Flow injection and isolation combination valve for high-pressure fluids

Abstract

Flow injection valve (300; 850), the flow injection valve comprising: a stationary element (802'); a movable element (11; 804'), wherein a surface of the stationary element contacts a surface (810') of the movable element, the movable element comprising several flow lines (16, 28, 44, 50; 876', 886', 890', 898'), each of the several flow lines having at least one opening (14, 30, 40, 42, 46, 48; 860', 874', 882', 892', 894', 896') on the surface of the movable element; and at least one pin isolation valve (1, 2, 3, 4); wherein the at least one pin isolation valve has a pin and an internal flow line (58', 96', 812', 858') through the pin, wherein the at least one pin isolation valve is movably arranged such that the pin can be actuated to connect with one of the first of the several flow lines in the movable element (11;804') to communicate fluidically, wherein at least one pin isolation valve can be actuated in such a way that it moves away from the movable element (11; 804') and is repositioned towards it in order to communicate fluidically with a second of the several flow lines in the movable element (11; 804').

Description

This application claims priority to US Provisional Patent Application No. 60 / 550,930 filed March 5, 2004. Reference is hereby made to the content of this application. BACKGROUND OF THE INVENTION The invention relates generally to the field of high pressure fluids and more particularly to a combination of multiple isolation valves, which allows a flow path to be introduced without interrupting flow from the fluid source. STATE OF THE ART Conventional 6-port face shear valves, also known as face seal valves valves) and used in high pressure liquid chromatography (HPLC), provide connectors that connect to the sample, syringe, pump, column, and the two ends of the sample loop. such Mechanical seal valves must be rotated or turned to move from one port to another. The rotation of Mechanical seal under high pressure inherently causes damage to the contacting plastic surfaces as the fluid port openings move against the rotor surface, causing wear of the rotor material. This leads to a shortened lifespan of the mechanical seal valve. Moreover, it is necessary to temporarily block the flow during the sample injection process, and a Sample dispersion occurs. At higher chromatographic pressures, for example greater than 15,000 psig or 100 MPa, there is a need for one Flow-through isolation sample injection valve that provides long sample injection life with minimal sample distortion and a minimal Has pump pressure pulsation. BRIEF SUMMARY OF THE INVENTION In order to solve the above and other problems, the present invention provides a combination of several Flow-through high-pressure isolation valves for high-pressure fluids, particularly for use in HPLC applications as a replacement for the conventional sliding ring shear valve is suitable. It is an object of the invention to provide a flow-through sample injection valve with fluid port openings that do not slide against each other move a rotor surface, leading to wear of the rotor material. It is another object of the invention to provide a flow-through sample injection valve that allows flow of sample through non-cylindrical Avoids passes to minimize sample dispersion. According to a particular aspect of the invention, the present invention relates to a flow-through injection valve, wherein the Flow injection valve comprising: a stationary element; a movable element, wherein a surface of the stationary element has a touches the surface of the moving element; and at least one pin isolation valve. The at least one pin isolation valve has an internal Flow line and is movably arranged so that the internal line is adapted to be fluidly connected to at least one flow line to communicate in the movable element, and is movably arranged so that the internal line is adapted to communicate with another Flow line to communicate fluidly in the movable element. The movable element of the flow injection valve can further first and second conduits for connection to internal conduits of the first and second pin isolation valves, wherein the first and second lines terminate in a surface of the moveable member; a third line, the fluidic communication between allowing the internal conduits of the first and second pin isolation valves; and a fourth line, the fluidic communication enabling internal conduits of the third and fourth pin isolation valves, the third pin isolation valve providing fluid flow, and the fourth pin isolation valve vents fluid flow. The movable element can rotate about an axis of rotation or by move a linear movement and / or a curved movement. One of the pin isolation valves may be fluidly connected to a sample loop. a sample circuit of a high pressure liquid chromatography system (HPLC system) to be connected. One of the pin isolation valves may be in fluid communication with a pump that supplies high pressure fluid to a High pressure liquid chromatography system promotes. One of the pin isolation valves may be fluidly connected to a column through which High pressure liquid is drained from a high pressure liquid chromatography system. According to a particular aspect of the invention, the present invention relates to a flow-through injection valve, wherein the flow-through injection valve is arranged about an axis of rotation, the injection valve comprising: at least two opposite valve ends arranged about the axis of rotation; wherein the movable element comprises a rotor between the Valve ends is arranged, wherein an axis of rotation of the rotor is parallel to the axis of rotation of the injection valve and / or with this coincides and the rotor is arranged such that the orientation of the rotor is changed by rotation about the axis of rotation of the rotor can be. The rotor has an outside, at least two opposing surfaces each intersecting the outside, one first flow conduit having an opening on a first of the at least two opposing surfaces and an opening on a second having at least two opposing surfaces, a second flow conduit having an opening on a first of the at least having two opposing surfaces and an opening on a second of the at least two opposing surfaces; one Flow conduit having an opening on the outside and an opening on the first of the at least two opposing surfaces, and a flow conduit having an opening on the outside and an opening on the second of the at least two opposing surfaces having. The rotor further includes a first sealing ring for sealing the openings on the first of the at least two opposite ones surfaces and a second sealing ring for sealing the opening on the second of the at least two opposing surfaces. The rotor further includes a first pin isolation valve having an internal conduit, the first pin isolation valve being positioned to move parallel to the axis of rotation of the injection valve, the first pin isolation valve being movably arranged such that that this is adapted to be fluidly connected via the internal conduit with the opening on the first flow channel on the first of the at least two to communicate fluidly opposite surfaces, and is movably arranged such that this is suitable for the internal Line with the flow line having an opening on the outside and an opening on the second of the at least two opposite ones To communicate fluidly on surfaces. The rotor further includes a second pin isolation valve having an internal conduit, the second pin isolation valve is arranged to move parallel to the axis of rotation of the injection valve and is movably arranged such that it is able to communicate fluidly via the internal conduit with the opening on the first flow channel on the second of the at least two opposite surfaces to communicate, and is movably arranged so as to be able to communicate via the internal line fluidly communicating with the flow line having an opening on the outside and an opening on the second of the at least two has opposite surfaces. The rotor further includes a third pin isolation valve having an internal conduit locating the third pin isolation valve is to move parallel to the centerline of the injection valve, wherein the third pin isolation valve is movably arranged such that this being adapted to be fluidly connected via the internal conduit to the opening on the second flow channel on the first of the at least two to communicate fluidly opposite surfaces, and is arranged to be movable in such a way that this is suitable for the internal Line to fluidly communicate with the flow line having an opening on the outside and an opening on the second of the at least has two opposite surfaces. The rotor further includes a fourth pin isolation valve having an internal conduit wherein the fourth pin isolation valve is arranged to move parallel to the centerline of the injection valve, wherein the fourth pin isolation valve is movably arranged in such a way that it is suitable for fluidly communicating with the opening on the second flow channel via the internal line the second of the at least two opposing surfaces, and is movably arranged to be capable of doing so is to fluidly communicate with the flow line via the internal line having an opening on the outside and an opening on the second having at least two opposing surfaces. The rotor may further include: a rotor clamp having an outside and an inside, wherein the inside has at least one Surrounding portion of the outside of the rotor; a first opening on the outside of the rotor clamp, penetrating through the rotor clamp to coincide with the first opening on the outside of the rotor; and a second opening on the outside of the rotor clamp, the penetrating the rotor clamp to coincide with the second opening on the outside of the rotor. The rotor clamp can also drive means for driving the rotor to rotate about the axis of rotation of the rotor. The rotor clamp drive means can be a Drive transmission actuation unit (gear drive operator) or a handle actuation unit (handle operator) include. At least one of the valve ends may include: a stator surrounding the at least one pin isolation valve, the stator being attached to the Ro gate adjacent; a gasket layer surrounded in the stator and surrounding the at least one pin isolation valve to seal the at least one pin isolation valve; a Belleville spring washer; a Belleville feather; a load seal washer; and a ball nut, wherein the Belleville spring washer, the Belleville spring, the load washer and the ball nut are arranged axially to apply an axial force to seal the gasket layer surrounding the pin isolation valve. The first and / or the second Pin isolation valves can be fluidly connected to a sample loop of a high pressure liquid chromatography (HPLC) system being. The third and / or fourth pin isolation valve may be in fluid communication with a pump that supplies high pressure fluid a high pressure liquid chromatography (HPLC) system, or in fluidic communication with a column through which High pressure liquid is discharged from a high pressure liquid chromatography (HPLC) system. According to another embodiment, the present invention relates to a multiple valve comprising: a housing; a Rotary flow isolation valve disposed in the housing, the isolation valve for isolation of fluid flow in a axially oriented, the isolation valve being disposed about an axis of rotation, the isolation valve comprising: at least two opposite valve ends arranged about the axis of rotation; a rotor which is arranged between the valve ends, wherein a The axis of rotation of the rotor is substantially parallel to and coincident with the axis of rotation of the isolation valve, wherein the Rotor is arranged such that the orientation of the rotor can be changed by rotation about the axis of rotation of the rotor. the Rotor has: an outer surface, at least two opposing surfaces each intersecting the outer surface, a flow conduit, the one opening on a first of the at least two opposing surfaces and one opening on a second of the at least two having opposite surfaces; a flow line having an opening on the outside and an opening on the first of the at least has two opposite surfaces; a flow line having an opening on the outside and an opening on the second der has at least two opposing surfaces; at least one blind opening on the first of the at least two opposite ones Surfaces; and at least one blind opening on the second of the at least two opposing surfaces. The rotor also includes: a first gasket for sealing the openings on the first of the at least two opposing surfaces; and a second gasket for sealing the openings on the second of the at least two opposing surfaces. The rotor also includes: a first pin isolation valve, the first pin isolation valve being arranged to rotate along the axis of rotation of the isolation valve move, wherein the first pin isolation valve is movably arranged such that it is adapted to be fluidly connected to the at least one to communicate blind opening on the first of the at least two opposite surfaces, and is movably arranged such that this is adapted to fluidly communicate with the flow line having an opening on the outside and an opening on a second of the at least two opposing surfaces; and a second pin isolation valve, the second pin isolation valve arranged to move along the centerline of the isolation valve, the second pin isolation valve being so movably arranged is that it is capable of being fluidly connected to the at least one blind port on the second of the at least two opposite ones To communicate surfaces, wherein the second pin isolation valve is movably arranged such that this is adapted to fluidly with the To communicate flow line having an opening on the outside and an opening on the second of the at least two opposite has surfaces. The multiple valve further includes: a linear flow injection valve, wherein the injection valve includes: a stationary Element; a moveable element, the stationary element and the moveable element touching at a surface, the moveable element arranged to slide along the surface; a chamber between the stationary element and the movable element is arranged with the chamber defined by the area; wherein the moveable element has a first flow line having a first Having opening, which is connected to the chamber, and a second opening on a surface of the movable member, which is not connected to the Chamber is connected, wherein the movable element has a second flow line having a first opening connected to the chamber and a second opening on a surface of the moveable member not connected to the chamber. The movable The element further comprises: a third flow line having a first opening and a second opening, each on a surface of the movable elements connected to the chamber; and a fourth flow line having a first opening and a second opening each terminating on a surface of the movable element which is connected to the chamber. In yet another embodiment, be the present invention relates to a multiple valve comprising: a housing; a linear flow isolation valve disposed within the housing wherein the isolation valve comprises: a stationary member; a movable element, the stationary element and the movable element touching at a surface, the moveable element being arranged to slide along the surface; a chamber between the stationary element and the movable element, the chamber being delimited by the area. The moving element has a first flow line having an opening connected to the chamber and an opening on a surface of the movable member not connected to the chamber, a second flow line having an opening communicating with the chamber and an opening on a surface of the movable member not connected to the chamber, a first blind opening on the surface delimiting the chamber and a second blind opening on the surface delimiting the chamber. The multiple valve also includes a. linear flow injection valve, the injection valve comprising: a stationary element; a moving element, where the stationary element and the movable element touch at a surface, the movable element being arranged to move along the surface to slide; and a chamber disposed between the stationary member and the movable member, the chamber being formed by the area is limited. The moveable element includes: a first flow line having a first opening connected to the chamber and a second opening on a surface of the moveable element not connected to the chamber, a second flow line, having a first opening connected to the chamber, and a second opening on a surface of the movable element, the not connected to the chamber, a third flow line having a first opening and a second opening each on a surface of the having movable elements connected to the chamber, and a fourth flow line having a first opening and a second Opening each having on a surface of the movable element, which are connected to the chamber. The linear flow injection valve of the multiple valve may further comprise: at least one (a) first pin isolation valve; (b) second pin isolation valve, (c) third pin isolation valve and (d) fourth pin isolation valve; wherein the first pin isolation valve has an internal line wherein the first pin isolation valve is disposed within an opening within the stationary member communicating with the chamber is connected such that the internal conduit of the first pin isolation valve is movably arranged to be in fluidic communication with the first Opening to stand on a first flow line of the movable member, and movably arranged to be in fluidic communication with the first opening of the third flow line, the second pin isolation valve having an internal line, the second Pin isolation valve is located within an opening within the stationary member, which is connected to the chamber so that the internal conduit of the second pin isolation valve is movably disposed to be in fluid communication with the first port on a second flow line of the moveable member and is movably arranged to be in fluidic communication with the second port of the third flow line, the third pin isolation valve having an internal line, the third pin isolation valve