Treatment of nsclc patients with tumor infiltrating lymphocyte therapies

Abstract

The present invention provides improved and / or shortened processes and methods for preparing TILs in order to prepare therapeutic populations of TILs with increased therapeutic efficacy for the treatment of cancer, including for example, non-small cell lung carcinoma (NSCLC), wherein the NSCLC is refractory to treatment with an anti-PD-1 antibody and / or anti-PD-Ll antibody and / or VEGF inhibitor, and / or wherein the cancer, including for example or NSCLC, has a predetermined tumor proportion score (TPS), and including when the TILs for expansion are obtained prior to the patient receiving a cancer treatment.

Description

TREATMENT OF NSCLC PATIENTS WITH TUMOR INFILTRATING LYMPHOCYTETHERAPIESCROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application claims priority to U.S. Provisional Application No 63 / 328,236, filed April 6, 2022, of which is herein incorporated by reference in its entirety.BACKGROUND OF THE INVENTION

[0002] A significant unmet need exists for new treatment options for patients with locally advanced or metastatic non-small-cell lung cancer (NSCLC); these patients comprise approximately 70% of newly diagnosed patients with NSCLC (Molina JR, Yang P, Cassivi SD, Schild SE, Adjei AA. Mayo Clin Proc. 2008; 83(5):584-94). Lung cancer is the leading cause of cancer deaths worldwide, with approximately 1.7 million deaths reported in 2015. Of the lung cancer deaths, > 80% were attributed to non-small-cell lung cancer (NSCLC) (21). It was expected that in 2020, there would have been an estimated 228,820 new cases and 135,720 deaths attributed to lung and bronchus cancer in the United States (Siegel RL, 2015. CA Cancer J Clin. 2015; 65(l):5-29). Thus, despite the approval of checkpoint inhibitors (CPIs), which revolutionized NSCLC treatment and outcomes, there remains a significant unmet medical need in NSCLC.

[0003] Overall, in men and women, the lifetime risk of developing lung cancer regardless of smoking status is approximately 1 in 14 and 1 in 17, respectively. However, the risk of developing lung cancer is much higher in smokers vs. nonsmokers, e.g., men who smoke are 23 times more likely to develop cancer; likewise, women who smoke are 13 times more likely to develop lung cancer than those who do not (American Lung Association - Lung cancer fact sheet 2017 [American Lung Association - Lung cancer fact sheet]; available from: the World Wide Web at lung.org / lung-health-and- diseases / lung-disease-lookup / lung-cancer / resource-library / lung-cancer-fact-sheet.html).

[0004] Prior to checkpoint inhibitors, platinum doublet chemotherapy was utilized in the initial treatment of patients with incurable NSCLC (Schiller J. H. et al. 2002 N Engl J Med 346 (2):92-8.), and it produced an objective response rate (ORR) of 20% to 30% with limited durability. Pembrolizumab, alone or in combination with cytotoxic therapy, revolutionized the treatment, producing higher response rates that have proven more durable as well (Gandhi Leena et al. 2018 N EnglJMed M (22):2078-2092; Paz-Ares L. et al. 2018 N Engl JMedM9 (21):2040-2051; Viteri S. et al. 2020 Transl Lung Cancer Res 9 (3):828-832; Pacheco J. M. 2020 Transl Lung Cancer Res 9 (1):148-153; Mok T. S. K. et al. 2019 Lancet 393 (10183):1819-1830; Nosaki K. et al. 2019 Lung Cancer 135:188-195; Morgcnsztcrn D. 2019 J Thorac Dis 11 (Suppl 15):S1963-S1965 ; Reck M. etal. 2016 N Engl J Med 375 (19): 1823-1833). Other CPIs have demonstrated improved ORR and durability in combination with chemotherapy in the first-line setting for NSCLC (Socinski MA et al 2018 New Engl J Med 378:2288-301). Despite these impressive results, there are few complete responses; almost all patients either fail to respond or progress, thus necessitating subsequent therapy.

[0005] For NSCLC patients with identified driver mutations, the preferred option is treatment with targeted TKIs directed against the relevant mutation (e.g., osimertinib for epidermal growth factor receptor [EGFR] mutations, ceritinib for ALK mutations or crizotinib for ROS-1 mutations). For previously untreated patients with NSCLC whose tumors express PD-L1, the available treatment options include pembrolizumab monotherapy (commonly used only for patients with tumor proportion score (TPS) for PD-L1 expression of at least 50%) or pembrolizumab in combination with chemotherapy. For patients with NSCLC and PD-L1 expression < 50%, the preferred option is the combination of pemetrexed, carboplatin or cisplatin, and pembrolizumab. Patients with TPS for PD- L1 < 1% and no actionable mutations have no viable treatment options and are not candidates for PD- 1 or PD-L1 CPIs. The combination of platinum-based doublet chemotherapy, bevacizumab, and atezolizumab is another potential therapeutic alternative in patients with NSCLC, as is a combination of nivolumab, ipilimumab and cytotoxic therapy (Hellmann et al. 2019 New Engl J Med 381 2020- 31).

[0006] Once a patient has disease progression on checkpoint inhibitor therapy plus chemotherapy, treatment options are limited and suffer from low efficacy, with objective response rate ~ 10% and short progression-free survival (PFS), as well as high toxicity. The most commonly used agent is docetaxol, though other single agent cytotoxics or cytotoxics combined with VEGF inhibitors are sometimes employed, but all have similar, poor, outcomes. Thus, there is an urgent need for better therapeutic options in the second line treatment following checkpoint inhibitor therapy plus chemotherapy.

[0007] Furthermore, current TIL manufacturing and treatment processes are limited by length, cost, sterility concerns, and other factors described herein such that the potential to treat patients which are refractory to anti-PD-1 and / or anti-PD-Ll and / or VEGF inhibitor treatments and as such have been severely limited. There is an urgent need to provide TIL manufacturing processes and therapies based on such processes that are appropriate for use in treating patients for whom very few or no viable treatment options remain. The present invention meets this need by providing a shortened manufacturing process for use in generating TILs which can then be employed in the treatment of refractory and / or progressive non-small cell lung carcinoma (NSCLC) patients.BRIEF SUMMARY OF THE INVENTION

[0008] In some embodiments, the present invention provides a method of making a cryopreserved population of TILs from a NSCLC patient, wherein the patient is naive to a cancer treatment, the method comprising:(a) prior to the patient receives a cancer treatment, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cryoprcscrving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest; wherein the patient subsequently receives an ICI treatment and / or a standard of care treatment; optionally wherein the first population of TILs is further expanded into a therapeutic population of TILs if the patient exhibits progressive disease on or after the ICI and / or standard of care treatment.

[0009] In some embodiments, the present invention provides a method of making a cryopreserved population of TILs from a NSCLC patient, wherein the patient is on a maintenance therapy that is interrupted, the method comprising:(a) prior to the resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cry opreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest; wherein the patient subsequently resumes the maitnainence therapy; optionally wherein the first population of TILs is further expanded into a therapeutic population of TILs if the patient exhibits progressive disease on or after the resumption of the maintenance therapy.

[0010] In some embodiments, the present invention provides a method of making a cryopreserved population of TILs from a NSCLC patient, wherein the patient is naive to a cancer treatment, comprising:(a) prior to the patient receives a cancer treatment, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cry opreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) thawing the cryopreserved tumor fragments or tumor digest and adding the first population of TILs into a closed system;(d) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-14 days to obtain the second population of TILs, and wherein the transition from step (c) to step (d) occurs without opening the system;(e) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-14 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the second expansion is performed in a closed container providing a second gas-permeable surface area, and wherein the transition from step (d) to step (e) occurs without opening the system;(f) harvesting therapeutic population of TILs obtained from step (e), wherein the transition from step (e) to step (I) occurs without opening the system; and(g) transferring the harvested TIL population from step (f) to an infusion bag, wherein the transfer from step (f) to (g) occurs without opening the system; and(h) cryoprcscrving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process.

[0011] In some embodiments, the present invention provides a method of making a cryopreserved population of TILs from a NSCLC patient, wherein the patient is naive to a cancer treatment, comprising:(a) prior to the patient receives a cancer treatment, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cry opreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) thawing the cryoprcscrvcd tumor fragments or tumor digest and adding the first population of TILs into a closed system;(d) performing a priming first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2, OKT-3, and antigen presenting cells (APCs) to produce a second population of TILs, wherein the priming first expansion is performed in a container comprising a firstgas-permeable surface area, wherein the priming first expansion is performed for first period of about 1 to 7 / 8 days to obtain the second population of TILs, wherein the second population of TILs is greater in number than the first population of TILs;(c) performing a rapid second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and APCs, to produce a third population of TILs, wherein the number of APCs added in the rapid second expansion is at least twice the number of APCs added in step (b), wherein the rapid second expansion is performed for a second period of about 1 to 11 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the rapid second expansion is performed in a container comprising a second gas-permeable surface area;(f) harvesting therapeutic population of TILs obtained from step (e), wherein the transition from step (e) to step (f) occurs without opening the system; and(g) transferring the harvested TIL population from step (f) to an infusion bag, wherein the transfer from step (f) to (g) occurs without opening the system; and(h) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process.

[0012] In some embodiments, the rapid expansion can proceed for 1 day, 2 days, 3 days, 4, days, 5 days, 6 days, 7 days, 8 days, 9 days or 10 days after initiation of the rapid expansion.

[0013] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a NSCLC patient, wherein the patient is on maintenance therapy that is interrupted, comprising:(a) prior to the resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cry opreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) thawing the cryopreserved tumor fragments or tumor digest and adding the first population of TILs into a closed system;(d) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performedfor about 3-14 days to obtain the second population of TILs, and wherein the transition from step (c) to step (d) occurs without opening the system;(e) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-14 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the second expansion is performed in a closed container providing a second gas-permeable surface area, and wherein the transition from step (d) to step (e) occurs without opening the system;(f) harvesting therapeutic population of TILs obtained from step (e), wherein the transition from step (e) to step (f) occurs without opening the system; and(g) transferring the harvested TIL population from step (f) to an infusion bag, wherein the transfer from step (f) to (g) occurs without opening the system; and(h) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cryoprcscrvation process.

[0014] In some embodiments, the present disclosures provide a method of making a crvoprcscrvcd population of TILs from a NSCLC patient, wherein the patient is on maintenance therapy that is interrupted, comprising:(a) prior to the resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cry opreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) thawing the cryopreserved tumor fragments or tumor digest and adding the first population of TILs into a closed system;(d) performing a priming first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2, OKT-3, and antigen presenting cells (APCs) to produce a second population of TILs, wherein the priming first expansion is performed in a container comprising a first gas-permeable surface area, wherein the priming first expansion is performed for first period of about 1 to 7 / 8 days to obtain the second population of TILs, wherein the second population of TILs is greater in number than the first population of TILs;(e) performing a rapid second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and APCs, to produce a third population of TILs,wherein the number of APCs added in the rapid second expansion is at least twice the number of APCs added in step (b), wherein the rapid second expansion is performed for a second period of about 1 to 11 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the rapid second expansion is performed in a container comprising a second gas-permeable surface area;(f) harvesting therapeutic population of TILs obtained from step (e), wherein the transition from step (e) to step (f) occurs without opening the system; and(g) transferring the harvested TIL population from step (f) to an infusion bag, wherein the transfer from step (f) to (g) occurs without opening the system; and(h) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process.

[0015] In some embodiments, the cancer patient is naive to any cancer treatment.

[0016] In some embodiments, the patient is naive to ICI treatment, anti-VEGF treatment, or chemotherapeutic treatment.

[0017] In some embodiments, the patient is on maintenance therapy that is interrupted at the time of step (a), and the maintenance therapy is resumed after step (a).

[0018] In some embodiments, the patient is in a washout period at initiation of step (a).

[0019] In some embodiments, step of thawing and the subsequent steps are performed after die patient receives the cancer treatment or resumes the maintenance therapy and the patient exhibits progressive disease.

[0020] In some embodiments, the patient exhibits progressive disease at least about 1 week, 2 weeks, 3 weeks, 1 month, 2 months, 3 month, 4 months, 5 months, 6 months, 7 months, 8 months, 9 months, 10 months, 11 months, 12 months, 13 months, 14 months, 15 months, 16 months, 17 months, 18 months, 19 months, 20 months, 21 months, 22 months, 23 months, 24 months, 25 months, 26 months, 27 months, 28 months, 29 months, 30 months, 31 months, 32 months, 33 months, 34 months, 35 months, 36 months, after the step (b) of cryopreserving.

[0021] In some embodiments, the patient receives or is going to receive a first-line ICI treatment and / or standard treatment.

[0022] In some embodiments, the patient receives or is going to receive a second-line ICI treatment and / or standard treatment.

[0023] In some embodiments, the patient is diagnosed with NSCLC.

[0024] In some embodiments, the patient is diagnosed with metastatic stage IV NSCLC.

[0025] In some embodiments, the subject or patient has at least one of: i) a predetermined tumor proportion score (TPS) of PD-L1 of < 1%, ii) a TPS score of PD-L1 of l%-49%, or iii) a predetermined absence of one or more driver mutations.

[0026] In some embodiments, the patient or subject has a TPS of PD-L1 of l%-49%.

[0027] In some embodiments, the patient or subject has a TPS of PD-L1 of < 1% and has a predetermined absence of one or more driver mutations.

[0028] In some embodiments, the one or more driver is selected from the group consisting of an EGFR mutation, an EGFR insertion, EGFR exon20, a KRAS mutation, a BRAF -mutation, a BRAF V600 mutation, an ALK-mutation, a c-ROS-mutation (ROS 1 -mutation), a ROS1 fusion, a RET mutation, a RET fusion, an ERBB2 mutation, an ERBB2 amplification, a BRCA mutation, a MAP2K1 mutation, PIK3CA, CDKN2A, a PTEN mutation, an UMD mutation, an NRAS mutation, a KRAS mutation, an NF1 mutation, a MET mutation, a MET splice and / or altered MET signaling, a TP53 mutation, a CREBBP mutation, a KMT2C mutation, a KMT2D mutation, an ARID 1 A mutation, a RBI mutation, an ATM mutation, a SETD2 mutation, a FLT3 mutation, a PTPN11 mutation, a FGFR1 mutation, an EP300 mutation, a MYC mutation, an EZH2 mutation, a JAK2 mutation, a FBXW7 mutation, a CCND3 mutation, and a GNA11 mutation.

[0029] In some embodiments, the patient or subject has a TPS of < 1% and has a NSCLC that is not indicated for treatment by an EGFR inhibitor, a BRAF inhibitor, an ALK inhibitor, a c-Ros inhibitor, a RET inhibitor, an ERBB2 inhibitor, BRCA inhibitor, a MAP2K1 inhibitor, PIK3CA inhibitor, CDKN2A inhibitor, a PTEN inhibitor, an UMD inhibitor, an NRAS inhibitor, a KRAS inhibitor, an NF1 inhibitor, MET inhibitor a TP53 inhibitor, a CREBBP inhibitor, a KMT2C inhibitor, a KMT2D mutation, an ARID 1 A mutation, a RBI inhibitor, an ATM inhibitor, a SETD2 inhibitor, a FLT3 inhibitor, a PTPN11 inhibitor, a FGFR1 inhibitor, an EP300 inhibitor, a MYC inhibitor, an EZH2 inhibitor, a JAK2 inhibitor, a FBXW7 inhibitor, a CCND3 inhibitor, and a GNA11 inhibitor.

[0030] In some embodiments, the one or more driver mutations comprises a genomic alteration of EGFR, ALK, or ROS.

[0031] In some embodiments, the one or more driver mutations consists of a genomic alteration of EGFR, ALK, or ROS.

[0032] In some embodiments, the NSCLC has low or no expression of PD-L1.

[0033] In some embodiments, the patient has a predetermined absence of one or more driver mutations.

[0034] In some embodiments, the NSCLC is refractory or resistant to treatment with a chemotherapeutic agent.

[0035] In some embodiments, the NSCLC is refractory or resistant to treatment with a VEGF-A In some embodiments, the NSCLC has been treated with a chemotherapeutic agent but is not being currently treated with a chemotherapeutic agent.

[0036] In some embodiments, the NSCLC has been treated with a chemotherapeutic agent but is not being currently treated with a chemotherapeutic agent and has a TPS of < 1%.

[0037] In some embodiments, the NSCLC has been treated with a VEGF-A inhibitor but is not being currently treated with a VEGF-A inhibitor.

[0038] In some embodiments, the NSCLC has been treated with a VEGF-A inhibitor but is not being In some embodiments, the NSCLC has been treated with a chemotherapeutic agent and / or a VEGF-A inhibitor, but is not being currently treated with a chemotherapeutic agent and / or a VEGF-A inhibitor.

[0039] In some embodiments, the NSCLC has been treated with a chemotherapeutic agent and / or a VEGF-A inhibitor but is not being currently treated with a chemotherapeutic agent and / or a VEGF-A inhibitor and has a TPS of < 1%.

[0040] In some embodiments, the NSCLC has not been previously treated with an anti-PD-1 and / or anti-PD-Ll antibody.

[0041] In some embodiments, the NSCLC has not been previously treated with an anti-PD-1 and / or anti-PD-Ll antibody and has been previously treated a chemotherapeutic agent and / or a VEGF-A inhibitor.

[0042] In some embodiments, the NSCLC has not been previously treated with an anti-PD-1 and / or anti-PD-Ll antibody and has been previously treated a chemotherapeutic agent and / or a VEGF-A inhibitor but is not being currently treated with a chemotherapeutic agent and / or a VEGF-A inhibitor.

[0043] In some embodiments, the NSCLC has not been previously treated with an anti-PD-1 and / or anti-PD-Ll antibody and has been previously treated a VEGF-A inhibitor but is not being currently treated with a VEGF-A inhibitor.

[0044] In some embodiments, the NSCLC has not been previously treated with an anti-PD-1 and / or anti-PD-Ll antibody and has been previously treated a chemotherapeutic agent and / or a VEGF-A inhibitor but is not being currently treated with a chemotherapeutic agent and / or a VEGF-A inhibitor.

[0045] In some embodiments, the NSCLC has not been previously treated with an anti-PD-1 and / or anti-PD-Ll antibody and has low or no expression of PD-L1.

[0046] In some embodiments, the NSCLC has not been previously treated with an anli-PD-1 and / or anti-PD-Ll antibody and has been previously treated a chemotherapeutic agent and / or a VEGF-A inhibitor but is not being currently treated with a chemotherapeutic agent and / or a VEGF-A inhibitor and has a TPS of < 1%.

[0047] In some embodiments, the NSCLC has been previously treated with an anti-PD-1 and / or anti- PD-Ll and / or anti-PD-L2 antibody.

[0048] In some embodiments, the NSCLC has been previously treated with an anti-PD-1 and / or anti- PD-Ll antibody and has been previously treated a chemotherapeutic agent and / or a VEGF-A inhibitor.

[0049] In some embodiments, the NSCLC is refractory or resistant to treatment with an anti-PD-1 and / or anti-PD-Ll antibody.

[0050] In some embodiments, the NSCLC has been previously treated with an anti-PD-1 and / or anti- PD-Ll antibody and the tumor proportion score was determined prior to the anti-PD-1 and / or anti- PD-Ll antibody treatment.

[0051] In some embodiments, the NSCLC has been previously treated with an anti-PD-Ll antibody and the tumor proportion score was determined prior to the anti-PD-Ll antibody treatment, or the NSCLC has been previously treated with an anti-PD-1 antibody and the tumor proportion score was determined prior to the anti-PD-1 antibody treatment.

[0052] In some embodiments, the NSCLC has been treated with a chemotherapeutic agent and / or a VEGF-A inhibitor.

[0053] In some embodiments, the NSCLC has not been previously treated with an anti-PD-1 and / or anti-PD-Ll antibody and has bulky disease at baseline.

[0054] In some embodiments, the NSCLC has been previously treated with an anti-PD-1 and / or anti- PD-Ll antibody and has bulky disease at baseline.

[0055] In some embodiments, the NSCLC has been treated with a chemotherapeutic agent and has bulky disease at baseline.

[0056] In some embodiments, the NSCLC has been treated with a chemotherapeutic agent and / or VEGF-A inhibitor but is not being currently treated with a chemotherapeutic agent and / or VEGF-A inhibitor and has bulky disease at baseline.

[0057] In some embodiments, bulky disease is indicated where the maximal tumor diameter is greater than 7 cm measured in either the transverse or coronal plane or swollen lymph nodes with a short-axis diameter of 20 mm or greater.

[0058] In some embodiments, the NSCLC is refractory or resistant to at least two prior systemic treatment courses, not including neo-adjuvant or adjuvant therapies.

[0059] In some embodiments, the NSCLC is refractory or resistant to an anti-PD-1 or an anti-PD-Ll antibody selected from the group consisting of nivolumab, pembrolizumab, JS001, TSR-042, pidilizumab, BGB-A317, SHR-1210, REGN2810, MDX-1106, PDR001, anti-PD-1 from clone: RMP1-14, anti-PD-1 antibodies disclosed in U.S. Patent No. 8,008,449, durvalumab, atezolizumab, avelumab, and fragments, derivatives, variants, as well as biosimilars thereof.

[0060] In some embodiments, the NSCLC is refractory or resistant to pembrolizumab or a biosimilar thereof.

[0061] In some embodiments, the NSCLC is refractory or resistant to nivolumab or a biosimilar thereof.

[0062] In some embodiments, the NSCLC is refractory or resistant to an anti-CTLA-4 antibody.

[0063] In some embodiments, the NSCLC is refractory or resistant to an anti- CTLA -4 antibody and pembrolizumab or a biosimilar thereof.

[0064] In some embodiments, the NSCLC is refractory or resistant to an anti- CTLA -4 antibody, and nivolumab or a biosimilar thereof.

[0065] In some embodiments, the anti-CTLA-4 antibody is ipilimumab or a biosimilar thereof.

