An nk cell engineering blender

Abstract

The utility model relates to the field of bioengineering technology discloses a NK cell modification stirrer, including the container, the top fixedly connected with the fixed plate of container, the top fixedly connected with motor of fixed plate, the output fixedly connected with bevel gear of motor, the outer wall of bevel gear is engaged to have the gear wheel, the outer wall fixedly connected with rotating shaft no.

Description

Technical Field

[0001] This utility model relates to the field of bioengineering technology, and in particular to an NK cell modification stirrer. Background Technology

[0002] NK cells, or natural killer cells, are the core warriors of the body's innate immune system. They are named for their ability to quickly identify and eliminate abnormal cells without prior sensitization. Like patrolmen of the immune system, they can precisely target cancer cells, cells infected by viruses or bacteria, and directly destroy the cell membrane of target cells by releasing perforin and granzyme cytotoxic substances, or regulate the immune response by secreting cytokines such as interferon, thus building the first line of immune defense for the body.

[0003] NK cell modification stirrers are specialized stirring devices used in the field of bioengineering for the in vitro modification and culture of NK cells. Their core function is to provide a stable suspension culture environment for cells, while also achieving efficient mixing in conjunction with gene editing and protein modification processes. However, ordinary stirrers cause the nutrient substrate in the nutrient solution to settle to the bottom of the container, and the stirrer rubs against the bottom of the container, which cannot provide the optimal microenvironment. Furthermore, the friction between the stirrer and the bottom of the container may contaminate the nutrient solution, causing the modification to fail. Utility Model Content

[0004] To overcome the above shortcomings, this invention provides an NK cell-modified stirrer, which aims to improve the problem of friction between the stirrer and the bottom of the container and the precipitation of nutrient substrate at the bottom of the container in the prior art.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: 1. An NK cell-modified stirrer, comprising a container, characterized in that: a fixed plate is fixedly connected to the top of the container, a motor is fixedly connected to the top of the fixed plate, a helical gear is fixedly connected to the output end of the motor, a spur gear is meshed with the outer wall of the helical gear, a rotating shaft one is fixedly connected to the inner wall of the spur gear, a rotating shaft two is meshed with the left and right sides of the rotating shaft one, a paddle is fixedly connected to the outer wall of the rotating shaft two, a waterproof box is rotatably connected to the outer wall of the rotating shaft two, a rotating bearing is fixedly connected to the inner wall of the waterproof box, a water inlet is provided on the front side of the fixed plate, and a temperature control mechanism is fixedly connected to the outer wall of the container, the temperature control mechanism being used to control the temperature.

[0006] As a further description of the above technical solution:

[0007] The temperature control mechanism includes a heat dissipation pipe, the outer wall of which is fixedly connected to the outer wall of the container. A condenser pipe is fixedly connected to the outer wall of the container. An insulation shell is slidably connected to the outer wall of the heat dissipation pipe. An equipment shell is fixedly connected to the outer wall of the insulation shell. A refrigerator is fixedly connected to the inner wall of the equipment shell. A compressor is fixedly connected to the inner wall of the equipment shell. A connecting pipe is connected to the output end of the compressor. A booster pump is fixedly connected to the outer wall of the heat dissipation pipe.

[0008] As a further description of the above technical solution:

[0009] The bottom of the container is fixedly connected to a load-bearing plate, and the bottom of the load-bearing plate is fixedly connected to a support column.

[0010] As a further description of the above technical solution:

[0011] The bottom of the refrigeration unit is fixedly connected to a fixed column, and the bottom of the compressor is fixedly connected to a fixed column.

[0012] As a further description of the above technical solution:

[0013] The top of the container is fixedly connected to a top cover, and the inner wall of the container is fixedly connected to a discharge pipe.

[0014] As a further description of the above technical solution:

[0015] An insulation layer is fixedly connected to the outer wall of the heat dissipation pipe, and an insulation layer is fixedly connected to the outer wall of the condenser pipe.

[0016] As a further description of the above technical solution:

[0017] A valve is rotatably connected to the outer wall of the discharge pipe, and a water pump is fixedly connected to the outer wall of the discharge pipe.

[0018] As a further description of the above technical solution:

[0019] The outer wall of the top cover is rotatably connected to a flip cover, and the top of the flip cover is fixedly connected to a handle.