is arranged within an opening within the stationary element, which is connected to the chamber, so that the internal conduit of the third pin isolation valve movably disposed to be in fluid communication with the first port of the fourth flow line and movably arranged to be in fluid communication with the first opening of the first flow line. The fourth Pin isolation valve has an internal conduit with the fourth pin isolation valve within an opening within the stationary member which is connected to the chamber so that the internal conduit of the fourth pin isolation valve is movably arranged to to be in fluidic communication with the second opening of the fourth flow line, and movably arranged to be in fluidic to be in communication with the first opening of the second flow line. Those skilled in the art will recognize that any combination of multiple valves can be made, such as rotary isolation and linear injection, linear isolation and rotary injection, rotary injection and rotary isolation and linear injection and linear isolation. Further, each rotary injection, rotary isolation, linear injection and linear isolation valve can be manufactured independently will. The present invention further relates to a method of operating a flow-through injection valve, the valve comprising: a movable element, wherein the movable element has a first and a second line for connection to internal lines of a first and a second pin isolation valve, the first and second conduits terminating in a surface of the moveable member; a third conduit enabling fluidic communication between the internal conduits of the first and second pin isolation valves; a fourth Line that fluidic communication between the internal lines of the third ten and the fourth pin isolation valve allows, wherein the third pin isolation valve provides fluid flow and the fourth pin isolation valve the omits fluid flow; (A) with the valve in an initial position of flow isolation so that the third pin isolation valve, fluid flow provides is in fluid communication with the fourth pin isolation valve venting fluid flow, the first pin isolation valve in in fluid communication with the first line and the second pin isolation valve in fluid communication with the second line;the method comprising the steps of: (I) wherein the first pin isolation valve is connected to the first line, (1) moving the first pin isolation valve away from the first line; (2) moving the movable member, (3) moving the first pin isolation valve in direction of the movable member so that the internal line within the first pin isolation valve is connected to the third line; and (II) the second pin isolation valve being connected to the second conduit, (1) moving the second pin isolation valve away from the second Management; (2) moving the movable member, (3) moving the second pin isolation valve toward the movable member so that the internal line within the second pin isolation valve is connected to the third line to thus fluidic communication between to fabricate the first and second pin isolation valves; and (III) wherein the third pin isolation valve is connected to the fourth line (1) moving the third pin isolation valve away from the fourth conduit, (2) moving the moveable member; (3) Moving the third Pin isolation valve toward the first conduit for fluidic communication with the internal conduit of the third pin isolation valve to manufacture; and (IV) wherein the fourth pin isolation valve is connected to the fourth line, (1) moving the fourth pin isolation valve away from the fourth line; (2) moving the movable element; (3) moving the fourth pin isolation valve towards the second line, to establish fluidic communication with the internal conduit of the fourth pin isolation valve; and (B) the valve being in a initial position of the flow passage such that at least (a) the third pin isolation valve providing fluid flow communicates with the first conduit and / or (b) the fourth pin isolation valve venting fluid flow is connected to the second conduit, the method comprising the steps of: (III) connecting the third pin isolation valve to the first conduit, (1) moving the third pin isolation valve away from the first conduit, (2) moving the moveable member, and (3) moving the third pin isolation valve toward the moveable Elements such that the internal line within the third pin isolation valve is connected to the fourth line; and (IV) wherein the fourth pin isolation valve is connected to the second line, (1) moving the fourth pin isolation valve away from the second line, (2) moving of the movable member and (3) moving the fourth pin isolation valve toward the movable member so that the internal conduit connected to the first line within the second pin isolation valve; (V) wherein the first pin isolation valve with the third line is connected, (1) moving the first pin isolation valve away from the third conduit, (2) moving the moveable member, and (3) moving of the first pin isolation valve toward the movable member so that the internal line within the first pin isolation valve with connected to the first line; and (VI) wherein the second pin isolation valve is connected to the third line, (1) moving the second pin isolation valve away from the third line, (2) moving the moveable member, and (3) moving the second pin isolation valve in Direction of the movable member so that the internal line within the second pin isolation valve is connected to the second line. In a further embodiment according to the present invention, the present invention also relates to a method for Operating a multi-valve, the multi-valve comprising a flow-through isolation valve, the flow-through isolation valve comprising: a movable element, wherein the movable element has a first and a second line for connection to internal lines of the first and the second pin isolation valve, the conduits terminating in a surface of the moveable member; first and second blind openings for connection to the internal conduits of the first and second pin isolation valves, (A) the valve being in an initial position of flow isolation such that at least (a) the first pin isolation valve providing fluid flow is connected to the first blind port and / or (b) the second pin isolation valve venting the fluid flow is connected to the second blind port, the method comprising the Steps includes: (I) connecting the first pin isolation valve to the first blind port, (1) moving the first pin isolation valve away from the first blind orifice, (2) moving the moveable member, and (3) moving the first pin isolation valve toward the moveable Elements such that the internal line within the first pin isolation valve is connected to the first line formed in a surface of moving element ends; and (II) wherein the second pin isolation valve is connected to the second blind port, (1) moving the second pin isolation valve away from the second blind orifice, (2) moving the moveable member, and (3) moving the second pin isolation valve in direction of the be movable element, so that the internal line is connected within the second pin isolation valve with the second line, which in a Surface of the movable member ends, and (B) wherein the valve is in an initial position of the flow passage so that at least (a) the first pin isolation valve providing fluid flow is connected to the first conduit and / or (b) the second pin isolation valve providing exhausting fluid flow, is connected to the second conduit, the method comprising the steps of: (III) wherein the first pin isolation valve connected to the first line, (1) moving the first pin isolation valve away from the first line, (2) moving the movable one element and (3) moving the first pin isolation valve toward the movable element such that the internal conduit within the first pin isolation valve is connected to the first blind port; and (IV) wherein the second pin isolation valve is connected to the second line, (1) moving the second pin isolation valve away from the second conduit, (2) moving the moveable member, and (3) moving the second Pin isolation valve toward the movable member so that the internal line within the second pin isolation valve with the second blind opening is connected. In the method of operating a flow-through injection valve, the first and second conduits formed in a surface of the moving element, in fluidic communication with a sample loop of a high pressure liquid chromatography (HPLC) system Systems) or the first and second pin isolation valves may be in fluid communication with a needle and syringe of a High pressure liquid chromatography system (HPLC system) are located or the third and fourth pin isolation valve can be in fluidic In communication with a pump and a column of a high pressure liquid chromatography (HPLC) system. In the method of operating a multi-valve, the multi-valve also includes a flow-through injection valve, wherein the A flow-through injection valve comprises: a moveable member, the moveable member having first and second conduits for communication having internal conduits of a first and a second pin isolation valve, the first and second conduits being in a surface of the moving element ends; a third conduit providing fluidic communication between the internal conduits of the first and second pin isolation valve allows; a fourth conduit providing fluidic communication between the internal conduits of the third and fourth pin isolation valve allows, wherein the third pin isolation valve provides fluid flow and the fourth pin isolation valve exhausts the fluid flow; (A) with the valve in an initial flow isolation position such that the third pin isolation valve providing fluid flow is in is in fluid communication with the fourth pin isolation valve venting fluid flow, the first pin isolation valve being in fluid communication is in communication with the first line and the second pin isolation valve is in fluid communication with the second line; the method comprising the steps of: (I) connecting the first pin isolation valve to the first conduit, (1) moving the first pin isolation valve away from the first line; (2) moving the movable member, (3) moving the first pin isolation valve toward the movable element; such that the internal line within the first pin isolation valve is connected to the third line; and (II) where the second pin isolation valve is connected to the second line, (1) moving the second pin isolation valve away from the second line; (2) moving the movable member, (3) moving the second pin isolation valve toward the movable member so that the internal Conduit within the second pin isolation valve is connected to the third conduit so as to provide fluidic communication between the first and to manufacture the second pin isolation valve; and (III) wherein the third pin isolation valve is connected to the fourth line, (1) moving the third pin isolation valve away from the fourth line; (2) moving the movable element; (3) Move the third pin isolation valve toward the first conduit to establish fluidic communication with the internal conduit of the third pin isolation valve; and (IV) where the fourth pin isolation valve is connected to the fourth conduit, (1) moving the fourth pin isolation valve away from the fourth conduit; (2) moving the movable member; (3) moving the fourth pin isolation valve toward the second conduit to provide fluid communication to connect to the internal line of the fourth pin isolation valve. The method of operating a multiple valve also includes the steps of: (B) wherein the valve is in an initial position of flow passage such that at least (a) the third pin isolation valve providing fluid flow is connected to the first conduit and / or (b) the fourth pin isolation valve venting fluid flow is connected to the second line, the method comprising the steps of: (III) wherein the third pin isolation valve is connected to the first line, (1) moving the third pin isolation valve away from the first line, (2) moving the movable member; and (3) moving the third pin isolation valve toward the movable member so that the internal management within the third th pin isolation valve is connected to the fourth line; and (IV) wherein the fourth pin isolation valve is connected to the second line, (1) moving the fourth pin isolation valve away from the second line, (2) moving the moveable member, and (3) moving the fourth Pin isolation valve toward the movable member so that the internal line within the second pin isolation valve with the first line is connected; and (V) wherein the first pin isolation valve is connected to the third line, (1) moving the first pin isolation valve away from the third line, (2) moving the moveable member, and (3) moving the first pin isolation valve toward the moveable Elements such that the internal line within the first pin isolation valve is connected to the first line; and (VI) where the second pin isolation valve is connected to the third line, (1) moving the second pin isolation valve away from the third line, (2) moving of the movable member and (3) moving the second pin isolation valve toward the movable member so that the internal conduit connected to the second conduit within the second pin isolation valve. In the method of operating a multi-valve, the first and second lines formed in a surface of the movable elements of the flow-through injection valve, in fluid communication with a sample loop of a high pressure liquid chromatography Systems (HPLC systems) are or the first and the second pin isolation valve of the flow injection valve are in fluidic Communication with a needle and a syringe of a high pressure liquid chromatography (HPLC) system or the third and the fourth pin isolation valve of the flow-through injection valve may be in fluid communication with a pump and a column of a High Pressure Liquid Chromatography (HPLC) systems. BRIEF DESCRIPTION OF THE DRAWINGS These and other features and advantages of the present invention will become more apparent from the following description and the accompanying drawings, in which drawings like reference numerals indicate like structures. Figure 1A shows a combination of multiple rotary flow isolation valves according to the present invention in one Side elevational cross-sectional view in the loading position connected to a sample loop. Figure 1B shows the combination of multi-flow rotary isolation valves according to the present invention of Figure 1A in one Transition positions associated with a sample loop. Figure 1C shows the combination of multi-flow rotary isolation valves according to the present invention of Figure 1A in one Injection positions connected to a sample loop. Figure 2A shows a partially cut-away perspective view of the insulation section of the combination of multiple Flow-through rotary isolation valves of FIGS. 1C Fig. 2B shows an exploded perspective view of the rotor of the insulation section of the multi- flow-through rotary isolation valve of FIGS. 1C 3A shows a top view of the housing of the multiple rotary flow isolation valves of FIGS. 1A-1A. 1C 3B shows an elevational view of the housing of the multi-flow rotary isolation valves of FIGS. 1A-1A. 1C Figure 3C shows a detailed view of a portion of the rotary isolation valve assembly of Figure 3A. FIG. 4A shows a cross-sectional view separated at break lines 4B of another embodiment of a multiple valve according to FIG present invention consisting of a linear isolation valve and a linear injection valve. Figure 4B shows a cross-sectional view of the embodiment of Figure 4B separated at break lines 4A. DETAILED DESCRIPTION OF THE INVENTION The present invention describes a combination isolation valve of multiple flow high pressure isolation valves. several such valves for high pressure fluids. The combination isolation valve can replace the traditional face shear valves used in HPLC systems are used. The rotors are designed for use in high pressure fluid systems to allow switching to another Allow flow path without temporarily blocking flow as occurs with face shear valves such as those conventionally used High pressure fluid systems and in particular in high pressure liquid chromatography. Of the Sample fluid injection circuit can be isolated from the rest of the HPLC system. Each of the combinations of several or Multi-flow isolation valves include a housing having a bore therethrough and a cylindrical rotor located within of the bore can be rotated. When the isolation rotor is in its fluid flow position during the injection phase, fluid flows from a pump across the Isolation valve to the sample injection circuit back through another port of the valve and then to a column. By turning the rotor around rotated 90°, the fluid stop fittings prevent the flow of fluid and isolate or isolate the sample loop from the rest of the HPLC system. systems. In Figure 1A, an injection rotor 11 of an injection valve 300 is a combination of isolation valves or multiple isolation valves and a multiple isolation valve 10 in a loading phase in fluid communication with a needle 12 to a valve pin 1 at a Port 14 shown on one side of rotor 11. The combination isolation valve or multiple isolation valve 10 includes a isolation valve 200 and the injection valve 300 . Sample fluid flows from the pen 1 into the internal conduit 16 . The sample fluid usually flows through a bend 15 of substantially 90° to a port 18 on the outside of the rotor 11 . Port 18 is preferred Fluidly connected to a flexible inlet tube 20 and correspondingly to the sample loop 22 so that sample fluid enters the sample loop 22 flows in and flows out through a flexible outlet pipe 24 . The flexible outlet tube 24 is preferably fluidly connected to a port 26 located on the outside of the rotor 11 via a valve pin 2 and in turn with an internal line 28 . The sample fluid usually flows through an im Substantially 90° bend 29 to a port 30 on an opposite side of the rotor 11 . A syringe 32 can be fluidly connected to the Port 30 may be connected such that a negative pressure is created within flexible tubing 24, sample loop 22, flexible tubing 20 and needle 12 to draw sample fluid and allow sample fluid to be drawn into sample loop 22.

