A method of preparing a chromatographic column

Abstract

The utility model relates to the technical field of chromatographic column, concretely is a kind of preparation chromatographic column, including column body and installation component;The end of column body has outer thread;Installation component includes end cap threadedly connected on outer thread, positioning ring coaxially arranged in the inside of column body, installation frame mutually butted with positioning ring, filter plate is arranged on the ring surface in installation frame, plug-in ring is coaxially arranged on installation frame, and sealing ring is located between end cap and column body;Positioning ring is provided with the circular groove of plug-in ring embedding, the inside of circular groove is provided with buffer pad, plug-in ring and buffer pad are mutually extruded to set, and the end face of end cap and column body mutually adheres.The utility model not only can be installed to filter plate, but also can improve the sealing property and stability of end cap and column pipe connection.

Description

Technical Field

[0001] This utility model relates to the field of chromatography column technology, and in particular to a preparative chromatography column. Background Technology

[0002] Preparative chromatography columns are specialized chromatographic columns used for the separation and purification of compounds. Their core function is to obtain sufficient quantities of target components from complex mixtures to meet experimental or production needs. Preparative chromatography columns can be classified into two main categories: packed columns and open tubular columns. They are generally made of glass or metal and come in shapes such as straight tubes, coils, and U-shaped tubes. The bottom is typically supported by three sets of supports.

[0003] Chinese Patent CN218980536U discloses a preparative chromatographic column, comprising a container, split retaining rings, an intermediate flange, an upper piston, a lower piston, a piston sealing ring, a hollow tube, packing material, a disc spring, a conical column, and locking bolts. The hollow tube is connected to the upper piston via internal and external threads and passes through a top cover. A disc spring is installed inside the container between the upper piston and the top cover. The upper and lower pistons are connected as a single unit by internal and external threads. A piston sealing ring is fitted around the lower piston. The conical column is placed in a conical hole in the lower piston. The locking bolt connects to the threaded hole inside the lower piston and presses against the upper conical column. The lower part of the container mates with the bottom flange, and the lower plane of the container engages with a sieve plate. The packing material is located in the internal space between the lower piston and the sieve plate inside the container. This preparative chromatographic column has advantages such as high efficiency, short separation and purification time, and good separation effect.

[0004] However, the above technical solution has the following shortcomings: the preparative chromatographic column is the same as the traditional preparative chromatographic column, which uses a thinner sealing ring to directly seal the column tube port and end cap. However, the sealing effect of this sealing method is poor, and the filter plate lacks a stable structure, making it inconvenient to install the filter plate. Utility Model Content

[0005] The purpose of this invention is to address the problems existing in the background technology by proposing a method for preparing chromatographic columns that not only facilitates the installation of filter plates but also improves the sealing and stability of the connection between the end cap and the column tube.

[0006] The present invention provides a chromatographic column preparation method, comprising a column body and an installation assembly. The end of the column body has an external thread. The installation assembly includes an end cap threaded onto the external thread, a positioning ring coaxially disposed inside the column body, a mounting frame abutting against the positioning ring, a filter plate disposed on the inner annular surface of the mounting frame, an insert ring coaxially disposed on the mounting frame, and a sealing ring located between the end cap and the column body. The positioning ring has an annular groove into which the insert ring is embedded, and a buffer pad is disposed inside the annular groove. The insert ring and the buffer pad are pressed against each other, and the end faces of the end cap and the column body are in contact with each other.

[0007] Preferably, the end cap has a cylindrical protrusion coaxially arranged inside, and the sealing ring is located at the edge of the protrusion.

[0008] Preferably, the end cap is provided with a central hole that gradually enlarges in a stepped shape.

[0009] Preferably, a connecting plate is provided on the column, and the end face of the end cap and the connecting plate fit together.

[0010] Preferably, the connecting plate is provided with three arc-shaped through holes arranged in a ring array, and the end cap is provided with multiple threaded holes arranged in a ring array. Fastening bolts are respectively installed through the three arc-shaped through holes, and the fastening bolts are threaded into the inside of the threaded holes.

[0011] Preferably, the end cap is provided with a support assembly, which includes three fixed cylinders arranged in a ring array on the end cap, a support rod threadedly connected to the fixed cylinders, and a gripping plate provided at the end of the support rod.

