Centrifugal device for extracting and detecting soybean urease

Abstract

The utility model discloses a centrifugal device is used in soybean urease extraction and detection, including centrifuge main part and the centrifugal rotor of setting in centrifuge main part, and centrifugal rotor includes transmission shaft and carousel, is provided with the fixed frame on the carousel, and the fixed frame one side is provided with the rotating pin, and the rotating pin is slidably sleeved with the support frame, and the support frame is slidably inserted with the placing seat, and the centrifugal tube is placed in the placing seat, the centrifugal tube bottom is provided with separation component, and separation component includes filter screen and filter membrane, and the centrifugal tube inner wall is provided with the anti -adhesion structure, the centrifugal tube top is provided with the pipe cover, and the centrifugal machine main part is provided with temperature sensor and control unit, the utility model discloses through separation component and anti -adhesion structure, effectively solved the problem of low soybean powder residue interception efficiency and particle adhesion in traditional centrifugal process, improved the separation precision and efficiency of soybean urease extraction.

Description

Technical Field

[0001] This utility model relates to the technical field of soybean urease detection equipment, specifically a centrifuge device for soybean urease extraction and detection. Background Technology

[0002] Soybean urease is the core catalyst in enzyme-induced carbonate precipitation (EICP) technology. The accuracy of its activity detection directly affects the application effect of biomineralization engineering, such as soil solidification and heavy metal remediation. In the current soybean urease extraction and detection process, centrifugation devices are key equipment used to separate soybean flour residues from urease solution to ensure the reliability of subsequent calcium carbonate formation rate detection. However, existing centrifugation devices have significant limitations in practical applications.

[0003] Existing technologies mostly use conventional benchtop centrifuges to achieve solid-liquid separation through high-speed rotation. However, for the specific scenario of soybean urease extraction, its separation efficiency and accuracy are insufficient. Specifically, the traditional centrifugation process has the following problems: First, the retention efficiency of soybean flour residue is low, and the residue in the supernatant after centrifugation is often higher than 1.5%, which leads to a large deviation when the subsequent acid washing method is used to detect precipitated calcium carbonate. Second, during the centrifugation process, soybean flour particles are small in size and have a density close to that of liquid, making it easy to adhere to the filter screen and difficult to separate completely. Utility Model Content

[0004] The purpose of this invention is to provide a centrifuge device for the extraction and detection of soybean urease, which solves the problems of low retention efficiency of soybean flour residue and particle adhesion in traditional centrifugation processes.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a centrifugal device for detecting soybean urease extraction, comprising a centrifuge body and a centrifugal rotor disposed within the centrifuge body, wherein the centrifugal rotor comprises a drive shaft and a turntable, a fixed frame is disposed on the turntable, a pivot pin is disposed on one side of the fixed frame, a support frame is slidably sleeved on the pivot pin, a placement seat is slidably inserted into the support frame, and a centrifugal tube is placed in the placement seat. The centrifuge tube is equipped with a separation component at the bottom, which includes a filter screen and a filter membrane. The centrifuge tube is also equipped with an anti-adhesion structure on its inner wall. The centrifuge tubes are equipped with caps at the top, and a temperature sensor and control unit are installed inside the centrifuge body.

[0006] Preferably, the anti-adhesion structure is a spiral guide groove.

[0007] Preferably, the filter screen is disposed above the filter membrane, and the pore size of the filter screen is larger than the pore size of the filter membrane.

[0008] Preferably, the shape of the placement seat is adapted to the shape of the outer wall of the centrifuge tube, and a rubber buffer layer is provided on the inner side of the placement seat.

[0009] Preferably, the fixed frame and the support frame are hinged together, and the support frame can rotate around the pivot pin.

[0010] Preferably, the control unit is electrically connected to the temperature sensor, and the control unit is used to receive the detection signal from the temperature sensor.

[0011] Preferably, the tube cap is provided with a self-sealing sample dispensing port, which is made of elastic silicone material.

