A culture device and culture system

Abstract

This application discloses a culture device and culture system, relating to the technical field of organoids. The culture device includes: a first base, comprising a bracket, a first upper support platform, a first support frame, and a first lower support platform, the first upper support platform and the first lower support platform being arranged opposite each other along a third direction, the first support frame being connected to the first upper support platform and the first lower support platform, and the bracket being disposed on the first lower support platform and having a support portion for supporting a culture box; a gas guiding assembly, having a communicating first gas port and a second gas port, and located between the first upper support platform and the bracket, along a third direction, the gas guiding assembly and the bracket being opposite each other; and a first drive mechanism, mounted on the first upper support platform, for driving the gas guiding assembly to move along a third direction, so that the second gas port of the gas guiding assembly is connected or disconnected from the gas passage interface of the culture box.

Description

Technical Field

[0001] This application relates to the technical field of organoids, and more particularly to a culture device and culture system. Background Technology

[0002] Traditional preclinical models (including two-dimensional cell models, three-dimensional spherical cells, and various experimental animal models) have limitations in realistically simulating human physiological structures and functions, leading to significant challenges in predicting drug responses. Organ-on-a-chip technology can differentiate cells into organoid-specific cell types, highly mimicking the development of human organs in vitro and reconstructing human organ structures to replace animal models, thus providing more possibilities for research on human diseases.

[0003] Currently, organ-on-a-chip culture requires a specific carbon dioxide incubator to provide the necessary growth conditions (such as suitable carbon dioxide concentration, temperature, and humidity). During organ-on-a-chip culture, microfluidic pressure technology is a crucial element in constructing physiologically relevant models. By precisely controlling parameters such as pressure and flow rate, the complex microenvironment of organs in vivo can be simulated.

[0004] In related technologies, organ-on-a-chip is placed in a culture box for culture, but the connection between the pressure supply device and the culture box is inconvenient during culture. Utility Model Content

[0005] Based on this, this application provides a culture device and culture system to solve the problem of inconvenient connection between pressure supply device and culture box in related technologies.

[0006] In a first aspect, embodiments of this application provide a culture apparatus, comprising:

[0007] The first base includes a bracket, a first upper support platform, a first support frame, and a first lower support platform. The first upper support platform and the first lower support platform are arranged opposite each other along a third direction. The first support frame is connected to the first upper support platform and the first lower support platform. The bracket is disposed on the first lower support platform and has a support portion for supporting the culture box.

[0008] An air guiding assembly has a first air port and a second air port that are connected, and is located between the first upper support platform and the bracket. Along the third direction, the air guiding assembly and the bracket are opposite to each other.

[0009] A first driving mechanism, mounted on the first upper support platform, is used to drive the gas guiding assembly to move along the third direction, so that the second air port of the gas guiding assembly is connected or disconnected from the gas passage interface of the culture box.

[0010] In some embodiments, the first base further includes:

[0011] A guide rod is provided along the third direction and connected to the first support frame, and the air guiding assembly is slidably connected to the guide rod along the third direction.

[0012] In some embodiments, the first drive mechanism includes:

[0013] A reset component, sleeved outside the guide rod, acts on the air guide assembly and the first support frame, applying a force to the air guide assembly to move it away from the bracket;

[0014] A drive assembly is installed on the first upper support platform. The output end of the drive assembly acts on the air guide assembly to drive the air guide assembly to move toward the bracket so that the second air port is connected to the air passage interface.

[0015] In some embodiments, the driving component includes:

[0016] Support, installed on the first upper bearing platform;

[0017] The drive component is installed on the support;

[0018] The oscillating assembly has a first end and a second end, the first end being connected to the output shaft of the driving member;

[0019] An output component is slidably connected to the support along the third direction, and the output component is located between the swing assembly and the air guiding assembly. The second end of the swing assembly acts on the output component, and the output component acts on the air guiding assembly.

[0020] In some embodiments, the support is disposed above the first upper support platform, the first upper support platform is provided with a clearance through hole, and the output component is located in the clearance through hole.

[0021] In some embodiments, the air guiding assembly includes:

[0022] An air guide has a first air port and a second air port, and the air guide and the bracket are arranged opposite to each other along the third direction;

[0023] A connector is attached to the air guide member. The connector has a sliding hole arranged along the third direction. The connector is slidably sleeved on the outside of the guide rod through the sliding hole.

[0024] In some embodiments, along the third direction, both the guide rod and the connector are located below the first upper support platform.

[0025] In some embodiments, the air guiding assembly is provided with two connectors, which are respectively disposed on both sides of the air guiding assembly along a first direction and located on the side of the air guiding assembly closer to the first upper support platform, wherein the first direction is perpendicular to the third direction.

[0026] In some embodiments, the air guiding assembly further includes:

[0027] A force-bearing component is connected to the air guide component and is located along the third direction. The force-bearing component is disposed on the side of the air guide component near the first upper support platform and between the two connecting components. The output end of the drive assembly acts on the force-bearing component.

[0028] In some embodiments, the bracket has a plurality of support portions spaced apart along a first direction, the first lower support platform has a first groove arranged along the first direction, the bracket is slidably disposed in the first groove along the first direction, and the first direction is perpendicular to the third direction.

[0029] In some embodiments, the bracket is provided with a handle portion, which is located outside the first slide groove.

[0030] In some embodiments, the bottom wall of the first chute has a third observation port, the bracket has a fourth observation port, and the third and fourth observation ports are opposite each other along the third direction; the observation window of the culture box is exposed to the outside through the third and fourth observation ports.

[0031] In some embodiments, the first air port and the second air port are connected through a flow channel, and the air guiding assembly further has a mounting port connected to the flow channel, the mounting port being located between the first air port and the second air port;

[0032] The culture device also includes a solenoid valve, which is installed in the mounting port and used to control the opening and closing of the flow channel.

[0033] In some embodiments, the first base includes two brackets spaced apart along a second direction, and the culture device includes two gas guiding components spaced apart along the second direction. The gas guiding components and the brackets are arranged in a one-to-one correspondence, and the second direction is perpendicular to the third direction.

[0034] Secondly, embodiments of this application provide a culture system, including the culture apparatus described in the first aspect.

[0035] This application has at least the following beneficial effects:

[0036] The culture device uses a first drive mechanism to move the gas delivery assembly, allowing the gas inlet of the culture box to connect or disconnect with the second gas port of the gas delivery assembly. This design eliminates the need for manual connection or disconnection of the culture box and the pressure supply device, making connection and disconnection more convenient and reducing the risk of organ-on-a-chip contamination within the culture box. The culture device also uses a first upper support platform to shield against dust, reducing the likelihood of dust adhering to the surface of the culture box or gas delivery assembly, ensuring the cleanliness of the gas delivery assembly and the culture box, and helping to maintain suitable culture conditions within the culture box. Attached Figure Description

[0037] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0038] Figure 1 This is a schematic diagram of the structure of the culture device after a culture box is set on it in one or more embodiments of this application.