[0012] Preferably, the bottom surface of the gripping chassis is provided with an anti-slip layer.

[0013] Compared with the prior art, the present invention has the following beneficial technical effects:

[0014] This utility model, through its installation components, can pre-position and reinforce the filter plate during installation, facilitating the installation of the filter plate. At the same time, the included sealing ring, connecting plate, and fastening bolts fully ensure the sealing and stability of the connection between the end cap and the column. Moreover, it is simple and convenient to operate and has good practicality. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0016] Figure 2 This is a schematic diagram showing the disassembled structure of the column and end cap of this utility model;

[0017] Figure 3 This is a cross-sectional view showing the connection between the column and the end cap of this utility model.

[0018] Figure 4 for Figure 3 A magnified schematic diagram of the structure at point A in the middle.

[0019] Reference numerals: 1. Column; 2. Positioning ring; 3. Mounting frame; 4. Filter plate; 5. Insert ring; 6. Buffer pad; 7. Sealing ring; 8. End cap; 9. External thread; 10. Protrusion; 11. Connecting plate; 12. Arc-shaped through hole; 13. Fastening bolt; 14. Threaded hole; 15. Center hole; 16. Fixing cylinder; 17. Support rod; 18. Grip plate; 19. Anti-slip layer. Detailed Implementation

[0020] Example 1

[0021] like Figure 1 - Figure 4 As shown, this embodiment proposes a preparation of a chromatographic column, including a column body 1 and an installation assembly; the end of the column body 1 has an external thread 9.

[0022] The mounting assembly includes an end cap 8 threaded onto an external thread 9, a positioning ring 2 coaxially disposed inside a column 1, a mounting frame 3 abutting against the positioning ring 2, a filter plate 4 disposed on the inner annular surface of the mounting frame 3, an insert ring 5 coaxially disposed on the mounting frame 3, and a sealing ring 7 located between the end cap 8 and the column 1; the positioning ring 2 is provided with an annular groove into which the insert ring 5 is embedded, and a buffer pad 6 is provided inside the annular groove, the insert ring 5 and the buffer pad 6 are pressed against each other, the end cap 8 and the end faces of the column 1 are in contact with each other, and a cylindrical protrusion 10 is coaxially disposed inside the end cap 8, with the sealing ring 7 located at the edge of the protrusion 10.

[0023] In this embodiment, when installing the filter plate 4, the mounting frame 3 on the filter plate 4 is embedded into the port of the column 1 and pressed down so that the mounting frame 3 fits against the positioning ring 2. At the same time, the insert ring 5 is inserted into the annular groove of the positioning ring 2. The buffer pad 6 is used to compensate for the gap until the mounting frame 3 is flush with the end face of the column 1. Then, the sealing ring 7 is placed and snapped into the edge of the protrusion 10 inside the end cover 8. The end cover 8 is then screwed into the external thread 9 on the column 1 until the end cover 8 is completely connected to the column 1. At this time, the end cover 8 and the column 1 squeeze the sealing ring 7, and the mounting frame 3 fits against the protrusion 10. This not only provides pre-positioning and reinforcement for the installation of the filter plate 4, making it easier to install, but also provides good sealing performance. The operation is simple and convenient, and it has good practicality.

[0024] It should be noted that the bottom surface of the mounting frame 3 is provided with an L-shaped groove (not shown) to facilitate the removal of the filter plate 4 from the port of the column 1 through the mounting frame when disassembling and replacing the filter plate 4.

[0025] Example 2

[0026] like Figure 2 and Figure 3 As shown, this embodiment proposes a method for preparing a chromatographic column. Compared with Embodiment 1, in this embodiment, the column body 1 is provided with a connecting disk 11, the end face of the end cap 8 and the connecting disk 11 are fitted together, the connecting disk 11 is provided with three arc-shaped through holes 12 arranged in a ring array, the end cap 8 is provided with multiple threaded holes 14 arranged in a ring array, and fastening bolts 13 are respectively provided through the three arc-shaped through holes 12, and the fastening bolts 13 are threadedly connected to the inside of the threaded holes 14.

