A device for separating and purifying bioactive peptides

Abstract

This utility model discloses a bioactive peptide separation and purification device, belonging to the field of bioactive peptide separation and purification technology. Key technical features include a housing with a separation component inside. Both sides of the separation component are equipped with shaking components, each including a rubber connecting block. By using the separation component, the device performs graded filtration from coarse to fine through ultrafiltration, nanofiltration, and reverse osmosis membranes, avoiding efficiency degradation due to excessive load on a single membrane layer. Simultaneously, by precisely retaining substances of different molecular weights, it significantly improves the recovery rate and purity of bioactive peptides. Furthermore, when replacement is needed, the movable frame can be directly removed for individual cleaning or replacement, reducing maintenance costs and downtime. The shaking components generate high-frequency, small-amplitude vibrations in the separation component, preventing the accumulation of filter cake and avoiding membrane pore blockage, thereby maintaining stable filtration efficiency.

Description

Technical Field

[0001] This utility model relates to the field of bioactive peptide separation and purification technology, and in particular to a bioactive peptide separation and purification device. Background Technology

[0002] Bioactive peptides are a class of small molecule compounds with a variety of physiological functions and have broad application prospects in the fields of medicine, food, and cosmetics. In the industrial production of bioactive peptides, efficient separation and purification technology is a key link to ensure product quality and yield. At present, membrane separation technology is widely used in the separation and purification of bioactive peptides due to its advantages such as simple operation, high separation efficiency, and continuous production.

[0003] Existing multi-stage membrane separation devices for bioactive peptides have low extraction efficiency, serious resource waste, and cannot achieve full extraction, resulting in high investment costs.

[0004] The existing patent (publication number: CN213866030U) discloses a multi-stage membrane separation device for bioactive peptides. A stirring shaft is movably installed inside the third processing chamber, with multiple stirring rods fixed to the outside of the stirring shaft. A driven gear is fixed to the top of the stirring shaft. A rotating motor is located at the top of the third processing chamber, with a rotating shaft fixed to the right end of the motor and a driving gear fixed to the left end of the rotating shaft. The driving gear meshes with the driven gear, so that when the rotating motor is started, the driving gear drives the stirring shaft to rotate, thereby fully stirring the raw materials in the third processing chamber and improving the extraction rate.

[0005] To address the aforementioned issues, existing patents offer solutions. However, these multilayer membrane separation devices employ a fixed installation method, preventing independent disassembly and replacement of each membrane module. When a membrane module is damaged or severely contaminated, the entire membrane stack must be disassembled for processing, which is cumbersome and easily leads to the waste of other intact membrane modules. Furthermore, when processing raw materials with complex compositions, such as fermentation broth, a filter cake layer easily forms on the membrane surface, accompanied by concentration polarization, causing the membrane flux to drop rapidly. This necessitates frequent shutdowns for cleaning or replacement of membrane modules, significantly increasing maintenance costs and severely impacting production continuity.

[0006] To address this, a bioactive peptide separation and purification device is proposed. Utility Model Content

[0007] The purpose of this invention is to provide a bioactive peptide separation and purification device that solves the problems of existing separation devices that use a fixed installation method, making it impossible to independently disassemble and replace each membrane module. When a membrane layer is damaged or severely contaminated, the entire membrane stack needs to be disassembled for treatment, which is cumbersome and easily wastes other intact membrane modules. Furthermore, when processing raw materials with complex components such as fermentation broth, the membrane surface is prone to forming a filter cake layer, accompanied by concentration polarization, which causes the membrane flux to drop rapidly. This leads to frequent shutdowns for cleaning or replacement of membrane modules, which not only significantly increases maintenance costs but also seriously affects the continuity of production.

