A device for concentrating and purifying a biological enzyme

Abstract

The utility model discloses a kind of biological enzyme concentration purification devices, including bucket, the bottom of barrel is fixedly connected with conical material guide shell, the bottom of conical material guide shell is fixedly connected with discharge pipe, the top of barrel is fixedly connected with feed pipe, the barrel wall of barrel is equipped with cavity, heating wire is equipped in cavity, the top outer wall of barrel is fixedly connected with motor, the one end of motor output shaft passes through barrel and is connected with rotating shaft by coupling, the outside of rotating shaft is fixedly connected with two symmetrical distribution's L type scraper frame, the bottom of L type scraper frame is fixedly connected with the scraping blade that is in conformity with the inner wall of conical material guide shell.This utility model not only can make biological enzyme solution to heat evenly, avoid local overheating to cause enzyme denaturation inactivation, save concentration time simultaneously, improve efficiency, and can assist the biological enzyme of concentration completion to discharge, avoid plugging when discharging.

Description

Technical Field

[0001] This utility model relates to the field of bioenzyme purification technology, and in particular to a bioenzyme concentration and purification device. Background Technology

[0002] Bioenzymes, often simply called enzymes, are a class of proteins with biocatalytic activity. Currently, in industries such as biopharmaceuticals and food processing, bioenzymes are used as important catalytic tools. In order to improve product quality during their production process, bioenzymes need to be concentrated and purified.

[0003] A search revealed that CN218855092U discloses a cleaning mechanism and a bio-enzyme concentration device, including a cover plate and a curved scraper. A motor is located at the upper center of the cover plate, and a connecting shaft is located at the lower center of the cover plate. However, the above device uses bottom heating to concentrate the bio-enzyme solution, and the bio-enzyme solution cannot be stirred inside the chamber, resulting in local overheating of the bio-enzyme solution, causing enzyme denaturation and inactivation, and affecting the bio-enzyme concentration and purification effect. In order to better address the above problems, promote the development of industry technology, and improve core competitiveness, this application proposes a new composition structure that is different from the prior art. Utility Model Content

[0004] The purpose of this invention is to address the shortcomings of existing technologies by proposing a biological enzyme concentration and purification device.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A bio-enzyme concentration and purification device includes a barrel body. A conical guide shell is fixedly connected to the bottom of the barrel body, and a discharge pipe is fixedly connected to the bottom of the conical guide shell. A feed pipe is fixedly connected to the top of the barrel body. A cavity is formed inside the barrel body, and a heating wire is installed in the cavity. A motor is fixedly connected to the top outer wall of the barrel body. One end of the motor output shaft passes through the barrel body and is connected to a rotating shaft via a coupling. Two symmetrically distributed L-shaped scrapers are fixedly connected to the outside of the rotating shaft. A scraper that fits against the inner wall of the conical guide shell is fixedly connected to the bottom of the L-shaped scraper. A connecting plate is fixedly connected between the L-shaped scraper and the rotating shaft. A connecting shaft is rotatably connected between the connecting plate and the L-shaped scraper via a waterproof bearing. Multiple inclined stirring plates are fixedly connected to the outside of the connecting shaft. Multiple stirring racks are fixedly connected to the outside of the connecting shaft. Multiple stirring plates are fixedly connected to the bottom of the connecting plate. A condensation component for condensing steam into water is provided on the outside of the barrel body.

[0007] As a further embodiment of this utility model, the condensation assembly includes an exhaust pipe, which is fixedly connected to the top of the barrel. One end of the exhaust pipe is fixedly connected to a condenser pipe, and one end of the condenser pipe is fixedly connected to a T-connector. A support plate is fixedly connected to one side of the outer wall of the barrel, and a collection box is fixedly connected to the upper surface of the support plate, with the T-connector located directly above the collection box.

[0008] As a further embodiment of this utility model, the condenser tube is in a downward-sloping state at one end connected to the tee tube, and a water outlet pipe is fixedly connected to one side of the collection box at the bottom position.

[0009] As a further improvement of this utility model, valves are provided on the outer sides of both the water outlet pipe and the material outlet pipe.

[0010] As a further embodiment of this utility model, one end of the rotating shaft extends into the discharge pipe, and an auxiliary discharge spiral blade is fixedly connected to the outside of the rotating shaft.

[0011] As a further embodiment of this utility model, a fixing frame is fixedly connected to the outside of the barrel, and a cap is snapped onto the top of the feed pipe.

[0012] As a further embodiment of this invention, the inclination angle of the first stirring plate and the second stirring plate is 5-15°.