[0066] In some embodiments, the NSCLC is refractory or resistant to durvalumab or a biosimilar thereof.

[0067] In some embodiments, the NSCLC is refractory or resistant to atezolizumab or a biosimilar thereof.

[0068] In some embodiments, the NSCLC is refractory or resistant to avelumab or a biosimilar thereof.

[0069] In some embodiments, the chemotherapeutic agent is a platinum doublet chemotherapeutic agent(s).

[0070] In some embodiments, the platinum doublet chemotherapeutic agent therapy comprises: i) a first chemotherapeutic agent selected from the group consisting of cisplatin and carboplatin,ii) and a second chemotherapeutic agent selected from the group consisting of vinorelbine, gemcitabine and a taxane (including for example, paclitaxel, docetaxel or nab-paclitaxel).

[0071] In some embodiments, the chemotherapeutic agent, including the first and / or second chemotherapeutic agent, is in combination with pemetrexed.

[0072] In some embodiments, the NSCLC is refractory or resistant to a combination therapy comprising carboplalin, paclitaxel, pemetrexed, and cisplatin.

[0073] In some embodiments, the NSCLC is refractory or resistant to a combination therapy comprising carboplatin, paclitaxel, pemetrexed, cisplatin, nivolumab, and ipilimumab.

[0074] In some embodiments, the NSCLC is refractory or resistant to a VEGF-A inhibitor.

[0075] In some embodiments, the NSCLC is refractory or resistant to a VEGF-A inhibitor selected from the group consisting of bevacizumab, ranibizumab, and icrucumab.

[0076] In some embodiments, the NSCLC is refractory or resistant to bevacizumab.

[0077] In some embodiments, the NSCLC has been analyzed for the absence or presence of one or more driver mutations.

[0078] In some embodiments, one or more driver mutations are not present.

[0079] T In some embodiments, he method of any one of Claims 64 to 65, wherein the NSCLC treatment is independent of the presence or absence of one or more driver mutations.

[0080] In some embodiments, the one or more driver mutations is selected from the group consisting of an EGFR mutation, an EGFR insertion, a KRAS mutation, a BRAF-mutation, an ALK-mutation, a c-ROS-mutation a c -ROS -mutation, EML4-ALK, and MET mutation.

[0081] In some embodiments, the EGFR mutation results in tumor transformation from NSCLC to small cell lung cancer (SCLC).

[0082] In some embodiments, the NSCLC treatment is independent of the presence or absence of high-tumor mutational burden (high-TMB) and / or microsatellite instability -high (MSI-high) status.

[0083] In some embodiments, the NSCLC exhibits high-TMB and / or MSI-high status.

[0084] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, comprising:(a) prior to the patient receives a cancer treatment, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cry opreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) waiting for the patient to exhibit progressive disease on or after the cancer treatment;(d) thawing the cryopreserved tumor fragments or tumor digest and adding the first population of TILs into a closed system;(e) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-14 days to obtain the second population of TILs, and wherein the transition from step (c) to step (d) occurs without opening the system;(I) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-14 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the second expansion is performed in a closed container providing a second gas-pcrmcablc surface area, and wherein the transition from step (e) to step (I) occurs without opening the system;(g) harvesting therapeutic population of TILs obtained from step (I), wherein the transition from step (f) to step (g) occurs without opening the sy stem; and(h) transferring the harvested TIL population from step (I) to an infusion bag, wherein the transfer from step (g) to (h) occurs without opening the system; and(i) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process.

[0085] In some embodiments, the present disclosures provide a method of making a cry opreserved population of TILs from a cancer patient, wherein the patient is on maintenance therapy that is interrupted, comprising:(a) prior to the resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cryopreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) waiting for the patient to exhibit progressive disease on or after the cancer treatment;(d) thawing the cryopreserved tumor fragments or tumor digest and adding the first population of TILs into a closed system;(e) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-14 days to obtain the second population of TILs, and wherein the transition from step (c) to step (d) occurs without opening the system;(f) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-14 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the second expansion is performed in a closed container providing a second gas-permeable surface area, and wherein the transition from step (e) to step (f) occurs without opening the system;(g) harvesting therapeutic population of TILs obtained from step (f), wherein the transition from step (f) to step (g) occurs without opening the sy stem; and(h) transferring the harvested TIL population from step (f) to an infusion bag, wherein the transfer from step (g) to (h) occurs without opening the system; and(i) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process.

[0086] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, comprising:(a) prior to the patient receives a cancer treatment, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cry opreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) waiting for the patient to exhibit progressive disease on or after the cancer treatment;(d) thawing the cryopreserved tumor fragments or tumor digest and adding the first population of TILs into a closed system;(e) performing a priming first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2, OKT-3, and antigen presenting cells (APCs) to produce a second population of TILs, wherein the priming first expansion is performed in a container comprising a first gas-permeable surface area, wherein the priming first expansion is performed for first period of about 1 to 7 / 8 days to obtain the second population of TILs, wherein the second population of TILs is greater in number than the first population of TILs;(f) performing a rapid second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and APCs, to produce a third population of TILs, wherein the number of APCs added in the rapid second expansion is at least twice the number of APCs added in step (b), wherein the rapid second expansion is performed for a second period of about 1 to 11 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the rapid second expansion is performed in a container comprising a second gas-permeable surface area;(g) harvesting therapeutic population of TILs obtained from step (f), wherein the transition from step (f) to step (g) occurs without opening the system; and(h) transferring the harvested TIL population from step (1) to an infusion bag, wherein the transfer from step (g) to (h) occurs without opening the system; and(i) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process.

[0087] In some embodiments, the rapid expansion can proceed for 1 day, 2 days, 3 days, 4, days, 5 days, 6 days, 7 days, 8 days, 9 days or 10 days after initiation of the rapid expansion.

[0088] In some embodiments, the present disclosures provide a method of making a cry opreserved population of TILs from a cancer patient, comprising:(a) prior to die patient receives a cancer treatment, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cry opreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) waiting for the patient to exhibit progressive disease on or after the cancer treatment;(d) thawing the cryopreserved tumor fragments or tumor digest and adding the first population of TILs into a closed system;(e) performing a priming first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2, OKT-3, and antigen presenting cells (APCs) to produce a second population of TILs, wherein the priming first expansion is performed in a container comprising a first gas-permeable surface area, wherein the priming first expansion is performed for first period of about 1 to 7 / 8 days to obtain the second population of TILs, wherein the second population of TILs is greater in number than the first population of TILs;(f) performing a rapid second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and APCs, to produce a third population of TILs, wherein the number of APCs added in the rapid second expansion is at least twice the number of APCs added in step (b), wherein the rapid second expansion is performed for a second period of about 1 to 11 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the rapid second expansion is performed in a container comprising a second gas-permeable surface area;(g) harvesting therapeutic population of TILs obtained from step (f), wherein the transition from step (f) to step (g) occurs without opening the system; and(h) transferring the harvested TIL population from step (1) to an infusion bag, wherein the transfer from step (g) to (h) occurs without opening the system; and(i) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process.

[0089] In some embodiments, the patient is naive to any cancer treatment.

[0090] In some embodiments, the patient receives a first-line ICI treatment and / or standard treatment.

[0091] In some embodiments, the patient receives a second-line ICI treatment and / or standard treatment.

[0092] In some embodiments, step (b) comprises flash freezing of the tumor fragments or tumor digest.

[0093] In some embodiments, the flash freezing comprises: i) incubating the tumor fragments or tumor digest in a cry opreservation medium; optionally incubating for about 30 minutes to about 60 minutes at about 2°C to about 8°C in a cry opreservation medium comprising 10% v / v DMSO, andii) freezing the tumor wherein the freezing is flash freezing using the vapor phase of liquid nitrogen.

[0094] In some embodiments, step (b) comprises controlled-rate freezing of the tumor fragments or tumor digest.

[0095] In some embodiments, the controlled-rate freezing comprises: i) adding crvoprcscrvation medium to a closable vessel; ii) pre-cooling the closable vessel in a controlled-rate freezing device; iii) placing the tumor in the closable vessel comprising cryopreservation medium and closing the vessel; iv) incubating the closed vessel comprising the tumor and cryopreservation medium at a temperature of about 2-8C for a time period of about 30 to 60 minutes; and v) slow-freezing the vessel in a controlled-rate freezing device.

[0096] In some embodiments, the cancer patient is naive to any cancer treatment.

[0097] In some embodiments, the patient is naive to TCI treatment, anti-VEGF treatment, or chemotherapeutic treatment.

[0098] In some embodiments, the patient is on maintenance therapy that is interrupted at the time of step (a), and the maintenance therapy is resumed after step (a).

[0099] In some embodiments, the patient receives a first-line ICI treatment and / or standard treatment.

[0100] In some embodiments, the patient receives a second-line ICI treatment and / or standard treatment.

[0101] In some embodiments, the patient is diagnosed with NSCLC.

[0102] In some embodiments, the patient is diagnosed with metastatic stage IV NSCLC.

[0103] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining a first population of TILs from a tumor resected from a subject by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cryopreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) thawing the cry opreserved tumor fragments or tumor digest and adding the the first population of TILs into a closed system;(d) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-11 days to obtain the second population of TILs, and wherein the transition from step (c) to step (d) occurs without opening the system;(e) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-11 days to obtain the third population of TILs, wherein the second expansion is performed in a closed container providing a second gas-permeable surface area, and wherein the transition from step (d) to step (e) occurs without opening the system;(f) harvesting the third population of TILs obtained from step (e), wherein the transition from step (e) to step (f) occurs without opening the system;(g) transferring the harvested third TIL population from step (f to an infusion bag, wherein the transfer from step (f) to (g) occurs without opening the system;(h) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cryoprcscrvation process;

[0104] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cryoprcscrving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) thawing the cryopreserved tumor fragments or tumor digest and adding the first population of TILs into a closed system;(d) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-14 days to obtain the second population of TILs, and wherein the transition from step (c) to step (d) occurs without opening the system;(e) perfonning a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-14 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the second expansion is performed in a closed container providing a second gas-permeable surface area, and wherein the transition from step (d) to step (e) occurs without opening the system;(f) harvesting therapeutic population of TILs obtained from step (e), wherein the transition from step (e) to step (I) occurs without opening the system; and(g) transferring the harvested TIL population from step (f) to an infusion bag, wherein the transfer from step (f) to (g) occurs without opening the system; and(li) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process.

[0105] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining a first population of TILs from a tumor resected from a subject by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cry opreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) thawing the cryopreserved tumor fragments or tumor digest and adding the the first population of TILs into a closed system;(d) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-11 days to obtain the second population of TILs, and wherein the transition from step (c) to step (d) occurs without opening the system;(e) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-11 days to obtain the third population of TILs, wherein the second expansion is performed in a closed container providing a second gas-permeable surface area, and wherein the transition from step (d) to step (e) occurs without opening the system;(f) harvesting the third population of TILs obtained from step (e), wherein the transition from step (e) to step (f) occurs without opening the system;(g) transferring the harvested third TIL population from step (1) to an infusion bag, wherein the transfer from step (f) to (g) occurs without opening the system; and(h) cryoprcscrving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process.

[0106] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from surgical resection, needle biopsy, core biopsy, small biopsy, or other means for obtaining a sample that contains a mixture of tumor and TIL cells from a tumor in the subject or patient,(b) cry opreserving the sample comprising the first population of TILs from step (a) to produce cryopreserved sample;(c) thawing the cryopreserved sample and adding the first population of TILs into a closed system;(d) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-11 days to obtain the second population of TILs, and wherein the transition from step (c) to step (d) occurs without opening the system;(e) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-11 days to obtain the third population of TILs, wherein the second expansion is performed in a closed container providing asecond gas-permeable surface area, and wherein the transition from step (d) to step (e) occurs without opening the system;

[0107] (f) harvesting the third population of TILs obtained from step (e), wherein the transition from step (e) to step (f) occurs without opening the system;(g) transferring the harvested third TIL population from step (1) to an infusion bag, wherein the transfer from step (f) to (g) occurs without opening the system; and(h) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cryoprcscrvation process.

[0108] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, resecting a tumor from the subject or patient, the tumor comprising a first population of TILs, optionally from surgical resection, needle biopsy, core biopsy, small biopsy, or other means for obtaining a sample that contains a mixture of tumor and TIL cells from a tumor;(b) cry opreserving the sample comprising the first population of TILs from step (a) to produce cryopreserved sample;(c) thawing the cry opreserved sample and adding the first population of TILs into a closed system;(d) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-11 days to obtain the second population of TILs and wherein the transition from step (c) to step (d) occurs without opening the system;(e) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-11 days to obtain the third population of TILs, wherein the second expansion is performed in a closed container providing a second gas-permeable surface area, and wherein the transition from step (d) to step (e) occurs without opening the system;(f) harvesting the third population of TILs obtained from step (e), wherein the transition from step (e) to step (f) occurs without opening the system;(g) transferring the harvested third TIL population from step (e) to an infusion bag, wherein the transfer from step (f) to (g) occurs without opening the system; and(h) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process.

[0109] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cry opreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) thawing the cryopreserved tumor fragments or tumor digest and adding the first population of TILs into a closed system;(d) performing a priming first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2, OKT-3, and antigen presenting cells (APCs) to produce a second population of TILs, wherein the priming first expansion is performed in a container comprising a first gas-pcrmcablc surface area, wherein the priming first expansion is performed for first period of about 1 to 7 / 8 days to obtain the second population of TILs, wherein the second population of TILs is greater in number than the first population of TILs;(e) performing a rapid second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and APCs, to produce a third population of TILs, wherein the number of APCs added in the rapid second expansion is at least twice the number of APCs added in step (b), wherein the rapid second expansion is perforated for a second period of about 1 to 11 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the rapid second expansion is performed in a container comprising a second gas-permeable surface area;(f) harvesting therapeutic population of TILs obtained from step (e), wherein the transition from step (e) to step (f) occurs without opening the system; and(g) transferring the harvested TIL population from step (f) to an infusion bag, wherein the transfer from step (f) to (g) occurs without opening the system; and(h) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process.

[0110] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from surgical resection, needle biopsy, core biopsy, small biopsy, or other means for obtaining a sample that contains a mixture of tumor and TIL cells from the subject or patient;(b) cryopreserving the sample comprising the first population of TILs from step (a) to produce cryopreserved sample;(c) thawing the cryopreserved sample and contacting the first population of TILs with a first cell culture medium in a closed system;(d) performing a priming first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2, OKT-3, and antigen presenting cells (APCs) to produce a second population of TILs, wherein the priming first expansion is performed in a container comprising a first gas-permeable surface area, wherein the priming first expansion is performed for first period of about 1 to 7 / 8 days to obtain the second population of TILs, wherein the second population of TILs is greater in number than the first population of TILs;(e) performing a rapid second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and APCs, to produce a third population of TILs, wherein the number of APCs added in the rapid second expansion is at least twice the number of APCs added in step (b), wherein the rapid second expansion is performed for a second period of about 1 to 11 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the rapid second expansion is performed in a container comprising a second gas-permeable surface area;(f) harvesting therapeutic population of TILs obtained from step (f), wherein the transition from step (e) to step (1) occurs without opening the system; and(g) transferring the harvested TIL population from step (g) to an infusion bag, wherein the transfer from step (1) to (g) occurs without opening the system; and(li) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process.

[0111] In some embodiments, the rapid expansion can proceed for 1 day, 2 days, 3 days, 4, days, 5 days, 6 days, 7 days, 8 days, 9 days or 10 days after initiation of the rapid expansion.

[0112] In some embodiments, the IL-2 is present at an initial concentration of between 1000 lU / mL and 6000 lU / mL in the first cell culture medium, when present.

[0113] In some embodiments, the IL-2 is present at an initial concentration of between 1000 lU / mL and 6000 lU / mL and the OKT-3 antibody is present at an initial concentration of about 30 ng / mL in the second wherein the initial expansion, when present, is performed using a gas permeable container.

[0114] In some embodiments, the rapid expansion, when present, is performed using a gas permeable container.

[0115] In some embodiments, the first cell culture medium, when present, further comprises a cytokine selected from the group consisting of IL-4, IL-7, IL-15, IL -21, and combinations thereof.

[0116] In some embodiments, the second cell culture medium, when present, further comprises a cytokine selected from the group consisting of IL-4, IL-7, IL-15, IL-21, and combinations thereof.

[0117] In some embodiments, the harvested population of TILs comprises a therapeutically effective population of TILs, and wherein a therapeutically effective population of TILs comprises from about 2.3x 1010 to about 13.7x 1010 TILs.

[0118] In some embodiments, the initial expansion is performed over a period of 21 days or less.

[0119] In some embodiments, the initial expansion is performed over a period of 7 days or less.

[0120] In some embodiments, the rapid expansion is performed over a period of 7 days or less.

[0121] In some embodiments, the first expansion and the second expansion are each individually performed within a period of 11 days.

[0122] In some embodiments, step of performing the first expansion through the setp of harvesting are performed in about 10 days to about 24 days.

[0123] In some embodiments, step of performing the first expansion through the setp of harvesting are performed in about 10 days to about 22 days.

[0124] In some embodiments, the method further comprising a step of modifying a portion of the TILs at any time prior to the harvesting such that each of the modified TILs comprises an immunomodulatory composition associated with its surface membrane.

[0125] In some embodiments, the step of modifying comprises gene-editing the TILs to effect transfer of at least one gene editing system selected from the group consisting of a TALEN system, a CRISPR system and zinc finger system, into the modified TILs.

[0126] In some embodiments, during the step of modifying the cell membranes of the modified TILs are temporarily disrupted to effect transfer of the at least one gene editing system by using a microfluidic platform or sterile electroporation.

[0127] In some embodiments, the microfluidic platform is a SQZ platform.

[0128] In some embodiments, immunomodulatory composition comprises one or more membrane anchored immunomodulatory fusion proteins each comprising one or more immunomodulatory agents and a cell membrane anchor moiety.

[0129] In some embodiments, the immunomodulatory composition comprises two or more different membrane anchored immunomodulatory fusion proteins, wherein each of the different membrane anchored immunomodulatory fusion proteins each comprises a different immunomodulatory agent.

[0130] In some embodiments, immunomodulatory composition comprises a fusion protein comprising one or more immunomodulatory agents linked to a TIL surface antigen binding domain.

[0131] In some embodiments, immunomodulatory composition comprises a nanoparticle comprising a plurality of immunomodulatory agents.

[0132] In some embodiments, the immunomodulatory agents comprise one or more cytokines.

[0133] In some embodiments, the one or more cytokines comprise IL-2, IL-6, IL-7, IL-9, IL- 12, IL-15, IL-18, IL-21, IL-23, IL-27, IFN gamma, TNFa, IFN alpha, IFN beta, GM-CSF, or GCSF or a variant thereof.

[0134] In some embodiments, the immunomodulatory agents comprise a CD40 agonist.

[0135] In some embodiments, the membrane anchored immunomodulatory fusion protein is according to the formula, from N- to C-terminus: S-IA-L-C, wherein S is a signal peptide, IA is an immunomodulatory agent, L is a linker and C is a cell membrane anchor moiety.

[0136] In some embodiments, the cell membrane anchor moiety comprises a CD8a transmembrane -intracellular domain, a B7-1 transmembrane domain, a B7-2 transmembrane domain, or a CD8a transmembrane domain.

[0137] In some embodiments, the one or more immunomodulatory agents comprise one or more cytokines.

[0138] In some embodiments, the one or more cytokines comprises IL-2, IL-6, IL-7, IL-9, IL-12, IL-15, IL-18, IL-21, IL-23, IL-27, IFN gamma, TNFa, IFN alpha, IFN beta, GM-CSF, or GCSF or a variant thereof.

[0139] In some embodiments, the TIL surface antigen binding domain comprises an antibody variable heavy domain and variable light domain.

[0140] In some embodiments, the TIL surface antigen binding domain comprises an antibody or fragment thereof.

[0141] In some embodiments, the TIL surface antigen binding domain exhibits an affinity for one or more of following TIL surface antigens: CD45, CD4, CD8, CD3, CDlla, CDllb, CDllc, CD18, CD25, CD127, CD19, CD20, CD22, HLA-DR, CD197, CD38, CD27, CD196, CXCR3, CXCR4, CXCR5, CD84, CD229, CCR1, CCR5, CCR4, CCR6, CCR8, CCR10, CD 16, CD56, CD 137. 0X40, or GITR.

[0142] In some embodiments, the nanoparticle is a liposome, a protein nanogel, a nucleotide nanogel, a polymer nanoparticle, or a solid nanoparticle.

[0143] In some embodiments, the nanoparticle is a nanogel.

[0144] In some embodiments, the nanoparticle further comprises an antigen binding domain that binds to one or more of the following antigens: CD45, CDlla (integrin alpha- L), CD 18 (integrin beta-2), CDllb, CDllc, CD25, CD8, or CD4.

[0145] In some embodiments, the modifying comprises introducing a heterologous nucleic acid encoding the fusion protein into the portion of TILs and expressing the fusion protein on the surface of the modified TILs.

[0146] In some embodiments, the heterologous nucleic acid is introduced into the genome of the modified TIL using one or more methods selected from a CRISPR method, a TALE method, a zinc finger method, and a combination thereof.

[0147] In some embodiments, the modifying comprises incubating the fusion protein with the portion of TILs under conditions to permit the binding of the fusion protein to the portion of TILs.

[0148] In some embodiments, the modifying comprises attaching the immunomodulatory composition to the surface of the portion of TILs.

[0149] In some embodiments, the modifying is carried out on TILs from the first expansion, or TILs from the second expansion, or both.

[0150] In some embodiments, the modifying is carried out on TILs from the priming first expansion, or TILs from the rapid second expansion, or both.

[0151] In some embodiments, the modifying is carried out after the first expansion and before the second expansion.

