A multi-channel chemiluminescence detector

Abstract

The utility model provides a kind of multi-channel chemiluminescence detector, it is related to chemiluminescence detection technical field, the detector includes integral frame, reagent bin assembly and pipette assembly are equipped on integral frame;Reagent bin assembly includes multiple first reagent bin and second reagent bin, multiple first reagent bin can be mutually independent motion, to alternately execute detection task, and every first reagent bin is single channel and contains sample and reagent, and second reagent bin is multi-channel and contains sample and reagent;Pipette assembly includes multiple first pipette and second pipette, multiple first pipette can be mutually independent motion, to alternately execute detection task, every first pipette is single channel pipetting, and second pipette is multi-channel pipetting, and second pipette is matched with second reagent bin, reached when encountering emergency, without stopping, while in simultaneously detecting multiple samples, greatly improve the technical effect of detection efficiency.

Description

Technical Field

[0001] This utility model relates to the field of chemiluminescence detection technology, and in particular to a multi-channel chemiluminescence detector. Background Technology

[0002] Chemiluminescence immunoassay technology is crucial for clinical diagnosis, especially for high-precision detection of infectious disease markers and tumor markers. With the diversification of medical applications, equipment needs to simultaneously meet three conflicting requirements: High throughput and efficiency: Large hospitals conduct over a thousand tests per day, requiring shorter single-sample testing cycles; Emergency response capability: Samples from critically ill patients need to be inserted at any time, and routine testing procedures cannot be interrupted; Miniaturization and low cost: Primary healthcare institutions need lightweight equipment to reduce the burden of procurement and maintenance.

[0003] However, most single-dose chemiluminescence immunoassays on the market currently use multiple channels that move simultaneously. This layout is less efficient and requires waiting or shutdown in case of emergencies, which is very inconvenient.

[0004] Therefore, there is an urgent need to design a multi-channel chemiluminescence detector to solve the problems mentioned above. Utility Model Content

[0005] The purpose of this invention is to provide a multi-channel chemiluminescence detector to alleviate the technical problem of having to wait or stop the machine in the event of emergencies such as emergency situations in the existing technology.

[0006] This utility model provides a multi-channel chemiluminescence detector, which includes an overall frame, on which a reagent compartment assembly and a pipetting assembly are provided; The reagent compartment assembly includes multiple first reagent compartments and second reagent compartments. The multiple first reagent compartments can move independently to alternately perform detection tasks. Each first reagent compartment is a single-channel container for holding samples and reagents, while the second reagent compartments are multi-channel containers for holding samples and reagents. The pipetting assembly includes multiple first pipetting elements and second pipetting elements. The multiple first pipetting elements can move independently to perform detection tasks alternately. Each first pipetting element is a single-channel pipetting element and cooperates with its adjacent first reagent compartment element. The second pipetting element is a multi-channel pipetting element and cooperates with the second reagent compartment element.

[0007] In an optional implementation, The first reagent compartment includes a single-channel reagent compartment that can slide relative to the overall frame. The second reagent compartment includes a multi-channel reagent compartment that can slide relative to the overall frame along a first direction.

[0008] In an optional implementation, Both the first and second reagent compartments include a reagent drive group. The single-channel and multi-channel reagent compartments are driven by their respective reagent drive groups to slide relative to the overall frame along the first direction.

[0009] In an optional implementation, The reagent drive assembly includes a motor mounting plate, which is fixedly connected to the overall frame. A first lead screw motor is fixedly connected to the motor mounting plate. The output end of the first lead screw motor is connected to a first slider through a T-shaped lead screw nut. A first guide rail is provided on the overall frame. The first guide rail is slidably connected to the first slider. The first slider of the first reagent compartment is fixedly connected to the single-channel reagent compartment. The first slider of the second reagent compartment is fixedly connected to the multi-channel reagent compartment.

[0010] In an optional implementation, The first pipette includes a single-channel injection assembly, which can aspirate or dispense liquid into the corresponding first reagent compartment. The single-channel injection assembly can slide relative to the overall frame, allowing the single-channel injection assembly to be fitted with a TIP tip, break the membrane, and be fitted with a magnetic rod sleeve during sliding. The second pipette includes a multi-channel injection assembly, which can aspirate or dispense liquid into the corresponding second reagent compartment. The multi-channel injection assembly can slide relative to the overall frame along a second direction, allowing the multi-channel injection assembly to be fitted with a TIP tip, break the membrane, and be fitted with a magnetic rod sleeve during sliding.

[0011] In an optional implementation, Both the first and second pipetting units include a pipetting drive group. The single-channel injection group and the multi-channel injection group are driven by their respective corresponding pipetting drive groups to slide relative to the overall frame along the second direction. When the single-channel injection group or the multi-channel injection group slides downward along the second direction driven by its corresponding pipetting drive group, a TIP head can be fitted onto the single-channel injection group or the multi-channel injection group. Alternatively, the membrane can be broken by the single-channel injection group or the multi-channel injection group. A magnetic rod sleeve can also be fitted onto the single-channel injection group or the multi-channel injection group.