[0039] Figure 2 This is a schematic diagram of the structure of the culture device in one or more embodiments of this application.

[0040] Figure 3 This is an exploded schematic diagram of the culture device in one or more embodiments of this application.

[0041] Figure 4 This is a schematic diagram of the structure of the culture box in one or more embodiments of this application. Figure 1 .

[0042] Figure 5 This is a schematic diagram of the structure of the culture box in one or more embodiments of this application. Figure 2 .

[0043] Figure 6 This is a schematic diagram of the gas guiding component of the culture device in one or more embodiments of this application.

[0044] Figure 7 This is a schematic diagram of the structure of the driving component of the culture device in one or more embodiments of this application.

[0045] Figure 8 This is an exploded view of the driving components of the culture apparatus in one or more embodiments of this application. Figure 1 .

[0046] Figure 9 This is an exploded view of the driving components of the culture apparatus in one or more embodiments of this application. Figure 2 .

[0047] Figure 10 This is a schematic diagram of the structure of the culture system in one or more embodiments of this application. Figure 1 .

[0048] Figure 11 This is a schematic diagram of the structure of the culture system in one or more embodiments of this application. Figure 2 .

[0049] Figure 12 This is a schematic diagram of the imaging device in one or more embodiments of this application.

[0050] Figure 13 This is an exploded schematic diagram of the imaging device in one or more embodiments of this application.

[0051] Explanation of reference numerals in the attached figures:

[0052] 2000-Cultural box, 2000a-Gas interface, 2000b-Observation window, 1000-Cultural system, 100-Cultural device, 110-First base, 110a-Bearing part, 110b-First observation port, 120-Support frame, 121-Frame body, 121a-First slide groove, 121b-Third observation port, 1211-First upper bearing platform, 1211a-Avoidance through hole, 12 12-First support frame, 1213-First lower bearing platform, 122-Bracket, 1221-Handle, 122a-Fourth observation port, 130-Guide rod, 140-Air guiding assembly, 140a-First air port, 140b-Second air port, 140c-Mounting port, 141-Air guiding component, 142-Connector, 142a-Sliding hole, 143-Force-bearing component, 1431-Force-bearing part, 143 2-Force transmission unit, 150-First drive mechanism, 160-Reset component, 170-Drive assembly, 171-Driving component, 1711-Output shaft, 172-Swing assembly, 1721-Swing arm, 1722-Rotating wheel, 173-Output component, 174-Support, 180-Solenoid valve, 200-Imaging device, 210-Second base, 210a-Second observation port, 210b-Second slide, 211-Second lower support platform, 212-Second upper support platform, 213-Support column, 220-Imaging module, 230-Second drive mechanism, 240-First drive assembly, 241-First support platform, 242-First drive component, 250-Second drive assembly, 251-Second support platform, 252-Second drive component, 260-Third drive assembly, 261-Third drive component, 262-Sliding seat. Detailed Implementation

[0053] To make the objectives, technical solutions, and advantages of this application clearer, the technical solutions in the embodiments of this application will be described in more detail below with reference to the accompanying drawings. In the drawings, the same or similar reference numerals denote the same or similar components or components having the same or similar functions throughout. The described embodiments are some, but not all, of the embodiments of this application. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain this application, and should not be construed as limiting this application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without creative effort are within the scope of protection of this application. The embodiments of this application will be described in detail below with reference to the accompanying drawings.

[0054] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, an indirect connection through an intermediate medium, or the internal communication between two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0055] In the description of this application, it should be understood that the terms "upper", "lower", "front", "back", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0056] The terms “first,” “second,” and “third” (if any) in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence.

[0057] Furthermore, the terms “comprising” and “having”, and any variations thereof, are intended to cover non-exclusive inclusion, such that a process, method, system, product, or display that includes a series of steps or units is not necessarily limited to those steps or units that are explicitly listed, but may include other steps or units that are not explicitly listed or that are inherent to such process, method, product, or display.

[0058] In related technologies, organ-on-a-chip is placed in a culture box for culture. During culture, the pressure supply device and the culture box need to be manually connected, which is complicated to operate, inconvenient to connect, and increases the risk of contamination of the organ-on-a-chip in the culture box.

[0059] In view of this, the inventors designed a culture device 100 and a culture system 1000. The culture device 100 drives the gas guiding component 140 to move through the first driving mechanism 150, so that the gas interface 2000a of the culture box 2000 is connected or disconnected from the second gas port 140b of the gas guiding component 140. With this design, it is not necessary to manually connect or disconnect the culture box 2000 and the pressure supply device. Connecting or disconnecting the culture box 2000 and the pressure supply device is more convenient, and the risk of organ-on-a-chip contamination in the culture box 2000 is also reduced.

[0060] The culture apparatus 100 and culture system 1000 provided in the embodiments of this application are described in detail below with reference to the accompanying drawings.

[0061] In the attached diagram, X represents the first direction, Y represents the second direction, and Z represents the third direction. The first, second, and third directions are perpendicular to each other.

[0062] like Figure 1 , Figure 2 and Figure 3 As shown, the culture device 100 includes a first base 110, an air guiding assembly 140, and a first drive mechanism 150. The first base 110 includes a frame 121 and a bracket 122. The frame 121 includes a first upper support platform 1211, a first support frame 1212, and a first lower support platform 1213. The first upper support platform 1211 and the first lower support platform 1213 are arranged opposite each other along a third direction. The first support frame 1212 is connected to the first upper support platform 1211 and the first lower support platform 1213. The bracket 122 is disposed on the first lower support platform 1213 and has a support portion 110a for supporting the culture box 2000. The air guiding assembly 140 has a communicating first air port 140a and a second air port 140b, and is located between the first upper support platform 1211 and the bracket 122. Along a third direction, the air guiding assembly 140 and the bracket 122 are opposite each other. The first drive mechanism 150 is installed on the first upper support platform 1211 and is used to drive the gas guide assembly 140 to move in a third direction so that the second air port 140b of the gas guide assembly 140 is connected or disconnected from the gas passage interface 2000a of the culture box 2000.

[0063] The first upper support platform 1211 is located above the first lower support platform 1213. The lower side of the first support frame 1212 is connected to the first lower support platform 1213, and the upper side is connected to the first upper support platform 1211. The first upper support platform 1211 is supported by the first support frame 1212, and the first support frame 1212 is supported by the first lower support platform 1213. The bracket 122 is disposed on the first lower support platform 1213, located between the first lower support platform 1213 and the first upper support platform 1211, and is supported by the first lower support platform 1213. The first drive mechanism 150 is mounted on the first upper support platform 1211 and is supported by the first upper support platform 1211.

