Sample preparation cartridge with aggregation function

Abstract

The sample preparation box with the aggregation function comprises a sampling spoon, a containing part and a cover part; the sampling spoon comprises a rotating part and a spoon part; the rotating part is fixedly connected with the spoon part; the cover part comprises a rotating connecting part; the rotating part is connected with the rotating connecting part; the containing part comprises an A cavity and a B cavity; a filter partition plate is arranged between the A cavity and the B cavity; in a closed state, the cover part covers an opening part of the A cavity, and the spoon part is in the A cavity; a downward recess is arranged at the bottom of the B cavity, and the downward recess is used for sample aggregation and enrichment; the sample can be broken, uniformly distributed and rotated, and large impurities can be filtered and separated; by arranging the downward recess interval in the enrichment cavity, the target component is enriched in a concentrated place, and the target component is convenient for being sucked by a pipette tip.

Description

Technical Field

[0001] This application belongs to the field of detection and analysis technology, and specifically relates to reagent kits and carrier devices used in the field of automated detection. Background Technology

[0002] Before biological samples can be tested and analyzed, they need to undergo processing procedures corresponding to the specific testing method. Different testing methods have different requirements and procedures for sample pretreatment. Raw samples typically require specialized operations such as dilution, staining, centrifugation, and concentration. These preparation operations usually need to be performed by testing personnel according to standardized procedures.

[0003] Therefore, both human and veterinary hospitals need to fully automate the testing process, minimizing the steps that require human intervention.

[0004] In the process of fecal testing, sample preparation is completed using automated instruments, and a sampling preparation kit has emerged that combines sample collection and sample mixing.

[0005] In the preparation of fecal test samples, some tests require crushing and rotating to separate the target formed elements from the sample; other tests require aggregating formed elements with low concentrations, such as fecal parasite eggs. In these tests, large particles need to be separated first, while smaller particles are continuously enriched, allowing the fecal parasite eggs to settle and accumulate at the bottom.

[0006] The technical problem that needs to be solved is how to complete the crushing, rotation, separation of large impurities, and enrichment of target components in an automated sample preparation kit. Summary of the Invention

[0007] This application proposes an automated sample preparation box that supports sample crushing, rotation for uniform distribution, and filtration to separate large impurities. By setting a recessed area in the enrichment chamber, the target component is enriched in a concentrated area, making it easier for the pipette tip to aspirate the target component.

[0008] This application proposes an automated sample preparation kit that supports sample crushing, uniform rotation and distribution, and filtration to separate large impurities. During the preparation process, liquid is added through a liquid inlet to improve stirring efficiency. By adding absorbent blocks, water absorption promotes the separation of formed particles during the preparation process, and finally, the absorbent blocks absorb the liquid to achieve an enrichment effect.

[0009] A sample preparation box with aggregation function includes: a sampling spoon, a receiving part, and a cover; the sampling spoon includes a rotating part and a spoon part; the rotating part and the spoon part are fixedly connected; the cover includes a rotating connecting part; the rotating part and the rotating connecting part are connected; the receiving part includes cavity A and cavity B; a filter partition is provided between cavity A and cavity B; in the closed state, the cover covers the opening of cavity A, and the spoon part is in cavity A; the bottom of cavity B includes a downwardly recessed part, which is used for sample aggregation and enrichment.

[0010] Alternatively, the downward concave portion at the bottom of cavity B may be an inverted cone shape.

[0011] Alternatively, the cover may also include an inlet / outlet hole; in the closed state, the cover covers the openings of cavity A and cavity B, and the inlet / outlet hole corresponds to the opening of cavity B, for the entry and exit of substances in cavity B.

[0012] Alternatively, the filter baffle may include an inclined portion that is inclined toward cavity B, thereby increasing the opening area of ​​cavity A.

[0013] It is possible that the bottom of cavity A is higher than the bottom of cavity B.

[0014] It is possible that the bottom of cavity A and part of cavity B are on a continuous inclined surface, with the lowest point of the inclined surface at the bottom of cavity B.

[0015] Alternatively, the cover may also include a liquid filling hole; in the closed state, the liquid filling hole is used to add reagents, dyes, or diluents to cavity A.

[0016] It may also include a C cavity, which is used to place the absorbent block; the C cavity is located on the other side of the B cavity away from the A cavity.

[0017] It may also include a C filter partition; the B cavity and the C cavity are separated by the C filter partition.

[0018] It is possible that the C filter plate includes a filter screen, wherein the mesh size of the filter screen is greater than 240 mesh and less than 400 mesh.

[0019] One of the technical advantages of the above-mentioned solution is that the bottom of cavity B includes a downward-curved section, which is used for sample aggregation and enrichment. Cavity B has a smaller cross-sectional area, allowing the target analyte to aggregate and deposit in the dispersed suspension within this space, facilitating subsequent sampling. Furthermore, the enrichment level increases closer to the bottom.

[0020] One of the technical effects of the above-mentioned technical solution is that the downward concave part at the bottom of cavity B is inverted cone-shaped, which helps the target detection material to accumulate in this space and facilitates the subsequent sample collection.

