Nucleic acid purification column temperature control protection device

Abstract

The utility model discloses nucleic acid purification column temperature control protection device belongs to nucleic acid purification equipment field, including main body shell, the upper end rotation of main body shell is installed with sealing cover, is provided with temperature control cavity in main body shell, the left end of main body shell is installed with heating sheet subassembly, heating sheet subassembly and the left side of temperature control cavity are pasted, the right end of main body shell is installed with semiconductor refrigeration sheet, semiconductor refrigeration sheet and the right side of temperature control cavity are pasted, the right end fixed mounting of semiconductor refrigeration sheet has the cooling fan, through the temperature control cavity in main body shell, can carry out stable temperature control to nucleic acid purification column, and the cooperation of heating sheet subassembly and semiconductor refrigeration sheet can realize the flexible adjustment to the temperature in temperature control cavity, satisfy the demand of different experiment to temperature, and through the setting of limiting component, can carry out the location fixed to nucleic acid purification column, avoid its in temperature control process and take place the shaking, improve temperature control accuracy.

Description

Technical Field

[0001] This utility model relates to the field of nucleic acid purification equipment technology, specifically a nucleic acid purification column temperature control and protection device. Background Technology

[0002] In biological experiments and molecular diagnostics, nucleic acid purification is a crucial step. As a core component in this process, the performance of the nucleic acid purification column directly affects the purity and yield of the final nucleic acid. The nucleic acid purification process of oligonucleotides is quite sensitive to temperature. Temperature fluctuations may affect the structure and properties of oligonucleotides, thereby affecting the purification effect and quality.

[0003] However, in practice, the efficiency and stability of nucleic acid purification columns are often affected by changes in ambient temperature. In particular, under high or low temperature conditions, the enzyme activity in the nucleic acid purification column may decrease, leading to a decrease in purification efficiency and even affecting the accuracy of subsequent experimental results.

[0004] Therefore, this invention provides a temperature control and protection device for nucleic acid purification columns to solve the above problems. Utility Model Content

[0005] This invention provides a temperature control and protection device for nucleic acid purification columns, aiming to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution: It includes a main body shell, a sealing cover rotatably mounted on the upper end of the main body shell, a temperature control cavity disposed inside the main body shell, a heating element assembly installed inside the left end of the main body shell, the heating element assembly being fitted to the left side of the temperature control cavity, a semiconductor cooling chip installed inside the right end of the main body shell, the semiconductor cooling chip being fitted to the right side of the temperature control cavity, a cooling fan fixedly mounted on the right end of the semiconductor cooling chip, and a limit component disposed inside the main body shell.

[0007] As a preferred technical solution of this application, the limiting component includes an adapter plate, which is fixedly installed in the temperature control cavity. Multiple sets of through holes are arrayed in the adapter plate, and multiple sets of movable grooves are circumferentially formed in the adapter plate at the inner wall of the multiple sets of through holes. A first spring is installed in each of the multiple sets of movable grooves. Telescopic rods are movably installed at the openings of the multiple sets of main body shells, and a limiting plate is installed at one end of each of the multiple sets of telescopic rods.

[0008] As a preferred technical solution of this application, the lower corners of the main body shell are all wrapped with anti-collision strips.

[0009] As a preferred technical solution of this application, a flap is rotatably installed on both the left and right sides of the temperature control cavity. Four sets of connecting rods are movably inserted into each of the two sets of flaps. A pressure plate is fixedly installed at the lower end of the four sets of connecting rods installed in the two sets of flaps. A return spring is sleeved at the lower end of each of the four sets of connecting rods installed in the two sets of flaps. The two sets of pressure plates and the adapter plate are arranged parallel to each other vertically.

[0010] As a preferred technical solution of this application, the upper side of the adapter plate is provided with multiple sets of hole position marking plates, and the multiple sets of hole position marking plates are located on the right side of the multiple sets of through holes.

[0011] As a preferred technical solution of this application, multiple sets of grooves are provided on the lower side of both sets of pressure plates, and the grooves on the lower side of the two sets of pressure plates are directly above the multiple sets of through holes.

[0012] The beneficial effects of this utility model are as follows: the temperature control cavity inside the main body shell can stably control the temperature of the nucleic acid purification column; the combined use of the heating element assembly and the semiconductor cooling element can flexibly adjust the temperature inside the temperature control cavity to meet the temperature requirements of different experiments; and the setting of the limiting component can limit and fix the nucleic acid purification column to prevent it from shaking during the temperature control process, thereby improving the temperature control accuracy. Attached Figure Description

[0013] Figure 1 This is a front view structural diagram of the present utility model;

[0014] Figure 2 This is a front view of the cross-sectional structure of this utility model;

[0015] Figure 3 This is a front view cross-sectional diagram of the temperature control cavity in this utility model;

[0016] Figure 4 This is a top view cross-sectional diagram of the adapter plate in this utility model;

[0017] Figure 5 for Figure 4 A magnified structural diagram at point A.