[0152] In some embodiments, the modifying is carried out after the priming first expansion and before the rapid second expansion, or both.

[0153] In some embodiments, the modifying is carried out after the second expansion.

[0154] In some embodiments, the modifying is carried out after the rapid second expansion.

[0155] In some embodiments, the modifying is carried out after the harvesting.

[0156] In some embodiments, the modified TILs further comprise a genetic modification that causes expression of one or more immune checkpoint genes to be silenced or reduced in at least a portion of the therapeutic population of TILs.

[0157] In some embodiments, said one or more immune checkpoint genes is / are selected from the group comprising PD-1, CTLA-4, LAG-3, HAVCR2 (TIM-3), Cish, TGFfy PKA, CBL-B, PPP2CA, PPP2CB, PTPN6, PTPN22, PDCD1, BTLA, CD 160, TIGIT, TET2, CD96, CRT AM, LAIR1, SIGLEC7, SIGLEC9, CD244, TNFRSF10B, TNFRSF10A, CASP8, C ASP 10, CASP3, CASP6, CASP7, FADD, FAS, SMAD2, SMAD3, SMAD4, SMAD10, SKI, SKIL, TGIF1, IL10RA, IL10RB, HMOX2, IL6R, IL6ST, EIF2AK4, CSK, PAG1, SIT1, FOXP3, PRDM1, BATF, GUCY1A2, GUCY1A3, GUCY1B2, GUCY1B3, TOX, S0CS1, ANKRD11, and BCOR.

[0158] In some embodiments, said one or more immune checkpoint genes is / are selected from the group comprising PD-1, CTLA-4, LAG-3, HAVCR2 (TIM-3), Cish, TGF(3, and PKA.

[0159] In some embodiments, the modified TILs further comprises a genetic modification that causes expression of one or more immune checkpoint genes to be enhanced in at least a portion of the therapeutic population of TILs, the immune checkpoint gene(s) being selected from the group comprising CCR2, CCR4, CCR5, CXCR2, CXCR3, CX3CR1, IL-2, IL-4, IL-7, IL-10, IL-15, IL-21, the NOTCH 1 / 2 intracellular domain (ICD), and / or the NOTCH ligand mDLLl.

[0160] In some embodiments, the genetic modification is produced using a programmable nuclease that mediates the generation of a double-strand or single-strand break at said one or more immune checkpoint genes.

[0161] In some embodiments, the genetic modification is produced using one or more methods selected from a CRISPR method, a TALE method, a zinc finger method, and a combination thereof.

[0162] In some embodiments, the modified TILs are modified to transiently express the immunomodulatory composition on the cell surface.

[0163] In some embodiments, the immunomodulatory composition comprises one or more membrane anchored immunomodulatory fusion proteins, wherein each fusion protein comprises one or more immunomodulatory agents and a cell membrane anchor moiety.

[0164] In some embodiments, the modified TILs are modified by transfecting the TILs with a nucleic acid encoding the fusion protein.

[0165] In some embodiments, the nucleic acid is an RNA.

[0166] In some embodiments, the RNA is a mRNA.

[0167] In some embodiments, the TILs are transfected with the mRNA by a SQZ platform or electroporation.

[0168] In some embodiments, the TILs are transfected with the mRNA by a SQZ platform or electroporation after the first expansion and before the second expansion.

[0169] In some embodiments, the TILs are transfected with the mRNA by a SQZ platform or electroporation before the first expansion.

[0170] In some embodiments, the modified TILs are transfected with the nucleic acid encoding the fusion protein using a microfluidic device to temporarily disrupt the cell membranes of the TILs, thereby allowing transfection of the nucleic acid.

[0171] In some embodiments, the method further comprises activating the TILs by incubation with an anti-CD3 agonist before transfecting the TILs with the mRNA.

[0172] In some embodiments, the anti-CD3 agonist is OKT-3.

[0173] In some embodiments, the TILs are activated by incubating the TILs with the anti-CD3 agonist for about 1 to 3 days before transfecting the TILs with the mRNA.

[0174] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) cryopreserving the tumor tissue comprising the first population of TILs from step (a) to produce a cryopreserved tumor tissue;(c) thawing the cryoprcscrvcd tumor tissue and culturing the first population of TILs in a first cell culture medium comprising IL-2 for about 3-9 days to produce a second population of TILs;(d) activating the second population of TILs using anti-CD3 agonist beads or antibodies, or anti-CD3 agonist and anti-CD28 agonist beads or antibodies, for 1-7 days, to produce a third population of TILs;(e) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs; and(f) culturing the fourth population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 5-15 days, to produce an expanded number of TILs.

[0175] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) digesting in an enzyme media the tumor tissue to produce a tumor digest;(c) cry opreserving the tumor digest comprising the first population of TILs from step (a) to produce a cryopreserved tumor digest;(d) thawing the cryoprcscrvcd tumor digest and culturing the first population of TILs in a first cell culture medium comprising IL-2 for about 3-9 days to produce a second population of TILs;(e) activating the second population of TILs using anti-CD3 agonist beads or antibodies, or anti-CD3 agonist and anti-CD28 agonist beads or antibodies, for 1-7 days, to produce a third population of TILs;(f) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs; and(g) culturing the fourth population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 5-15 days, to produce an expanded number of TILs.

[0176] In some embodiments, the enzymatic media comprises a DNase.

[0177] In some embodiments, the enzymatic media comprises a collagenase.

[0178] In some embodiments, the enzymatic media comprises a neutral protease.

[0179] In some embodiments, the enzymatic media comprises a hy aluronidase.

[0180] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) cry opreserving the tumor tissue comprising the first population of TILs from step (a) to produce a cryopreserved tumor tissue;(c) thawing the cryopreserved tumor tissue and culturing the first population of TILs in a first cell culture medium comprising IL-2 for about 3-9 days to produce a second population of TILs;(d) activating the second population of TILs using anti-CD3 agonist beads or antibodies, or anti-CD3 agonist and anti-CD28 agonist beads or antibodies, for 1-7 days, to produce a third population of TILs;(e) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs;(f) culturing the fourth population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 1-7 days, to produce a culture of a fifth population of TILs; and(g) splitting the culture of the fifth population of TILs into a plurality of subcultures, culturing each of the plurality of subcultures in a third cell culture medium comprising IL-2 for about 3-6 days, and combining the plurality of subcultures to provide an expanded number of TILs.

[0181] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) digesting in an enzyme media the tumor tissue to produce a tumor digest;(c) cry opreserving the tumor digest comprising the first population of TILs from step (a) to produce a cryoprcscrvcd tumor digest;(d) thawing the cryoprcscrvcd tumor digest and culturing the first population of TILs in a first cell culture medium comprising IL-2 for about 3-9 days to produce a second population of TILs;(e) activating the second population of TILs using anti-CD3 agonist beads or antibodies, or anti-CD3 agonist and anti-CD28 agonist beads or antibodies, for 1-7 days, to produce a third population of TILs;(f) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs;(g) culturing the fourth population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 1-7 days, to produce a culture of a fifth population of TILs; and(h) splitting the culture of the fifth population of TILs into a plurality of subcultures, culturing each of the plurality of subcultures in a third cell culture medium comprising IL-2 for about 3-6 days, and combining the plurality of subcultures to provide an expanded number of TILs.

[0182] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) cry opreserving the tumor tissue comprising the first population of TILs from step (a) to produce a cryopreserved tumor tissue;(c) thawing the cryopreserved tumor tissue and culturing the first population of TILs in a first cell culture medium comprising IL-2 and OKT-3 for about 3-9 days to produce a second population of TILs;(d) gene-editing at least a portion of the second population of TILs, to produce a third population of TILs; and(e) culturing the third population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 5-15 days, to produce an expanded number of TILs.

[0183] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) digesting in an enzyme media the tumor tissue to produce a tumor digest;(c) cry opreserving the tumor digest comprising the first population of TILs from step (a) to produce a cryopreserved tumor digest;(d) thawing the cryopreserved tumor digest and culturing the first population of TILs in a first cell culture medium comprising IL-2 and OKT-3 for about 3-9 days to produce a second population of TILs;(e) gene-editing at least a portion of the second population of TILs, to produce a third population of TILs; and(f) culturing the third population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 5-15 days, to produce an expanded number of TILs.

[0184] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) cry opreserving the tumor tissue comprising the first population of TILs from step (a) to produce a cryopreserved tumor tissue;(c) thawing the cryopreserved tumor tissue and culturing the first population of TILs in a first cell culture medium comprising IL-2 and OKT-3 for about 3-9 days to produce a second population of TILs;(d) gene-editing at least a portion of the second population of TILs, to produce a third population of TILs;(e) culturing the third population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 1-7 days, to produce a culture of a fourth population of TILs; and(I) splitting the culture of the fourth population of TILs into a plurality of subcultures, culturing each of the plurality of subcultures in a third cell culture medium comprising IL-2 for about 3-7 days, and combining the plurality of subcultures to provide a fifth population of TILs comprising an expanded number of TILs.

[0185] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) digesting in an enzyme media the tumor tissue to produce a tumor digest;(c) cry opreserving the tumor digest comprising the first population of TILs from step (a) to produce a cryopreserved tumor digest;(d) thawing the cryopreserved tumor digest and culturing the first population of TILs in a first cell culture medium comprising IL-2 and OKT-3 for about 3-9 days to produce a second population of TILs;(e) gene-editing at least a portion of the second population of TILs, to produce a third population of TILs;(I) culturing the third population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 1-7 days, to produce a culture of a fourth population of TILs; and(g) splitting the culture of the fourth population of TILs into a plurality of subcultures, culturing each of the plurality of subcultures in a third cell culture medium comprising IL-2 for about 3-7 days, and combining the plurality of subcultures to provide a fifth population of TILs comprising an expanded number of TILs.

[0186] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) cry opreserving the tumor tissue comprising the first population of TILs from step (a) to produce a cryopreserved tumor tissue;(c) thawing the cryoprcscrvcd tumor tissue and culturing the first population of TILs in a first cell culture medium comprising IL-2 for about 3 days to produce a second population of TILs;(d) culturing the second population of TILs in a second cell culture medium comprising IL-2 and OKT-3 for 2-4 days to produce a third population of TILs;(e) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs; and(f) culturing the fourth population of TILs in a third cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 5-15 days, to produce an expanded number of TILs.

[0187] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) digesting in an enzyme media the tumor tissue to produce a tumor digest;(c) cry opreserving the tumor digest comprising the first population of TILs from step (a) to produce a cryopreserved tumor digest;(d) thawing the cryoprcscrvcd tumor digest and culturing the first population of TILs in a first cell culture medium comprising IL-2 for about 3 days to produce a second population of TILs;(e) culturing the second population of TILs in a second cell culture medium comprising IL-2 and OKT-3 for 2-4 days to produce a third population of TILs;(I) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs; and(g) culturing the fourth population of TILs in a third cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 5-15 days, to produce an expanded number of TILs.

[0188] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) cry opreserving the tumor tissue comprising the first population of TILs from step (a) to produce a cryopreserved tumor tissue;(c) thawing the cryopreserved tumor tissue and culturing the first population of TILs in a first cell culture medium comprising IL-2 for about 3 days to produce a second population of TILs;(d) culturing the second population of TILs in a second cell culture medium comprising IL-2 and OKT-3 for 2-4 days to produce a third population of TILs;(e) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs;(!) culturing the third population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 1-7 days, to produce a culture of a fourth population of TILs; and(g) splitting the culture of the fourth population of TILs into a plurality of subcultures, culturing each of the plurality of subcultures in a third cell culture medium comprising IL-2 for about 3-7 days, and combining the plurality of subcultures to provide a fifth population of TILs comprising an expanded number of TILs.

[0189] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) digesting in an enzyme media the tumor tissue to produce a tumor digest;(c) cry opreserving the tumor digest comprising the first population of TILs from step (a) to produce a cryopreserved tumor digest;(d) thawing the cryopreserved tumor digest and culturing the first population of TILs in a first cell culture medium comprising IL-2 for about 3 days to produce a second population of TILs;(e) culturing the second population of TILs in a second cell culture medium comprising IL-2 and OKT-3 for 2-4 days to produce a third population of TILs;(f) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs;(g) culturing the fourth population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 1-7 days, to produce a culture of a fifth population of TILs; and(h) splitting the culture of the fifth population of TILs into a plurality of subcultures, culturing each of the plurality of subcultures in a third cell culture medium comprising IL-2 for about 3-6 days, and combining the plurality of subcultures to provide a fifth population of TILs comprising an expanded number of TILs.

[0190] In some embodiments, the present disclosures provide a method of making a cryoprcscrvcd population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) cry opreserving the tumor tissue comprising the first population of TILs from step (a) to produce a cryopreserved tumor tissue;(c) thawing the cryopreserved tumor tissue and performing an initial expansion (or priming first expansion) of the first population of TILs in a first cell culture medium to obtain a second population of TILs, wherein the first cell culture medium comprises IL-2, optionally OKT-3, and optionally antigen presenting cells (APCs), wherein the priming first expansion occurs for a period of about 3 to 8 days;(d) activating the second population of TILs using anti-CD3 agonist beads or antibodies, or anti-CD3 agonist and anti-CD28 agonist beads or antibodies, for 1-6 days, to produce a third population of TILs;(e) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs;(f) performing a rapid second expansion of the fourth population of TILs in a second cell culture medium to obtain an expanded number of TILs, wherein the second cell culture medium comprises IL-2, OKT-3, and APCs; and wherein the rapid expansion is performed over a period of 14 days or less, optionally the rapid second expansion can proceed for about 1 day, 2 days, 3 days, 4, days, 5 days, 6 days, 7 days, 8 days, 9 days or 10 days after initiation of the rapid second expansion.

[0191] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) cry opreserving the tumor tissue comprising the first population of TILs from step (a) to produce a cryopreserved tumor tissue;(c) subsequent to the cancer progression, performing an initial expansion (or priming first expansion) of the first population of TILs in a first cell culture medium to obtain a second population of TILs, wherein the first cell culture medium comprises IL-2, optionally OKT-3, and optionally antigen presenting cells (APCs), wherein the priming first expansion occurs for a period of about 3 to 8 days;(d) gene-editing at least a portion of the second population of TILs, to produce a third population of TILs; and(e) performing a rapid second expansion of the third population of TILs in a second cell culture medium to obtain an expanded number of TILs, wherein the second cell culture medium comprises IL-2, OKT-3, and APCs; and wherein the rapid expansion is performed over a period of 14 days or less, optionally the rapid second expansion can proceed for about 1 day, 2 days, 3 days, 4, days, 5 days, 6 days, 7 days, 8 days, 9 days or 10 days after initiation of the rapid second expansion.

[0192] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) digesting in an enzyme media the tumor fragments to produce a tumor digest;(c) cry opreserving the tumor digest comprising the first population of TILs from step (a) to produce a cryopreserved tumor digest;(d) thawing the crvoprcscrvcd tumor digest and performing an initial expansion (or priming first expansion) of the first population of TILs in a first cell culture medium to obtain a second population of TILs, wherein the first cell culture medium comprises IL-2, optionally OKT-3, and optionally antigen presenting cells (APCs), where the priming first expansion occurs for a period of about 1 to 8 days;(e) activating the second population of TILs using anti-CD3 agonist beads or antibodies, or anti-CD3 agonist and anti-CD28 agonist beads or antibodies, for 1-6 days, to produce a third population of TILs;(f) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs;(g) performing a rapid second expansion of the fourth population of TILs in a second cell culture medium to obtain an expanded number of TILs, wherein the second cell culture medium comprises IL-2, OKT-3, and APCs; and wherein the rapid expansion is performed over a period of 14 days or less, optionally the rapid second expansion can proceed for about 1 day, 2 days, 3 days, 4, days, 5 days, 6 days, 7 days, 8 days, 9 days or 10 days after initiation of the rapid second expansion.

[0193] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) digesting in an enzyme media the tumor fragments to produce a tumor digest;(c) cry opreserving the tumor digest comprising the first population of TILs from step (a) to produce a cryopreserved tumor digest;(d) thawing the crvoprcscrvcd tumor digest and performing an initial expansion (or priming first expansion) of the first population of TILs in a first cell culture medium to obtain a second population of TILs, wherein the first cell culture medium comprises IL-2, optionally OKT-3, and optionally antigen presenting cells (APCs), where the priming first expansion occurs for a period of about 1 to 8 days;(e) gene-editing at least a portion of the second population of TILs, to produce a third population of TILs; and(f) performing a rapid second expansion of the third population of TILs in a second cell culture medium to obtain an expanded number of TILs, wherein the second cell culture medium comprisesIL-2, OKT-3, and APCs; and wherein the rapid expansion is performed over a period of 14 days or less, optionally the rapid second expansion can proceed for about 1 day, 2 days, 3 days, 4, days, 5 days, 6 days, 7 days, 8 days, 9 days or 10 days after initiation of the rapid second expansion.

[0194] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a sample of tumor tissue produced by surgical resection, needle biopsy, core biopsy, small biopsy, or other means for obtaining tumor tissue from a patient or subject;(b) cry opreserving the tumor tissue comprising the first population of TILs from step (a) to produce a cryopreserved tumor tissue;(c) thawing the cry opreserved tumor tissue and adding the tumor tissue into a closed system and performing a first expansion by culturing the first population of TILs in a first cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-9 days to obtain the second population of TILs;(d) activating the second population of TILs using anti-CD3 agonist beads or antibodies, or CD3 agonist and CD28 agonist beads or antibodies, for 1-7 days, to produce a third population of TILs;(e) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs;(f) performing a second expansion by culturing the fourth population of TILs in a second cell culture medium comprising IL-2, OKT-3, and antigen presenting cells (APCs), to produce a fifth population of TILs, wherein the second expansion is performed for about 5-15 days to obtain the fifth population of TILs, wherein the second expansion is performed in a closed container providing a second gas- permeable surface area, wherein the fifth population of TILs is a therapeutic population of TILs; and(g) harvesting the therapeutic population of TILs obtained from step (f), wherein each of steps (c) to (g) is performed in a closed, sterile system, and wherein the transition from step (c) to step (d), the transition from step (d) to step (e), the transition from step (e) to step (f) and / or die transition from step (!) to step (g) occurs without opening the system.

[0195] In some embodiments, the present disclosures provide a method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintenance therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a sample of tumor tissue produced by surgical resection, needle biopsy, core biopsy, small biopsy, or other means for obtaining tumor tissue from a patient or subject;(b) digesting the sample of tumor tissue in an enzy matic media to produce a tumor digest;(c) cry opreserving the tumor digest comprising the first population of TILs to produce a cryopreserved tumor digest;(d) thawing the cryopreserved tumor digest and adding the tumor digest into a closed system and performing a first expansion by culturing the first population of TILs in a first cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-9 days to obtain the second population of TILs;(d) activating the second population of TILs using anti-CD3 agonist beads or antibodies, or CD3 agonist and CD28 agonist beads or antibodies, for 1-7 days, to produce a third population of TILs;(e) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs;(f) performing a second expansion by culturing the fourth population of TILs in a second cell culture medium comprising IL-2, OKT-3, and antigen presenting cells (APCs), to produce a fifth population of TILs, wherein the second expansion is performed for about 5-15 days to obtain the fifth population of TILs, wherein the second expansion is performed in a closed container providing a second gas- permeable surface area, wherein the fifth population of TILs is a therapeutic population of TILs; and(g) harvesting the therapeutic population of TILs obtained from step (f), wherein each of steps (d) to (g) is performed in a closed, sterile system, and wherein the transition from step (d) to step (e), the transition from step (e) to step (f), and / or the transition from step (f) to step (g) occurs without opening the system.

[0196] In some embodiments, the rapid second expansion is performed by culturing the third or fourth population of TILs in the second cell culture medium for a first period of about 1-7 days, at the end of the first period the culture is split into a plurality of subcultures, each of the plurality of subcultures is cultured in a third culture medium comprising IL-2 for a second period of about 3-6 days, and at the end of the second period the plurality of subcultures are combined to provide the expanded number of TILs.

[0197] In some embodiments, the step of gene-editing at least a portion of the second or third population of TILs comprises performing a sterile electroporation step or a SQZ microfluidicmembrane disruption step on the second or third population of TILs, wherein the sterile electroporation step or the SQZ microfluidic membrane disruption step mediates the transfer of at least two gene editors.

[0198] In some embodiments, the electroporation step or the SQZ microfluidic membrane disruption step consists of a single event that mediates the transfer of the at least two gene editors.

[0199] In some embodiments, in the electroporation step or the SQZ microfluidic membrane disruption step each of the at least two gene editors is transferred individually by an event independently of the transfer of any other gene editor.

[0200] In some embodiments, the electroporation step further comprises a rest period after each event.

[0201] In some embodiments, the electroporation step or the SQZ microfluidic membrane disruption step comprises a first event that mediates the transfer of a first gene editor for modulating expression of a first protein, a first rest period, a second event that mediates the transfer of a second gene editor for modulating expression of a second protein, and a second rest period, wherein the first and second rest periods are the same or different.

[0202] In some embodiments, the first and second rest periods comprise incubating the third or fourth population of TILs in a cell culture medium comprising IL-2.

[0203] In some embodiments, the first and second rest periods comprise incubating the third or fourth population of TILs at about 30-40 °C with about 5% CO2.

[0204] In some embodiments, the first and second rest periods are independently about 10 hours to 5 days.

[0205] In some embodiments, the first and second rest periods are independently about 10 hours to 3 days.

[0206] In some embodiments, the first rest period is about 1 to 3 days.

[0207] In some embodiments, the first rest period is about 3 days.

[0208] In some embodiments, the second rest period is about 10 hours to 1 day.

[0209] In some embodiments, the second rest period is about 12 hours to 24 hours.

[0210] In some embodiments, the second rest period is about 15 hours to about 18 hours.

[0211] In some embodiments, the second rest period comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2 for about 15 hours to 23 hours at about 30°C.