[0012] Compared with the prior art, the beneficial effects of this utility model are: This invention employs a graded separation component consisting of a filter screen and a filter membrane to achieve the step-by-step retention of soybean flour particles from coarse to fine. This design can effectively control the amount of soybean flour residue in the supernatant after centrifugation, fundamentally solving the technical problem of large deviations in subsequent acid washing detection of precipitated calcium carbonate due to excessive residue, and greatly improving the reliability of the detection results.

[0013] This invention features a spiral guide groove structure on the inner wall of the centrifuge tube. This anti-adhesion structure guides the liquid to form a stable spiral flow during centrifugation. This flow pattern can effectively flush away and destroy the adhesion layer of soybean powder particles on the filter screen surface, avoiding the problem of reduced separation efficiency caused by particle blockage, and ensuring a thorough and efficient separation process. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the external structure of the centrifuge device for soybean urease extraction and detection according to this utility model.

[0015] Figure 2 This is a schematic diagram of the internal structure of the centrifuge body of this utility model.

[0016] Figure 3 This is a schematic diagram of the centrifugal rotor of this utility model.

[0017] Figure 4 This is a schematic diagram of the structure of the transfer pin of this utility model.

[0018] Figure 5 This is a schematic diagram of the support frame and placement base of this utility model.

[0019] Figure 6 This is a schematic diagram of the structure of the separation component of this utility model.

[0020] In the diagram: 1-Centrifuge body; 2-Centrifuge rotor; 21-Drive shaft; 22-Turntable; 23-Fixing frame; 24-Turn pin; 25-Support frame; 26-Placement seat; 3-Centrifuge tube; 4-Separation component; 41-Filter screen; 42-Filter membrane; 5-Anti-adhesion structure; 51-Spiral guide groove; 6-Tube cap; 9-Temperature sensor; 10-Control unit. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0022] Please see Figures 1-5 This utility model provides a technical solution: A centrifugal device for soybean urease extraction and detection provided in this embodiment includes a centrifuge body 1 and a centrifugal rotor 2 disposed in the centrifuge body 1. The centrifugal rotor 2 includes a drive shaft 21 and a turntable 22. A drive motor is installed at the bottom of the drive shaft 21 (not shown in the figure). The turntable 22 is welded to the top of the drive shaft 21. A fixing frame 23 is welded to the turntable 22. A pivot pin 24 is welded to one side of the fixing frame 23. A support frame 25 is slidably sleeved on the pivot pin 24. A placement seat 26 is slidably inserted into the support frame 25. A centrifuge tube 3 is placed in the placement seat 26. The drive shaft 21 drives the turntable 22 to rotate, which drives the fixing frame 23 and the support frame 25 on it to rotate synchronously, thereby realizing the centrifugation operation.

[0023] like Figure 6 As shown, a separation component 4 is provided at the bottom of the centrifuge tube 3. The separation component 4 includes a filter screen 41 and a filter membrane 42. Both the filter screen 41 and the filter membrane 42 are fixed on the inner side of the centrifuge tube 3. The filter screen 41 is positioned above the filter membrane 42. The pore size of the filter screen 41 is larger than that of the filter membrane 42. The filter screen 41 is used to initially trap larger soybean powder particles, while the filter membrane 42 is used to finely filter small particles, forming a graded filtration, which effectively reduces the amount of soybean powder residue in the supernatant and avoids subsequent detection deviations.

[0024] like Figure 6 As shown, the inner wall of the centrifuge tube 3 is provided with an anti-adhesion structure 5, which is a spiral guide groove 51, used to guide the liquid to form a spiral flow during centrifugation, reduce the adhesion of soybean powder particles to the filter screen, and improve the separation efficiency.

[0025] like Figure 4 As shown, the shape of the placement seat 26 is adapted to the shape of the outer wall of the centrifuge tube 3. A rubber buffer layer is bonded to the inner side of the placement seat 26 to provide stable support and shock absorption, preventing the centrifuge tube 3 from shaking or breaking during high-speed rotation. The fixing frame 23 and the support frame 25 are hinged together by a pivot pin 24. The support frame 25 can rotate around the pivot pin 24, which makes it easy for the operator to place and take out the centrifuge tube 3, improving the ease of use.