[0018] In the picture:

[0019] 1. Main body shell; 2. Sealing cover; 3. Anti-collision strip; 4. Temperature control cavity; 5. Heating element assembly; 6. Semiconductor cooling chip; 7. Cooling fan; 8. Adapter plate; 9. Through hole; 10. Movable groove; 11. First spring; 12. Telescopic rod; 13. Limiting plate; 14. Flip plate; 15. Connecting rod; 16. Pressure plate; 17. Return spring; 18. Hole position marking plate. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] This utility model provides a temperature control and protection device for nucleic acid purification columns, such as... Figure 1-5 As shown, the nucleic acid purification column temperature control protection device includes a main shell 1, a sealing cover 2 rotatably installed on the upper end of the main shell 1, a temperature control cavity 4 disposed inside the main shell 1, a heating element assembly 5 installed inside the left end of the main shell 1, the heating element assembly 5 being attached to the left side of the temperature control cavity 4, a semiconductor cooling chip 6 installed inside the right end of the main shell 1, the semiconductor cooling chip 6 being attached to the right side of the temperature control cavity 4, a cooling fan 7 fixedly installed on the right end of the semiconductor cooling chip 6, and a limit component disposed inside the main shell 1.

[0022] The heating element assembly 5 generates heat through an external power supply to heat the temperature control cavity 4, while the semiconductor cooling element 6 achieves cooling or heating functions through changes in current, thereby regulating the temperature of the temperature control cavity 4. The cooling fan 7 can accelerate the heat dissipation efficiency of the semiconductor cooling element 6 and improve the temperature control accuracy. In use, the operator can open the sealing cover 2, insert the nucleic acid purification column into the limiting component inside the temperature control cavity 4, and fix it in place by the limiting component.

[0023] The limiting component includes an adapter plate 8, which is fixedly installed inside the temperature control cavity 4. Multiple sets of through holes 9 are arrayed inside the adapter plate 8. Multiple sets of movable grooves 10 are circumferentially formed inside the adapter plate 8 at the inner wall of the multiple sets of through holes 9. A first spring 11 is installed in each of the multiple sets of movable grooves 10. Telescopic rods 12 are movably installed at the openings of the multiple sets of main body shells 1. A limiting plate 13 is installed at one end of each set of telescopic rods 12.

[0024] When the nucleic acid purification column is inserted into the through hole 9 of the adapter plate 8, the first spring 11 in the multiple sets of movable grooves 10 is squeezed and generates elastic force, which pushes the telescopic rod 12 to drive the limiting plate 13 to clamp and limit the nucleic acid purification column, making the nucleic acid purification column more stable during the temperature control process and avoiding shaking, which would affect the temperature control effect.

[0025] The lower corners of the main body shell 1 are all wrapped with anti-collision strips 3.

[0026] The anti-collision strip 3 can act as a buffer when the device is hit, preventing damage to the device and extending its service life.

[0027] Flip plates 14 are rotatably installed on both sides of the temperature control cavity 4. Four sets of connecting rods 15 are movably inserted into each of the two sets of flip plates 14. Pressure plates 16 are fixedly installed at the lower ends of the four sets of connecting rods 15 installed in the two sets of flip plates 14. Return springs 17 are sleeved at the lower ends of the four sets of connecting rods 15 installed in the two sets of flip plates 14. The two sets of pressure plates 16 are arranged parallel to the adapter plate 8.

[0028] When the limiting plate 13 clamps and limits the nucleic acid purification column, it drives the two sets of flaps 14 to rotate. The two sets of flaps 14 push the two sets of pressure plates 16 downward through the connecting rod 15. The pressure plates 16 compress the reset spring 17, so that the two sets of pressure plates 16 press down and fix the upper end of the nucleic acid purification column.

[0029] The upper side of the adapter plate 8 is provided with multiple sets of hole position marking plates 18, which are located to the right of multiple sets of through holes 9.

[0030] The well labeling plate 18 clearly marks the sample number corresponding to each well of the purification column, avoiding sample confusion.

[0031] Multiple grooves are provided on the lower side of both pressure plates 16, and the grooves on the lower side of the two pressure plates 16 are directly above the multiple through holes 9.

