An electrochemical detection device for small molecule compounds based on nucleic acid aptamer

Abstract

The utility model discloses a kind of small molecule compound electrochemical detection devices based on nucleic acid aptamer, comprising: sample detection container, including small molecule compound test electrode array, reference correction electrode array and sample tank, small molecule compound test electrode array, reference correction electrode array are placed in sample tank;Controller, the electric control end of small molecule compound test electrode array and reference correction electrode array is connected to controller, correction button and detection button are connected on the controller, detection button controls the switch of small molecule compound test electrode array, correction button controls the switch of reference correction electrode array;And integrated screen, connected to controller, display the detection data of small molecule compound test electrode array and reference correction electrode array.The utility model based on nucleic acid aptamer can simultaneously detect small molecule compound content in the sample to be measured and reference, so as to more accurately evaluate small molecule compound content in sample.

Description

Technical Field

[0001] This invention belongs to the field of electrochemical detection technology, and particularly relates to an electrochemical detection device for small molecule compounds based on nucleic acid aptamers. Background Technology

[0002] To detect small molecule compounds in biological or environmental samples, the main methods currently used include mass spectrometry and chemiluminescence. However, both methods currently have the following limitations:

[0003] First, mass spectrometry has significant limitations in measuring the content of small molecule compounds. Firstly, it places high demands on the instrument. While mass spectrometry is a high-precision detection device, its coverage in sample detection is relatively narrow. Many biological and environmental sample testing institutions do not employ this instrument, thus limiting its application. Secondly, mass spectrometry requires rigorous sample pretreatment processes, which are time-consuming and do not achieve the goal of convenient detection.

[0004] Secondly, chemiluminescence immunoassay is mainly developed based on antigen-antibody reactions of biological macromolecules, and lacks a mature technical system for detecting small molecule compounds. Chemiluminescence immunoassay also has certain limitations. Firstly, it is a multi-step detection method with cumbersome experimental procedures, requiring multiple water bath heating cycles, and a detection time exceeding 2 hours. It is highly dependent on detection instruments and equipment, lacking convenience. Furthermore, the luminescence intensity decays over time during detection, so variations in detection time lead to highly unstable experimental results and poor batch-to-batch repeatability.

[0005] Existing electrochemical (E-AB) sensors using nucleic acid aptamers are an emerging technology, currently having some application in the detection of small molecules in blood. However, this device currently has the following shortcomings:

[0006] First, since existing electrochemical sensors for nucleic acid aptamers mostly detect the concentration of single molecules in blood, they can only detect single molecules in the sample. However, in this invention, in addition to detecting the target small molecule compound in the sample, it also detects its reference as an internal calibration tool. Therefore, this integrated sensor integrates two nucleic acid aptamer detection units.

[0007] Secondly, the existing electrochemical (E-AB) sensor for nucleic acid aptamers, as a handheld detection and screening instrument, is inconvenient for authors who are already wearing gloves in some application environments due to its integrated touch screen operation, and is prone to accidental touches.

[0008] This utility model content

[0009] To overcome the shortcomings of the aforementioned existing technologies, the purpose of this invention is to propose an electrochemical detection device for small molecule compounds based on nucleic acid aptamers. Based on nucleic acid aptamers, this device can simultaneously detect the content of small molecule compounds and reference compounds in a sample. This integrated detection mode can more accurately assess the content of small molecule compounds in a sample.

[0010] To achieve the above objectives, the present invention adopts the following technical solution: an electrochemical detection device for small molecule compounds based on nucleic acid aptamers, comprising:

[0011] The sample detection container includes a small molecule compound test electrode array, a reference calibration electrode array, and a sample slot, with the small molecule compound test electrode array and the reference calibration electrode array placed inside the sample slot;

[0012] The controller connects the electrical control terminals of the small molecule compound test electrode array and the reference calibration electrode array to the controller. The controller is equipped with a calibration button and a detection button. The detection button controls the switching on and off of the small molecule compound test electrode array, and the calibration button controls the switching on and off of the reference calibration electrode array.

[0013] It integrates a screen, which connects to the controller, to display the detection data of the small molecule compound test electrode array and the reference calibration electrode array.

[0014] Furthermore, a sample container lid is provided, which covers the sample slot.

