A cell and tissue transculture instrument

Abstract

This utility model relates to the field of cell culture technology and discloses a cell and tissue transduction culture instrument, including: a base plate, culture chambers, a drive assembly, and several transduction structures. The drive assembly and transduction structures are sequentially fixed to the upper end of the base plate. The transduction structures are used to drive the culture chambers to rotate. Each transduction structure includes a second mounting plate, a second bearing seat, a rotating shaft, a second fixed wheel, and a driven pulley. Four second bearing seats are fixed at intervals to the upper end of the second mounting plate. Each rotating shaft has its two ends mounted on a second bearing seat. Two second fixed wheels are fixed to their respective rotating shafts. The driven pulley is fixed to the outer side of the second fixed wheel. The culture chamber is located between the inner sides of two rotating shafts. The drive assembly includes a first mounting plate, a geared motor, a motor frame, a first bearing seat, a transmission shaft, a first fixed wheel, a drive pulley, and a transmission belt. This utility model offers high culture efficiency, flexible selection, and simple and convenient operation.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a cell and tissue transculture instrument. Background Technology

[0002] The formation, maintenance, and repair of biological tissues depend on interactions between cells and between cells and the extracellular matrix. Rotational cell culture effectively simulates the growth of human and animal tissue cells within their parent tissues, and is therefore widely used in medical and cell biology research. This includes generating highly realistic tissue models or long-term in vitro tissue culture. Its advantages include the ability to produce high-density, three-dimensional tissue cultures with differentiation capabilities; and the ability to promote membrane oxygenation, eliminate air bubbles, and create an optimal, mild culture environment.

[0003] Existing technologies also include some cell culture rotary culture devices, such as the Synthecon cell culture rotary culture device from the United States. This device uses a set of electric motors to rotate one culture chamber at a time, and the entire device can be placed in a CO2 incubator to maintain a stable environment during culture. However, it can only rotate one culture chamber of the same type at a time, resulting in low culture efficiency. Another example is the Chinese invention patent application CN202010366845.8, entitled "A Rotary Radial Spreading Axial Multi-Sheet Adherent Cell Culture Device." This cell culture device is used to maintain the flow of culture medium during culture, offering advantages such as strong mass transfer capacity, high uniformity of the growth environment, and good biomechanical environment simulation. However, it requires the use of a matching rotating device. Utility Model Content

[0004] In order to overcome the shortcomings of the existing technology, the purpose of this utility model is to provide a cell and tissue transfection culture instrument with high culture efficiency, flexible selection, and simple and convenient operation.

[0005] This utility model is achieved using the following technical solution: a cell tissue transfer culture instrument, comprising: a base plate, a culture chamber, a drive assembly, and several transfer structures. The drive assembly and the transfer structures are sequentially fixed to the upper end of the base plate. The transfer structures are used to drive the culture chamber to rotate. Each transfer structure includes a second mounting plate, a second bearing seat, a rotating shaft, a second fixed wheel, and a driven pulley. Four second bearing seats are fixed at intervals to the upper end of the second mounting plate. Both ends of each rotating shaft are respectively mounted on the second bearing seat. Two second fixed wheels are respectively fixed on the corresponding rotating shafts. The driven pulley is fixed to the outside of the second fixed wheel. The culture chamber is disposed between the inner sides of two rotating shafts.

[0006] Furthermore, the drive assembly includes a first mounting plate, a geared motor, a motor frame, a first bearing housing, a drive shaft, a first fixed pulley, a drive pulley, and a drive belt. The geared motor is fixed to one upper end of the first mounting plate via the motor frame, the first bearing housing is fixed to the other upper end of the first mounting plate, one end of the drive shaft is connected to the shaft of the geared motor, and the other end of the drive shaft is rotatably mounted on the first bearing housing. The first fixed pulley is fixed to the drive shaft, and the drive pulley is fixed to the outside of the first fixed pulley. The drive belt is sleeved on the driven pulley and the drive pulley.

[0007] Furthermore, a rotary joint is fixed to the outside of the shaft for manual rotation of the shaft.

[0008] Furthermore, the culture chamber includes a chamber body and chamber connecting plates symmetrically arranged at both ends of the chamber body. The front end of the rotating shaft is a hollow shaft with a groove that can accommodate the chamber connecting plates. A fastening knob is also installed on the outer side of the front end of the rotating shaft through a threaded engagement. The front threaded post of the fastening knob extends into the hollow shaft at the front end of the rotating shaft to fix the chamber connecting plates.

[0009] Furthermore, both the second mounting plate and the first mounting plate are provided with a plurality of mounting holes, and the second mounting plate and the first mounting plate are fixed to the base plate by screws passing through the mounting holes.