[0064] The support portion 110a on the bracket 122 is used to support the culture box 2000. The gas delivery assembly 140 is located above the bracket 122. When culturing organ-on-a-chip, the culture box 2000 is placed on the support portion 110a and between the support portion 110a and the gas delivery assembly 140.

[0065] The culture box 2000 is used to culture organ-on-a-chip. The structure of the culture box 2000 is diverse and is known to those skilled in the art, so it will not be described in detail here.

[0066] like Figure 4 As shown, the culture chamber 2000 typically has multiple gas ports 2000a, which need to be connected to the gas ports of a pressure supply device. The pressure supply device provides pressure to the organ-on-a-chip within the culture chamber 2000. Some gas ports of the pressure supply device output positive pressure, while others output negative pressure. Some gas ports 2000a are connected to the negative pressure ports of the pressure supply device. Negative pressure can be used to simulate the inhalation and exhalation processes of the lungs. For example, in a lung-on-a-chip, negative pressure can cause changes in the volume of the air chamber side to simulate respiratory movements. Negative pressure can also be used to precisely control the direction and speed of fluid flow, such as using negative pressure to attract culture medium or cell suspension in a microfluidic chip. Some gas ports 2000a are connected to the positive pressure ports of the pressure supply device. Positive pressure can be used to drive the flow of culture medium or gas in microfluidic channels, ensuring the uniform distribution of nutrients and gases. Positive pressure can also be used to simulate blood flow or respiratory movements, such as using positive pressure to drive gas flow in a lung-on-a-chip to simulate the respiratory process. The structure of pressure supply devices is also diverse and is known to those skilled in the art. Pressure supply devices may include structures such as gas cylinders, connecting pipes, proportional valves, pressure gauges, and air pumps, and are not limited in this application.

[0067] It should be noted that the gas guiding assembly 140 has a first gas port 140a and a second gas port 140b. It should also be noted that the gas guiding assembly 140 has multiple first gas ports 140a and multiple second gas ports 140b. Some of the first gas ports 140a of the gas guiding assembly 140 are connected to the negative pressure output port of the pressure supply device, and the second gas ports 140b connected to these first gas ports 140a output negative pressure; other first gas ports 140a are connected to the positive pressure output port of the pressure supply device, and the second gas ports 140b connected to these first gas ports 140a output positive pressure. The multiple second gas ports 140b on the gas guiding assembly 140 are correspondingly arranged one-to-one with the multiple gas path interfaces 2000a on the culture box 2000. It should be noted that, along a third direction, the second gas ports 140b of the gas guiding assembly 140 are opposite to the gas path interfaces 2000a of the culture box 2000.

[0068] The first driving mechanism 150 drives the air guiding component 140 to move upward or downward in the third direction. When the air guiding component 140 moves downward in the third direction under the drive of the first driving mechanism 150, the second air port 140b of the air guiding component 140 can be communicated with the air path interface 2000a of the culture box 2000, so that the air path interface 2000a of the culture box 2000 and the air port of the pressure supply device are indirectly communicated. When the air guiding component 140 moves upward in the third direction under the action of the first driving mechanism 150, the second air port 140b of the air guiding component 140 is disconnected from the air path interface 2000a on the culture box 2000, so that the air path interface 2000a of the culture box 2000 and the air port of the pressure supply device are disconnected.

[0069] The culture device 100 drives the air guiding component 140 to move through the first driving mechanism 150, so that the air path interface 2000a of the culture box 2000 is communicated with or disconnected from the second air port 140b of the air guiding component 140. After such a design, there is no need to manually dock or disconnect the culture box 2000 and the pressure supply device, and the connection or disconnection of the culture box 2000 and the pressure supply device is more convenient, and the risk of contamination of the organ chip in the culture box 2000 is also reduced.

[0070] The air guiding component 140 is arranged between the first upper bearing platform 1211 and the bracket 122, and the culture box 2000 is arranged between the bracket 122 and the air guiding component 140. Both the air guiding component 140 and the culture box 2000 are in the lower area of the first upper bearing platform 1211. After such a design, when dust and other particulate matters in the air move downward, the first upper bearing platform 1211 will block them, so that most of the dust and other particulate matters cannot cross the first upper bearing platform 1211 and fall on the air guiding component 140 or the culture box 2000. Therefore, the first upper bearing platform 1211 can play a role in blocking dust, reducing the possibility of dust adhering to the surface of the culture box 2000 or the air guiding component 140, ensuring the cleanliness of the air guiding component 140 and the culture box 2000, and helping to maintain suitable culture conditions in the culture box 2000.

[0071] The structures of the first support frame 1212, the first upper bearing platform 1211 and the first upper bearing platform 1211 are diverse and are not limited in this application.

[0072] In some embodiments, both the first upper bearing platform 1211 and the first upper bearing platform 1211 are in a rectangular plate-like structure.

[0073] In some embodiments, the first support frame 1212 is in a "field" shape.

[0074] In some embodiments, the first base 110 further includes a guide rod 130, which is disposed along a third direction and connected to the first support frame 1212. The air guide assembly 140 is slidably connected to the guide rod 130 along a third direction.

[0075] The guide rod 130 is fixedly connected to the first support frame 1212. The fixed connection can be achieved in various ways, such as welding, snap-fitting, or bolting. After the guide rod 130 is installed, the gas guiding assembly 140 is slidably connected to the guide rod 130 along the third direction. This helps improve the stability of the gas guiding assembly 140's movement along the third direction, ensuring that when the gas guiding assembly 140 moves downwards along the third direction, its second air port 140b can be accurately aligned with the air passage interface 2000a of the culture box 2000.

[0076] In some embodiments, the first drive mechanism 150 includes a reset member 160 and a drive assembly 170. The reset member 160 acts on the air guide assembly 140 and the first support frame 1212, applying a force to the air guide assembly 140 to move it away from the bracket 122; the drive assembly 170 is mounted on the first upper support platform 1211, and the output end of the drive assembly 170 acts on the air guide assembly 140 to drive the air guide assembly 140 to move toward the bracket 122, so that the second air port 140b communicates with the air passage interface 2000a.

[0077] The first upper support platform 1211 supports the drive assembly 170. Under the coordinated action of the reset member 160 and the drive assembly 170, the gas guide assembly 140 can move downwards along a third direction, connecting its second air port 140b with the air passage interface 2000a on the culture box 2000, thereby indirectly connecting the air passage interface 2000a of the culture box 2000 with the air port of the pressure supply device. Under the coordinated action of the reset member 160 and the drive assembly 170, the gas guide assembly 140 can also move upwards along a third direction, disconnecting its second air port 140b from the air passage interface 2000a on the culture box 2000, thereby disconnecting the air passage interface 2000a of the culture box 2000 from the air port of the pressure supply device.

