A flushing tank and flushing device for HE staining
The HE staining rinsing tank with a bottom-up buffer plate and diversion hole structure solves the problems of difficult-to-control water flow impact and uneven staining, achieving uniform rinsing and integrity protection of the slides, and meeting different experimental needs.
Patent Information
- Application Number
- CN202522096964.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-29
- Publication Date
- 2026-08-25
- Estimated Expiration
- 2035-09-29
AI Technical Summary
In existing HE staining techniques, the impact force of the water flow during rinsing is difficult to control precisely, leading to section damage and uneven staining. Furthermore, the rinsing time is difficult to match with specific staining requirements, affecting pathological diagnosis and research.
A rinsing tank for HE staining was designed, which adopts a bottom-up buffer plate and diversion hole structure, combined with a regulating valve and timer, to achieve precise control of water flow rate and time, ensuring uniform rinsing and section integrity.
It effectively reduces the probability of section damage, improves staining uniformity and washing quality, meets different experimental needs, and protects the integrity and quality of sections.
Smart Images

Figure CN224673326U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of slide staining technology, and in particular to a rinsing tank and rinsing device for HE staining. Background Technology
[0002] In pathological and histological research and practice, hematoxylin and eosin (HE) staining, as a classic and widely used staining technique, plays a crucial role. HE staining, also known as hematoxylin-eosin staining, is an indispensable and key step in paraffin sectioning and is one of the most commonly used staining methods. This staining method clearly reveals the structure and morphology of tissue cells, providing important evidence for subsequent pathological diagnosis and research.
[0003] In the complex process of hematoxylin and eosin (HE) staining, rinsing is a crucial and indispensable step. After staining the sections in hematoxylin and eosin solutions, rinsing is necessary to remove excess dye and ensure the sections achieve the desired staining effect. However, under current technological conditions, there are several unresolved problems when rinsing sections.
[0004] First, the water flow impact force and uniformity during rinsing are extremely difficult to control precisely. Due to the lack of effective adjustment and control methods, the water flow impact force may be excessive. When a large water impact force acts directly on the slide, the slide itself is relatively fragile and easily breaks. This breakage not only destroys the integrity of the slide but may also lead to the loss of important tissue information, affecting subsequent observation and analysis.
[0005] Secondly, uneven impact is also a common problem. If the water flow is unevenly distributed during rinsing, the impact force on different locations on the section will vary. This results in inconsistent removal of excess dye across different areas of the section, leading to uneven staining. Some areas may be stained too darkly, while others may be stained too lightly, failing to accurately represent the true morphology and structure of the tissue cells.
[0006] Furthermore, existing rinsing methods cannot precisely control the rinsing time according to actual needs. Different tissue samples and different staining requirements often require different rinsing times to achieve the best staining results. However, current technology cannot meet this need, making it difficult to match the rinsing time with the specific staining situation. This inevitably affects the staining effect and quality of the final section, causing certain difficulties for pathological diagnosis and research. Utility Model Content
[0007] In view of this, the purpose of this utility model is to overcome the shortcomings in related technologies. This utility model provides a rinsing tank and rinsing device for HE staining.
[0008] This utility model provides the following technical solution: A rinsing tank for HE staining includes a tank body and a buffer plate.
[0009] The bottom of the tank is connected to a water injection seat, which is connected to a water supply device via a pipe. The buffer plate is horizontally installed in the tank and is used to place a clamping seat containing slices to be rinsed. As the water level rises, the water injected into the tank through the water injection seat can successively submerge the buffer plate and the slices on the clamping seat, thereby rinsing the slices.
[0010] As a further improvement to the above technical solution, the buffer plate is provided with multiple diversion holes evenly distributed throughout.
[0011] As a further improvement to the above technical solution, the diameter of the diversion hole is d, and the value range of d is: 1.8mm≤d≤2.4mm.