[0039] Injection rotor 11 comprises ports 40 and 42 connected via internal channel 44; and terminals 46 and 48, the are connected to each other via the internal channel 50. An annular space 52 is formed on one side of the rotor 11, the one fluidic communication between ports 14 , 40 and 46 provides. An annular space 54 is on the opposite side Formed side of the rotor 11, which provides fluidic communication between the ports 30, 42 and 48. During the loading phase, the injection rotor 11 is separated from the high-pressure pump 101 and the column 102 by an isolation rotor 61 isolated or separated. The two rotors 11 and 61 are connected to one another via high-pressure tubing 36 and 38 . In particular, the High pressure tubing 36 is fluidly connected to internal passage 50 via valve pin 3 at port 46 while the high pressure tubing 38 is fluidically connected to the internal channel 50 via valve pin 4. The high pressure tubing 36 is fluidly connected to the isolation rotor 61 connected to the valve pin 5 which is connected to the rotor 61 at the blind port 82. Accordingly, the high pressure tubing 38 is fluidic connected to the isolation rotor 61 via the valve pin 6, which is connected to the rotor 61 at the blind port 84. therefore corresponds to during the loading phase, the pressure within the high pressure tubing 36 and 38 is substantially atmospheric, i.e., 0 psig or 0.101 MPa absolute. The isolation rotor 61 includes ports 62 and 64 that are interconnected via the internal passage 66 . The high pressure pump 101 is fluidically connected to port 70 via an internal passage 74 . The pump 101 is with the outside of the rotor 61 at one Port 78 connected. Likewise, the column 102 is fluidly connected to the port 72 via an internal passage 76 . The pillar 102 is connected to the outside of the rotor 61 at a port 80 . The injection rotor 11 may include a wash pump connected to port 40 and a wash outlet, connected to port 42. Since stagnant flow can occur in the internal rotor passageway 44, the Rotor wash supply connection 110 provided to be connected to a separate wash pump (not shown) while the Wash drain connection 112 allows draining or draining the used wash solution. The wash pump washes the internal chamber 44 after sample injection. An annular space 88 is on one side of the rotor 61 formed, which provides fluidic communication between the ports 62 and 70. An annular gap 90 is at the opposite side of rotor 61 which provides fluidic communication between ports 64 and 72. Figure 1B shows the transition phase between loading the sample into the sample loop 22 and the injection phase, in which the sample is injected within the loop 22 by the high-pressure pump 101. During this transition period the isolation rotor 61 remains in the same position as during the charging phase. Only the orientation of the injection rotor 11 is changed. In particular, the rotor 11 is rotated such that the valve pins 1 and 2 are no longer connected to the sample loop 22, and thus the needle 12 and the syringe tube 32 from the sample loop 22 or to separate. The valve pin 1 is inserted into the port 40 while the valve pin 2 is inserted into the port 42 so that the needle and syringe are fluidly connected to each other via the internal line 44 are. Figure 1C shows the injection phase when high pressure liquid is supplied from pump 101 to sample loop 22 and column 102 will. In particular, during the injection phase, the rotor 11 remains in the position it assumed during the transition phase. Of the Rotor 61 is rotated so that valve pins 5 and 6 are no longer connected to blind ports 82 and 84, respectively. The valve pin 5 is now connected to port 70 to effect fluid communication between pump 101 and high pressure tubing 36 . Accordingly, the valve pin 6 is now connected to the connection 72 in order to establish fluidic communication between the high-pressure tubing 38 and the column 102. The annular spaces 88 and 90 formed on opposite sides of the rotor 61 constitute a High pressure sealing ring for the rotor 61 ready. Those skilled in the art will recognize that after the injection phase illustrated in Figure 1C, the flow-through isolation valve 200 and the Flow injection valve 300 can be returned to the loading phase by returning to the transition phase shown in FIG. 1B 1A and then reversed to the charging phase shown in FIG. 1A. Fig. 2A shows a perspective view of the isolation rotor 61 as it is placed within a valve body to a Form valve assembly 200. The components of the valve assembly 200 are typically rotated about an axis, such as the centerline 200CL, centered. In particular, the rotor 61 is arranged such that the stators 202 and 204 are at each end of the rotor 61 are arranged. Belleville springs 224 and 222 redirect the axial loads applied to rotor 61 along centerline 200CL will. Belleville springs 220 and 222 and Belleville washers 232 and 234 are at one end of both stators 202 and 204 attached by means of flanges 224 and 226. Load seal washers 224 and 226 are held in position by ball nuts 228 and 230 locked. Both sets of gasket layers 206 and 208 are compressed by the axial forces created by the Belleville Spring washers 232 and 234 are applied. The rotor 61 is preferably made of PEEK (polyetheretherketone) or a PEEK Mixture. The rotor clamp 240 and the stators 202 and 204 are preferably made of type 316 stainless steel. Those skilled in the art will appreciate that the present invention is not limited to the above materials, but others Materials can also be used. The rotor 61 is shown in a cross-sectional view as positioned between the stators 202 and 204. As shown in FIG. The rotor 61 is sealed by a set of three gasket layers 206 and 208 fitted around valve pins 5 and 6, respectively. The preferred materials for the Gasket layers 206 and 208 include PEEK, PTFE (polytetrafluoroethylene), PEEK, in that order. Those skilled in the art will appreciate that the present invention is not limited to the above materials and that others Materials can also be used.

[0052] The respective ends 242 and 244 of the flow-through isolation valve 200 thus effectively comprise the stators 202 and 204 which Seal layers 206 and 208, the Belleville spring washers 232 and 234, the Belleville springs 220 and 222, the load washers 224 and 226 and ball nuts 228 and 230 . Figure 2B shows an exploded view of a portion of the components constituting a first variation of the embodiment of the Flow isolation valve 200 includes. Pump delivery fitting 101 connects to port 78 in rotor 61 and fitting 102 from the outlet feed to the column is connected to port 80 in rotor 61 . The face seal valve feed pin 6 is from the stator 204 and connected to one end of the rotor 61, while the face seal valve drain pin 5 is surrounded by the stator 202 and connected to the opposite end of the rotor 61 . During normal operation, only pins 5 and 6, which are Stators 202 and 204 are surrounded, either moved away from the rotor 61 or moved back to this. The pump feed fitting 101 and the Fittings 102 from the outlet feed to the column are usually held in place, except that they are rotated along with the rotation of the rotor 61 are rotated. The rotor 61 and the rotor clamp 94 When the rotor 61 is in its fluid flow position, fluid flows through it from a separate high pressure pump Isolation valve 200 to the sample injection circuit of injection valve 10 back to isolation valve 200 and then to a column. When the rotor 61 is rotated about the axis of rotation, i.e. the centerline 200CL, by means of a drive gear 205 through an angle of preferably rotated 90°, then pins 5 and 6 are repositioned to dummy fluid flow stop ports 82 and 84 which control fluid flow prevent and isolate or separate the sample loop or the sample circuit of the injection valve 10 from the rest of the HPLC system. the Those skilled in the art will recognize that the drive gear 205 is either a separate structure from the rotor clamp 94 or a one-piece structure combined with the rotor clamp 94 and even with the rotor 61. Although a drive gear shown In addition, other means known to those skilled in the art, such as a handle actuator, may be employed. Although ports 78 and 80 are preferably offset at an angle of approximately 90° from each other on the outside of the rotor are, the terminals may be aligned to abut each other. The offset is due to the advantage of threaded Connections preferred for sealing and the consequent need for larger diameter tapped holes. With Threaded tapped holes are generally 7.9 mm (5 / 16 inch) in diameter. 3A shows a top view of the housing 310 of the multi-flow isolation valves of FIGS. 1C 3B shows one Body elevational view of the multi-flow isolation valves of FIGS. 1C The isolation valve assembly 200 is disposed within the housing 310. FIG. The injection rotor 11 is within the Injection valve assembly 300 is arranged about an axis of rotation, such as centerline 300CL. The isolation valve assembly 200 and injection valve assembly 300 are preferably disposed within housing 310 by end plates 312 and 314 such that the axes of rotation, i.e. the center lines 200CL and 300CL, are parallel to each other. The valve assemblies 200 and 300 are controlled by means operated, such as a cam mechanism 320, known to those skilled in the art. Figure 3C shows a detailed view of a portion of the isolation valve assembly of Figure 3A. As above, the rotor 61 is such positioned so that the stators 202 and 204 are arranged at both ends of the rotor 61. The Belleville springs 220 and 222 steer the axial ones Loads applied to rotor 61 along centerline 200CL. The Belleville spring 220 is by means of a load washer 224 attached to one end of the stator 202. The load seal washer 224 is locked in position by a ball nut 228 . Of the Seal sheet set 206 is compressed by the axial force exerted by Belleville spring washer 232 . The ball nuts 228 and 230 (not shown) are carried by housing end plates 312 and 314, respectively, and ball nuts 228 and 230 extend through this. As described above with reference to FIG. 2A, the rotor 61 is shown in a cross-sectional view in FIG. 3C positioned between stators 202 and 204. The rotor 61 is secured by a set of three sealing layers 206 and 206 208 sealed, which are used around the pin valves 5 and 6, respectively. The preferred materials are as noted above of the gasket layers around PEEK, PTFE, PEEK, in that order. A valve end 240 of the flow-through isolation valve assembly 200 thus practically includes the stator 202 , the sealing layer 206 , the Belleville spring sealing washer 232 , the Belleville spring 220 , the load washer 224 and the ball nut 228 . Those skilled in the art will recognize that the opposite end of the valve Isolation valve assembly 200 is typically symmetrical, and therefore usually includes corresponding symmetrical components. Furthermore, those skilled in the art will recognize that the isolation valve 200 and the injection valve 300 of the combination isolation valve 10 may also be embodied by any of the alternative embodiments according to any combination of embodiments, such as these are described in a co-pending US provisional patent application referred to above. with in other words: either the first and the second embodiment or the first and the third embodiment or the second and the third Embodiment or only the second embodiment or only the third embodiment can accordingly as the isolation valve 200 and as Injection valve 300 can be used. According to a second embodiment, Figures 4A and 4B show a combination of a linear flow isolation valve 800 and a linear flow injection valve 850, the configuration of which is the substantially cylindrically shaped second embodiment of the rotor 61 resembles that in 2A and 2B. The linear flow isolation valve 800 includes a stationary element 802 and a movable one Item 804 . Movable element 804 is similar to rotor 202, except that instead of being in a to move rotational movement, by linear sliding through the stationary element 802 moves. Movable element 804 can be anything Having a cross-section, such as, but not limited to, an oval shape or a square shape with smooth, rounded corners. Movable element 804 is preferably made of either metal or a polymer or sapphire.