[0008] To achieve the above objectives, the present invention provides the following technical solution: a bioactive peptide separation and purification device, comprising a shell, a controller provided at the bottom of the front side of the shell, a separation component provided inside the shell, and a shaking component provided on both sides of the separation component, the shaking component comprising a rubber connecting block;

[0009] The separation component includes a mounting frame, the interior of which has multiple through slots and a movable frame is disposed inside each slot. An ultrafiltration membrane, a nanofiltration membrane, and a reverse osmosis membrane are fixedly connected inside the movable frame, and the ultrafiltration membrane, nanofiltration membrane, and reverse osmosis membrane are arranged sequentially from top to bottom. A positioning plate is fixedly connected to the outside of the mounting frame, and a magnetic block is fixedly connected to the side of the positioning plate near the mounting frame. A mating block is fixedly connected to the side of the mounting frame near the magnetic block.

[0010] Preferably, vertical grooves are provided on both sides of the housing, and the rubber connecting blocks are fixedly connected to both sides of the mounting frame and movably connected inside the vertical grooves.

[0011] Preferably, a drive motor is fixedly connected to the left side of the mounting frame, a disc is fixedly connected to the output end of the drive motor, a rotating rod is rotatably connected to the surface of the disc, a connecting rod is fixedly connected to the side of the rubber connecting block near the rotating rod, and the end of the rotating rod away from the disc is rotatably connected to the connecting rod.

[0012] Preferably, a guide rod is fixedly connected inside the vertical groove, and the rubber connecting block is slidably connected to the surface of the guide rod.

[0013] Preferably, a delivery pump is fixedly connected to the top of the housing, a suction pipe is fixedly connected to the suction end of the delivery pump, and a conduit is fixedly connected to the discharge pipe of the delivery pump. The conduit passes through the top of the housing and is located directly above the mounting frame.

[0014] Preferably, the bottom of the housing is fixedly connected to a base plate, and the four corners of the bottom of the base plate are fixedly connected to support legs, and the interior of the base plate is fixedly connected to a conical cylinder.

[0015] Preferably, slots are provided on both sides of the inner wall of the mounting frame, and blocks are fixedly connected to both sides of the movable frame, with the blocks cooperating with the slots.

[0016] Preferably, the front side of the housing has a through hole, and an opening and closing door is rotatably connected inside the through hole.

[0017] Compared with the prior art, the beneficial effects of this utility model are:

[0018] 1. This application sets up a separation component, which uses ultrafiltration membrane, nanofiltration membrane and reverse osmosis membrane for graded filtration from coarse to fine. This can avoid the efficiency decline caused by excessive load on a single membrane layer. At the same time, by accurately retaining substances of different molecular weights, it can significantly improve the recovery rate and purity of active peptides. When it needs to be replaced, the movable frame can be directly removed for cleaning or replacement, reducing maintenance costs and downtime.

[0019] 2. By setting up a shaking component, this application can make the separation component generate high-frequency small-amplitude shaking, which can shake off large molecular impurities or colloidal particles adsorbed on the surface of ultrafiltration membrane and nanofiltration membrane, preventing them from accumulating and forming a filter cake layer. For reverse osmosis membrane, it can reduce the crystallization or adsorption of inorganic salts or small molecular organic matter on the membrane surface, avoid membrane pore blockage, and thus maintain stable filtration efficiency. Attached Figure Description

[0020] Figure 1 This is an overall structural diagram of the bioactive peptide separation and purification device of this utility model;

[0021] Figure 2 This utility model Figure 1 A schematic diagram of the opening of a center-hinged door;

[0022] Figure 3 This is a schematic diagram of the structure of the separation component of this utility model;

[0023] Figure 4 This is a schematic diagram of the structure of the vibration component of this utility model;

[0024] Figure 5 This utility model Figure 3 Enlarged diagram of point A in the middle.

[0025] In the diagram, 1. Housing; 2. Controller; 3. Separation assembly; 301. Mounting frame; 302. Movable frame; 303. Ultrafiltration membrane; 304. Nanofiltration membrane; 305. Reverse osmosis membrane; 306. Positioning plate; 4. Vibration assembly; 401. Rubber connecting block; 402. Drive motor; 403. Disc; 404. Rotating rod; 405. Connecting rod; 406. Guide rod; 5. Through groove; 6. Magnetic block; 7. Mating block; 8. Vertical groove; 9. Transfer pump; 10. Suction pipe; 11. Guide tube; 12. Base plate; 13. Support leg; 14. Conical cylinder; 15. Slot; 16. Locking block; 17. Through hole; 18. Opening and closing door. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0027] Please see Figure 1-5 The present invention provides the following technical solution:

[0028] A bioactive peptide separation and purification device includes a shell 1, a controller 2 is provided at the bottom of the front side of the shell 1, a separation component 3 is provided inside the shell 1, and a shaking component 4 is provided on both sides of the separation component 3. The shaking component 4 includes a rubber connecting block 401.