[0020] This utility model has the following beneficial effects:

[0021] 1. In this utility model, cells and culture medium are poured into the container through the water inlet. Then, the motor is turned on to drive the rotating shaft to rotate the other gears, which in turn rotate the paddle to stir the liquid. The fixed plate prevents the rotating shaft from rubbing against the bottom of the container and causing contamination. This achieves the effect of gently stirring the liquid and preventing the liquid from sinking to the bottom and causing contamination.

[0022] 2. In this utility model, a condenser tube and a heat dissipation tube are wound around the outer wall of the container and connected to a cooler. The heat dissipation tube is connected to a compressor. If the temperature inside the equipment is too high, the cooler will lower the temperature of the coolant in the condenser tube to cool the equipment. If the temperature is too low, the compressor will be turned on to raise the temperature of the heat transfer oil in the heat dissipation tube to raise the temperature of the equipment. This achieves the effect of controlling the temperature of the equipment within a certain range. Attached Figure Description

[0023] Figure 1 This is a three-dimensional view of the front side of an NK cell-modified stirrer proposed in this utility model;

[0024] Figure 2 This is a partial structural diagram of the condenser tube of an NK cell-modified stirrer proposed in this utility model;

[0025] Figure 3 This is a partial top view of the fixing plate of an NK cell modified stirrer proposed in this utility model;

[0026] Figure 4 This is a partial structural diagram of the rotating shaft of an NK cell-modified stirrer proposed in this utility model;

[0027] Figure 5 This is a partial structural breakdown diagram of the rotating shaft of an NK cell modified stirrer proposed in this utility model.

[0028] Legend:

[0029] 1. Container; 2. Temperature control mechanism; 201. Heat dissipation pipe; 202. Condenser pipe; 203. Connecting pipe; 204. Refrigerator; 205. Compressor; 206. Equipment shell; 207. Lift pump; 208. Insulated shell; 3. Motor; 4. Helical gear; 5. Circular gear; 6. Rotating shaft one; 7. Rotating shaft two; 8. Paddle; 9. Water inlet; 10. Waterproof box; 11. Rotating bearing; 12. Fixing plate; 13. Top cover; 14. Flip cover; 15. Insulation layer; 16. Load-bearing plate; 17. Support column; 18. Fixing column; 19. Water pump; 20. Discharge pipe; 21. Valve; 22. Handle. Detailed Implementation

[0030] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0031] Please see the appendix Figure 3 Appendix Figure 4 and attached Figure 5 An embodiment of this utility model is provided: an NK cell modified stirrer, including a container 1, a fixed plate 12 fixedly connected to the top of the container 1, a motor 3 fixedly connected to the top of the fixed plate 12, a helical gear 4 fixedly connected to the output end of the motor 3, a spur gear 5 meshing with the outer wall of the helical gear 4, a rotating shaft 6 fixedly connected to the inner wall of the spur gear 5, a rotating shaft 7 meshing with the left and right sides of the rotating shaft 6, a paddle 8 fixedly connected to the outer wall of the rotating shaft 7, a waterproof box 10 rotatably connected to the outer wall of the rotating shaft 7, a rotating bearing 11 fixedly connected to the inner wall of the waterproof box 10, a water inlet 9 opened on the front side of the fixed plate 12, and a temperature control mechanism 2 fixedly connected to the outer wall of the container 1, the temperature control mechanism 2 being used to control the temperature;

[0032] Specifically, a fixed plate 12 is firmly connected to the upper opening of container 1. A motor 3 is fixedly installed on the top plane of the fixed plate 12 by bolts. A helical gear 4 is mounted on the output shaft of the motor 3. The helical gear 4 meshes perfectly with the external teeth of the spur gear 5. The inner hole of the spur gear 5 is fixedly connected to the rotating shaft 6. The two sides of the rotating shaft 6 are respectively connected to two rotating shafts 7 to form a meshing transmission relationship. Multiple paddles 8 are evenly distributed on the outer surface of each rotating shaft 7. These paddles 8 are arranged in a fan shape and can effectively agitate the liquid. The middle section of the rotating shaft 7 is rotatably connected to the waterproof box 10 through a waterproof rotating bearing 11. The rotating bearing 11 is installed in the inner cavity of the waterproof box 10 to ensure the smooth operation of the transmission system. A water inlet 9 is opened at the front end of the fixed plate 12. A temperature control mechanism 2 is integrated on the outer wall of container 1. The temperature control mechanism 2 can accurately adjust and maintain the internal temperature of container 1 within the set range.

