A stem cell culture device

Abstract

This invention relates to the field of cell culture technology and provides a stem cell culture device, including a culture box. The culture box has several equidistant grooves in its inner cavity, and drawers are slidably mounted inside the grooves. Limiting grooves are symmetrically provided on both sides of the drawers. A handle is fixedly mounted on the outer surface of the drawers, and several square holes are provided on the inner surface of the drawers. A limiting component is symmetrically provided at one end of the outer wall of the culture box. The limiting component includes a mounting block, which is symmetrically fixed to the outer wall of the culture box. The inner cavity of the mounting block has a moving cavity. By providing the limiting component, when the culture dish is placed in the square hole in the drawer and the drawer is pushed into the culture box, releasing the push-pull rod causes a reset spring to move the moving plate and limiting block outwards, allowing the limiting block to insert into the limiting groove and limit the drawer. This prevents the culture box from sliding out when impacted, improving the stability and practicality of the device.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a stem cell culture device. Background Technology

[0002] Stem cells are ideal target cells for gene therapy, creating an environment for the stable expression of target genes. By utilizing hematopoietic stem cells and mesenchymal stem cells, the body's hematopoietic and immune systems can be rebuilt. This can be used as a routine treatment for blood diseases such as leukemia and aplastic anemia, as well as diseases with immune system deficiency and hyperactivity. Therefore, stem cells need to be cultured. However, existing stem cell culture equipment is not convenient for temperature control during culture, resulting in a low survival rate of cultured stem cells.

[0003] For example, CN222455051U discloses a stem cell culture device, including a box, a chute, a culture drawer, a rod, and a heat insulation plate. Four heat insulation plates are fixedly installed in a vertical array on the inner side of the box. A culture chamber is opened at the top of the inner side of the box and between two adjacent heat insulation plates. The inner side wall of the culture chamber is provided with a chute. The chute of two adjacent culture chambers is perpendicular to each other. Slides are fixedly installed on the left and right side walls of the culture drawer. A handle is fixedly installed in the center of the outer side wall of the culture drawer. The slides slide horizontally on the inner side of the chute. Several fixing holes are evenly opened on the inner bottom of the culture drawer. A ventilation chamber is opened on the inner bottom of the box. A ventilation pipe is connected to the side wall of the culture chamber. The other end of the ventilation pipe is connected to the ventilation chamber. This application improves the culture efficiency of the device by setting up a separate culture drawer for taking and putting in stem cells, so as not to delay other stem cell cultures.

[0004] However, in existing technologies, culture devices typically store petri dishes in drawers. Currently, traditional culture devices do not have drawer restraints, which can cause the drawer to slide out when the culture device is bumped, affecting the stability of the petri dishes and thus limiting the practicality of the device to some extent. Utility Model Content

[0005] The purpose of this invention is to solve the problem that in the existing technology, the drawers of traditional culture devices are not limited, which causes the drawers to slide out when the culture device is collided, affecting the stability of the culture dishes.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a stem cell culture device, comprising a culture box, wherein a plurality of sliding grooves are equidistantly provided in the inner cavity of the culture box, a drawer is slidably arranged inside the sliding grooves, limiting grooves are symmetrically provided on both sides of the drawer, a handle is fixedly provided on the outer surface of the drawer, a plurality of square holes are provided on the inner surface of the drawer, a limiting component is symmetrically provided at one end of the outer wall of the culture box, the limiting component includes a mounting block, the mounting block is symmetrically fixedly provided on the outer wall of the culture box, a moving cavity is provided in the inner cavity of the mounting block, a moving plate is slidably arranged inside the moving cavity, a plurality of return springs are elastically provided between the outer surface of the moving plate and the inner wall of the moving cavity, a push-pull rod is fixedly provided at the center of the outer surface of the moving plate, and one end of the push-pull rod is located outside the mounting block, a plurality of limiting blocks are equidistantly fixedly provided on the inner side of the moving plate, and the limiting blocks are inserted and connected to the limiting grooves.

[0007] In a preferred embodiment, a plurality of air inlet pipes are fixedly arranged at equal intervals on one side of the outer surface of the incubator, and a first one-way solenoid valve is provided on the surface of the air inlet pipes.

[0008] In a preferred embodiment, several exhaust pipes are fixedly arranged at equal intervals on the other side of the outer surface of the incubator, and a second one-way solenoid valve is provided on the surface of the exhaust pipes.

[0009] In a preferred embodiment, heating tubes are fixedly installed above the internal mounting cavity of the incubator.

[0010] In a preferred embodiment, a temperature sensor is fixedly installed on one side of the internal mounting cavity of the incubator, and a humidity sensor is fixedly installed on the other side of the internal mounting cavity of the incubator.

[0011] In a preferred embodiment, a PLC control terminal is fixedly installed on the upper surface of the incubator, and shock-absorbing supports are fixedly installed at the four corners of the lower surface of the incubator.