[0021] One of the technical effects of the above-mentioned technical solution is that the filter plate includes a filter screen, and the number of meshes of the filter screen can be greater than 150 meshes and less than 250 meshes, which facilitates the entry of target objects within a set size range into cavity B.

[0022] One of the technical effects of the above-mentioned technical solution is that the inlet and outlet holes correspond to the opening of cavity B, which is used for the entry and exit of substances in cavity B, facilitating the input and output of samples.

[0023] One of the technical effects of the above-mentioned technical solution is that adding liquid through the liquid inlet hole improves the efficiency of stirring.

[0024] One of the technical effects of the above-mentioned technical solution is that by adding water-absorbing blocks, water absorption promotes the formation of diverted liquids during the preparation process, and finally absorbs the liquid to achieve the enrichment effect.

[0025] One of the technical effects of the above-mentioned technical solution is that the bottom of cavity A is higher than the bottom of cavity B, which facilitates the enrichment of the target substance.

[0026] One of the technical effects of the above-mentioned technical solution is that the C-cavity, which is used to place the water-absorbing block, is conducive to further enrichment.

[0027] One of the technical effects of the above-mentioned technical solution is that the filter plate C includes a filter screen with a mesh size greater than 240 and less than 400, which facilitates the entry of target objects within a set size range into the cavity B without them being absorbed by the absorbent block. Attached Figure Description

[0028] Figure 1 This is a three-dimensional view of Embodiment 1; Figure 2 This is a three-dimensional exploded view of the sampling spoon and lid in Example 1; Figure 3 This is a three-dimensional exploded view schematic diagram of Example 1; Figure 4 This is a schematic diagram of the centerline cross-sectional view of Example 1; Figure 5 This is a three-dimensional view schematic diagram of Embodiment 2; Figure 6 This is a schematic diagram of an exploded view of Example 2; Figure 7 This is a schematic diagram of a three-dimensional sectional view of Embodiment 2; Figure 8 This is a three-dimensional top view of the receiving part of Embodiment 2; Figure 9 This is a schematic diagram of a perspective view of Embodiment 3; Figure 10 This is a schematic diagram of an exploded view of Example 3; Figure 11This is a schematic diagram of the centerline sectional view of Example 3; Figure 12 This is a schematic diagram of the exploded perspective view of Example 3. Detailed Implementation

[0029] The content of this application will be further described in detail below with reference to the accompanying drawings. It should be noted that the following description is of preferred embodiments of this application and does not constitute any limitation on this application. The description of the preferred embodiments is merely an explanation of the general principles of this application. The use of terms such as "first," "second," and "A," "B" in this application is for ease of explanation only and does not represent a temporal or spatial order. The combinations of letters and numbers "S," "M," and "H" in this application are also for ease of explanation, and their specific meanings are determined by the specific content they refer to.

[0030] like Figure 1 The perspective view of Embodiment 1 includes: a sampling spoon, a receiving part 0110, and a cover part 0120.

[0031] like Figure 2 Example 1: An exploded perspective view of the sampling spoon and the cover 0120. The sampling spoon 0210 includes a rotating part 0211 and a spoon part 0212; the rotating part 0211 and the spoon part 0212 are fixedly connected; the cover 0120 includes a rotating connecting part 0220; the rotating part 0211 and the rotating connecting part 0220 are connected.

[0032] like Figure 3 The exploded perspective view of Embodiment 1 shows that the receiving part includes cavity A 0310 and cavity B 0320; a filter partition 0340 is provided between cavity A and cavity B; in the closed state, the cover 0120 covers the opening of cavity A, and the spoon 0220 is in cavity A.

[0033] like Figure 4 The schematic diagram of the midline cross-section of Example 1 shows that the bottom of cavity B includes a downward recess 0410, which is used for sample aggregation and enrichment. The downward recess at the bottom of cavity B is inverted conical in shape.

[0034] like Figure 3 The cover also includes an inlet / outlet hole 0350; in the closed state, the cover covers the openings of cavity A 0310 and cavity B, and the inlet / outlet hole corresponds to the opening of cavity B 0320 for the entry and exit of substances in cavity B.

[0035] like Figure 5 The three-dimensional view of Example 2 shows that the cover 0510 also includes a liquid filling hole 0520; in the closed state, the liquid filling hole is used to add reagents, dyes or diluents to cavity A.

[0036] like Figure 6The exploded view of Embodiment 2 shows that the filter baffle 0610 includes an inclined portion 0611, which is inclined toward cavity B 0620 and expands the opening area of ​​cavity A 0630.

[0037] like Figure 7 The schematic diagram of the three-dimensional sectional view of Example 2 shows that the bottom of cavity A (0710) is higher than the bottom of cavity B (0620). This facilitates the flow of the target analyte from cavity A to cavity B. Figure 7 The bottom of cavity A includes an upward protrusion, which is used to prevent the sample from accumulating in the center of the bottom. The upward protrusion is columnar (0750).