[0012] As a further improvement to the above technical solution, the distance between the buffer plate and the bottom of the trough is A, and the depth of the trough is B. The values of A and B satisfy the following range: B / 4≤A≤B / 3.
[0013] As a further improvement to the above technical solution, a support step corresponding to the buffer plate is provided on the inner side wall of the tank, and the support step can provide support for the buffer plate that is horizontally set in the tank.
[0014] As a further improvement to the above technical solution, the water injection seat is provided with a regulating valve, which is used to regulate the flow rate of water flowing through the water injection seat into the tank.
[0015] As a further improvement to the above technical solution, a timer is also provided on the water injection base.
[0016] As a further improvement to the above technical solution, the upper opening edge of the groove is provided with a bent portion that bends inward toward the inside of the groove.
[0017] As a further improvement to the above technical solution, both the tank and the buffer plate are made of stainless steel.
[0018] This utility model also provides a flushing device, including the flushing tank for HE staining as described above.
[0019] Compared with related technologies, the beneficial effects of this utility model are: The HE staining rinsing tank provided by this utility model, in actual operation, when rinsing stained sections, firstly, a clamp holding the stained sections must be prepared. The clamp holding is used to securely hold the stained sections, ensuring that the sections will not move randomly during subsequent rinsing. The clamp holding is then placed on a buffer plate inside the tank. The buffer plate can, to a certain extent, impede and regulate the impact force generated when the water rises, providing a gentler rinsing environment for the sections.
[0020] After placing the clamping base, place the entire tank into the flushing pool of the flushing device. The flushing pool can catch water overflowing during the flushing process. Next, connect the water injection seat at the bottom of the tank to the water supply equipment through a suitable pipe.
[0021] After completing the above preparations, start the water supply equipment. The equipment begins operation, and water is injected into the tank from the bottom through the water inlet. As the equipment continues to run, the water volume in the tank gradually increases, and the liquid level slowly rises. During this process, the liquid level gradually submerges the buffer plate and then the slices placed on the clamping base. The water flows from bottom to top, rinsing the slices; this rinsing method is significantly different from the traditional top-down rinsing process.
[0022] As the water level in the tank continues to rise, eventually overflowing the top opening of the tank, it will naturally spill into the flushing pool. The flushing pool has a drainage function; this overflowing water will be drained away through the pool's drainage system, thus maintaining the stability and cleanliness of the entire flushing environment.
[0023] During the rinsing process described above, the water level in the tank gradually rises as the water volume increases. Compared to existing top-down rinsing processes, this bottom-up rinsing method provides a gentler and more even impact on the sections. In traditional top-down rinsing, the water flow directly impacts the sections, easily causing excessive localized stress and damage. However, in the rinsing tank of this invention, the water flow rises slowly from the bottom, evenly distributing the impact force on the sections, effectively reducing the probability of damage during rinsing and better protecting the integrity and quality of the stained sections.
[0024] Furthermore, the upward rinsing method ensures a horizontal rinsing surface. Combined with the buffer plate's ability to restrain and regulate the rising water, the water flow can more evenly cover all parts of the slice during its ascent. Whether it's the center or the edges of the slice, it receives the same level of rinsing, thus ensuring uniform rinsing across all parts of the slice and significantly improving the rinsing quality.
[0025] Furthermore, by adjusting the water delivery speed of the water supply equipment, operators can more easily and flexibly adjust the water injection rate of the tank according to actual needs. If rapid rinsing of the sections is required, the water delivery speed of the equipment can be appropriately increased to accelerate the increase of water volume and the rise of the liquid level in the tank; if a higher degree of rinsing precision is required, the water delivery speed can be reduced to allow the water flow to rise slowly, thus making the rinsing process more gentle and ensuring that the rinsing efficiency and quality of the sections meet different experimental requirements.
[0026] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, preferred embodiments are described below in detail with reference to the accompanying drawings. Attached Figure Description
[0027] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.
