General-purpose preparative chromatography column loading station system

Abstract

The utility model relates to a kind of general preparation chromatography column loading station systems, comprising: hydraulic station assembly, hydraulic cylinder assembly and preparation liquid chromatographic column;Multiple different specifications of the hydraulic cylinder assembly can be detachably installed on the hydraulic station assembly;The hydraulic station assembly and each hydraulic cylinder assembly are communicated by hydraulic oil pipeline, and different hydraulic oil oil pressure is provided for each hydraulic cylinder assembly;The preparation liquid chromatographic column includes chromatographic column cylinder and piston presser bar assembly;Multiple chromatographic column cylinders are supported on the hydraulic station assembly with interval, and multiple piston presser bar assemblies are supported on each chromatographic column cylinder, and top end is communicated with the cylinder center axis of each hydraulic cylinder assembly.According to the scheme of the utility model, the utility model sets different specifications of hydraulic cylinder assembly, loads different diameter specifications of preparation liquid chromatographic column, and guarantees the best loading pressure value and loading speed and other loading parameters.

Description

Technical Field

[0001] This utility model relates to the field of liquid chromatography technology, and in particular to a general-purpose preparative chromatography column packing station system. Background Technology

[0002] Compared to conventional chromatography, preparative chromatography can effectively separate and purify components in complex mixtures, achieving highly efficient separation. Therefore, preparative chromatography is widely used in industrial production and research institutions. A typical preparative chromatography system consists of a constant flow pump, a UV detector, and a chromatographic column. The most important component is the preparative column, which is also the key factor affecting the final preparation effect.

[0003] In routine laboratory settings, dynamic axial compression columns are rarely used due to cost considerations. Instead, various spring-loaded or static preparative columns of different sizes are typically used, depending on process requirements. Common diameters include 30mm, 50mm, 80mm, and 100mm. Currently available column packers require different chuck sizes to load preparative columns of varying diameters. While a single hydraulic cylinder is used, the loading pressure and speed requirements for each column size are inconsistent, making it impossible to load different column sizes with optimal parameters and compromising the packing effectiveness.

[0004] Existing column packers generally use a single hydraulic cylinder. Since the column packing parameters required for preparative chromatographic columns of different diameters vary greatly, it is difficult to stabilize the column packing parameters at the same error level for the same hydraulic cylinder. As a result, the column packing effect is unstable, meaning that the performance after column packing cannot be guaranteed. If different column packing machines are used for different diameter columns, the column packing effect can be guaranteed, but the purchase and use costs are high. The diameter of the central shaft of the hydraulic cylinder in each preparative chromatography column packer is fixed. In addition to ensuring safe and deformation-free transmission of large thrust during the packing process, the central shaft also partially enters the interior of the preparative chromatography column. If the same hydraulic cylinder is used, it is difficult to always match the central shaft of the hydraulic cylinder with column cylinders of different diameters. An extension rod is used to change the diameter of the central shaft of the cylinder, which is very inconvenient for customers to use. This not only affects the final column packing effect, but also poses a safety hazard. Utility Model Content

[0005] The purpose of this invention is to solve at least one technical problem in the background art and to provide a general-purpose preparative chromatography column packing station system.

[0006] To achieve the above objectives, this utility model provides a general-purpose preparative chromatography column packing station system, comprising: a hydraulic station assembly, a hydraulic cylinder assembly, and a preparative liquid chromatography column; Various hydraulic cylinder assemblies of different specifications can be detachably installed on the hydraulic station assembly; The hydraulic station assembly is connected to each of the hydraulic cylinder assemblies through hydraulic oil pipelines, providing different hydraulic oil pressures to each of the hydraulic cylinder assemblies. The prepared liquid chromatography column includes a column cylinder and a piston rod assembly; Multiple chromatographic column cylinders are supported at intervals on the hydraulic station assembly, and multiple piston rod assemblies are correspondingly supported on each of the chromatographic column cylinders, with their top ends connected to the central axis of each of the hydraulic cylinder assemblies.

