SPR chip storage box

Abstract

This utility model discloses an SPR chip storage box, including a base, a top cover, and a silicone sleeve. The base and top cover are closable, forming a cavity for accommodating the SPR chip when closed. The silicone sleeve is fitted inside the through-hole of the top cover, with its internal cavity corresponding to the bioactive surface of the biochip. The base has a flexible raised ring structure that supports the edge of the non-bioactive surface of the biochip, ensuring a sealed contact between the edge of the bioactive surface and the silicone sleeve. The internal cavity of the silicone sleeve contains pure water, which directly contacts the bioactive surface of the biochip while isolating the pure water from the adhesive strip and carrier of the SPR chip. This utility model effectively prevents the adhesive strip from detaching and becoming contaminated by isolating the pure water from the adhesive strip and carrier. The flexible raised ring structure ensures a reliable seal, and the simple structure makes it suitable for short-term or long-term storage of SPR chips.

Description

Technical Field

[0001] This utility model relates to the field of storage box technology, specifically to an SPR chip storage box. Background Technology

[0002] Surface plasmon resonance (SPR) chips are widely used in the analysis of biomolecular interactions. Their core sensitive region is typically composed of a metal thin film and a functionalized biomolecular layer, making them extremely sensitive to environmental conditions. Currently, the preservation methods for SPR chips are relatively primitive, mostly involving directly immersing the chips in the filling liquid within centrifuge tubes or EP tubes.

[0003] SPR chips typically consist of a biochip carrier, a biochip, and adhesive strips. One side of the biochip is a bioactive surface, and the other side is a non-bioactive surface. To maintain the bioactivity of the bioactive surface during preservation, current conventional preservation methods often employ liquid immersion.

[0004] However, this method has the following shortcomings:

[0005] First, the adhesive strips are prone to detachment after prolonged soaking, causing the biochip to separate from the carrier and affecting chip performance.

[0006] Second, the biochip carrier may be contaminated by contact with hands or tweezers, and harmful substances in the adhesive strip may also leach out during the soaking process, thus contaminating the biochip.

[0007] Therefore, there is an urgent need to develop an SPR chip storage box to solve problems such as adhesive strip detachment and contamination risks in existing storage methods. Utility Model Content

[0008] To address the aforementioned technical problems, this utility model proposes an SPR chip storage box.

[0009] To achieve the above objectives, the technical solution of this utility model is as follows:

[0010] This utility model discloses an SPR chip storage box for storing SPR chips. The SPR chip includes: a biochip carrier, a biochip, and an adhesive strip. The adhesive strip is used to bond and fix the edge of the biochip to the biochip carrier.

[0011] The SPR chip storage box includes: a base, a top cover, and a silicone sleeve;

[0012] The base and the top cover are connected in an openable manner, and when they are closed, a cavity is formed inside to accommodate the SPR chip.

[0013] The silicone sleeve is fitted inside the through hole of the top cover, and the internal cavity of the silicone sleeve corresponds to the bioactive surface of the biochip.

[0014] The base is equipped with a flexible convex ring structure, which is used to support the edge of the non-bioactive surface of the biochip so that the edge of the bioactive surface of the biochip is in sealed contact with the silicone sleeve.

[0015] The internal cavity of the silicone sleeve is used to contain pure water, which is in direct contact with the bioactive surface of the biochip. The top opening of the internal cavity of the silicone sleeve is covered with a sealing film or cover to form a local humid environment, and the pure water is isolated from the adhesive strip of the SPR chip and the biochip carrier.

[0016] This utility model discloses an SPR chip storage box, which has the following beneficial effects:

[0017] First, the internal cavity of the silicone sleeve contains pure water, which comes into direct contact with the bioactive surface of the biochip. The pure water is also isolated from the adhesive strip and the biochip carrier, which solves the problems of adhesive strip detachment and separation of the biochip from the carrier caused by long-term immersion. It also prevents contaminants in the carrier or adhesive strip from coming into contact with the biochip.