[0033] Please see the appendix Figure 1 Appendix Figure 2 The temperature control mechanism 2 includes a heat dissipation pipe 201. The outer wall of the heat dissipation pipe 201 is fixedly connected to the outer wall of the container 1. A condenser pipe 202 is fixedly connected to the outer wall of the container 1. An insulation shell 208 is slidably connected to the outer wall of the heat dissipation pipe 201. An equipment shell 206 is fixedly connected to the outer wall of the insulation shell 208. A refrigerator 204 is fixedly connected to the inner wall of the equipment shell 206. A compressor 205 is fixedly connected to the inner wall of the equipment shell 206. A connecting pipe 203 is connected to the output end of the compressor 205. A booster pump 207 is fixedly connected to the outer wall of the heat dissipation pipe 201.

[0034] Specifically, the temperature control mechanism 2 mainly consists of a heat dissipation pipe 201, which is firmly connected to the outer wall of the container 1 by welding. A condenser pipe 202 is also installed on the outside of the container 1, and is similarly fixedly connected to the container 1. The heat dissipation pipe 201 is designed with a sliding heat-insulating shell 208, which effectively reduces heat loss. An equipment housing 206 is fixedly installed on the outside of the heat-insulating shell 208. The equipment housing 206 integrates a refrigeration system, including a high-efficiency cooler 204 and a compressor 205 fixed to the inner wall of the equipment housing 206. The compressor 205 is connected to a connecting pipe 203 through its output end, which delivers heat transfer oil to the heat dissipation system. Furthermore, a booster pump 207 is fixedly installed on the outer wall of the heat dissipation pipe 201. This booster pump 207 promotes the circulation of coolant, ensuring the efficient operation of the entire temperature control system. All components of the temperature control mechanism 2 are tightly integrated, forming a complete temperature regulation system.

[0035] Please see the appendix Figure 1 Appendix Figure 2 The bottom of container 1 is fixedly connected to a load-bearing plate 16, the bottom of the load-bearing plate 16 is fixedly connected to a support column 17, the bottom of the cooler 204 is fixedly connected to a fixing column 18, the bottom of the compressor 205 is fixedly connected to a fixing column 18, the top of container 1 is fixedly connected to a top cover 13, and the inner wall of container 1 is fixedly connected to a discharge pipe 20.

[0036] Specifically, a load-bearing plate 16 is firmly fixed to the bottom of container 1. Four support columns 17 are evenly distributed and fixed to the bottom of the load-bearing plate 16. These support columns 17 are vertically installed to ensure the stability of the overall structure. Inside the equipment, two symmetrically distributed fixed columns 18 are fixed to the bottom of the refrigerator 204 by bolts. Similarly, the bottom of the compressor 205 is also supported by the same fixed columns 18. A circular top cover 13 is fixedly connected to the top edge of container 1. A sealing gasket is provided between the top cover 13 and container 1 to ensure airtightness. In addition, a discharge pipe 20 is horizontally fixed to the inner wall of container 1 near the bottom. One end of the discharge pipe 20 extends to the outside of container 1 to facilitate material output.

[0037] Please see the appendix Figure 1 Appendix Figure 2 and attached Figure 4 The outer wall of the heat dissipation pipe 201 is fixedly connected to the heat insulation layer 15, the outer wall of the condenser pipe 202 is fixedly connected to the heat insulation layer 15, the outer wall of the discharge pipe 20 is rotatably connected to the valve 21, the outer wall of the discharge pipe 20 is fixedly connected to the water pump 19, the outer wall of the top cover 13 is rotatably connected to the flip cover 14, and the top of the flip cover 14 is fixedly connected to the handle 22.

[0038] Specifically, a heat insulation layer 15 is firmly fixed to the outer wall of the heat dissipation pipe 201, which can effectively reduce heat loss. Similarly, the outer wall of the condenser pipe 202 is also fixedly connected with a heat insulation layer 15 of the same material using the same process to ensure consistent heat insulation effect. An adjustable flow valve 21 is rotatably connected to the outer wall of the discharge pipe 20, which facilitates precise control of material discharge. At the same time, a water pump 19 is also fixedly installed on the outer wall of the discharge pipe 20. An openable flip cover 14 is rotatably connected to the outer wall of the top cover 13. An ergonomically designed handle 22 is firmly fixed to the top of the flip cover 14. The surface of the handle 22 is treated with anti-slip material for easy operation by the user.