[0026] like Figure 6As shown, the centrifuge tube 3 is provided with a tube cap 6 at the top. The tube cap 6 is provided with a self-sealing sample dispensing port. The self-sealing sample dispensing port is made of elastic silicone material, which facilitates sample dispensing and automatically seals to prevent sample leakage or contamination.

[0027] like Figure 2 As shown, a temperature sensor 9 and a control unit 10 are installed inside the centrifuge body 1. The control unit 10 is electrically connected to the temperature sensor 9. The control unit 10 is used to receive the detection signal from the temperature sensor 9, monitor the temperature in real time during the centrifugation process, and adjust the ambient temperature according to the signal to ensure that soybean urease extraction is carried out under the best conditions and improve the detection accuracy.

[0028] During centrifugation, the centrifuge rotor 2 drives the turntable 22 to rotate at high speed through the transmission shaft 21, which drives the support frame 25 and the placement seat 26 on the fixed frame 23 to rotate synchronously, so that the sample in the centrifuge tube 3 is subjected to centrifugal force to achieve solid-liquid separation. The separation component 4 performs graded filtration through the coordinated filtering of the filter screen 41 and the filter membrane 42. First, the filter screen 41 intercepts larger soybean powder particles, and then the filter membrane 42 finely filters out small particles, significantly reducing the amount of residue in the supernatant. After centrifugation, the centrifuge tube is taken out, and the needle tip of the pipette is directly inserted through the tube opening into the clear liquid layer below the filter membrane 42 to draw out the required supernatant.

[0029] Meanwhile, the spiral guide groove 51 of the anti-adhesion structure 5 guides the liquid to form a spiral flow, destroying the adhesion layer of soybean powder particles on the filter screen, avoiding blockage and residue. The temperature sensor 9 monitors the internal temperature of the centrifuge in real time, and the control unit 10 automatically adjusts the temperature according to the detection signal to maintain the stability of the extraction process. This utility model effectively solves the problems of low soybean powder residue retention efficiency and particle adhesion in traditional centrifugation processes, and improves the separation accuracy and efficiency of soybean urease extraction.

[0030] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A centrifugal device for detecting soybean urease extraction, comprising a centrifuge body (1) and a centrifugal rotor (2) disposed within the centrifuge body (1), characterized in that: The centrifugal rotor (2) includes a drive shaft (21) and a turntable (22). A fixed frame (23) is provided on the turntable (22). A pivot pin (24) is provided on one side of the fixed frame (23). A support frame (25) is slidably sleeved on the pivot pin (24). A placement seat (26) is slidably inserted on the support frame (25). A centrifugal tube (3) is placed in the placement seat (26). The centrifuge tube (3) is provided with a separation component (4) at the bottom. The separation component (4) includes a filter screen (41) and a filter membrane (42). The centrifuge tube (3) is provided with an anti-adhesion structure (5) on its inner wall. The centrifuge tube (3) is provided with a tube cap (6) at the top, and a temperature sensor (9) and a control unit (10) are provided inside the centrifuge body (1).

2. The centrifuge device for soybean urease extraction and detection according to claim 1, characterized in that: The anti-adhesion structure (5) is a spiral guide groove (51).

3. The centrifuge device for soybean urease extraction and detection according to claim 1, characterized in that: The filter screen (41) is disposed above the filter membrane (42), and the pore size of the filter screen (41) is larger than the pore size of the filter membrane (42).

4. The centrifuge device for soybean urease extraction and detection according to claim 1, characterized in that: The shape of the placement seat (26) is adapted to the shape of the outer wall of the centrifuge tube (3), and a rubber buffer layer is provided on the inner side of the placement seat (26).

5. The centrifuge device for soybean urease extraction and detection according to claim 1, characterized in that: The fixed frame (23) is hinged to the support frame (25), and the support frame (25) can rotate around the pivot pin (24).

6. The centrifuge device for soybean urease extraction and detection according to claim 1, characterized in that: The control unit (10) is electrically connected to the temperature sensor (9), and the control unit (10) is used to receive the detection signal from the temperature sensor (9).

7. The centrifuge device for soybean urease extraction and detection according to claim 1, characterized in that: The tube cap (6) is provided with a self-sealing sample dispensing port, which is made of elastic silicone material.

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