[0029] The separation component 3 includes a mounting frame 301. The mounting frame 301 has multiple through slots 5 inside, and a movable frame 302 is set inside the through slots 5. An ultrafiltration membrane 303, a nanofiltration membrane 304, and a reverse osmosis membrane 305 are fixedly connected inside the movable frame 302, and the ultrafiltration membrane 303, nanofiltration membrane 304, and reverse osmosis membrane 305 are arranged sequentially from top to bottom. A positioning plate 306 is fixedly connected to the outside of the mounting frame 301. A magnetic block 6 is fixedly connected to the side of the positioning plate 306 near the mounting frame 301. A mating block 7 is fixedly connected to the side of the mounting frame 301 near the magnetic block 6.

[0030] In this embodiment: by setting the separation component 3, the mounting frame 301 serves as the carrier for the ultrafiltration membrane 303, nanofiltration membrane 304, and reverse osmosis membrane 305, forming a complete filtration structure. Multiple channels 5 are arranged along the height direction of the mounting frame 301, facilitating the layered arrangement of filter membranes with different precision. The movable frame 302 is an independent encapsulation carrier for the ultrafiltration membrane 303, nanofiltration membrane 304, and reverse osmosis membrane 305, enabling modular replacement. Its edge sealing design prevents sideflow of the mixed solution, ensuring that all mixed solutions must be filtered through the ultrafiltration membrane 303, nanofiltration membrane 304, or reverse osmosis membrane 305. Ultrafiltration membrane 303 can retain large molecular impurities, reducing the fouling load of subsequent nanofiltration membrane 304 and reverse osmosis membrane 305. Nanofiltration membrane 304 can retain some small molecule organic matter and perform preliminary classification of active peptides. Reverse osmosis membrane 305 can retain almost all ions and small molecule organic matter, achieving high purification and concentration of active peptides. Positioning plate 306 can provide a fixed position for magnetic block 6, ensuring precise alignment with housing 1 mating block 7 during installation. Magnetic block 6 can adsorb mating block 7, thereby firmly fixing the mounting frame 301 in the through groove 5, ensuring the overall stability of the separation component 3.

[0031] Specifically, such as Figure 2 , Figure 4 As shown, vertical grooves 8 are provided on both sides of the housing 1. Rubber connecting blocks 401 are fixedly connected to both sides of the mounting frame 301 and movably connected inside the vertical grooves 8.

[0032] Specifically, such as Figure 4 As shown, a drive motor 402 is fixedly connected to the left side of the mounting frame 301. A disc 403 is fixedly connected to the output end of the drive motor 402. A rotating rod 404 is rotatably connected to the surface of the disc 403. A connecting rod 405 is fixedly connected to the side of the rubber connecting block 401 near the rotating rod 404. The end of the rotating rod 404 away from the disc 403 is rotatably connected to the connecting rod 405.

[0033] Specifically, such as Figure 4 As shown, a guide rod 406 is fixedly connected inside the vertical groove 8, and a rubber connecting block 401 is slidably connected to the surface of the guide rod 406.