[0078] Specifically, one side of the reset member 160 acts on the gas guiding assembly 140, and the other side acts on the first support frame 1212. The reset member 160 applies a force away from the support frame 122 to the gas guiding assembly 140, causing the gas guiding assembly 140 to move upward in a third direction, moving it away from the culture box 2000, thereby disconnecting the second air port 140b of the gas guiding assembly 140 from the air passage interface 2000a on the culture box 2000. The reset member 160 can be a spring, rubber band, rubber block, etc., and is not limited in this application.

[0079] Specifically, the drive assembly 170 drives the air guide assembly 140 to move towards the support 122, that is, drives the air guide assembly 140 to move downward in a third direction, so that the air guide assembly 140 approaches the culture box 2000, so that the second air port 140b of the air guide assembly 140 and the air passage interface 2000a of the culture box 2000 are connected. The drive assembly 170 can be a cylinder, a linear motor, a hydraulic cylinder, etc., and is not limited in this application. If the drive assembly 170 is a cylinder, the output end is the piston rod of the cylinder; similarly, if the drive assembly 170 is a hydraulic cylinder, the output end is the piston rod of the hydraulic cylinder. If the drive assembly 170 is a linear motor, the output end is the slider of the linear motor.

[0080] When the gas interface 2000a of the culture box 2000 needs to connect with the second gas port 140b of the gas guiding component 140, the drive component 170 applies force to the gas guiding component 140, causing the gas guiding component 140 to move downward along a third direction until the second gas port 140b of the gas guiding component 140 and the gas interface 2000a of the culture box 2000 are connected. During the downward movement of the gas guiding component 140 along the third direction, the reset member 160 deforms and stores force, and the gas guiding component 140 needs to overcome the force of the reset member 160. When the second gas port 140b of the gas guiding component 140 and the gas interface 2000a of the culture box 2000 are connected, the drive component 170 still needs to apply a certain amount of force to the gas guiding component 140. This force is greater than or equal to the force applied by the reset member 160 to the gas guiding component 140, so that the gas guiding component 140 can remain in the position where the second gas port 140b and the gas interface 2000a are connected.

[0081] When the gas interface 2000a of the culture box 2000 needs to be disconnected from the second gas port 140b of the gas guide assembly 140, the drive assembly 170 stops applying force to the gas guide assembly 140, or the force applied by the drive assembly 170 to the gas guide assembly 140 is less than the force applied by the reset member 160 to the gas guide assembly 140, the reset member 160 will push the gas guide assembly 140 to move upward in a third direction, so that the second gas port 140b of the gas guide assembly 140 and the gas interface 2000a of the culture box 2000 are disconnected.

[0082] The culture device 100 drives the gas guiding assembly 140 towards the culture box 2000 via the drive assembly 170, thereby connecting the gas interface 2000a of the culture box 2000 with the second gas port 140b of the gas guiding assembly 140, and indirectly connecting the gas interface 2000a of the culture box 2000 with the gas port of the pressure supply device. When the gas interface 2000a of the culture box 2000 is not connected to the gas port of the pressure supply device, the reset member 160 pushes the gas guiding assembly 140 away from the culture box 2000, thereby disconnecting the second gas port 140b of the gas guiding assembly 140 from the gas interface 2000a of the culture box 2000, and thus disconnecting the gas interface 2000a of the culture box 2000 from the gas port of the pressure supply device.

[0083] In some embodiments, the reset member 160 is sleeved outside the guide rod 130.

[0084] With this design, the guide rod 130 can provide limit and guide for the reset member 160, preventing the reset member 160 from shifting laterally or shaking when subjected to force, and ensuring that the air guide assembly 140 can move upward in a third direction.

[0085] like Figure 7 , Figure 8 and Figure 9 As shown, in some embodiments, the drive assembly 170 includes: a support 174, a drive member 171, a swing assembly 172, and an output member 173. The support 174 is mounted on a first upper support platform 1211; the drive member 171 is mounted on the support 174; the swing assembly 172 has a first end and a second end, the first end being connected to the output shaft 1711 of the drive member 171; the output member 173 is slidably connected to the support 174 along a third direction, and the output member 173 is located between the swing assembly 172 and the air guide assembly 140, the second end of the swing assembly 172 acts on the output member 173, and the output member 173 acts on the air guide assembly 140.

[0086] The support 174 serves as the mounting base for the drive component 171 and the output component 173. The support 174 supports the drive component 171 and provides guidance for the output component 173. The structure of the support 174 is varied and is not limited in this application. The first end of the swing assembly 172 is fixedly connected to the output shaft 1711 of the drive component 171. The output shaft 1711 of the drive component 171 drives the swing assembly 172 to swing towards or away from the output component 173.

[0087] When the swing assembly 172 swings toward the output component 173, the second end of the swing assembly 172 abuts against the output component 173 and drives the output component 173 to move downward along a third direction. The output component 173 drives the force-receiving component 143 to move downward along a third direction, so that the entire gas guide assembly 140 moves downward along a third direction, thereby connecting the second air port 140b of the gas guide assembly 140 with the gas passage interface 2000a of the culture box 2000. When the second air port 140b of the gas guide assembly 140 is connected to the gas passage interface 2000a of the culture box 2000, the drive component 171 stops rotating, and the output shaft 1711 of the drive component 171 remains in the stopped position, so that the swing assembly 172 remains in the stopped position of the drive component 171, thereby enabling the gas guide assembly 140 to remain in the position where the second air port 140b and the gas passage interface 2000a are connected.

[0088] When the swing assembly 172 swings away from the output component 173, the gas guide assembly 140 moves upward along a third direction under the force of the reset member 160, thereby disconnecting the second air port 140b of the gas guide assembly 140 from the air passage interface 2000a of the culture box 2000. During the upward movement of the gas guide assembly 140 along the third direction, the gas guide assembly 140 pushes the output component 173 to move upward along the third direction, thereby resetting the output component 173.

[0089] The drive component 171 can be a stepper motor with a brake. When the power is off, the output shaft 1711 is locked by the brake device and will not rotate due to external force.

[0090] In some embodiments, the output shaft 1711 of the drive member 171 is perpendicular to a third direction.

[0091] In some embodiments, the output shaft 1711 of the drive member 171 is arranged along a first direction.

[0092] In some embodiments, the oscillating assembly 172 includes a swing arm 1721 and a rotating wheel 1722. One end of the swing arm 1721 is connected to the output shaft 1711 of the drive member 171; the rotating wheel 1722 is rotatably connected to the other end of the swing arm 1721, and the rotating wheel 1722 acts on the output member 173.

