Chromatographic purification apparatus for use in chemical production processes

Abstract

The utility model discloses a chromatography purification equipment in chemical production process. Including buffer bottle, buffer bottle is connected chromatography column through first delivery pipeline, the import of chromatography column is connected with the original liquid bottle through second delivery pipeline, is provided with pressurizing mechanism on chromatography column, and the controlled end of pressurizing mechanism is connected with PLC controller, and the chromatography column is equipped with the temperature adjusting frame, and is provided with the package column cylinder on the temperature adjusting frame, and the liquid outlet of chromatography column is connected with the receiving bottle through third delivery pipeline, and third delivery pipeline is connected with fourth delivery pipeline, and fourth delivery pipeline is connected with waste liquid bottle, and pump body and solenoid valve are all set up on first delivery pipeline, second delivery pipeline, and the controlled end of pump body and solenoden valve is connected with PLC controller, and PLC controller is connected with audible -visual annunciator, the utility model has the advantages of high degree of automation, simple structure, high chromatography efficiency, can realize the accurate temperature control to chromatography purification process.

Description

Technical Field

[0001] This utility model relates to the field of analytical purification equipment technology, and more specifically to a chromatography purification device for chemical production processes. Background Technology

[0002] Chromatography is short for "chromatographic analysis." It is a method of separating and determining multi-component mixtures by utilizing the differences in the physical properties of their components. There are two types: adsorption chromatography and partition chromatography. It is generally used for the analysis of organic compounds, metal ions, amino acids, etc. Chromatography utilizes the different partition ratios of substances between the stationary phase and the mobile phase to achieve separation. Chromatography has extremely high resolution for the separation and analysis of complex organic mixtures of biological macromolecules such as proteins and nucleic acids.

[0003] In the process of chromatographic purification, some solutions need to be kept at low or high temperatures. However, existing chromatographic purification equipment is characterized by its large size and complicated structure, and cannot effectively cool or heat the solution. Therefore, there is a need for a chromatographic purification device for chemical production processes. Utility Model Content

[0004] The technical problem to be solved by this utility model is to provide a chromatography purification device for chemical production processes, so as to solve the problems in the background art.

[0005] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is as follows.

[0006] A chromatography purification device for chemical production processes includes several buffer bottles connected to a first delivery pipeline, the other end of which is connected to a vertically mounted chromatography column with a pressure chamber. The inlet of the chromatography column is connected to a second delivery pipeline, the other end of which is connected to a stock solution containing the solution to be purified by chromatography. The chromatography column is equipped with a pressurization mechanism to accelerate the chromatography process, and the controlled end of the pressurization mechanism is connected to a PLC controller. A temperature control rack with a hollow structure is provided outside the chromatography column for adjusting the column temperature. The temperature control rack is equipped with several vertically arranged column tubes, and the temperature control rack is filled with liquid for heat absorption or release; the outlet of the chromatography column is connected to a third delivery pipeline, and the other end of the third delivery pipeline is connected to a receiving bottle for receiving samples; a fourth delivery pipeline is connected to the third delivery pipeline, and the other end of the fourth delivery pipeline is connected to a waste liquid bottle for collecting waste liquid; pumps and solenoid valves are installed on the first and second delivery pipelines, and the controlled ends of the pumps and solenoid valves are connected to a PLC controller, and the PLC controller is connected to an audible and visual alarm.

[0007] To further optimize the technical solution, the first, second, third, and fourth conveying pipelines are made of stainless steel or polytetrafluoroethylene.

[0008] To further optimize the technical solution, the temperature control rack is made of one of the following materials: plastic, resin, or metal.

[0009] To further optimize the technical solution, the temperature control frame is provided with an inlet at the top and an outlet at the bottom.

[0010] To further optimize the technical solution, the liquid inside the temperature control frame is water, and a temperature sensor is installed inside the temperature control frame. The output terminal of the temperature sensor is connected to a PLC controller.

[0011] To further optimize the technical solution, the pressurization mechanism includes a nitrogen cylinder connected to a pressurization pipeline; the other end of the pressurization pipeline is connected to the pressurization chamber of the chromatography column, and a pressurizer is installed on the pressurization pipeline.

[0012] Due to the adoption of the above technical solutions, the technological progress achieved by this utility model is as follows.

