Novel cryopreservation tube

Abstract

A novel cryopreservation tube, characterized in that it comprises a tube body (1) and a tube cap (2), the tube body (1) and the tube cap (2) being rotatably clamped together, the tube cap (2) comprising a tube wall (3), the tube wall (3) comprising a tube body bonding layer (21), a cold shrink layer (22), and a protective layer (23) arranged sequentially from the inside to the outside, the cold shrink layer (22) being bonded to the periphery of the tube body bonding layer (21), the tube body bonding layer (21) and the bonding surface of the tube body (1) having thread structures that correspond to and are compatible with each other, the tube body bonding layer (21) having a sealing ring (24) on the inner wall near the opening of the tube cap (2).

Description

Technical FieldThe invention relates to the technical field of in vivo preservation, and more particularly to a novel cryopreservative tube.Background ArtBiocompatibility tubes are one of the most commonly used consumables in biological research and in the medical field, which are generally used for the transport and storage of tissue or cell samples at low temperatures. In recent years, with the progress of science and technology and the depression of medical research, the demand for tissue or cell sample libraries has increased greatly in China, and many biological companies and medical facilities have established their own tissue or cell sample libraries, such as stem cell libraries, tumor tissue libraries, cord blood libraries, etc. Although the sample libraries have made great advances in standardized management and quality control, they still present many difficult problems, one of which is the quality and safety of biocompatibility tubes.To protect the samples in the biocompatibility tubes from contaminants or leaks, the focus in the design of biocompatibility tubes is on the problem of sealing. To solve this problem, the biocompatibility tubes sold on the market are usually made of high-strength plastic, with tube lids of external or internal rotation containing gaskets, but the actual sealing performance is not satisfactory, above all due to the fact that the caps and tubes are made of hard plastic. The internal stresses of thermal expansion and contraction of the freezing liquid due to the temperature difference cannot be eliminated efficiently, which easily causes the liquid nitrogen to enter through the gap between the caps and tubes, which leads not only to the samples being contaminated but also to the tube bursting, which presents a potential safety risk for both the samples and the laboratory personnel.Content of the Utility ModelIn view of this, the purpose of the utility model is to provide a novel cryopreservative tube so that it can solve the problem of poor sealing performance of existing cryopreservative tube at low temperatures.The utility model solves the above technical problems by the following technical means: it comprises a tubular body and a tubular cap, the tubular body and the tubular cap being rotatably clamped together, the tubular cap comprising a tubular wall, the tubular wall comprising a tubular body connection layer, a cold shrinkage layer and a protective layer arranged one after the other from the inside to the outside, the cold shrinkage layer being connected to the periphery of the tubular body connection layer, the tubular body connection layer and the connection surface of the tubular body having thread structures which correspond to each other and are compatible, the tubular body connection layer having a sealing ring arranged on the inner wall of the tubular body connection layer near the opening of the tubular cap. The cold shrinkage layer provided in this application serves to shrink the biocompatibility tube in this apparatus at a low temperature storage to tauten the tube body connecting layer connected to the tube body so as to be closely connected to the tube body, thereby enhancing the sealing of the tube body and the tube cap in the biocompatibility tube at a low temperature state, particularly at the storage in liquid nitrogen.Further, a plurality of adsorption rings are provided on the inner wall of the tube body connecting layer in the axial direction, the axial line of the adsorption ring being coordinated with the axial line of the tube body connecting layer, a plurality of adsorption grooves corresponding to the adsorption rings being provided at the location of the tube body corresponding to the adsorption ring, the width of the adsorption grooves being smaller than the thickness of the adsorption rings. In the present application, when the biocompatibility tube is processed in the cold storage at a low temperature, the air in the adsorption groove is contracted by cold, whereby the adsorption ring located at the opening of the adsorption groove is drawn into the groove to firmly bond the tube body bonding layer bonded to the adsorption ring to the periphery of the tube body, and also cooperate with the cold shrink body in sealing the joint between the tube body and the tube cap.Furthermore, an explosion protection chamber is provided in the protective layer, wherein an explosion protection layer is provided in the explosion protection chamber. This application reduces the risk of explosion of the cryopreservative tube. More preferably, an explosion-proof layer may be applied to the tube cover and the tube body to enhance the safety of the whole biocompatibility tube.Further, the explosion-proof layer is a nylon layer or a glass fiber layer.Further, a support frame is provided at the end of the tube body remote from the tube cap. This application is provided with a fan blade shaped biocompatibility tube on the tube body to prevent the tube body from being tightly connected to the plug connector. At the same time, it is convenient for the user to screw the tube body off the receiving plate.Further, the tube body is provided with a dashed scale along the circumferential side in the height direction. This application is provided with a bar scale corresponding to the sample volume to allow users to observe the sample volume and record relevant data.Further, the tube cap comprises a tube cap, the tube cap being provided on the side in communication with the tube wall internally with a slide groove, a piston and a connecting rod, the piston being connected to the slide groove beyond the interference, one end of the connecting rod being connected to the piston, while the other end thereof is connected penetratingly to a turn table provided outside the tube cap. The present application provides a piston structure such that when the piston moves outward, the volume of air in the tubular body expands so that the tubular body is a vacuum environment, thereby initially sealing the seal.Furthermore, the connecting rod is screwed to the tube cover. The presence of the threaded structure prevents the piston from moving under the action of negative pressure. Thus, the stability of the vacuum environment is ensured.Thus, the utility model has advantages as follows: 1. the cold shrinkage layer provided in this application serves to shrink the biocompatibility tube in this device at a low temperature storage to tauten the tube body connecting layer connected to the tube body so as to be closely connected to the tube body, thereby enhancing the sealing of the tube body and the tube cap in the biocompatibility tube at a low temperature condition, particularly at the liquid nitrogen storage. 