CHEMICALLY ENCODED AND SPATIALLY LOCATED LIBRARIES SCREENING PLATFORMS

Abstract

Encoded separation pool libraries are provided herein, which are useful, inter alia, in forming highly diverse and dense arrays for screening and detecting a variety of molecules. A method of immobilizing a microparticle on a solid support is disclosed, wherein the microparticle is covalently linked via first and second linkers to a linker domain (e.g., peptide, protein, small molecule) and a nucleic acid, and performing a decoding process on the nucleic acid domain, thereby determining the composition of the linker domain and its location on said solid support. The second linker is selectively cleavable provided that the first linker is not cleavable. The decoding may include hybridization or sequencing. The second linker is e.g. a photocleavable linker or includes -S(O)2NH- -NHS(O)2- -C(O)O-, -OC(O)-, -C(O)NH-, -NHC(O)-.The linker domain may be bound to a linker ligand (which may be fluorescent), unless protected by a protecting group, which may be removed.