[0018] Secondly, the flexible convex ring structure ensures that the edge of the bioactive surface of the biochip is in sealed contact with the silicone sleeve, effectively preventing pure water leakage.

[0019] Third, the SPR chip storage box of this utility model has a simple overall structure and is easy to operate, making it suitable for both short-term and long-term storage.

[0020] Based on the above technical solution, the following improvements can be made:

[0021] As a preferred embodiment, the flexible convex ring structure includes multiple elastic cantilever plates;

[0022] The base has an opening, and multiple elastic cantilever plates are provided on the edge of the opening towards the center, with gaps between adjacent elastic cantilever plates.

[0023] Each elastic cantilever piece has a free end that can deform elastically downwards, and the free end of the elastic cantilever piece is used to support the edge of the non-biologically active surface of the biochip.

[0024] The preferred design described above features gaps between the elastic cantilever plates, allowing each free end to deform elastically downwards independently. This enables the design to adapt to the edges of biochips of varying thicknesses, providing uniform and flexible support, preventing damage to the chip from rigid contact, and ensuring a good seal between the edge of the bioactive surface and the silicone sleeve.

[0025] As a preferred embodiment, a raised strip is provided on the upper surface of the free end of each elastic cantilever piece, the raised strip being used to abut against the edge of the non-bioactive surface of the biochip.

[0026] By adopting the above-mentioned preferred solution, the convex strip forms a line / narrow surface contact with the chip edge, ensuring precise positioning; while reducing the contact area, it ensures sufficient support force and avoids indentation on the chip surface; and with the help of elastic deformation, it achieves flexible contact, further improving the reliability of fixation.

[0027] As a preferred embodiment, the silicone sleeve includes: a cylindrical sleeve body, an annular flange extending radially from the bottom of the cylindrical sleeve body, and a sealing lip extending axially from the bottom of the cylindrical sleeve body.

[0028] The annular flange is used to engage with the through hole of the top cover, and the sealing lip is used to seal against the edge of the bioactive surface of the biochip.

[0029] Using the above-mentioned preferred solution, the annular flange is snapped into the through hole of the top cover, making the assembly firm and not easy to fall off; the sealing lip extends axially and abuts against the edge of the chip's bioactive surface to form a locally sealed humid chamber, preventing pure water from leaking out and ensuring humidity stability during long-term storage.

[0030] As a preferred option, the top cover is provided with a limiting groove for positioning the SPR chip.

[0031] Using the above-mentioned preferred solution, a pre-set limiting groove is provided on the upper cover, and the SPR chip can be quickly and initially positioned by placing it in; the limiting groove restricts the horizontal movement of the chip, reduces the risk of chip misalignment during the closing process, and facilitates batch operation.

[0032] As a preferred embodiment, a positioning post is provided in the limiting groove. The positioning post is used to pass through the positioning hole opened on the SPR chip to limit the displacement of the SPR chip in the box.

[0033] By adopting the above-mentioned preferred solution, the positioning post passes through the positioning hole on the SPR chip, which can effectively prevent the chip from sliding or rotating; and can improve the positioning accuracy, ensure that the bioactive surface and the silicone sleeve are accurately aligned, and improve the sealing and observation effect.

[0034] As a preferred option, the base has a groove at the position corresponding to the positioning post for mating with the positioning post.

[0035] With the above-mentioned preferred solution, when the top cover and the base are closed, the positioning pin is inserted into the corresponding groove of the base to form an interlock between the upper and lower parts, further restricting the vertical movement of the chip, and at the same time assisting the accurate alignment of the top cover and the base to avoid misalignment leading to sealing failure.

[0036] As a preferred embodiment, the top cover and / or base are provided with an optical docking structure, which is used to connect the SPR chip storage box to an optical device so as to observe the state of the bioactive surface of the SPR chip in real time through the silicone sleeve.

[0037] Using the above-mentioned preferred solution, the optical docking structure can connect the preservation box to a microscope or SPR imaging equipment. Light can directly irradiate the bioactive surface of the chip through the silicone sleeve and through-hole, allowing observation of the chip surface condition without opening the box. This avoids contamination or humidity changes caused by opening the box and improves the monitorability of the preservation process.

