A special retinal cryo-peeling stage and experimental equipment

Abstract

The utility model provides a kind of special stage of retinal low temperature stripping and experimental equipment, it is related to experimental apparatus field. Including stage body, the top of stage body is horizontal plane, and recess is set in its top;Ice water channel is set below recess, and ice water channel is through stage body. By setting the stage body of top surface level, and setting recess to place retina tissue, so that the stripping operation of retina can be stably carried out, and tissue is not easy to shift;Ice water channel is set for the flow of ice water mixture, keep the low temperature of stage body, since it is just below recess, and then cold quantity can be transmitted to recess faster, ice water mixture is directly replenished to keep low temperature continuously, and PBS solution is not needed to be pre-cooled frequently, so that liquid exchange is convenient and fast;Stage body can also support operator's both hands, improve operation stability.

Description

Technical Field

[0001] This utility model relates to the field of experimental instruments, specifically to a special stage and experimental equipment for low-temperature retinal detachment. Background Technology

[0002] Collecting rat / puppy retinas for Western blot and PCR molecular biology experiments is an important part of basic ophthalmological research. The experiments require retinal dissection at low temperatures to minimize enzymatic cleavage of tissue proteins and RNA, thereby improving detection efficiency. Retinal dissection in rats / puppies must be performed under a microscope, requiring real-time adjustment of the field of view and focus based on the eye's position; this demands a demanding experimental environment and is technically challenging.

[0003] The most convenient method for operating in a low-temperature environment is on ice. A simplified procedure typically involves removing the eyeball from a rat / small rat, placing it in a culture dish containing PBS solution, and carefully dissecting the retina under a microscope using micro-forceps and micro-scissors. To ensure low temperatures, the PBS can be pre-cooled, but this presents inconvenience due to the need for multiple solution changes and the PBS's tendency to warm up, affecting experimental results. A common improvement is to place the culture dish on ice, but this method has several drawbacks. For example, it's inconvenient to place a large foam box under the microscope, not only because it doesn't fit, but also because it hinders two-handed operation. Furthermore, if only a small amount of ice is placed, it melts very easily at room temperature. The melted ice causes the bottom of the culture dish to become uneven due to lack of support, resulting in focal plane imbalance and blurred vision under the microscope, causing significant inconvenience for the operator. Utility Model Content

[0004] The purpose of this invention is to provide a special stage and experimental device for low-temperature retinal detachment, which can maintain stable support for the retina at low temperatures, and is simple to operate and easy to change the solution.

[0005] The embodiments of this utility model are implemented as follows:

[0006] This application provides a special stage for retinal cryoablation, including a stage body, the top of which is horizontal and has a groove; an ice water channel is provided below the groove, and the ice water channel penetrates the stage body.

[0007] Furthermore, based on the aforementioned scheme, the platform body includes an integrally connected platform block and two support blocks, the two support blocks being respectively disposed at the bottom ends of the platform block, and the ice water channel being formed between the two support blocks.

[0008] Furthermore, based on the aforementioned scheme, the stage body is made of metal.

[0009] Furthermore, based on the aforementioned scheme, the height of the platform body is 2±0.5cm; the height of the ice water channel is 1±0.5cm.

[0010] Furthermore, based on the aforementioned scheme, the groove is circular.

[0011] Furthermore, based on the aforementioned scheme, the diameter of the groove is 1 cm and the depth of the groove is 0.5 cm.

[0012] An experimental apparatus includes a petri dish and a stage, the stage being placed inside the petri dish; the petri dish is filled with an ice-water mixture, the ice-water mixture at least partially submerging the ice-water channels; and the groove is filled with PBS solution.

[0013] Furthermore, based on the aforementioned scheme, a circular filter paper is placed in the groove.