[0063] The stationary member 802 includes two surfaces 806a and 806b surrounding the moveable slide member 804. As shown in FIG. The two Surfaces 806a and 806b include self-energized lip seals 808a and 808b, respectively. The stationary element 802 also forms a mating surface 810 surrounding the moveable member. The pipe fitting 7 from the high-pressure pump 101 is inserted into the connection 352 of the movable element 804, where this sealed in a manner to substantially avoid external leakage. Flow is through the high pressure pump 101 to the Pin fitting 7 provided by flexible conduit 316 and connection 312. The internal line 38 within the fitting 7 is in in fluid communication with internal conduit 840 within moveable member 804 and with an open port 860 the interface or connection surface 810 . The open port 860 is in fluid communication with a chamber or a Volume of space 812 within stationary member 802 bounded by interface 810 . That Volumes of space 812 within stationary member 802 and movable member 804 are sealed by self-sustaining lip seals 808a and 808b sealed. Isolation valve pin 5 penetrates through stationary member 802 at penetration point 822 so that valve pin 5 can move linearly up and down. By means of the connection 62, the internal conduit 58 within the valve pin 5 is in fluid communication with the line casing 834 to the pin isolation valve 3 of the linear flow injection valve 850 . The line piping 66 from the mechanical seal valve 10 is then fluidly connected to the internal line 82 of the pin isolation valve 78 via the connection 70 .

[0066] Likewise, the isolation valve pin 6 penetrates the stationary member 802 at the penetration point 824 such that the Valve pin 6 can move linearly up and down. The internal line 82 within the pin isolation valve 78 is then in fluid Communicating with the volume of space 812 within the stationary element 802, which is defined by the interface or connection surface 810 is limited. The valve pin 6 is arranged to connect to the open port 880 on the mating surface 810 to become. The open port 880 is in fluid communication with the volume of space 812 . To seal the pin isolation valves 5 and 6, the stationary member 802 includes self-sustaining lip seals 820a and 820a, respectively. 820b . The lip seals are sold commercially by Furon, Inc. of Hoosick Falls, New York. During the injection phase, the internal conduit 82 within the pin isolation valve 6 is also in fluid communication with of internal conduit 876 within moveable member 804 and having an open port 874 at an opposite end of movable element 804 . Fitting 8 is inserted into open port 874 such that internal conduit 96 is within fitting 8 in Fluidic communication with a sample loop 22 is available. The internal line 96 within the fitting or the screw connection 8 is in fluid Communication with the column via flexible line 318 connected to fitting 8 by connection 314 . During the loading phase, the pin isolation valve 6 is positioned to mate with the blind port 892 on the face 810 of the movable element 804 to be connected. Likewise, the pin isolation valve 5 is arranged to mate with the blind port 888 on the surface 810 of the movable element 804 to be connected. These operations effectively isolate the flow from the high pressure pump the linear flow injection valve 850 and to the column in the same manner as described above with respect to the first embodiment has been described. A means for laterally moving the movable element 804 is provided, such as but not limited to one Linear motor 864 connected to movable element 804 and allowing pins 5 and 6 to move between the open terminals 860 and 880 or the dummy connections 888 and 892.

[0071] The linear flow injection valve 850 is analogous to the rotary injection valve 300. FIG. The linear valve 850 includes a stationary one element 802' and a moveable element 804'. The two El Elements 802' and 804' touch at a surface 810'. The movable element 804' slides along the surface 810', while the stationary Element 802' remains in position. In this embodiment, the pin isolation valve 3 having an internal conduit 58' and penetrating the Fluid flow supplied from the high pressure pump through flexible conduit 834, stationary member 802' receives at port 822'. . Pin isolation valve 3 is connected to line 834 via connection 62' and is movable within stationary member 802'. positioned so that the isolation valve pin 3 can move up and down and so that the internal conduit 58 of the valve pin 3 is in fluid communication with a first opening 860' of an internal flow line 890' passing through the moveable member 804' to the second opening 880' is guided. Both the first opening 860' and the second opening 882' are with a chamber or volume of space 812' within stationary member 802' bounded by bonding surface 810'. The stationary element 802' and movable member 804' act to seal chamber 812'. The isolation valve pin 4 is movably disposed within the stationary member 802' in the second opening 880' such that the Isolation valve pin 4 is in fluid communication with the second opening 880' of the enclosed flow channel 890'. Of the Isolation valve pin 4 having an internal conduit 96 and fluid flow from the high pressure pump 101 to the column 102 via the flexible Feeding line 836 penetrates the stationary member 802' at port 824' and is connected to the flexible line by means of a connection 854' 836 connected. The flexible line 836 is connected to the isolation valve pin 6 of the linear isolation valve 800 via a connection 70 . Flexible conduit 852' is fluidly connected to needle 12 for fluidic communication with internal conduit 812' of isolation valve pin 1 to be connected, which is movably arranged within the stationary element 802 ', so that the isolation valve pin 1 moves up and downwardly and such that the internal conduit 812' of the valve pin 1 is in fluid communication with a first port 892' an enclosed flow channel 886', which passes through the moveable element 804', via the flexible conduit 834' to convey a fluidic Establish connection to the sample loop 22. The syringe 32 is fluidly connected to the flexible conduit 856', which in turn is fluidly connected to the internal conduit 858' of the Isolation valve pin 2 is connected, which is movably arranged within the stationary element 802 ', so that the isolation valve pin 2 can move up and down and such that the internal conduit 858' of the valve pin 2 is in fluid communication with a first Opening 874' is an internal flow line 876' which is routed through the moveable member 804' to provide fluidic communication via the flexible line 836' with the sample loop 22 to produce.

[0075] The open ports 894' and 896' in the moveable member 804' serve as the ends of the internal flow line 898' such that when pin 1 and pin 2 are repositioned to connect to open ports 894' and 896', respectively, needle 12 and the syringe 32 are fluidly connected directly to each other and are separate from the sample loop 22.

[0076] The stationary member 802' includes two surfaces 806a' and 806b' surrounding the moveable slide member 804'. The two Surfaces 806a' and 806b' include self-contained lip seals 808a' and 808b', respectively. The stationary element 802' also forms a touch surface 810' surrounding the moveable element 804'.

[0077] In order to seal the isolation valve pins 1, 2, 3 and 4, the stationary member 802' includes self-sustaining lip seals 820a' and 820b', respectively. A means for laterally moving the movable element 804' is provided, such as but not limited to one Linear motor 864' connected to movable member 804' and allowing pins 1 and 2 to move between the open terminals 892' and 874' and the open ports 894' and 896', respectively. The linear motor 864' can be operated by any known means be driven, such as electrically, hydraulically or pneumatically. During the loading phase, the isolation valve pin 3 is positioned to mate with the opening 860' on the face 810' of the movable member 804' to be connected. Similarly, the isolation valve pin 4 is located, also on the face 810' of the movable element 804' with the Opening 880' to be connected. These operations effectively isolate the flow from the high pressure pump 101 to the sample loop 22 since the flow is now recirculated by pump 101 from valve pin 3 through internal line 890' to column 102 through isolation valve pin 4. The needle 12 is then fluidly connected to the sample loop 22 via the internal conduit 812' of the isolation valve pin 1, the flui dically connected to opening 894' of internal conduit 898'. Likewise, the syringe 32 becomes fluidic via the internal conduit 898' connected to sample loop 22 which is fluidly connected to port 896' of internal conduit 876'. The syringe 32 then becomes one of them used to aspirate sample fluid into the sample loop 22 from the needle 12. During the transition phase, the moveable element 804' is laterally displaced so that the isolation valve pin 1 coincides with the opening 894' and the isolation valve pin 2 is connected to the port 896' to thereby restrict flow from the needle 12 to the syringe 32 isolate. Likewise, internal conduit 58' of isolation valve pin 3 is connected to port 892' of internal conduit 886'. the internal line 96' of isolation valve pin 4 is connected to port 874' of internal line 876'.

[0082] During the injection phase, flow is increased as a result of the displacement of the movable element 804' during the transition phase from the Pump 101 through isolation valve 800 then through sample loop 22 and via isolation valve 800 onto column 102. Another variation of the second embodiment is that the stationary element 802 and the movable element 804 as to provide a duplex configuration or a mirror image configuration, such that the movable element 804 also has terminals and internal piping for the pump and column or a second pump and column to be capable of operating simultaneously as a second Mechanical seal valve to serve. Although the various embodiments of the present invention are described in terms of application to high pressure fluids have been applied, these can be applied to fluids at any working pressure, including negative pressure, i.e. vacuum applications. The invention has been described herein with reference to certain exemplary embodiments. Certain changes and modifications may be apparent to those skilled in the art without departing from the scope of the invention. The exemplary Embodiments are intended for illustrative purposes only and are not intended to limit the scope of the invention, which is defined by the appended claims is defined. SUMMARY A flow injection valve is described that includes a stationary member, a movable member, a surface of the stationary Elements touches a surface of the movable element, and has at least one pin isolation valve having an internal flow line and is movably arranged such that the internal conduit is connected to at least one flow conduit in the moveable member can be. The pin isolation valves are movably mounted so that the internal conduit is also capable of being fluidly connected to a to communicate further internal flow line in the movable element. The flow injection valve can be equipped with a similar Flow isolation valve can be connected to serve as a multiple valve and typically a conventional mechanical seal valve one Replace high pressure liquid chromatography (HPLC) systems. The multi-valve allows flow to be conveyed without the Need for switching or rotating under high pressure. Movement is by rotation or translation.