[0012] In an optional implementation, The pipetting drive assembly includes a second guide rail, on which a second lead screw motor is fixedly connected. The output end of the second lead screw motor is connected to a second slider via a T-shaped lead screw nut. The second slider is slidably connected to the second guide rail. The second slider of the first pipetting element is fixedly connected to a single-channel injection assembly, and the second slider of the second pipetting element is fixedly connected to a multi-channel injection assembly. In an optional implementation, The single-channel injection unit includes a third guide rail, which is fixedly connected to the second slider of the first pipette. A third lead screw motor is fixedly connected to the third guide rail, and the output end of the third lead screw motor is connected to the third slider via a T-shaped lead screw nut. The third slider is slidably connected to the third guide rail, and a single-channel piston rod is fixedly connected to the third slider. A single-channel piston cylinder is fixedly connected to the second slider of the first pipette, and the single-channel piston rod is slidably connected to the single-channel piston cylinder. A single-channel nozzle is connected to the single-channel piston cylinder. When the single-channel piston rod slides along the second direction in the single-channel piston cylinder, a negative or positive pressure is generated inside the single-channel piston cylinder to aspirate or expel liquid through the single-channel nozzle. When the single-channel nozzle needs to be separated from the TIP head, the single-channel piston rod slides downward along the second direction in the single-channel piston cylinder, pushing out the TIP head to separate the single-channel nozzle from the TIP head. When the magnetic bead needs to be transferred, the single-channel piston rod slides upward along the second direction in the single-channel piston cylinder, and the magnetic bead adsorbed on the magnetic rod falls off. In an optional implementation, The multi-channel injection unit includes a fourth guide rail, which is fixedly connected to the second slider of the second pipette. A fourth lead screw motor is fixedly connected to the fourth guide rail, and the output end of the fourth lead screw motor is connected to the fourth slider via a T-shaped lead screw nut. The fourth slider is slidably connected to the fourth guide rail, and a multi-channel piston rod is fixedly connected to the fourth slider. A multi-channel piston cylinder is fixedly connected to the second slider of the second pipette, and the multi-channel piston rod is slidably connected to the multi-channel piston cylinder. A multi-channel nozzle is connected to the multi-channel piston cylinder so that when the multi-channel piston rod slides in the multi-channel piston cylinder along the second direction, a negative or positive pressure is generated inside the multi-channel piston cylinder to aspirate or expel liquid through the multi-channel nozzle. When the multi-channel nozzle needs to be separated from the TIP head, the multi-channel piston rod slides downward in the multi-channel piston cylinder along the second direction, pushing the TIP head out to separate the multi-channel nozzle from the TIP head. When the magnetic bead needs to be transferred, the multi-channel piston rod slides upward in the multi-channel piston cylinder along the second direction, and the magnetic bead adsorbed on the magnetic rod falls off.

[0013] In an optional implementation, It also includes a PMT detection component, which moves to the detection position to perform detection and output the detection results after incubation is complete.

[0014] This invention employs multiple first reagent compartments and second reagent compartments, as well as multiple first pipetting pieces and second pipetting pieces. The multiple first reagent compartments and second reagent compartments are independent of each other and can alternately perform detection actions. The multiple first pipetting pieces and second pipetting pieces are also independent of each other and can alternately perform detection actions. Moreover, in case of emergency, there is no need to stop the machine. At the same time, multiple samples can be detected simultaneously, which greatly improves the detection efficiency. Attached Figure Description To more clearly illustrate the specific embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0015] Figure 1 This is a schematic diagram of the overall structure of the chemiluminescence detector of this utility model; Figure 2 This is a schematic diagram of the structure of the first reagent compartment of this utility model; Figure 3 This is a schematic diagram of the structure of the second reagent compartment of this utility model; Figure 4 This is a schematic diagram of the structure of the first pipetting component of this utility model; Figure 5 This is a schematic diagram of the structure of the second pipette of this utility model; Figure 6 This is a schematic diagram of the structure of the PMT detection component of this utility model; Figure 7 This is a schematic diagram of the support frame of this utility model; Figure 8 This is a schematic diagram of the overall framework of this utility model.