[0038] As a preferred embodiment, the top cover and the base are rotatably connected by a hinge, and / or by a magnetic connection.

[0039] The preferred scheme described above, with its hinged and / or magnetic connection, ensures convenient opening and closing and stable sealing. Attached Figure Description

[0040] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0041] Figure 1 This is a schematic diagram of the structure on the back of the SPR chip.

[0042] Figure 2 This is one of the structural schematic diagrams of the SPR chip storage box (in the open state) provided in an embodiment of this utility model.

[0043] Figure 3 for Figure 2 Enlarged view of part A in the middle.

[0044] Figure 4 The second schematic diagram of the structure of the SPR chip storage box (in the open state) provided for the embodiment of this utility model.

[0045] Figure 5 A schematic diagram of the structure of the SPR chip storage box (in the closed state) provided in the embodiment of this utility model.

[0046] Figure 6 A cross-sectional view of the SPR chip storage box (in the closed state) provided for an embodiment of this utility model.

[0047] Figure 7 for Figure 6 Enlarged view of part B in the middle.

[0048] Figure 8 A schematic diagram of the structure of the silicone sleeve provided in an embodiment of this utility model.

[0049] Among them: 1-SPR chip, 11-Biochip carrier, 12-Biochip, a-Bioactive surface, b-Non-bioactive surface, 13-Adhesive strip, 14-Positioning hole, 2-Base, 3-Top cover, 4-Silicone sleeve, c-Internal cavity, 41-Sleeve body, 42-Annular flange, 43-Sealing lip, 5-Flexible convex ring structure, 51-Elastic cantilever piece, 52-Gap, 53-Protrusion strip, 61-Limiting groove, 62-Positioning post, 63-Groove, 7-Optical docking structure. Detailed Implementation

[0050] The preferred embodiments of this utility model are described in detail below with reference to the accompanying drawings.

[0051] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0052] The expression “includes” is an “open-ended” expression, which means that there is a corresponding component or step, and should not be interpreted as excluding additional components or steps.

[0053] This embodiment discloses an SPR chip storage box for storing SPR chip 1. Figure 1 As shown, the SPR chip 1 includes: a biochip carrier 11, a biochip 12, and an adhesive strip 13. The adhesive strip 13 is used to bond and fix the edge of the biochip 12 to the back of the biochip carrier 11.

[0054] like Figures 2 to 5 As shown, the SPR chip storage box includes: a base 2, a top cover 3, and a silicone sleeve 4.

[0055] The base 2 and the top cover 3 are rotatably connected by a hinge located on one side. When the two are closed, a cavity is formed inside to accommodate the SPR chip 1.

[0056] A through hole is provided in the center of the top cover 3. The silicone sleeve 4 is an elastic hollow cylindrical structure that is fitted inside the through hole of the top cover 3. The hollow area of ​​the cylindrical silicone sleeve 4 is the internal cavity c, which is set to correspond to the bioactive surface a of the biochip 12.

[0057] The base 2 is provided with an integrally formed flexible convex ring structure 5. The flexible convex ring structure 5 is used to support the edge of the non-bioactive surface b of the biochip 12 so that the edge of the bioactive surface a of the biochip 12 is tightly fitted with the lower end surface of the silicone sleeve 4 to form a sealed contact.

[0058] In use, 100-200 microliters of pure water are injected into the internal cavity c of the silicone sleeve 4. The pure water comes into direct contact with the bioactive surface a of the biochip 12. A PE sealing film or a rigid cover is placed over the top opening of the internal cavity c of the silicone sleeve 4, thereby creating a locally closed liquid-phase humidified environment for the bioactive surface a of the biochip 12 inside the silicone sleeve 4. Since the pure water is confined within the internal cavity c of the silicone sleeve 4, while the adhesive strip 13 of the SPR chip 1 and the biochip carrier 11 are located on the outside of the silicone sleeve 4, the pure water is completely isolated from the adhesive strip 13 and the biochip carrier 11. This prevents the adhesive strip 13 from falling off due to long-term immersion and also prevents harmful substances in the biochip carrier 11 or adhesive strip 13 from migrating and contaminating the biochip 12.

