Ultrafiltration centrifuge cup

Abstract

The utility model belongs to centrifugal experiment technical field provides an ultrafiltration centrifugal cup, include: lower cup body, lower cup body has the collection cavity for collecting filtrate, and the top of lower cup body is provided with the open mouth, flow channel ring, flow channel ring at least partial from open mouth stretches into lower cup body to install on lower cup body, and flow channel ring has the mounting hole along the axial penetration, cup body, upper cup body includes the body part and the installation part that are connected, the body part has the holding cavity for holding the sample to be filtered, and the top of body part is provided with the sample inlet, and the installation part is matched with the mounting hole, and the both sides of installation part are provided with the installation position for installing the filter membrane, and the installation part is provided with the sample groove that communicates with the holding cavity on, the utility model discloses to the processing of the biological protein solution of large solution volume, can be handled using an ultrafiltration centrifugal cup, need not use multiple ultrafiltration centrifugal tube, avoid the increase of the retention volume brought by multiple ultrafiltration centrifugal tube, improve the recovery rate.

Description

Technical Field

[0001] This utility model relates to the field of centrifugation experimental technology, and in particular to an ultrafiltration centrifuge cup. Background Technology

[0002] Centrifugation is one of the most commonly used methods for separating proteins, enzymes, nucleic acids, and cellular subcomponents. It is also a commonly used method for separation, purification, or clarification in biochemical laboratories. With the in-depth development of life science research, the demand for high-purity, rapid separation, and concentration of biological samples is increasing.

[0003] In related technologies, ultrafiltration centrifuge tubes are commonly used for experiments. The processing capacity of ultrafiltration centrifuge tubes for biological protein solutions is typically 20 ml. When processing large volumes of biological protein solutions of 20-100 ml, multiple ultrafiltration centrifuge tubes are usually required. However, using multiple ultrafiltration centrifuge tubes will lead to a significant increase in the retention volume, resulting in a decrease in the overall recovery rate of the biological protein solution. On the other hand, flat sheet membrane products are complicated to use, requiring the connection of tubing. Furthermore, membrane packs are suitable for processing volumes of 100 ml or more. When the solution volume is small, it will lead to a large amount of solution waste. In addition, most traditional ultrafiltration tubes place a membrane at the bottom of the device. During centrifugation, the solution is pressed vertically against the filter membrane, and the solution (such as protein) quickly accumulates on the membrane surface, forming a "concentration polarization" layer, which clogs the filter membrane and causes a sharp drop in filtration speed. Utility Model Content

[0004] This invention provides an ultrafiltration centrifuge cup to solve the technical problem of low recovery rate and efficiency of existing ultrafiltration centrifuge tubes when processing large volumes of biological protein solutions.

[0005] This utility model provides an ultrafiltration centrifuge cup, the ultrafiltration centrifuge cup comprising:

[0006] The lower cup body has a collection chamber for collecting the filtered material, and the top of the lower cup body is provided with an opening;

[0007] A flow channel ring, at least partially extending from the opening into the lower cup body for mounting on the lower cup body, the flow channel ring having an axially penetrating mounting hole;

[0008] The upper cup body includes a body portion and a mounting portion connected to each other. The body portion has a receiving cavity for accommodating the sample to be filtered. The top of the body portion is provided with a sample inlet. The mounting portion matches the mounting hole. The mounting portion has mounting positions for mounting a filter membrane on both sides. The mounting portion has a sample inlet groove communicating with the receiving cavity. The mounting portion extends into the mounting hole. The body portion contacts the flow channel ring.

[0009] A lid is detachably disposed at the opening for opening or closing the upper cup body.

[0010] In one embodiment of the present invention, a plurality of flow channel grooves are formed on the wall of the mounting hole which is arranged opposite to each other in the width direction. The plurality of flow channel grooves form a tooth-like structure on the wall of the mounting hole. The filter membrane is installed in the mounting position, and the mounting part extends into the mounting hole to cover the flow channel grooves with the filter membrane.