[0016] Icons: 1-Overall frame; 11-Support frame; 2-Reagent compartment assembly; 21-First reagent compartment component; 211-First lead screw motor; 212-Motor mounting plate; 213-First slider; 214-First guide rail; 215-Single-channel reagent compartment; 22-Second reagent compartment component; 225-Multi-channel reagent compartment; 3-Pipette assembly; 31-First pipette component; 311-Pipette drive assembly; 3111-Second lead screw motor; 3112-Second slider; 3113-Second guide rail; 312-Single-channel injection assembly; 3121-Third lead screw motor; 3122-Third slider; 3123-Third guide rail; 3124-Single-channel piston cylinder; 3125-Single-channel piston rod; 3126-Single-channel piston cylinder; 32-Second pipette; 322-Multi-channel injection assembly; 3221-Fourth lead screw motor; 3222-Fourth slider; 3223-Fourth guide rail; 3224-Multi-channel piston cylinder; 3225-Multi-channel piston rod; 3226-Multi-channel nozzle; 33-TIP head; 34-Magnetic rod sleeve; 4-PMT detection assembly; 41-PMT component; 411-PMT; 412-Servo motor; 413-PMT mounting plate; 414-PMT adapter plate; 42-Horizontal motion component; 421-Stepper motor; 422-Synchronous pulley; 423-Synchronous belt; 43-Vertical motion component; 431-Vertical lead screw motor; 432-Fifth slider; 433-Fifth guide rail. Detailed Implementation

[0017] The technical solution of this utility model will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0018] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0019] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0020] The specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit the scope of this utility model.

[0021] Currently, most single-dose chemiluminescence immunoassays on the market use multiple channels that move simultaneously. This layout is less efficient, and in case of emergencies such as urgent situations, it requires waiting or shutdown, which is very inconvenient.

[0022] Therefore, this multi-channel chemiluminescence detector includes an overall frame 1, on which a reagent compartment assembly 2 and a pipetting assembly 3 are provided, and also includes a support frame 11, which is fixedly connected to the overall frame 1. The reagent compartment assembly 2 includes multiple first reagent compartments 21 and second reagent compartments 22. The multiple first reagent compartments 21 can move independently to alternately perform detection tasks. Each first reagent compartment 21 is a single-channel container for holding samples and reagents. In case of an emergency, the first reagent compartments 21 can be used for detection without stopping the other first reagent compartments 21 and second reagent compartments 22, while the remaining first reagent compartments 21 continue to detect. The second reagent compartments 22 are multi-channel containers for holding samples and reagents. The second reagent compartments 22 can simultaneously detect multiple samples. When the number of samples to be detected is small, the first reagent compartments 21 can be used for detection. When the number of samples to be detected meets the multi-channel capacity of the second reagent compartments 22, the second reagent compartments 22 can be used for detection. Preferably, the number of first reagent compartments 21 is three, so that in case of an emergency, there is no need for other first reagent compartments 21 to be tested and the machine stopped. In other embodiments of this utility model, the number of first reagent compartments 21 can also be other, as long as the number of first reagent compartments 21 is not less than two.

[0023] Preferably, the second reagent compartment 22 has five channels to accommodate samples and reagents, so as to accommodate five samples and reagents at the same time and to detect the five samples at the same time. In other embodiments of this utility model, the second reagent compartment 22 can also accommodate samples and reagents with other numbers of channels, which can be set according to actual needs. The pipetting assembly 3 includes multiple first pipettes 31 and second pipettes 32. The multiple first pipettes 31 can move independently to alternately perform detection tasks. Each first pipette 31 is a single-channel pipette. In case of emergency, the first pipette 31 can be used to perform detection without stopping the machine, without needing to stop the detection of other first pipettes 31. The remaining first pipettes 31 and second pipettes 32 can continue to perform detection. The first pipette 31 is matched with its adjacent first reagent compartment 21, that is, one first pipette 31 corresponds to one first reagent compartment 21. The second pipette 32 is a multi-channel pipette. The second pipette 32 can be used to detect multiple samples at the same time. When the number of samples to be detected is small, the second pipette 32 can be used for detection. When the number of samples to be detected meets the multi-channel capacity of the second pipette 32, the second pipette 32 can be used for detection. The second pipette 32 is matched with the second reagent compartment 22.

[0024] Preferably, the number of first pipettes 31 is three, so that in case of an emergency, there is no need to stop the machine without checking other first pipettes 31. In other embodiments of this utility model, the number of first reagent compartments 21 can also be other, as long as the number of first pipettes 31 is the same as the number of first reagent compartments 21.

[0025] Preferably, the second pipette 32 is a five-channel pipette, which can simultaneously detect five samples. In other embodiments of this utility model, the second reagent compartment 22 can also be a pipette with other channel numbers, as long as the number of channels of the second pipette 32 is the same as the number of channels of the second reagent compartment 22.

[0026] This invention employs multiple first reagent compartments 21 and second reagent compartments 22, as well as multiple first pipettes 31 and second pipettes 32. The multiple first reagent compartments 21 and second reagent compartments 22 are independent of each other and can alternately perform detection actions. The multiple first pipettes 31 and second pipettes 32 are also independent of each other and can alternately perform detection actions. Furthermore, in case of an emergency, there is no need to stop the machine. At the same time, multiple samples can be detected simultaneously, which greatly improves the detection efficiency. The first reagent compartment 21 includes a single-channel reagent compartment 215, which can slide relative to the overall frame 1. The second reagent compartment 22 includes a multi-channel reagent compartment 225, which can slide relative to the overall frame 1 along a first direction A.