[0010] Furthermore, the aforementioned rotating pipe structure has 2 to 4 sets.

[0011] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0012] 1. The present invention relates to a cell and tissue transfection instrument, which drives a pulley to rotate via a reduction motor in the drive assembly and uses a transmission belt to drive up to 3 transfection structures to rotate at one time, thereby performing rotation culture in 3 culture chambers, resulting in higher culture efficiency and more flexible selection.

[0013] 2. The cell and tissue transfer culture instrument of this utility model can be manually rotated using a rotary joint so that the groove at the front end of the shaft faces upward or toward the operator, and then the chamber connecting plate is fixed by the threaded post at the front of the fastening knob. Its operation is simple and convenient. Attached Figure Description

[0014] Figure 1 This is a top view of the cell and tissue transculture apparatus of this utility model;

[0015] Figure 2 This is a schematic diagram showing the connection between the drive assembly and a set of rotating pipe structures in this utility model;

[0016] Figure 3 This is a schematic diagram of the rotating pipe structure of this utility model after removing the second mounting plate and the second bearing seat;

[0017] Figure 4 This is a schematic diagram of the connection structure between the culture chamber and the rotating shaft in this utility model.

[0018] In the diagram: Base plate-1; Culture chamber-3; Second mounting plate-21; Second bearing seat-22; Rotary joint-23; Rotating shaft-24; Second fixed wheel-25; Driven pulley-26; Transmission belt-27; Fastening knob-28; First mounting plate-30; Gear motor-31; Motor frame-32; First bearing seat-33; Transmission shaft-34; First fixed wheel-35; Drive pulley-36; Chamber body-41; Chamber connecting plate-42; Groove-241. Detailed Implementation

[0019] The following specific examples illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification. This utility model can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of this utility model. It should be noted that, unless otherwise specified, the following embodiments and features described therein can be combined with each other.

[0020] The purpose of this invention is to address the shortcomings of existing technologies by providing a cell and tissue transculture instrument.

[0021] Example 1

[0022] This embodiment provides a cell and tissue transfer culture instrument, referring to... Figures 1-3 As shown, the system includes: a base plate 1, a culture chamber 3, a drive assembly, and several rotating tube structures. The drive assembly and rotating tube structures are sequentially fixed to the upper end of the base plate 1. The rotating tube structures are used to drive the culture chamber 3 to rotate. There can be 2 to 4 sets of rotating tube structures, or more. In this embodiment, there are 3 rotating tube structures. The rotating tube structure includes a second mounting plate 21, a second bearing seat 22, a rotating shaft 24, a second fixed wheel 25, and a driven pulley 26. Four second bearing seats 22 are fixed at intervals to the upper end of the second mounting plate 21. Each rotating shaft 24 has its two ends mounted on the second bearing seat 22. Two second fixed wheels 25 are fixed to the corresponding rotating shaft 24. The driven pulley 26 is fixed to the outside of the second fixed wheel 25. The culture chamber 3 is located between the inner sides of two rotating shafts 24. A rotary joint 23 is fixed to the outside of the rotating shaft 24 for manual rotation of the rotating shaft 24.

[0023] Reference Figures 1-3As shown, the drive assembly includes a first mounting plate 30, a geared motor 31, a motor frame 32, a first bearing housing 33, a drive shaft 34, a first fixed pulley 35, a drive pulley 36, and a drive belt 27. The geared motor 31 is fixed to one end of the upper part of the first mounting plate 30 via the motor frame 32, and the first bearing housing 33 is fixed to the other end of the upper part of the first mounting plate 30. One end of the drive shaft 34 is connected to the shaft of the geared motor 31, and the other end of the drive shaft 34 is rotatably mounted on the first bearing housing 33. The first fixed pulley 35 is fixed on the drive shaft 34, and the drive pulley 36 is fixed to the outside of the first fixed pulley 35. The drive belt 27 is sleeved on the driven pulley 26 and the drive pulley 36. In this embodiment, the geared motor 31 uses a 12-24V DC geared motor from the prior art, with a minimum speed not exceeding 12 r / min and a maximum speed not less than 120 r / min. Both the second mounting plate 21 and the first mounting plate 30 are provided with several mounting holes. The second mounting plate 21 and the first mounting plate 30 are fixed to the base plate 1 by screws passing through the mounting holes, so that the drive assembly and the three rotating tube structures can be fixed independently, which facilitates the adjustment of the positional distance between the drive assembly and the three rotating tube structures, and facilitates installation and debugging.