[0011] In one embodiment of the present invention, the flow channel ring is provided with a plurality of vent holes on both sides of the mounting hole, the vent holes are axially connected to the flow channel ring, and the flow channel ring is provided with a first vent groove on the end face near the upper cup body, the first vent groove being used to connect the plurality of vent holes.

[0012] In one embodiment of the present invention, the flow channel ring is further provided with a second exhaust groove on the end face near the upper cup body. The second exhaust groove is located on both sides of the mounting hole along its length. The second exhaust groove connects the mounting hole and the first exhaust groove and forms an exhaust port on the outer side wall of the flow channel ring.

[0013] In one embodiment of the present invention, the flow channel ring includes a large-diameter portion and a small-diameter portion connected together. The diameter of the large-diameter portion is the same as the outer diameter of the lower cup body, and the diameter of the small-diameter portion is the same as the inner diameter of the lower cup body. The small-diameter portion extends into the lower cup body from the opening until the large-diameter portion contacts the lower cup body.

[0014] In one embodiment of the present invention, a ventilation groove is provided on the outer wall of the small diameter portion along the axial direction, and the ventilation groove extends to the bottom end of the large diameter portion and forms a ventilation port on the outer wall of the large diameter portion.

[0015] In one embodiment of this utility model, the outer wall of the small-diameter portion is provided with a plurality of guide ribs or threads along the circumferential direction.

[0016] In one embodiment of this utility model, the mounting part is a ladder structure, and the angle between the inclined surface and the center surface of the ladder structure is 2-4°.

[0017] In one embodiment of the present invention, the included angle between the inner bottom wall and the outer bottom wall of the main body is 2-4°.

[0018] In one embodiment of this utility model, a vent hole is provided on the cover.

[0019] The beneficial effects of this utility model are as follows: The ultrafiltration centrifuge cup proposed in this utility model first opens the lid, and adds the sample to be filtered into the upper cup body through the sample inlet. At least part of the sample enters the mounting part from the main body. Then, the lid is closed to seal the upper cup body, and centrifugation is performed. Under centrifugation, the sample to be filtered passes through the filter membrane at the mounting position. The filtrate flows into the lower cup body for collection after passing through the flow channel ring. For the processing of large volumes of biological protein solutions, one ultrafiltration centrifuge cup can be used, eliminating the need for multiple ultrafiltration centrifuge tubes, avoiding the increase in retention volume caused by multiple ultrafiltration centrifuge tubes, improving the recovery rate and experimental efficiency. Attached Figure Description

[0020] The accompanying drawings, which are incorporated in and form part of this specification, illustrate embodiments consistent with this application and, together with the description, serve to explain the principles of this application. It is obvious that the drawings described below are merely some embodiments of this application, and those skilled in the art can obtain other drawings based on these drawings without any inventive effort.

[0021] In the attached diagram:

[0022] Figure 1 This is a schematic diagram of the structure of an ultrafiltration centrifuge cup provided in an embodiment of the present invention;

[0023] Figure 2 A first-view structural schematic diagram of the flow channel ring provided in an embodiment of the present invention;

[0024] Figure 3 A second-view structural schematic diagram of the flow channel ring provided in an embodiment of the present invention;

[0025] Figure 4 This is an assembly diagram of the flow channel ring and the upper cup body provided in one embodiment of the present invention;

[0026] Figure 5 This is a schematic diagram of the external structure of the upper cup body provided in an embodiment of the present utility model;

[0027] Figure 6 This is a schematic diagram of the internal structure of the lower cup body provided in an embodiment of the present invention.

[0028] The attached figures are labeled as follows:

[0029] 1-Lower cup body; 2-Flow channel ring; 3-Upper cup body; 4-Lid body; 5-Holding part; 6-Mounting hole; 7-Flow channel groove; 8-Exhaust hole; 9-First exhaust groove; 10-Second exhaust groove; 11-Large diameter part; 12-Small diameter part; 13-Vent groove; 14-Vent port; 15-Guide rib; 16-Receiving cavity; 17-Sample inlet groove; 18-Exhaust port; 19-Main body; 20-Mounting part; 21-Mounting position; 22-Connecting structure; 23-Inner bottom wall; 24-Outer bottom wall; 25-Inclined surface; 26-Center surface. Detailed Implementation

[0030] The following specific examples illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification. This utility model can also be implemented or applied through other different specific embodiments. Various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of this utility model. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other.