[0027] The multi-channel reagent compartment 225 is composed of multiple single-channel reagent compartments 215, and the single-channel reagent compartment 215 includes a sample well, a reagent well, and a reaction detection well. The sample wells are used to store biological samples to be tested; The reagent wells are for pre-loading chemiluminescent reagents; The reaction detection well is the final location where the sample and reagent are mixed and incubated.

[0028] Both the first reagent compartment 21 and the second reagent compartment 22 include a reagent drive group. The single-channel reagent compartment 215 and the multi-channel reagent compartment 225 are driven by their respective reagent drive groups to slide relative to the overall frame 1 along the first direction A. Both the single-channel reagent compartment 215 and the multi-channel reagent compartment 225 can slide relative to the overall frame 1 along the first direction A to ensure that the first pipette 31 and the second pipette 32 are accurately inserted into the required sample well, reagent well or reaction detection well in different steps.

[0029] Specifically, the first direction A is the Y-axis of this utility model.

[0030] The reagent drive assembly includes a motor mounting plate 212, which is fixedly connected to the overall frame. A first lead screw motor 211 is fixedly connected to the motor mounting plate 212. The output end of the first lead screw motor 211 is connected to a first slider 213 via a T-shaped lead screw nut. A first guide rail 214 is provided on the overall frame, and the first guide rail 214 is slidably connected to the first slider 213. The first slider 213 of the first reagent compartment 21 is fixedly connected to a single-channel reagent compartment 215, and the first slider 213 of the second reagent compartment 22 is fixedly connected to a multi-channel reagent compartment 225. Specifically, after the first lead screw motor 211 is started, the output end of the first lead screw motor 211 rotates, and then the rotational motion is converted into linear motion via the T-shaped lead screw nut, causing the first slider 213 to slide along the first guide rail 214. Thus, the first slider 213 of the first reagent compartment 21 drives the single-channel reagent compartment 215 to slide, and the first slider 213 of the second reagent compartment 22 drives the multi-channel reagent compartment 225 to slide.

[0031] The first pipette 31 includes a single-channel injection assembly 312, which can aspirate or dispense liquid into the corresponding first reagent compartment 21. The single-channel injection assembly 312 can slide relative to the overall frame 1, allowing the single-channel injection assembly 312 to be fitted with a TIP tip 33, break the membrane, and be fitted with a magnetic rod sleeve 34 during sliding. The second pipette 32 includes a multi-channel injection assembly 322, which can aspirate or dispense liquid into the corresponding second reagent compartment. The multi-channel injection assembly 322 can slide relative to the overall frame 1 along the second direction B, allowing the multi-channel injection assembly 322 to be fitted with a TIP tip 33, break the membrane, and be fitted with a magnetic rod sleeve 34 during sliding.

[0032] TIP tip 33 is a disposable plastic consumable that comes into direct contact with liquid samples and reagents, functioning similarly to the tip of a laboratory pipette.

[0033] The first pipetting component 31 and the second pipetting component 32 of this utility model combine the injection pump core and the magnetic rod into one part, thereby integrating functions such as magnetic bead transfer, pipetting, TIP head 33 membrane breaking, and TIP head 33 unloading. Compared with the existing separate modules, the number of parts is reduced by 50%, and the function switching time is reduced, saving detection time.

[0034] Both the first pipetting unit 31 and the second pipetting unit 32 include a pipetting drive group 311. The single-channel injection group 312 and the multi-channel injection group 322 are driven by their respective pipetting drive groups 311 to slide relative to the overall frame 1 along the second direction B. When the single-channel injection group 312 or the multi-channel injection group 322 slides downward along the second direction B driven by its corresponding pipetting drive group 311, a TIP head 33 can be fitted onto the single-channel injection group 312 or the multi-channel injection group 322, the membrane can be broken by the single-channel injection group 312 or the multi-channel injection group 322, and a magnetic rod sleeve 34 can be fitted onto the single-channel injection group 312 or the multi-channel injection group 322.