[0212] In some embodiments, the second rest period comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2 for about for about one hour at 37°C followed by about 15 hours to 23 hours at about 30°C.

[0213] In some embodiments, the second rest period comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2 for about for about one hour at 37°C followed by about 15 hours to 22 hours at about 30°C.

[0214] In some embodiments, the first rest period is about 3 days and the second rest period is about 10 to 16 hours.

[0215] In some embodiments, the at least two gene editors comprise a first gene editor comprising a first TALE nuclease system for modulating expression of a first protein and a second gene editor comprising a second TALE nuclease system for modulating expression of a second protein.

[0216] In some embodiments, the electroporation step or the SQZ microfluidic membrane disruption step comprises a first event that mediates the transfer of the first TALE nuclease system, a first rest period, a second event that mediates the transfer of the second TALE nuclease system, and a second rest period, wherein the first and second rest periods are the same or different.

[0217] In some embodiments, the first and second rest periods comprise incubating the third or fourth population of TILs in a cell culture medium comprising IL-2.

[0218] In some embodiments, the first and second rest periods comprise incubating the third or fourth population of TILs at about 30-40 °C with about 5% CO2.

[0219] In some embodiments, the first and second rest periods are independently about 10 hours to 5 days.

[0220] In some embodiments, the first and second rest periods are independently about 10 hours to 3 days.

[0221] In some embodiments, the first rest period is about 1 to 3 days.

[0222] In some embodiments, the first rest period is about 3 days.

[0223] In some embodiments, the second rest period is about 10 hours to 1 day.

[0224] In some embodiments, the second rest period is about 12 hours to 24 hours.

[0225] In some embodiments, the second rest period is about 15 hours to about 18 hours.

[0226] In some embodiments, the second rest period comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2 for about 15 hours to 23 hours at about 30°C.

[0227] In some embodiments, the second rest period comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2 for about for about one hour at 37°C followed by about 15 hours to 23 hours at about 30°C.

[0228] In some embodiments, the second rest period comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2 for about for about one hour at 37°C followed by about 15 hours to 22 hours at about 30°C.

[0229] In some embodiments, the first rest period is about 3 days and the second rest period is about 10 to 16 hours.

[0230] In some embodiments, the step of gene-editing at least a portion of the second or third population of TILs comprises performing a sterile electroporation step or a SQZ microfluidic membrane disruption step on the second or third population of TILs, wherein the sterile electroporation step or the SQZ microfluidic membrane disruption step mediates the transfer of at least one gene editor.

[0231] In some embodiments, the gene editor is a TALE nuclease system for modulating the expression of at least one protein.

[0232] In some embodiments, the TALE nuclease system modulates expression of PD-1, CTLA-4, TIGIT, CBL-B and / or LAG-3.

[0233] In some embodiments, the gene editor comprises a TALE nuclease system that modulates expression of PD-1 and CTLA-4.

[0234] In some embodiments, the gene editor comprises a TALE nuclease system that modulates expression of PD-1 and LAG-3.

[0235] In some embodiments, the gene editor comprises a TALE nuclease system that modulates expression of PD-1 and CISH.

[0236] In some embodiments, the gene editor comprises a TALE nuclease system that modulates expression of PD-1 and CBL-B.

[0237] In some embodiments, the gene editor comprises a TALE nuclease system that modulates expression ofPD-1 and TIGIT.

[0238] In some embodiments, the gene editor comprises a TALE nuclease system that modulates expression of CTLA-4 and LAG-3.

[0239] In some embodiments, the gene editor comprises a TALE nuclease system that modulates expression of CTLA-4 and CISH.

[0240] In some embodiments, the gene editor comprises a TALE nuclease system that modulates expression of CTLA-4 and CBL-B.

[0241] In some embodiments, the gene editor comprises a TALE nuclease system that modulates expression of CISH and CBL-B.

[0242] In some embodiments, the gene editor comprises a TALE nuclease system that modulates expression of CISH and TIGIT.

[0243] In some embodiments, the gene editor comprises a TALE nuclease system that modulates expression ofPD-1.

[0244] In some embodiments, the gene editor comprises a TALE nuclease system that modulates expression of CTLA-4.

[0245] In some embodiments, the gene editor comprises a TALE nuclease system that modulates expression of LAG-3.

[0246] In some embodiments, the gene editor comprises a TALE nuclease system that modulates expression of CISH.

[0247] In some embodiments, the gene editor comprises a TALE nuclease system that modulates expression of CBL-B.

[0248] In some embodiments, the gene editor comprises a TALE nuclease system that modulates expression of TIGIT.

[0249] In some embodiments, the method further comprises the step of resting the third or fourth population of TILs after the gene-editing step and before the step of culturing the third or fourth population of TILs.

[0250] In some embodiments, the resting step comprises incubating the third or fourth population of TILs at about 30-40 °C with about 5% CO2.

[0251] In some embodiments, the method further comprises the step of resting the third or fourth population of TILs for about one day after the gene-editing step and before the step of culturing the third or fourth population of TILs.

[0252] The method of any of claims 352-369, wherein the method further comprises the step of resting the third or fourth population of TILs for about 12 hours to 24 hours after the gene-editing step and before the step of culturing the third or fourth population of TILs.

[0253] In some embodiments, the step of resting the third or fourth population of TILs comprises resting the third or fourth population of TILs for about 15 hours to 18 hours.

[0254] In some embodiments, the step of resting the third or fourth population of TILs comprises resting the third or fourth population of TILs for about 15 hours.

[0255] In some embodiments, the step of resting the third or fourth population of TILs comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2.

[0256] In some embodiments, the step of resting the third or fourth population of TILs comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2 for about 15 hours to 23 hours at about 30°C.

[0257] In some embodiments, the step of resting third or fourth population of TILs comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2 for about one hour at 37°C followed by about 15 hours to 23 hours at about 30°C.

[0258] In some embodiments, the step of resting the third or fourth population of TILs comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2 for about one hour at 37°C followed by about 15 hours to 22 hours at about 30°C.

[0259] In some embodiments, the first protein and the second protein are independently selected from the group consisting of PD-1, CTLA-4, LAG-3, CISH, TIGIT and CBL-B, with the proviso that the first protein and the second protein are different.

[0260] In some embodiments, the first protein and the second protein are selected from the group consisting of PD-1 and CTLA-4.

[0261] In some embodiments, the first protein and the second protein are selected from the group consisting of PD-1 and LAG-3.

[0262] In some embodiments, the first protein and the second protein are selected from the group consisting of PD-1 and CISH.

[0263] In some embodiments, the first protein and the second protein are selected from the group consisting of PD-1 and CBL-B.

[0264] In some embodiments, the first protein and the second protein are selected from the group consisting of PD-1 and TIGIT.

[0265] In some embodiments, the first protein and the second protein are selected from the group consisting of CTLA-4 and LAG-3.

[0266] In some embodiments, the first protein and the second protein are selected from the group consisting of CTLA-4 and CISH.

[0267] In some embodiments, the first protein and the second protein are selected from the group consisting of CTLA-4 and CBL-B.

[0268] In some embodiments, the first protein and the second protein are selected from the group consisting of LAG-3 and CISH.

[0269] In some embodiments, the first protein and the second protein are selected from the group consisting of LAG-3 and CBL-B.

[0270] In some embodiments, the first protein and the second protein are selected from the group consisting of CISH and CBL-B.

[0271] In some embodiments, the first protein is PD-1 and the second protein is CTLA-4.

[0272] In some embodiments, the first protein is CTLA-4 and the second protein is PD-1.

[0273] In some embodiments, the first protein is PD-1 and the second protein is LAG-3.

[0274] In some embodiments, the first protein is LAG-3 and the second protein is PD-1.

[0275] In some embodiments, the first protein is PD-1 and the second protein is CISH.

[0276] In some embodiments, the first protein is CISH and the second protein is PD-1.

[0277] In some embodiments, the first protein is PD-1 and the second protein is CBL-B.

[0278] In some embodiments, the first protein is CBL-B and the second protein is PD-1.

[0279] In some embodiments, the first protein is PD-1 and the second protein is TIGIT.

[0280] In some embodiments, the first protein is TIGIT and the second protein is PD-1.

[0281] In some embodiments, the first protein is CTLA-4 and the second protein is LAG-3.

[0282] In some embodiments, the first protein is LAG-3 and the second protein is CTLA-4.

[0283] In some embodiments, the first protein is CTLA-4 and the second protein is CISH.

[0284] In some embodiments, the first protein is CISH and the second protein is CTLA-4.

[0285] In some embodiments, the first protein is CTLA-4 and the second protein is CBL-B.

[0286] In some embodiments, the first protein is CBL-B and the second protein is CTLA-4.

[0287] In some embodiments, the first protein is LAG-3 and the second protein is CISH.

[0288] In some embodiments, the first protein is CISH and the second protein is LAG-3.

[0289] In some embodiments, the first protein is LAG-3 and the second protein is CBL-B.

[0290] In some embodiments, the first protein is CBL-B and the second protein is LAG-3.

[0291] In some embodiments, the first protein is CISH and the second protein is CBL-B.

[0292] In some embodiments, the first protein is CBL-B and the second protein is CISH.

[0293] In some embodiments, the first protein or the second protein is PD-1.

[0294] In some embodiments, the first protein or the second protein is CTLA-4.

[0295] In some embodiments, the first protein or the second protein is LAG-3.

[0296] In some embodiments, the first protein or the second protein is CISH.

[0297] In some embodiments, the first protein or the second protein is CBL-B.

[0298] In some embodiments, the first protein or the second protein is TIGIT.

[0299] In some embodiments, the first gene editor downregulates expression of the first protein and the second gene editor downregulates expression of the second protein.

[0300] In some embodiments, the expanded number of TILs comprises a therapeutic population of TILs.

[0301] In some embodiments, the antigen presenting cells (APCs) are PBMCs.

[0302] In some embodiments, the PBMCs are irradiated and allogeneic.

[0303] In some embodiments, the antigen-presenting cells are artificial antigen-presenting cells.

[0304] In some embodiments, the IL-2 concentration is about 10,000 lU / mL to about 5,000 lU / mL.

[0305] In some embodiments, the first cell culture medium and / or the second cell culture medium further comprises a 4- IBB agonist and / or an 0X40 agonist.

[0306] In some embodiments, the patient is naive to an ICI treatment, an anti-VEGF treatment, a chemotherapeutic treatment, or combinations thereof.

[0307] In some embodiments, the patient is naive to any cancer treatment.

[0308] In some embodiments, the patient receives a first-line ICI treatment and / or standard treatment.

[0309] In some embodiments, the patient receives a second-line ICI treatment and / or standard treatment.

[0310] In some embodiments, step of thawing and the subsequent steps are performed after the patient receives the cancer treatment or resumes the maintenance therapy and exhibits progressive disease.

[0311] In some embodiments, the patient is in a washout period at initiation of step (a).

[0312] In some embodiments, the present disclosures provide a method of treating a cancer patient by administering a population of tumor infiltrating lymphocytes (TILs) to the cancer patient in need thereof, wherein the cancer patient has received at least one prior therapy and has exhibited cancer progression on or after the at least one prior therapy, wherein obtaining the population of the TILs comprises multilesional sampling, wherein the population of TILs was made from a tumor sample harvested before the cancer patient receives the at least one prior therapy; and wherein the population of TILs is administered to the subject or patient after the cancer patient has exhibited cancer progression on or after the at least one prior therapy.

[0313] In some embodiments, the present disclosures provide a method of treating a cancer patient by administering a population of tumor infiltrating lymphocytes (TILs) to the cancer patient in need thereof, wherein the cancer patient has received at least one prior therapy and has exhibited cancer progression on or after the at least one prior therapy, wherein obtaining the population of the TILs comprises multilesional sampling, wherein the population of TILs was made before the cancer patient receives the at least one prior therapy and wherein the population of TILs is administered to the subject or patient after the cancer patient has exhibited cancer progression on or after the at least one prior therapy.

[0314] In some embodiments, at the time of harvesting the tumor sample, the cancer patient is naive to any cancer treatment.

[0315] In some embodiments, at the lime of harvesting the tumor sample, the patient is naive to ICI treatment, anti-VEGF treatment, or chemotherapeutic treatment.

[0316] In some embodiments, at the time of harvesting the tumor sample, the patient is on maintenance therapy that is interrupted at the time of step (a), and the maintenance therapy is resumed after cry opreserving.

[0317] In some embodiments, at the time of harvesting the tumor sample, the patient is in a washout period at initiation of step of cry opreserving.

[0318] In some embodiments, the patient receives or is going to receive a first-line ICI treatment and / or standard treatment.

[0319] In some embodiments, the patient receives or is going to receive a second-line ICI treatment and / or standard treatment.

[0320] In some embodiments, the method further comprising administering an ICI to the cancer patient.

[0321] In some embodiments, the ICI is an inhibitor of a gene selected from the group consisting of PD-1, CTLA-4, LAG-3, HAVCR2 (TIM-3), Cish, TGF0, PKA, CBL-B, PPP2CA, PPP2CB, PTPN6, PTPN22, PDCD1, BTLA, CD 160, TIGIT, TET2, BAFF (BR3), CD96, CRT AM, LAIR1, SIGLEC7, SIGLEC9, CD244, TNFRSF10B, TNFRSF10A, CASP8, C ASP 10, CASP3, CASP6, CASP7, FADD, FAS, SMAD2, SMAD3, SMAD4, SMAD10, SKI, SKIL, TGIF1, IL10RA, IL10RB, HMOX2, IL6R, IL6ST, EIF2AK4, CSK, PAG1, SIT1, FOXP3, PRDM1, BATF, GUCY1A2, GUCY1A3, GUCY1B2, GUCY1B3, TOX, S0CS1, ANKRD11, and BCOR.

[0322] The method of any one of Claims 1 to 76, further comprising the step of treating the patient with a non-myeloablative lymphodepletion regimen prior to administering the population of TILs to the patient.

[0323] The method of claim 77, wherein the non-myeloablative lymphodepletion regimen comprises the steps of administration of cyclophosphamide at a dose of 60 mg / m2 / day for two days followed by administration of fludarabine at a dose of 25 mg / m2 / day for five days.

[0324] The method of Claim 77, wherein the non-myeloablative lymphodepletion regimen comprises the steps of administration of cyclophosphamide at a dose of 60 mg / m2 / day andfludarabine at a dose of 25 mg / m2 / day for two days followed by administration of fludarabine at a dose of 25 mg / m2 / day for three days.

[0325] The method of Claim 79 to 79, wherein the cyclophosphamide is administered with mesna.

[0326] The method of any one of Claims 1 to 80, further comprising the step of treating the patient with an IL-2 regimen starling on the day after administration of the population of TILs to the patient.

[0327] The method of any one of Claims 1 to 80, further comprising the step of treating the patient with an IL-2 regimen starting on the same day as administration of the population of TILs to the patient.

[0328] The method of any one of Claims 1 to 82, wherein the IL-2 regimen is a high-doseIL-2 regimen comprising 600,000 or 720,000 lU / kg of aldesleukin, or a biosimilar or variant thereof, administered as a 15-minute bolus intravenous infusion every eight hours until tolerance.

[0329] An expanded number of tumor infiltrating lymphocytes (TILs) or a therapeutic population of TILs manufactured by the method of any of claims 1-291.

[0330] A pharmaceutical composition comprising the expanded number of TILs or therapeutic population of TILs provided herein and a pharmaceutically acceptable carrier.

[0331] A population of TILs manufactured using the method disclosed herein.

[0332] A pharmaceutical composition comprising the population of TILs disclosed herein.

[0333] The pharmaceutical composition disclosed herein, further comprising an ICI.

[0334] The pharmaceutical composition disclosed herein, wherein the ICI is an inhibitor of a gene selected from the group consisting of PD-1, CTLA-4, LAG-3, HAVCR2 (TIM-3), Cish, TGF[L PKA, CBL-B, PPP2CA, PPP2CB, PTPN6, PTPN22, PDCD1, BTLA, CD 160, TIGIT, TET2, BAFF (BR3), CD96, CRTAM, LAIR1, SIGLEC7, SIGLEC9, CD244, TNFRSF10B, TNFRSF10A, CASP8, CASP10, CASP3, CASP6, CASP7, FADD, FAS, SMAD2, SMAD3, SMAD4, SMAD10, SKI, SKIL, TGTF1, IL10RA, IL10RB, HM0X2, TL6R, IL6ST, EIF2AK4, CSK, PAG1, SIT1, FOXP3, PRDM1 , BATF, GUCY1A2, GUCY1A3, GUCY1B2, GUCY1B3, TOX, SOCS1, ANKRD11, and BCOR.

[0335] In some embodiments, the present disclosures provide a method of treating a cancer patient by administering the population of TILs disclosed herein or the pharmaceutical composition disclosed herein to the cancer patient, wherein the cancer patient has received at least one prior therapy, and the cancer patient exhibits cancer progression on or after the at least one prior therapy.BRIEF DESCRIPTION OF THE DRAWINGS

[0336] Figure 1 : Exemplary Gen 2 (Process 2A) type chart providing an overview of Steps A through F.

[0337] Figures 2A-2C: Exemplary process flow chart of Gen 2 (Process 2A) type process.

[0338] Figure 3: Shows a diagram of an embodiment of a cryopreserved TIL exemplary manufacturing process (~22 days).

[0339] Figure 4: Shows a diagram of an embodiment of Gen 2, a 22 -day process for TIL manufacturing.

[0340] Figure 5: Comparison table of Steps A through F from exemplary embodiments of process 1C and Gen 2.

[0341] Figure 6: Detailed comparison of an embodiment of process 1C and an embodiment of Gen 2.

[0342] Figure 7: Exemplary Gen 3 type process for NSCLC tumors.

[0343] Figure 8A-8D: A) Shows a comparison between the 2A process (approximately 22 -day process) and an embodiment of the Gen 3 process for TIL manufacturing (approximately 14-days to 16-days process). B) Exemplary Process Gen 3 chart providing an overview of Steps A through F (approximately 14-days to 16-days process). C) Chart providing three exemplary Gen 3 processes with an overview of Steps A through F (approximately 14-days to 16-days process) for each of the three process variations. D) Exemplary Modified Gen 2-like process providing an overview of Steps A through F (approximately 22-days process).

[0344] Figure 9 : Provides an experimental flow chart for comparability between Gen 2 (Gen 2) versus Gen 3.

[0345] Figure 10: Shows a comparison between various Gen 2 (2A process) and the Gen 3.1 process embodiment.

[0346] Figure 11: Table describing various features of embodiments of the Gen 2, Gen 2.1 and Gen 3.0 process.

[0347] Figure 12: Overview of the media conditions for an embodiment of the Gen 3 process, referred to as Gen 3.1.

[0348] Figure 13: Table describing various features of embodiments of the Gen 2, Gen 2.1 and Gen 3.0 process.

[0349] Figure 14: Table comparing various features of embodiments of the Gen 2 and Gen 3.0 processes.

[0350] Figure 15: Table providing media uses in the various embodiments of the described expansion processes.

[0351] Figure 16: Schematic of an exemplary embodiment of the Gen 3 process (a 16-day process).

[0352] Figure 17: Schematic of an exemplary embodiment of a method for expanding T cells from hematopoietic malignancies using Gen 3 expansion platform.

[0353] Figure 18: Provides the structures I-A and I-B, the cylinders refer to individual polypeptide binding domains. Structures I-A and I-B comprise three linearly -linked TNFRSF binding domains derived from e.g . 4-1BBL or an antibody that binds 4-1BB, which fold to form a trivalent protein, which is then linked to a second trivalent protein through IgGl-Fc (including CH3 and CH2 domains) is then used to link two of the trivalent proteins together through disulfide bonds (small elongated ovals), stabilizing the structure and providing an agonists capable of bringing together the intracellular signaling domains of the six receptors and signaling proteins to form a signaling complex. The TNFRSF binding domains denoted as cylinders may be scFv domains comprising, e.g., a VH and a VL chain connected by a linker that may comprise hydrophilic residues and Gly and Ser sequences for flexibility, as well as Gin and Lys for solubility.

[0354] Figure 19: Schematic of an exemplary embodiment of the Gen 3 process (a 16-day process).

[0355] Figure 20: Provides a process overview for an exemplary embodiment of the Gen 3.1 process (a 16 day process).

[0356] Figure 21: Schematic of an exemplary embodiment of the Gen 3.1 Test process (a 16-17 day process).

[0357] Figure 22: Schematic of an exemplary embodiment of the Gen 3 process (a 16-day process).

[0358] Figure 23A-23B: Comparison table for exemplary Gen 2 and exemplary Gen 3 processes.

[0359] Figure 24: Schematic of an exemplary embodiment of the Gen 3 process (a 16 / 17 day process) preparation timeline.

[0360] Figure 25: Schematic of an exemplary embodiment of the Gen 3 process (a 14-16 day process).

[0361] Figure 26A-26B: Schematic of an exemplary embodiment of the Gen 3 process (a 16 day process).

[0362] Figure 27: Schematic of an exemplary embodiment of the Gen 3 process (a 16 day process).

[0363] Figure 28: Comparison of Gen 2. Gen 2.1 and an embodiment of the Gen 3 process (a 16 day process).

[0364] Figure 29: Comparison of Gen 2. Gen 2.1 and an embodiment of the Gen 3 process (a 16 day process).

[0365] Figure 30: Gen 3 embodiment components.

[0366] Figure 31 : Gen 3 embodiment flow chart comparison (Gen 3.0, Gen 3.1 control, Gen 3.1 Test).

[0367] Figure 32: Shown are the components of an exemplary embodiment of the Gen 3 process (a 16-17 day process).

[0368] Figure 33: Acceptance criteria table.

[0369] Figure 34: Diagram of Study Design related to study described in Example 19.