[0059] The aforementioned top cover 3 and base 2 are injection molded from polypropylene (PP) material, possessing good chemical stability and mechanical strength. The aforementioned silicone sleeve 4 is made of medical-grade silicone, possessing good flexibility and water resistance.

[0060] This utility model discloses an SPR chip storage box, which has the following beneficial effects:

[0061] First, the internal cavity of the silicone sleeve 4 contains pure water, which is in direct contact with the bioactive surface a of the biochip 12. The pure water is isolated from the adhesive strip 13 and the biochip carrier 11, which solves the problem of the adhesive strip 13 falling off and the biochip 12 separating from the carrier caused by long-term soaking. It also prevents contaminants in the carrier or adhesive strip 13 from contacting the biochip 12.

[0062] Second, the flexible convex ring structure 5 ensures that the edge of the bioactive surface a of the biochip 12 is in sealed contact with the silicone sleeve 4, effectively preventing pure water leakage.

[0063] Third, the SPR chip storage box of this utility model has a simple overall structure and is easy to operate, making it suitable for both short-term and long-term storage.

[0064] Technical effectiveness verification:

[0065] When SPR chips are preserved using the SPR chip preservation box described in this invention, no adhesive layer peeling occurs within a 30-day preservation period. The surface of the SPR chips remains clean, and the signal response value attenuation is less than 5%. In contrast, approximately 60% of SPR chips preserved using the traditional centrifuge tube liquid immersion method exhibit adhesive layer peeling or surface contamination within 15 days.

[0066] The comparative experimental results above show that the SPR chip storage box of this invention can significantly improve the storage stability of SPR chips, effectively prevent adhesive strips from falling off and becoming contaminated, and extend the effective storage time of SPR chips.

[0067] In order to further optimize the implementation effect of this utility model, in some other embodiments, the remaining technical features are the same, except that the flexible convex ring structure 5 includes four elastic cantilever plates 51.

[0068] The base 2 has an opening, and four elastic cantilever plates 51 are provided on the edge of the opening towards the center. There is a gap 52 between adjacent elastic cantilever plates 51.

[0069] Each elastic cantilever piece 51 has a free end that can undergo elastic deformation downwards under external force, and the upper surface of the free end of the elastic cantilever piece 51 is used to support the edge of the non-biologically active surface b of the biochip 12.

[0070] like Figures 6 to 7 As shown, the biochip 12 is bonded and fixed to the square hole area of ​​the biochip carrier 11 by an adhesive strip 13. The size of the biochip 12 is set to completely cover the square hole, and the adhesive strip 13 is attached to the back of the biochip carrier 11 and located on the outer periphery of the square hole area. The bioactive surface a of the biochip 12 faces the front of the biochip carrier 11 and can directly contact the pure water in the internal cavity c of the silicone sleeve 4; the non-bioactive surface b of the biochip 12 faces the back of the biochip carrier 11.

[0071] Specifically, when the SPR chip 1 is placed inside the SPR chip storage box and the top cover 3 is closed with the base 2, the bioactive surface a of the biochip 12 faces upwards, and the non-bioactive surface b of the biochip 12 faces downwards. At this time, both the flexible convex ring structure 5 and the silicone sleeve 4 on the base 2 undergo elastic deformation. The edge of the biochip 12 presses the elastic cantilever plate 51 downwards, and the rebound force generated by the elastic cantilever plate 51 pushes the biochip 12 upwards, making the edge of its bioactive surface a in close contact with the silicone sleeve 4. The flexible convex ring structure 5 is integrally injection molded from polypropylene (PP) material, and its rigidity is greater than that of the silicone sleeve 4; at the same time, the silicone sleeve 4 undergoes adaptive compression deformation under pressure. Due to the relatively high rigidity of the flexible convex ring structure 5, it can provide a stable reverse support force for the silicone sleeve 4 after deformation, so that the edge of the bioactive surface a of the biochip 12 is tightly fitted with the lower end face of the silicone sleeve 4, forming a sealed contact and preventing pure water leakage.