2. in the present application, when the biocompatibility tube is processed in the cooling storage at a low temperature, the air in the adsorption groove is contracted by cold, whereby the adsorption ring located at the opening of the adsorption groove is drawn into the groove to firmly bond the tube body bonding layer bonded to the adsorption ring to the periphery of the tube body, and also cooperate with the cold shrink body in sealing the joint between the tube body and the tube cap. 3. the present application provides a piston structure such that when the piston moves outward, the air volume in the tubular body expands so that the tubular body is a vacuum environment, thereby initially sealing off the seal.Illustration of the Accompanying DrawingsFIG. 1 is a schematic view showing the overall structure of the cryopreservative tube of the invention; FIG. 2 is a schematic view showing the connection part between the tube body and the tube cap of the utility model; FIG. 3 is a cross-sectional view of the tube cap of the utility model.Herein: 1: tube body; 11: adsorption groove; 12: support frame; 2: tube cap; 21: tube body connection layer; 22: cold shrinkage layer; 23: protection layer; 24: seal ring; 25: adsorption ring; 26: explosion protection chamber; 3: tube wall; 4: tube cap; 41: sliding groove; 42: piston; 43: connecting rod; 44: turntable; 5: explosion protection layer.Specific EmbodimentsThe following embodiments of the present invention will be illustrated by specific embodiments, and those skilled in the art can understand the advantages and effectiveness of the present invention based on the contents disclosed in this specification. It should be noted that the drawings in the following embodiments are for illustrative purposes only and represent schematic drawings only, not physical drawings, and may not be construed as limitations of the present invention. In order to better illustrate the embodiments of the present invention, certain parts are omitted, enlarged or reduced in the drawings. It will be understood by those skilled in the art that certain well-known structures may be omitted from the drawings and descriptions thereof.The same or similar labels in the drawings of the embodiment of the invention correspond to the same or similar parts. In describing the utility model, it is to be understood that, in the terms "upper", "lower", "left", "right", "front", "rear", etc., the orientation or positional relationship indicated is based on the orientation or positional relationship shown in the accompanying drawings or on the orientation or positional relationship in which the subject matter of application is usually placed in use, and is merely for facilitating and simplifying the description of the application and is not intended to indicate or imply that the referenced arrangement or component has a particular orientation and must be constructed and operated in a particular manner. Therefore, the terms describing the positional relationship in the figure are used only for illustrative purposes, and should not be construed as limiting the utility model. For ordinary skilled technical staff, the specific meaning of the above terms can be understood according to specific circumstances.A novel biocompatibility tube as shown in Figs. 1 and 3 comprises a tube body 1 and a tube cap 2. the tube body 1 is for receiving samples and the tube cap 2 is for closing the tube body 1. the tube body 1 and the tube cap 2 are rotatably clamped together. The joint surface of the tubular body 1 and the tubular cap 2 have thread structures which correspond to each other and are compatible so that the tubular body 1 and the tubular cap 2 are screwed to each other. The tube cap 2 includes a tube wall 3 and a tube cap 4. the tube wall 3 includes a tube body connection layer 21, a cold shrinkage layer 22, and a protection layer 23. the tube body connection layer 21, the cold shrinkage layer 22, and the protection layer 23 are sequentially arranged from the inside to the outside.Specifically, as in FIG. 3, the tube body connection layer 21 is for engaging with the tube body 1, and the tube body connection layer 21 and the joint surface of the tube body 1 have thread structures that are the same as each other and are compatible. The cold shrinkage layer 22 is bonded to the periphery of the tube body bonding layer 21. The cold shrinkage layer 22 serves to shrink the biocompatibility tube in this apparatus at a low temperature storage to tauten the tube body connecting layer 21 connected to the tube body 1 so as to be closely connected to the tube body 1, thereby enhancing the sealing of the tube body 1 and the tube cap 2 in the biocompatibility tube at a low temperature state, particularly at the storage in liquid nitrogen.The tube body connecting layer 21 has a seal ring 24 disposed near the opening of the tube cap 2. The diameter of the sealing ring 24 is matched to the inner diameter of the tube cap 2. The seal ring 24 is used so that the tube body connecting layer 21 is connected to the tube body 1 beyond the interference when it is firmly connected to the tube body 1 under the systolic pressure of the cold shrinkage layer 22. When the temperature further decreases, the seal ring 24 is more closely bonded to the tube body 1 under the pressure of the cold shrinkage layer 22, thereby enhancing the sealing of the tube cap 2 in the vicinity of the opening.A plurality of adsorption rings 25 are provided on the inner wall of the tube body joining layer 21 in the axial direction. The axial line of the adsorption ring 25 is coordinated with the axial line of the tube body connection layer 21. As in FIG. 2, a plurality of adsorption grooves 11 corresponding to the adsorption rings 25 are provided on the periphery of the tube body 1, the number of which is accordingly. The adsorption groove 11 and the adsorption ring 25 are set so that when the tube body 1 and the tube cap 2 are rotated and tightened, the adsorption ring 25 can be engaged with the adsorption groove 11. The width of the adsorption grooves 11 is smaller