[0093] In these embodiments, the end of the swing arm 1721 connected to the output shaft 1711 is the first end of the swing assembly 172, and the rotating wheel 1722 is the second end of the swing assembly 172. It should be noted that the swing arm 1721 and the output shaft 1711 are set at an angle, such as an obtuse angle, an acute angle, or a right angle. The swing arm 1721 and the output shaft 1711 are fixedly connected. The output shaft 1711 drives the swing arm 1721 to rotate, and the swing arm 1721 drives the rotating wheel 1722 to rotate around the output shaft 1711. The rotating wheel 1722 pushes the output component 173 to move downwards in a third direction. During the process of the rotating wheel 1722 pushing the output component 173, the rotating wheel 1722 rotates on its own axis, and the circumferential surface of the rotating wheel 1722 makes rolling contact with the output component 173, resulting in less friction and improving the stability of the output component 173.

[0094] In some embodiments, the swing arm 1721 is perpendicular to the output shaft 1711, and the axis of rotation of the wheel 1722 is parallel to the output shaft 1711.

[0095] like Figure 3 As shown, in some embodiments, the support 174 is disposed above the first upper support platform 1211, the first upper support platform 1211 is provided with a clearance through hole 1211a, and the output component 173 is located in the clearance through hole 1211a.

[0096] The clearance through hole 1211a is used to avoid the output component 173. With this design, the output component 173 passes through the first upper support platform 1211 through the clearance through hole 1211a to contact the air guide assembly 140.

[0097] like Figure 6 As shown, in some embodiments, the air guiding assembly 140 includes an air guiding member 141 and a connecting member 142. The air guiding member 141 has a first air port 140a and a second air port 140b, and the air guiding member 141 and the bracket 122 are disposed opposite to each other along a third direction; the connecting member 142 is connected to the air guiding member 141, and the connecting member 142 has a sliding hole 142a disposed along a third direction, and the connecting member 142 is slidably sleeved on the outside of the guide rod 130 through the sliding hole 142a.

[0098] The connector 142 and the air guide 141 are fixedly connected. The shape and size of the cross-section of the sliding hole 142a must be adapted to the shape and size of the cross-section of the guide rod 130, so that the connector 142 can be sleeved on the outside of the guide rod 130 through the sliding hole 142a, and the connector 142 can slide up and down along the guide rod 130 in a third direction. The output end of the reset member 160 can act on the air guide 141 or the connector 142, which is not limited in this application. The air guide assembly 140 includes two parts: the air guide 141 and the connector 142, so that the air guide 141 and the connector 142 can be processed separately and then assembled to form the air guide assembly 140, which facilitates the processing of the air guide assembly 140.

[0099] In some embodiments, the cross-sections of the sliding hole 142a and the guide rod 130 are both circular. The inner diameter of the sliding hole 142a is smaller than the outer diameter of the guide rod 130. The inner wall of the sliding hole 142a and the outer wall of the guide rod 130 are clearance-fitted to ensure that the guide rod 130 can be located inside the sliding hole 142a, and the connector 142 can slide up and down along the guide rod 130 in a third direction.

[0100] In some embodiments, the first base 110 includes a plurality of guide rods 130 spaced apart, and the air guiding assembly 140 includes a plurality of connectors 142, the number of which is the same as the number of guide rods 130, and the connectors 142 are configured in a one-to-one correspondence with the guide rods 130. Each connector 142 slides on its corresponding guide rod 130. This design helps to reduce the swaying or offset of the air guiding assembly 140 during sliding, and improves the stability of the air guiding assembly 140 during sliding.

[0101] In some embodiments, the air guide 141 is cuboid in shape, with its long side parallel to the first direction and its height parallel to the third direction. A second air port 140b is disposed on the end face of the air guide 141 facing the bracket 122. First air ports 140a are disposed on two opposite end faces along the first direction.

[0102] In some embodiments, along a third direction, both the guide rod 130 and the connector 142 are located below the first upper support platform 1211.

[0103] With this design, when dust and other particles in the air move downwards, the first upper support platform 1211 will block them, preventing most of the dust and other particles from crossing the first upper support platform 1211 and falling between the guide rod 130 and the sliding hole 142a. This helps to improve the smoothness of the air guiding assembly 140 sliding along the guide rod 130.

[0104] In some embodiments, the air guiding assembly 140 is provided with two connectors 142, which are respectively disposed on both sides of the air guiding assembly 141 along the first direction and located on the side of the air guiding assembly 141 closer to the first upper support platform 1211. With this design, both sides of the air guiding assembly 140 along the first direction are slidably connected to the guide rod 130, which helps to improve the stability of the air guiding assembly 140 moving along the third direction.

[0105] In some embodiments, the air guiding assembly 140 further includes a force-receiving member 143. The force-receiving member 143 is connected to the air guiding member 141 and is disposed on the side of the air guiding member 141 near the first upper support platform 1211 along a third direction, and is located between two connecting members 142. The output member 173 acts on the air guiding assembly 140 and acts on the force-receiving member 143.

[0106] The force-bearing component 143 is fixedly connected to the air guide component 141. The output component 173 of the drive assembly 170 acts on the force-bearing component 143, pushing the force-bearing component 143 to move downward in a third direction. The force-bearing component 143 drives the air guide component 141 to move downward in a third direction. The output end of the drive assembly 170 acts on the force-bearing component 143, avoiding direct contact with the air guide component 141, which helps to prevent the drive assembly 170 from damaging the air guide component 141. The force-bearing component 143 is disposed between the two connecting components 142, utilizing the area between the two connecting components 142, which helps to reduce the overall volume of the air guide assembly 140.

[0107] The force-bearing component 143 can be provided in one or more ways, and no limitation is made in this application. In some embodiments, a force-bearing component 143 is provided, and the force-bearing component 143 is located in the middle of the air guide component 141 along the first direction.

[0108] In some embodiments, the force-receiving member 143 includes a force-receiving part 1431 and a force-transmitting part 1432. The force-transmitting part 1432 is disposed along a third direction. Along the third direction, the lower end of the force-transmitting part 1432 is connected to the air guide member 141, and the upper end is connected to the force-receiving part 1431. The force-transmitting part 1432 is in the shape of a round rod, and the force-receiving part 1431 is in the shape of a disc. The output end of the drive assembly 170 acts on the force-receiving part 1431.

[0109] In some embodiments, the first base 110 includes two brackets 122 spaced apart along a second direction, and the culture device 100 includes two gas guiding components 140 spaced apart along a second direction, with the gas guiding components 140 and the brackets 122 arranged in a one-to-one correspondence.

[0110] With this design, culture boxes 2000 can be placed on both brackets 122, allowing the culture device 100 to culture as many organ-on-a-chip as possible at once, thus improving culture efficiency. In these embodiments, two first drive mechanisms 150 can be provided, with each of the two first drive mechanisms 150 corresponding to one of the two gas guiding components 140, so as to achieve independent control of the two gas guiding components 140.

