A reagent cartridge and a sample detection device
By designing a reagent cartridge that includes a housing, reaction tube, and rotary valve, and utilizing a piston to create negative pressure to achieve liquid mixing, the problems of complex structure and high cost of existing devices are solved, and the accuracy of detection results is improved.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- GUANGDONG RUNPENG BIOLOGICAL TECH CO LTD
- Filing Date
- 2025-06-05
- Publication Date
- 2026-07-07
AI Technical Summary
Existing point-of-care molecular detection devices are complex in structure and expensive, and the liquid cannot be automatically mixed in the reaction tube, which affects the accuracy of the detection results.
Design a reagent cartridge including a cartridge body, a reaction tube, a piston chamber, and a rotary valve. The piston moves within the piston chamber to create negative pressure, allowing the liquid from the elution chamber to enter the reaction tube and mix. The rotary valve controls the transfer of liquid between different chambers.
It achieves a simple, autonomous mixing function, improves the accuracy of test results, and reduces production costs.
Smart Images
Figure CN224467788U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of nucleic acid detection technology, and more specifically, to a reagent cartridge and a sample detection device. Background Technology
[0002] Most current testing devices are complex in structure and expensive. Especially in point-of-care testing (POCT), cost control of testing consumable reagent cartridges is crucial to the accessibility of testing. Currently, there are various molecular POCT (Point Of Care Testing) products on the market based on microfluidic chip technology. Most of them achieve liquid transfer between different chambers by creating negative pressure or centrifugation, and then detect the liquid within the reaction chamber.
[0003] However, most current point-of-care molecular diagnostic products have complex structures, and the liquid entering the reaction tube cannot be automatically mixed, which may affect the accuracy of subsequent test results. Utility Model Content
[0004] This invention provides a reagent cartridge and sample detection device that can achieve self-mixing, improving the accuracy of subsequent test results, and has a simple structure.
[0005] The embodiments of this utility model can be implemented as follows:
[0006] An embodiment of this utility model provides a reagent card holder, which includes:
[0007] The box body includes an elution chamber, multiple containment chambers, and at least one piston chamber, wherein the piston chamber, the elution chamber, and the multiple containment chambers are connected in sequence and integrated into one unit;
[0008] At least one reaction tube is connected to the piston chamber; the reaction tube has a reaction chamber; the reaction tube has a first conduit and a second conduit; the first conduit connects the piston chamber and the reaction chamber; the second conduit connects the elution chamber and the reaction chamber;
[0009] At least one piston is movably disposed in the piston chamber, the piston being used to create a negative pressure in the piston chamber and the reaction chamber during movement relative to the piston chamber, thereby allowing the liquid in the elution chamber to enter the reaction tube and be mixed.
[0010] In an alternative embodiment, the end of the first conduit away from the piston chamber is close to the bottom of the reaction tube.
[0011] In an alternative embodiment, the end of the second conduit away from the piston chamber is away from the bottom of the reaction tube.
[0012] In an optional embodiment, when the liquid is located in the reaction chamber, the end of the second conduit away from the piston chamber is above the liquid surface, and the end of the first conduit away from the piston chamber is below the liquid surface.
[0013] In an optional embodiment, there are multiple piston chambers, multiple reaction tubes, and multiple pistons arranged side by side; each of the multiple piston chambers corresponds to one of the multiple reaction tubes; each reaction tube is connected to the elution chamber through a corresponding first conduit; and each piston chamber contains one piston that is movably disposed therein.
[0014] In an optional embodiment, the elution chamber is connected to a plurality of piston chambers via a main flow path; each piston chamber has at least one branch flow path; a portion of the branch flow path is connected to the main flow path; each piston chamber is connected to the second conduit of the corresponding connected reaction tube via a portion of the branch flow path.
[0015] The housing also includes a rotary valve, which is located between the elution chamber and the piston chamber, and is located on the path connecting the main flow path and the multiple branch flow paths; the rotary valve is used to connect or disconnect the elution chamber and the reaction tube during rotation.