[0370] Figure 35: Schematic of TIL-based immunotherapy manufacturing process related to the study described in Example 19. Abbreviations: CMO = contract manufacturing organization; GMP = Good Manufacturing Practices; IL-2 = interleukin-2; OKT3 = monoclonal antibody to CD3; TIL = tumor infiltrating lymphocytes.

[0371] Figure 36: Study Flowchart (all four cohorts).

[0372] Figure 37: Patient journey and central Gen 2 GMP manufacturing.

[0373] Figure 38: Cohort 3B patient treatment schema.

[0374] Figure 39: Patient disposition.

[0375] Figure 40: Adverse events over time (FAS).

[0376] Figure 41: Best percentage change from baseline in target lesion sum of diameters (efficacy -evaluable set).

[0377] Figure 42: Time to first response, duration of response, and time on efficacy assessment for confirmed responders who achieved PR or better.

[0378] Figure 43: Percentage change from baseline in target lesion sum of diameters (FAS).

[0379] Figure 44: TIL manufacturing and patient journey.

[0380] Figure 45: Study design and endpoints.

[0381] Figure 46: Treatment schema.

[0382] Figure 47: Treatment schema for patients with post- and pre -progression tumor harvest.

[0383] Figure 48A-48D: Schematics of exemplary embodiments of the KO TIL TALEN process.

[0384] Figure 49: Experimental flow diagram of full-scale PD-1 KO TIL TALEN process.

[0385] Figure 50: Experimental flow diagram of full-scale PD-1 KO TIL TALEN process.

[0386] Figure 51A-51J: Exemplary membrane anchored immunomodulatory fusion proteins that can be included in the TILs described herein.

[0387] Figure 52A-52D: Exemplary membrane anchored immunomodulatory fusion proteins that can be included in the TILs described herein.BRIEF DESCRIPTION OF THE SEQUENCE LISTING

[0388] SEQ ID NO: 1 is the amino acid sequence of the heavy chain of muromonab.

[0389] SEQ ID NO:2 is the amino acid sequence of the light chain of muromonab.

[0390] SEQ ID NO:3 is the amino acid sequence of a recombinant human IL-2 protein.

[0391] SEQ ID NO:4 is the amino acid sequence of aldesleukin.

[0392] SEQ ID NO:5 is an IL-2 form.

[0393] SEQ ID NO:6 is the amino acid sequence of nemvaleukin alfa.

[0394] SEQ ID NO:7 is an IL-2 form.

[0395] SEQ ID NO:8 is a mucin domain polypeptide.

[0396] SEQ ID NO:9 is the amino acid sequence of a recombinant human IL-4 protein.

[0397] SEQ ID NO: 10 is the amino acid sequence of a recombinant human IL-7 protein.

[0398] SEQ ID NO: 11 is the amino acid sequence of a recombinant human IL-15 protein.

[0399] SEQ ID NO: 12 is the amino acid sequence of a recombinant human IL-21 protein.

[0400] SEQ ID NO: 13 is an IL-2 sequence.

[0401] SEQ ID NO: 14 is an IL-2 mutein sequence.

[0402] SEQ ID NO: 15 is an IL-2 mutein sequence.

[0403] SEQ ID NO: 16 is the HCDR1 IL-2 for IgG.IL2R67A.Hl.

[0404] SEQ ID NO: 17 is the HCDR2 for IgG.IL2R67A.Hl.

[0405] SEQ ID NO: 18 is the HCDR3 for IgG.IL2R67A.Hl.

[0406] SEQ ID NO: 19 is the HCDR1 IL-2 kabat for IgG.IL2R67A.Hl.

[0407] SEQ ID NO:20 is the HCDR2 kabat for IgG.IL2R67A.Hl .

[0408] SEQ ID NO:21 is the HCDR3 kabat for IgG.IL2R67A.Hl.

[0409] SEQ ID NO:22 is the HCDR1 IL-2 clothia for IgG.IL2R67A.Hl.

[0410] SEQ ID NO:23 is the HCDR2 clothia for IgG.IL2R67A.Hl .

[0411] SEQ ID NO:24 is the HCDR3 clothia for IgG.IL2R67A.Hl.

[0412] SEQ ID NO:25 is the HCDR1 IL-2 IMGT for IgG.IL2R67A.Hl.

[0413] SEQ ID NO:26 is the HCDR2 IMGT for IgG.IL2R67A.Hl.

[0414] SEQ ID NO:27 is the HCDR3 IMGT for IgG.IL2R67A.Hl.

[0415] SEQ ID NO:28 is the VH chain for IgG.IL2R67A.Hl .

[0416] SEQ ID NO:29 is the heavy chain for IgG.IL2R67A.Hl .

[0417] SEQ ID NQ:30 is the LCDR1 kabat for IgG.IL2R67A.Hl.

[0418] SEQ ID NO:31 is the LCDR2 kabat for IgG.IL2R67A.Hl.

[0419] SEQ ID NO:32 is the LCDR3 kabat for IgG.IL2R67A.Hl.

[0420] SEQ ID NO:33 is the LCDR1 chothia for IgG.IL2R67A.Hl .

[0421] SEQ ID NO:34 is the LCDR2 chothia for IgG.IL2R67A.Hl .

[0422] SEQ ID NO:35 is the LCDR3 chothia for IgG.IL2R67A.Hl .

[0423] SEQ ID NO:36 is a VL chain.

[0424] SEQ ID NO:37 is a light chain.

[0425] SEQ ID NO:38 is a light chain.

[0426] SEQ ID NO:39 is a light chain.

[0427] SEQ ID NO:40 is the amino acid sequence of human 4-1BB.

[0428] SEQ ID NO:41 is the amino acid sequence of murine 4- IBB.

[0429] SEQ ID NO:42 is the heavy chain for the 4-1BB agonist monoclonal antibody utomilumab (PF-05082566).

[0430] SEQ ID NO:43 is the light chain for the 4-1BB agonist monoclonal antibody utomilumab (PF-05082566).

[0431] SEQ ID NO:44 is the heavy chain variable region (VH) for the 4-1BB agonist monoclonal antibody utomilumab (PF-05082566).

[0432] SEQ ID NO:45 is the light chain variable region (VL) for the 4-1BB agonist monoclonal antibody utomilumab (PF-05082566).

[0433] SEQ ID NO:46 is the heavy chain CDR1 for the 4- IBB agonist monoclonal antibody utomilumab (PF-05082566).

[0434] SEQ ID NO:47 is the heavy chain CDR2 for the 4- IBB agonist monoclonal antibody utomilumab (PF-05082566).

[0435] SEQ ID NO:48 is the heavy chain CDR3 for the 4- IBB agonist monoclonal antibody utomilumab (PF-05082566).

[0436] SEQ ID NO:49 is the light chain CDR1 for the 4- IBB agonist monoclonal antibody utomilumab (PF-05082566).

[0437] SEQ ID NO:50 is the light chain CDR2 for the 4-1BB agonist monoclonal antibody utomilumab (PF-05082566).

[0438] SEQ ID NO:51 is the light chain CDR3 for the 4-1BB agonist monoclonal antibody utomilumab (PF-05082566).

[0439] SEQ ID NO:52 is the heavy chain for the 4-1BB agonist monoclonal antibody urelumab (BMS-663513).

[0440] SEQ ID NO:53 is the light chain for the 4-1BB agonist monoclonal antibody urelumab (BMS-663513).

[0441] SEQ ID NO:54 is the heavy chain variable region (VH) for the 4-1BB agonist monoclonal antibody urelumab (BMS-663513).

[0442] SEQ ID NO:55 is the light chain variable region (VL) for the 4-1BB agonist monoclonal antibody urelumab (BMS-663513).

[0443] SEQ ID NO:56 is the heavy chain CDR1 for the 4-1BB agonist monoclonal antibody urelumab (BMS-663513).

[0444] SEQ ID NO:57 is the heavy chain CDR2 for the 4-1BB agonist monoclonal antibody urelumab (BMS-663513).

[0445] SEQ ID NO:58 is the heavy chain CDR3 for the 4-1BB agonist monoclonal antibody urelumab (BMS-663513).

[0446] SEQ ID NO:59 is the light chain CDR1 for the 4-1BB agonist monoclonal antibody urelumab (BMS-663513).

[0447] SEQ ID NO:60 is the light chain CDR2 for the 4-1BB agonist monoclonal antibody urelumab (BMS-663513).

[0448] SEQ ID NO:61 is the light chain CDR3 for the 4-1BB agonist monoclonal antibody urelumab (BMS-663513).

[0449] SEQ ID NO:62 is an Fc domain for a TNFRSF agonist fusion protein.

[0450] SEQ ID NO:63 is a linker for a TNFRSF agonist fusion protein.

[0451] SEQ ID NO:64 is a linker for a TNFRSF agonist fusion protein.

[0452] SEQ ID NO:65 is a linker for a TNFRSF agonist fusion protein.

[0453] SEQ ID NO:66 is a linker for a TNFRSF agonist fusion protein.

[0454] SEQ ID NO:67 is a linker for a TNFRSF agonist fusion protein.

[0455] SEQ ID NO:68 is a linker for a TNFRSF agonist fusion protein.

[0456] SEQ ID NO:69 is a linker for a TNFRSF agonist fusion protein.

[0457] SEQ ID NO:70 is a linker for a TNFRSF agonist fusion protein.

[0458] SEQ ID NO:71 is a linker for a TNFRSF agonist fusion protein.

[0459] SEQ ID NO:72 is a linker for a TNFRSF agonist fusion protein.

[0460] SEQ ID NO:73 is an Fc domain for a TNFRSF agonist fusion protein.

[0461] SEQ ID NO:74 is a linker for a TNFRSF agonist fusion protein.

[0462] SEQ ID NO:75 is a linker for a TNFRSF agonist fusion protein.

[0463] SEQ ID NO:76 is a linker for a TNFRSF agonist fusion protein.

[0464] SEQ ID NO:77 is a 4-1BB ligand (4-1BBL) amino acid sequence.

[0465] SEQ ID NO:78 is a soluble portion of 4-1BBL polypeptide.

[0466] SEQ ID NO:79 is a heavy chain variable region (VH) for the 4-1BB agonist antibody 4B4- 1-1 version 1.

[0467] SEQ ID NO:80 is a light chain variable region (VL) for the 4-1BB agonist antibody 4B4-1-1 version 1.

[0468] SEQ ID NO:81 is a heavy chain variable region (VH) for the 4-1BB agonist antibody 4B4- 1-1 version 2.

[0469] SEQ ID NO:82 is a light chain variable region VL) for the 4-1BB agonist antibody 4B4-1-1 version 2.

[0470] SEQ ID NO:83 is a heavy chain variable region (VH) for the 4-1BB agonist antibody H39E3-2.

[0471] SEQ ID NO:84 is a light chain variable region (V ) for the 4-1BB agonist antibody H39E3- 2.

[0472] SEQ ID NO:85 is the amino acid sequence of human 0X40.

[0473] SEQ ID NO:86 is the amino acid sequence of murine 0X40.

[0474] SEQ ID NO:87 is the heavy chain for the 0X40 agonist monoclonal antibody tavolixizumab (MEDI-0562).

[0475] SEQ ID NO:88 is the light chain for the 0X40 agonist monoclonal antibody tavolixizumab (MEDI-0562).

[0476] SEQ ID NO:89 is the heavy chain variable region (VH) for the 0X40 agonist monoclonal antibody tavolixizumab (MEDI-0562).

[0477] SEQ ID NO:90 is the light chain variable region (VL) for the 0X40 agonist monoclonal antibody tavolixizumab (MEDI-0562).

[0478] SEQ ID NO:91 is the heavy chain CDR1 for the 0X40 agonist monoclonal antibody tavolixizumab (MEDI-0562).

[0479] SEQ ID NO:92 is the heavy chain CDR2 for the 0X40 agonist monoclonal antibody tavolixizumab (MEDI-0562).

[0480] SEQ ID NO:93 is the heavy chain CDR3 for the 0X40 agonist monoclonal antibody tavolixizumab (MEDI-0562).

[0481] SEQ ID NO:94 is the light chain CDR1 for the 0X40 agonist monoclonal antibody tavolixizumab (MEDI-0562).

[0482] SEQ ID NO:95 is the light chain CDR2 for the 0X40 agonist monoclonal antibody tavolixizumab (MEDI-0562).

[0483] SEQ ID NO:96 is the light chain CDR3 for the 0X40 agonist monoclonal antibody tavolixizumab (MEDI-0562).

[0484] SEQ ID NO:97 is the heavy chain for the 0X40 agonist monoclonal antibody 11D4.

[0485] SEQ ID NO:98 is the light chain for the 0X40 agonist monoclonal antibody 11D4.

[0486] SEQ ID NO:99 is the heavy chain variable region (VH) for the 0X40 agonist monoclonal antibody 11D4.

[0487] SEQ ID NO: 100 is the light chain variable region (VL) for the 0X40 agonist monoclonal antibody 11D4.

[0488] SEQ ID NO: 101 is the heavy chain CDR1 for the 0X40 agonist monoclonal antibody 11D4.

[0489] SEQ ID NO: 102 is the heavy chain CDR2 for the 0X40 agonist monoclonal antibody 11D4.

[0490] SEQ ID NO: 103 is the heavy chain CDR3 for the 0X40 agonist monoclonal antibody 11D4.

[0491] SEQ ID NO: 104 is the light chain CDR1 for the 0X40 agonist monoclonal antibody 11D4.

[0492] SEQ ID NO: 105 is the light chain CDR2 for the 0X40 agonist monoclonal antibody 11 D4.

[0493] SEQ ID NO: 106 is the light chain CDR3 for the 0X40 agonist monoclonal antibody 11 D4.

[0494] SEQ ID NO: 107 is the heavy chain for the 0X40 agonist monoclonal antibody 18D8.

[0495] SEQ ID NO: 108 is the light chain for the 0X40 agonist monoclonal antibody 18D8.

[0496] SEQ ID NO: 109 is the heavy chain variable region (VH) for the 0X40 agonist monoclonal antibody 18D8.

[0497] SEQ ID NO: 110 is the light chain variable region (VL) for the 0X40 agonist monoclonal antibody 18D8.

[0498] SEQ ID NO: 111 is the heavy chain CDR1 for the 0X40 agonist monoclonal antibody 18D8.

[0499] SEQ ID NO: 112 is the heavy chain CDR2 for the 0X40 agonist monoclonal antibody 18D8.

[0500] SEQ ID NO: 113 is the heavy chain CDR3 for the 0X40 agonist monoclonal antibody 18D8.

[0501] SEQ ID NO: 114 is the light chain CDR1 for the 0X40 agonist monoclonal antibody 18D8.

[0502] SEQ ID NO: 115 is the light chain CDR2 for the 0X40 agonist monoclonal antibody 18D8.

[0503] SEQ ID NO: 116 is the light chain CDR3 for the 0X40 agonist monoclonal antibody 18D8.

[0504] SEQ ID NO: 117 is the heavy chain variable region (VH) for the 0X40 agonist monoclonal antibody Hui 19-122.

[0505] SEQ ID NO: 118 is the light chain variable region (VL) for the 0X40 agonist monoclonal antibody Hui 19-122.

[0506] SEQ ID NO: 119 is the heavy chain CDR1 for the 0X40 agonist monoclonal antibody Hui 19-122.

[0507] SEQ ID NO: 120 is the heavy chain CDR2 for the 0X40 agonist monoclonal antibody Hui 19-122.

[0508] SEQ ID NO: 121 is the heavy chain CDR3 for the 0X40 agonist monoclonal antibody Hui 19-122.

[0509] SEQ ID NO: 122 is the light chain CDR1 for the 0X40 agonist monoclonal antibody Hui 19-122.

[0510] SEQ ID NO: 123 is the light chain CDR2 for the 0X40 agonist monoclonal antibody Hui 19-122.

[0511] SEQ ID NO: 124 is the light chain CDR3 for the 0X40 agonist monoclonal antibody Hull9-122.

[0512] SEQ ID NO: 125 is the heavy chain variable region (VH) for the 0X40 agonist monoclonal antibody Hui 06-222.

[0513] SEQ ID NO: 126 is the light chain variable region (VL) for the 0X40 agonist monoclonal antibody Hui 06-222.

[0514] SEQ ID NO: 127 is the heavy chain CDR1 for the 0X40 agonist monoclonal antibody Hul06-222.

[0515] SEQ ID NO: 128 is the heavy chain CDR2 for the 0X40 agonist monoclonal antibody Hul06-222.

[0516] SEQ ID NO: 129 is the heavy chain CDR3 for the 0X40 agonist monoclonal antibody Hul06-222.

[0517] SEQ ID NO: 130 is the light chain CDR1 for the 0X40 agonist monoclonal antibody Hul06-222.

[0518] SEQ ID NO: 131 is the light chain CDR2 for the 0X40 agonist monoclonal antibody Hul06-222.

[0519] SEQ ID NO: 132 is the light chain CDR3 for the 0X40 agonist monoclonal antibody Hul06-222.

[0520] SEQ ID NO: 133 is an 0X40 ligand (OX40L) amino acid sequence.

[0521] SEQ ID NO: 134 is a soluble portion of OX40L polypeptide.

[0522] SEQ ID NO: 135 is an alternative soluble portion of OX40L polypeptide.

[0523] SEQ ID NO: 136 is the heavy chain variable region (VH) for the 0X40 agonist monoclonal antibody 008.

[0524] SEQ ID NO: 137 is the light chain variable region (VL) for the 0X40 agonist monoclonal antibody 008.

[0525] SEQ ID NO: 138 is the heavy chain variable region (VH) for the 0X40 agonist monoclonal antibody Oil.

[0526] SEQ ID NO: 139 is the light chain variable region (VL) for the 0X40 agonist monoclonal antibody Oil.

[0527] SEQ ID NO: 140 is the heavy chain variable region (VH) for the 0X40 agonist monoclonal antibody 021.

[0528] SEQ ID NO: 141 is the light chain variable region (VL) for the 0X40 agonist monoclonal antibody 021.

[0529] SEQ ID NO: 142 is the heavy chain variable region (VH) for the 0X40 agonist monoclonal antibody 023.

[0530] SEQ ID NO: 143 is the light chain variable region (VL) for the 0X40 agonist monoclonal antibody 023.

[0531] SEQ ID NO: 144 is the heavy chain variable region (VH) for an 0X40 agonist monoclonal antibody.

[0532] SEQ ID NO: 145 is the light chain variable region (VL) for an 0X40 agonist monoclonal antibody.

[0533] SEQ ID NO: 146 is the heavy chain variable region (VH) for an 0X40 agonist monoclonal antibody.

[0534] SEQ ID NO: 147 is the light chain variable region (VL) for an 0X40 agonist monoclonal antibody.

[0535] SEQ ID NO: 148 is the heavy chain variable region (VH) for a humanized 0X40 agonist monoclonal antibody.

[0536] SEQ ID NO: 149 is the heavy chain variable region (VH) for a humanized 0X40 agonist monoclonal antibody.

[0537] SEQ ID NO: 150 is the light chain variable region (VL) for a humanized 0X40 agonist monoclonal antibody.

[0538] SEQ ID NO: 151 is the light chain variable region (VL) for a humanized 0X40 agonist monoclonal antibody.

[0539] SEQ ID NO: 152 is the heavy chain variable region (VH) for a humanized 0X40 agonist monoclonal antibody.

[0540] SEQ ID NO: 153 is the heavy chain variable region (VH) for a humanized 0X40 agonist monoclonal antibody.

[0541] SEQ ID NO: 154 is the light chain variable region (VL) for a humanized 0X40 agonist monoclonal antibody.

[0542] SEQ ID NO: 155 is the light chain variable region (VL) for a humanized 0X40 agonist monoclonal antibody.

[0543] SEQ ID NO: 156 is the heavy chain variable region (VH) for an 0X40 agonist monoclonal antibody.

[0544] SEQ ID NO: 157 is the light chain variable region (V ) for an 0X40 agonist monoclonal antibody.

[0545] SEQ ID NO: 158 is the heavy chain amino acid sequence of the PD-1 inhibitor nivolumab.

[0546] SEQ ID NO: 159 is the light chain amino acid sequence of the PD-1 inhibitor nivolumab.

[0547] SEQ ID NO: 160 is the heavy chain variable region (VH) amino acid sequence of the PD-1 inhibitor nivolumab.

[0548] SEQ ID NO: 161 is the light chain variable region (VL) amino acid sequence of the PD-1 inhibitor nivolumab.

[0549] SEQ ID NO: 162 is the heavy chain CDR1 amino acid sequence of the PD-1 inhibitor nivolumab.

[0550] SEQ ID NO: 163 is the heavy chain CDR2 amino acid sequence of the PD-1 inhibitor nivolumab.

[0551] SEQ ID NO: 164 is the heavy chain CDR3 amino acid sequence of the PD-1 inhibitor nivolumab.

[0552] SEQ ID NO: 165 is the light chain CDR1 amino acid sequence of the PD-1 inhibitor nivolumab.

[0553] SEQ ID NO: 166 is the light chain CDR2 amino acid sequence of the PD-1 inhibitor nivolumab.

[0554] SEQ ID NO: 167 is the light chain CDR3 amino acid sequence of the PD-1 inhibitor nivolumab.

[0555] SEQ ID NO: 168 is the heavy chain amino acid sequence of the PD-1 inhibitor pembrolizumab.

[0556] SEQ ID NO: 169 is the light chain amino acid sequence of the PD-1 inhibitor pembrolizumab.

[0557] SEQ ID NO: 170 is the heavy chain variable region (VH) amino acid sequence of the PD-1 inhibitor pembrolizumab.

[0558] SEQ ID NO: 171 is the light chain variable region (VL) amino acid sequence of the PD-1 inhibitor pembrolizumab.

[0559] SEQ ID NO: 172 is the heavy chain CDR1 amino acid sequence of the PD-1 inhibitor pembrolizumab.

[0560] SEQ ID NO: 173 is the heavy chain CDR2 amino acid sequence of the PD-1 inhibitor pembrolizumab.