[0031] It should be noted that the illustrations provided in the following embodiments are only schematic representations of the basic concept of the present invention. The drawings only show the components related to the present invention and are not drawn according to the actual number, shape and size of the components. In actual implementation, the form, quantity and proportion of each component can be arbitrarily changed, and the layout of the components may also be more complex.

[0032] In the following description, numerous details are explored to provide a more thorough explanation of embodiments of the present invention. However, it will be apparent to those skilled in the art that embodiments of the present invention may be practiced without these specific details. In other embodiments, well-known structures and devices are shown in block diagram form rather than in detail to avoid obscuring embodiments of the present invention.

[0033] Please see Figures 1 to 6 This utility model exemplarily provides an ultrafiltration centrifuge cup, comprising:

[0034] The lower cup body 1 has a collection chamber for collecting the filtered material, and the top of the lower cup body 1 is provided with an opening;

[0035] The flow channel ring 2 extends at least partially from the opening into the lower cup body 1 for mounting on the lower cup body 1, and the flow channel ring 2 has a mounting hole 6 extending through it in the axial direction;

[0036] The upper cup body 3 includes a body part 19 and a mounting part 20 connected to each other. The body part 19 has a receiving cavity 16 for receiving the sample to be filtered. The top of the body part 19 is provided with a sample inlet. The mounting part 20 matches the mounting hole 6. The mounting part 20 has mounting positions 21 for mounting the filter membrane on both sides. The mounting part 20 has a sample inlet groove 17 that communicates with the receiving cavity 16. The mounting part 20 extends into the mounting hole 6. The body part 19 contacts the flow channel ring 2.

[0037] The lid 4 is detachably mounted at the opening and is used to open or close the upper cup 3.

[0038] The filter membrane can be installed at the mounting position in the following ways: adhesive bonding, hot melting, ultrasonic welding, interference fit of sealing ring, etc. The installation method of the filter membrane is not limited here.

[0039] It should be noted that current methods for concentrating large-volume biological protein solutions mainly employ multiple ultrafiltration centrifuge tubes or flat-plate ultrafiltration membrane packs. However, both methods have shortcomings. Since the maximum processing capacity of an ultrafiltration centrifuge tube is 20 ml, processing large volumes of biological protein solutions (20-100 ml) requires multiple ultrafiltration centrifuge tubes. Using multiple ultrafiltration centrifuge tubes not only increases costs but also leads to a significant increase in retention volume, resulting in a decrease in the overall protein solution recovery rate. Flat-plate membrane packs are complex to use, requiring tubing connections, and are suitable for processing volumes of 100 ml. When the solution volume is greater than 100ml, it will result in significant solution waste. Therefore, existing ultrafiltration equipment cannot meet the processing requirements of biological protein solutions with a volume of 20-100ml. In addition, traditional ultrafiltration centrifuge tubes place a membrane at the bottom of the device. During centrifugation, the solution is pressed vertically against the filter membrane, and the solution (such as protein) quickly accumulates on the membrane surface, forming a "concentration polarization" layer, which clogs the filter membrane and causes a sharp drop in filtration speed. The ultrafiltration centrifuge cup of this application can process a maximum of 100ml of biological protein, which can meet the processing requirements of biological protein solutions with a volume of 20-100ml.

[0040] In the ultrafiltration centrifuge cup provided in this embodiment, the cover 4 is first opened, and the sample to be filtered is added into the upper cup 3 through the sample inlet. At least part of the filtered sample enters the mounting part 20 from the main body 19. Then, the cover 4 is closed to seal the upper cup 3, and centrifugation is performed. Under centrifugation, the sample to be filtered passes through the filter membrane at the mounting position 21. The filtered material flows into the lower cup 1 for collection after passing through the flow channel ring 2. For the processing of large volumes of biological protein solutions, one ultrafiltration centrifuge cup can be used, eliminating the need for multiple ultrafiltration centrifuge tubes, avoiding the increase in retention volume caused by multiple ultrafiltration centrifuge tubes, improving the recovery rate and experimental efficiency.