[0013] The beneficial effects of this utility model are as follows:

[0014] 1. In this utility model, the biological enzyme solution is heated by heating from all sides, and the biological enzyme solution is stirred by stirring plate one, stirring rack and stirring plate two, so that the biological enzyme solution can be heated evenly, avoiding local overheating that would cause enzyme denaturation and inactivation, while saving concentration time and improving efficiency.

[0015] 2. In this utility model, by setting up a condensation component, when heating and concentrating the biological enzyme solution, the heated water vapor will be condensed into water through the condensation component, and the condensate will be collected and reused, thereby improving the utilization rate of resources.

[0016] 3. In this invention, by setting the spiral plate, after the biological enzyme solution is heated and concentrated, the protein in the biological enzyme will denature and aggregate, increasing the viscosity of the solution. At this time, the spiral plate can assist the concentrated biological enzyme in discharging, avoiding blockage during discharging. Attached Figure Description

[0017] Figure 1 This is a three-dimensional structural diagram of a biological enzyme concentration and purification device proposed in this utility model.

[0018] Figure 2This is a cross-sectional structural diagram of a biological enzyme concentration and purification device proposed in this utility model;

[0019] Figure 3 This is a partially enlarged structural diagram of a biological enzyme concentration and purification device proposed in this utility model.

[0020] In the diagram: 1. Barrel body; 2. Feed pipe; 3. Cover; 4. Motor; 5. Gas outlet pipe; 6. Condenser pipe; 7. T-pipe; 8. Collection box; 9. Water outlet pipe; 10. Support plate; 11. Fixing frame; 12. Conical guide shell; 13. Discharge pipe; 14. Cavity; 15. Heating wire; 16. Rotating shaft; 18. L-shaped scraper; 19. Connecting shaft; 20. Stirring plate one; 21. Stirring frame; 22. Connecting plate; 23. Stirring plate two; 24. Spiral blade; 25. Scraper. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. The described embodiments are only some embodiments of the present utility model, not all embodiments. Other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are all within the protection scope of the present utility model.

[0022] Reference Figures 1-3 A bio-enzyme concentration and purification device includes a barrel 1, a conical guide shell 12 welded to the bottom of the barrel 1, a discharge pipe 13 welded to the bottom of the conical guide shell 12, a feed pipe 2 welded to the top of the barrel 1, and a cavity 14 opened in the barrel wall of the barrel 1. A heating wire 15 is installed in the cavity 14. When concentration is required, the bio-enzyme solution to be concentrated is added into the barrel 1 through the feed pipe 2, and then the heating wire 15 is activated to heat the bio-enzyme solution in the barrel 1, causing the water in the bio-enzyme solution to evaporate, thereby achieving the concentration of the bio-enzyme solution.

[0023] In this invention, a motor 4 is bolted to the top outer wall of the barrel 1. One end of the output shaft of the motor 4 passes through the barrel 1 and is connected to a rotating shaft 16 via a coupling. Two symmetrically distributed L-shaped scrapers 18 are welded to the outside of the rotating shaft 16. A scraper 25 that fits against the inner wall of the conical guide shell 12 is welded to the bottom of the L-shaped scraper 18. A connecting plate 22 is welded between the L-shaped scraper 18 and the rotating shaft 16. A connecting shaft 19 is rotatably connected between the connecting plate 22 and the L-shaped scraper 18 via a waterproof bearing. Multiple inclined stirring plates 20 are welded to the outside of the connecting shaft 19. Multiple stirring racks 21 are bolted to the outside of the connecting shaft 19. During the concentration process, motor 4 is started, which drives the rotating shaft 16 to rotate at a reduced speed. The rotating shaft 16 drives the L-shaped scraper 18 and the connecting plate 22 to rotate. The connecting plate 22 drives the connecting shaft 19 to rotate. The connecting shaft 19 drives the stirring plate 20 and the stirring rack 21 to rotate, so that the stirring plate 20 and the stirring rack 21 agitate the biological enzyme solution. During the rotation of the stirring plate 20 and the stirring rack 21, under the action of centrifugal force and liquid flow, the stirring plate 20 and the stirring rack 21 will rotate on their own through the connecting shaft 19, thereby disturbing the biological enzyme solution and making the biological enzyme solution evenly exchange up and down and left and right.

[0024] Multiple stirring plates 23 are welded to the bottom of the connecting plate 22. During the stirring process, the connecting plate 22 will drive the stirring plates 23 to rotate, so that the stirring plates 23 can stir the biological enzyme solution in the conical guide shell 12. In this way, the biological enzyme solution is heated from all sides during the stirring process, so that the biological enzyme solution is heated evenly, avoiding local overheating that could lead to enzyme denaturation and inactivation, and saving concentration time.