Claims

[1] flow-through injection valve, the flow-through injection valve comprising: a stationary element; a movable element, a surface of the stationary element touching a surface of the movable element; and at least one pin isolation valve; wherein the at least one pin isolation valve has an internal flow line, wherein the at least one pin isolation valve is movably arranged such that the internal conduit is adapted to be fluidly connected to at least to communicate with a flow line in the movable element, wherein the at least one pin isolation valve is movably mounted such that the internal conduit is adapted to be fluidly connected to another To communicate flow line in the movable element. [2] The flow-through injection valve of claim 1, wherein the moveable element comprises: first and second conduits for connection to internal conduits of the first and second pin isolation valves, the first and second terminating line in a surface of the movable element; a third conduit enabling fluidic communication between the internal conduits of the first and second pin isolation valves; a fourth conduit enabling fluidic communication between the internal conduits of the third and fourth pin isolation valves, wherein the third pin isolation valve provides fluid flow and the fourth pin isolation valve vents fluid flow. [3] The flow injection valve according to claim 1, wherein the movable member is moved by rotation about an axis of rotation. [4] Flow-through injection valve according to claim 1, wherein the movable element moves by a linear and / or curvilinear movement. [5] Flow injection valve according to claim 1, wherein a pin isolation valve fluidly connected to a sample loop of a High pressure liquid chromatography system (HPLC system) is connected. [6] The flow-through injection valve of claim 1, wherein a pin isolation valve is in fluid communication with a pump Promotes high pressure liquid to a high pressure liquid chromatography (HPLC) system. [7] The flow-through injection valve of claim 1, wherein a pin isolation valve is fluidly connected to a column that discharges high pressure liquid a high pressure liquid chromatography (HPLC) system. [8] Flow-through injection valve, wherein the injection valve is arranged about an axis of rotation, the injection valve comprising: at least two opposite valve ends arranged about the axis of rotation; a movable member comprising a rotor disposed between the valve ends, with an axis of rotation of the rotor parallel to the axis of rotation of the injection valve and / or coincides with it, the rotor being arranged in such a way that the orientation of the Rotor can be changed by rotating around the axis of rotation of the rotor, wherein the rotor has an exterior; at least two opposing surfaces each intersecting the outside; a first flow conduit having an opening on a first of the at least two opposing surfaces and an opening on a second of the at least two opposing surfaces; a second flow conduit having an opening on a first of the at least two opposing surfaces and an opening on one second of the at least two opposing surfaces; a flow conduit having an opening on the outside and an opening on the first of the at least two opposing surfaces having; a flow conduit having an opening on the outside and an opening on the second of the at least two opposing surfaces having; a first sealing ring for sealing the openings on the first of the at least two opposing surfaces; a second sealing ring for sealing the openings on the second of the at least two opposing surfaces; a first pin isolation valve having an internal conduit, the first pin isolation valve being arranged to be parallel to the axis of rotation of the injection valve to move, wherein the first pin isolation valve is movably arranged such that this is adapted to over the internal conduit fluidly communicating with the opening on the first flow channel on the first of the at least two opposing surfaces communicate, the first pin isolation valve being movably arranged so as to be adapted to be fluidly connected to the To communicate flow line having an opening on the outside and an opening on the second of the at least two opposite has surfaces; a second pin isolation valve having an internal conduit, the second pin isolation valve being arranged to extend parallel to the centerline of the to move the injection valve the second pin isolation valve being movably arranged to be adapted to fluidly communicate via the internal conduit with the opening on the first flow channel on the second of the at least two opposing surfaces, the second pin isolation valve being movably arranged so as to be adapted to be fluidly connected to the To communicate flow line having an opening on the outside and an opening on the second of the at least two opposite has surfaces; a third pin isolation valve having an internal conduit, the third pin isolation valve being arranged to extend parallel to the centerline of the to move the injection valve the third pin isolation valve being movably arranged to be adapted to be fluidly connected to the orifice via the internal conduit to communicate on the second flow channel on the first of the at least two opposite surfaces, the third pin isolation valve being movably arranged so as to be adapted to be fluidly connected to the To communicate flow line having an opening on the outside and an opening on the second of the at least two opposite has surfaces; and a fourth pin isolation valve having an internal conduit, the fourth pin isolation valve being arranged to extend parallel to the centerline of the to move the injection valve the fourth pin isolation valve being movably arranged to be adapted to via the internal conduit fluidly connected to the opening on the second flow channel on the second of the at least two opposite ones to communicate surfaces, the fourth pin isolation valve being movably arranged so as to be adapted to be fluidly connected to the To communicate flow line having an opening on the outside and an opening on the second of the at least two opposite has surfaces. [9] The flow-through injection valve of claim 8, wherein the rotor further comprises: a rotor clamp having an outside and an inside, the inside including at least a portion of the outside of the rotor surrounds a first opening on the outside of the rotor clamp penetrating the rotor clamp to communicate with the first opening on the outside of the rotor to collapse and a second opening on the outside of the rotor clamp penetrating the rotor clamp to communicate with the second opening on the outside of the rotor to collapse. [10] Flow injection valve according to claim 9, wherein the rotor clamp further comprises drive means for driving the rotor to the to rotate the axis of rotation of the rotor. [11] A flow-through injection valve according to claim 10, wherein said rotor clamp drive means comprises a drive gear assembly. [12] A flow-through injection valve according to claim 10, wherein the rotor clamp drive means comprises a handle actuator. [13] The flow-through injection valve of claim 8, wherein at least one of the valve ends comprises: a stator surrounding the at least one pin isolation valve, wherein the stator is adjacent to the rotor; a gasket layer surrounded within the stator and surrounding the at least one pin isolation valve to the at least one seal pin isolation valve; a Belleville spring washer; a Belleville feather; a load seal washer; and a ball nut, wherein the Belleville spring washer, the Belleville spring, the load washer and the ball nut are arranged axially about an axial Apply force to seal the gasket layer surrounding the pin isolation valve. [14] Flow injection valve according to claim 13, wherein the sealing layer made of PEEK (polyetheretherketone) and / or PTFE (polytetrafluoroethylene) consists. [15] The flow-through injection valve of claim 8, wherein the rotor is made of a PEEK blend. [16] The flow-through injection valve of claim 9, wherein the rotor clamp is made of stainless steel. [17] The flow-through injection valve of claim 16, wherein the stainless steel is Type 316 stainless steel. [18] Flow injection valve according to claim 8, wherein the first and / or the second pin isolation valve fluidly connected to a sample loop of a High pressure liquid chromatography system (HPLC system) are connected. [19] Flow injection valve according to claim 8, wherein the third and / or the fourth pin isolation valve in fluid communication with a Pump that delivers high-pressure liquid to a high-pressure liquid chromatography (HPLC) system. [20] Flow injection valve according to claim 8, wherein the third and / or the fourth pin isolation valve in fluid communication with a Column from which high pressure liquid is discharged from a high pressure liquid chromatography (HPLC) system. [21] flow injection valve comprising: a stationary element and a movable element which touch at a surface, the movable element being arranged to move along the surface to slide; a chamber located between the stationary member and the movable member, the chamber being bounded by the area, wherein the moveable member includes a first flow conduit having a first port connected to the chamber and a second opening on a surface of the movable element not connected to the chamber, wherein the moveable member includes a second flow line having a first port connected to the chamber and a second opening on a surface of the movable element not connected to the chamber, wherein the moveable member has a third passageway having a first opening and a second opening respectively on a surface of the having movable elements connected to the chamber; and wherein the moveable member has a fourth passageway having a first opening and a second opening respectively on a surface of the having a moving element associated with the chamber are connected. [22] The flow-through injection valve of claim 21, further comprising: at least (a) a first pin isolation valve, (b) a second pin isolation valve, (c) a third pin isolation valve, and / or (d) a fourth pin isolation valve; the first pin isolation valve having an internal conduit, wherein the first pin isolation valve is movably disposed within an opening within the stationary member communicating with the chamber connected such that the internal conduit is in fluid communication with the first opening on the first flow conduit on the moveable element can be wherein the first pin isolation valve is movably disposed such that the internal conduit is in fluid communication with the first port the third line can be inside the chamber, wherein the second pin isolation valve has an internal conduit, wherein the second pin isolation valve is movably disposed within an opening within the stationary member communicating with the chamber connected such that the internal conduit is in fluid communication with the first opening on the second flow conduit of the moveable elements can be wherein the second pin isolation valve is movably disposed such that the internal conduit is in fluid communication with the first port the second flow line of the movable element can be the third pin isolation valve having an internal conduit, wherein the third pin isolation valve is movably disposed within an opening within the stationary member communicating with the chamber connected so that the internal conduit can be in fluidic communication with the first opening of the fourth flow conduit, wherein the third pin isolation valve is movably arranged such that the internal conduit is in fluid communication with the first port of the first flow line can stand, wherein the fourth pin isolation valve has an internal conduit, wherein the fourth pin isolation valve is movably disposed within an opening within the stationary member communicating with the chamber connected so that the internal conduit can be in fluidic communication with the second opening of the fourth flow conduit, wherein the fourth pin isolation valve is movably arranged such that the internal conduit is in fluid communication with the second port of the second flow line can stand. [23] Flow injection valve according to claim 21, wherein the valve further comprises a housing, the stationary element and the movable element surrounds. [24] Flow injection valve according to claim 22, wherein the valve further comprises a housing, the stationary element and the movable Surrounds element and at least one of the pin isolation valves, wherein the internal conduit of the at least one pin isolation valve fluidly with connected to a line that penetrates the housing. [25] The flow-through injection valve of claim 21, wherein the valve further includes drive means for driving at least one of the moveable members includes. [26] Flow injection valve according to claim 25, wherein the drive means is a linear electric motor. [27] The flow injection valve of claim 21, wherein the first and / or the second pin isolation valve of the flow injection valve is fluidic connected to a sample loop of a high pressure liquid chromatography (HPLC) system. [28] Flow injection valve according to claim 21, wherein the third and / or the fourth pin isolation valve of the flow injection valve in in fluid communication with a pump supplying high pressure liquid to a high pressure liquid chromatography (HPLC) system encourages. [29] Flow injection valve according to claim 21, wherein the third and / or the fourth pin isolation valve of the flow injection valve in in fluid communication with a column receiving high pressure liquid from a high pressure liquid chromatography (HPLC) system system) is drained. [30] The flow injection valve of claim 23, wherein the housing of the flow injection valve is adapted to operate at pressures are greater than atmospheric pressure. [31] Flow injection valve according to claim 4, wherein the movable element consists of a PEEK (polyether ether ketone) mixture. [32] The flow injection valve of claim 4, wherein the moveable element is made of at least (a) a metal, (b) a polymer, and (c) sapphire consists. [33] Flow injection valve according to claim 4, wherein the interface between the stationary element and the movable element of the line aren injection valve is sealed by at least one lip seal. [34] The flow-through injection valve of claim 21, wherein at least one of the ports of the pin isolation valves is sealed by a lip seal is sealed. [35] The flow-through injection valve of claim 33, wherein the lip seal is self-contained. [36] The flow-through injection valve of claim 34, wherein the lip seal is self-contained. [37] multiple valve comprising: a housing; a rotary flow isolation valve disposed within the housing, the isolation valve in an axial direction for the isolation from fluid flow is arranged, the isolation valve being disposed about a centerline oriented in an axial direction, the isolation valve comprising: at least two opposite valve ends located about the centerline; a rotor disposed between the valve ends, a centerline of the rotor being substantially parallel to the centerline of the Isolation valve runs and / or coincides with this, wherein the rotor is arranged such that the orientation of the rotor by a Rotation about the centerline of the rotor can be varied, the rotor having: an outside; at least two opposing surfaces each intersecting the outside; a flow conduit having an opening on a first of the at least two opposing surfaces and an opening on a second of the has at least two opposing surfaces; a flow conduit having an opening on the outside and an opening on the first of the at least two opposing surfaces having, a flow conduit having an opening on the outside and an opening on the second of the at least two opposing surfaces having, at least one blind opening on the first of the at least two opposing surfaces, at least one blind opening on the second of the at least two opposing surfaces, a first sealing ring for sealing the openings on the first of the at least two opposing surfaces and a second sealing ring for sealing the openings on the second of the at least two opposite surfaces, a first pin isolation valve, the first pin isolation valve being arranged to move along the centerline of the isolation valve, wherein the first pin isolation valve is movably arranged to be adapted to be fluidly connected to the at least one blind port to communicate with the first of the at least two opposing surfaces, wherein the first pin isolation valve is movably arranged to be adapted to be fluidly connected to the flow line communicating, having an opening on the outside and an opening on a second of the at least two opposite surfaces having; a second pin isolation valve, the second pin isolation valve being arranged to close along the centerline of the isolation valve move; wherein the second pin isolation valve is movably arranged to be adapted to be fluidly connected to the at least one blind port to communicate on the second of the at least two opposing surfaces, wherein the second pin isolation valve is movably arranged such that it is adapted to be fluidly connected to the flow line communicating, having an opening on the outside and an opening on the second of the at least two opposite surfaces; and a rotary flow injection valve positioned within the housing to isolate fluid flow to a downstream receptacle, wherein the injection valve is arranged about a centerline oriented in an axial direction, wherein the injection valve comprises: at least two opposite valve ends located about the centerline; a rotor positioned between the valve ends, with a centerline of the rotor being parallel with the centerline of the injection valve and / or coincides therewith, the