[0016] In an optional embodiment, the rotary valve includes a valve chamber, a valve stem, and a rotating component. The valve stem is located inside the valve chamber, and one end of the valve stem away from the valve chamber is connected to the rotating component. A through hole is provided on the valve stem. The rotating component is used to rotate the valve stem, so that the valve stem can rotate relative to the valve chamber, thereby allowing the through hole to connect or block the main flow path and the multiple branch flow paths.
[0017] In an optional embodiment, the valve chamber is provided with a first limiting block and a second limiting block along the rotation path of the rotating member, the first limiting block and the second limiting block being used to limit the swing stroke of the rotating member.
[0018] When the rotating member is located at the position of the first limiting block, the through hole connects the main flow path with the multiple branch flow paths.
[0019] In an optional embodiment, the valve stem includes a first valve stem portion, a second valve stem portion, and a connecting portion. One end of the first valve stem portion is connected to the rotating member, the other end of the first valve stem portion is connected to one end of the second valve stem portion, and the connecting portion is connected to the other end of the second valve stem portion.
[0020] The first valve stem portion includes two intersecting plate-like structures, and the second valve stem portion has the through hole.
[0021] In an optional embodiment, the first valve stem portion is provided with annular protrusions spaced apart along the extension direction of the first valve stem portion. The annular protrusions are used to make close contact with the valve chamber to prevent the first valve stem portion and the second valve stem portion from tilting during rotation.
[0022] An embodiment of this utility model also provides a sample detection device, including a detection component as described in any of the above embodiments, wherein the detection component is connected to the reagent cartridge, and the detection component is used to detect the reagent in the reaction tube.
[0023] The beneficial effects of the reagent cartridge and sample detection device of this utility model embodiment include, for example:
[0024] The reagent cartridge includes a body, at least one reaction tube, and at least one piston. The body comprises an elution chamber, multiple containment chambers, and at least one piston chamber, which are sequentially connected and integrated into a single unit. The reaction tube has a reaction chamber. The body has a first conduit and a second conduit. The first conduit connects the piston chamber and the reaction chamber; the second conduit connects the elution chamber and the reaction chamber. The piston is movably disposed in the piston chamber. As the piston moves relative to the piston chamber, it creates a negative pressure within both the piston chamber and the reaction chamber, allowing the liquid from the elution chamber to enter the reaction tube and mix. By providing the second conduit, the liquid from the elution chamber automatically flows into the reaction chamber when the reaction chamber is under negative pressure. Because the first conduit is connected to the piston chamber, the pressure within the reaction chamber can be changed by repeatedly moving the piston relative to the piston chamber, thus mixing the liquid in the reaction tube. This reagent cartridge has a simple structure and can achieve self-mixing, improving the accuracy of subsequent test results. Attached Figure Description
[0025] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.
[0026] Figure 1 This is a schematic diagram from a first-view perspective of the reagent card holder provided in an embodiment of this utility model;
[0027] Figure 2 This is a schematic diagram of the reagent cartridge for the hidden portion of the reaction tube provided in an embodiment of this utility model;
[0028] Figure 3 This is a schematic diagram from a second perspective of the reagent card holder provided in an embodiment of the present invention;
[0029] Figure 4 This is a schematic diagram from a third-person perspective of the reagent cartridge provided in an embodiment of this utility model;
[0030] Figure 5 This is a schematic diagram of the AA cross-section provided in an embodiment of this utility model;
[0031] Figure 6 This is a schematic diagram of the BB cross-section provided in an embodiment of the present invention;
[0032] Figure 7 This is a schematic diagram of a rotary valve provided in an embodiment of the present invention, excluding the valve chamber.