[0007] According to one aspect of the present invention, the hydraulic station assembly includes: a support frame, a first support plate, and a second support plate; The first support plate is supported on the top of the support frame, and the first support plate is provided with a first mounting groove and a second mounting groove. The hydraulic cylinder assemblies of different specifications are detachably supported on the first mounting slot and the second mounting slot, respectively; The second support plate is supported at the bottom of the support frame and is arranged at a distance from the first support plate; The column barrels of the two preparative liquid chromatography columns are supported at intervals on the second support plate, and the piston rod assemblies of the two preparative liquid chromatography columns are correspondingly supported on each of the column barrels, with their top ends connected to the cylinder central axis of each of the hydraulic cylinder assemblies.

[0008] According to one aspect of the present invention, the hydraulic cylinder assembly includes: a bracket and a hydraulic cylinder; The support structure includes: a third support plate, a column, and a fourth support plate; The third support plate is provided with a U-shaped groove corresponding to the first mounting groove or the second mounting groove; Multiple columns are evenly spaced and supported on the third support plate; The fourth support plate is supported on top of the multiple columns; The hydraulic cylinder is supported on the fourth support plate, and its cylinder shaft extends through a through hole in the fourth support plate toward the third support plate. One end of the piston rod assembly for preparing the liquid chromatography column passes through the first mounting groove or the second mounting groove and the U-shaped groove and connects to the column cylinder; The other end of the piston rod assembly is connected to the central shaft of the hydraulic cylinder.

[0009] According to one aspect of the present invention, the hydraulic station assembly further includes: two pressure gauges, a regulating valve, a directional valve, and two switching valves; The pressure gauge is used to monitor and display the pressure value of the hydraulic oil output by the pneumatic pump that provides hydraulic oil pressure to the hydraulic cylinder assembly in the hydraulic station assembly. The regulating valve is installed on the output pipeline of the air-driven hydraulic pump to regulate the oil pressure of the hydraulic oil output by the air-driven hydraulic pump. The directional valve is a four-way ball valve, which is installed on the hydraulic oil pipeline connecting the hydraulic station assembly and each of the hydraulic cylinder assemblies. It is used to change the direction of hydraulic oil flow and realize the upward or downward movement of the cylinder axis. The switching valve is installed on the hydraulic oil pipeline connecting the hydraulic station assembly and each of the hydraulic cylinder assemblies, and is used to open and close the hydraulic oil pipeline.

[0010] According to the present invention, hydraulic cylinder assemblies of different specifications are set up to load preparative liquid chromatography columns of different diameters to ensure optimal loading parameters such as loading pressure and loading speed. The present invention proposes a universal preparative chromatography column packing station system that can pack all preparative chromatography columns in the laboratory, thereby improving equipment utilization and reducing operating costs.

[0011] This invention eliminates the need for an extension rod to change the diameter of the cylinder's central shaft, making it not only more convenient but also safer to use. Attached Figure Description

[0012] Figure 1 A schematic perspective view of a general-purpose preparative chromatography column packing station system according to one embodiment of the present invention; Figure 2 This schematic diagram shows a front view of a general-purpose preparative chromatography column packing station system according to one embodiment of the present invention. Figure 3 A schematic perspective view of a hydraulic station assembly according to one embodiment of the present invention; Figure 4 This is a schematic perspective view of a hydraulic cylinder assembly according to one embodiment of the present invention. Detailed Implementation

[0013] The present invention will now be discussed with reference to exemplary embodiments. It should be understood that the described embodiments are merely intended to enable those skilled in the art to better understand and thus implement the present invention, and are not intended to imply any limitation on the scope of the present invention.

[0014] As used herein, the term "comprising" and its variations are to be interpreted as open-ended terms meaning "including but not limited to". The term "based on" is to be interpreted as "at least partially based on". The terms "one embodiment" and "an embodiment" are to be interpreted as "at least one embodiment".