[0561] SEQ ID NO: 174 is the heavy chain CDR3 amino acid sequence of the PD-1 inhibitor pembrolizumab.

[0562] SEQ ID NO: 175 is the light chain CDR1 amino acid sequence of the PD-1 inhibitor pembrolizumab.

[0563] SEQ ID NO: 176 is the light chain CDR2 amino acid sequence of the PD-1 inhibitor pembrolizumab.

[0564] SEQ ID NO: 177 is the light chain CDR3 amino acid sequence of the PD-1 inhibitor pembrolizumab.

[0565] SEQ ID NO: 178 is the heavy chain amino acid sequence of the PD-L1 inhibitor durvahimab.

[0566] SEQ ID NO: 179 is the light chain amino acid sequence of the PD-L1 inhibitor durvahimab.

[0567] SEQ ID NO: 180 is the heavy chain variable region (VH) amino acid sequence of the PD-L1 inhibitor durvahimab.

[0568] SEQ ID NO: 181 is the light chain variable region (VL) amino acid sequence of the PD-L1 inhibitor durvalumab.

[0569] SEQ ID NO: 182 is the heavy chain CDR1 amino acid sequence of the PD-L1 inhibitor durvalumab

[0570] SEQ ID NO: 183 is the heavy chain CDR2 amino acid sequence of the PD-L1 inhibitor durvalumab.

[0571] SEQ ID NO: 184 is tire heavy chain CDR3 amino acid sequence of the PD-L1 inhibitor durvalumab.

[0572] SEQ ID NO: 185 is the light chain CDR1 amino acid sequence of the PD-L1 inhibitor durvalumab.

[0573] SEQ ID NO: 186 is the light chain CDR2 amino acid sequence of the PD-L1 inhibitor durvalumab.

[0574] SEQ ID NO: 187 is the light chain CDR3 amino acid sequence of the PD-L1 inhibitor durvalumab.

[0575] SEQ ID NO: 188 is the heavy chain amino acid sequence of the PD-L1 inhibitor avelumab.

[0576] SEQ ID NO: 189 is the light chain amino acid sequence of the PD-L1 inhibitor avelumab.

[0577] SEQ ID NO: 190 is the heavy chain variable region (VH) amino acid sequence of the PD-L1 inhibitor avelumab.

[0578] SEQ ID NO: 191 is the light chain variable region (VL) amino acid sequence of the PD-L1 inhibitor avelumab.

[0579] SEQ ID NO: 192 is the heavy chain CDR1 amino acid sequence of the PD-L1 inhibitor avelumab.

[0580] SEQ ID NO: 193 is the heavy chain CDR2 amino acid sequence of the PD-L1 inhibitor avelumab.

[0581] SEQ ID NO: 194 is the heavy chain CDR3 amino acid sequence of the PD-L1 inhibitor avelumab.

[0582] SEQ ID NO: 195 is the light chain CDR1 amino acid sequence of the PD-L1 inhibitor avelumab.

[0583] SEQ ID NO: 196 is the light chain CDR2 amino acid sequence of the PD-L1 inhibitor avelumab.

[0584] SEQ ID NO: 197 is the light chain CDR3 amino acid sequence of the PD-L1 inhibitor avelumab.

[0585] SEQ ID NO: 198 is the heavy chain amino acid sequence of the PD-L1 inhibitor atezolizumab.

[0586] SEQ ID NO: 199 is the light chain amino acid sequence of the PD-L1 inhibitor atezolizumab.

[0587] SEQ ID N0:200 is the heavy chain variable region (VH) amino acid sequence of the PD-L1 inhibitor atezolizumab.

[0588] SEQ ID NO:201 is the light chain variable region (VL) amino acid sequence of the PD-L1 inhibitor atezolizumab.

[0589] SEQ ID NO:202 is the heavy chain CDR1 amino acid sequence of the PD-L1 inhibitor atezolizumab.

[0590] SEQ ID NO:203 is the heavy chain CDR2 amino acid sequence of the PD-L1 inhibitor atezolizumab.

[0591] SEQ ID NO:204 is the heavy chain CDR3 amino acid sequence of the PD-L1 inhibitor atezolizumab.

[0592] SEQ ID NO:205 is the light chain CDR1 amino acid sequence of the PD-L1 inhibitor atezolizumab.

[0593] SEQ ID NO:206 is the light chain CDR2 amino acid sequence of the PD-L1 inhibitor atezolizumab.

[0594] SEQ ID NO:207 is the light chain CDR3 amino acid sequence of the PD-L1 inhibitor atezolizumab.

[0595] SEQ ID NO:208 is the heavy chain amino acid sequence of the CTLA-4 inhibitor ipilimumab.

[0596] SEQ ID NO:209 is the light chain amino acid sequence of the CTLA-4 inhibitor ipilimumab.

[0597] SEQ ID NO:210 is the heavy chain variable region (VH) amino acid sequence of the CTLA- 4 inhibitor ipilimumab.

[0598] SEQ ID NO:21 1 is the light chain variable region (VL) amino acid sequence of the CTLA-4 inhibitor ipilimumab.

[0599] SEQ ID NO:212 is the heavy chain CDR1 amino acid sequence of the CTLA-4 inhibitor ipilimumab.

[0600] SEQ ID NO:213 is the heavy chain CDR2 amino acid sequence of the CTLA-4 inhibitor ipilimumab.

[0601] SEQ ID NO:214 is the heavy chain CDR3 amino acid sequence of the CTLA-4 inhibitor ipilimumab.

[0602] SEQ ID NO:215 is the light chain CDR1 amino acid sequence of the CTLA-4 inhibitor ipilimumab.

[0603] SEQ ID NO:216 is the light chain CDR2 amino acid sequence of the CTLA-4 inhibitor ipilimumab.

[0604] SEQ ID NO:217 is the light chain CDR3 amino acid sequence of the CTLA-4 inhibitor ipilimumab.

[0605] SEQ ID NO:218 is the heavy chain amino acid sequence of the CTLA-4 inhibitor tremelimumab.

[0606] SEQ ID NO:219 is the light chain amino acid sequence of the CTLA-4 inhibitor tremelimumab.

[0607] SEQ ID NO:220 is the heavy chain variable region (VH) amino acid sequence of the CTLA- 4 inhibitor tremelimumab.

[0608] SEQ ID NO:221 is the light chain variable region (VL) amino acid sequence of the CTLA-4 inhibitor tremelimumab.

[0609] SEQ ID NO:222 is the heavy chain CDR1 amino acid sequence of the CTLA-4 inhibitor tremelimumab.

[0610] SEQ ID NO:223 is the heavy chain CDR2 amino acid sequence of the CTLA-4 inhibitor tremelimumab.

[0611] SEQ ID NO:224 is the heavy chain CDR3 amino acid sequence of the CTLA-4 inhibitor tremelimumab.

[0612] SEQ ID NO:225 is the light chain CDR1 amino acid sequence of the CTLA-4 inhibitor tremelimumab.

[0613] SEQ ID NO:226 is the light chain CDR2 amino acid sequence of the CTLA-4 inhibitor tremelimumab.

[0614] SEQ ID NO:227 is the light chain CDR3 amino acid sequence of the CTLA-4 inhibitor tremelimumab.

[0615] SEQ ID NO:228 is the heavy chain amino acid sequence of the CTLA-4 inhibitor zalifrelimab.

[0616] SEQ ID NO:229 is the light chain amino acid sequence of the CTLA-4 inhibitor zalifrelimab.

[0617] SEQ ID NO:230 is the heavy chain variable region (VH) amino acid sequence of the CTLA- 4 inhibitor zalifrelimab.

[0618] SEQ ID NO:231 is the light chain variable region (VL) amino acid sequence of the CTLA-4 inhibitor zalifrelimab.

[0619] SEQ ID NO:232 is the heavy chain CDR1 amino acid sequence of the CTLA-4 inhibitor zalifrelimab.

[0620] SEQ ID NO:233 is the heavy chain CDR2 amino acid sequence of the CTLA-4 inhibitor zalifrelimab.

[0621] SEQ ID NO:234 is the heavy chain CDR3 amino acid sequence of the CTLA-4 inhibitor zalifrelimab.

[0622] SEQ ID NO:235 is the light chain CDR1 amino acid sequence of the CTLA-4 inhibitor zalifrelimab.

[0623] SEQ ID NO:236 is the light chain CDR2 amino acid sequence of the CTLA-4 inhibitor zalifrelimab.

[0624] SEQ ID NO:237 is the light chain CDR3 amino acid sequence of the CTLA-4 inhibitor zalifrelimab.

[0625] SEQ ID NO:238 is a CD8a transmembrane domain.

[0626] SEQ ID NO:239 is a B7-1 transmembrane-intracellular domain

[0627] SEQ ID NOs:240-245 are exemplary glycine-serine linkers that are useful in the immunomodulatory fusion proteins described herein.

[0628] SEQ ID NO:246 is an exemplary linker that is useful in the immunomodulatory fusion proteins described herein.

[0629] SEQ ID NO:247 is a 2A peptide C-terminus sequence.

[0630] SEQ ID NO:248 is a porcine tescliovirus-1 2A peptide.

[0631] SEQ ID NO:249 is an equine rhinitis A virus 2A peptide.

[0632] SEQ ID NO:250 is a foot-and-mouth disease virus 2A peptide.

[0633] SEQ ID NO:251 is an exemplary furin-cleavable 2A peptide.

[0634] SEQ ID NOs:252 and 253 are human IgE signal peptide sequences.

[0635] SEQ ID NO:254 is a human IL-2 signal peptide sequence.

[0636] SEQ ID NO:255 is a 6X NF AT IL-2 minimal promoter.

[0637] SEQ ID NO:256 is an NF AT responsive element.

[0638] SEQ ID NO:257 is a human IL-2 promoter sequence.

[0639] SEQ ID NO:258 is human IL-15 (N72D mutant).

[0640] SEQ ID NO:259 is human IL-15R-alpha-Su / Fc domain.

[0641] SEQ ID NO:260 is human IL-15R-alpha-Su (65aa truncated extracellular domain).

[0642] SEQ ID NO:261 is human IL-15 isoform 2.

[0643] SEQ ID NO:262 is human IL-15 isoform 1.

[0644] SEQ ID NO:263 is human IL-15 (without signal peptide).

[0645] SEQ ID NO:264 is human IL-15R-alpha (85 aa truncated extracellular domain).

[0646] SEQ ID NO:265 is human IL-15R-alpha (182aa truncated extracellular domain).

[0647] SEQ ID NO:266 is human IL-15R-alpha.

[0648] SEQ ID NO:267 is human IL-12 p35 subunit.

[0649] SEQ ID NO:268 is human IL-12 p40 subunit.

[0650] SEQ ID NO:269 is human IL-18

[0651] SEQ ID NO:270is a human IL-18 variant

[0652] SEQ ID NO:271 is human IL-21.

[0653] SEQ ID NO: 272 is human IL-2

[0654] SEQ ID NO:273 is human CD40L

[0655] SEQ ID NO:274 is agonistic anti-human CD40 VH (Sotigalimab)

[0656] SEQ ID NO:275 is agonistic anti-human CD40 VL (Sotigalimab)

[0657] SEQ ID NO:276 is agonistic anti-human CD40 scFv (Sotigalimab)

[0658] SEQ ID NO:277 is agonistic anti-human CD40 VH (Dacetuzumab)

[0659] SEQ ID NO:278 is agonistic anti-human CD40 VL (Dacetuzumab)

[0660] SEQ ID NO:279 is agonistic anti-human CD40 scFv (Dacetuzumab)

[0661] SEQ ID NO:280 is agonistic anti-human CD40 VH (Lucatutuzumab)

[0662] SEQ ID NO:281 is agonistic anti-human CD40 VL (Lucatutuzumab)

[0663] SEQ ID NO:282 is agonistic anti-human CD40 scFv (Lucatutuzumab)

[0664] SEQ ID NO:283 is agonistic anti-human CD40 VH (Selicrelumab)

[0665] SEQ ID NO:284 is agonistic anti-human CD40 VL (Selicrelumab)

[0666] SEQ ID NO:285 is agonistic anti-human CD40 scFv (Selicrelumab)

[0667] SEQ ID NO:286 is a target PD-1 sequence.

[0668] SEQ ID NO:287 is a target PD-1 sequence.

[0669] SEQ ID NO:288 is a repeat PD-1 left repeat sequence.

[0670] SEQ ID NO:289 is a repeat PD-1 right repeat sequence.

[0671] SEQ ID NO:290 is a repeat PD-1 left repeat sequence.

[0672] SEQ ID NO:291 is a repeat PD-1 right repeat sequence.

[0673] SEQ ID NO:292 is a PD-1 left TALEN nuclease sequence.

[0674] SEQ ID NO:293 is a PD-1 right TALEN nuclease sequence.

[0675] SEQ ID NO:294 is a PD-1 left TALEN nuclease sequence.

[0676] SEQ ID NO:295 is a PD-1 right TALEN nuclease sequence.

[0677] SEQ ID NO:296 is a nucleic acid sequence that encodes for the tethered IL-15 ofSEQ ID NO: 328

[0678] SEQ ID NO:297 is a nucleic acid sequence that encodes for the tethered IL-21 fusion protein of SEQ ID NO: 331.

[0679] SEQ ID NO:298 is a nucleic acid sequence that encodes for the tethered IL-15 fusion protein of SEQ ID NO:328 and tether IL-21 fusion protein of SEQ ID NO:331.

[0680] SEQ ID NO:299 is a nucleic acid sequence that encodes for the tethered IL-12 fusion protein of SEQ ID NO:303. The nucleic acid sequence includes an NF AT promoter.

[0681] SEQ ID NO:300 is a nucleic acid sequence that encodes for the tethered IL-15 fusion protein of SEQ ID NO:328. The nucleic acid sequence includes an NF AT promoter.

[0682] SEQ ID NO:301 is a nucleic acid sequence that encodes for the tethered IL-21 fusion protein of SEQ ID NO:331. The nucleic acid sequence includes an NF AT promoter.

[0683] SEQ ID NO:302 is a nucleic acid sequence that encodes for the tethered IL-15 fusion protein of SEQ ID NO:328 and tether IL-21 fusion protein of SEQ ID NO:331. The nucleic acid sequence includes an NF AT promoter.

[0684] SEQ ID NO:303 is the amino acid sequence of an exemplary tethered IL-12 (tethered IL-12-Lrl-Ar2).

[0685] SEQ ID NO:304 is a nucleic acid sequence that encodes for the tethered IL-12 of SEQ ID NO:303.

[0686] SEQ ID NO:305 is the amino acid sequence of an exemplary tethered IL-18 (tethered IL-18-Lrl-Ar2).

[0687] SEQ ID NO:306 is a nucleic acid sequence that encodes for the tethered IL-18 of SEQ ID NO:305.

[0688] SEQ ID NO:307 is the amino acid sequence of an exemplary tethered variant IL-18 (tethered DR-IL-18 (6-27 variant)-Lrl-Ar2).

[0689] SEQ ID NO:308 is a nucleic acid sequence that encodes for the tethered variant IL-18 of SEQ ID NO:307.

[0690] SEQ ID NO:309 is the amino acid sequence of an exemplary tethered IL-12 / IL-15.

[0691] SEQ ID NO: 10 is a nucleic acid sequence that encodes for the tethered IL-12 / IL-15 of SEQ ID NO:309.

[0692] SEQ ID NO:311 is the amino acid sequence of an exemplary tethered IL-18 / IL-15.

[0693] SEQ ID NO:312 is a nucleic acid sequence that encodes for the tethered IL-18 / IL-15 of SEQ ID NO:311.

[0694] SEQ ID NO:313 is the amino acid sequence of an exemplary tethered anti-CD40scFV (APX005M).

[0695] SEQ ID NO:314 is a nucleic acid sequence that encodes for the tethered anti- CD40scFV (APX005M) of SEQ ID NO:313.

[0696] SEQ ID NO:315 is the amino acid sequence of an exemplary tethered anti-CD40scFV (Dacetuzumab).

[0697] SEQ ID NO:316 is a nucleic acid sequence that encodes for the tethered anti- CD40scFV (Dacetuzumab) of SEQ ID NO:315.

[0698] SEQ ID NO:317 is the amino acid sequence of an exemplary tethered anti-CD40scFV (Lucatutuzumab) .

[0699] SEQ ID NO:318 is a nucleic acid sequence that encodes for the tethered anti- CD40scFV (Lucatutuzumab) of SEQ ID NO:317.

[0700] SEQ ID NO:319 is the amino acid sequence of an exemplary tethered anti-CD40scFV (Selicrelumab).

[0701] SEQ ID NQ:320 is a nucleic acid sequence that encodes for the tethered anti- CD40scFV (Selicrelumab) of SEQ ID NO:319.

[0702] SEQ ID NO:321 is a nucleic acid sequence that encodes for the CD40L of SEQ ID NO:273.

[0703] SEQ ID NO:322 is the amino acid sequence an exemplary tethered CD40L / IL-15.

[0704] SEQ ID NO:323 is a nucleic acid sequence that encodes for the tethered CD40L / IL-15 of SEQ ID NO:311.

[0705] SEQ ID NO:324 is the amino acid sequence of an exemplary tethered IL-2.

[0706] SEQ ID NO:325 is a nucleic acid sequence that encodes for the tethered IL-2 of SEQ ID NO:313.

[0707] SEQ ID NO:326 is the amino acid sequence of an exemplary tethered IL-12.

[0708] SEQ ID NO:327 is a nucleic acid sequence that encodes for the tethered IL-12 of SEQ ID NO:315.

[0709] SEQ ID NO:328 is the amino acid sequence of an exemplary tethered IL-15.

[0710] SEQ ID NO:329 is a nucleic acid sequence that encodes for the tethered IL-15 of SEQ ID NO:317.

[0711] SEQ ID NO:330 is a nucleic acid sequence that encodes for GFP.

[0712] SEQ ID NO:331 is the amino acid sequence of an exemplary tethered IL-21.DETAILED DESCRIPTION OF THE INVENTIONI. Introduction

[0713] Adoptive cell therapy utilizing TILs cultured ex vivo by the Rapid Expansion Protocol (REP) has produced successful adoptive cell therapy following host immunosuppression in patients with cancer such as melanoma. Current infusion acceptance parameters rely on readouts of the composition of TILs (e.g., CD28, CD8, or CD4 positivity) and on the numerical folds of expansion and viability of the REP product.

[0714] Current REP protocols give little insight into the health of the TIL that will be infused into the patient. T cells undergo a profound metabolic shift during the course of their maturation from naive to effector T cells (see Chang, et al., Nat. Immunol. 2016, 17, 364, hereby expressly incorporated in its entirety, and in particular for the discussion and markers of anaerobic and aerobic metabolism). For example, naive T cells rely on mitochondrial respiration to produce ATP, while mature, healthy effector T cells such as TIL are highly glycolytic, relying on aerobic glycolysis to provide the bioenergetics substrates they require for proliferation, migration, activation, and antitumor efficacy.

[0715] Current TIL manufacturing and treatment processes are limited by length, cost, sterility concerns, and other factors described herein such that the potential to treat patients which are refractory to anti-PDl and as such have been severely limited. There is an urgent need to provide TIL manufacturing processes and therapies based on such processes that are appropriate for use in treating patients for whom very' few or no viable treatment options remain. The present invention meets this need by providing a shortened manufacturing process for use in generating TILs which can then be employed in the treatment of non-small cell lung carcinoma (NSCLC) patients whom are refractory to anti-PD-1 treatment.II. Definitions

[0716] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of skill in the art to which this invention belongs. All patents and publications referred to herein are incorporated by reference in their entireties.

[0717] The terms “co-administration,” “co-administering,” “administered in combination with,” “administering in combination with,” “simultaneous,” and “concurrent,” as used herein, encompass administration of two or more active pharmaceutical ingredients (in a preferred embodiment of the present invention, for example, a plurality of TILs) to a subject so that both active pharmaceutical ingredients and / or their metabolites are present in the subject at the same time. Coadministration includes simultaneous administration in separate compositions, administration at different times in separate compositions, or administration in a composition in which two or more active pharmaceutical ingredients are present. Simultaneous administration in separate compositions and administration in a composition in which both agents are present are preferred.

[0718] The term “m vivo” refers to an event that takes place in a subject's body.

[0719] The term “zfi vitro” refers to an event that takes places outside of a subject's body. In vitro assays encompass cell-based assays in which cells alive or dead are employed and may also encompass a cell-free assay in which no intact cells are employed.

[0720] The term “ex vivo” refers to an event which involves treating or performing a procedure on a cell, tissue and / or organ which has been removed from a subject’s body. Aptly, the cell, tissue and / or organ may be returned to the subject’s body in a method of surgery or treatment.

[0721] The term “rapid expansion” means an increase in the number of antigen-specific TILs of at least about 3-fold (or 4-, 5-, 6-, 7-, 8-, or 9-fold) over a period of a week, more preferably at least about 10-fold (or 20-, 30-, 40-, 50-, 60-, 70-, 80-, or 90-fold) over a period of a week, or most preferably at least about 100-fold over a period of a week. A number of rapid expansion protocols are described herein.

[0722] By “tumor infiltrating lymphocytes” or “TILs” herein is meant a population of cells originally obtained as white blood cells that have left the bloodstream of a subject and migrated into a tumor. TILs include, but are not limited to, CD8+cytotoxic T cells (lymphocytes), Thl and Thl7 CD4+T cells, natural killer cells, dendritic cells and Ml macrophages. TILs include both primary and secondary TILs. “Primary TILs” are those that are obtained from patient tissue samples as outlined herein (sometimes referred to as “freshly harvested”), and “secondary TILs” are any TIL cell populations that have been expanded or proliferated as discussed herein, including, but not limited tobulk TILs and expanded TILs (“REP TILs” or “post-REP TILs”). TIL cell populations can include genetically modified TILs.