[0015] Furthermore, the device includes a housing on which the sample detection container and integrated screen are disposed, and a control area is also disposed on the housing, the control area including buttons and indicator lights.

[0016] Furthermore, a calibration indicator light and a detection indicator light are also provided. The electrical control terminals of the calibration indicator light and the detection indicator light are connected to the controller. The detection indicator light indicates the on / off status of the small molecule compound test electrode array, and the calibration indicator light indicates the on / off status of the reference calibration electrode array.

[0017] Furthermore, the controller is connected to an instrument switch to control the on / off state of the detection device.

[0018] Furthermore, the controller is connected to an instrument status indicator light, which indicates the on / off status of the detection device.

[0019] Furthermore, the small molecule compound testing electrode array includes a working electrode, a counter electrode, and a pseudo reference electrode arranged in parallel; the working electrode uses a methylene blue portion and a hexyl thiol portion to modify both ends of the nucleic acid aptamer of the small molecule compound, and coats it on a gold surface containing a mercaptohexanol layer.

[0020] Furthermore, the reference calibration electrode array includes a working electrode, a counter electrode, and a spurious reference electrode arranged in parallel; the working electrode uses a methylene blue portion and a hexylthiol portion to modify both ends of the nucleic acid aptamer of the reference, and then coats it on a gold surface containing a mercaptohexanol layer.

[0021] The beneficial effects of adopting this technical solution are:

[0022] This invention adds a reference calibration electrode array, which serves as an internal reference when urine is used as the test sample. Unlike previous single nucleic acid aptamer detection arrays, this device simultaneously detects the content of both the target small molecule compound and the reference molecule in the sample container, incorporating an internal calibration mechanism to accurately reflect the content of the small molecule target.

[0023] This invention adds an instrument control area, allowing direct control of the instrument via indicator lights and push-button switches. The main function buttons and result displays are externalized, making operation simple and clear. Simultaneously, it can separately control the target small molecule compound electrode array and the reference calibration electrode array, and can display the content value of the measured small molecule compound as needed, thus offering greater flexibility and wider application value. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of the structure of an electrochemical detection device for small molecule compounds based on nucleic acid aptamers according to this utility model;

[0025] Figure 2 This is a schematic diagram of the sample detection container in an embodiment of the present invention;

[0026] Figure 3 This is a schematic diagram of the structure of the small molecule compound testing electrode array in the embodiments of this utility model;

[0027] Figure 4 This is a schematic diagram of the reference calibration electrode array in an embodiment of the present invention;

[0028] In this diagram, 1 is the sample detection container, 1-1 is the small molecule compound testing electrode array, 1-2 is the reference calibration electrode array, 1-3 is the sample slot, 1-4 is the sample container lid, 2 is the control area, 2-1 is the calibration button, 2-2 is the detection button, 2-3 is the instrument power switch, 2-4 is the calibration indicator light, 2-5 is the detection indicator light, 2-6 is the instrument status indicator light, and 3 is the integrated screen; 1-1-1 is the working electrode of the small molecule compound testing electrode array, 1-1-2 is the reverse electrode of the small molecule compound testing electrode array, 1-1-3 is the pseudo-reference electrode of the small molecule compound testing electrode array, 1-1-1-1 is the methylene blue portion of the working electrode of the small molecule compound testing electrode array, 1-1-1-2 is the nucleic acid aptamer of the small molecule compound, and 1-1-1-3 is the small molecule... The working electrode of the compound testing electrode array has a hexylthiol portion; 1-1-1-4 is the mercaptohexanol layer of the working electrode of the small molecule compound testing electrode array; 1-1-1-5 is the gold surface of the working electrode of the small molecule compound testing electrode array; 1-2-1 is the working electrode of the reference calibration electrode array; 1-2-2 is the reverse electrode of the reference calibration electrode array; 1-2-3 is the pseudo-reference electrode of the reference calibration electrode array; 1-2-1-1 is the methylene blue portion of the working electrode of the reference calibration electrode array; 1-2-1-2 is the nucleic acid aptamer of the reference; 1-2-1-3 is the hexylthiol portion of the working electrode of the reference calibration electrode array; 1-2-1-4 is the mercaptohexanol layer of the working electrode of the reference calibration electrode array; 1-2-1-5 is the gold surface of the working electrode of the reference calibration electrode array. Detailed Implementation

[0029] To make the purpose, technical solution and advantages of this utility model clearer, the present utility model will be further described below with reference to the accompanying drawings.