[0024] Reference Figures 3-4 As shown, the culture chamber 3 includes a chamber body 41 and chamber connecting plates 42 symmetrically arranged at both ends of the chamber body 41. The front end of the rotating shaft 24 is a hollow shaft, and a groove 241 is provided on the front end of the rotating shaft 24, which can accommodate the chamber connecting plate 42. A fastening knob 28 is also installed on the outer side of the front end of the rotating shaft 24 through a threaded engagement. The front threaded post of the fastening knob 28 extends into the hollow shaft at the front end of the rotating shaft 24 to fix the chamber connecting plate 42, so that each culture chamber 3 is fixed between the corresponding two rotating shafts 24.

[0025] The present invention relates to a cell and tissue transfection culture instrument, which can be placed inside a carbon dioxide incubator in the prior art to maintain a relatively stable culture environment.

[0026] Before use, place the cells to be cultured inside the chamber body 41, and manually rotate the shaft 24 using the rotary joint 23 so that the groove 241 at the front end of the shaft 24 faces upward or toward the operator, facilitating the operation and fixing of the culture chamber 3. Specifically, insert the two chamber connecting plates 42 at both ends of the chamber body 41 into the groove 241, and then fix the chamber connecting plates 42 by the threaded post at the front of the fastening knob 28, so that the culture chamber 3 is fixed between the corresponding two rotating shafts 24.

[0027] During operation, the geared motor 31 in the drive assembly drives the three rotating tube structures to rotate via pulleys and transmission belts. It can drive three culture chambers 3 to rotate and culture at one time, resulting in higher culture efficiency. Of course, it can also drive one or two culture chambers to rotate and culture at one time, offering flexibility.

[0028] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A cell and tissue transculture instrument, characterized in that, include: The base plate (1), culture chamber (3), drive assembly, and several rotating tube structures are arranged in sequence. The drive assembly and rotating tube structures are fixed to the upper end of the base plate (1). The rotating tube structures are used to drive the culture chamber (3) to rotate. The rotating tube structures include a second mounting plate (21), a second bearing seat (22), a rotating shaft (24), a second fixed wheel (25), and a driven pulley (26). The four second bearing seats (22) are fixed at intervals to the upper end of the second mounting plate (21). The two ends of each rotating shaft (24) are respectively mounted on the second bearing seat (22). The two second fixed wheels (25) are respectively fixed on the corresponding rotating shaft (24). The driven pulley (26) is fixed to the outside of the second fixed wheel (25). The culture chamber (3) is arranged between the inner sides of the two rotating shafts (24).

2. The cell and tissue transculture apparatus according to claim 1, characterized in that: The drive assembly includes a first mounting plate (30), a geared motor (31), a motor frame (32), a first bearing seat (33), a drive shaft (34), a first fixed pulley (35), a drive pulley (36), and a drive belt (27). The geared motor (31) is fixed to one upper end of the first mounting plate (30) via the motor frame (32). The first bearing seat (33) is fixed to the other upper end of the first mounting plate (30). One end of the drive shaft (34) is connected to the shaft of the geared motor (31), and the other end of the drive shaft (34) is rotatably mounted on the first bearing seat (33). The first fixed pulley (35) is fixed on the drive shaft (34), and the drive pulley (36) is fixed to the outside of the first fixed pulley (35). The drive belt (27) is sleeved on the driven pulley (26) and the drive pulley (36).

3. The cell and tissue transculture apparatus according to claim 2, characterized in that: A rotary joint (23) is fixed on the outside of the shaft (24) for manually rotating the shaft (24).

4. The cell and tissue transculture apparatus according to claim 2 or 3, characterized in that: The culture chamber (3) includes a chamber body (41) and chamber connecting plates (42) symmetrically arranged at both ends of the chamber body (41). The front end of the rotating shaft (24) is a hollow shaft, and a groove (241) is provided on the front end of the rotating shaft (24). The groove (241) can accommodate the chamber connecting plate (42) to enter. A fastening knob (28) is also installed on the outer side of the front end of the rotating shaft (24) through a threaded fit. The front threaded post of the fastening knob (28) extends into the hollow shaft at the front end of the rotating shaft (24) to fix the chamber connecting plate (42).

5. The cell and tissue transculture apparatus according to claim 2, characterized in that: The second mounting plate (21) and the first mounting plate (30) are each provided with a number of mounting holes. The second mounting plate (21) and the first mounting plate (30) are fixed to the base plate (1) by screws passing through the mounting holes.

6. The cell and tissue transculture apparatus according to claim 2, characterized in that: The aforementioned rotating pipe structure has 2 to 4 sets.

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