[0039] Working principle: When using the equipment, cells and culture medium are poured into container 1 through water inlet 9. Then, motor 3 is turned on to drive helical gear 4 to rotate, which in turn drives spur gear 5 to rotate. Spur gear 5 drives rotating shaft 6 to rotate. Spur gear 5 in other positions on rotating shaft 6 also rotates, causing other helical gears 4 meshing with spur gear 5 to rotate, which drives rotating shaft 7 to rotate. This causes the paddle 8 on rotating shaft 7 to rotate, stirring the liquid and bringing the liquid at the bottom to the top. A fixing plate 12 is fixed on the top of container 1, which restricts the position of rotating shaft 6, preventing it from rubbing against the bottom of container 1 and causing contamination. Finally, valve 21 is opened to allow the liquid to flow out from discharge pipe 20, achieving gentle stirring of the liquid and preventing it from sinking to the bottom and causing contamination.

[0040] When using the equipment, the temperature needs to be maintained within a certain range. A condenser tube 202 and a heat dissipation tube 201 are wound around the outer wall of the container 1. Coolant flows inside the condenser tube 202, and heat transfer oil flows inside the heat dissipation tube 201. The condenser tube 202 is connected to the refrigerator 204 through the connecting pipe 203, and the heat dissipation tube 201 is connected to the compressor 205 through the connecting pipe 203. Under normal circumstances, the refrigerator 204 and the compressor 205 keep the temperature fixed at a suitable temperature. If the temperature inside the equipment is too high, the refrigerator 204 lowers the temperature of the coolant in the condenser tube 202 to cool the equipment. If the temperature is too low, the compressor 205 is turned on to raise the temperature of the heat transfer oil in the heat dissipation tube 201 to heat the equipment, thus achieving the function of controlling the equipment temperature within a certain range.

[0041] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. An NK cell-modified stirrer, comprising a container (1), characterized in that: A fixed plate (12) is fixedly connected to the top of the container (1), a motor (3) is fixedly connected to the top of the fixed plate (12), a helical gear (4) is fixedly connected to the output end of the motor (3), a spur gear (5) is meshed with the outer wall of the helical gear (4), a rotating shaft (6) is fixedly connected to the inner wall of the spur gear (5), a rotating shaft (7) is meshed with the left and right sides of the rotating shaft (6), a paddle (8) is fixedly connected to the outer wall of the rotating shaft (7), a waterproof box (10) is rotatably connected to the outer wall of the rotating shaft (7), a rotating bearing (11) is fixedly connected to the inner wall of the waterproof box (10), a water inlet (9) is opened on the front side of the fixed plate (12), and a temperature control mechanism (2) is fixedly connected to the outer wall of the container (1). The temperature control mechanism (2) is used to control the temperature.

2. The NK cell-modified stirrer according to claim 1, characterized in that: The temperature control mechanism (2) includes a heat dissipation pipe (201), the outer wall of which is fixedly connected to the outer wall of the container (1), a condenser pipe (202) is fixedly connected to the outer wall of the container (1), an insulation shell (208) is slidably connected to the outer wall of the heat dissipation pipe (201), an equipment shell (206) is fixedly connected to the outer wall of the insulation shell (208), a refrigerator (204) is fixedly connected to the inner wall of the equipment shell (206), a compressor (205) is fixedly connected to the inner wall of the equipment shell (206), a connecting pipe (203) is connected to the output end of the compressor (205), and a booster pump (207) is fixedly connected to the outer wall of the heat dissipation pipe (201).

3. The NK cell-modified stirrer according to claim 1, characterized in that: The bottom of the container (1) is fixedly connected to a load-bearing plate (16), and the bottom of the load-bearing plate (16) is fixedly connected to a support column (17).

4. The NK cell-modified stirrer according to claim 2, characterized in that: The bottom of the refrigerator (204) is fixedly connected to a fixing column (18), and the bottom of the compressor (205) is fixedly connected to a fixing column (18).

5. The NK cell-modified stirrer according to claim 2, characterized in that: The top of the container (1) is fixedly connected to a top cover (13), and the inner wall of the container (1) is fixedly connected to a discharge pipe (20).

6. The NK cell-modified stirrer according to claim 2, characterized in that: The outer wall of the heat dissipation pipe (201) is fixedly connected with a heat insulation layer (15), and the outer wall of the condenser pipe (202) is fixedly connected with a heat insulation layer (15).

7. The NK cell-modified stirrer according to claim 5, characterized in that: A valve (21) is rotatably connected to the outer wall of the discharge pipe (20), and a water pump (19) is fixedly connected to the outer wall of the discharge pipe (20).

8. The NK cell-modified stirrer according to claim 5, characterized in that: The outer wall of the top cover (13) is rotatably connected to a flip cover (14), and a handle (22) is fixedly connected to the top of the flip cover (14).

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