[0111] like Figure 3 As shown, in some embodiments, the bracket 122 has a plurality of support portions 110a spaced apart along a first direction, and the first lower support platform 1213 has a first slide groove 121a arranged along the first direction, and the bracket 122 is slidably disposed in the first slide groove 121a along the first direction.

[0112] With this design, the bracket 122 can support multiple culture boxes 2000 simultaneously. The first sliding groove 121a on the frame 121 provides sliding space for the bracket 122 and serves as a guide. In actual operation, multiple culture boxes 2000 are simply placed on the bracket 122 in sequence, and then the bracket 122 is pushed into the first sliding groove 121a along the first direction to complete the installation of multiple culture boxes 2000. When it is necessary to remove the culture boxes 2000, simply pull the bracket 122 out of the first sliding groove 121a to easily remove multiple culture boxes 2000, which facilitates the installation and removal of multiple culture boxes 2000 and improves the convenience of operation.

[0113] In some embodiments, the bracket 122 is provided with a handle 1221, which is located outside the first slide groove 121a. An operator can grasp the handle 1221 and apply force to the bracket 122 to move the bracket 122.

[0114] In some embodiments, the bottom wall of the first chute 121a has a third observation port 121b, and the bracket 122 has a fourth observation port 122a. Along the third direction, the third observation port 121b and the fourth observation port 122a are opposite to each other; the observation window 2000b of the culture box 2000 is exposed to the outside through the third observation port 121b and the fourth observation port 122a.

[0115] like Figure 5 As shown, the culture box 2000 has an observation window 2000b. Organ-on-a-chip (SoC) is placed in the culture box 2000 for culture, and the SoC can be seen through the observation window 2000b. The structure of the culture box 2000 with the observation window 2000b is known to those skilled in the art and is commercially available; therefore, it is not limited in this application. The observation window 2000b is exposed through a third observation port 121b and a fourth observation port 122a, facilitating observation of the SoC by operators using microscopes or other equipment.

[0116] like Figure 1 and Figure 6 As shown, in some embodiments, the first air port 140a and the second air port 140b are connected through a flow channel formed in the air guiding assembly 140. The air guiding assembly 140 also has an installation port 140c connected to the flow channel, and the installation port 140c is located between the first air port 140a and the second air port 140b. The culture device 100 also includes a solenoid valve 180, which is installed in the installation port 140c and is used to control the opening and closing of the flow channel.

[0117] With this configuration, when a certain second air port 140b does not need to discharge air, controlling the corresponding solenoid valve 180 will interrupt the corresponding flow path. When a certain second air port 140b needs to discharge air, controlling the corresponding solenoid valve 180 will open the corresponding flow path, facilitating the operation of the culture device 100. Furthermore, when the flow path is open, the flow rate of the second air port 140b can be adjusted by controlling the opening of the solenoid valve 180.

[0118] like Figure 10 As shown, based on the same inventive concept, this application embodiment also provides a culture system 1000, including the culture device 100 described above. Since the culture system 1000 includes the culture device 100 described above, it naturally has all the beneficial effects of the culture device 100, which will not be elaborated here.

[0119] like Figure 10 , Figure 11 and Figure 12 As shown, in some embodiments, the culture system 1000 further includes an imaging device 200, which includes a second base 210, an imaging module 220, and a second drive mechanism 230. The second drive mechanism 230 is mounted on the second base 210. A first base 110 is disposed on the second base 210. The bottom wall of the first slide 121a has a third observation port 121b, and the bracket 122 has a fourth observation port 122a. Along a third direction, the third observation port 121b and the fourth observation port 122a are opposite each other. The second base 210 has a second observation port 210a. Along a third direction, the second observation port 210a and the fourth observation port 122a are opposite each other. The second drive mechanism 230 drives the imaging module 220 to move so that the imaging module 220 is opposite to the observation window 2000b of the culture box 2000 in a third direction.

[0120] With this design, the observation window 2000b of the culture box 2000 is exposed to the outside through the second observation port 210a, the third observation port 121b and the fourth observation port 122a.

[0121] The imaging module 220 can be a microscope, a digital camera, a microscopic imaging system, etc., and is not limited thereto in this application. The structures of microscopes and digital cameras are known to those skilled in the art and will not be described in detail here. The microscopic imaging system combines microscopy and digital imaging technology, converting the images observed under the microscope into digital signals through an image sensor, and then displaying and storing them in real time. Its structure is also known to those skilled in the art.

[0122] The second drive mechanism 230 can be driven by various methods, such as motor drive or pneumatic drive. Driven by the second drive mechanism 230, the imaging module 220 is movable. This design addresses two main issues: First, it can accommodate placement deviations in the culture box 2000. In actual operation, the culture box 2000 may have positional deviations, causing the observation window 2000b to misalign with the imaging module 220. The second drive mechanism 230 can drive the imaging module 220 to make fine adjustments, aligning it with the observation window 2000b and ensuring accurate and stable imaging. Second, it can meet the needs of multi-region observation. The culture box 2000 often contains multiple areas requiring observation. For example, in organ-on-a-chip culture, different types of cells or tissues at different growth stages may be cultured in different locations. The second drive mechanism 230 drives the imaging module 220 to move sequentially to each observation area, aligning it with the corresponding observation window 2000b, achieving sequential imaging and acquiring images of cell morphology and growth status in each area, providing comprehensive data for scientific research.

[0123] like Figure 12 As shown, in some embodiments, the second driving mechanism 230 includes a first driving component 240, a second driving component 250, and a third driving component 260. An imaging module 220 is mounted on the output end of the first driving component 240, and the first driving component 240 drives the imaging module 220 to move along a third direction. The first driving component 240 is mounted on the output end of the second driving component 250, and the second driving component 250 drives the first driving component 240 to move along a second direction. The third driving component 260 is mounted on the second base 210, and the second driving component 250 is mounted on the output end of the third driving component 260, and the third driving component 260 drives the second driving component 250 to move along a first direction.

[0124] The first driving component 240 is mounted at the output end of the second driving component 250, and the second driving component 250 drives the first driving component 240 to move in the second direction, thus enabling the imaging module 220 on the first driving component 240 to move in the second direction. Similarly, the second driving component 250 is mounted at the output end of the third driving component 260. When the third driving component 260 drives the second driving component 250 to move in the first direction, it drives the first driving component 240 and the imaging module 220 on the first driving component 240 to move in the first direction. The first driving component 240 can then drive the imaging module 220 to move in the third direction.

[0125] With this design, controlling the first drive component 240 adjusts the position of the imaging module 220 in a third direction; controlling the second drive component 250 adjusts the position of the imaging module 220 in a second direction; and controlling the third drive component 260 controls the position of the imaging module 220 in the first direction. This design allows the imaging module 220 to move in only one direction or simultaneously in multiple directions, resulting in higher position adjustment efficiency. It also shortens the alignment time between the imaging module 220 and the observation window 2000b of the culture chamber 2000, thus improving the observation efficiency of organ-on-a-chip.