[0072] In another specific embodiment, based on the above embodiment, a protrusion 53 is provided on the upper surface of the free end of each elastic cantilever piece 51. The protrusion 53 is provided along the chip edge extension direction, and the upper end surface of the protrusion 53 is used to abut against the edge of the non-biologically active surface b of the biochip 12.

[0073] The protrusion 53 forms a line / narrow surface contact with the chip edge, ensuring precise positioning; it reduces the contact area while ensuring sufficient support, avoiding indentations on the chip surface; and it achieves flexible contact through elastic deformation, further improving the reliability of fixation.

[0074] In another specific implementation, based on the above embodiments, such as Figure 8 As shown, the silicone sleeve 4 is a one-piece molded medical-grade silicone component, including: a cylindrical sleeve body 41, an annular flange 42 extending radially from the bottom of the cylindrical sleeve body 41, and a sealing lip 43 extending axially from the bottom of the cylindrical sleeve body 41.

[0075] The outer diameter of the annular flange 42 is larger than the diameter of the through hole in the upper cover 3, and it is used to snap onto the upper edge of the through hole in the upper cover 3 to prevent the silicone sleeve 4 from falling out of the through hole. The inner diameter of the sealing lip 43 is slightly smaller than the outer diameter of the bioactive surface a of the biochip 12. When the biochip 12 is pushed upward by the flexible convex ring structure 5, the sealing lip 43 undergoes elastic deformation and fits tightly against the edge of the bioactive surface a, forming a sealed contact. This structure further effectively prevents pure water in the internal cavity of the silicone sleeve 4 from leaking out, ensuring humidity stability during long-term storage.

[0076] In order to further optimize the implementation effect of this utility model, in some other embodiments, the remaining technical features are the same, except that the lower surface of the upper cover 3 (i.e. the side facing the base 2) is provided with a limiting groove 61 for positioning the SPR chip 1.

[0077] When placing the SPR chip, the SPR chip is placed directly into the limiting groove 61. The groove wall can restrict the movement of the SPR chip in the horizontal plane and prevent the SPR chip from shifting during the closing process.

[0078] In another specific embodiment, based on the above embodiment, the limiting groove 61 is provided with two positioning posts 62. The positioning posts 62 extend upward perpendicularly to the plane of the upper cover 3, and their positions correspond to the positioning holes 14 pre-drilled on the SPR chip 1. When the SPR chip 1 is placed into the limiting groove 61, the positioning posts 62 pass through the positioning holes 14, thereby precisely limiting the rotational and translational degrees of freedom of the SPR chip in the plane. The positioning posts 62, in conjunction with the limiting groove 61, can significantly improve the positioning accuracy of the SPR chip and ensure that the bioactive surface is accurately aligned with the sealing lip 43 of the silicone sleeve 4.

[0079] In another specific embodiment, based on the above embodiment, a groove 63 is provided on the base 2 at the position corresponding to the positioning post 62. The shape of the groove 63 is adapted to the end of the positioning post 62. When the upper cover 3 is closed with the base 2, the positioning post 62 extends into the groove 63 to form a fit, which not only avoids squeezing the chip, but also helps the upper and lower covers to be accurately aligned.

[0080] To further optimize the implementation effect of this utility model, in some other embodiments, the remaining technical features are the same, except that an optical docking structure 7 is provided at the center of the upper surface of the top cover 3. The optical docking structure 7 may be, but is not limited to, a C-type threaded interface. Through this C-type threaded interface, the SPR chip preservation box can be directly screwed onto the observation port of an optical device (such as a microscope or SPR imaging equipment). The light beam emitted by the optical device passes through the silicone sleeve 4 and the through hole of the top cover 3, directly irradiating the bioactive surface of the biochip 12, thereby allowing real-time observation of the preservation status of the chip surface without opening the preservation box or disrupting the internal humid environment. This structure significantly improves the monitorability of the preservation process.