[0014] Compared with the prior art, the embodiments of this utility model have at least the following advantages or beneficial effects:

[0015] This application utilizes a stage body with a horizontal top and a recessed area on the top for placing retinal tissue and PBS solution for retinal dissection. An ice-water channel, penetrating the stage body, allows ice water to flow through the stage body below the recess, maintaining the retinal tissue at a low temperature. The horizontal top of the stage body and the recessed area for retinal tissue ensure stable dissection and prevent tissue displacement. The ice-water channel facilitates the flow of the ice-water mixture, maintaining the stage body's low temperature. Because it is positioned directly below the recess, it rapidly transfers cold energy to the recess, allowing for continuous low-temperature maintenance with direct replenishment of the ice-water mixture. This eliminates the need for frequent pre-cooling of the PBS solution, making solution changes convenient and quick. Furthermore, the stage body supports the operator's hands, preventing instability caused by suspended operation. Attached Figure Description

[0016] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the overall structure of the special stage for retinal cryoablation according to an embodiment of the present invention;

[0018] Figure 2 This is a schematic diagram of the structure of an experimental device according to an embodiment of the present invention.

[0019] Icons: 1-Stage body, 11-Groove, 12-Ice water channel, 13-Stage block, 14-Support block, 2-Cultural dish. Detailed Implementation

[0020] The embodiments of this application will now be described in detail with reference to the accompanying drawings.

[0021] Please refer to Figures 1-2 The image shows a schematic diagram of the overall structure of the special stage for cryogenic retinal detachment.

[0022] This embodiment provides a special stage for retinal cryoablation, including a stage body 1. The top of the stage body 1 is horizontal and has a groove 11. An ice water channel 12 is provided below the groove 11 and penetrates the stage body 1.

[0023] The following will further describe a special stage for cryogenic retinal detachment in this exemplary embodiment.

[0024] In some embodiments, the top of the stage body 1 is horizontal, and a groove 11 is formed on its top for placing retinal tissue and PBS solution. This facilitates the positioning and dissection of the retinal tissue, ensuring that the ocular tissue remains moist and preventing retinal tissue displacement. An ice-water channel 12 is provided below the groove 11, penetrating the stage body 1. The ice-water channel 12 allows for the flow of an ice-water mixture, thereby maintaining the low temperature of the stage body 1. Since the groove 11 is located below the ice-water channel 12, the cold energy can be quickly conducted to the groove 11, thus ensuring the low-temperature environment of the groove 11. With this structural design, the stage body 1 can be placed inside a box or culture dish 2, which is then filled with an ice-water mixture. By continuously adding the ice-water mixture, the low-temperature environment of the groove 11 can be maintained, eliminating the need for frequent pre-cooling of the PBS solution and making solution changes convenient and quick. Furthermore, the horizontal stage body 1 can ensure the stability of the low-temperature peeling operation, and there is no need to worry about the ice water melting and causing the operation surface to be unstable; it is also convenient for two-handed operation, and the stage body can also support the operator's hands to avoid unstable operation caused by suspension.

[0025] In a preferred embodiment, the stage body 1 includes an integrally connected carrier block 13 and two support blocks 14. The carrier block 13 is rectangular, and the two support blocks 14 are respectively disposed at the bottom ends of the carrier block 13. The support blocks 14 are also rectangular, with horizontal bottoms for stable placement. When the stage body 1 is placed in the box or petri dish 2, the carrier block 13 is supported by the two support blocks 14 on both sides, ensuring that the plane remains stable and thus achieving stable operation of retinal cryogenic detachment. An ice-water channel 12 is formed between the two support blocks 14, making the bottom of the entire structure concave. The ice-water channel 12 is close to the groove 11, thereby ensuring that the ice-water mixture can flow freely under the stage body 1, thus enabling rapid cryogenic cooling of the groove 11.

[0026] As a preferred implementation, the stage body 1 is made of metal, specifically iron. The iron material can ensure that the stage body 1 is quickly cooled by ice.

[0027] In a preferred embodiment, the height of the stage body 1 is 2±0.5cm, and its length and width can be designed to fit the size of the petri dish 2, allowing it to be placed in an existing petri dish 2. The height of the ice-water channel 12 is 1±0.5cm, placing it close to the groove 11 and allowing for a larger flow of ice-water mixture, thus enabling rapid cooling.