than the thickness of the adsorption rings 25. that is, when the adsorption ring 25 is moved to snap with the adsorption groove 11, the adsorption ring 25 can completely seal the adsorption groove 11. The air in the adsorption groove 11 is contracted by cold when the biocompatibility tube is processed in the cooling storage at a low temperature, whereby the adsorption ring 25 located at the opening of the adsorption groove 11 is drawn into the groove to firmly bond the tube body bonding layer 21 bonded to the adsorption ring 25 to the periphery of the tube body 1. In order to facilitate the adsorption of the adsorption groove 11, the adsorption ring 25 may preferably be a soft material having a certain deformation capability, such as a silicone ring or a rubber ring.The protective layer 23 is the outermost layer of the tube cap 2 serving to provide an explosion-proof chamber 26 in the protective layer 23. An explosion protection layer 5 is provided in the explosion protection chamber 26. Preferably, the explosion-proof layer 5 is a nylon layer or a glass fiber layer. In this application, in order to prevent liquid nitrogen from entering the gap between the tube body 1 and the tube cap 2 during preservation of the biocompatibility tube, causing the risk of tube explosion, an explosion-proof layer 5 is provided in the protective layer 23 to reduce the risk of explosion of the biocompatibility tube. More preferably, an explosion-proof layer 5 may be applied to the tube cover 4 and the tube body 1 to enhance the safety of the whole biocompatibility tube.A support frame 12 is provided at the end of the tubular body 1 remote from the tubular cap 2. The support frame 12 is a fan blade structure. When using the biocompatibility tube, it is usually inserted into the corresponding receiving plate with a plug connection for a plurality of biocompatibility tubes to be stored simultaneously. In order to prevent the tube body 1 and the plug terminal from being closely connected to each other due to the principle of thermal expansion and contraction, this application is provided with a fan blade-shaped biocompatibility tube on the tube body 1 to prevent the tube body 1 from being closely connected to the plug terminal. At the same time, it is convenient for the user to screw off the tubular body 1 from the receiving plate.The tubular body 1 is provided along the circumferential side in the height direction with a dashed scale for illustrating the sample volume in the tubular body 1. It is facilitated that users can observe the sample volume and record relevant data.The tube cover 4 is provided on the side in communication with the tube wall 3 internally with a slide groove 41, a piston 42 and a connecting rod 43, the piston 42 being connected to the slide groove 41 beyond the interference. One end of the connecting rod 43 is connected to the piston 42, while the other end thereof is connected to a rotary plate 44 provided outside the tubular cover 4 penetrating through the slide groove 41 as far as outside the tubular cover 4. The turntable 44 is capable of pulling the piston 42 by the connecting rod 43 so that the piston 42 slides in the slide groove 41. When the piston 42 moves outward, the volume of air in the tubular body 1 expands so that the tubular body 1 is a vacuum environment, thereby first completing the sealing. The connection between the connecting rod 43 and the tube cover 4 is further designed as threaded structures which correspond to one another and are compatible in order to prevent the piston 42 from slipping into the tube under the action of negative pressure.The use of this utility model is as follows: When the tubular body 1 and the tubular cap 2 of the biocompatibility tube are tightened before being stored in the low temperature environment, the turntable 44 moves the piston to make the tubular body 1 form a vacuum environment to complete the initial sealing. As the temperature further decreases, the volume of the cold shrinkage layer 22 decreases to the extent that it presses the tube body joining layer 21 joined to the cold shrinkage layer 22, firmly fixing the tube body joining layer 21 to the tube body 1 under the pressure of the cold shrinkage layer 22. The contraction of the cold shrinkage layer 22 enables the tube cap 2 and the tube body 1 to be held firmly contracted even if the internal stress at low temperatures cannot be effectively removed, thereby greatly improving the sealing. Moreover, at low temperatures, the adsorption groove 11 also draws a part of the adsorption ring 25 into the groove used in conjunction with the cold shrinkage layer 22 for sealing the gaps at the joint between the tube body 1 and the tube cap 2. In this application, an explosion-proof layer is also provided to reduce the risk of explosion of the biocompatibility tube and to increase the safety of the biocompatibility tube during use.The above embodiments are only for illustrating the technical solutions of the utility model and are not intended to limit the same. Although the detailed description of the utility model with reference to the preferred embodiment, it should be understood by those skilled in the art that it is still possible to make changes or equivalent substitutions in the technical solution of the utility model, and that these changes or equivalent substitutions do not result in the changed technical solution departing from the purpose and scope of the technical solution of the utility model, so that they are all covered by the scope of the claims of the utility model. The technique, shape and construction of the utility model, which will not be described in detail, are all known techniques.A novel biocompatibility tube comprises a tube body and a tube cap, the tube body and the tube cap being rotatably clamped together, the tube cap comprising a tube wall, the tube wall comprising a tube body connection layer, a cold shrinkage layer and a protective layer arranged one after the other from the inside to the outside, the cold shrinkage layer being connected to the periphery of the tube body connection layer, the tube body connection layer and the connection surface of the tube body having thread structures that correspond to each other and are compatible, the tube body connection layer having a sealing ring arranged on the inner wall of the tube body connection layer near the opening of the tube cap. The cold shrinkage layer provided serves to shrink the biocompatibility tube in this apparatus at a low temperature storage to tauten the tube body connection layer connected to the tube body so as to be closely connected to the tube body, thereby enhancing the sealing of the tube body and the tube cap in the biocompatibility tube at a low temperature state, particularly at the liquid nitrogen storage.