[0012] In a preferred embodiment, the PLC control terminal is connected to the heating element, temperature sensor, humidity sensor, first one-way solenoid valve, and exhaust pipe via signal transmission.

[0013] Compared with the prior art, the advantages and positive effects of this utility model are as follows:

[0014] This invention features a limiting component. When the petri dish is placed in the square hole in the drawer and the drawer is pushed into the incubator, releasing the push-pull rod causes the reset spring to move the moving plate and the limiting block outwards. This allows the limiting block to insert into the limiting groove and limit the drawer, preventing the incubator from sliding out when it is hit by a collision. This improves the stability and practicality of the device.

[0015] This invention utilizes a combination of a heating element, a temperature sensor, a humidity sensor, and an air intake / exhaust pipe. The temperature sensor monitors the temperature of the petri dishes inside the incubator in real time to control the heating level of the heating element, while the humidity sensor monitors the air humidity inside the petri dishes to control the start and stop of the air intake / exhaust pipe, thereby adjusting the air humidity inside the incubator. This enhances the accuracy of environmental monitoring and further improves the practicality of the device. Attached Figure Description

[0016] Figure 1 A three-dimensional structural diagram of a stem cell culture device provided by this utility model;

[0017] Figure 2 A three-dimensional cross-sectional view of a stem cell culture device provided by this utility model;

[0018] Figure 3 This utility model provides a stem cell culture device. Figure 2 Enlarged schematic diagram of the structure at point A in the middle;

[0019] Figure 4 A cross-sectional view of the culture box structure of a stem cell culture device provided by this utility model;

[0020] Figure 5 A schematic diagram of the square hole structure of a stem cell culture device provided by this utility model.

[0021] Legend:

[0022] 1. Incubator; 2. Vibration damping support; 3. PLC control terminal; 4. Air inlet pipe; 5. First one-way solenoid valve; 6. Exhaust pipe; 7. Second one-way solenoid valve; 8. Heating element; 9. Temperature sensor; 10. Humidity sensor; 11. Slide rail; 12. Drawer; 13. Square hole; 14. Handle; 15. Limiting groove; 16. Mounting block; 17. Moving cavity; 18. Moving plate; 19. Limiting block; 20. Push-pull rod; 21. Return spring. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Please see Figure 1-5This utility model provides a technical solution: a stem cell culture device, including a culture box 1. The inner cavity of the culture box 1 has several equidistant sliding grooves 11. A drawer 12 is slidably arranged inside the sliding grooves 11. Limiting grooves 15 are symmetrically arranged on both sides of the drawer 12. A handle 14 is fixedly arranged on the outer surface of the drawer 12. Several square holes 13 are opened on the inner surface of the drawer 12. A limiting component is symmetrically arranged at one end of the outer wall of the culture box 1. The limiting component includes a mounting block 16, which is symmetrically fixed to the outer wall of the culture box 1. A movable cavity 17 is opened inside the mounting block 16. A movable plate 18 is slidably arranged inside the movable cavity 17. The outer surface of the movable plate 18 is flush with the movable cavity 17. Several return springs 21 are elastically arranged between the inner sidewalls of the moving plate 18. A push-pull rod 20 is fixedly arranged at the center of the outer surface of the moving plate 18, and one end of the push-pull rod 20 is located outside the mounting block 16. Several limiting blocks 19 are fixedly arranged at equal intervals on the inner side of the moving plate 18, and the limiting blocks 19 are inserted into the limiting groove 15. When the petri dish is placed into the square hole 13 in the drawer 12 and the drawer 12 is pushed into the incubator 1, the push-pull rod 20 is released, the return springs 21 reset and drive the moving plate 18 and the limiting blocks 19 to move outward, so that the limiting blocks 19 are inserted into the limiting groove 15 to limit the drawer 12, so as to prevent the incubator 1 from sliding out when it is hit, thus ensuring the stability of the device.

[0025] like Figure 1-5 As shown, several air inlet pipes 4 are fixedly arranged at equal intervals on one side of the outer surface of the incubator 1. A first one-way solenoid valve 5 is provided on the surface of the air inlet pipe 4. Several exhaust pipes 6 are fixedly arranged at equal intervals on the other side of the outer surface of the incubator 1. A second one-way solenoid valve 7 is provided on the surface of the exhaust pipe 6. The first one-way solenoid valve 5 controls the air inlet pipe 4 to take in air, and the second one-way solenoid valve 7 controls the exhaust pipe 6 to take out air, so as to control the change of air humidity inside the incubator 1.