[0015] Figure 1 A schematic perspective view of a general-purpose preparative chromatography column packing station system according to one embodiment of the present invention; Figure 2 This schematic diagram shows a front view of a general-purpose preparative chromatography column packing station system according to one embodiment of the present invention. Figure 1 and Figure 2 As shown, in this embodiment, the general-purpose preparative chromatography column packing station system includes: a hydraulic station assembly 1, a hydraulic cylinder assembly 2, and a preparative liquid chromatography column 3; Hydraulic cylinder assemblies 2 of various specifications can be detachably installed on hydraulic station assembly 1; Hydraulic station component 1 is connected to each hydraulic cylinder component 2 through hydraulic oil pipelines, providing different hydraulic oil pressures to each hydraulic cylinder component 2. The preparative liquid chromatography column 3 includes a column cylinder 4 and a piston rod assembly 5; Multiple chromatographic column cylinders 4 are supported at intervals on the hydraulic station assembly 1, and multiple piston rod assemblies 5 are supported on each chromatographic column cylinder 4 respectively, with their top ends connected to the cylinder central shaft of each hydraulic cylinder assembly 2.

[0016] Furthermore, Figure 3 This is a schematic perspective view of a hydraulic station assembly according to one embodiment of the present invention. Figures 1 to 3 As shown, in this embodiment, the hydraulic station assembly 1 includes: a support frame 6, a first support plate 7, and a second support plate 8; The first support plate 7 is supported on the top of the support frame 6, and the first support plate 7 is provided with a first mounting groove 9 and a second mounting groove 10. Two hydraulic cylinder assemblies 2 of different specifications are detachably supported on the first mounting groove 9 and the second mounting groove 10, respectively. The second support plate 8 is supported at the bottom of the support frame 6 and is arranged at intervals from the first support plate 7; The column cylinders 4 of the two preparative liquid chromatography columns 3 are supported at intervals on the second support plate 8, and the piston rod assemblies 5 of the two preparative liquid chromatography columns 3 are supported on each column cylinder 4 respectively, and the top ends are connected to the cylinder central shaft of each hydraulic cylinder assembly 2.

[0017] Furthermore, Figure 4 This is a schematic perspective view of a hydraulic cylinder assembly according to one embodiment of the present invention. Figures 1 to 4As shown, in this embodiment, the hydraulic cylinder assembly 2 includes: a bracket 11 and a hydraulic cylinder 12; The support frame 11 includes: a third support plate 13, a column 14, and a fourth support plate 15; The third support plate 13 is provided with a U-shaped groove 16 corresponding to the first mounting groove 9 or the second mounting groove 10; Four columns 14 are evenly spaced and supported on the third support plate 13; The fourth support plate 15 is supported on top of multiple columns 14; The hydraulic cylinder 12 is supported on the fourth support plate 15, and its cylinder shaft 17 extends through the through hole on the fourth support plate 15 toward the third support plate 13. One end of the piston rod assembly 5 for preparing the liquid chromatography column 3 passes through the first mounting groove or the second mounting groove and the U-shaped groove 16 and connects to the column cylinder 4; The other end of the piston rod assembly 5 is connected to the central shaft 17 of the oil cylinder.

[0018] Furthermore, such as Figures 1 to 3 As shown, in this embodiment, the hydraulic station assembly 1 further includes: two pressure gauges 18, a regulating valve 19, a directional valve 20, and two switching valves 21; Pressure gauge 18 is used to monitor and display the pressure value of the hydraulic oil output by the pneumatic pump that provides hydraulic oil pressure to the hydraulic cylinder assembly 2 in the hydraulic station assembly 1. The regulating valve 19 is installed on the output pipeline of the air-driven hydraulic pump to regulate the oil pressure of the hydraulic oil output by the air-driven hydraulic pump. The directional valve 20 is a four-way ball valve, which is installed on the hydraulic oil pipeline connecting the hydraulic station assembly 1 and each hydraulic cylinder assembly 2. It is used to change the direction of hydraulic oil flow and realize the upward or downward movement of the cylinder axis. The switching valve 21 is installed on the hydraulic oil pipeline connecting the hydraulic station assembly 1 and each hydraulic cylinder assembly 2, and is used to open and close the hydraulic oil pipeline. For example... Figure 1 As shown, in this embodiment, the pressure gauge 18 and the knobs of the regulating valve 19, directional valve 20 and switching valve 21 are all integrated on the support frame 6.

