4 is also horizontally connected to the bottom of the two sliding plates 5-3 for reinforcement.

[0030] refer to Figure 5In this embodiment, the stencil can be pulled outward or inserted inward via the sliding plate 5-3, facilitating the removal or insertion of culture medium for operation. The two ends of the sliding plate 5-3, which supports the stencil, are inserted into the slots 5-5 on the fastening plate 5-2. By changing the insertion position, the spacing between two adjacent stencils can be increased or decreased, making it easier to place culture medium of different heights and sizes for cultivation. The reinforcing plate 5-4 is used to support the stencil, preventing it from sagging and deforming under stress, and improving structural strength. Similarly, the sliding plates 5-3 on the left and right sides of the stencil can be staggered to create an inclined stencil surface, suitable for different cultivation environments, thus improving practicality.

[0031] A set of guide plates 10 are also arranged at intervals in the filling layer 8-5. The front and rear sides of the guide plates 10 are fixed on the filter box 8-3. The two adjacent guide plates 10 are arranged alternately. Non-woven fabric and activated carbon are alternately filled between the two guide plates 10. The sterilization layer 8-7 is filled with polyethersulfone sterilization filter element.

[0032] refer to Figure 4 In this embodiment, the gas flow length during air intake is increased by the guide plate 10. Since carbon dioxide is heavier than air, it will accumulate downwards during air intake. Therefore, the guide plates 10 are set up vertically to separate the filling layer 8-5, which can force the carbon dioxide to flow up and down in the filling layer 8-5, thereby improving the drying and water absorption effect. After passing through the filter layer 8-6 to remove impurities, and the sterilization layer 8-7 to disinfect and sterilize, the gas flows out. The gas enters from the bottom and exits from the top, which can maximize the drying, filtration and sterilization of the gas.

[0033] The inner partition door is divided into four small unit door panels 13, one each on the top, bottom, left, and right, which enclose the shelf 5 inside the box 4. Each door panel 13 has a cover plate 14 installed on its edge.

[0034] refer to Figure 2 In this embodiment, all four door panels 13 are rotatably connected to the chamber 4 via hinges, and anti-collision pads are fixedly installed on the door panels 13 away from the hinges. Door closing buffers are installed inside the corresponding chamber 4. When the door panels 13 are closed, the anti-collision pads contact the door closing buffers to reduce collision wear, and the closing is quiet and shockproof. The upper and lower door panels 13 are a group and are enclosed in the outer door 2 at one end. A baffle 14 is installed in the gap between the upper and lower door panels 13 to minimize the opening area and prevent gas from mixing in and affecting the culture environment. The four door panels 13 are divided independently on the left and right sides. By opening the outer door 2 to the left or right, half of the inner partition door is exposed. Then, the upper or lower door panel 13 is opened to expose the shelf 5 inside the chamber 4 for taking and putting in items, reducing gas loss or diffusion.

[0035] This invention incorporates a gas filter, dividing the internal flow channel into multiple areas using partitions and guide plates, and filling them with materials for gas filtration. This allows the gas to undergo drying, filtration, and sterilization processes after passing through the flow channel, providing dry and sterile gas to the incubator for cultivation. This facilitates control of the gas environment within the incubator and improves the stem cell proliferation rate. A flow guiding mechanism is also included, using guide plates to direct condensate or other liquids into a collection tank. The inclined design of the guide plates accelerates liquid flow, and a baffle plate at the front of the guide plates prevents liquid from flowing around, facilitating daily cleaning of the incubator and improving water collection efficiency.

[0036] The above description is only used to illustrate the technical solution of this utility model and is not intended to limit it. Any other modifications or equivalent substitutions made by those skilled in the art to the technical solution of this utility model, as long as they do not depart from the spirit and scope of the technical solution of this utility model, should be covered within the scope of the claims of this utility model.

Claims

1. A hypoxic culture device for mesenchymal stem cells, characterized in that: The incubator comprises an outer door, an inner partition door, a box body, and a shelf. The box body has a hollow structure, and the shelf is installed inside the box body. The inner partition door is connected to both sides of the front end of the box body and closes the box body. The outer door is connected to the box body and closes the inner partition door. The box body also has connecting holes on both sides that communicate with the hollow cavity. Carbon dioxide gas is introduced into the box body through the connecting holes. One connecting hole is connected to an external gas generator through a pipe, and the other connecting hole is connected to an external gas circulator to control the internal carbon dioxide concentration. A gas filter is also installed on the pipe of the external gas generator. The bottom of the box body is also equipped with a guide mechanism for collecting condensate.

2. The mesenchymal stem cell hypoxia culture device according to claim 1, characterized in that: The gas filter includes an inlet pipe, an outlet pipe, a filter box, a partition, a filling layer, a filter layer, and a sterilization layer. The inlet pipe is connected to the lower left side of the filter box, and the outlet pipe is connected to the top left side of the filter box. The partition divides the filter box into three areas: the upper left, the lower left, and the right. The sterilization layer, the filling layer, and the filter layer are respectively set in the three areas, so that the gas passes through the filling layer, the filter layer, and the sterilization layer in sequence from the inlet pipe to the outlet pipe. Correspondingly, three drawers for changing materials are also provided on the filter box.

3. The mesenchymal stem cell hypoxia culture device according to claim 1, characterized in that: The flow guiding mechanism includes a flow guiding plate and a water collection tank that are inclinedly installed on the flow guiding plate. The flow guiding plate is fixedly connected to the bottom of the box body, inclined laterally towards the center and longitudinally towards the front end. The flow guiding plate is connected to the water collection tank at the lower right side to collect condensate. The water collection tank is installed at the bottom of the box body by screws.

4. The mesenchymal stem cell hypoxia culture device according to claim 1, characterized in that: The shelf includes a connecting plate installed inside the box, fastening plates located on the front and rear sides of the connecting plate, a sliding plate vertically connected to the two fastening plates, a reinforcing plate and a mesh plate connected between the two sliding plates. The connecting plate is vertically fixed in the middle of both sides of the box. The fastening plates are fixed in the box, one in front and one behind, parallel to the connecting plate. A set of slots is vertically opened on the fastening plates. The sliding plates are longitudinally mounted on the front and rear fastening plates, and their two ends are inserted into the fastening plates through the slots. Sliding plates are symmetrically installed on the side walls of the box. A mesh plate is horizontally connected between the two sliding plates. The mesh plate is slidably connected to the sliding plates along the longitudinal direction. A reinforcing plate for reinforcement is also horizontally connected at the bottom of the two sliding plates.

5. The mesenchymal stem cell hypoxia culture device according to claim 2, characterized in that: The filling layer is also provided with a set of guide plates at intervals. The front and rear sides of the guide plates are fixed on the filter box. Adjacent guide plates are staggered. Non-woven fabric and activated carbon are alternately filled between the two guide plates. The sterilization layer is filled with polyethersulfone sterilization filter element.

6. The mesenchymal stem cell hypoxia culture device according to claim 2, characterized in that: The air inlet and outlet pipes are connected to the filter box with filter screens. A high-density sponge layer is also provided at the bottom of the filter box to connect the filling layer with the bottom of the filter layer.

7. The mesenchymal stem cell hypoxia culture device according to claim 1, characterized in that: The inner partition door is divided into four small door panels, one each on the top, bottom, left, and right sides, which enclose the shelf inside the box. Each door panel has a baffle installed at its edge.

8. The mesenchymal stem cell hypoxia culture device according to claim 3, characterized in that: A baffle plate is also provided on the front end face of the guide plate.

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