Claims

A novel biocompatibility tube, characterized in that it comprises a tube body (1) and a tube cap (2), said tube body (1) and said tube cap (2) being rotatably clamped together, said tube cap (2) comprising a tube wall (3), said tube wall (3) comprising a tube body connection layer (21), a cold shrinkage layer (22) and a protective layer (23), which are arranged one after the other from inside to outside, said cold shrinkage layer (22) being connected to the periphery of said tube body connection layer (21), said tube body connection layer (21) and the connection surface of said tube body (1) having thread structures corresponding to each other and compatible, said tube body connection layer (21) having on the inner wall a sealing ring (24) near the opening of said tube cap (2).The novel biocompatibility tube according to claim 1, characterized in that a plurality of adsorption rings (25) are provided on the inner wall of the tube body connection layer (21) in the axial direction, the axial line of the adsorption ring (25) being coordinated with the axial line of the tube body connection layer (21), a plurality of adsorption grooves (11) corresponding to the adsorption rings (25) being provided at the location of the tube body (1) corresponding to the adsorption ring (25), the width of the adsorption grooves (11) being smaller than the thickness of the adsorption rings (25).Novel biocompatibility tube according to claim 2, characterised in that an explosion protection chamber (26) is provided in the protective layer (23), wherein an explosion protection layer (5) is provided in the explosion protection chamber (26).Novel biocompatibility tube according to claim 3, characterized in that the explosion protection layer (5) is a nylon layer or a glass fibre layer.Novel biocompatibility tube according to claim 4, characterised in that a support frame (12) is provided at the end of the tube body (1) remote from the tube cap (2).The novel biocompatibility tube according to claim 5, characterized in that the tube body (1) is provided with a dashed scale along the circumferential side in the height direction.The novel biocompatibility tube according to claim 6, characterized in that the tube cap (2) further comprises a tube cap (4), the tube cap (4) being provided on the side in connection with the tube wall (3) internally with a slide groove (41), a piston (42) and a connecting rod (43), the piston (42) being connected to the slide groove (41) beyond the interference, one end of the connecting rod (43) being connected to the piston (42), while the other end thereof being connected penetrating the tube cap (4) to a rotary plate (44) provided outside the tube cap (4).Novel biocompatibility tube according to claim 7, characterised in that the connecting rod (43) is screwed to the tube cover (4).

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