[0032] The rotation of the two sets of flip plates 14 drives the two sets of pressure plates 16 to rotate synchronously, so that the two sets of pressure plates 16 are parallel to the upper side of the adapter plate 8. This allows the upper end of the nucleic acid purification column inserted into the multiple sets of through holes 9 to be locked in the multiple sets of grooves opened on the lower side of the pressure plate 16, thereby further limiting and fixing the upper end of the nucleic acid purification column and improving the overall stability of the fixation.

[0033] Working principle:

[0034] In use, the operator first opens the sealing cap 2, then inserts the hole position marking plate 18 on the corresponding adapter plate 8 of the nucleic acid purification column into the corresponding through hole 9. At this time, the first spring 11 in the multiple sets of movable grooves 10 is compressed and generates elastic force, pushing the telescopic rod 12 to drive the limiting plate 13 to clamp and limit the nucleic acid purification column. At the same time, the limiting plate 13 drives the two sets of flip plates 14 to rotate. The two sets of flip plates 14 push the two sets of pressure plates 16 to rotate through the connecting rod 15. Under the action of multiple sets of reset springs 17, the two sets of pressure plates 16 press down and fix the upper end of the nucleic acid purification column. The upper side of the plate 16 is parallel to the upper side of the adapter plate 8, thereby locking the upper end of the nucleic acid purification column, which is inserted into multiple sets of through holes 9, into multiple sets of grooves opened on the lower side of the pressure plate 16, further limiting and fixing the upper end of the nucleic acid purification column. Then, the operator supplies power to the heating element assembly 5 through an external power source. The heating element assembly 5 generates heat to heat the temperature control cavity 4, or the semiconductor cooling chip 6 achieves cooling or heating functions through changes in current, thereby regulating the temperature of the temperature control cavity 4. During the temperature control process, the setting of the cooling fan 7 can accelerate the heat dissipation efficiency of the semiconductor cooling chip 6 and improve the temperature control accuracy.

[0035] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A nucleic acid purification column temperature control and protection device, comprising a main body shell (1), characterized in that: A sealing cover (2) is rotatably installed on the upper end of the main body shell (1). A temperature control cavity (4) is provided inside the main body shell (1). A heating element assembly (5) is installed inside the left end of the main body shell (1). The heating element assembly (5) is attached to the left side of the temperature control cavity (4). A semiconductor cooling chip (6) is installed inside the right end of the main body shell (1). The semiconductor cooling chip (6) is attached to the right side of the temperature control cavity (4). A cooling fan (7) is fixedly installed on the right end of the semiconductor cooling chip (6). A limit component is provided inside the main body shell (1).

2. The nucleic acid purification column temperature control and protection device according to claim 1, characterized in that: The limiting component includes an adapter plate (8), which is fixedly installed inside the temperature control cavity (4). Multiple sets of through holes (9) are arrayed inside the adapter plate (8). Multiple sets of movable grooves (10) are circumferentially opened inside the adapter plate (8) at the inner wall of the multiple sets of through holes (9). A first spring (11) is installed in each of the multiple sets of movable grooves (10). Telescopic rods (12) are movably installed at the openings of the multiple sets of main body shells (1). A limiting plate (13) is installed at one end of each of the multiple sets of telescopic rods (12).

3. The nucleic acid purification column temperature control and protection device according to claim 1, characterized in that: The lower corners of the main body shell (1) are all wrapped with anti-collision strips (3).

4. The nucleic acid purification column temperature control and protection device according to claim 2, characterized in that: Flip plates (14) are rotatably installed on both sides of the temperature control cavity (4). Four sets of connecting rods (15) are movably inserted into each of the two sets of flip plates (14). Pressure plates (16) are fixedly installed at the lower ends of the four sets of connecting rods (15) installed in the two sets of flip plates (14). Return springs (17) are sleeved at the lower ends of the four sets of connecting rods (15) installed in the two sets of flip plates (14). The two sets of pressure plates (16) are arranged parallel to the adapter plate (8) vertically.

5. The nucleic acid purification column temperature control and protection device according to claim 2, characterized in that: The upper side of the adapter plate (8) is provided with multiple sets of hole position marking plates (18), and the multiple sets of hole position marking plates (18) are located on the right side of multiple sets of through holes (9).

6. The nucleic acid purification column temperature control and protection device according to claim 4, characterized in that: Multiple grooves are provided on the lower side of both sets of pressure plates (16), and the grooves on the lower side of both sets of pressure plates (16) are directly above the multiple through holes (9).

Images