MICE EXPRESSING A LIMITED REPERTORY OF IMMUNOGLOBIN LIGHT CHAINS

Abstract

A genetically modified mouse is provided, wherein the mouse expresses a repertoire of immunoglobulin light chains characterized by a limited number of light chain variable domains. Mice are provided that exhibit a selection of two human light chain variable region gene segments, such that the immunoglobulin light chains expressed by the mouse comprise one of the two human light chain variable region gene segments. Methods are provided for generating bispecific antibodies having universal light chains using mice described herein, comprising the human light chain variable regions. Methods are provided for generating human variable regions suitable for use in multispecific binding proteins, e.g. bispecific antibodies, as well as host cells.