[0019] In this embodiment, such as Figures 1 to 3 As shown, the hydraulic station assembly 1 also includes an emergency button 22, which has the functions of starting, stopping and emergency stopping of the hydraulic circuit.

[0020] According to the above-described scheme of this utility model, the universal preparative chromatography column packing station system of this utility model is equipped with two hydraulic cylinder assemblies 2 of different specifications. Hydraulic cylinder 1 is suitable for packing preparative liquid chromatography columns with diameters of 30mm and 50mm, while hydraulic cylinder 2 is suitable for packing preparative liquid chromatography columns with diameters of 80mm and 100mm. The design of the cylinder shaft and hydraulic circuit of each hydraulic cylinder must match the specifications of the preparative liquid chromatography column being packed.

[0021] For example, when packing a preparative liquid chromatography column with a diameter of 100 mm, the steps are as follows: 1. First, confirm that the two switching valves are in the correct position and ensure that the corresponding hydraulic oil lines of hydraulic station assembly 1 and hydraulic cylinder assembly 2 are connected; 2. Fix the piston rod assembly 5 of the 100mm preparative liquid chromatography column to the lower part of the cylinder shaft 17 with a fixing pin, place the column cylinder 4 on the second support plate 8 of the corresponding column packing station (hydraulic station assembly), and fix it with screws; 3. Calculate the required cylinder loading oil pressure and observe the value of pressure gauge 18, which is the pressure value of the hydraulic oil output by the air-driven hydraulic pump; slowly adjust the opening of regulating valve 19 so that the value of pressure gauge 18 is equal to the required cylinder loading oil pressure value. 4. Adjust the directional valve 20 to the position where the cylinder shaft 17 moves downward. Pull the emergency stop button 22 outward to start the hydraulic station assembly. The piston rod assembly 5 moves downward to expel air from the piston drain port until a stable homogenate flows out. Alternatively, the homogenate can be discharged from the bottom flange drain port until the homogenate is completely discharged. Continue to stabilize the packed column bed under pressure for more than 1 hour. The packed column bed is formed after the packed homogenate enters the chromatographic column, the homogenate is discharged and compacted by the column packing station, and the solid packing forms a stable packed column bed inside the chromatographic column. The shape is consistent with the chromatographic column and forms a cylindrical packing block.

[0022] 5. After locking the column top flange, the preparative liquid chromatography column can be removed from the hydraulic station assembly, completing the entire column loading operation.

[0023] If the column efficiency of a preparative liquid chromatography column deteriorates after a period of use, requiring replacement or repacking of the packing material, the packing material inside the column must first be removed. The steps are as follows: 1. Place the preparative liquid chromatography column on the corresponding second support plate 8, start the hydraulic station assembly, use the fixing pin to connect the piston rod assembly and the cylinder shaft, and then operate the hydraulic station assembly to slightly press down the cylinder shaft so that the screws on the column top flange can be easily loosened. 2. Slowly raise the central shaft of the hydraulic cylinder to release the pressure in the preparative liquid chromatography column; 3. Disconnect the piston rod assembly from the cylinder shaft, remove the column top flange, then remove the column bottom flange, place the container holding the packing material directly below the chromatographic column, and reconnect the piston rod assembly from the cylinder shaft. 4. Set a safe oil pressure value so that the central shaft of the oil cylinder drives the piston rod assembly to move downward, completely pushing out the packing in the cylinder, thus completing the packing unloading work.

[0024] According to the above-mentioned solution of this utility model, this utility model sets up hydraulic cylinder assemblies of different specifications to fill preparative liquid chromatography columns of different diameters to ensure the optimal filling pressure and filling speed and other filling parameters. The present invention proposes a universal preparative chromatography column packing station system that can pack all preparative chromatography columns in the laboratory, thereby improving equipment utilization and reducing operating costs.