[0028] Figure 1 This invention provides a schematic diagram of the rinsing tank for HE staining from one perspective in one embodiment of the present invention. Figure 2 This invention provides a schematic diagram of the rinsing tank for HE staining from another perspective in one embodiment of the present invention. Figure 3 This diagram shows another perspective view of the rinsing tank for HE staining in one embodiment of the present invention.
[0029] Explanation of key component symbols: 100-Tank body; 110-Water injection seat; 120-Regulating valve; 130-Bend; 140-Support step; 200-Buffer plate; 210-Diverter hole. Detailed Implementation
[0030] The embodiments of this utility model are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this utility model, and should not be construed as limiting this utility model.
[0031] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.
[0032] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.
[0033] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.
[0034] In this utility model, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.
[0035] Combination Figure 1 , Figure 2 As shown, an embodiment of this utility model provides a rinsing tank for HE staining, including a tank body 100 and a buffer plate 200.
[0036] The bottom of the tank 100 is connected to a water injection seat 110, which is connected to a water supply device via a pipe. The buffer plate 200 is horizontally installed inside the tank 100, and a clamping seat containing slices to be rinsed is placed on the buffer plate 200. As the water level rises, the water injected into the tank 100 through the water injection seat 110 can successively submerge the buffer plate 200 and the slices on the clamping seat, thereby rinsing the slices.
[0037] The HE staining rinsing tank provided in this embodiment, in actual operation, when rinsing stained sections, firstly, a holder containing the stained sections must be prepared. The holder is used to securely hold the stained sections, ensuring that the sections will not move randomly during subsequent rinsing. The holder is then placed on the buffer plate 200 inside the tank body 100. The buffer plate 200 can, to a certain extent, impede and regulate the impact force generated when the water rises, providing a gentler rinsing environment for the sections.
[0038] After placing the clamping seat, place the entire tank 100 into the flushing pool of the flushing device. The flushing pool can catch the water overflowing during the flushing process. Next, connect the water injection seat 110, which is connected to the bottom of the tank 100, to the water supply equipment through a suitable pipe.
[0039] After completing the above preparations, start the water supply equipment. The equipment begins operation, and water is injected into tank 100 from the bottom through the water inlet 110. As the equipment continues to operate, the water volume in tank 100 gradually increases, and the liquid level slowly rises. During this process, the liquid level gradually submerges the buffer plate 200, and then the slices placed on the clamping seat. The water flows from bottom to top, rinsing the slices; this rinsing method is significantly different from the traditional top-down rinsing process.
[0040] As the water level in tank 100 continues to rise and reaches a certain point, the water that overflows the top opening of tank 100 will naturally spill into the flushing pool. The flushing pool has a drainage function, and the overflowing water will eventually be drained away through the flushing pool's drainage system, thus maintaining the stability and cleanliness of the entire flushing environment.
[0041] During the rinsing process described above, the water level in tank 100 gradually rises as the water volume increases. Compared to existing top-down rinsing processes, this bottom-up rinsing method provides a gentler and more even impact on the sections. In traditional top-down rinsing, the water flow directly impacts the sections, easily causing excessive local stress and damage. However, in the rinsing tank of this invention, the water flow rises slowly from the bottom, evenly distributing the impact force on the sections, effectively reducing the probability of damaging them and better protecting the integrity and quality of the stained sections.
[0042] Furthermore, the upward rinsing method ensures a horizontal rinsing surface. Combined with the buffer plate 200's ability to restrain and regulate the rising water, the water flow can more evenly cover all parts of the slice during its ascent. Whether it's the center or the edges of the slice, it receives the same level of rinsing, thus ensuring uniform rinsing across all parts of the slice and significantly improving the rinsing quality.