[0035] The pipetting drive assembly 311 includes a second guide rail 3113, on which a second lead screw motor 3111 is fixedly connected. The output end of the second lead screw motor 3111 is connected to a second slider 3112 via a T-shaped lead screw nut. The second slider 3112 is slidably connected to the second guide rail 3113. The second slider 3112 of the first pipetting element 31 is fixedly connected to the single-channel injection assembly 312, and the second slider 3112 of the second pipetting element 32 is fixedly connected to the multi-channel injection assembly 322. Specifically, after the second lead screw motor 3111 is started, its output end rotates, and then the rotational motion is converted into linear motion via the T-shaped lead screw nut, causing the second slider 3112 to slide along the second guide rail 3113. Thus, the second slider 3112 of the first pipetting element 31 drives the single-channel injection assembly 312 to slide, and the second slider 3112 of the second reagent compartment 22 drives the multi-channel injection assembly 322 to slide. The single-channel injection unit 312 includes a third guide rail 3123, which is fixedly connected to the second slider 3112 of the first pipette 31. A third lead screw motor 3121 is fixedly connected to the third guide rail 3123. The output end of the third lead screw motor 3121 is connected to a third slider 3122 via a T-shaped lead screw nut. The third slider 3122 is slidably connected to the third guide rail 3123. A single-channel piston rod 3125 is fixedly connected to the third slider 3122. A single-channel piston cylinder 3124 is fixedly connected to the second slider 3112 of the first pipette 31. The single-channel piston rod 3125 is slidably connected to the single-channel piston cylinder 3124. A single-channel nozzle 3126 is connected to the single-channel piston cylinder 3124 so that a negative pressure is generated inside the single-channel piston cylinder 3124 when the single-channel piston rod 3125 slides in the single-channel piston cylinder 3124 along the second direction B. Alternatively, positive pressure can be applied to suction or discharge liquid through the single-channel suction nozzle 3126. When the single-channel suction nozzle 3126 needs to be separated from the TIP head 33, the single-channel piston rod 3125 slides downward along the second direction B in the single-channel piston cylinder 3124, pushing the TIP head 33 out to separate the single-channel suction nozzle 3126 from the TIP head 33. When the magnetic bead needs to be transferred, the single-channel piston rod 3125 slides upward along the second direction B in the single-channel piston cylinder 3124, and the magnetic bead adsorbed on the magnetic rod sleeve 34 falls off. Specifically, after the third lead screw motor 3121 is started, the output end of the third lead screw motor 3121 rotates, and then the rotational motion is converted into linear motion through the T-shaped lead screw nut, so that the third slider 3122 slides along the third guide rail 3123, thereby the third slider 3122 drives the single-channel piston rod 3125 to slide along the single-channel piston cylinder 3124. Specifically, the second direction B is the Z-axis of this utility model.

[0036] Specifically, the single-channel piston rod 3125 is provided with a magnetic segment, which is magnetic. The magnetic segment can be moved up and down along the second direction B by the single-channel piston rod 3125 to control whether the magnetic rod sleeve 34 is magnetic.

[0037] The working process of the first pipetting element 31: Step 1: After the first pipette 31 moves to the position of the TIP head 33, the second lead screw motor 3111 drives the single-channel injection assembly 312 to descend along the second direction B until the single-channel nozzle 3126 is fitted into the TIP head 33. Step 2: The first pipette 31 moves to the sample position. The second lead screw motor 3111 drives the single-channel injection assembly 312 to descend along the second direction B to a specific height. The sealing film on the sample position is punctured through the TIP head 33. The third lead screw motor 3121 drives the single-channel piston rod 3125 to move upward along the second direction B to form a negative pressure, thereby drawing the sample from the single-channel nozzle 3126 into the single-channel piston cylinder 3124. Step 3: The first pipette 31 moves to a specific reagent position. The second lead screw motor 3111 drives the single-channel injection assembly 312 to descend to a specific height along the second direction B. Then, the third lead screw motor 3121 drives the single-channel piston rod 3125 to move downward along the second direction B to form positive pressure, thereby dispensing the sample into the reagent position. Step 4: The first pipette 31 moves to the position where the TIP head 33 is located. The second lead screw motor 3111 drives the single-channel injection assembly 312 to descend along the second direction B to a specific height. Then, the third lead screw motor 3121 drives the single-channel piston rod 3125 to move downward along the second direction B. The single-channel piston rod 3125 pushes out the TIP head 33, separating the TIP head 33 from the single-channel suction nozzle 3126, thereby unloading the TIP head 33. Step 5: The first pipette 31 moves to the position of the magnetic rod sleeve 34, and the second lead screw motor 3111 drives the single-channel injection group 312 to descend along the second direction B until the single-channel nozzle 3126 is fitted into the magnetic rod sleeve 34. Step 6: The first pipette 31 moves to the reagent position where the sample has been dispensed. The second lead screw motor 3111 drives the single-channel injection assembly 312 to move up and down along the second direction B, causing the magnetic rod sleeve 34 to vibrate the reagent and sample rapidly, thereby allowing the sample and liquid to fully mix and react. After the full mixing and reaction, the third lead screw motor 3121 drives the single-channel piston rod 3125 to move down along the second direction B to a specific position, so that the magnetic beads in the sample reagent are adsorbed onto the surface of the magnetic rod sleeve 34 through the magnetic segment of the single-channel piston rod 3125.

[0038] Step 7: The first pipette 31 moves to the next reagent position. The second lead screw motor 3111 drives the single-channel injection assembly 312 to descend along the second direction B to a specific height. When the third lead screw motor 3121 drives the single-channel piston rod 3125 to move upward along the second direction B to a specific position, the magnetic segment of the single-channel piston rod 3125 moves away from the magnetic rod sleeve 34 and loses its magnetism from the magnetic rod sleeve 34. The magnetic bead on the magnetic rod sleeve 34 falls into the reagent position, thereby realizing the function of magnetic bead transfer.