[0030] In this embodiment, see Figure 1 As shown, an electrochemical detection device for small molecule compounds based on nucleic acid aptamers includes:

[0031] The sample detection container 1 includes a small molecule compound testing electrode array 1-1, a reference calibration electrode array 1-2, and a sample slot 1-3. The small molecule compound testing electrode array 1-1 and the reference calibration electrode array 1-2 are placed inside the sample slot 1-3. Figure 2 As shown;

[0032] The controller connects the electrical control terminals of the small molecule compound test electrode array 1-1 and the reference calibration electrode array 1-2 to the controller. The controller is equipped with a calibration button 2-1 and a detection button 2-2. The detection button 2-2 controls the switching on and off of the small molecule compound test electrode array 1-1, and the calibration button 2-1 controls the switching on and off of the reference calibration electrode array 1-2.

[0033] The integrated screen 3 is connected to the controller and displays the detection data of the small molecule compound test electrode array 1-1 and the reference calibration electrode array 1-2.

[0034] Creatinine may be used as the reference.

[0035] As an optimization of the above embodiments, such as Figure 2 As shown, a sample container lid 1-4 is also provided, which covers the sample slot 1-3.

[0036] As an optimization of the above embodiments, such as Figure 1 As shown, the device includes a housing, on which the sample detection container 1 and the integrated screen 3 are disposed. A control area 2 is also disposed on the housing, and the control area 2 includes buttons and indicator lights.

[0037] This invention addresses the issue that, as a handheld testing and screening instrument, the traditional method of operation via an integrated touchscreen 3 presents significant inconvenience for workers wearing gloves in certain application environments. Therefore, this invention improves upon this by adding an instrument control area 2, which controls the instrument's operation and displays its status via buttons and indicator lights. The indicator lights and button switches directly control the instrument's operation, while the main function buttons and result displays are externalized, making operation simple and clear.

[0038] The control area 2 is also equipped with an instrument switch 2-3, which is connected to the controller to control the on / off state of the detection device.

[0039] The indicator lights include calibration indicator light 2-4, detection indicator light 2-5, and instrument status indicator light. Detection indicator light 2-5 indicates the on / off status of the small molecule compound test electrode array 1-1, calibration indicator light 2-4 indicates the on / off status of the reference calibration electrode array 1-2, and instrument status indicator light 2-6 indicates the on / off status of the detection device.

[0040] As an optimization of the above embodiments, such as Figure 3 As shown, the small molecule compound testing electrode array 1-1 includes a working electrode 1-1-1, a counter electrode 1-1-2, and a pseudo-reference electrode 1-1-3 arranged in parallel. The working electrode 1-1-1 uses a methylene blue portion 1-1-1-1 and a hexylthiol portion 1-1-1-2 to modify both ends of the nucleic acid aptamer 1-1-1-3 of the small molecule compound, and coats it onto a gold surface 1-1-1-5 containing a mercaptohexanol layer 1-1-1-4. 3'-Methylene blue, 5'-hexylthiol, and 6-mercaptohexanol can be used.

[0041] like Figure 4As shown, the reference calibration electrode array 1-2 includes a working electrode 1-2-1, a counter electrode 1-2-2, and a pseudo-reference electrode 1-2-3 arranged in parallel. The working electrode 1-2-1 uses a methylene blue portion 1-2-1-1 and a hexylthiol portion 1-2-1-2 to modify both ends of the nucleic acid aptamer of the reference, and coats it onto a gold surface 1-2-1-5 containing a mercaptohexanol layer 1-2-1-4. 3'-Methylene blue, 5'-hexylthiol, and 6-mercaptohexanol can be used.

[0042] To better understand this utility model, the working principle of this utility model will be described in detail below:

[0043] The sample container is equipped with a small molecule compound testing electrode array 1-1 and a reference calibration electrode array 1-2, which can simultaneously detect the target small molecule compound and the calibration molecule reference. After adding the sample to the sample slot 1-3 and closing the sample container lid 1-4, the sample is detected by the small molecule compound testing electrode array 1-1 and the reference calibration electrode array 1-2 to obtain the content of small molecule compounds in the calibrated sample.