[0043] Furthermore, by adjusting the water delivery speed of the water supply equipment, operators can more easily and flexibly adjust the water injection rate of the tank 100 according to actual needs. If rapid rinsing of the sections is required, the water delivery speed of the equipment can be appropriately increased to accelerate the increase of water volume and the rise of the liquid level in the tank 100; if a higher degree of rinsing precision is required, the water delivery speed can be reduced to allow the water flow to rise slowly, thus making the rinsing process more gentle and ensuring that the rinsing efficiency and quality of the sections meet different experimental requirements.
[0044] In some specific embodiments, the buffer plate 200 has multiple evenly distributed diversion holes 210. When the water in the tank 100 gradually increases under the action of the water conveyance equipment, and the liquid level rises until it submerges the buffer plate 200, these diversion holes 210 begin to play a crucial role. The originally upward-flowing water, upon encountering the buffer plate 200, will not directly impact the slices placed in the clamping seat above the buffer plate 200 with a large impact force, but will instead be diverted through the various diversion holes 210. During the process of the water flow passing through the diversion holes 210, its flow direction and speed change, and the originally relatively concentrated water flow is dispersed into multiple fine water flows, thereby achieving effective buffering and regulation of the rising water volume.
[0045] This buffering mechanism has several important implications. Firstly, it further reduces the impact of water flow on the slides. In traditional rinsing methods, the water flow directly impacts the slides, easily causing excessive localized stress, leading to slide damage or uneven staining. However, through the diversion effect of the buffer plate 200, the impact of the water flow on the slides is greatly weakened, making the force on the slides more uniform and gentle during rinsing, effectively reducing the risk of slide damage and ensuring the integrity and quality of the slides.
[0046] On the other hand, the design of the diversion holes 210 also ensures the uniformity of rinsing the sections. Because the water flow is evenly distributed through multiple diversion holes 210, the water flow distribution within the tank 100 is more balanced. Whether the section is located at the center or edge of the buffer plate 200, it receives water rinsing with approximately the same intensity and flow rate. This avoids over-rinsing in some areas and under-rinsing in others, ensuring that all parts of the section receive sufficient and uniform rinsing, thus improving the rinsing effect and staining quality.
[0047] In some specific embodiments, the diameter of the diversion hole 210 is d, and the value of d is within the range of 1.8mm ≤ d ≤ 2.4mm. When the diameter of the diversion hole 210 is within this range, it can effectively ensure the diversion and mitigation of the rising water. As the water surges upward from the bottom of the tank 100 and gradually submerges the buffer plate 200, the water flow is dispersed into multiple fine streams due to the presence of the diversion hole 210. If the diameter of the diversion hole 210 is too small, for example, much smaller than 1.8mm, the water flow will be greatly obstructed when passing through these tiny holes, causing the water flow velocity to drop sharply, or even the water flow to be unable to pass smoothly. This not only fails to achieve normal buffering and regulation of the rising water, but may also cause uneven water distribution within the tank 100, affecting the rinsing effect on the stained sections.
[0048] When the diameter of the diversion orifice 210 is within the reasonable range of 1.8mm - 2.4mm, the water flow can pass through each diversion orifice 210 relatively smoothly. Simultaneously, the speed and direction of the water flow are appropriately adjusted, making the originally turbulent and concentrated upward water flow relatively gentle and uniform. This gentle water flow can act more gently on the stained sections placed in the clamp above the buffer plate 200, avoiding excessive impact on the sections and effectively protecting the integrity of the sections and the staining quality.
[0049] At the same time, this diameter range also avoids unnecessary obstruction to the upward flow of water. If the diameter of the diversion orifice 210 is too large, for example, much larger than 2.4 mm, then the buffering and regulating effect of the buffer plate 200 on the water flow will be greatly reduced. When the water flows through the large-diameter diversion orifice 210, it will hardly be significantly dispersed or buffered, and will still surge upward with a large impact force. This would fail to achieve the original design purpose of the buffer plate 200, and would not be able to ensure that the buffer plate 200 achieves the ideal buffering and regulating effect on the water flow.
