METHOD FOR LIGHT MICROSCOPIC IMAGE OF A SAMPLE, DEVICE AND COMPUTER PROGRAM

Abstract

The invention relates to a method for light microscopic imaging of a sample, wherein a scanning device scans at least one illumination focus over or through the sample during a pre-scan of a sample area, wherein light emissions from emitters in the sample are detected by a detector during the pre-scan, wherein the light emissions are assigned target positions of the at least one illumination focus in the sample, and wherein actual positions of the at least one illumination focus in the sample, assigned to the target positions, are detected by a measuring device during the pre-scan, and wherein a subsequent step is carried out within a sub-area of ​​the sample area.in which a position in the sample area depicted by the pre-scan is controlled based on the recorded actual positions, or image processing is carried out based on the light emissions and the assigned actual positions, and / or at least one measured value is determined, as well as devices and computer programs for carrying out the procedure.

Images