[0033] Icons: 1000 - Reagent cartridge; 100 - Cartridge body; 110 - Elution chamber; 111 - Main flow path; 120 - Piston chamber; 121 - Branch flow path; 130 - Receptacle chamber; 140 - Reaction tube; 141 - Reaction chamber; 142 - First conduit; 143 - Second conduit; 150 - Piston; 160 - Rotary valve; 161 - Valve chamber; 162 - Valve stem; 1621 - First valve stem section; 16211 - Annular protrusion; 1622 - Second valve stem section; 16221 - Through hole; 1623 - Connecting part; 163 - Rotating component; 164 - First limiting block; 165 - Second limiting block. Detailed Implementation
[0034] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. The components of the embodiments of this utility model described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.
[0035] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.
[0036] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.
[0037] In the description of this utility model, it should be noted that if terms such as "upper," "lower," "inner," or "outer" are used to indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship in which the utility model product is usually placed during use, they are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.
[0038] Furthermore, the terms "first" and "second" are used only to distinguish descriptions and should not be interpreted as indicating or implying relative importance.
[0039] It should be noted that, where there is no conflict, the features in the embodiments of this utility model can be combined with each other.
[0040] Molecular diagnostics refers to the technology of making diagnoses by detecting changes in the structure or expression level of genetic material in a patient's body using molecular biology methods. The basis of molecular diagnostic testing is the analysis of genes and their expression products in the tissue cells, hair, anticoagulated or dried blood, and formaldehyde-fixed or paraffin-embedded tissues of the tested individual. By performing nucleic acid testing at the molecular level, accurate detection can be made even before the onset of disease or before the appearance of symptoms, signs, and biochemical changes. However, most current testing devices are complex in structure and expensive. Especially in point-of-care testing (POCT), cost control of the testing consumable cartridge 1000 is crucial to the accessibility of the product. Currently, there are various molecular POCT (Point Of Care Testing) products on the market based on microfluidic chip technology. Most of them achieve liquid transfer between different chambers by creating negative pressure or centrifugation, and then test the liquid in the reaction chamber. Most current molecular point-of-care testing products also have complex structures, and the liquid entering the reaction tube 140 cannot be effectively mixed, which may affect the accuracy of subsequent test results.
[0041] Based on this, please refer to Figures 1-5 The reagent cartridge 1000 provided in the embodiments of this utility model can effectively solve the aforementioned technical problems. The reagent cartridge 1000 has a simple structure and can achieve self-mixing, improving the accuracy of subsequent test results. The reagent cartridge 1000 is applied to sample testing devices; all devices equipped with the reagent cartridge 1000 have the same functions as described above, and will not be elaborated further here.
[0042] The sample detection device in this embodiment includes a detection component and a reagent cartridge 1000. The detection component is connected to the reagent cartridge 1000 and is used to detect the reagent in the reaction tube 140. Of course, the sample detection device may also include other structures, such as a motion mechanism, depending on the actual application, and is not limited here. To facilitate the integration of the sample detection device, in this embodiment, the piston 150 and rotary valve 160 of the reagent cartridge 1000 are both located above the cartridge body 100. This arrangement allows the sample detection device to easily integrate by having a motion mechanism above the reagent cartridge 1000 to drive the piston 150 and rotary valve 160.
[0043] The structure of the reagent cartridge 1000 will be described in detail below.
[0044] Figure 1 This is a schematic diagram from a first-view perspective of the reagent card holder 1000 provided in an embodiment of this utility model; Figure 2 This is a schematic diagram of the reagent card box 1000 of the hidden part reaction tube 140 provided in an embodiment of the present invention; Figure 3 This is a schematic diagram from a second perspective of the reagent card holder 1000 provided in an embodiment of the present invention; Figure 4 This is a schematic diagram from a third-view perspective of the reagent card holder 1000 provided in an embodiment of this utility model; Figure 5 This is a schematic diagram of the AA cross-section provided in an embodiment of this utility model.