[0050] In some specific embodiments, the distance between the buffer plate 200 and the bottom of the tank 100 is A, and the depth of the tank 100 is B. The values of A and B satisfy the following range: B / 4 ≤ A ≤ B / 3. Maintaining this specific distance between the buffer plate 200 and the bottom of the tank plays an important role. When the water initially enters the tank 100, its flow is unstable due to the water being injected from the water supply equipment, resulting in some turbulence. If the distance between the buffer plate 200 and the bottom of the tank is too small, or even if it is directly against the bottom, this turbulence will directly pass through the buffer plate 200 and rinse the stained sections placed on it before it has been adequately buffered and stabilized. The uneven impact direction and force of the turbulence will cause significant differences in the rinsing force received by different parts of the sections, thus seriously affecting the uniformity of rinsing and resulting in inconsistent staining effects that fail to meet the accuracy requirements of the experiment. By controlling the distance between the buffer plate 200 and the bottom of the tank within the range of B / 4 to B / 3, the water flow has enough space and time to initially diffuse and stabilize after entering the tank 100, and the turbulent flow can gradually become relatively stable. After further adjustment by the buffer plate 200, the slices can be rinsed with a more uniform water flow, effectively ensuring the uniformity of rinsing.
[0051] On the other hand, considering the overall structure and ease of use of the tank 100, since the buffer plate 200 maintains the aforementioned reasonable distance from the tank bottom, there is naturally a larger distance between the buffer plate 200 and the tank top. In practical applications, there are many types of stained sections, and the sizes of different types of sections vary, as do the sizes of the clamps used to place the sections. This larger distance between the buffer plate 200 and the tank top allows clamps of different sizes to be easily placed on the buffer plate 200. Furthermore, regardless of the size of the clamp, the sections placed on the buffer plate 200 can be completely immersed in the water flow within the tank 100. This ensures that during the rinsing process, the entire section is fully covered and rinsed by the water flow, preventing any sections from being exposed outside the water flow and unable to be effectively rinsed. This greatly ensures the reliability of section rinsing and improves the applicability and practicality of the entire HE staining rinsing tank.
[0052] In some specific embodiments, the inner sidewall of the tank 100 is provided with a support step 140 corresponding to the buffer plate 200. The support step 140 can provide support for the buffer plate 200 which is horizontally set in the tank 100, making it convenient to remove and clean the buffer plate 200 regularly or replace it as needed, thus ensuring the reliability of this embodiment.
[0053] like Figure 3As shown, in some specific embodiments, the water injection seat 110 is equipped with a regulating valve 120. The regulating valve 120 has the function of flexibly adjusting the water flow rate, and it can precisely control the water flow rate entering the tank 100 through the water injection seat 110. In actual operation, when the water supply equipment is started to inject water into the tank 100, the water flow first reaches the water injection seat 110. At this time, by operating the regulating valve 120, the water flow rate can be dynamically adjusted according to actual needs. For example, when it is necessary to rinse some fragile and easily damaged stained slides, the regulating valve 120 can be appropriately reduced to decrease the water flow rate, making the water flow into the tank 100 more gentle; while for some slides with more attached impurities and requiring stronger rinsing force, the regulating valve 120 can be increased to increase the water flow rate.
[0054] By adjusting the flow rate of the water flowing through the water inlet seat 110, the water injection speed into the tank 100 can be precisely controlled. This is because the water injection speed directly depends on the amount of water entering the tank 100 per unit time, and the control of the flow rate by the regulating valve 120 determines the amount of water passing through the water inlet seat 110 per unit time. When the regulating valve 120 increases the flow rate, the amount of water entering the tank 100 per unit time increases, and the water injection speed increases; conversely, when the regulating valve 120 slows down the flow rate, the amount of water entering the tank 100 per unit time decreases, and the water injection speed decreases.
