DETECTION OF MYCOBACTERIUM SPECIES

DE602020057488T2Active Publication Date: 2025-08-27QUEENS UNIV OF BELFAST
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Patent Information

Application Number
DE602020057488
Authority / Receiving Office
DE · DE
Patent Type
Patents
Current Assignee / Owner
Priority Date
2019-09-23
Filing Date
2020-09-23
Publication Date
2025-08-27
Estimated Expiration
2040-09-23

AI Technical Summary

Technical Problem

Current methods for detecting viable Mycobacterium species, such as Mycobacterium avium complex and Mycobacterium tuberculosis, are labor-intensive, costly, and difficult to scale up for high-throughput analysis, and struggle to differentiate between live and dead cells, which is crucial for accurate disease diagnosis and food safety.

Method used

A phage-based method using mycobacteriophage-coupled paramagnetic particles for selective capture and lysis of viable Mycobacterium species, followed by nucleic acid analysis to identify specific sequences, allowing rapid and reliable detection in a single-tube format.

Benefits of technology

Enables rapid, cost-effective, and high-throughput detection of viable Mycobacterium species, distinguishing between infected and vaccinated individuals, and differentiating between live and dead cells, with results obtainable within 24 hours.

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