[0034] In this embodiment: Through the above settings, the vertical groove 8 can provide a vertical track for the rubber connecting block 401, restricting the rubber connecting block 401 to slide only in the vertical direction, ensuring the accuracy of the movement trajectory of the mounting frame 301, and avoiding lateral deviation. The rubber connecting block 401 is the connecting part between the mounting frame 301 and the vertical groove 8, and can bear the weight of the mounting frame 301 and the force during the movement, transmitting the movement of the mounting frame 301 to the vertical groove 8. At the same time, when the rubber connecting block 401 contacts the vertical groove 8, it will not cause damage to the vertical groove 8 or itself. The drive motor 402 can drive the disc 403 to rotate through the output shaft. When the disc 403 rotates, one end of the rotating rod 404 will move in a circular motion with the disc 403. The other end pushes the rubber connecting block 401 to move vertically through the connecting rod 405, thereby converting the circular motion of the disc 403 into the vertical reciprocating motion of the connecting rod 405. In turn, the rubber connecting block 401 drives the mounting frame 301 to move up and down reciprocally, achieving a high-frequency, small-amplitude vibration effect of the separation component 3. The connecting rod 405 is the connecting part between the rubber connecting block 401 and the rotating rod 404, which can ensure the stability of motion transmission and avoid stress concentration or motion jamming caused by rigid connection. The guide rod 406 can provide rigid guidance for the rubber connecting block 401, ensuring that the rubber connecting block 401 can only slide along the axis of the guide rod 406, preventing tilting or deviation during movement, thereby improving stability and reliability.

[0035] Specifically, such as Figure 2 As shown, a delivery pump 9 is fixedly connected to the top of the housing 1. The suction end of the delivery pump 9 is fixedly connected to a suction pipe 10, and the discharge pipe of the delivery pump 9 is fixedly connected to a conduit 11. The conduit 11 passes through the top of the housing 1 and is located directly above the mounting frame 301.

[0036] Specifically, such as Figure 2 As shown, a base plate 12 is fixedly connected to the bottom of the housing 1, and support legs 13 are fixedly connected to the four corners of the bottom of the base plate 12. A conical cylinder 14 is fixedly connected inside the base plate 12.

[0037] In this embodiment: With the above settings, the delivery pump 9 is the power source for transporting the mixture. The pipette 10 can draw the bioactive peptide mixture to be separated from the external container and deliver the mixture to the separation area in the mounting frame 301 through the conduit 11, ensuring that the mixture is evenly distributed or directly acts on the separation component 3. The base plate 12 and the support legs 13 can provide a horizontal support surface for the entire device, dispersing the weight of the device and maintaining stability. The conical cylinder 14 is located at the bottom of the device and can be used to collect the purified bioactive peptide solution. The conical structure allows the solution to naturally converge to the bottom, facilitating subsequent discharge or further processing.

[0038] Specifically, such as Figure 5As shown, slots 15 are provided on both sides of the inner wall of the mounting frame 301, and blocks 16 are fixedly connected to both sides of the movable frame 302. The blocks 16 and the slots 15 are used in conjunction.

[0039] Specifically, such as Figure 2 As shown, a through hole 17 is provided on the front side of the housing 1, and an opening and closing door 18 is rotatably connected inside the through hole 17.

[0040] In this embodiment: Through the above settings, the slot 15 can provide a precise installation position for the movable frame 302, ensuring the stability of the movable frame 302 within the mounting frame 301 and preventing displacement due to vibration or fluid impact during separation. Furthermore, the insertion of the card block 16 into the slot 15 can guide the movable frame 302 to be accurately aligned, reducing installation errors. The through hole 17 can provide an installation base for the opening and closing door 18, forming an openable channel on the front side of the device, facilitating direct contact between the operator and the separation component 3. The opening and closing door 18 is opened and closed through a rotating connection, making it convenient for the operator to remove the movable frame 302 for cleaning, replacement, or maintenance, thereby improving ease of use.