[0055] This adjustment of the water injection speed ultimately allows for flexible control of the rinsing speed of the slides. During rinsing, different stained slides may require different rinsing speeds to achieve the best rinsing effect. If the rinsing speed is too fast, the impact of the water flow on the slides is too great, which may cause slide displacement, damage, or uneven staining; if the rinsing speed is too slow, it may not be able to effectively remove impurities and excess dye from the slide surface, affecting the staining quality. By adjusting the water injection speed through the regulating valve 120, it can be ensured that the slides receive a uniform and appropriate water flow impact during rinsing. A uniform water flow impact ensures that all parts of the slide are thoroughly rinsed, avoiding over-rinsing or under-rinsing in certain areas; an appropriate water flow impact can be precisely controlled according to the characteristics of the slides, achieving a good rinsing effect without damaging the slides.
[0056] In some specific embodiments, a timer is also provided on the water injection base 110. During the actual operation of injecting water into the tank 100 to rinse the stained sections, the rinsing time is a crucial influencing factor. Different stained sections, due to differences in their material, thickness, degree of staining, and surface impurities, require different optimal rinsing times. If the rinsing time is too short, excess dye and impurities on the section surface may not be sufficiently removed, leading to uneven staining or inaccurate subsequent analytical results; while if the rinsing time is too long, it not only wastes water resources and increases experimental costs but may also cause unnecessary damage to the sections, such as softening the section texture or destroying its structure.
[0057] When the operator turns on the water supply equipment and begins filling the tank 100 with water, a timer set on the water injection seat 110 can be started simultaneously. The timer will record the flushing duration in precise time units, such as seconds or minutes. During the flushing process, the operator can check the time displayed on the timer at any time to monitor the flushing progress in real time.
[0058] By observing and analyzing the time recorded by the timer, operators can easily and accurately record the rinsing time of the slides. This recorded data serves as an important reference for subsequent experiments, helping researchers summarize the optimal rinsing time range for different types of stained slides. At the same time, the timer also provides strong support for operators to strictly control the rinsing time. Operators can, according to the preset rinsing time, promptly turn off the water supply device and stop water injection when the timer reaches the corresponding time, ensuring that each stained slide receives just the right amount of rinsing within the specified time.
[0059] In some specific embodiments, the upper opening edge of the tank 100 is provided with a bent portion 130 that curves inward into the tank 100; when the bent portion 130 is not provided, the upper opening edge of the tank 100 is usually a relatively sharp right-angled edge. In actual use, operators may need to move the tank 100 for various reasons, such as changing the experimental position or cleaning and maintaining the tank 100. When operators move the tank 100, their hands or other parts of their bodies can easily and accidentally touch the sharp upper opening edge of the tank 100. Due to the sharp edges of the right-angled edge, once touched, it is very likely to cause cuts, scratches, or other injuries to the operator's skin. This will not only cause physical pain to the operator, but may also affect the normal progress of the experiment, or even cause the experiment to be interrupted.
[0060] By incorporating the bend 130 that curves inward into the tank 100, the edges of the tank 100 achieve a smooth transition. The originally sharp right angles are replaced by gentle curves, making the entire edge smooth and flat. This prevents injury from sharp edges when an operator moves the tank 100, even if their hands or other body parts accidentally come into contact with its edge. The smooth edges effectively distribute the pressure generated upon contact, reducing the risk of skin injury.
[0061] In some specific embodiments, both the tank 100 and the buffer plate 200 are made of stainless steel. Stainless steel possesses many significant characteristics that perfectly suit the needs of rinsing tanks. From a corrosion resistance perspective, in laboratory environments, rinsing tanks often come into contact with various chemical reagents and solutions, such as dyes used in staining processes and acid / alkali solutions used for cleaning. These chemicals are highly corrosive. If the tank 100 and buffer plate 200 are made of ordinary materials, they are prone to rusting, corrosion, and deformation after prolonged contact with these chemicals. This not only affects the appearance of the rinsing tank but also reduces its structural strength and service life. It may even lead to impurities falling off during the rinsing of slides, contaminating the slides and affecting the accuracy of experimental results. Stainless steel, on the other hand, has excellent corrosion resistance, remaining stable in various chemical environments and not easily corroded. This ensures that the tank 100 and buffer plate 200 can be used for a long time without damage, providing a reliable and stable rinsing environment for experiments.