rotor being arranged such that the orientation of the rotor is determined by rotation about the centerline of the Rotors can be changed wherein the rotor has: an outside; at least two opposing surfaces each intersecting the outside; a first flow conduit having an opening on a first of the at least two opposing surfaces and an opening on a second having at least two opposing surfaces; a second flow conduit having an opening on a first of the at least two opposing surfaces and an opening on one second of the at least two opposing surfaces; a flow conduit having an opening on the outside and an opening on the first of the at least two opposing surfaces having; a flow line having an opening on the outside and an opening on the second of the we has at least two opposing surfaces; a first sealing ring for sealing the openings on the first of the at least two opposing surfaces; a second sealing ring for sealing the openings on the second of the at least two opposing surfaces; a first pin isolation valve having an internal conduit, the first pin isolation valve being arranged to extend parallel to the centerline of the injection valve to move, wherein the first pin isolation valve is movably arranged such that this is adapted to fluidly with the to communicate opening on the first flow channel on the first of the at least two opposite surfaces, wherein the first pin isolation valve is movably arranged to be adapted to be fluidly connected to the flow line communicating, having an opening on the outside and an opening on the second of the at least two opposite surfaces; a second pin isolation valve having an internal conduit, the second pin isolation valve being arranged to extend parallel to the to move the center line of the injection valve, the second pin isolation valve being movably arranged to be adapted to be fluidly connected to the opening on the first to communicate flow channel on the second of the at least two opposite surfaces, wherein the second pin isolation valve is movably arranged to be adapted to be fluidly connected to the flow line communicating, having an opening on the outside and an opening on the second of the at least two opposite surfaces; a third pin isolation valve having an internal conduit, the third pin isolation valve being arranged to extend parallel to the centerline to move the injection valve the third pin isolation valve being movably arranged to be adapted to be fluidly connected to the opening on the second to communicate flow channel on the first of the at least two opposite surfaces, wherein the third pin isolation valve is movably mounted such that it is adapted to be fluidly connected to the flow line communicating, having an opening on the outside and an opening on the second of the at least two opposite surfaces; and a fourth pin isolation valve having an internal conduit, the fourth pin isolation valve being arranged to extend parallel to the to move the center line of the injection valve, the fourth pin isolation valve being movably arranged to be adapted to be fluidly connected to the opening on the second to communicate flow channel on the second of the at least two opposite surfaces, wherein the fourth pin isolation valve is movably arranged to be adapted to be fluidly connected to the flow line communicate, having an opening on the outside and an opening on the second of the at least two opposite surfaces. [38] The multiple valve of claim 37, wherein the first pin isolation valve of the rotary flow isolation valve is fluidly connected to the third Pin isolation valve of the rotary flow injection valve is connected. [39] The multiple valve of claim 37, wherein the second pin isolation valve of the rotary flow isolation valve is fluidly connected to the fourth Pin isolation valve of the rotary flow injection valve is connected. [40] The multiple valve of claim 37, wherein one of said rotors further comprises: a rotor clamp having an outside and an inside, the inside including at least a portion of the outside of the rotor surrounds a first opening on the outside of the rotor clamp penetrating the rotor clamp to communicate with the first opening on the outside of the rotor to collapse, and a second opening on the outside of the rotor clamp penetrating the rotor clamp to communicate with the second opening on the outside of the rotor to collapse. [41] The multiple valve of claim 40, wherein the rotary flow isolation valve further comprises at least one of (a) a third pin isolation valve and (b) a fourth pin isolation valve comprises: wherein the third pin isolation valve has an internal conduit, the third pin isolation valve being within the first opening on the Outside of the rotor clamp is arranged so that the internal line of the third pin isolation valve is arranged to be in fluid To be in communication with the opening on the outside of the flow line, which is an opening on the outside and an opening on the first having at least two surfaces intersecting the outside of the rotor, wherein the fourth pin isolation valve has an internal conduit, the fourth pin isolation valve being within the second opening the outside of the rotor clamp is arranged so that the internal line of the fourth pin isolation valve is arranged to be in fluid To be in communication with the orifice on the outside of the flow line, which is an orifice on the outside and an orifice on the second of the at least two surfaces intersecting the outside of the rotor. [42] The multiple valve of claim 40, wherein the rotor clamp further comprises drive means for driving the rotor to rotate about the centerline of the to rotate the rotors. [43] The multiple valve of claim 42, wherein the rotor clamp drive means comprises a drive gear actuator. [44] The multiple valve of claim 42 wherein the rotor clamp drive means comprises a handle actuator. [45] The multiple valve of claim 37, wherein at least one of the valve ends comprises: a stator surrounding the at least one pin isolation valve, the stator being adjacent to the rotor; a gasket layer enclosed within the stator and enclosing the at least one pin isolation valve to the at least one seal pin isolation valve; a Belleville spring washer; a Belleville feather; a load seal washer; and a ball nut, wherein the Belleville spring washer, the Belleville spring, the load washer and the ball nut are arranged axially about an axial Apply force to seal the gasket layer enclosing the pin isolation valve. [46] Multiple valve according to claim 45, wherein the sealing layer consists of at least PEEK (polyetheretherketone) and / or PTFE (polytetrafluoroethylene) consists. [47] The multiple valve of claim 37, wherein at least one of the rotors is made of a PEEK blend. [48] ​​The multiple valve of claim 40, wherein the rotor clamp is made of a stainless steel. [49] The multiple valve of claim 48, wherein the stainless steel is a type 316 stainless steel. [50] The multiple valve of claim 37, wherein the first and / or second pin isolation valve of the rotary flow injection valve is fluidly connected a sample loop of a high pressure liquid chromatography (HPLC) system. [51] The multiple valve of claim 41 wherein the third and / or fourth pin isolation valve of the rotary flow isolation valve is fluidic connected to a pump that delivers high pressure liquid to a high pressure liquid chromatography (HPLC) system. [52] The multiple valve of claim 41, wherein the third and / or fourth pin isolation valve of the rotary flow isolation valve is fluidic connected to a column from which high pressure liquid is discharged from a high pressure liquid chromatography (HPLC) system becomes. [53] multiple valve comprising: a housing; a rotary flow isolation valve disposed within the housing, the isolation valve in an axial direction for the isolation from fluid flow is arranged, wherein the isolation valve is arranged about an axis of rotation, wherein the isolation valve comprises: at least two opposite valve ends arranged about the axis of rotation; a rotor disposed between the valve ends, wherein an axis of rotation of the rotor is substantially parallel to the axis of rotation of the isolation valve runs and / or coincides with this, wherein the rotor is arranged such that the orientation of the rotor by a Rotation about the axis of rotation of the rotor can be varied, the rotor having: an outside; at least two opposing surfaces each intersecting the outside; a flow conduit having an opening on a first of the at least two opposing surfaces and an opening on a second of the has at least two opposing surfaces; a flow conduit having an opening on the outside and an opening on the first of the at least two opposing surfaces having, a flow conduit having an opening on the outside and an opening on the second of the at least two opposing surfaces having, at least one blind opening on the first of the at least two opposing surfaces, at least one blind opening on the second of the at least two opposing surfaces, a first sealing ring for sealing the openings on the first of the at least two opposing surfaces and a second sealing ring for sealing the openings on the second of the at least two opposite surfaces, a first pin isolation valve, the first pin isolation valve being arranged to move along the centerline of the isolation valve, wherein the first pin isolation valve is movably arranged to be adapted to be fluidly connected to the at least one blind port to communicate with the first of the at least two opposing surfaces, wherein the first pin isolation valve is movably arranged to be adapted to be fluidly connected to the flow line communicating, having an opening on the outside and an opening on a second of the at least two opposite surfaces having; a second pin isolation valve, the second pin isolation valve being arranged to close along the centerline of the isolation valve move; wherein the second pin isolation valve is movably arranged to be adapted to be fluidly connected to the at least one blind port to communicate on the second of the at least two opposing surfaces, the second pin isolation valve being movably arranged to be adapted to fluidly communicate with the flow line having an opening on the outside and an opening on the second of the at least two opposing surfaces; and a linear flow injection valve, the injection valve comprising: a stationary element; a movable element; wherein the stationary element and the movable element touch at a surface with the movable element being arranged to along to slide the surface; a chamber disposed between the stationary member and the movable member, the chamber being defined by the area; wherein the moveable member includes a first flow conduit having a first port connected to the chamber and a opening on a surface of the movable element not connected to the chamber, wherein the moveable member includes a second flow line having a first port connected to the chamber and a second opening on a surface of the movable element not connected to the chamber, wherein the moveable member has a third flow passage having first and second openings respectively on a surface of the having movable elements connected to the chamber, wherein the moveable member has a fourth passageway having first and second openings respectively on a surface of the having movable elements connected to the chamber, and a second blind opening on the surface delimiting the chamber. [54] The multiple valve of claim 53, wherein the linear flow injection valve further comprises: at least (a) a first pin isolation valve, (b) a second pin isolation valve, (c) a third pin isolation valve, and / or (d) a fourth pin isolation valve; the first pin isolation valve having an internal conduit, wherein the first pin isolation valve is located within an opening within the stationary member that is connected to the chamber, so that the internal conduit of the first pin isolation valve is movably arranged to be in fluid communication with the first port on a to stand first flow line of the moving element, wherein the internal conduit of the first pin isolation valve is movably arranged to be in fluid communication with the first port on the to stand third flow line, wherein the second pin isolation valve has an internal conduit, wherein the second pin isolation valve is located within an opening within the stationary member that is connected to the chamber, so that the internal conduit of the second pin isolation valve is movably arranged to be in fluid communication with the first port on a to stand second flow line of the moving element, wherein the internal conduit of the second pin isolation valve is movably arranged to be in fluid communication with the second port of the to stand third flow line, the third pin isolation valve having an internal conduit, wherein the third pin isolation valve is located within an opening within the stationary member that is connected to the chamber, so that the internal conduit of the third pin isolation valve is movably arranged to be in fluid communication with the first port of the fourth to stand flow line, wherein the internal conduit of the third pin isolation valve is movably arranged to be in fluid communication with the first port of the first to stand flow line, wherein the fourth pin isolation valve has an internal conduit, wherein the fourth pin isolation valve is located within an opening within the stationary member which is connected to the chamber, such that the internal conduit of the fourth pin isolation valve is movably arranged to be in fluid communication with the second port of the fourth flow line to stand, wherein the internal conduit of the fourth pin isolation valve is movably arranged to be in fluid communication with the first port on the second flow line to stand. [55] The multiple valve of claim 53, wherein the linear flow injection valve further comprises a housing that houses the stationary element and the moving element surrounds. [56] Multiple valve according to claim 54, wherein the linear flow injection valve further comprises a housing which the stationary element and surrounding the moveable member and at least one of the pin isolation valves, the internal conduit of the at least one pin isolation valve is fluidly connected to a line which penetrates the housing. [57] The multiple valve of claim 53, wherein the linear injection valve further comprises drive means for moving the movable member. [58] Multiple valve according to claim 57; wherein the drive means is a linear motor. [59] Multiple valve according to claim 54, wherein the first and / or the second pin isolation valve of the linear injection valve fluidly with a Sample loop of a high pressure liquid chromatography (HPLC) system. [60] Multiple valve according to claim 54, wherein the third and / or the fourth pin isolation valve of the rotary isolation valve fluidly with a Pump are connected, the high pressure liquid to a high pressure liquid chromatography system (HPLC system) promotes. [61] Multiple valve according to claim 54, wherein the third and / or the fourth pin isolation valve of the rotary isolation valve fluidly with a Column that discharges high pressure liquid from a high pressure liquid chromatography (HPLC) system. [62] The multiple valve of claim 55, wherein the housing of the linear injection valve is adapted to be operated at pressures that are greater than atmospheric pressure. [63] The multiple valve of claim 56, wherein the housing of the linear injection valve is adapted to be operated at pressures that are greater than atmospheric pressure. [64] The multiple valve of claim 53, wherein the moveable member is made of a PEEK (polyetheretherketone) blend. [65] The multiple valve of claim 53, wherein the first pin isolation valve of the flow-through isolation valve is fluidly connected to the third Pin isolation valve of the flow injection valve is connected. [66] The multiple valve of claim 53, wherein the second pin isolation valve of the rotary flow isolation valve is fluidly connected to the fourth Pin isolation valve of the rotary flow injection valve is connected. [67] The multiple valve of claim 53, wherein the rotor further comprises: a rotor clamp having an outside and an inside, the inside including at least a portion of the outside of the rotor surrounds a first opening on the outside of the rotor clamp penetrating through the rotor clamp to coincide with the first opening on the outside of the rotor and a second opening on the outside of the rotor clamp penetrating the rotor clamp to communicate with the second opening on the outside of the rotor to collapse. [68] The multiple valve of claim 67, wherein the rotary flow isolation valve further comprises at least one of (a) a third pin isolation valve and (b) includes a fourth pin isolation valve; wherein the third pin isolation valve has an internal conduit, the third pin isolation valve being within the first opening on the Outside of the rotor clamp is arranged so that the internal line of the third pin isolation valve is arranged to be in fluid To stand in communication with the opening on the outside of the flow line, which has an opening on the outside and an opening on the first of the at least two surfaces intersecting the outside of the rotor, wherein the fourth pin isolation valve has an internal conduit, the fourth pin isolation valve being within the second opening the outside of the rotor clamp is arranged so that the internal line of the fourth pin isolation valve is arranged to be in fluid To stand in communication with the opening on the outside of the flow line, which has an opening on the outside and an opening on the second of the at least two surfaces intersecting the outside of the