[0126] In some embodiments, the culture apparatus 100 can support multiple culture boxes 2000, which are spaced apart along a first direction and also spaced apart along a second direction. In these embodiments, the imaging module 220 can be positioned sequentially opposite the observation window 2000b of each culture box 2000 by controlling the operation of the second driving component 250 and the third driving component 260, so as to observe the organ-on-a-chip in each culture box 2000. The spacing between the imaging module 220 and the organ-on-a-chip in the culture box 2000 can be adjusted by controlling the operation of the first driving component 240, so that the organ-on-a-chip can be clearly observed.

[0127] like Figure 13 As shown, in some embodiments, the first driving assembly 240 includes a first support platform 241 and a first driving member 242. The first support platform 241 is mounted on the output end of the second driving assembly 250. The first driving member 242 is mounted on the first support platform 241, and the imaging module 220 is mounted on the output end of the first driving member 242.

[0128] A first support platform 241 supports a first driving component 242. The first driving component 242 drives the imaging module 220 to move along a third direction. The first support platform 241 is fixedly connected to the output end of the second driving assembly 250. The first driving component 242 can be a cylinder, a hydraulic cylinder, a linear motor, etc. If the first driving component 242 is a cylinder, its output end is the piston rod of the cylinder; similarly, if the first driving component 242 is a hydraulic cylinder, its output end is the piston rod of the hydraulic cylinder. If the first driving component 242 is a linear motor, its output end is the slider of the linear motor.

[0129] In some embodiments, the second drive assembly 250 includes a second support platform 251 and a second drive member 252. The second support platform 251 is mounted on the output end of the third drive assembly 260; the second drive member 252 is mounted on the second support platform 251, and the first drive assembly 240 is mounted on the output end of the second drive member 252.

[0130] The second support platform 251 supports the second driving member 252 thereon. The second driving member 252 drives the first driving assembly 240 to move along the second direction. The second support platform 251 is fixedly connected to the output end of the third driving assembly 260. The second driving member 252 can be a cylinder, a hydraulic cylinder, a linear motor, etc., and is not limited in this application. If the second driving member 252 is a cylinder, the output end of the second driving member 252 is the piston rod of the cylinder; similarly, if the second driving member 252 is a hydraulic cylinder, the output end of the second driving member 252 is the piston rod of the hydraulic cylinder. If the second driving member 252 is a linear motor, the output end of the second driving member 252 is the slider of the linear motor.

[0131] In an embodiment where the first drive assembly 240 includes a first support platform 241 and a first drive member 242, the first support platform 241 is fixedly connected to the output end of the second drive member 252.

[0132] In some embodiments, the third drive assembly 260 includes a third drive member 261 and a sliding seat 262. The third drive member 261 is mounted on the second base 210, and one side of the second support platform 251 is connected to the output end of the third drive member 261; the sliding seat 262 is mounted on the second base 210, and the other side of the second support platform 251 is slidably connected to the sliding seat 262 along a first direction.

[0133] Both the sliding seat 262 and the third driving member 261 are fixedly connected to the second base 210, and the second support platform 251 is fixedly connected to the output end of the third driving member 261. The third driving member 261 drives the second support platform 251 to move along a first direction. The sliding seat 262 provides guidance for the second support platform 251, enabling the second support platform 251 to slide accurately along a second direction, thereby improving the stability of the second support platform 251 moving along the second direction. The third driving member 261 can be a cylinder, a hydraulic cylinder, a linear motor, etc., and is not limited in this application. If the third driving member 261 is a cylinder, the output end of the third driving member 261 is the piston rod of the cylinder; similarly, if the third driving member 261 is a hydraulic cylinder, the output end of the third driving member 261 is the piston rod of the hydraulic cylinder. If the third driving member 261 is a linear motor, the output end of the third driving member 261 is the slider of the linear motor.

[0134] like Figure 13 As shown, in some embodiments, the second base 210 includes a second lower support platform 211, a second upper support platform 212, and a support column 213. A second drive mechanism 230 is mounted on the second lower support platform 211; along a third direction, the second lower support platform 211 and the second upper support platform 212 are opposite each other, and a first base 110 is disposed on the second upper support platform 212; the support column 213 is disposed between the second lower support platform 211 and the second upper support platform 212, connecting the second lower support platform 211 and the second upper support platform 212.

[0135] The second lower support platform 211 supports the second drive mechanism 230, and the support column 213 supports the second upper support platform 212. One or more support columns 213 can be provided, and no limitation is made here.

[0136] In some embodiments, both the sliding seat 262 and the third drive member 261 are fixedly connected to the second lower support platform 211.

[0137] like Figure 12 As shown, in some embodiments, the second base 210 has a second slide groove 210b arranged along a first direction, the second observation port 210a is disposed on the bottom wall of the second slide groove 210b, and the first base 110 is slidably disposed in the second slide groove 210b along the first direction.

[0138] The second slide 210b provides guidance and limitation for the first base 110. When installing the first base 110, the first base 110 is aligned with the opening of the second slide 210b. After alignment, a force is applied to the first base 110, causing it to slide along the second slide 210b to the top of the first base 110, thus completing the installation. When the first base 110 needs to be removed, a force is applied to it, causing it to slide along the second slide 210b to the outside of the first base 110, thus completing the removal. With this design, the gas guiding device 100 can be detached from the imaging device 200, allowing the imaging device 200 to accommodate gas guiding devices 100 suitable for different culture boxes, improving the flexibility and versatility of the culture system 1000 and meeting diverse research needs.

[0139] The following describes the working principle of the culture system 1000:

[0140] When organ-on-a-chip culture is required, the handle 1221 is used to pull the tray 122 out of the first groove 121a, and the culture boxes 2000 containing the organ-on-a-chip are placed sequentially in the corresponding positions on the tray 122. Next, the handle 1221 is held and force is applied to the tray 122, causing it to be pushed into the first groove 121a along a third direction, so that the culture boxes 2000 are positioned below the gas delivery assembly 140, and the third observation port 121b and the fourth observation port 122a are aligned along a second direction. Then, the first air port 140a of the gas delivery assembly 140 is indirectly connected to the air port of the pressure supply device via a pipe or similar means. Next, the drive component 171 is controlled to rotate, and the output shaft 1711 of the drive component 171 drives the swing assembly 172 toward the output component 17. The oscillating component 172 drives the output component 173 to move downward along the third direction, and the output component 173 drives the force-receiving component 143 to move downward along the third direction, so that the entire gas guiding component 140 moves downward along the third direction until the second air port 140b of the gas guiding component 140 and the gas passage interface 2000a of the culture box 2000 are connected; when the second air port 140b of the gas guiding component 140 and the gas passage interface 2000a of the culture box 2000 are connected, the driving component 171 stops rotating, and the output shaft 1711 of the driving component 171 remains in the position when it stops, so that the oscillating component 172 remains in the position when the driving component 171 stops, thereby keeping the gas guiding component 140 in the position where the second air port 140b and the gas passage interface 2000a are connected.