[0045] Please see Figures 1-4 and combined Figure 5In this embodiment, the reagent cartridge 1000 includes a cartridge body 100, at least one reaction tube 140, and at least one piston 150. The cartridge body 100 includes an elution chamber 110, multiple containment chambers 130, and at least one piston chamber 120. The piston chamber 120, the elution chamber 110, and the multiple containment chambers 130 are connected in sequence and integrated into one unit. The reaction tube 140 has a reaction chamber 141. The cartridge body 100 has a first conduit 142 and a second conduit 143. The first conduit 142 connects the piston chamber 120 and the reaction chamber 141. The second conduit 143 connects the elution chamber 110 and the reaction chamber 141. The piston 150 is movably disposed in the piston chamber 120. The piston 150 is used to create a negative pressure in the piston chamber 120 and the reaction chamber 141 during the movement of the piston chamber 120 relative to the piston chamber 120, thereby allowing the liquid in the elution chamber 110 to enter the reaction tube 140 and be mixed. By providing a second conduit 143, the liquid in the elution chamber 110 can automatically flow into the reaction chamber 141 when it is under negative pressure. By providing a first conduit 142, which is connected to the piston chamber 120, the pressure inside the reaction chamber 141 can be changed by repeatedly moving the piston 150 relative to the piston chamber 120, thereby mixing the liquid inside the reaction tube 140. In other words, by repeatedly drawing in and out with the piston 150, the pressure inside the reaction chamber 141 is changed, causing the liquid to slosh and thus achieving mixing, ensuring the accuracy of subsequent detection results. This reagent cartridge 1000 has a simple structure and can achieve self-mixing, improving the accuracy of subsequent detection results.
[0046] In this embodiment, the multiple accommodating chambers 130 include a magnetic rod sleeve placement chamber, a sample chamber, a magnetic bead chamber, and at least one cleaning chamber. These chambers are arranged sequentially from the furthest point from the elution chamber 110 to the closest point. The magnetic rod sleeve placement chamber is used to hold the magnetic rod sleeve, which is used to cover the magnetic rod during nucleic acid extraction to prevent contamination. The magnetic rod sleeve placement chamber is divided into a lower fine-pore section and an upper coarse-pore section, which correspond to the upper and lower sections of the magnetic rod sleeve. Specifically, the diameter of the fine-pore section of the magnetic rod sleeve placement chamber is larger than the diameter of the lower section but smaller than the diameter of the upper section, allowing the protrusions of the magnetic rod sleeve to be supported and fixed by the protrusions of the placement chamber, facilitating the pressure connection of the magnetic rod sleeve connecting mechanism to the sleeve section. The sample compartment is used to hold the sample solvent, the magnetic bead compartment is used to hold the magnetic bead preservation solution containing magnetic beads, the washing compartment is used to hold the washing solution, and the elution compartment 110 is used to hold the elution solution. The number of elution compartments 110 can be one or more, and is not limited here.
[0047] Please continue reading. Figures 1-4 and combined Figure 5In this embodiment, the end of the first conduit 142 away from the piston chamber 120 is close to the bottom of the reaction tube 140. By positioning the end of the first conduit 142 extending into the reaction chamber 141 close to the bottom of the reaction tube 140, i.e., close to the liquid surface after liquid is filled into the reaction tube 140, the liquid can be rapidly agitated when the pressure changes, thus ensuring thorough and uniform mixing. Furthermore, to prevent liquid in the reaction tube 140 from flowing back into the elution chamber 110, the end of the second conduit 143 away from the piston chamber 120 in this embodiment is also away from the bottom of the reaction tube 140. That is, the end face of the second conduit 143 extending into the reaction chamber 141 is higher than the liquid surface after liquid is filled into the reaction tube 140, to prevent liquid backflow. Specifically, when the liquid is located in the reaction chamber 141, the end of the second conduit 143 away from the piston chamber 120 is above the liquid surface, and the end of the first conduit 142 away from the piston chamber 120 is below the liquid surface. This design allows the liquid to pass through the first conduit 142 and be repeatedly drawn in by the piston 150 to achieve mixing, and the liquid will not flow back from the second conduit 143 to the elution chamber 110.
