Biological culture vessel

Abstract

The utility model relates to a kind of biological culture containers, including shell, the shell is provided with air inlet, exhaust, the bottom of the shell is provided with vent pipe, the vent pipe is communicated with the gas source outside, for providing the gas required for reaction in shell, the vent pipe is immersed in culture solution in shell.Gas can be mixed with culture solution sufficiently, improve the reaction speed of gas and culture solution, temperature control is carried out using heat exchanger before gas is passed into culture solution, improve the heat exchange efficiency, more suitable for large volume culture container.

Description

Technical Field

[0001] This utility model relates to the field of biotechnology, and in particular to a biological culture container. Background Technology

[0002] Bioculture containers (also known as disposable bioreactor bags or cell culture bags) are flexible, sterile containers used for cell culture, microbial fermentation, or biopharmaceutical production, and are widely used, especially in mammalian cell culture, vaccine production, and personalized medicine. During bioculture, a suitable gaseous environment (such as oxygen and carbon dioxide) needs to be provided within the container. Currently, gas is often introduced into the top space of the container shell, especially in swing-type bioreactors. However, this aeration method is suitable for small-volume bioculture containers. When the culture shell volume exceeds 20L, it is difficult to meet the gas supply required for cell growth, especially for the cultivation of highly aerobic microorganisms. Furthermore, to reduce the metabolic temperature during microbial or cell culture, water baths are usually installed on the outer wall of the container for cooling. For larger culture containers, this results in unsatisfactory cooling rates and low heat transfer efficiency. Additionally, the water baths require a chiller, making the structure complex and costly. Summary of the Invention

[0003] To address the aforementioned problems, this utility model provides a biological culture container, comprising a shell, an air inlet and an air outlet, and a vent pipe at the bottom of the shell, which is connected to an external air source to supply the gas required for the reaction into the shell. The vent pipe is immersed in the culture medium inside the shell.

[0004] Furthermore, the side wall of the vent pipe is provided with multiple air outlets.

[0005] Furthermore, the ventilator is a straight pipe, a ring pipe, or a serpentine pipe.

[0006] Furthermore, the air inlet is connected to the air source, and a heat exchanger is provided between the air source and the air inlet. The heat exchanger is used to heat or cool the gas.

[0007] Furthermore, the heat exchanger directly or indirectly exchanges heat with the gas passing through it.

[0008] Furthermore, the heat exchanger is equipped with heating / cooling plates or heating / cooling medium fluid pipelines.

[0009] Furthermore, a gas sterilization device is connected to the pipeline between the air inlet and the air source.

[0010] Furthermore, the housing also includes a feed inlet and a sampling port.

[0011] Furthermore, the housing also includes a sensor interface.

[0012] Furthermore, the gas source provides air, oxygen, or carbon dioxide gas.

[0013] The present invention allows gas from the gas source to be introduced into the culture medium through the aforementioned gas outlet, enabling thorough mixing between the gas and the culture medium and improving the reaction rate. This design is more suitable for large-volume culture containers. Temperature control via a heat exchanger before the gas is introduced into the culture medium improves heat exchange efficiency. Compared to devices such as water baths, this design is smaller, lower in cost, and allows for faster adjustment. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of a biological culture container structure; Figure 2 This is a schematic diagram of a straight pipe structure for the venting tube; Figure 3 This is a schematic diagram of a loop-type vent pipe. Figure 4 This is a schematic diagram of a serpentine vent tube structure. Detailed Implementation

[0015] See Figure 1-2 The biological culture container of this utility model includes a shell 1, on which are provided a feed inlet 2, an air inlet 3, an exhaust outlet 4, a sampling port 5, a sensor interface 6, etc., for providing culture medium and gas into the shell, as well as for exhausting gas and sampling from the shell, and for installing sensors to monitor the reaction status inside the shell.

[0016] A vent pipe 7 is provided at the bottom of the shell 1. The vent pipe is connected to an external gas source to provide the gas required for the reaction inside the shell. There can be multiple vent pipes, and multiple vent holes are provided on the side wall of each vent pipe. During operation, the vent pipe is immersed in the culture medium inside the shell, and the gas from the gas source is introduced into the culture medium through the vent holes. In this way, the gas can be fully mixed with the culture medium. Compared with introducing gas above the culture medium, this can increase the contact area between the gas and the culture medium, making it more suitable for large-volume culture containers.

[0017] The cross-section of the vent pipe can be circular, semi-circular, square, etc. The path of the vent pipe can be a straight pipe (…). Figure 2 ), ring pipe ( Figure 3 ), serpentine tube ( Figure 4 The longer the aeration tube, the more thorough its contact with the culture medium, and the larger the contact area. Furthermore, multiple aeration tubes can be used (in...). Figure 1 (Two examples are given below).

[0018] To address the issue of temperature regulation using water bath tubes in existing technologies, this invention incorporates a heat exchanger 8 between the gas source and the ventilation pipe. As the gas passes through the heat exchanger 8, it heats or cools the gas according to the required culture temperature. The heat exchanger can employ direct heat exchange (e.g., gas passing through an electric heating wire, heating plate, or cooling plate) or indirect heat exchange (e.g., using a heat exchange tube with a fluid flowing inside and the gas flowing outside). Through this method, the gas is temperature-regulated before being introduced into the culture medium, and the agitation between the gas and the culture medium improves heat exchange efficiency. Compared to water bath temperature control devices, this invention is smaller and offers higher adjustment precision.

[0019] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.

Claims

1. A biological culture container, comprising a shell (1), wherein the shell (1) is provided with an air inlet (3) and an air outlet (4), characterized in that, The bottom of the shell (1) is provided with a vent pipe (7), which is connected to an external gas source and is used to provide the gas required for the reaction to the shell. The vent pipe (7) is immersed in the culture medium inside the shell.

2. The bioreactor vessel of claim 1, wherein, The vent pipe has multiple air outlets on its side wall.

3. The bioreactor vessel of claim 1, wherein, The venting tube can be a straight tube, a loop tube, or a serpentine tube.

4. The bioreactor vessel according to any of claims 1 to 3, characterized in that The air inlet (3) is connected to the air source, and a heat exchanger (8) is provided between the air source and the air inlet (3). The heat exchanger (8) is used to heat or cool the gas.

5. The biological culture container according to claim 4, characterized in that, The heat exchanger (8) directly or indirectly exchanges heat with the gas passing through it.

6. The bioreactor vessel of claim 5, wherein, The heat exchanger is equipped with heating / cooling plates or heating / cooling medium fluid pipelines.

7. The bioreactor vessel of any of claims 5-6, wherein, A gas sterilization device is connected to the gas source pipeline between the air inlet (3) and the gas inlet.

8. The bioreactor vessel of any one of claims 1 or 6, wherein, The housing (1) also includes a feed inlet (2) and a sampling port (5).

9. The biological culture container according to any one of claims 1 or 6, characterized in that, The housing (1) also includes a sensor interface (6).

10. The bioreactor vessel of any of claims 1-3, 6, wherein, The gas source provides air, oxygen, or carbon dioxide gas.

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