[0013] This invention provides a chromatography purification device for chemical production processes. By connecting the chromatography column to a pressurizing mechanism, the chromatography speed is effectively accelerated. Furthermore, by installing a temperature control rack outside the chromatography column, precise temperature control of the chromatography process is achieved, which is beneficial for improving product quality. This invention has the advantages of high automation, simple structure, high chromatography efficiency, and precise temperature control of the chromatography purification process. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2 This is a schematic diagram of the temperature control frame in this utility model; Figure 3 This is a schematic diagram of another structure of the temperature regulating rack in this utility model.

[0015] The components are: 1. Buffer solution bottle, 2. Stock solution bottle, 3. Chromatography column, 4. Nitrogen cylinder, 5. Pressurizer, 6. Receiving bottle, 7. Waste liquid bottle, 8. Temperature control rack, 9. Column casing, 10. Inlet, 11. Outlet, 12. Temperature sensor, 13. First delivery line, 14. Second delivery line, 15. Third delivery line, 16. Fourth delivery line. Detailed Implementation

[0016] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments.

[0017] A chromatography purification device for chemical production processes, used in conjunction with detection equipment, combined with... Figures 1 to 3As shown, it includes a buffer bottle 1, a stock solution bottle 2, a chromatography column 3, a nitrogen bottle 4, a pressurizer 5, a receiving bottle 6, a waste liquid bottle 7, a temperature control rack 8, a column casing 9, an inlet 10, an outlet 11, a temperature sensor 12, a first delivery line 13, a second delivery line 14, a third delivery line 15, and a fourth delivery line 16.

[0018] Buffer bottle 1 contains buffer solution, which is used for cleaning, equilibration, elution and sterilization of chromatography column 3.

[0019] The buffer bottle 1 is connected to the first delivery line 13, and the other end of the first delivery line 13 is connected to the corresponding chromatography column 3. The chromatography column 3 is vertically arranged and has a pressure chamber at the top. The chromatography column 3 is filled with nanoscale packing material, which has a larger specific surface area and a more uniform pore size distribution, increasing the interaction sites between the packing material and sample molecules, thus improving the separation efficiency by more than 30% compared with the traditional chromatography column 3.

[0020] The inlet of the chromatography column 3 is connected to a second delivery line 14, and the other end of the second delivery line 14 is connected to a stock solution bottle 2, which contains the solution to be purified by chromatography.

[0021] The chromatography column 3 is equipped with a pressurization mechanism to accelerate the chromatography process. The controlled end of the pressurization mechanism is connected to a PLC controller. The pressurization mechanism includes a nitrogen cylinder 4, which is connected to a pressurization pipeline. The other end of the pressurization pipeline is connected to the pressurization chamber of the chromatography column 3, and a pressurizer is installed on the pressurization pipeline. The pressurizer is a pressurizer with a pressure regulating valve, and the chromatography column 3 is equipped with a pressure relief valve.

[0022] A temperature control frame 8 with a hollow structure is provided outside the chromatography column 3 for adjusting the temperature of the chromatography column 3, and the temperature control frame 8 is filled with a liquid for heat absorption or release. The temperature control frame 8 is installed on the outside of the chromatography column 3 via a support frame, connecting rod, etc. The temperature control frame 8 is made of one of the following materials: plastic, resin, or metal. A liquid inlet 10 is provided at the top of the temperature control frame 8, and a liquid outlet 11 is provided at the bottom. The liquid inside the temperature control frame 8 is water, and a temperature sensor 12 is installed inside the temperature control frame 8. The output terminal of the temperature sensor 12 is connected to a PLC controller. Several vertically arranged column sleeves 9 are inserted through the temperature control frame 8. The sidewalls of the column sleeves 9 are in contact with the sidewalls of the chromatography column 3 to conduct heat. The temperature control frame 8 can maintain the low temperature at room temperature for a relatively long time (15 minutes to 2 hours) after reaching the low temperature state. Depending on the required temperature, the liquid in the temperature control rack 8 can be replaced. Similarly, after the liquid in the temperature control rack 8 has been heated, a low-temperature solution can be continuously fed into the temperature control rack 8 by connecting it to the liquid inlet 10. Likewise, if the chromatography process requires continuous heating, a liquid of appropriate temperature can be directly filled into the temperature control rack 8, or a warm liquid can be continuously circulated into the temperature control rack 8.

[0023] The temperature control rack 8 can be paired with a single chromatography column, or multiple column packing tubes can be set on one temperature control rack to simultaneously control the temperature of multiple chromatography columns.