[0048] Furthermore, in this embodiment, the length of the first conduit 142 extending into the reaction chamber 141 is longer than the length of the second conduit 143 extending into the reaction chamber 141. Of course, the length of the first conduit 142 extending into the reaction chamber 141 may also be less than or equal to the length of the second conduit 143 extending into the reaction chamber 141, and this is not limited here.
[0049] For multi-tube, multi-parameter testing of liquid samples, please refer to [link / reference]. Figure 1 and Figure 4 In this embodiment, there are multiple piston chambers 120, reaction tubes 140, and pistons 150, with multiple piston chambers 120 arranged side by side; each piston chamber 120 corresponds one-to-one with a multiple reaction tube 140; each reaction tube 140 is connected to the elution chamber 110 via a corresponding first conduit 142; and each piston chamber 120 contains a corresponding movable piston 150. The sample liquid in the elution chamber 110 can flow into multiple reaction tubes 140, allowing the operator to perform multiple tests on the same sample for the same indicator to eliminate errors and improve test results. This arrangement allows the same sample liquid to be divided into multiple portions simultaneously, enabling the operator to perform tests on the same sample for different indicators without requiring multiple sampling, thus saving testing time. In this embodiment, there are two piston chambers 120, two reaction tubes 140, and two pistons 150. However, the number of piston chambers 120 can also be three, four, five, or more, and correspondingly, the number of reaction tubes 140 and pistons 150 can also be three, four, five, or more. The number of piston chamber 120, reaction tube 140 and piston 150 is determined according to the actual testing situation and is not limited here.
[0050] Furthermore, to facilitate convenient and accurate control of the liquid flow channels, thereby enabling liquid transfer between different chambers, and to simplify the structure of the reagent cartridge 1000 and reduce production costs, please refer to [link to relevant documentation]. Figures 1-4 and combined Figure 6 , Figure 6 This is a schematic diagram of the BB cross-section provided in an embodiment of the present invention. In this embodiment, the elution chamber 110 is connected to multiple piston chambers 120 via a main flow path 111; each piston chamber 120 has at least one branch flow path 121; a portion of the branch flow path 121 connects to the main flow path 111; and a portion of each piston chamber 120 is connected to the second conduit 143 of the corresponding reaction tube 140 via the branch flow path 121. The housing 100 also includes a rotary valve 160, which is disposed between the elution chamber 110 and the piston chambers 120, and is located on the path connecting the main flow path 111 and the multiple branch flow paths 121; the rotary valve 160 is used to connect or disconnect the elution chamber 110 and the reaction tube 140 during rotation. By providing the rotary valve 160, the connection and disconnection between the elution chamber 110 and the multiple reaction tubes 140 can be conveniently and accurately controlled, ensuring that the sample liquid can be transferred between different chambers while simplifying the transfer structure and reducing costs.
[0051] Specifically, Figure 7 This is a schematic diagram of the rotary valve 160 provided in an embodiment of the present invention, excluding the valve chamber 161. Please refer to... Figures 1-5 and combined Figure 7In this embodiment, the rotary valve 160 includes a valve chamber 161, a valve stem 162, and a rotating component 163. The valve stem 162 is located inside the valve chamber 161, and one end of the valve stem 162 away from the valve chamber 161 is connected to the rotating component 163. A through hole 16221 is provided on the valve stem 162. The rotating component 163 is used to rotate the valve stem 162, so that the valve stem 162 can rotate relative to the valve chamber, thereby allowing the through hole 16221 to connect or block the main flow path 111 and multiple branch flow paths 121. The openings of the valve chamber 161, piston chamber 120, elution chamber 110, and multiple receiving chambers 130 are all located on the upper end face of the housing 100 for integration of the sample detection device. To facilitate the rotation of the rotating component 163, in this embodiment, the rotating component 163 includes a body and a cuboid protrusion. Rotating the rotating component 163 causes it to rotate, thereby driving the valve stem 162 to rotate relative to the valve chamber 161. The rotating component 163 drives the valve stem 162 to rotate relative to the valve chamber 161, connecting the through hole 16221 to the main flow path 111 and multiple branch flow paths 121. At this time, the piston 150 is driven to move upward relative to the piston chamber 120. The reaction tube 140 is connected to the piston chamber 120 through the first conduit 142, creating a negative pressure in the reaction chamber 141. This allows the liquid in the elution chamber 110 to enter each reaction tube 140 through the main flow path 111, the through hole 16221 of the rotary valve 160, and the multiple branch flow paths 121. The shape of the protrusion is not limited, as long as it facilitates the rotation of the rotating component 163.