[0041] In this embodiment, multiple flow channel grooves 7 are formed on the wall of the mounting hole 6, which is arranged opposite to each other in the width direction. The multiple flow channel grooves 7 form a tooth-like structure on the wall of the mounting hole 6. The filter membrane is installed on the mounting position 21, and the mounting part 20 extends into the mounting hole 6 to cover the filter membrane on the flow channel grooves 7. Specifically, the mounting part 20 and the wall of the mounting hole 6 press the two sides of the filter membrane together, so that the filter membrane fits tightly with the tooth-like structure, ensuring the stability of the filter membrane and preventing the filter membrane from breaking during high-speed centrifugation. In order to expand the usable area of ​​the filter membrane, the distance between the flow channel grooves 7 can be appropriately increased, or a certain distance can be left un-set near the circumferential edge of the flow channel grooves 7 on the left and right sides.

[0042] In some embodiments, the flow channel ring 2 has multiple exhaust holes 8 on both sides of the mounting hole 6. The exhaust holes 8 extend through the flow channel ring 2 axially. The flow channel ring 2 has a first exhaust groove 9 on the end face near the upper cup body 3. The first exhaust groove 9 is used to connect the multiple exhaust holes 8. Specifically, when the upper cup body 3 and the flow channel ring 2 are assembled, the end face of the flow channel ring 2 near the upper cup body 3 contacts the upper cup body 3. The upper part of the first exhaust groove 9 is closed, and the first exhaust groove 9 forms an air flow channel. During centrifugation, air flows into the first exhaust groove 9 from the multiple exhaust holes 8.

[0043] In the above embodiment, the flow channel ring 2 is also provided with a second exhaust groove 10 on the end face near the upper cup body 3. The second exhaust groove 10 is located on both sides of the mounting hole 6 along its length. The second exhaust groove 10 connects the mounting hole 6 and the first exhaust groove 9, and forms an exhaust port 18 on the outer side wall of the flow channel ring 2. Specifically, during the centrifugation process, air may also enter from the gap between the mounting hole 6 and the mounting part 20. The second exhaust groove 10 connects the mounting hole 6 and the first exhaust groove 9 to the outside. Air entering the first exhaust groove 9 from the exhaust port 8 or entering the second exhaust groove 10 from the mounting hole 6 will be discharged from the exhaust port 18, which avoids excessive air entering and affecting the centrifugal filtration effect and ensures the air pressure balance inside the entire structure.

[0044] In detail, the flow channel ring 2 includes a large-diameter portion 11 and a small-diameter portion 12 connected to each other. The diameter of the large-diameter portion 11 is the same as the outer diameter of the lower cup body 1, and the diameter of the small-diameter portion 12 is the same as the inner diameter of the lower cup body 1. The small-diameter portion 12 extends into the lower cup body 1 from the opening until the large-diameter portion 11 contacts the lower cup body 1. The large-diameter portion 11 and the small-diameter portion 12 are coaxially arranged. A limiting structure is formed at the connection between the large-diameter portion 11 and the small-diameter portion 12 to limit the distance that the flow channel ring 2 extends into the lower cup body 1. When the small-diameter portion 12 of the flow channel ring 2 extends into the lower cup body 1 from the opening and the large-diameter portion 11 contacts the lower cup body 1, the limiting structure will limit the flow channel ring 2 from continuing to extend into the lower cup body 1, thereby realizing the installation of the flow channel ring 2 in the lower cup body 1.

[0045] Furthermore, the outer wall of the small diameter portion 12 is provided with a ventilation groove 13 along the axial direction. The ventilation groove 13 extends to the bottom end of the large diameter portion 11 and forms a ventilation port 14 on the outer wall of the large diameter portion 11. When the flow channel ring 2 is installed in the lower cup body 1, the lower cup body 1 and the large diameter portion 11 are fitted together, and the flow channel ring 2 and the lower cup body 1 are spliced ​​into a whole. At this time, a ventilation port 14 is formed on the outer wall. The ventilation groove 13 allows the outside world to communicate with the inside of the lower cup body 1. During the centrifugal treatment process, the filtered material flows into the lower cup body 1, and the air in the lower cup body 1 is discharged through the ventilation groove 13 and the ventilation port 14.