[0024] The outlet of the chromatography column 3 is connected to the third delivery line 15, and the other end of the third delivery line 15 is connected to the receiving bottle 6 for receiving samples. The receiving bottle 6 is connected to a detection device, which can monitor the results of the chromatography purification in real time. The third delivery line 15 is connected to the fourth delivery line 16, and the other end of the fourth delivery line 16 is connected to the waste liquid bottle 7 for collecting waste liquid. Pumps and solenoid valves are installed on the first delivery line 13 and the second delivery line 14. The controlled ends of the pumps and solenoid valves are connected to a PLC controller, and the PLC controller is connected to an audible and visual alarm.

[0025] The first conveying pipeline 13, the second conveying pipeline 14, the third conveying pipeline 15, and the fourth conveying pipeline 16 are made of stainless steel or polytetrafluoroethylene (PTFE). PTFE has strong corrosion resistance, which can extend the service life of the pipeline.

[0026] In practical use, the solution in the temperature control rack 8 is filled according to the temperature conditions. After filling, the stock solution to be purified by chromatography enters the chromatography column 3 through the second pipeline. Under the action of the pressurization mechanism, the sample flow rate in the chromatography column 3 is significantly increased. During the chromatography process, if the temperature sensor 12 in the temperature control rack 8 detects that the temperature exceeds the set value, the PLC controller controls the audible and visual alarm to remind the operator. The operator adds liquid at the appropriate temperature through the liquid inlet 10 of the temperature control rack 8, while the excess liquid is discharged through the liquid outlet 11, thereby adjusting the temperature of the temperature control rack 8 to ensure the smooth progress of the chromatography process. The chromatographically extracted sample enters the receiving bottle 6 through the third delivery pipeline 15, and the waste liquid from the buffer rinsing enters the waste liquid bottle 7 through the fourth delivery pipeline.

Claims

1. A chromatography purification device for chemical production processes, characterized in that: The system includes several buffer bottles (1), each connected to a first delivery line (13). The other end of the first delivery line (13) is connected to a vertically mounted chromatography column (3) with a pressurization chamber. The inlet of the chromatography column (3) is connected to a second delivery line (14), the other end of which is connected to a stock solution bottle (2) containing the solution to be purified by chromatography. The chromatography column (3) is equipped with a pressurization mechanism to accelerate the chromatography process, and the controlled end of the pressurization mechanism is connected to a PLC controller. The chromatography column (3) is equipped with a temperature control frame (8) with a hollow structure for adjusting the temperature of the chromatography column (3), and the temperature control frame (8) is equipped with... There are several vertically arranged column cylinders (9), and the temperature control rack (8) is filled with liquid for heat absorption or heat release; the outlet of the chromatography column (3) is connected to the third delivery pipeline (15), and the other end of the third delivery pipeline (15) is connected to the receiving bottle (6) for receiving samples; the third delivery pipeline (15) is connected to the fourth delivery pipeline (16), and the other end of the fourth delivery pipeline (16) is connected to the waste liquid bottle (7) for collecting waste liquid; the first delivery pipeline (13) and the second delivery pipeline (14) are both equipped with pump bodies and solenoid valves, the controlled ends of the pump bodies and solenoid valves are connected to the PLC controller, and the PLC controller is connected to the audible and visual alarm.

2. The chromatography purification equipment for chemical production processes according to claim 1, characterized in that: The first conveying pipeline (13), the second conveying pipeline (14), the third conveying pipeline (15), and the fourth conveying pipeline (16) are stainless steel pipes or polytetrafluoroethylene pipes.

3. The chromatography purification equipment for chemical production processes according to claim 1, characterized in that: The temperature control rack (8) is made of one of the following materials: plastic, resin, or metal.

4. The chromatography purification equipment for chemical production processes according to claim 3, characterized in that: The temperature control frame (8) has an inlet (10) at the top and an outlet (11) at the bottom.

5. The chromatography purification equipment for chemical production processes according to claim 4, characterized in that: The liquid inside the temperature control frame (8) is water, and a temperature sensor (12) is installed inside the temperature control frame (8). The output of the temperature sensor (12) is connected to the PLC controller.

6. The chromatography purification equipment for chemical production processes according to claim 1, characterized in that: The pressurization mechanism includes a nitrogen cylinder (4), which is connected to a pressurization pipeline; the other end of the pressurization pipeline is connected to the pressurization chamber of the chromatography column (3), and a pressurizer is installed on the pressurization pipeline.

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