[0052] In order to control the rotation angle of the rotary valve 160, and to enable the rotary valve 160 to connect the main flow path 111 and multiple branch flow paths 121 without precise control of the rotation angle, please refer to [reference needed]. Figure 1 and Figure 3 A first limiting block 164 and a second limiting block 165 are provided on the rotation path of the rotating member 163 in the valve chamber 161. The first limiting block 164 and the second limiting block 165 are used to limit the swing stroke of the rotating member 163. When the rotating member 163 is in the position of the first limiting block 164, the through hole 16221 connects the main flow path 111 with multiple branch flow paths 121. Specifically, when the rotating member 163 rotates to the first limiting block 164, the rotary valve 160 is in the first position; when the rotating member 163 is between the first limiting block 164 and the second limiting block 165 and is not in contact with the first limiting block 164, the rotary valve 160 is in the second position. When the rotary valve 160 is in the first position, the through hole 16221 connects the main flow path 111 and the multiple flow paths; when the rotary valve 160 is in the second position, the rotary valve 160 blocks the main flow path 111 from the multiple branch flow paths 121.
[0053] Please continue reading. Figure 7To save materials and reduce costs, the valve stem 162 provided in this embodiment of the present invention includes a first valve stem portion 1621, a second valve stem portion 1622, and a connecting portion 1623. One end of the first valve stem portion 1621 is connected to the swing member, and the other end of the first valve stem portion 1621 is connected to one end of the second valve stem portion 1622. The connecting portion 1623 is connected to the other end of the second valve stem portion 1622. The first valve stem portion 1621 includes two cross-shaped plate structures, and the second valve stem portion 1622 has a through hole 16221. To prevent leakage, the second valve stem portion 1622 is provided with an annular groove, and an annular sealing gasket is embedded in the annular groove. To prevent the first valve stem portion 1621 and the second valve stem portion 1622 from tilting during rotation, in this embodiment, the first valve stem portion 1621 is provided with annular protrusions 16211 at intervals along the extending direction of the first valve stem portion 1621. The annular protrusions 16211 are used to make close contact with the valve chamber 161 to prevent the first valve stem portion 1621 and the second valve stem portion 1622 from tilting during rotation.
[0054] To facilitate the installation of the rotary valve 160 and limit its vertical position (see Figures 1 and 2), the connecting portion 1623 provided in this embodiment includes multiple claws. For each claw, one end is connected to the second valve stem 1622, and the other end has a limiting protrusion. The multiple claws are evenly distributed along the circumference of the second valve stem 162, with gaps between adjacent claws. All claws are made of elastic material and can retract inwards. A protrusion is provided inside the valve chamber 161. When the limiting protrusion passes its position, the multiple claws retract inwards; when the limiting protrusion passes its position, the multiple claws spring back, thereby fixing and limiting the valve stem 162 to the valve chamber 161, thus enabling the installation of the rotary valve 160. The protrusion on the valve chamber 161 can engage the connecting portion 1623, ensuring that the through hole 16221 is on the same horizontal plane as the first and second flow paths, facilitating installation. To facilitate the installation of the valve body into the valve chamber 161, the end of the retainer claw furthest from the second valve stem 1622 is a guide arc surface. In this embodiment, there are four retainers, which are evenly distributed along the circumference of the second valve stem 162, with a gap between adjacent retainers. Of course, the number of retainers can also be two, three, etc., and is not limited here.