[0039] The multi-channel injection unit 322 includes a fourth guide rail 3223, which is fixedly connected to the second slider 3112 of the second pipette 32. A fourth lead screw motor 3221 is fixedly connected to the fourth guide rail 3223. The output end of the fourth lead screw motor 3221 is connected to the fourth slider 3222 via a T-shaped lead screw nut. The fourth slider 3222 is slidably connected to the fourth guide rail 3223. A multi-channel piston rod 3225 is fixedly connected to the fourth slider 3222. A multi-channel piston cylinder 3224 is fixedly connected to the second slider 3112 of the second pipette 32. The multi-channel piston rod 3225 is slidably connected to the multi-channel piston cylinder 3224. A multi-channel nozzle 3226 is connected to the multi-channel piston cylinder 3224 to generate negative pressure inside the multi-channel piston cylinder 3224 when the multi-channel piston rod 3225 slides in the multi-channel piston cylinder 3224 along the second direction B. Positive pressure is applied to suction or discharge liquid through the multi-channel suction nozzle 3226. When the multi-channel suction nozzle 3226 needs to be separated from the TIP head 33, the multi-channel piston rod 3225 slides downward along the second direction B in the multi-channel piston cylinder 3224, pushing the TIP head 33 out to separate the multi-channel suction nozzle 3226 from the TIP head 33. When the magnetic bead needs to be transferred, the multi-channel piston rod 3225 slides upward along the second direction B in the multi-channel piston cylinder 3224, and the magnetic bead adsorbed on the magnetic rod sleeve 34 falls off. Specifically, after the fourth lead screw motor 3221 is started, the output end of the fourth lead screw motor 3221 rotates, and then the rotational motion is converted into linear motion through the T-shaped lead screw nut, so that the fourth slider 3222 slides along the fourth guide rail 3223, thereby the fourth slider 3222 drives the multi-channel piston rod 3225 to slide along the multi-channel piston cylinder 3224. Specifically, the multi-channel piston rod 3225 is provided with a magnetic segment, which is magnetic. The magnetic segment can be moved up and down along the second direction B by the multi-channel piston rod 3225 to control whether the magnetic rod sleeve 34 is magnetic.

[0040] The working process of the second pipette 32: Step 1: After the second pipette 32 moves to the position of the TIP head 33, the second lead screw motor 3111 drives the multi-channel injection assembly 322 to descend along the second direction B until the multi-channel nozzle 3226 is fitted into the TIP head 33. Step 2: The second pipette 32 moves to the sample position. The second lead screw motor 3111 drives the multi-channel injection assembly 322 to descend along the second direction B to a specific height. The sealing film on the sample position is punctured through the TIP head 33. The fourth lead screw motor 3221 drives the multi-channel piston rod 3225 to move upward along the second direction B to form a negative pressure, thereby drawing the sample from the multi-channel nozzle 3226 into the multi-channel piston cylinder 3224. Step 3: The second pipette 32 moves to the specific reagent position. The second lead screw motor 3111 drives the multi-channel injection group 322 to descend to a specific height along the second direction B. Then, the fourth lead screw motor 3221 drives the multi-channel piston rod 3225 to move downward along the second direction B to form positive pressure, thereby dispensing the sample into the reagent position. Step 4: The second pipette 32 moves to the position where the TIP head 33 is located. The second lead screw motor 3111 drives the multi-channel injection assembly 322 to descend along the second direction B to a specific height. Then, the fourth lead screw motor 3221 drives the multi-channel piston rod 3225 to move downward along the second direction B. The multi-channel piston rod 3225 pushes out the TIP head 33, separating the TIP head 33 from the multi-channel nozzle 3226, thereby unloading the TIP head 33. Step 5: The second pipette 32 moves to the position of the magnetic rod sleeve 34, and the second lead screw motor 3111 drives the multi-channel injection group 322 to descend along the second direction B until the multi-channel nozzle 3226 is fitted into the magnetic rod sleeve 34. Step 6: The second pipette 32 moves to the reagent position where the sample has been dispensed. The second lead screw motor 3111 drives the multi-channel injection assembly 322 to move up and down along the second direction B, causing the magnetic rod sleeve 34 to vibrate the reagent and sample rapidly, thereby allowing the sample and liquid to fully mix and react. After the full mixing and reaction, the fourth lead screw motor 3221 drives the multi-channel piston rod 3225 to move down along the second direction B to a specific position, so that the magnetic beads in the sample reagent are adsorbed onto the surface of the magnetic rod sleeve 34 through the magnetic segment of the multi-channel piston rod 3225.

[0041] Step 7: The second pipette 32 moves to the next reagent position. The second lead screw motor 3111 drives the multi-channel injection assembly 322 to descend along the second direction B to a specific height. When the fourth lead screw motor 3221 drives the multi-channel piston rod 3225 to move upward along the second direction B to a specific position, the magnetic segment of the multi-channel piston rod 3225 moves away from the magnetic rod sleeve 34 and loses its magnetism from the magnetic rod sleeve 34. The magnetic bead on the magnetic rod sleeve 34 falls into the reagent position, thereby realizing the function of magnetic bead transfer.