[0038] like Figure 8 The schematic diagram of the three-dimensional top view of the receiving part of Embodiment 2 shows that the columnar object 0750 is provided with fins 0810 on the outside.

[0039] like Figure 9 A schematic diagram of a three-dimensional view of Example 3; as shown Figure 10 A schematic diagram of the exploded view of Embodiment 3; Embodiment 3 includes: a sampling spoon 0910, a receiving part 0920, and a cover part 0930; a filter partition 0953 is provided between cavity A 0951 and cavity B 0952.

[0040] like Figure 11 A schematic diagram of the centerline cross-section of Embodiment 3; the bottom of part of cavity A 0951 and cavity B 0952 is on a continuous inclined surface 1110, and the low point 1111 of the inclined surface is at the bottom of cavity B.

[0041] like Figure 11 It also includes cavity C 1130, which is used to place the absorbent block; cavity C is located on the other side of cavity B away from cavity A.

[0042] like Figure 12 The device includes: a sampling spoon, a receiving part, and a cover; the sampling spoon includes a rotating part and a spoon part; the rotating part and the spoon part are fixedly connected; the cover includes a rotating connecting part; the rotating part and the rotating connecting part are connected; the receiving part includes cavity A and cavity B; a filter baffle 0953 is provided between cavity A and cavity B; the cover also includes a liquid inlet 1250; in the closed state, the liquid inlet is used to add reagents, staining agents or diluents to cavity A 0951; in the closed state, the cover covers the opening of cavity A, and the spoon part is in cavity A.

[0043] like Figure 11 The liquid filling hole 1250 is connected to the inlet / outlet hole 1251; the connection facilitates processing.

[0044] like Figure 11 , Figure 12It includes a C cavity 1130, which is used to place the water-absorbing block 1210; the C cavity is located on the other side of the B cavity away from the A cavity.

[0045] With the absorbent block, during sample preparation, as the absorbent block absorbs water and the liquid level drops, it is necessary to replenish the dilution liquid through the liquid addition hole. The absorbent block also promotes the flow of liquid from cavity A to cavity B, facilitating the accumulation of formed elements in cavity B.

[0046] Figure 12 It also includes a C filter partition 1280; the B cavity and the C cavity are separated by the C filter partition. The C filter partition facilitates the installation of the filter screen and can also restrict the spatial position of the water absorption block.

[0047] Figure 12 The C filter baffle includes a filter screen 1281 with a mesh size greater than 240 and less than 400. This filter screen is densely packed to prevent the target analyte from being drawn into the C cavity by the absorbent block.

[0048] While this application has been described and illustrated with reference to preferred embodiments and several alternatives, it is not intended to be limited to the specific descriptions herein. Other alternatives or equivalent components may also be used to practice this application.

Claims

1. A sample preparation kit with aggregation function, characterized in that, include: Sampling spoon, receiving part, lid; The sampling spoon includes a rotating part and a spoon part; the rotating part and the spoon part are fixedly connected. The cover includes a rotatable connecting part; the rotatable part is connected to the rotatable connecting part; The receiving part includes cavity A and cavity B; a filter partition is provided between cavity A and cavity B; In the closed state, the cover covers the opening of cavity A, and the spoon is inside cavity A; The bottom of cavity B includes a downward recess, which is used for sample aggregation and enrichment.

2. The sample preparation kit with aggregation function according to claim 1, characterized in that, The downward concave portion at the bottom of cavity B is inverted conical in shape.

3. The sample preparation kit with aggregation function according to claim 1, characterized in that, The cover also includes an inlet / outlet hole; In the closed state, the cover covers the openings of cavity A and cavity B, and the inlet / outlet hole corresponds to the opening of cavity B, for the entry and exit of substances in cavity B.

4. The sample preparation kit with aggregation function according to claim 1, characterized in that, The filter baffle includes an inclined portion that is inclined toward cavity B, thereby increasing the opening area of ​​cavity A.

5. The sample preparation kit with aggregation function according to claim 1, characterized in that, The bottom of cavity A is higher than the bottom of cavity B.

6. The sample preparation kit with aggregation function according to claim 5, characterized in that, The bottom portions of cavity A and cavity B are on a continuous inclined surface, with the lowest point of the inclined surface at the bottom of cavity B.

7. The sample preparation kit with aggregation function according to claim 1, characterized in that, The cover also includes a liquid filling hole; In the closed state, the liquid inlet is used to add reagents, stains, or diluents to cavity A.

8. The sample preparation kit with aggregation function according to claim 1, characterized in that, It also includes a C cavity, which is used to place the absorbent block; the C cavity is located on the other side of the B cavity away from the A cavity.

9. The sample preparation kit with aggregation function according to claim 8, characterized in that, It also includes a C filter partition; the B cavity and the C cavity are separated by the C filter partition.

10. The sample preparation kit with aggregation function according to claim 9, characterized in that, C. The filter plate includes a filter screen with a mesh size greater than 240 and less than 400.

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