[0044] Calibration button 2-1 controls the reference calibration electrode array 1-2 to detect the reference content. After detection, calibration indicator light 2-4 illuminates, and the reference content is displayed on the integrated screen 3. Detection button 2-2 controls the small molecule compound detection electrode array to detect the small molecule compound content. After detection, detection indicator light 2-5 illuminates. Instrument switch 2-3 controls the instrument's on / off state. When instrument switch 2-3 is pressed, instrument status indicator light 2-6 illuminates, indicating that the instrument is in good condition and ready for normal use.

[0045] This detection device uses urine as a sample. Creatinine is often used as a calibration tool for detecting small molecule compounds in urine. Therefore, unlike the traditional single working electrode design, this invention adds a creatinine calibration electrode to the electrochemical sensor of nucleic acid aptamers. At the same time, the content of two molecules in urine is measured by nucleic acid aptamers of creatinine and nucleic acid aptamers of small molecule compounds 1-1-1-3. After calibration, the content of small molecule compounds can be accurately reflected.

[0046] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. An electrochemical detection device for small molecule compounds based on nucleic acid aptamer, characterized in that, include: The sample detection container (1) includes a small molecule compound testing electrode array (1-1), a reference calibration electrode array (1-2), and a sample slot (1-3), with the small molecule compound testing electrode array (1-1) and the reference calibration electrode array (1-2) placed in the sample slot (1-3); The controller connects the electrical control terminals of the small molecule compound test electrode array (1-1) and the reference calibration electrode array (1-2). A calibration button (2-1) and a detection button (2-2) are connected to the controller. The detection button (2-2) controls the switching on and off of the small molecule compound test electrode array (1-1), and the calibration button (2-1) controls the switching on and off of the reference calibration electrode array (1-2). The integrated screen (3) is connected to the controller to display the detection data of the small molecule compound test electrode array (1-1) and the reference calibration electrode array (1-2).

2. The device for electrochemical detection of a small molecule compound based on aptamer according to claim 1, wherein, It is also equipped with a sample container lid (1-4), which covers the sample slot (1-3).

3. The aptamer-based small molecule electrochemical detection device according to claim 1, wherein, The device includes a housing on which the sample detection container (1) and an integrated screen (3) are disposed. A control area (2) is also disposed on the housing, the control area (2) including buttons and indicator lights.

4. The aptamer-based small molecule electrochemical detection device according to claim 1 or 3, wherein, It is also equipped with a calibration indicator (2-4) and a detection indicator (2-5). The electrical control terminals of the calibration indicator (2-4) and the detection indicator (2-5) are connected to the controller. The detection indicator (2-5) indicates the on / off status of the small molecule compound test electrode array (1-1), and the calibration indicator (2-4) indicates the on / off status of the reference calibration electrode array (1-2).

5. The aptamer-based small molecule electrochemical detection device according to claim 1 or 3, wherein, The controller is connected to an instrument switch (2-3) to control the on / off state of the detection device.

6. The aptamer-based small molecule electrochemical detection device according to claim 4, wherein, The controller is connected to an instrument status indicator light (2-6), which indicates the on / off status of the detection device.

7. The electrochemical detection device for small molecule compounds based on nucleic acid aptamers according to claim 1, characterized in that, The small molecule compound test electrode array (1-1) includes a working electrode (1-1-1), a counter electrode (1-1-2), and a pseudo reference electrode (1-1-3) arranged in parallel. The working electrode (1-1-1) is modified with methylene blue (1-1-1-1) and hexylthiol (1-1-1-2) portions at both ends of the nucleic acid aptamer (1-1-1-3) of the small molecule compound and coated onto a gold surface (1-1-1-5) containing a mercaptohexanol layer (1-1-1-4).

8. The aptamer-based small molecule electrochemical detection device according to claim 1, wherein, The reference calibration electrode array (1-2) includes a working electrode (1-2-1), a counter electrode (1-2-2), and a pseudo reference electrode (1-2-3) arranged in parallel. The working electrode (1-2-1) is modified at both ends of the nucleic acid aptamer of the reference using a methylene blue portion (1-2-1-1) and a hexylthiol portion (1-2-1-2), and coated on a gold surface (1-2-1-5) containing a mercaptohexanol layer (1-2-1-4).

Images