[0027] In this embodiment, after the end cap 8 is screwed onto the outer surface of the column 1 by the external thread 9 and the end face is in contact with the connecting plate 11, the three fastening bolts 13 are respectively passed through the three arc-shaped through holes 12 and aligned with the threaded holes 14 on the end cap 8 for threaded connection until the fastening bolts 13 abut against the connecting plate 11. This secondary reinforcement connection avoids the problem of gaps between the column 1 and the end cap 8 due to the deflection of the connection part between the end cap 8 and the external thread 9, thus improving the structural stability of the prepared chromatographic column. Furthermore, the structural design of the arc-shaped through holes 12 and the threaded holes 14 ensures that there are corresponding threaded holes 14 for the fastening bolts 13 to be screwed on regardless of the angle from which the end cap 8 is screwed on, improving the convenience of use.

[0028] Example 3

[0029] like Figure 3 As shown, in this embodiment, a chromatographic column is prepared. Compared with Embodiment 1, in this embodiment, the end cap 8 is provided with a central hole 15 that is progressively enlarged in a stepped shape.

[0030] In this embodiment, the central hole 15 has a stepped distribution with three or more steps, which can make the separation of the separated substances more efficient.

[0031] Example 4

[0032] like Figure 1 As shown, this embodiment proposes a method for preparing a chromatographic column. Compared with Embodiment 1, in this embodiment, the end cap 8 is provided with a support assembly. The support assembly includes three fixed cylinders 16 arranged in a ring array on the end cap 8, a support rod 17 threadedly connected to the fixed cylinders 16, and a gripping plate 18 provided at the end of the support rod 17. The bottom surface of the gripping plate 18 is provided with an anti-slip layer 19.

[0033] In this embodiment, the stability of the chromatographic column during use is ensured by the three-sided support of the support rod 17 and the gripping plate 18. The anti-slip layer 19 further increases the friction between the gripping plate 18 and the contact surface, thereby further ensuring stability. The fixed cylinder 16 allows the support rod 17 to be disassembled for transportation or storage.

[0034] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited thereto. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present invention.

Claims

1. A method for preparing a chromatographic column, characterized in that, include: A column (1) with external threads (9) at its end; The mounting assembly includes an end cap (8) threaded onto an external thread (9), a positioning ring (2) coaxially disposed inside a column (1), a mounting frame (3) abutting against the positioning ring (2), a filter plate (4) disposed on the inner annular surface of the mounting frame (3), a plug ring (5) coaxially disposed on the mounting frame (3), and a sealing ring (7) located between the end cap (8) and the column (1); the positioning ring (2) is provided with an annular groove into which the plug ring (5) is embedded, and a buffer pad (6) is provided inside the annular groove; the plug ring (5) and the buffer pad (6) are pressed against each other, and the end faces of the end cap (8) and the column (1) are in contact with each other.

2. The method for preparing a chromatographic column according to claim 1, characterized in that, The end cap (8) has a cylindrical protrusion (10) coaxially arranged inside, and the sealing ring (7) is located at the edge of the protrusion (10).

3. The method for preparing a chromatographic column according to claim 1, characterized in that, The end cap (8) is provided with a central hole (15) that is progressively enlarged in a stepped shape.

4. The method for preparing a chromatographic column according to claim 1, characterized in that, A connecting plate (11) is provided on the column (1), and the end face of the end cap (8) and the connecting plate (11) are in contact with each other.

5. A chromatographic column preparation method according to claim 4, characterized in that, The connecting plate (11) is provided with three arc-shaped through holes (12) arranged in a ring array, and the end cap (8) is provided with multiple threaded holes (14) arranged in a ring array. Fastening bolts (13) are respectively installed through the three arc-shaped through holes (12), and the fastening bolts (13) are threaded into the inside of the threaded holes (14).

6. A preparative chromatographic column according to claim 1, characterized in that, The end cap (8) is provided with a support assembly, which includes three fixed cylinders (16) arranged in a ring array on the end cap (8), a support rod (17) threadedly connected to the fixed cylinder (16), and a gripping plate (18) provided at the end of the support rod (17).

7. A chromatographic column preparation method according to claim 6, characterized in that, The bottom surface of the gripping chassis (18) is provided with an anti-slip layer (19).

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