[0025] This invention eliminates the need for an extension rod to change the diameter of the cylinder's central shaft, making it not only more convenient but also safer to use.

[0026] Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Although the utility model has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made to it in form and detail without departing from the scope defined by the claims of this utility model.

Claims

1. A general-purpose preparative chromatography column packing station system, characterized in that, include: Hydraulic station assembly (1), hydraulic cylinder assembly (2), and preparative liquid chromatography column (3); Various hydraulic cylinder assemblies (2) of different specifications can be detachably installed on the hydraulic station assembly (1). The hydraulic station assembly (1) is connected to each of the hydraulic cylinder assemblies (2) through hydraulic oil pipelines, providing different hydraulic oil pressures to each of the hydraulic cylinder assemblies (2); The prepared liquid chromatography column (3) includes a column cylinder (4) and a piston rod assembly (5). Multiple chromatographic column cylinders (4) are supported at intervals on the hydraulic station assembly (1), and multiple piston rod assemblies (5) are supported on each of the chromatographic column cylinders (4), with their top ends connected to the cylinder central shaft of each of the hydraulic cylinder assemblies (2).

2. The universal preparative chromatography column packing station system according to claim 1, characterized in that, The hydraulic station assembly (1) includes: a support frame (6), a first support plate (7), and a second support plate (8); The first support plate (7) is supported on the top of the support frame (6), and the first support plate (7) is provided with a first mounting groove (9) and a second mounting groove (10). The hydraulic cylinder assemblies (2) of different specifications are detachably supported on the first mounting slot (9) and the second mounting slot (10); The second support plate (8) is supported at the bottom of the support frame (6) and is arranged at a distance from the first support plate (7); The column cylinders (4) of the two preparative liquid chromatography columns (3) are supported at intervals on the second support plate (8), and the piston rod assemblies (5) of the two preparative liquid chromatography columns (3) are supported on each of the column cylinders (4), and their top ends are connected to the cylinder central shaft of each of the hydraulic cylinder assemblies (2).

3. The universal preparative chromatography column packing station system according to claim 2, characterized in that, The hydraulic cylinder assembly (2) includes: a bracket (11) and a hydraulic cylinder (12); The bracket (11) includes: a third support plate (13), a column (14) and a fourth support plate (15); The third support plate (13) is provided with a U-shaped groove (16) corresponding to the first mounting groove (9) or the second mounting groove (10). Multiple columns (14) are evenly spaced and supported on the third support plate (13); The fourth support plate (15) is supported on top of the plurality of columns (14); The hydraulic cylinder (12) is supported on the fourth support plate (15), and its cylinder shaft (17) extends through the through hole on the fourth support plate (15) toward the third support plate (13); One end of the piston rod assembly (5) of the prepared liquid chromatography column (3) passes through the first mounting groove or the second mounting groove and the U-shaped groove (16) and connects to the chromatography column (4); The other end of the piston rod assembly (5) is connected to the cylinder shaft (17).

4. The general-purpose preparative chromatography column packing station system according to any one of claims 1-3, characterized in that, The hydraulic station assembly (1) also includes: two pressure gauges (18), a regulating valve (19), a directional valve (20), and two switching valves (21). The pressure gauge (18) is used to monitor and display the pressure value of the hydraulic oil output by the pneumatic pump that provides hydraulic oil pressure to the hydraulic cylinder assembly (2) in the hydraulic station assembly (1); The regulating valve (19) is installed on the output pipeline of the gas-driven hydraulic pump to regulate the oil pressure of the hydraulic oil output by the gas-driven hydraulic pump. The directional valve (20) is a four-way ball valve, which is installed on the hydraulic oil pipeline connecting the hydraulic station assembly (1) and each of the hydraulic cylinder assemblies (2) to change the direction of hydraulic oil flow and realize the upward or downward movement of the cylinder axis. The switching valve (21) is installed on the hydraulic oil pipeline connecting the hydraulic station assembly (1) and each of the hydraulic cylinder assemblies (2) for opening and closing the hydraulic oil pipeline.

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