[0026] like Figure 1-5 As shown, heating tubes 8 are fixedly installed above the internal mounting cavity of the incubator 1. A temperature sensor 9 is fixedly installed on one side of the internal mounting cavity of the incubator 1, and a humidity sensor 10 is fixedly installed on the other side of the internal mounting cavity of the incubator 1. A PLC control terminal 3 is fixedly installed on the upper surface of the incubator 1, and shock-absorbing supports 2 are fixedly installed at the four corners of the lower surface of the incubator 1. The PLC control terminal 3 is connected to the heating tubes 8, temperature sensor 9, humidity sensor 10, first one-way solenoid valve 5, and exhaust pipe 6 via signal transmission. The temperature sensor 9 monitors the temperature of the petri dish inside the incubator 1 in real time to control the heating degree of the heating tubes 8, while the humidity sensor 10 monitors the air humidity of the petri dish inside the incubator 1 to control the start and stop of the inlet and outlet pipes to adjust the air humidity inside the incubator 1, thereby enhancing the accuracy of environmental monitoring.

[0027] Working principle: In use, first insert the petri dish into the square hole 13, then pull the push-pull rod 20 outward. The push-pull rod 20 moves the moving plate 18 and the limiting block 19 outward and compresses the return spring 21. Then, grasp the handle 14 and slide the drawer 12 along the slide groove 11 into the inner cavity of the incubator 1. Release the push-pull rod 20, and the return spring 21 returns to its original position, moving the moving plate 18 and the limiting block 19 outward. This causes the limiting block 19 to insert into the limiting groove 15, limiting the drawer 12 and preventing the incubator 1 from being stopped. When impacted, the incubator 1 will slide out, and with the cooperation of the shock-absorbing support 2, a certain degree of shock absorption is achieved, ensuring the stability of the culture dish placement. The temperature sensor 9 monitors the temperature of the culture dish inside the incubator 1 in real time to control the heating degree of the heating tube 8, while the humidity sensor 10 monitors the air humidity inside the culture dish of the incubator 1 to control the start and stop of the air inlet and outlet pipes to adjust the air humidity inside the incubator 1. This enhances the accuracy of environmental monitoring, improves the culture effect of stem cells, and is suitable for large-scale promotion.

[0028] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model in any other way. Any person skilled in the art may make changes or equivalent variations to the above-disclosed technical content and apply them to other fields. However, any simple modifications, equivalent variations and alterations made to the above embodiments based on the technical essence of the present utility model without departing from the technical solution of the present utility model shall still fall within the protection scope of the technical solution of the present utility model.

Claims

1. A stem cell culture device, comprising a culture box (1), characterized in that: The incubator (1) has several equally spaced sliding grooves (11) in its inner cavity. A drawer (12) is slidably mounted inside each groove (11). Limiting grooves (15) are symmetrically arranged on both sides of the drawer (12). A handle (14) is fixedly mounted on the outer surface of the drawer (12). Several square holes (13) are opened on the inner surface of the drawer (12). A limiting component is symmetrically arranged at one end of the outer wall of the incubator (1). The limiting component includes a mounting block (16), which is symmetrically fixed to the outer wall of the incubator (1). The inner cavity of the mounting block (16) is provided with a movable cavity (17). A movable plate (18) is slidably arranged inside the movable cavity (17). Several return springs (21) are elastically arranged between the outer surface of the movable plate (18) and the inner wall of the movable cavity (17). A push-pull rod (20) is fixedly arranged at the center of the outer surface of the movable plate (18), and one end of the push-pull rod (20) is located outside the mounting block (16). Several limiting blocks (19) are fixedly arranged at equal intervals on the inner side of the movable plate (18), and the limiting blocks (19) are inserted and connected to the limiting groove (15).

2. The stem cell culture device according to claim 1, characterized in that: A plurality of air inlet pipes (4) are fixedly arranged at equal intervals on one side of the outer surface of the incubator (1), and a first one-way solenoid valve (5) is provided on the surface of the air inlet pipes (4).

3. The stem cell culture device according to claim 1, characterized in that: Several exhaust pipes (6) are fixedly arranged at equal intervals on the other side of the outer surface of the incubator (1), and a second one-way solenoid valve (7) is provided on the surface of the exhaust pipes (6).

4. The stem cell culture device according to claim 1, characterized in that: Heating tubes (8) are fixedly installed above the internal mounting cavity of the incubator (1).

5. The stem cell culture device according to claim 1, characterized in that: A temperature sensor (9) is fixedly installed on one side of the internal mounting cavity of the incubator (1), and a humidity sensor (10) is fixedly installed on the other side of the internal mounting cavity of the incubator (1).

6. The stem cell culture device according to claim 1, characterized in that: The upper surface of the incubator (1) is fixedly equipped with a PLC control terminal (3), and the four corners of the lower surface of the incubator (1) are fixedly equipped with shock-absorbing supports (2).

7. A stem cell culture device according to claim 6, characterized in that: The PLC control terminal (3) is connected to the heating tube (8), temperature sensor (9), humidity sensor (10), first one-way solenoid valve (5) and exhaust pipe (6) by signal transmission.

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