[0025] During the agitation of the bio-enzyme solution, the L-shaped scraper 18 and scraper 25 scrape and clean the inner wall of the tank 1 and the inner wall of the conical guide shell 12, thereby preventing the bio-enzyme solution from adhering to the inner wall. When the device needs to be cleaned, cleaning water is added to the tank 1 and then the inner wall is scraped by the L-shaped scraper 18 and scraper 25, which facilitates the cleaning of the device. Moreover, the agitation and cleaning are achieved through a single power source, which saves costs and reduces energy consumption.

[0026] In this invention, a condensation assembly for condensing steam into water is provided on the outer side of the barrel body 1. The condensation assembly includes an outlet pipe 5, which is welded to the top of the barrel body 1. One end of the outlet pipe 5 is fixed to a condenser pipe 6 by bolts, and the other end of the condenser pipe 6 is fixed to a three-way pipe 7 by bolts. A support plate 10 is welded to one side of the outer wall of the barrel body 1. A collection box 8 is fixed to the upper surface of the support plate 10 by bolts, and the three-way pipe 7 is located directly above the collection box 8. The condenser pipe 6 is in a downward inclined state at one end connected to the three-way pipe 7. A water outlet pipe 9 is welded to one side of the collection box 8 at the bottom. Valves are provided on the outer sides of both the water outlet pipe 9 and the discharge pipe 13. Water vapor generated during the heating and concentration of the biological enzyme solution will enter the condenser pipe 6 through the outlet pipe 5 and be condensed by the condenser pipe 6. The condensed water will drip into the collection box 8 through the three-way pipe 7, while the gas will be discharged through the upper end of the three-way pipe 7, thereby realizing the collection and reuse of the condensed water and improving the utilization rate of resources.

[0027] In particular, one end of the rotating shaft 16 extends into the discharge pipe 13, and a spiral blade 24 for auxiliary discharge is welded to the outside of the rotating shaft 16. After concentration, the protein in the biological enzyme denatures and aggregates, increasing the viscosity of the solution. The concentrated biological enzyme is discharged through the discharge pipe 13. At the same time, the rotating shaft 16 drives the spiral blade 24 to rotate. The spiral blade 24 assists in the discharge of the material in the discharge pipe 13, preventing blockage. A fixing bracket 11 is fixed to the outside of the barrel 1 by bolts. A cap 3 is snapped onto the top of the feed pipe 2. The tilt angle of the first stirring plate 20 and the second stirring plate 23 is 5-15°. Due to the 5-15° tilt of the first stirring plate 20 and the second stirring plate 23, the symmetrical flow can be broken during the stirring process, generating vortices or asymmetrical shear forces, promoting the exchange of materials up and down and left and right, reducing dead corners, and improving the stirring effect.

[0028] Working principle: When concentration is required, the bio-enzyme solution to be concentrated is added into the tank 1 through the feed pipe 2. Then, the heating wire 15 is activated to heat the bio-enzyme solution in the tank 1, causing the water in the bio-enzyme solution to evaporate, thereby concentrating the bio-enzyme solution. At the same time, the motor 4 is activated, driving the rotating shaft 16 to rotate at a reduced speed. The rotating shaft 16 drives the L-shaped scraper 18 and the connecting plate 22 to rotate. The connecting plate 22 drives the connecting shaft 19 to rotate. The connecting shaft 19 drives the stirring plate 20 and the stirring frame 21 to rotate, causing the stirring plate 20 and the stirring frame 21 to agitate the bio-enzyme solution. During the rotation of the stirring plate 20 and the stirring frame 21, under the action of centrifugal force and liquid flow, the stirring plate 20 and the stirring frame 21 will agitate the solution. The frame 21 rotates via the connecting shaft 19, which turbulently flows the bio-enzyme solution, allowing for uniform exchange of the solution in all directions. Simultaneously, the connecting plate 22 drives the second stirring plate 23 to rotate, agitating the bio-enzyme solution within the conical feed shell 12. This agitation heats the bio-enzyme solution from all sides, ensuring uniform heating and preventing localized overheating that could lead to enzyme denaturation and inactivation. It also saves concentration time and improves efficiency. Furthermore, the 5-15° inclination of the first stirring plate 20 and the second stirring plate 23 breaks symmetrical flow during agitation, generating eddies or asymmetrical shear forces, promoting material exchange in all directions, reducing dead zones, and enhancing the agitation effect.