[0141] If it is necessary to observe the organ-on-a-chip during the culture process, the second driving component 250 and the third driving component 260 are controlled to move so that the imaging module 220 and the organ-on-a-chip in the corresponding culture box 2000 are relative to each other in a third direction; then, the first driving component 240 is controlled to move to adjust the distance between the imaging module 220 and the organ-on-a-chip, and finally the organ-on-a-chip is observed through the imaging module 220.

[0142] When organ-on-a-chip culture is complete, and it is necessary to disconnect the second air port 140b of the gas delivery assembly 140 and the air path interface 2000a of the culture box 2000, the drive component 171 is controlled to rotate in the opposite direction, and the swing component 172 will swing away from the output component 173. Under the action of the reset component 160, the gas delivery assembly 140 moves upward along the third direction, and the force-bearing component 143 pushes the output component 173 to move upward along the third direction to reset. Finally, the grip handle 1221 pulls the bracket 122 out from the first slide groove 121a and removes the multiple culture boxes 2000 on the bracket 122.

[0143] When the air guide device 100 needs to be replaced, force is applied to the air guide device 100 to pull it out of the second slide groove 210b, and then the replaced air guide device 100 is placed in the second slide groove 210b.

[0144] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application, and are not intended to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this application.

Claims

1. A culture device, characterized in that, include: The first base (110) includes a bracket (122), a first upper support platform (1211), a first support frame (1212), and a first lower support platform (1213). The first upper support platform (1211) and the first lower support platform (1213) are arranged opposite to each other along a third direction. The first support frame (1212) is connected to the first upper support platform (1211) and the first lower support platform (1213). The bracket (122) is disposed on the first lower support platform (1213) and has a support portion (110a) for supporting the culture box (2000). An air guide assembly (140) has a communicating first air port (140a) and a second air port (140b) and is located between the first upper support platform (1211) and the bracket (122), with the air guide assembly (140) and the bracket (122) facing each other along the third direction; A first drive mechanism (150) is installed on the first upper support platform (1211) to drive the gas guide assembly (140) to move along the third direction, so that the second air port (140b) of the gas guide assembly (140) is connected or disconnected from the air passage interface (2000a) of the culture box (2000).

2. The culture apparatus according to claim 1, characterized in that, The first base (110) also includes: A guide rod (130) is provided along the third direction and connected to the first support frame (1212). The air guiding assembly (140) is slidably connected to the guide rod (130) along the third direction.

3. The culture apparatus according to claim 2, characterized in that, The first drive mechanism (150) includes: The reset component (160) is sleeved outside the guide rod (130) and acts on the air guide assembly (140) and the first support frame (1212), applying a force to the air guide assembly (140) to make it move away from the bracket (122); A drive assembly (170) is installed on the first upper support platform (1211). The output end of the drive assembly (170) acts on the air guide assembly (140) to drive the air guide assembly (140) to move toward the bracket (122) so that the second air port (140b) is connected to the air passage interface (2000a).

4. The culture apparatus according to claim 3, characterized in that, The drive component (170) includes: Support (174) is installed on the first upper bearing platform (1211); A drive component (171) is mounted on the support (174); The swing assembly (172) has a first end and a second end, the first end being connected to the output shaft (1711) of the drive member (171); The output component (173) is slidably connected to the support (174) along the third direction, and the output component (173) is located between the swing assembly (172) and the air guide assembly (140). The second end of the swing assembly (172) acts on the output component (173), and the output component (173) acts on the air guide assembly (140).

5. The culture apparatus according to claim 4, characterized in that, The support (174) is disposed above the first upper support platform (1211), and the first upper support platform (1211) is provided with a clearance through hole (1211a), and the output component (173) is located in the clearance through hole (1211a).

6. The culture apparatus according to claim 3, characterized in that, The air guiding assembly (140) includes: An air guide (141) has a first air port (140a) and a second air port (140b), and the air guide (141) and the bracket (122) are arranged opposite to each other along the third direction; A connector (142) is connected to the air guide (141). The connector (142) has a sliding hole (142a) arranged along the third direction. The connector (142) is slidably sleeved on the guide rod (130) through the sliding hole (142a).

7. The culture apparatus according to claim 6, characterized in that, Along the third direction, the guide rod (130) and the connector (142) are both located below the first upper support platform (1211).

8. The culture apparatus according to claim 6, characterized in that, The air guiding assembly (140) is provided with two connectors (142), which are respectively located on both sides of the air guiding component (141) along the first direction and on the side of the air guiding component (141) close to the first upper support platform (1211). The first direction is perpendicular to the third direction.

9. The culture apparatus according to claim 8, characterized in that, The air guiding assembly (140) also includes: The force-bearing component (143) is connected to the air guide component (141) along the third direction. The force-bearing component (143) is located on the side of the air guide component (141) close to the first upper support platform (1211) and between the two connecting components (142). The output end of the drive assembly (170) acts on the force-bearing component (143).

10. The culture apparatus according to any one of claims 1-9, characterized in that, The bracket (122) has a plurality of bearing portions (110a) spaced apart along a first direction, and the first lower bearing platform (1213) has a first slide groove (121a) arranged along the first direction. The bracket (122) is slidably disposed in the first slide groove (121a) along the first direction, and the first direction is perpendicular to the third direction.

11. The culture apparatus according to claim 10, characterized in that, The bottom wall of the first chute (121a) has a third observation port (121b), and the bracket (122) has a fourth observation port (122a). Along the third direction, the third observation port (121b) and the fourth observation port (122a) are opposite to each other. The observation window (2000b) of the culture box (2000) is exposed to the outside through the third observation port (121b) and the fourth observation port (122a).

12. The culture apparatus according to any one of claims 1-9, characterized in that, The first air port (140a) and the second air port (140b) are connected by a flow channel formed in the air guiding assembly (140). The air guiding assembly (140) also has an installation port (140c) connected to the flow channel, and the installation port (140c) is located between the first air port (140a) and the second air port (140b). The culture device also includes a solenoid valve (180), which is installed in the mounting port (140c) and is used to control the opening and closing of the flow channel.

13. The culture apparatus according to any one of claims 1-9, characterized in that, The first base (110) includes two brackets (122) spaced apart along a second direction, and the culture device (100) includes two gas guiding components (140) spaced apart along the second direction. The gas guiding components (140) and the brackets (122) are arranged in a one-to-one correspondence, and the second direction is perpendicular to the third direction.

14. A culture system, characterized in that, The culture apparatus (100) includes any one of claims 1-13.

Images