[0042] It also includes a PMT detection component 4, which moves to the detection position to perform detection and output the detection result after incubation is completed.

[0043] The PMT detection component 4 includes a PMT component 41, a horizontal motion component 42 that drives the PMT component 41 to move horizontally, a vertical motion component 43 that drives the PMT component 41 to move vertically, and a rotating motion component of the light-avoiding shutter.

[0044] The horizontal motion component 42 includes a stepper motor 421. The output end of the stepper motor 421 drives a synchronous pulley 422. The synchronous belt 423 engaged by the synchronous pulley 422 is connected to the PMT component 41 and the vertical motion component 43 mounted on the guide rail slider, thereby driving the PMT component 41 and the vertical motion component 43 to perform horizontal movement.

[0045] The vertical motion component 43 includes a vertical lead screw motor 431. The output end of the vertical lead screw motor 431 is connected to the fifth slider 432 through a T-shaped lead screw nut. The fifth slider 432 is slidably connected to the fifth guide rail 433. A PMT adapter plate 414 is connected to the fifth slider 432. The PMT adapter plate 414 is fixedly connected to the PMT component 41, thereby realizing the vertical up and down movement of the PMT component 41.

[0046] PMT component 41 includes PMT adapter plate 414, PMT mounting plate 413 is fixedly connected to PMT adapter plate 414, PMT 411 and servo motor 412 are mounted on PMT mounting plate 413, PMT 412 is connected to servo motor 412 and PMT light shield is controlled by servo motor 412 to rotate, thereby realizing the function of opening when testing and closing the light shield when not testing.

[0047] Another innovation of PMT component 41 is its excellent sealing performance, which makes it difficult for external light to penetrate and affect the test results of PMT411. Sealing rings are provided between PMT411 and PMT mounting plate 413, between servo motor 412 and PMT mounting plate 413, and between PMT mounting plate 413 and PMT light shield to increase sealing performance.

[0048] PMT is an optical sensor that detects chemiluminescence signals, enabling quantitative analysis by converting weak photons into electrical signals.

[0049] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this utility model.

Claims

1. A multi-channel chemiluminescence detector, characterized in that, It includes an overall frame (1), on which a reagent compartment assembly (2) and a pipetting assembly (3) are provided; The reagent compartment assembly (2) includes multiple first reagent compartments (21) and second reagent compartments (22). The multiple first reagent compartments (21) can move independently to alternately perform detection tasks. Each first reagent compartment (21) is a single-channel container for holding samples and reagents, and the second reagent compartment (22) is a multi-channel container for holding samples and reagents. The pipetting assembly (3) includes multiple first pipettes (31) and second pipettes (32). The multiple first pipettes (31) can move independently to perform detection tasks alternately. Each first pipette (31) is a single-channel pipette and cooperates with its adjacent first reagent compartment (21). The second pipette (32) is a multi-channel pipette and cooperates with the second reagent compartment (22).

2. The multi-channel chemiluminescence detector according to claim 1, characterized in that, The first reagent compartment (21) includes a single-channel reagent compartment (215), which is slidable relative to the overall frame (1). The second reagent compartment (22) includes a multi-channel reagent compartment (225), which is slidable relative to the overall frame (1) along a first direction.

3. The multi-channel chemiluminescence detector according to claim 2, characterized in that, The first reagent compartment (21) and the second reagent compartment (22) both include a reagent drive group. The single-channel reagent compartment (215) and the multi-channel reagent compartment (225) are driven by their respective reagent drive groups to slide relative to the overall frame (1) along the first direction.

4. The multi-channel chemiluminescence detector according to claim 3, wherein, The reagent drive assembly includes a motor mounting plate (212), which is fixedly connected to the overall frame (1). A first lead screw motor (211) is fixedly connected to the motor mounting plate (212). The output end of the first lead screw motor (211) is connected to a first slider (213) through a T-shaped lead screw nut. A first guide rail (214) is provided on the overall frame (1). The first guide rail (214) is slidably connected to the first slider (213). The first slider (213) of the first reagent compartment (21) is fixedly connected to the single-channel reagent compartment (215). The first slider (213) of the second reagent compartment (22) is fixedly connected to the multi-channel reagent compartment (225).

5. The multi-channel chemiluminescence detector according to claim 1, wherein, The first pipette (31) includes a single-channel injection assembly (312), which can aspirate or dispense liquid from the corresponding first reagent compartment (21). The single-channel injection assembly (312) can slide relative to the overall frame (1), so that the single-channel injection assembly (312) can be fitted with a TIP tip (33), break the membrane, and fit a magnetic rod sleeve (34) when sliding. The second pipette (32) includes a multi-channel injection assembly (322), which can aspirate or dispense liquid from the corresponding second reagent compartment (22). The multi-channel injection assembly (322) can slide relative to the overall frame (1) along a second direction, so that the multi-channel injection assembly (322) can be fitted with a TIP tip (33), break the membrane, and fit a magnetic rod sleeve (34) when sliding.