[0029] During the agitation of the bio-enzyme solution, the L-shaped scraper 18 and scraper 25 scrape and clean the inner wall of the barrel 1 and the inner wall of the conical guide shell 12, thereby preventing the bio-enzyme solution from adhering to the inner wall. When the device needs to be cleaned, cleaning water is added to the barrel 1 and then the inner wall is scraped by the L-shaped scraper 18 and scraper 25, which facilitates the cleaning of the device.

[0030] During the heating and concentration of the bio-enzyme solution, the water vapor generated enters the condenser tube 6 through the vent pipe 5. The condenser tube 6 condenses the water vapor, and the condensed water drips into the collection box 8 through the three-way pipe 7. The gas is discharged through the upper end of the three-way pipe 7, thus realizing the collection and reuse of the condensed water and improving the utilization rate of resources.

[0031] Once concentration is complete, the proteins in the bio-enzyme denature and aggregate, increasing the viscosity of the solution. The concentrated bio-enzyme is discharged through the discharge pipe 13. At the same time, the rotating shaft 16 drives the spiral blade 24 to rotate, and the spiral blade 24 assists in discharging the material in the discharge pipe 13 to prevent blockage.

[0032] Furthermore, the terms "installation," "setup," "connection," and "socketing" should be interpreted broadly. For example, they can refer to fixed connections, detachable connections, or integral constructions; they can refer to mechanical or electrical connections; they can refer to direct connections or indirect connections via an intermediate medium, or internal connections between two devices, components, or parts. Those skilled in the art can understand the specific meaning of these terms in this application based on the specific circumstances.

Claims

1. A biological enzyme concentration and purification device, comprising a barrel (1), characterized in that, A conical guide shell (12) is fixedly connected to the bottom of the barrel (1), and a discharge pipe (13) is fixedly connected to the bottom of the conical guide shell (12). A feed pipe (2) is fixedly connected to the top of the barrel (1). A cavity (14) is opened inside the barrel wall of the barrel (1), and a heating wire (15) is provided inside the cavity (14). A motor (4) is fixedly connected to the top outer wall of the barrel (1). One end of the output shaft of the motor (4) passes through the barrel (1) and is connected to a rotating shaft (16) through a coupling. Two L-shaped scrapers (18) are fixedly connected to the outside of the rotating shaft (16). The bottom of the L-shaped scraper (18) is fixedly connected to a scraper (25). A connecting plate (22) is fixedly connected between the L-shaped scraper (18) and the rotating shaft (16). A connecting shaft (19) is rotatably connected between the connecting plate (22) and the L-shaped scraper (18). Multiple inclined stirring plates (20) are fixedly connected to the outside of the connecting shaft (19). Multiple stirring racks (21) are fixedly connected to the outside of the connecting shaft (19). Multiple stirring plates (23) are fixedly connected to the bottom of the connecting plate (22). A condensation assembly is provided on the outside of the barrel (1).

2. The bioenzyme concentration and purification device according to claim 1, characterized in that, The condensation assembly includes an exhaust pipe (5), which is fixedly connected to the top of the barrel (1). One end of the exhaust pipe (5) is fixedly connected to a condenser pipe (6), and one end of the condenser pipe (6) is fixedly connected to a three-way pipe (7). A support plate (10) is fixedly connected to one side of the outer wall of the barrel (1), and a collection box (8) is fixedly connected to the upper surface of the support plate (10). The three-way pipe (7) is located directly above the collection box (8).

3. The bioenzyme concentration and purification device according to claim 2, characterized in that, The condenser tube (6) is in a downward tilted state at one end connected to the three-way pipe (7), and the water outlet pipe (9) is fixedly connected to one side of the collection box (8) at the bottom position.

4. The bioenzyme concentration and purification device according to claim 3, characterized in that, Valves are provided on the outside of both the water outlet pipe (9) and the material outlet pipe (13).

5. The bioenzyme concentration and purification device according to claim 1, characterized in that, One end of the rotating shaft (16) extends into the discharge pipe (13), and an auxiliary discharge spiral blade (24) is fixedly connected to the outside of the rotating shaft (16).

6. The bioenzyme concentration and purification apparatus according to claim 1, characterized in that, A fixing frame (11) is fixedly connected to the outside of the barrel (1), and a cap (3) is snapped onto the top of the feed pipe (2).

7. The bioenzyme concentration and purification device according to claim 1, characterized in that, The tilt angle of the first stirring plate (20) and the second stirring plate (23) is 5-15°.

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