rotor. [69] The multiple valve of claim 68 wherein the third and / or fourth pin isolation valve of the rotary flow isolation valve is within one of the openings are located on the outside of the rotor clamp by means of a threaded compression connection. [70] The multiple valve of claim 67, wherein the rotor clamp further comprises drive means for driving the rotor to rotate about the axis of rotation of the rotor to rotate. [71] The multiple valve of claim 70, wherein the rotor clamp drive means comprises a drive gear actuator. [72] The multiple valve of claim 70, wherein the rotor clamp drive means comprises a handle actuator. [73] Multiple valve according to claim 53, wherein the interface between the stationary element and the movable element of the linear Injection valve is sealed by at least one lip seal. [74] Multiple valve according to claim 54, wherein at least one of the openings of the pin isolation valves of the linear injection valve by a Lip seal is sealed. [75] The multiple valve of claim 73, wherein the lip seal is self-contained. [76] The multiple valve of claim 74, wherein the lip seal is self-contained. [77] The multiple valve of claim 53, wherein the moveable element consists of at least (a) a metal, (b) a polymer, and (c) sapphire. [78] The multiple valve of claim 53, wherein at least one of the valve ends comprises: a stator enclosing the at least one pin isolation valve, the stator being adjacent to the rotor; a gasket layer enclosed within the stator and enclosing the at least one pin isolation valve to the at least one seal pin isolation valve; a Belleville spring washer; a Belleville feather; a load seal washer; and a ball nut, wherein the Belleville spring washer, the Belleville spring, the load washer and the ball nut are arranged axially about an axial Apply force to seal the gasket layer enclosing the pin isolation valve. [79] Multiple valve according to claim 78, wherein the sealing layer consists of at least PEEK (polyetheretherketone) and / or PTFE (polytetrafluoroethylene) consists. [80] The multiple valve of claim 53, wherein the rotor is made of a PEEK blend. [81] The multiple valve of claim 67, wherein the rotor clamp is made of stainless steel. [82] The multiple valve of claim 81, wherein the stainless steel is ASTM Type 316 stainless steel. [83] Multiple valve comprising: a housing; a linear flow isolation valve disposed within the housing, the isolation valve comprising: a stationary element; a movable element; wherein the stationary element and the movable element touch at a surface with the movable element being arranged to along to slide the surface; a chamber disposed between the stationary member and the movable member, the chamber being defined by the area; wherein the moveable member includes a first flow line having an orifice connected to the chamber and an orifice on a surface of the movable element not connected to the chamber, wherein the moveable member includes a second flow line having an orifice connected to the chamber and a opening on a surface of the movable element not connected to the chamber, a first blind opening on the surface delimiting the chamber, a second blind opening on the surface delimiting the chamber, a rotary flow injection valve positioned within the housing to isolate flow to a downstream receptacle, wherein the injection valve is arranged about an axis of rotation oriented in the injection valve, comprising: at least two opposite valve ends located about the centerline; a rotor disposed between the valve ends, a centerline of the rotor being substantially parallel to the centerline of the Injection valve runs and / or coincides with this, wherein the rotor is arranged such that the orientation of the rotor by a Rotation about the centerline of the rotor can be changed, the rotor having: an outside; at least two opposing surfaces each intersecting the outside; a first flow conduit having an opening on a first of the at least two opposing surfaces and an opening on a second of the at least two opposing surfaces; a second flow conduit having an opening on a first of the at least two opposing surfaces and an opening on one second of the at least two opposing surfaces; a flow conduit having an opening on the outside and an opening on the first of the at least two opposing surfaces having, a flow conduit having an opening on the outside and an opening on the second of the at least two opposing surfaces, a first sealing ring for sealing the opening genes on the first of the at least two opposing surfaces and a second sealing ring for sealing the openings on the second of the at least two opposite surfaces, a first pin isolation valve, the first pin isolation valve being arranged to parallel the centerline of the injection valve move, the first pin isolation valve being movably arranged to be adapted to be fluidly connected to the opening on the first to communicate flow line on the first of the at least two opposing surfaces, wherein the first pin isolation valve is movably arranged to be adapted to be fluidly connected to the flow line communicating, having an opening on the outside and an opening on the second of the at least two opposite surfaces; a second pin isolation valve, the second pin isolation valve being arranged to parallel the centerline of the injection valve move; the second pin isolation valve being movably arranged so as to be adapted to be fluidly connected to the opening on the first to communicate flow line on the second of the at least two opposing surfaces, wherein the second pin isolation valve is movably arranged such that it is adapted to be fluidly connected to the flow line communicating, having an opening on the outside and an opening on the second of the at least two opposite surfaces; a third pin isolation valve, the third pin isolation valve being arranged to move parallel to the centerline of the injection valve, the third pin isolation valve being movably arranged to be adapted to be fluidly connected to the opening on the second to communicate flow line on the first of the at least two opposing surfaces, wherein the third pin isolation valve is movably arranged to be adapted to be fluidly connected to the flow line communicating, having an opening on the outside and an opening on the second of the at least two opposite surfaces; a fourth pin isolation valve, the fourth pin isolation valve being arranged to parallel the centerline of the injection valve move; the fourth pin isolation valve being movably arranged so as to be adapted to be fluidly connected to the opening on the second to communicate flow line on the second of the at least two opposing surfaces, wherein the fourth pin isolation valve is movably arranged such that it is adapted to be fluidly connected to the flow line communicate, having an opening on the outside and an opening on the second of the at least two opposite surfaces. [84] The multiple valve of claim 83, wherein the linear flow isolation valve further comprises: at least (a) a first pin isolation valve, (b) a second pin isolation valve, (c) a third pin isolation valve, and / or (d) a fourth pin isolation valve; the first pin isolation valve having an internal conduit, wherein the first pin isolation valve is located within an opening within the stationary member that is connected to the chamber, so that the internal conduit of the first pin isolation valve is movably arranged to be in fluid communication with the first port on a to stand first flow line of the moving element, wherein the internal conduit of the first pin isolation valve is movably arranged to be in fluid communication with the first blind port to stand the surface delimiting the chamber, wherein the second pin isolation valve has an internal conduit, wherein the second pin isolation valve is located within an opening within the stationary member that is connected to the chamber, so that the internal conduit of the second pin isolation valve is movably arranged to be in fluid communication with the first port on a to stand second flow line of the moving element, wherein the internal conduit of the second pin isolation valve is movably disposed to be in fluid communication with the second blind port to stand the surface delimiting the chamber, the third pin isolation valve having an internal conduit, wherein the third pin isolation valve is located within an opening within the stationary member that is connected to the chamber, so that the internal conduit of the third pin isolation valve is movably arranged to be in fluidic communication with the first port of the third to stand flow line, wherein the internal conduit of the third pin isolation valve is movably disposed to be in fluid communication with the first blind port to stand the surface delimiting the chamber, wherein the fourth pin isolation valve has an internal conduit, wherein the fourth pin isolation valve is located within an opening within the stationary member connected to the chamber, so that the internal conduit of the fourth pin isolation valve is movably arranged to be in fluidic communication with the second port of the third to stand flow line, wherein the internal conduit of the fourth pin isolation valve is movably disposed to fluidly To stand in communication with the second blind opening on the surface delimiting the chamber. [85] Multiple valve according to claim 83, wherein the linear flow isolation valve further comprises a housing that the stationary element and surrounds the movable element. [86] Multiple valve according to claim 84, wherein the linear flow isolation valve further comprises a housing, the stationary member and surrounding the moveable member and at least one of the pin isolation valves, the internal conduit of the at least one pin isolation valve is fluidly connected to a line which penetrates the housing. [87] The multiple valve of claim 83, wherein the linear isolation valve further comprises drive means for moving the moveable member. [88] The multiple valve of claim 87, wherein the drive means is a linear motor. [89] Multiple valve according to claim 84, wherein the first and / or the second pin isolation valve of the rotary injection valve fluidly with a Sample loop of a high pressure liquid chromatography (HPLC) system. [90] Multiple valve according to claim 84, wherein the third and / or the fourth pin isolation valve of the linear isolation valve fluidly with a Pump are connected, the high pressure liquid to a high pressure liquid chromatography system (HPLC system) promotes. [91] The multiple valve of claim 84 wherein the third and / or fourth pin isolation valve of the linear isolation valve is fluidly connected to a Column that discharges high pressure liquid from a high pressure liquid chromatography (HPLC) system. [92] The multiple valve of claim 85, wherein the housing of the linear isolation valve is adapted to be operated at pressures that are greater than atmospheric pressure. [93] The multiple valve of claim 86, wherein the housing of the linear isolation valve is adapted to be operated at pressures that are greater than atmospheric pressure. [94] The multiple valve of claim 83, wherein the moveable member is made of a PEEK (polyetheretherketone) blend. [95] The multiple valve of claim 83, wherein the first pin isolation valve of the linear isolation valve is fluidly connected to the third Pin isolation valve of the rotary injection valve is connected. [96] The multiple valve of claim 83, wherein the second pin isolation valve of the linear isolation valve is fluidly connected to the fourth Pin isolation valve of the rotary injection valve is connected. [97] The multiple valve of claim 83, wherein the rotor further comprises: a rotor clamp having an outside and an inside, the inside including at least a portion of the outside of the rotor surrounds a first opening on the outside of the rotor clamp penetrating the rotor clamp to communicate with the first opening on the outside of the rotor to collapse and a second opening on the outside of the rotor clamp penetrating the rotor clamp to communicate with the second opening on the outside of the rotor to collapse. [98] The multiple valve of claim 97, wherein the rotary flow injection valve further includes at least one of (a) a third pin isolation valve and (b) includes a fourth pin isolation valve; wherein the third pin isolation valve has an internal conduit, the third pin isolation valve being within the first opening on the Outside of the rotor clamp is arranged so that the internal line of the third pin isolation valve is arranged to be in fluid To be in communication with the opening on the outside of the flow line, which is an opening on the outside and an opening on the first having at least two surfaces intersecting the outside of the rotor, wherein the fourth pin isolation valve has an internal conduit, the fourth pin isolation valve being within the second opening the outside of the rotor clamp is arranged so that the internal line of the fourth pin isolation valve is arranged to be in fluid To be in communication with the orifice on the outside of the flow line, which is an orifice on the outside and an orifice on the second of the at least two surfaces intersecting the outside of the rotor. [99] The multiple valve of claim 98 wherein the third and / or fourth pin isolation valve is within one of the ports on the outside of the rotor clamp by means of a threaded compression connection. [100] The multiple valve of claim 97, wherein the rotor clamp further comprises drive means for driving the rotor to rotate about the axis of rotation of the rotor to rotate. [101] The multiple valve of claim 100, wherein the rotor clamp drive means comprises a drive gear actuator. [102] The multiple valve of claim 100 wherein the rotor clamp drive means comprises a handle actuator. [103] The multiple valve of claim 83, wherein at least one of the valve ends comprises: a stator surrounding the at least one pin isolation valve, the stator being adjacent to the rotor; a gasket layer enclosed within the stator and enclosing the at least one pin isolation valve to the at least one seal pin isolation valve; a Belleville spring washer; a Belleville feather; a load seal washer; and a ball nut, wherein the Belleville spring washer, the Belleville spring, the load washer and the ball nut are arranged axially about an axial Apply force to seal the gasket layer enclosing the pin isolation valve. [104] Multiple valve according to claim 103, wherein the sealing layer consists of at least PEEK (polyetheretherketone) and / or PTFE (polytetrafluoroethylene). [105] The multiple valve of claim 83, wherein the rotor is made of a PEEK blend. [106] The multiple valve of claim 97, wherein the rotor clamp is made of a stainless steel. [107] The multiple valve of claim 106, wherein the stainless steel is ASTM Type 316 stainless steel. [108] Multiple valve according to claim 83, wherein the interface between the stationary element and the movable element of the linear Isolation valve is sealed by at least one lip seal. [109] Multiple valve according to claim 84, wherein at least one of the openings of the pin isolation valves of the linear isolation valve by a Lip seal is sealed. [110] The multiple valve of claim 108, wherein the lip seal is self-contained. [111] The multiple valve of claim 109, wherein the lip seal is self-contained. [112] The multiple valve of claim 56, wherein the moveable element consists of at least (a) a metal, (b) a polymer, and (c) sapphire. [113] multiple valve comprising: a housing; a linear flow isolation valve disposed within the housing, the isolation valve comprising: a stationary element; a movable element; wherein the stationary element and the movable element touch at a surface with the movable element being arranged to along to slide the surface; a chamber disposed between the stationary member and the movable member, the chamber being defined by the area; wherein the moveable member includes a first flow line having an orifice connected to the chamber and an orifice on a surface of the movable element not connected to the chamber, wherein the moveable member includes a second flow line having an orifice connected to the chamber and a opening on a surface of the movable element not connected to the chamber, a first blind opening on the surface delimiting the chamber, a second blind opening on the surface delimiting the chamber, a linear flow injection valve, the injection valve comprising: a stationary element; a movable element;wherein the stationary element and the movable element touch at a surface, the movable element being arranged to be along to slide the surface; a chamber disposed between the stationary member and the movable member, the chamber being defined by the area; wherein the moveable member includes a first flow conduit having a first port connected to the chamber and a second opening on a surface of the movable element not connected to the chamber, wherein the moveable member includes a second flow line having a first port connected to the chamber and a second opening on a surface of the movable element not connected to the chamber, wherein the moveable member has a third passageway having a first opening and a second opening respectively on a surface of the having movable elements connected to the chamber, wherein the moveable member has a fourth passageway having a first opening and a second opening respectively on a surface of the be movable elements which are connected to the chamber. [114] The multiple valve of claim 113, wherein the flow-through injection valve further comprises: at least (a) a first pin isolation valve, (b) a second pin isolation valve, (c) a