[0062] In terms of strength and hardness, stainless steel possesses high strength and hardness. During flushing, the water flow exerts a certain impact force on the tank 100 and the buffer plate 200, especially when adjusting the water flow rate and injection speed, as the magnitude of the impact force varies. If the material's strength and hardness are insufficient, the tank 100 and the buffer plate 200 may deform under the long-term impact of the water flow, leading to a decrease in the buffering effect of the buffer plate 200 and a deterioration in the sealing performance of the tank 100, thereby affecting the normal operation of the entire flushing system. The high strength and hardness of stainless steel effectively resist the impact of the water flow, ensuring the structural stability of the tank 100 and the buffer plate 200, and maintaining good performance at all times.
[0063] Furthermore, stainless steel offers excellent cleanability and hygiene. In laboratories, the cleanliness and hygiene of experimental equipment are extremely important to prevent cross-contamination and ensure the reliability of experimental results. The smooth surface of stainless steel makes it resistant to dirt and bacteria buildup. After each use, it can be easily cleaned by rinsing with water or wiping with a regular cleaning agent, leaving no harmful residues and meeting the stringent standards for equipment cleanliness and hygiene in laboratories.
[0064] An embodiment of this utility model also provides a rinsing device, including a rinsing tank for HE staining as described in the above embodiment. The rinsing tank for HE staining is placed in the rinsing pool of the rinsing device for use. The rinsing device has all the beneficial effects of the rinsing tank for HE staining, which will not be described in detail here.
[0065] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.
[0066] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.
Claims
1. A rinsing tank for HE staining, characterized in that, include: The tank (100) has a water injection seat (110) connected to its bottom, and the water injection seat (110) is connected to the water conveying equipment through a pipe; A buffer plate (200) is horizontally installed in the tank (100), and a clamping seat containing a water-washing slice is placed on the buffer plate (200). Water injected into the tank (100) through the water injection seat (110) rises with the liquid level, and can successively submerge the buffer plate (200) and the slices on the clamping seat, thereby rinsing the slices.
2. The rinsing tank for HE staining according to claim 1, characterized in that, The buffer plate (200) has multiple diversion holes (210) evenly distributed on it.
3. The rinsing tank for HE staining according to claim 2, characterized in that, The diameter of the diversion hole (210) is d, and the value of d is within the range of 1.8mm≤d≤2.4mm.
4. The rinsing tank for HE staining according to claim 2, characterized in that, The distance between the buffer plate (200) and the bottom of the trough (100) is A, and the depth of the trough (100) is B. The values of A and B satisfy the following range: B / 4≤A≤B / 3.
5. The rinsing tank for HE staining according to claim 4, characterized in that, The inner wall of the trough (100) is provided with a support step (140) corresponding to the buffer plate (200), and the support step (140) can provide support for the buffer plate (200) which is horizontally set in the trough (100).
6. The rinsing tank for HE staining according to any one of claims 1 to 5, characterized in that, The water injection base (110) is provided with a regulating valve (120), which is used to regulate the flow rate of water flowing through the water injection base (110) into the tank (100).
7. The rinsing tank for HE staining according to claim 6, characterized in that, The water injection base (110) is also equipped with a timer.
8. The rinsing tank for HE staining according to any one of claims 1 to 5, characterized in that, The upper opening edge of the groove (100) is provided with a bent portion (130) that bends inward toward the inside of the groove (100).
9. The rinsing tank for HE staining according to any one of claims 1 to 5, characterized in that, Both the trough (100) and the buffer plate (200) are made of stainless steel.
10. A flushing device, characterized in that, Includes a rinsing tank for HE staining as described in any one of claims 1 to 9.