6. The multi-channel chemiluminescence detector according to claim 5, wherein, The first pipette (31) and the second pipette (32) both include a pipetting drive group (311). The single-channel injection group (312) and the multi-channel injection group (322) are driven by their respective pipetting drive groups (311) to slide relative to the overall frame (1) along the second direction. When the single-channel injection group (312) or the multi-channel injection group (322) slides downward along the second direction driven by the corresponding pipetting drive group (311), a TIP head (33) can be put on the single-channel injection group (312) or the multi-channel injection group (322). The membrane can also be broken by the single-channel injection group (312) or the multi-channel injection group (322). A magnetic rod sleeve (34) can also be put on the single-channel injection group (312) or the multi-channel injection group (322).

7. The multi-channel chemiluminescence detector according to claim 6, characterized in that, The pipetting drive assembly (311) includes a second guide rail (3113), on which a second lead screw motor (3111) is fixedly connected. The output end of the second lead screw motor (3111) is connected to a second slider (3112) through a T-shaped lead screw nut. The second slider (3112) is slidably connected to the second guide rail (3113). The second slider (3112) of the first pipetting element (31) is fixedly connected to the single-channel injection assembly (312), and the second slider (3112) of the second pipetting element (32) is fixedly connected to the multi-channel injection assembly (322).

8. The multi-channel chemiluminescence detector according to claim 7, wherein, The single-channel injection assembly (312) includes a third guide rail (3123), which is fixedly connected to the second slider (3112) of the first pipette (31). A third lead screw motor (3121) is fixedly connected to the third guide rail (3123). The output end of the third lead screw motor (3121) is connected to a third slider (3122) via a T-shaped lead screw nut. The third slider (3122) is slidably connected to the third guide rail (3123). A single-channel piston rod (3125) is fixedly connected to the third slider (3122). A single-channel piston cylinder (3124) is fixedly connected to the second slider (3112) of the first pipette (31). The single-channel piston rod (3125) is slidably connected to the single-channel piston cylinder (3124). A single-channel suction nozzle (3126) is connected to the single-channel piston rod (3125) so that when the single-channel piston rod (3125) slides in the single-channel piston cylinder (3124) along the second direction, a negative or positive pressure is generated in the single-channel piston cylinder (3124) so ​​that liquid can be sucked or expelled through the single-channel suction nozzle (3126). When the single-channel suction nozzle (3126) needs to be separated from the TIP head (33), the single-channel piston rod (3125) slides downward in the single-channel piston cylinder (3124) along the second direction and pushes out the TIP head (33) through the single-channel piston rod (3125) so as to separate the single-channel suction nozzle (3126) from the TIP head (33). When the magnetic bead needs to be transferred, the single-channel piston rod (3125) slides upward in the single-channel piston cylinder (3124) along the second direction and the magnetic bead adsorbed on the magnetic rod sleeve (34) falls off.

9. The multi-channel chemiluminescence detector according to claim 7, characterized in that, The multi-channel injection assembly (322) includes a fourth guide rail (3223), which is fixedly connected to the second slider (3112) of the second pipette (32). A fourth lead screw motor (3221) is fixedly connected to the fourth guide rail (3223). The output end of the fourth lead screw motor (3221) is connected to the fourth slider (3222) via a T-shaped lead screw nut. The fourth slider (3222) is slidably connected to the fourth guide rail (3223). A multi-channel piston rod (3225) is fixedly connected to the fourth slider (3222). A multi-channel piston cylinder (3224) is fixedly connected to the second slider (3112) of the second pipette (32). The multi-channel piston rod (3225) is slidably connected to the multi-channel piston cylinder (3224). A multi-channel suction nozzle (3226) is connected to 224. When the multi-channel piston rod (3225) slides in the multi-channel piston cylinder (3224) along the second direction, a negative or positive pressure is generated in the multi-channel piston cylinder (3224) to suck or expel liquid through the multi-channel suction nozzle (3226). When the multi-channel suction nozzle (3226) needs to be separated from the TIP head (33), the multi-channel piston rod (3225) slides downward in the multi-channel piston cylinder (3224) along the second direction and pushes out the TIP head (33) through the multi-channel piston rod (3225) to separate the multi-channel suction nozzle (3226) from the TIP head (33). When the magnetic bead needs to be transferred, the multi-channel piston rod (3225) slides upward in the multi-channel piston cylinder (3224) along the second direction and the magnetic bead adsorbed on the magnetic rod sleeve (34) falls off.

10. The multi-channel chemiluminescence detector according to claim 1, characterized in that, It also includes a PMT detection component (4). After incubation is complete, the PMT detection component (4) moves to the detection position to perform detection and output the detection result.

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