[0081] To further optimize the implementation effect of this utility model, in some other embodiments, the remaining technical features are the same, except that the upper cover 3 and the base 2 are rotatably connected by a hinge on one side, allowing the upper cover 3 to be flipped open relative to the base 2. On the opposite edge, permanent magnets or ferromagnetic metal sheets of opposite polarity are respectively embedded in the upper cover 3 and the base 2. When the upper cover 3 is flipped onto the base 2, the hinge side provides rotational positioning, and the magnetic side automatically attracts and locks in place by magnetic force, thereby firmly fixing the upper cover 3 and the base 2.

[0082] In the description of this utility model, it should be understood that the terms "coaxial", "bottom", "one end", "top", "middle", "other end", "upper", "side", "top", "inner", "front", "center", "both ends", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0083] In this utility model, unless otherwise explicitly specified and limited, the terms "installation", "setting", "connection", "fixing", "screw connection", etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal connection of two components or the interaction between two components. Unless otherwise explicitly limited, those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

Claims

1. A SPR chip storage case for storing a SPR chip, the SPR chip comprising: A biochip carrier, a biochip, and an adhesive strip, wherein the adhesive strip is used to bond and fix the edge of the biochip to the biochip carrier; Its features include: a base, a top cover, and a silicone sleeve; The base and the top cover are detachably connected, and when they are closed, they form a cavity inside to accommodate the SPR chip. The silicone sleeve is fitted inside the through hole of the upper cover, and the internal cavity of the silicone sleeve corresponds to the bioactive surface of the biochip. The base is provided with a flexible convex ring structure, which is used to support the edge of the non-bioactive surface of the biochip so that the edge of the bioactive surface of the biochip is in sealed contact with the silicone sleeve. The internal cavity of the silicone sleeve is used to contain pure water, which is in direct contact with the bioactive surface of the biochip. The top opening of the internal cavity of the silicone sleeve is covered with a sealing film or cover to form a localized humid environment, and the pure water is isolated from the adhesive strip of the SPR chip and the biochip carrier.

2. The SPR chip storage case according to claim 1, characterized by The flexible convex ring structure includes multiple elastic cantilever plates; The base is provided with an opening, and the multiple elastic cantilever plates are provided with the edge of the opening extending towards the center, with gaps between adjacent elastic cantilever plates. Each of the elastic cantilever pieces has a free end that can elastically deform downwards, and the free end of the elastic cantilever piece is used to support the edge of the non-bioactive surface of the biochip.

3. The SPR chip storage case according to claim 2, characterized by Each of the elastic cantilever plates has a raised strip on its upper surface at the free end, the raised strip being used to abut against the edge of the non-bioactive surface of the biochip.

4. The SPR chip storage case according to claim 1, characterized by The silicone sleeve includes: a cylindrical sleeve body, an annular flange extending radially from the bottom of the cylindrical sleeve body, and a sealing lip extending axially from the bottom of the cylindrical sleeve body. The annular flange is used to engage with the through hole of the upper cover, and the sealing lip is used to seal against the edge of the bioactive surface of the biochip.

5. The SPR chip storage box according to claim 1, characterized in that, The upper cover is provided with a limiting groove for positioning the SPR chip.

6. The SPR chip storage box according to claim 5, characterized in that, The limiting groove is provided with a positioning post, which is used to pass through the positioning hole opened on the SPR chip to limit the displacement of the SPR chip in the box.

7. The SPR chip storage box according to claim 6, characterized in that, The base has a groove corresponding to the position of the positioning post, for engaging with the positioning post.

8. The SPR chip storage box according to claim 1, characterized in that, The top cover and / or the base are provided with an optical docking structure, which is used to connect the SPR chip storage box to an optical device so as to observe the state of the bioactive surface of the SPR chip in real time through the silicone sleeve.

9. The SPR chip storage box according to claim 1, characterized in that, The upper cover and the base are rotatably connected by a hinge and / or by a magnetic attraction.

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