[0723] By ‘’population of cells” (including TILs) herein is meant a number of cells that share common traits. In general, populations generally range from 1 X 106to 1 X IO10in number, with different TIL populations comprising different numbers. For example, initial growth of primary TILs in the presence of IL-2 results in a population of bulk TILs of roughly 1 x 108cells. REP expansion is generally done to provide populations of 1.5 x 109to 1.5 x 1O10cells for infusion.

[0724] By “cryopreserved TILs” herein is meant that TILs, either primary, bulk, or expanded (REP TILs), are treated and stored in the range of about -150°C to -60°C. General methods for cryopreservation are also described elsewhere herein, including in the Examples. For clarity, “cryopreserved TILs” are distinguishable from frozen tissue samples which may be used as a source of primary TILs.

[0725] By “thawed cryopreserved TILs” herein is meant a population of TILs that was previously cryopreserved and then treated to return to room temperature or higher, including but not limited to cell culture temperatures or temperatures wherein TILs may be administered to a patient.

[0726] TILs can generally be defined either biochemically, using cell surface markers, or functionally, by their ability to infiltrate tumors and effect treatment. TILs can be generally categorized by expressing one or more of the following biomarkers: CD4, CD8, TCR a(3, CD27, CD28, CD56, CCR7, CD45Ra, CD95, PD-1, and CD25. Additionally and alternatively, TILs can be functionally defined by their ability to infiltrate solid tumors upon reintroduction into a patient.

[0727] The term “cryopreservation media” or “cryopreservation medium” refers to any medium that can be used for cry opreservation of cells. Such media can include media comprising 7% to 10% DMSO. Exemplary media include CryoStor CS10, Hyperthermasol, as well as combinations thereof. The term “CS10” refers to a cry opreservation medium which is obtained from Stemcell Technologies or from Biolife Solutions. The CS10 medium may be referred to by the trade name “CryoStor® CS10”. The CS10 medium is a serum-free, animal component-free medium which comprises DMSO.

[0728] The term “central memory T cell” refers to a subset of T cells that in the human are CD45R0+ and constitutively express CCR7 (CCR7hi) and CD62L (CD62*“). The surface phenotype of central memory T cells also includes TCR, CD3, CD127 (IL-7R), and IL-15R. Transcription factors for central memory T cells include BCL-6, BCL-6B, MBD2, and BMI1. Central memory T cells primarily secret IL-2 and CD40L as effector molecules after TCR triggering. Central memory T cells are predominant in the CD4 compartment in blood, and in the human are proportionally enriched in lymph nodes and tonsils.

[0729] The term “effector memory T cell” refers to a subset of human or mammalian T cells that, like central memory T cells, are CD45R0+, but have lost the constitutive expression of CCR7 (CCR710) and are heterogeneous or low for CD62L expression (CD62L10). The surface phenotype of central memory T cells also includes TCR, CD3, CD127 (IL-7R), and IL-15R. Transcription factors for central memory T cells include BLIMP 1. Effector memory T cells rapidly secret high levels of inflammatory cytokines following antigenic stimulation, including interferon-y, IL-4, and IL-5. Effector memory T cells are predominant in the CD8 compartment in blood, and in the human are proportionally enriched in the lung, liver, and gut. CD8+ effector memory T cells carry large amounts of perforin.

[0730] The term “closed system” refers to a system that is closed to the outside environment. Any closed system appropriate for cell culture methods can be employed with the methods of the present invention. Closed systems include, for example, but are not limited to closed G-containers. Once a tumor segment is added to the closed system, the system is no opened to the outside environment until the TILs are ready to be administered to the patient.

[0731] The terms “fragmenting,” “fragment,” and “fragmented,” as used herein to describe processes for disrupting a tumor, includes mechanical fragmentation methods such as crushing, slicing, dividing, and morcellating tumor tissue as well as any other method for disrupting the physical structure of tumor tissue.

[0732] The terms “peripheral blood mononuclear cells” and “PBMCs” refers to a peripheral blood cell having a round nucleus, including lymphocytes (T cells, B cells, NK cells) and monocytes. When used as an antigen presenting cell (PBMCs are a type of antigen-presenting cell), the peripheral blood mononuclear cells are preferably irradiated allogeneic peripheral blood mononuclear cells.

[0733] The terms “peripheral blood lymphocytes” and “PBLs” refer to T cells expanded from peripheral blood. In some embodiments, PBLs are separated from whole blood or apheresis product from a donor. In some embodiments, PBLs are separated from whole blood or apheresis product from a donor by positive or negative selection of a T cell phenotype, such as the T cell phenotype of CD3+ CD45+.

[0734] The term “anti-CD3 antibody” refers to an antibody or variant thereof, e.g.. a monoclonal antibody and including human, humanized, chimeric or murine antibodies which are directed against the CD3 receptor in the T cell antigen receptor of mature T cells. Anti-CD3 antibodies include OKT- 3, also known as muromonab. Anti-CD3 antibodies also include the UHCT1 clone, also known as T3 and CD3e. Other anti-CD3 antibodies include, for example, otelixizumab, teplizumab, and visilizumab.

[0735] The term “OKT-3” (also referred to herein as “OKT3”) refers to a monoclonal antibody or biosimilar or variant thereof, including human, humanized, chimeric, or murine antibodies, directed against the CD3 receptor in the T cell antigen receptor of mature T cells, and includes commercially - available forms such as OKT-3 (30 ng / mL, MACS GMP CD3 pure, Miltenyi Biotech, Inc., San Diego, CA, USA) and muromonab or variants, conservative amino acid substitutions, glycoforms, or biosimilars thereof. The amino acid sequences of the heavy and light chains of muromonab are given in Table 1 (SEQ ID NO:1 and SEQ ID NO:2). A hybridoma capable of producing OKT-3 is deposited with the American Type Culture Collection and assigned the ATCC accession number CRL 8001. A hybridoma capable of producing OKT-3 is also deposited with European Collection of Authenticated Cell Cultures (ECACC) and assigned Catalogue No. 86022706.TABLE 1. Amino acid sequences of muromonab (exemplary OKT-3 antibody).

[0736] The term “IL-2” (also referred to herein as “IL2”) refers to the T cell growth factor known as intcrlcukin-2, and includes all forms of IL-2 including human and mammalian forms, conservative amino acid substitutions, glycoforms, biosimilars, and variants thereof. IL-2 is described, e.g., in Nelson, J. Immunol. 2004, 172, 3983-88 and Malek, Annu. Rev. Immunol. 2008, 26, 453-79, the disclosures of which are incorporated by reference herein. The amino acid sequence of recombinant human IL-2 suitable for use in the invention is given in Table 2 (SEQ ID NO:3). For example, the term IL-2 encompasses human, recombinant forms of IL-2 such as aldesleukin (PROLEUKIN, available commercially from multiple suppliers in 22 million IU per single use vials), as well as the form of recombinant IL-2 commercially supplied by CellGenix, Inc., Portsmouth, NH, USA (CELLGRO GMP) or ProSpec-Tany TechnoGene Ltd., East Brunswick, NJ, USA (Cat. No. CYT- 209-b) and other commercial equivalents from other vendors. Aldesleukin (des-alanyl- 1, serine- 125 human IL-2) is a nonglycosylated human recombinant form of IL-2 with a molecular weight of approximately 15 kDa. The amino acid sequence of aldesleukin suitable for use in the invention is given in Table 2 (SEQ ID NO:4). The term IL-2 also encompasses pegylated forms of IL-2, as described herein, including the pegylated IL2 prodrug bempegaldesleukin (NKTR-214, pegylated human recombinant IL -2 as in SEQ ID NO:4 in which an average of 6 lysine residues are N6substituted with [(2, 7-bis{ [methylpoly (oxyethylene)]carbamoyl}-9H-fluoren-9-yl)methoxy]carbonyl), which is available from Nektar Therapeutics, South San Francisco, CA, USA, or which may be prepared by methods known in the art, such as the methods described in Example 19 of International Patent Application Publication No. WO 2018 / 132496 Al or the method described in Example 1 of U.S. Patent Application Publication No. US 2019 / 0275133 Al, the disclosures of which are incorporated by reference herein. Bempegaldesleukin (NKTR-214) and other pegylated IL -2 molecules suitable for use in the invention is described in U.S. Patent Application Publication No. US 2014 / 0328791 Al and International Patent Application Publication No. WO 2012 / 065086 Al, the disclosures of which are incorporated by reference herein. Alternative forms of conjugated IL-2 suitable for use in the invention are described in U.S. Patent Nos. 4,766,106, 5,206,344, 5,089,261 and 4,902,502, the disclosures of which are incorporated by reference herein. Formulations of IL-2 suitable for use in the invention are described in U.S. Patent No. 6,706,289, the disclosure of which is incorporated by reference herein.

[0737] In some embodiments, an IL-2 form suitable for use in the present invention is THOR-707, available from Synthorx, Inc. The preparation and properties of THOR-707 and additional alternative forms of IL-2 suitable for use in the invention are described in U.S. Patent Application Publication Nos. US 2020 / 0181220 Al and US 2020 / 0330601 Al, the disclosures of which are incorporated by reference herein. In some embodiments, and IL-2 form suitable for use in the invention is an interleukin 2 (IL-2) conjugate comprising: an isolated and purified IL-2 polypeptide; and a conjugating moiety that binds to the isolated and purified IL-2 polypeptide at an amino acid position selected from K35, T37, R38, T41, F42, K43, F44, Y45, E61, E62, E68, K64, P65, V69, L72, and Y107, wherein the numbering of the amino acid residues corresponds to SEQ ID NO:5 In some embodiments, the amino acid position is selected from T37, R38, T41 , F42, F44, Y45, E61, E62, E68, K64, P65, V69, L72, and Y107. In some embodiments, the amino acid position is selected from T37, R38, T41, F42, F44, Y45, E61, E62, E68, P65, V69, L72, and Y107. In some embodiments, the amino acid position is selected from T37, T41, F42, F44, Y45, P65, V69, L72, and Y107. In some embodiments, the amino acid position is selected from R38 and K64. In some embodiments, the amino acid position is selected from E61, E62, and E68. In some embodiments, the amino acid position is at E62. In some embodiments, the amino acid residue selected from K35, T37, R38, T41, F42, K43, F44, Y45, E61, E62, E68, K64, P65, V69, L72, and Y107 is further mutated to lysine, cysteine, or histidine. In some embodiments, the amino acid residue is mutated to cysteine. In some embodiments, the amino acid residue is mutated to lysine. In some embodiments, the amino acid residue selected from K35, T37, R38, T41, F42, K43, F44, Y45, E61, E62, E68, K64, P65, V69, L72, and Y 107 is further mutated to an unnatural amino acid. In some embodiments, the unnatural amino acid comprises N6-azidoethoxy-L-lysine (AzK), N6-propargylethoxy-L-lysine (PraK), BCN-L- lysine, norbornene lysine, TCO-lysine, methyltetrazine lysine, allyloxycarbonyllysine, 2-amino-8-oxononanoic acid, 2-amino-8-oxooctanoic acid, p-acetyl-L-phenylalanine, p-azidomethyl-L- phenylalanine (pAMF), p-iodo-L-phenylalanine, m-acetylphenylalanine, 2-amino-8-oxononanoic acid, p-propargyloxyphenylalanine, p-propargyl-phenylalanine, 3-methyl-phenylalanine, L-Dopa, fluorinated phenylalanine, isopropyl-L-phenylalanine, p-azido-L-phenylalanine, p-acyl-L- phen lalanine, p-benzoyl-L-phenylalanine, p -bromophen lalanine, p-amino-L-phenylalanine, isopropyl-L-phenylalanine, O-allyltyrosine, O-methyl-L-tyrosine, O-4-allyl-L-tyrosine, 4-propyLL- tyrosine, phosphonotyrosine, tri-O-acetyl-GlcNAcp-serine, L-phosphoserine, phosphonoserine, L-3- (2-naphthyl)alanine, 2-amino-3-((2-((3-(benzyloxy)-3-oxopropyl)amino)ethyl)selanyl)propanoic acid, 2-amino-3-(phenylselanyl)propanoic, or selenocysteine. In some embodiments, the IL-2 conjugate has a decreased affinity to IL-2 receptor a (IL-2Ra) subunit relative to a wild-type IL-2 polypeptide. In some embodiments, the decreased affinity is about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 99%, or greater than 99% decrease in binding affinity to lL-2Ra relative to a wild-type 1L- 2 polypeptide. In some embodiments, the decreased affinity is about 1-fold, 2-fold, 3-fold, 4-fold, 5- fold, 6-fold, 7-fold, 8-fold, 9-fold, 10-fold, 30-fold, 50-fold, 100-fold, 200-fold, 300-fold, 500-fold, 1000-fold, or more relative to a wild-type IL-2 polypeptide. In some embodiments, the conjugating moiety impairs or blocks the binding of IL-2 with IL-2Ra. In some embodiments, the conjugating moiety comprises a water-soluble polymer. In some embodiments, the additional conjugating moiety comprises a water-soluble polymer. In some embodiments, each of the water-soluble polymers independently comprises polyethylene glycol (PEG), polypropylene glycol) (PPG), copolymers of ethylene glycol and propylene glycol, poly(oxyethylated polyol), poly(olefinic alcohol), polyvinylpyrrolidone), poly(hydroxyalkylmethacrylamide), poly(hydroxyalkylmethacrylate), poly(saccharides), poly(a-hydroxy acid), poly(vinyl alcohol), polyphosphazene, polyoxazolines (POZ), poly(N-acryloylmorpholine), or a combination thereof. In some embodiments, each of the water-soluble polymers independently comprises PEG. In some embodiments, the PEG is a linear PEG or a branched PEG. In some embodiments, each of the water-soluble polymers independently comprises a polysaccharide. In some embodiments, the polysaccharide comprises dextran, polysialic acid (PSA), hyaluronic acid (HA), amylose, heparin, heparan sulfate (HS), dextrin, or hydroxyethylstarch (HES). In some embodiments, each of the water-soluble polymers independently comprises a glycan. In some embodiments, each of the water-soluble polymers independently comprises polyamine. In some embodiments, the conjugating moiety comprises a protein. In some embodiments, the additional conjugating moiety comprises a protein. In some embodiments, each of the proteins independently comprises an albumin, a transferrin, or a transthyretin. In some embodiments, each of the proteins independently comprises an Fc portion. In some embodiments, each of the proteins independently comprises an Fc portion of IgG. In some embodiments, the conjugating moiety comprises a polypeptide. In some embodiments, the additional conjugating moiety comprises a polypeptide. In some embodiments, each of the polypeptides independently comprises a XTENpeptide, a glycine-rich homoamino acid polymer (HAP), a PAS polypeptide, an elastin-like polypeptide (ELP), a CTP peptide, or a gelatin-like protein (GLK) polymer. In some embodiments, the isolated and purified IL-2 polypeptide is modified by glutamylation. In some embodiments, the conjugating moiety is directly bound to the isolated and purified IL-2 polypeptide. In some embodiments, the conjugating moiety is indirectly bound to the isolated and purified IL-2 polypeptide through a linker. In some embodiments, the linker comprises a homobifunctional linker. In some embodiments, the homobifunctional linker comprises Lomant's reagent dithiobis (succinimidylpropionate) DSP, 3'3'-dithiobis(sulfosuccinimidyl proprionate) (DTSSP), disuccinimidyl suberate (DSS), bis(sulfosuccinimidyl)suberate (BS), disuccinimidyl tartrate (DST), disulfosuccinimidyl tartrate (sulfo DST), ethylene glycobis(succinimidylsuccinate) (EGS), disuccinimidyl glutarate (DSG), N,N'-disuccinimidyl carbonate (DSC), dimethyl adipimidate (DMA), dimethyl pimelimidate (DMP), dimethyl suberimidate (DMS), dimethyl-3,3'-dithiobispropionimidate (DTBP), l,4-di-(3'-(2'-pyridyldithio)propionamido)butane (DPDPB), bismaleimidohexane (BMH), aryl halide-containing compound (DFDNB), such as e g. l,5-difhioro-2,4-dinitrobenzene or 1,3- difhioro-4,6-dinitrobenzene, 4,4'-difluoro-3,3'-dinitrophenylsulfone (DFDNPS), bis-[f>-(4- azidosalicylamido)ethyl]disulfide (BASED), formaldehyde, glutaraldehyde, 1,4-butanediol diglycidyl ether, adipic acid dihydrazide, carbohydrazide, o-toluidine, 3,3'-dimethylbenzidine, benzidine, a,a'-p- diaminodiphenyl, diiodo-p-xylene sulfonic acid, N,N'-ethylene-bis(iodoacetamide), or N,N'- hexamethylene-bis(iodoacetamide). In some embodiments, the linker comprises a heterobifunctional linker. In some embodiments, the heterobifunctional linker comprises N-succinimidyl 3-(2- pyridyldithio)propionate (sPDP), long-chain N-succinimidyl 3-(2-pyridyldithio)propionate (LC- sPDP), water-soluble -long-chain N-succinimidyl 3-(2-pyridyldithio) propionate (sulfo-LC-sPDP), succinimidyloxycarbonyl-a-methyl-a-(2-pyridyldithio)toluene (sMPT), sulfosuccinimidyl-6-[a- methyl-a-(2-pyridyldithio)toluamido]hexanoate (sulfo-LC-sMPT), succinimidyl-4-(N- malcimidomcthyl)cyclohcxanc-l -carboxylate (sMCC), sulfosuccinimidyl-4-(N- maleimidomethyl)cyclohexane-l -carboxylate (sulfo-sMCC), m-maleimidobenzoyl-N- hydroxy succinimide ester (MBs), m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester (sulfo- MBs), N-succinimidyl(4-iodoacteyl)aminobenzoate (sIAB), sulfosuccinimidyl(4- iodoacteyl)aminobenzoate (sulfo-sIAB), succinimidyl-4-(p-maleimidophenyl)butyrate (sMPB), sulfosuccinimidyl-4-(p-maleimidophenyl)butyrate (sulfo-sMPB), N-(y- maleimidobutyryloxyjsuccinimide ester (GMBs), N-(y-maleimidobutyryloxy) sulfosuccinimide ester (sulfo-GMBs), succinimidyl 6-((iodoacetyl)amino)hexanoate (sIAX), succinimidyl 6-[6- (((iodoacetyl)amino)hexanoyl)amino]hexanoate (slAXX), succinimidyl 4- (((iodoacetyl)amino)methyl)cyclohexane-l-carboxylate (sIAC), succinimidyl 6-(((((4- iodoacetyl)amino)methyl)cyclohexane-l-carbonyl)ammo) hexanoate (sIACX), p-nitrophenyl iodoacetate (NPIA), carbonyl-reactive and sulfhydryl-reactive cross-linkers such as 4-(4-N-maleimidophenyljbutyric acid hydrazide (MPBH), 4-(N-maleimidomethyl)cyclohexane-l-carboxyl- hydrazide-8 (M2C2H), 3-(2-pyridyldithio)propionyl hydrazide (PDPH), N-hydroxysuccinimidyl-4- azidosalicylic acid (NHs-AsA), N-hydroxysulfosuccinimidyl-4-azidosalicylic acid (sulfo-NHs-AsA), sulfosuccinimidyl-(4-azidosalicylamido)hexanoate (sulfo-NHs-LC-AsA), sulfosuccinimidyl-2-(p- azidosalicylamido)ethyl-l,3'-dilhiopropionale (sAsD), N-hydroxysuccinimidyl-4-azidobenzoale (HsAB), N-hydroxysulfosuccinimidyl-4-azidobenzoate (sulfo-HsAB), N-succinimidyl-6-(4'-azido-2'- nitrophenyl amino)hexanoate (sANPAH), sulfosuccinimidyl-6-(4'-azido-2'- nitrophenylamino)hexanoate (sulfo-sANPAH), N-5-azido-2 -nitrobenzoyloxysuccinimide (ANB- NOs), sulfosuccinimidyl-2-(m-azido-o-nitrobenzamido)-ethy 1-1, 3 '-dithiopropionate (sAND), N- succinimidyl-4(4-azidopheny 1)1, 3 '-dithiopropionate (sADP), N-sulfosuccinimidyl(4-azidophenyl)- 1,3 '-dithiopropionate (sulfo-sADP), sulfosuccinimidyl 4-(p-azidophenyl)butyrate (sulfo-sAPB), sulfosuccinimidy 12-(7-azido-4-methy lcoumarin-3-acetamide)ethy 1- 1,3 '-dithiopropionate (s AED), sulfosuccinimidyl 7-azido-4-methylcoumain-3-acetate (sulfo-sAMCA), p-nitrophenyl diazopyruvate (pNPDP), p-nitropheny 1-2 -diazo-3, 3, 3 -trifluoropropionate (PNP-DTP), l-(p-azidosalicylamido)-4- (iodoacetamido)butane (AsIB), N-[4-(p-azidosalicylamido)butyl]-3'-(2'-pyridyldithio) propionamide (APDP), benzophenone-4-iodoacetamide, p-azidobenzoyl hydrazide (ABH), 4-(p- azidosalicylamido)butylamine (AsBA), or p-azidophenyl glyoxal (APG). In some embodiments, the linker comprises a cleavable linker, optionally comprising a dipeptide linker. In some embodiments, the dipeptide linker comprises Val-Cit, Phe-Lys, Val-Ala, or Val-Lys. In some embodiments, the linker comprises a non-cleavable linker. In some embodiments, the linker comprises a maleimide group, optionally comprising maleimidocaproyl (me), succinimidyl-4-(N- maleimidomethyl)cyclohexane-l -carboxylate (sMCC), or sulfosuccinimidyl-4-(N- maleimidomethyl)cyclohexane-l -carboxylate (sulfo-sMCC). In some embodiments, the linker further comprises a spacer. In some embodiments, the spacer comprises p-aminobenzyl alcohol (PAB), p- aminobenzyoxy carbonyl (PABC), a derivative, or an analog thereof. In some embodiments, the conjugating moiety is capable of extending the serum half-life of the IL-2 conjugate. In some embodiments, the additional conjugating moiety is capable of extending the serum half-life of the IL- 2 conjugate. In some embodiments, the IL-2 form suitable for use in the invention is a fragment of any of the IL-2 forms described herein. In some embodiments, the IL-2 form suitable for use in the invention is pegylated as disclosed in U.S. Patent Application Publication No. US 2020 / 0181220 Al and U.S. Patent Application Publication No. US 2020 / 0330601 AL In some embodiments, the IL-2 form suitable for use in the invention is an IL-2 conjugate comprising: an IL-2 polypeptide comprising an N6-azidoethoxy-L-lysine (AzK) covalently attached to a conjugating moiety comprising a polyethylene glycol (PEG), wherein: the IL-2 polypeptide comprises an amino acid sequence having at least 80% sequence identity to SEQ ID NO: 5; and the AzK substitutes for an amino acid at position K35, F42, F44, K43, E62, P65, R38, T41, E68, Y45, V69, or L72 in referenceto the amino acid positions within SEQ ID NO:5. In some embodiments, the IL-2 polypeptide comprises an N-terminal deletion of one residue relative to SEQ ID NO:5. In some embodiments, the IL-2 form suitable for use in the invention lacks IL-2R alpha chain engagement but retains normal binding to the intermediate affinity IL-2R beta-gamma signaling complex. In some embodiments, the IL-2 form suitable for use in the invention is an IL -2 conjugate comprising: an IL-2 polypeptide comprising an N6-azidoethoxy-L-lysine (AzK) covalently attached to a conjugating moiety comprising a polyethylene glycol (PEG), wherein: the IL-2 polypeptide comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 5; and the AzK substitutes for an amino acid at position K35, F42, F44, K43, E62, P65, R38, T41, E68, Y45, V69, or L72 in reference to the amino acid positions within SEQ ID NO:5. In some embodiments, the IL-2 form suitable for use in the invention is an IL-2 conjugate comprising: an IL-2 polypeptide comprising an N6- azidoethoxy-L-lysine (AzK) covalently attached to a conjugating moiety comprising a polyethylene glycol (PEG), wherein: the IL-2 polypeptide comprises an amino acid sequence having at least 95% sequence identity to SEQ ID NO:5; and the AzK substitutes for an amino acid at position K35, F42, F44, K43, E62, P65, R38, T41, E68, Y45, V69, or L72 in reference to the amino acid positions within SEQ ID NO:5. In some embodiments, the IL-2 form suitable for use in the invention is an IL-2 conjugate comprising: an IL-2 polypeptide comprising an N6-azidoethoxy-L-lysine (AzK) covalently attached to a conjugating moiety comprising a polyethylene glycol (PEG), wherein: the IL-2 polypeptide comprises an amino acid sequence having at least 98% sequence identity to SEQ ID NO:5; and the AzK substitutes for an amino acid at position K35, F42, F44, K43, E62, P65, R38, T41, E68, Y45, V69, or L72 in reference to the amino acid positions within SEQ ID NO:5.