[0055] The working principle of the reagent cartridge 1000 provided in this embodiment is as follows:
[0056] Before testing, the sample liquid is injected into the sample chamber. The magnetic rod sleeve grasping mechanism pulls the magnetic rod sleeve assembly out of the magnetic rod sleeve chamber and moves it above the magnetic bead chamber. Then, the motion mechanism drives the magnetic rod sleeve assembly to insert into the magnetic bead chamber to adsorb magnetic beads. After adsorbing the magnetic beads in the magnetic bead chamber, the magnetic rod sleeve assembly sequentially enters the sample chamber, washing chamber, and elution chamber 110 from the magnetic bead chamber. Then, the motion mechanism drives the actuating component to actuate the rotating component 163 of the rotary valve 160, so that the through hole 16221 of the rotary valve 160 connects the main flow path 111 and multiple branch flow paths 121. At this time, the moving component drives the piston 150 to move upward relative to the piston chamber 120, creating a negative pressure in the piston chamber 120. This allows the liquid in the elution chamber 110 to flow into the corresponding reaction tube 140 through the main flow path 111, the through hole 16221 of the rotary valve 160, and the multiple branch flow paths 121. Finally, the piston 150 is driven to move up and down repeatedly relative to the piston chamber 120 to achieve uniform mixing of the sample liquid in the reaction tube 140.
[0057] In summary, the reagent cartridge 1000 includes a cartridge body 100, at least one reaction tube 140, and at least one piston 150. The cartridge body 100 includes an elution chamber 110, multiple containment chambers 130, and at least one piston chamber 120. The piston chamber 120, the elution chamber 110, and the multiple containment chambers 130 are sequentially connected and integrated into one unit. The reaction tube 140 has a reaction chamber 141. The cartridge body 100 has a first conduit 142 and a second conduit 143. The first conduit 142 connects the piston chamber 120 and the reaction chamber 141. The second conduit 143 connects the elution chamber 110 and the reaction chamber 141. The piston 150 is movably disposed in the piston chamber 120. The piston 150 is used to create a negative pressure in the piston chamber 120 and the reaction chamber 141 during the movement of the piston 150 relative to the piston chamber 120, thereby allowing the liquid in the elution chamber 110 to enter the reaction tube 140 and be mixed. By providing a second conduit 143, the liquid in the elution chamber 110 can automatically flow into the reaction chamber 141 when it is under negative pressure. By providing a first conduit 142, which is connected to the piston chamber 120, the pressure inside the reaction chamber 141 can be changed by repeatedly moving the piston 150 relative to the piston chamber 120, thereby mixing the liquid in the reaction tube 140. This reagent cartridge 1000 has a simple structure and can achieve self-mixing, improving the accuracy of subsequent test results.
[0058] The above description is only a specific embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this utility model should be included within the protection scope of this utility model.
Claims
1. A reagent cartridge, characterized by, include: The box body (100) includes a washing chamber (110), a plurality of receiving chambers (130) and at least one piston chamber (120), wherein the piston chamber (120), the washing chamber (110) and the plurality of receiving chambers (130) are connected in sequence and integrated into one unit; At least one reaction tube (140) is connected to the piston chamber (120); the reaction tube (140) has a reaction chamber (141); the housing (100) has a first conduit (142) and a second conduit (143); the first conduit (142) connects the piston chamber (120) and the reaction chamber (141); the second conduit (143) connects the elution chamber (110) and the reaction chamber (141); At least one piston (150) is movably disposed in the piston chamber (120), the piston (150) being used to create a negative pressure in the piston chamber (120) and the reaction chamber (141) during movement relative to the piston chamber (120), thereby allowing the liquid in the elution chamber (110) to enter the reaction tube (140) and be mixed.
