METHOD FOR CULTURING MAMMAL CELLS
Patent Information
- Application Number
- DE602015092994
- Authority / Receiving Office
- DE · DE
- Patent Type
- Patents
- Current Assignee / Owner
- Priority Date
- 2014-12-22
- Filing Date
- 2015-12-21
- Publication Date
- 2026-02-11
- Estimated Expiration
- 2035-12-21
AI Technical Summary
Current methods for culturing mammalian cells to produce recombinant proteins are limited by the lack of flexibility and efficiency, particularly in achieving high cell densities and maintaining protein stability, especially with the use of poloxamer-188 concentrations above 5 g/L.
The method involves culturing mammalian cells with poloxamer-188 concentrations ranging from 1.8 g/L to 10.0 g/L, adjusting based on factors like pore size, pore type, gas flow rate, viable cell density, and cell stress markers, and using perfusion bioreactors to maintain optimal conditions for cell growth and protein production.
This approach enables high cell densities and improved protein production rates, achieving viable cell densities up to 200 x 10^6 cells/mL and maintaining protein stability, thus enhancing the efficiency and flexibility of recombinant protein manufacturing.