[0028] In a preferred embodiment, the groove 11 is circular, similar to the shape of the petri dish 2, which facilitates tissue placement and avoids damage to the tissue. The diameter of the groove 11 is 1 cm and the depth of the groove 11 is 0.5 cm, which ensures that the tissue is contained while avoiding excessive depth that would make operation difficult.

[0029] This application embodiment also provides an experimental device, including a petri dish 2 and the aforementioned stage. The stage is placed inside the petri dish 2, which can be a conventionally sized petri dish. The petri dish 2 is filled with an ice-water mixture, which at least partially submerges the ice-water channel 12. Generally, a small gap is left for the ice-water to flow freely. The groove 11 is filled with a small amount of PBS solution, just enough to keep the retinal tissue moist. This allows the PBS solution to be pre-cooled or not. The ice-water mixture cools the stage body 1, avoiding frequent replacement of the PBS solution, which requires a long pre-cooling time, and also preventing the pre-cooled solution from warming up and affecting the low-temperature ablation of the tissue. The petri dish 2 can be filled with a large amount of ice-water mixture, making its warming up extremely slow. The ice-water mixture can be continuously replenished into the petri dish 2 to maintain a low-temperature environment, making the operation convenient and quick.

[0030] As a preferred embodiment, a circular filter paper is placed in the groove 11 to increase the friction of the tissue and facilitate the peeling operation.

[0031] Furthermore, unless otherwise explicitly specified or limited, the terms "installation" and "connection" in this application embodiment should be interpreted broadly. For example, "connection" can be a detachable connection or a non-detachable connection; it can be a direct connection or an indirect connection through an intermediate medium. The terms "upper," "lower," "left," "right," "inner," "outer," and "side," etc., are merely for reference to the direction in the accompanying drawings or the usual placement of the product during use. They are only for clearly describing this application and do not indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. They should not be construed as limitations on this application. The terms "first," "second," etc., are only used for distinguishing descriptions and should not be construed as indicating or implying relative importance; "multiple" refers to at least two. In this application embodiment, the limitations on relative positional relationships such as parallel, perpendicular, and aligned are all relative to the current technological level and are not absolutely strict limitations. Slight deviations are allowed; approximations of parallel, perpendicular, and aligned are all acceptable. For example, "A and B are parallel" means that A and B are parallel or approximately parallel, and the angle between A and B can be between 0 degrees and 10 degrees.

[0032] The above are only some embodiments and implementation methods of this application. The protection scope of this application is not limited thereto. In the absence of conflict, the embodiments and features in the embodiments of this application can be combined with each other. Any combination of features in different embodiments is also within the protection scope of this application. Any changes or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this application should be included within the protection scope of this application.

Claims

1. A special stage for cryogenic retinal detachment, characterized in that, The device includes a platform body, the top of which is horizontal and has a groove. An ice water channel is provided below the groove and runs through the platform body. The platform body includes an integrally connected loading block and two support blocks. The two support blocks are respectively located at the bottom ends of the loading block, and the ice water channel is formed between the two support blocks.

2. The special stage for retinal cryo-detachment according to claim 1, characterized in that, The stage body is made of metal.

3. The stage for cryogenic retinal detachment according to claim 2, characterized in that, The height of the platform body is 2±0.5cm; the height of the ice water channel is 1±0.5cm.

4. The special stage for retinal cryo-detachment according to claim 1, characterized in that, The groove is circular.

5. The stage for cryogenic retinal detachment according to claim 4, characterized in that, The groove has a diameter of 1 cm and a depth of 0.5 cm.

6. An experimental apparatus, characterized in that, The invention includes a petri dish and a stage as described in any one of claims 1-5, the stage being placed inside the petri dish; the petri dish is filled with an ice-water mixture, the ice-water mixture at least partially submerging the ice-water channels; and the groove is filled with a PBS solution.

7. The experimental apparatus according to claim 6, characterized in that, A circular filter paper is placed inside the groove.

Images