[0041] Working principle: First, the transfer pump 9 is started by the controller 2. The transfer pump 9 draws in the bioactive peptide mixture to be separated through the suction pipe 10, and then delivers it through the conduit 11 to the upper part of the movable frame 302 inside the mounting frame 301. The mixture first contacts the ultrafiltration membrane 303, which traps large molecular impurities. The mixture after preliminary filtration flows into the nanofiltration membrane 304 below, which further traps medium molecular weight impurities. The mixture continues to flow to the reverse osmosis membrane 305, which traps small molecular weight impurities and enriches high-purity bioactive peptides. At the same time, the drive motor 402 is started by the controller 2, which drives the disc 403 to rotate. The rotation of the disc 403 causes the rotating rod 404 to swing. The movement of the rotating rod 404 causes the rubber connecting block 401 to slide up and down along the guide rod 406 within the vertical groove 8, resulting in high-frequency, small-amplitude vibration of the entire separation assembly 3. This vibration prevents the surfaces of the ultrafiltration membrane 303, nanofiltration membrane 304, and reverse osmosis membrane 305 from clogging due to impurity accumulation, promotes turbulence in the mixed liquid, and enhances the separation effect. After multi-stage separation, substances of different molecular weights are retained layer by layer, and the purified mixed liquid is discharged through the conical cylinder 14. When cleaning and maintenance of the separation assembly 3 is required, first open the opening and closing door 18, pull the positioning plate 306 outward to release the adsorption between the magnetic block 6 and the mating block 7, so that the movable frame 302 can be removed from the vertical groove 8, and the retained impurities can be cleaned.

[0042] The above are merely preferred embodiments of the present utility model and are not intended to limit the present utility model. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A device for the isolation and purification of biologically active peptides comprising a housing (1), characterised in that: A controller (2) is provided at the bottom of the front side of the housing (1), a separation component (3) is provided inside the housing (1), and a shaking component (4) is provided on both sides of the separation component (3). The shaking component (4) includes a rubber connecting block (401). The separation component (3) includes a mounting frame (301). The mounting frame (301) has multiple through slots (5) inside and a movable frame (302) is provided inside the through slots (5). An ultrafiltration membrane (303), a nanofiltration membrane (304), and a reverse osmosis membrane (305) are fixedly connected inside the movable frame (302), and the ultrafiltration membrane (303), nanofiltration membrane (304), and reverse osmosis membrane (305) are arranged sequentially from top to bottom. A positioning plate (306) is fixedly connected to the outside of the mounting frame (301). A magnetic block (6) is fixedly connected to the side of the positioning plate (306) near the mounting frame (301). A mating block (7) is fixedly connected to the side of the mounting frame (301) near the magnetic block (6).

2. The apparatus for separating and purifying bioactive peptides according to claim 1, wherein: Vertical grooves (8) are provided on both sides of the housing (1). The rubber connecting block (401) is fixedly connected to both sides of the mounting frame (301) and is movably connected inside the vertical groove (8).

3. The apparatus for separating and purifying bioactive peptides according to claim 1, wherein: A drive motor (402) is fixedly connected to the left side of the mounting frame (301). A disc (403) is fixedly connected to the output end of the drive motor (402). A rotating rod (404) is rotatably connected to the surface of the disc (403). A connecting rod (405) is fixedly connected to the side of the rubber connecting block (401) near the rotating rod (404). The end of the rotating rod (404) away from the disc (403) is rotatably connected to the connecting rod (405).

4. The apparatus of claim 2, wherein: A guide rod (406) is fixedly connected inside the vertical groove (8), and the rubber connecting block (401) is slidably connected to the surface of the guide rod (406).

5. The apparatus of claim 1, wherein: A delivery pump (9) is fixedly connected to the top of the housing (1). The suction end of the delivery pump (9) is fixedly connected to a suction pipe (10). The discharge pipe of the delivery pump (9) is fixedly connected to a conduit (11). The conduit (11) passes through the top of the housing (1) and is located directly above the mounting frame (301).

6. The apparatus of claim 1, wherein: The bottom of the housing (1) is fixedly connected to a base plate (12), and the four corners of the bottom of the base plate (12) are fixedly connected to support legs (13). The inside of the base plate (12) is fixedly connected to a conical cylinder (14).

7. The apparatus of claim 1, wherein: The mounting frame (301) has slots (15) on both sides of its inner wall, and the movable frame (302) has blocks (16) fixedly connected to both sides. The blocks (16) are used in conjunction with the slots (15).

8. The apparatus of claim 1, wherein: The front side of the housing (1) is provided with a through hole (17), and an opening and closing door (18) is rotatably connected inside the through hole (17).

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