2. The reagent cartridge of claim 1, wherein, The end of the first conduit (142) away from the piston chamber (120) is close to the bottom of the reaction tube (140).
3. The reagent cartridge of claim 2, wherein, The end of the second conduit (143) away from the piston chamber (120) is away from the bottom of the reaction tube (140).
4. The reagent cartridge of claim 1, wherein, With the liquid in the reaction chamber (141), the end of the second conduit (143) away from the piston chamber (120) is above the liquid surface, and the end of the first conduit (142) away from the piston chamber (120) is below the liquid surface.
5. The reagent cartridge according to claim 1, characterized in that, The number of piston chambers (120), reaction tubes (140), and pistons (150) are all multiple, with multiple piston chambers (120) arranged side by side; each of the multiple piston chambers (120) is arranged in a one-to-one correspondence with a multiple of the reaction tubes (140); each reaction tube (140) is connected to the elution chamber (110) through a corresponding first conduit (142); and each piston chamber (120) is movably provided with a corresponding piston (150).
6. The reagent cartridge according to claim 5, characterized in that, The elution chamber (110) is connected to a plurality of piston chambers (120) via a main flow path (111); each piston chamber (120) has at least one branch flow path (121); a portion of the branch flow path (121) is connected to the main flow path (111); each piston chamber (120) is connected to the second conduit (143) of the corresponding reaction tube (140) via a portion of the branch flow path (121); The housing (100) also includes a rotary valve (160), which is disposed between the elution chamber (110) and the piston chamber (120), and is disposed on the path connecting the main flow path (111) and the multiple branch flow paths (121); the rotary valve (160) is used to connect or block the elution chamber (110) and the reaction tube (140) during rotation.
7. The reagent cartridge according to claim 6, characterized in that, The rotary valve (160) includes a valve chamber (161), a valve stem (162), and a rotating component (163). The valve stem (162) is located inside the valve chamber (161), and one end of the valve stem (162) away from the valve chamber (161) is connected to the rotating component (163). A through hole (16221) is provided on the valve stem (162). The rotating component (163) is used to rotate the valve stem (162), so that the valve stem (162) can rotate relative to the valve chamber (161), thereby allowing the through hole (16221) to connect or block the main flow path (111) and the multiple branch flow paths (121).
8. The reagent cartridge according to claim 7, characterized in that, The valve chamber (161) is provided with a first limiting block (164) and a second limiting block (165) along the rotation path of the rotating member (163). The first limiting block (164) and the second limiting block (165) are used to limit the swing stroke of the rotating member (163). When the rotating member (163) is in the position of the first limiting block (164), the through hole (16221) connects the main flow path (111) with the multiple branch flow paths (121).
9. The reagent cartridge according to claim 7, characterized in that, The valve stem (162) includes a first valve stem portion (1621), a second valve stem portion (1622), and a connecting portion (1623). One end of the first valve stem portion (1621) is connected to the rotating member (163), the other end of the first valve stem portion (1621) is connected to one end of the second valve stem portion (1622), and the connecting portion (1623) is connected to the other end of the second valve stem portion (1622). The first valve stem portion (1621) includes two cross-shaped plate structures, and the second valve stem portion (1622) has the through hole (16221).
10. The reagent cartridge according to claim 9, characterized in that, The first valve stem portion (1621) is provided with annular protrusions (16211) spaced apart along the extending direction of the first valve stem portion (1621). The annular protrusions (16211) are used to make close contact with the valve chamber (161) to prevent the first valve stem portion (1621) and the second valve stem portion (1622) from tilting during rotation.
11. A sample detection device, characterized in that, The device includes a detection component and a reagent cartridge (1000) as described in any one of claims 1-10, wherein the detection component is connected to the reagent cartridge (1000) and the detection component is used to detect the reagent in the reaction tube (140).