[0046] In the above embodiment, the outer side wall of the small diameter portion 12 is provided with a plurality of guide ribs 15 or threads in the circumferential direction. Specifically, the guide ribs 15 are distributed along the length direction of the small diameter portion 12, and the thickness of the guide ribs 15 gradually increases in the direction away from the lower cup body 1. The thickness of the guide ribs 15 is very small, and can provide guidance when the small diameter portion 12 extends into the lower cup body 1. The plurality of guide ribs 15 are evenly distributed in the circumferential direction of the outer side wall of the small diameter portion 12, which can reduce the assembly gap between the outer side wall of the small diameter portion 12 and the inner side wall of the lower cup body 1, so that the assembly between the flow channel ring 2 and the lower cup body 1 is tighter. Alternatively, the flow channel ring 2 and the lower cup body 1 are connected by threads, that is, the outer side wall of the small diameter portion 12 is provided with threads, and the inner side wall of the lower cup body 1 is provided with threaded grooves.

[0047] In some embodiments, the mounting part 20 is a trapezoidal structure, and the angle between the inclined surface 25 and the center surface 26 of the trapezoidal structure is 2-4°. Specifically, the mounting part 20 is located in the middle of the end face of the body part 19, and the mounting hole 6 is located in the middle of the flow channel ring 2. The outer diameter of the large diameter part 11 of the flow channel ring 2 is the same as the outer diameter of the body part 19 of the upper cup body 3. When the mounting part 20 of the upper cup body 3 extends into the mounting hole 6, the large diameter part 11 and the body part 19 are spliced ​​together as a whole, and the shape of the mounting hole 6 matches that of the mounting part 20. With this design of the inclined surface 25, the filter membrane is installed on the mounting position 21 of the mounting part 20, and the inclination angle of the filter membrane is also 2-4°. This can prevent the solution from being pressed vertically onto the filter membrane, causing the solution (such as protein) to accumulate on the membrane surface and block the filter membrane, thereby improving the filtration speed. Preferably, in this embodiment, the included angle of the inclined surface 25 of the mounting part 20 and the inclination angle of the filter membrane are both 3.5°. This is not limited here.

[0048] In this embodiment, the angle between the inner bottom wall 23 and the outer bottom wall 24 of the main body 19 is 2-4°. This angle design makes the bottom of the receiving cavity 16 tilted. When the sample to be filtered is added to the upper cup 3, the sample can more easily flow from the receiving cavity 16 into the sample inlet 17. When the filter membrane is installed at the mounting position 21, the filter membrane closes the mounting position 21, allowing the sample to be filtered to remain in the sample inlet 17. During the centrifugation process, the filtrate is filtered out by the filter membrane and flows into the lower cup 1 through the flow channel 7. The sample to be filtered in the receiving cavity 16 will continuously enter the sample inlet 17 for replenishment. The tilted inner bottom wall 23 can reduce the retention volume in the receiving cavity 16. Compared with the traditional filtration method of multiple ultrafiltration centrifuge tubes, it can effectively reduce the retention volume and reduce costs.

[0049] In some embodiments, a vent is provided on the cover 4. Specifically, the vent is located in the middle of the cover 4, and air inside the upper cup 3 can be discharged through the vent.

[0050] It is understood that the lid 5 and the upper cup 3 can be connected by means of interference fit, threaded connection or snap-fit ​​connection. The connection structure 22 can be adjusted according to the connection method. In this embodiment, the upper cup 3 is also provided with a connection structure 22 along the circumferential direction on the outer side wall of the body part 19 for installing the lid 5 at the opening. The connection structure 22 is a threaded structure, and the upper cup 3 and the lid 5 are connected by a thread. The connection method between the two is not limited here.

[0051] In this embodiment, a recessed gripping part 5 is provided at the connection between the upper cup body 3 and the flow channel ring 2 to facilitate the removal of the ultrafiltration centrifuge cup.