third pin isolation valve, and / or (d) a fourth pin isolation valve; the first pin isolation valve having an internal conduit, wherein the first pin isolation valve is located within an opening within the stationary member that is connected to the chamber, so that the internal conduit of the first pin isolation valve is movably arranged to be in fluid communication with the first port on a to stand first flow line of the moving element, wherein the internal conduit of the first pin isolation valve is movably arranged to be in fluid communication with the first port of the third to stand flow line, wherein the second pin isolation valve has an internal conduit, wherein the second pin isolation valve is located within an opening within the stationary member that is connected to the chamber, so that the internal conduit of the second pin isolation valve is movably arranged to be in fluid communication with the first port on a to stand second flow line of the moving element, wherein the internal conduit of the second pin isolation valve is movably arranged to be in fluid communication with the second port of the to stand third flow line, the third pin isolation valve having an internal conduit, wherein the third pin isolation valve is located within an opening within the stationary member that is connected to the chamber, so that the internal conduit of the third pin isolation valve is movably arranged to be in fluid communication with the first port of the fourth to stand flow line, wherein the internal conduit of the third pin isolation valve is movably arranged to be in fluid communication with the first port of the first to stand flow line, wherein the fourth pin isolation valve has an internal conduit, wherein the fourth pin isolation valve is located within an opening within the stationary member which is connected to the chamber, such that the internal conduit of the fourth pin isolation valve is movably arranged to be in fluid communication with the second port of the fourth flow line to stand, wherein the internal conduit of the fourth pin isolation valve is movably arranged to be in fluid communication with the first port of the second flow line to stand. [115] The multiple valve of claim 113, wherein at least one of the flow isolation valve and the flow injection valve further includes a Include housing surrounding the stationary member and the movable member. [116] The multiple valve of claim 114, wherein at least one of the flow isolation valve and the flow injection valve further comprises a Include housing surrounding the stationary member and the movable member and at least one of the pin isolation valves, wherein the internal Line of at least one pin isolation valve is fluidly connected to a line that penetrates the housing. [117] The multiple valve of claim 113, further comprising driving means for driving at least one of the movable members. [118] The multiple valve of claim 117, wherein the drive means is a linear electric motor. [119] Multiple valve according to claim 113, wherein the first and / or the second pin isolation valve of the flow injection valve fluidly with a Sample loop of a high pressure liquid chromatography (HPLC) system. [120] The multiple valve of claim 113 wherein the third and / or fourth pin isolation valve of the flow-through isolation valve is fluidically connected to a Pump are connected, the high pressure liquid to a high pressure liquid chromatography system (HPLC system) promotes. [121] The multiple valve of claim 113 wherein the third and / or fourth pin isolation valve of the flow-through isolation valve is fluidically connected to a Column that discharges high pressure liquid from a high pressure liquid chromatography (HPLC) system. [122] Multiple valve according to claim 115, wherein the housing of the flow isolation valve and / or the flow injection valve thereto is capable of operating at pressures greater than atmospheric pressure. [123] Multiple valve according to claim 116, wherein the housing of the flow isolation valve and / or the flow injection valve thereto is capable of operating at pressures greater than atmospheric pressure. [124] Multiple valve according to claim 113, wherein at least one of the movable elements made of a PEEK mixture (polyetheretherketone mixture) exists. [125] The multiple valve of claim 113, wherein the interface between the stationary member and the movable member at least of the linear injection valve and / or the linear isolation valve is sealed by at least one lip seal. [126] Multiple valve according to claim 114, wherein at least one of the openings of the pin isolation valves of the linear valves by a Lip seal is sealed. [127] The multiple valve of claim 125, wherein the lip seal is self-contained. [128] The multiple valve of claim 126, wherein the lip seal is self-contained. [129] The multiple valve of claim 113, wherein the moveable element consists of at least (a) a metal, (b) a polymer, and (c) sapphire. [130] A method of operating a flow-through injection valve, the valve comprising: a moveable element, the moveable element having a first and a second conduit for connection to internal conduits of the first and the second pin isolation valve, the first and second conduits terminating in a surface of the moveable member; a third conduit enabling fluidic communication between the internal conduits of the first and second pin isolation valves; a fourth Conduit enabling fluidic communication between internal conduits of the third and fourth pin isolation valves, the third pin isolation valve provides fluid flow and the fourth pin isolation valve vents fluid flow; (A) wherein the valve is in an initial flow isolation position such that the third pin isolation valve providing fluid flow, is in fluidic communication with the fourth pin isolation valve venting fluid flow, the first pin isolation valve in fluidic is in communication with the first line and the second pin isolation valve is in fluid communication with the second line; the method comprising the following steps: (I) wherein the first pin isolation valve is connected to the first line, (1) moving the first pin isolation valve away from the first conduit; (2) moving the movable element, (3) Moving the first pin isolation valve toward the movable member so that the internal conduit within the first pin isolation valve is connected to the third line; and (II) wherein the second pin isolation valve is connected to the second line, (1) moving the second pin isolation valve away from the second conduit; (2) moving the movable element, (3) Moving the second pin isolation valve toward the movable member so that the internal conduit within the second Pin isolation valve is connected to the third line to thereby fluidic communication between the first and the second to manufacture pin isolation valve; and (III) wherein the third pin isolation valve is connected to the fourth line, (1) moving the third pin isolation valve away from the fourth line, (2) moving the movable element; (3) moving the third pin isolation valve toward the first conduit to be in fluid communication with the internal conduit of the third manufacture pin isolation valve; and (IV) wherein the fourth pin isolation valve is connected to the fourth line, (1) moving the fourth pin isolation valve away from the fourth conduit; (2) moving the movable element; (3) moving the fourth pin isolation valve toward the second line to be in fluid communication with the internal line of the fourth manufacture pin isolation valve; and (B) the valve being in an initial flow passage position such that at least (a) the third pin isolation valve restricts fluid flow provides, is connected to the first line and / or (b) the fourth pin isolation valve, which bleeds fluid flow, to the second line connected is, the method comprising the steps of: (III) wherein the third pin isolation valve is connected to the first line, (1) moving the third pin isolation valve away from the first line, (2) moving the movable element and (3) Moving the third pin isolation valve toward the movable member so that the internal line within the third pin isolation valve is connected to the fourth line; and (IV) wherein the fourth pin isolation valve is connected to the second line, (1) moving the fourth pin isolation valve away from the second line, (2) moving the movable element and (3) Moving the fourth pin isolation valve toward the movable member so that the internal conduit within the second pin isolation valve is connected to the first line; (V) wherein the first pin isolation valve is connected to the third line, (1) Move the first pin isolation valve away from the third line, (2) moving the movable element and (3) Moving the first pin isolation valve toward the movable member so that the internal conduit within the first pin isolation valve is connected to the first line; and (VI) wherein the second pin isolation valve is connected to the third line, (1) moving the second pin isolation valve away from the third line, (2) moving the movable element and (3) Moving the second pin isolation valve toward the movable member so that the internal conduit within the second Pin isolation valve is connected to the second line. [131] A method of operating a multi-valve, the multi-valve comprising a flow-through isolation valve, wherein the Flow Isolation Valve Includes: a moveable element, the moveable element having a first and a second conduit for connection to internal conduits of a first and a second pin isolation valve, the conduits terminating in a surface of the moveable member; first and second blind ports for connection to the internal lines of the first and second pin isolation valve, (A) wherein the valve is in an initial flow isolation position such that at least (a) the first pin isolation valve restricts fluid flow provides, is connected to the first blind port and / or (b) the second pin isolation valve, which bleeds fluid flow, to the second blind opening is connected, the method comprising the steps of: (I) wherein the first pin isolation valve is connected to the first blind port, (1) moving the first pin isolation valve away from the first blind hole, (2) moving the movable element and (3) Moving the first pin isolation valve toward the movable member so that the internal conduit within the first pin isolation valve is connected to the first line terminating in a surface of the movable member; and (II) wherein the second pin isolation valve is connected to the second blind port, (1) moving the second pin isolation valve away from the second blind port, (2) moving the movable element and (3) moving the second pin isolation valve toward the moveable member such that the internal conduit within the second pin isolation valve is connected to the second conduit terminating in a surface of the moveable member, and (B) wherein the valve is in an initial flow passage position such that at least (a) the first pin isolation valve restricts fluid flow is connected to the first line and / or (b) the second pin isolation valve, which bleeds fluid flow, to the second line connected is, the method comprising the steps of: (III) wherein the first pin isolation valve is connected to the first line, (1) moving the first pin isolation valve away from the first line, (2) moving the movable element and (3) Moving the first pin isolation valve toward the movable member so that the internal conduit within the first pin isolation valve is connected to the first blind port; and (IV) wherein the second pin isolation valve is connected to the second line, (1) moving the second pin isolation valve away from the second line, (2) moving the movable element and (3) Moving the second pin isolation valve toward the movable member so that the internal conduit within the second pin isolation valve is connected to the second blind port, and wherein the multiple valve comprises a flow-through injection valve, the flow-through injection valve comprising: a moveable element, the moveable element having a first and a second conduit for connection to internal conduits of the first and the second pin isolation valve, the first and second conduits terminating in a surface of the moveable member; a third conduit enabling fluidic communication between the internal conduits of the first and second pin isolation valves; a fourth Conduit enabling fluidic communication between the internal conduits of the third and fourth pin isolation valves, wherein the third pin isolation valve provides fluid flow, wherein the fourth pin isolation valve vents fluid flow; (A) wherein the valve is in an initial flow isolation position such that the third pin isolation valve providing fluid flow is in is in fluid communication with the fourth pin isolation valve venting fluid flow, the first pin isolation valve in fluid is in communication with the first line and the second pin isolation valve is in fluid communication with the second line; the method comprising the following steps: (I) wherein the first pin isolation valve is connected to the first line, (1) moving the first pin isolation valve away from the first conduit; (2) moving the movable element, (3) Moving the first pin isolation valve toward the movable member so that the internal conduit within the first pin isolation valve is connected to the third line; and (II) wherein the second pin isolation valve is connected to the second line, (1) moving the second pin isolation valve away from the second conduit; (2) moving the movable element, (3) Moving the second pin isolation valve toward the movable member so that the internal conduit within the second Pin isolation valve is connected to the third line to thereby fluidic communication between the first and the second manufacture pin isolation valve; and (III) wherein the third pin isolation valve is connected to the fourth line, (1) moving the third pin isolation valve away from the fourth line, (2) moving the movable element; (3) moving the third pin isolation valve toward the first conduit to be in fluid communication with the internal conduit of the third manufacture pin isolation valve; and (IV) wherein the fourth pin isolation valve is connected to the fourth line, (1) moving the fourth pin isolation valve away from the fourth conduit; (2) moving the movable element; (3) moving the fourth pin isolation valve toward the second line to be in fluid communication with the internal line of the fourth manufacture pin isolation valve; and (B) the valve being in an initial flow passage position such that at least (a) the third pin isolation valve restricts fluid flow provides, is connected to the first line and / or (b) the fourth pin isolation valve, which bleeds fluid flow, to the second line connected is, the method comprising the steps of: (III) wherein the third pin isolation valve is connected to the first line, (1) moving the third pin isolation valve away from the first line, (2) moving the movable element and (3) Moving the third pin isolation valve toward the movable member so that the internal line within the third pin isolation valve is connected to the fourth line; and (IV) wherein the fourth pin isolation valve is connected to the second line, (1) moving the fourth pin isolation valve away from the second line, (2) moving the movable element and (3) Moving the fourth pin isolation valve toward the movable member so that the internal conduit within the second pin isolation valve is connected to the first line; and (V) wherein the first pin isolation valve is connected to the third line, (1) moving the first pin isolation valve away from the third line, (2) moving the movable element and (3) Moving the first pin isolation valve toward the movable member so that the internal conduit within the first pin isolation valve is connected to the first line; and (VI) wherein the second pin isolation valve is connected to the third line, (1) moving the second pin isolation valve away from the third line, (2) moving the movable element and (3) Moving the second pin isolation valve toward the movable member so that the internal conduit within the second Pin isolation valve is connected to the second line. [132] A method of operating a flow-through injection valve according to claim 130, wherein the first and second conduits are in a surface of the moveable element, in fluid communication with a sample loop of a high pressure liquid chromatography (HPLC) system systems) stand. [133] A method of operating a flow-through injection valve according to claim 130, wherein the first and second pin isolation valves in in fluid communication with a needle and syringe of a high pressure liquid chromatography (HPLC) system. [134] A method of operating a flow-through injection valve according to claim 130, wherein the third and fourth pin isolation valves in in fluid communication with a pump and a column of a high pressure liquid chromatography (HPLC) system. [135] A method of operating a multiple valve according to claim 131, wherein the first and the second line in a End surface of the movable element of the flow injection valve, in fluid communication with a sample loop of a High Pressure Liquid Chromatography (HPLC) systems. [136] The method of operating a multiple valve of claim 131, wherein the first and second pin isolation valves of the Flow injection valve in fluid communication with a needle and syringe of a high pressure liquid chromatography (HPLC) system systems) stand. [137] Method of operating a multiple valve according to claim 131, wherein the third and fourth pin isolation valve of the Flow injection valve in fluidic communication with a pump and column of a high pressure liquid chromatography system (HPLC systems).