[0052] In summary, in the ultrafiltration centrifuge cup provided in this embodiment, the cover 4 is first opened, and the sample to be filtered is added into the upper cup 3 through the sample inlet. At least part of the filtered sample enters the mounting part 20 from the main body 19. Then, the cover 4 is closed to seal the upper cup 3, and centrifugation is performed. Under centrifugation, the sample to be filtered passes through the filter membrane at the mounting position 21. The filtrate flows into the lower cup 1 for collection after passing through the flow channel ring 2. For the processing of large volumes of biological protein solutions, one ultrafiltration centrifuge cup can be used, eliminating the need for multiple ultrafiltration centrifuge tubes, avoiding the increase in retention volume caused by multiple ultrafiltration centrifuge tubes, improving the recovery rate and experimental efficiency.

[0053] The above embodiments are merely illustrative of the principles and effects of this utility model and are not intended to limit the scope of this utility model. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of this utility model. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in this utility model should still be covered by the claims of this utility model.

Claims

1. An ultrafiltration centrifuge cup, characterized in that, include: The lower cup body has a collection chamber for collecting the filtered material, and the top of the lower cup body is provided with an opening; A flow channel ring, at least partially extending from the opening into the lower cup body for mounting on the lower cup body, the flow channel ring having an axially penetrating mounting hole; The upper cup body includes a body portion and a mounting portion connected to each other. The body portion has a receiving cavity for accommodating the sample to be filtered. The top of the body portion is provided with a sample inlet. The mounting portion matches the mounting hole. The mounting portion has mounting positions for mounting a filter membrane on both sides. The mounting portion has a sample inlet groove communicating with the receiving cavity. The mounting portion extends into the mounting hole. The body portion contacts the flow channel ring. A lid is detachably disposed at the opening for opening or closing the upper cup body.

2. The ultrafiltration centrifuge cup according to claim 1, characterized in that, The mounting hole has multiple flow channel grooves on its wall, which are arranged opposite each other along the width direction. The multiple flow channel grooves form a tooth-like structure on the wall of the mounting hole. The filter membrane is installed in the mounting position, and the mounting part extends into the mounting hole to cover the flow channel grooves with the filter membrane.

3. The ultrafiltration centrifuge cup according to claim 1, characterized in that, The flow channel ring has multiple vent holes on both sides of the mounting hole. The vent holes extend through the flow channel ring axially. The flow channel ring has a first vent groove on the end face near the upper cup body. The first vent groove is used to connect the multiple vent holes.

4. The ultrafiltration centrifuge cup according to claim 3, characterized in that, The flow channel ring also has a second exhaust groove on the end face near the upper cup body. The second exhaust groove is located on both sides of the mounting hole along its length. The second exhaust groove connects the mounting hole and the first exhaust groove, and forms an exhaust port on the outer wall of the flow channel ring.

5. The ultrafiltration centrifuge cup according to claim 1, characterized in that, The flow channel ring includes a large-diameter portion and a small-diameter portion connected together. The diameter of the large-diameter portion is the same as the outer diameter of the lower cup body, and the diameter of the small-diameter portion is the same as the inner diameter of the lower cup body. The small-diameter portion extends into the lower cup body from the opening until the large-diameter portion contacts the lower cup body.

6. The ultrafiltration centrifuge cup according to claim 5, characterized in that, The outer wall of the small diameter section is provided with a ventilation groove along the axial direction, and the ventilation groove extends to the bottom end of the large diameter section and forms a ventilation port on the outer wall of the large diameter section.

7. The ultrafiltration centrifuge cup according to claim 5, characterized in that, The outer wall of the small diameter section is provided with multiple guide ribs or threads along the circumference.

8. The ultrafiltration centrifuge cup according to claim 1, characterized in that, The mounting part is a ladder structure, and the angle between the inclined surface and the center surface of the ladder structure is 2-4°.

9. The ultrafiltration centrifuge cup according to claim 1, characterized in that, The included angle between the inner bottom wall and the outer bottom wall of the main body is 2-4°.

10. The ultrafiltration centrifuge cup according to claim 1, characterized in that, The cover has ventilation holes.

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