[0738] In some embodiments, an IL-2 form suitable for use in the invention is nemvaleukin alfa, also known as ALKS-4230 (SEQ ID NO:6), which is available from Alkermes, Inc.Nemvaleukin alfa is also known as human interleukin 2 fragment (1-59), variant (Cys125>Ser51), fused via peptidyl linker (60GG61) to human interleukin 2 fragment (62-132), fused via peptidyl linker (133GSGGGS138) to human interleukin 2 receptor ot-chain fragment (139-303), produced in Chinese hamster ovary (CHO) cells, glycosylated; human interleukin 2 (IL-2) (75-133)-peptide [Cys125(51)>Ser]-mutant (1-59), fused via a Gi peptide linker (60-61) to human interleukin 2 (IL-2) (4-74)-peptide (62-132) and via a GSG3S peptide linker (133-138) to human interleukin 2 receptor a- chain (IL2R subunit alpha, IL2Ra, IL2RA) (l-165)-peptide (139-303), produced in Chinese hamster ovary (CHO) cells, glycoform alfa. The amino acid sequence of nemvaleukin alfa is given in SEQ ID NO:6. In some embodiments, nemvaleukin alfa exhibits the following post-translational modifications: disulfide bridges at positions: 31-116, 141-285, 184-242, 269-301, 166-197 or 166- 199, 168-199 or 168-197 (using the numbering in SEQ ID NO:6), and glycosylation sites at positions: N187, N206, T212 using the numbering in SEQ ID NO:6. The preparation and properties of nemvaleukin alfa, as well as additional alternative forms of IL-2 suitable for use in the invention, isdescribed in U.S. Patent Application Publication No. US 2021 / 0038684 Al and U.S. Patent No. 10,183,979, the disclosures of which are incorporated by reference herein. In some embodiments, an IL-2 form suitable for use in the invention is a protein having at least 80%, at least 90%, at least 95%, or at least 90% sequence identity to SEQ ID NO:6. In some embodiments, an IL-2 form suitable for use in the invention has the amino acid sequence given in SEQ ID NO:6 or conservative amino acid substitutions thereof. In some embodiments, an IL-2 form suitable for use in the invention is a fusion protein comprising amino acids 24-452 of SEQ ID NO:7, or variants, fragments, or derivatives thereof. In some embodiments, an IL-2 form suitable for use in the invention is a fusion protein comprising an amino acid sequence having at least 80%, at least 90%, at least 95%, or at least 90% sequence identity to amino acids 24-452 of SEQ ID NO:7, or variants, fragments, or derivatives thereof. Other IL-2 forms suitable for use in the present invention are described in U.S. Patent No. 10,183,979, the disclosures of which are incorporated by reference herein. Optionally, in some embodiments, an IL-2 form suitable for use in the invention is a fusion protein comprising a first fusion partner that is linked to a second fusion partner by a mucin domain polypeptide linker, wherein the first fusion partner is IL-IRa or a protein having at least 98% amino acid sequence identity to IL- IRct and having the receptor antagonist activity of IL-Ra, and wherein the second fusion partner comprises all or a portion of an immunoglobulin comprising an Fc region, wherein the mucin domain polypeptide linker comprises SEQ ID NO: 8 or an amino acid sequence having at least 90% sequence identity to SEQ ID NO:8 and wherein the half-life of the fusion protein is improved as compared to a fusion of the first fusion partner to the second fusion partner in the absence of the mucin domain polypeptide linker.TABLE 2. Amino acid sequences of interleukins.

[0739] In some embodiments, an IL-2 form suitable for use in the invention includes a antibody cytokine engrafted protein comprises a heavy chain variable region (VH), comprising complementarity' determining regions HCDR1, HCDR2, HCDR3; a light chain variable region (VL), comprising LCDR1, LCDR2, LCDR3; and an IL-2 molecule or a fragment thereof engrafted into a CDR of the VH or the VL, wherein the antibody cytokine engrafted protein preferentially expands T effector cells over regulatory T cells. Insome embodiments, the antibody cytokine engrafted protein comprises a heavy chain variable region (VH), comprising complementarity determining regions HCDR1, HCDR2, HCDR3; a light chain variable region (VL), comprising LCDR1, LCDR2, LCDR3; and an IL-2 molecule or a fragment thereof engrafted into a CDR of the VH or the VL, wherein the IL- 2 molecule is a mutein, and wherein the antibody cytokine engrafted protein preferentially expands T effector cells over regulatory T cells. In some embodiments, the IL-2 regimen comprises administration of an antibody described in U.S. Patent Application Publication No. US 2020 / 0270334 Al, the disclosures of which are incorporated by reference herein. In some embodiments, the antibody cytokine engrafted protein comprises a heavy chain variable region (VH), comprising complementarity' determining regions HCDR1, HCDR2, HCDR3; a light chain variable region (VL), comprising LCDR1, LCDR2, LCDR3; and an IL-2 molecule or a fragment thereof engrafted into a CDR of the VH or the VL, wherein the IL-2 molecule is a mutein, wherein the antibody cytokine engrafted protein preferentially expands T effector cells over regulatory T cells, and wherein the antibody further comprises an IgG class heavy' chain and an IgG class light chain selected from the group consisting of: a IgG class light chain comprising SEQ ID NO:39 and a IgG class heavy chain comprising SEQ ID NO:38; a IgG class light chain comprising SEQ ID NO:37 and a IgG class heavy chain comprising SEQ ID NO:29; a IgG class light chain comprising SEQ ID NO:39 and a IgG class heavy chain comprising SEQ ID NO:29; and a IgG class light chain comprising SEQ ID NO:37 and a IgG class heavy chain comprising SEQ ID NO:38.

[0740] In some embodiments, an IL-2 molecule or a fragment thereof is engrafted into HCDR1 of the VH, wherein the IL-2 molecule is a mutein. In some embodiments, an IL-2 molecule or a fragment thereof is engrafted into HCDR2 of the VH, wherein the IL-2 molecule is a mutein. In some embodiments, an IL-2 molecule or a fragment thereof is engrafted into HCDR3 of the VH, wherein the IL-2 molecule is a mutein. In some embodiments, an IL-2 molecule or a fragment thereof is engrafted into LCDR1 of the VL, wherein the IL-2 molecule is a mutein. In some embodiments, an IL-2 molecule or a fragment thereof is engrafted into LCDR2 of the VL, wherein the IL-2 molecule is a mutein. In some embodiments, an IL-2 molecule or a fragment thereof is engrafted into LCDR3 of the VL, wherein the IL-2 molecule is a mutein.

[0741] The insertion of the IL-2 molecule can be at or near the N-terminal region of the CDR, in the middle region of the CDR or at or near the C-terminal region of the CDR. In some embodiments, the antibody cytokine engrafted protein comprises an IL-2 molecule incorporated into a CDR, wherein the IL2 sequence does not frameshift the CDR sequence. In some embodiments, the antibody cytokine engrafted protein comprises an IL-2 molecule incorporated into a CDR, wherein the IL-2 sequence replaces all or part of a CDR sequence. The replacement by the IL-2 molecule can be the N-terminal region of tire CDR, in tire middle region of the CDR or at or near the C-terminal region the CDR. A replacement by the IL-2 molecule can be as few as one or two amino acids of a CDR sequence, or the entire CDR sequences.

[0742] In some embodiments, an IL-2 molecule is engrafted directly into a CDR without a peptide linker, with no additional amino acids between the CDR sequence and the IL-2 sequence. In some embodiments, an IL-2 molecule is engrafted indirectly into a CDR with a peptide linker, with one or more additional amino acids between tire CDR sequence and the IL-2 sequence.

[0743] In some embodiments, the IL-2 molecule described herein is an IL-2 mutein. In some instances, the IL-2 mutein comprising an R67A substitution. In some embodiments, the IL-2 mutein comprises the amino acid sequence SEQ ID NO: 14 or SEQ ID NO: 15. In some embodiments, the IL- 2 mutein comprises an amino acid sequence in Table 1 in U.S. Patent Application Publication No. US 2020 / 0270334 Al, the disclosure of which is incorporated by reference herein.

[0744] In some embodiments, the antibody cytokine engrafted protein comprises an HCDR1 selected from die group consisting of SEQ ID NO: 16, SEQ ID NO: 19, SEQ ID NO:22 and SEQ ID NO:25. In some embodiments, the antibody cytokine engrafted protein comprises an HCDR1 selected from the group consisting of SEQ ID NO:7, SEQ ID NO: 10, SEQ ID NO: 13 and SEQ ID NO: 16. In some embodiments, the antibody cytokine engrafted protein comprises an HCDR1 selected from the group consisting of HCDR2 selected from the group consisting of SEQ ID NO: 17, SEQ ID NO:20, SEQ ID NO:23, and SEQ ID NO:26. In some embodiments, the antibody cytokine engrafted proteincomprises an HCDR3 selected from the group consisting of SEQ ID NO: 18, SEQ ID NO:21, SEQ ID NO:24, and SEQ ID NO:27. In some embodiments, the antibody cytokine engrafted protein comprises a VH region comprising the amino acid sequence of SEQ ID NO:28. In some embodiments, the antibody cytokine engrafted protein comprises a heavy chain comprising the amino acid sequence of SEQ ID NO:29. In some embodiments, the antibody cytokine engrafted protein comprises a VL region comprising the amino acid sequence of SEQ ID NO:36. In some embodiments, the antibody cytokine engrafted protein comprises a light chain comprising the amino acid sequence of SEQ ID NO:37. In some embodiments, the antibody cytokine engrafted protein comprises a VH region comprising the amino acid sequence of SEQ ID NO:28 and a VL region comprising the amino acid sequence of SEQ ID NO:36. In some embodiments, the antibody cytokine engrafted protein comprises a heavy chain region comprising the amino acid sequence of SEQ ID NO:29 and a light chain region comprising the amino acid sequence of SEQ ID NO:37. In some embodiments, the antibody cytokine engrafted protein comprises a heavy chain region comprising the amino acid sequence of SEQ ID NO: 29 and a light chain region comprising the amino acid sequence of SEQ ID NO:39. In some embodiments, the antibody cytokine engrafted protein comprises a heavy chain region comprising the amino acid sequence of SEQ ID NO:38 and a light chain region comprising the amino acid sequence of SEQ ID NO:37. In some embodiments, the antibody cytokine engrafted protein comprises a heavy chain region comprising the amino acid sequence of SEQ ID NO:38 and a light chain region comprising the amino acid sequence of SEQ ID NO:39. In some embodiments, the antibody cytokine engrafted protein comprises IgG.IL2F71A.Hl or IgG.IL2R67A.Hl of U.S. Patent Application Publication No. 2020 / 0270334 Al, or variants, derivatives, or fragments thereof, or conservative amino acid substitutions thereof, or proteins with at least 80%, at least 90%, at least 95%, or at least 98% sequence identity thereto. In some embodiments, the antibody components of the antibody cytokine engrafted protein described herein comprise immunoglobulin sequences, framework sequences, or CDR sequences of palivizumab. In some embodiments, the antibody cytokine engrafted protein described herein has a longer serum half-life that a wild-type IL-2 molecule such as, but not limited to, aldesleukin or a comparable molecule. In some embodiments, the antibody cytokine engrafted protein described herein has a sequence as set forth in Table 3.TABLE 3: Sequences of exemplary palivizumab antibody-IL-2 engrafted proteins

[0745] The term “IL-4” (also referred to herein as “IL4”) refers to the cytokine known as interleukin 4, which is produced by Th2 T cells and by eosinophils, basophils, and mast cells. IL-4 regulates the differentiation of naive helper T cells (ThO cells) to Th2 T cells. Steinke and Borish, Respir. Res. 2001, 2, 66-70. Upon activation by IL-4, Th2 T cells subsequently produce additional IL- 4 in a positive feedback loop. IL-4 also stimulates B cell proliferation and class II MHC expression, and induces class switching to IgE and IgGi expression from B cells. Recombinant human IL-4 suitable for use in the invention is commercially available from multiple suppliers, including ProSpec- Tany TcchnoGcnc Ltd., East Brunswick, NJ, USA (Cat. No. CYT-211) and ThermoFisher Scientific, Inc., Waltham, MA, USA (human IL-15 recombinant protein, Cat. No. Gibco CTP0043). The amino acid sequence of recombinant human IL-4 suitable for use in the invention is given in Table 2 (SEQ ID NO:9).

[0746] The term “IL-7” (also referred to herein as “IL7”) refers to a glycosylated tissue-derived cytokine known as interleukin 7, which may be obtained from stromal and epithelial cells, as well as from dendritic cells. Fry and Mackall, Blood 2002, 99, 3892-904. IL-7 can stimulate the development of T cells. IL-7 binds to the IL-7 receptor, a heterodimer consisting of IL-7 receptor alpha and common gamma chain receptor, which in a series of signals important for T cell development within the thymus and survival within the periphery. Recombinant human IL-7 suitable for use in the invention is commercially available from multiple suppliers, including ProSpec-Tany TechnoGene Ltd., East Brunswick, NJ, USA (Cat. No. CYT-254) and ThermoFisher Scientific, Inc., Waltham, MA, USA (human IL-15 recombinant protein, Cat. No. Gibco PHC0071). The amino acid sequence of recombinant human IL-7 suitable for use in the invention is given in Table 2 (SEQ ID NOTO).

[0747] The term “IL-15” (also referred to herein as “IL15”) refers to the T cell growth factor known as interleukin- 15, and includes all forms of IL-2 including human and mammalian forms, conservative amino acid substitutions, glycoforms, biosimilars, and variants thereof. IL-15 is described, e.g., in Fehniger and Caligiuri, Blood 2001, 97, 14-32, the disclosure of which is incorporated by reference herein. IL-15 shares (3 and y signaling receptor subunits with IL-2. Recombinant human IL- 15 is a single, non-glycosylated polypeptide chain containing 114 amino acids (and an N-terminal methionine) with a molecular mass of 12.8 kDa. Recombinant human IL-15 is commercially available from multiple suppliers, including ProSpec-Tany TechnoGene Ltd., East Brunswick, NJ, USA (Cat. No. CYT-230-b) and ThermoFisher Scientific, Inc., Waltham, MA, USA (human IL-15 recombinant protein, Cat. No. 34-8159-82). The amino acid sequence of recombinant human IL-15 suitable for use in die invention is given in Table 2 (SEQ ID NO: 11).

[0748] The term “IL-21” (also referred to herein as “IL21”) refers to the pleiotropic cytokine protein known as interleukin-21, and includes all forms of IL-21 including human and mammalian forms, conservative amino acid substitutions, glycoforms, biosimilars, and variants thereof. IL-21 is described, e.g., in Spolski and Leonard, Nat. Rev. Drug. Disc. 2014, 13, 379-95, the disclosure of which is incorporated by reference herein. IL-21 is primarily produced by natural killer T cells and activated human CD4+T cells. Recombinant human IL-21 is a single, non-glycosylated polypeptide chain containing 132 amino acids with a molecular mass of 15.4 kDa. Recombinant human IL-21 is commercially available from multiple suppliers, including ProSpec-Tany TechnoGene Ltd., East Brunswick, NJ, USA (Cat. No. CYT-408-b) and ThermoFisher Scientific, Inc., Waltham, MA, USA (human IL-21 recombinant protein. Cat. No. 14-8219-80). The amino acid sequence of recombinant human IL-21 suitable for use in the invention is given in Table 2 (SEQ ID NO: 12).

[0749] When “an anti-tumor effective amount”, “a tumor-inhibiting effective amount”, or “therapeutic amount” is indicated, the precise amount of the compositions of the present invention to be administered can be determined by a physician with consideration of individual differences in age, weight, tumor size, extent of infection or metastasis, and condition of the patient (subject). It can generally be stated that a pharmaceutical composition comprising the tumor infiltrating lymphocytes (e.g., secondary TILs or genetically modified cytotoxic lymphocytes) described herein may be administered at a dosage of 104to 1011cells / kg body weight (e.g., 105to 106, 105to IO10, 105to 1011, 106to IO10, 106to 10n,107to 10n, 107to 1010, 108to 1011, 108to IO10, 109to 1011, or 109to 1010cells / kg body weight), including all integer values within those ranges. TILs (including in some cases, genetically modified cytotoxic lymphocytes) compositions may also be administered multiple times at these dosages. The TILs (inlcuding in some cases, genetically) can be administered by using infusion techniques that are commonly known in immunotherapy (see, e.g., Rosenberg et al., New Eng. J. of Med. 1988, 319: 1676). The optimal dosage and treatment regime for a particular patient can readily be determined by one skilled in the art of medicine by monitoring the patient for signs of disease and adjusting the treatment accordingly.

[0750] The term “hematological malignancy”, “hematologic malignancy” or terms of correlative meaning refer to mammalian cancers and tumors of the hematopoietic and lymphoid tissues, including but not limited to tissues of the blood, bone marrow, lymph nodes, and lymphatic system. Hematological malignancies are also referred to as “liquid tumors.” Hematological malignancies include, but are not limited to, acute lymphoblastic leukemia (ALL), chronic lymphocytic lymphoma (CLL), small lymphocytic lymphoma (SLL), acute myelogenous leukemia (AML), chronic myelogenous leukemia (CML), multiple myeloma, acute monocytic leukemia (AMoL), Hodgkin's lymphoma, and non-Hodgkin's lymphomas. The term “B cell hematological malignancy” refers to hematological malignancies that affect B cells.

[0751] The term “liquid tumor” refers to an abnormal mass of cells that is fluid in nature. Liquid tumor cancers include, but are not limited to, leukemias, myelomas, and lymphomas, as well as other hematological malignancies. TILs obtained from liquid tumors may also be referred to herein as marrow infiltrating lymphocytes (MILs). TILs obtained from liquid tumors, including liquid tumors circulating in peripheral blood, may also be referred to herein as PBLs. The terms MIL, TIL, and PBL are used interchangeably herein and differ only based on the tissue type from which the cells are derived.

[0752] The term “microenvironment,” as used herein, may refer to the solid or hematological tumor microenvironment as a whole or to an individual subset of cells within the microenvironment. The tumor microenvironment, as used herein, refers to a complex mixture of “cells, soluble factors, signaling molecules, extracellular matrices, and mechanical cues that promote neoplastic transformation, support tumor growth and invasion, protect the tumor from host immunity, foster therapeutic resistance, and provide niches for dominant metastases to thrive,” as described in Swartz, et al., Cancer Res., 2012, 72, 2473. Although tumors express antigens that should be recognized by T cells, tumor clearance by the immune system is rare because of immune suppression by the microenvironment.

[0753] In some embodiments, the invention includes a method of treating a cancer with a population of TILs, wherein a patient is pre-treated with non-myeloablative chemotherapy prior to an infusion of TILs according to the invention. In some embodiments, the population of TILs may be provided wherein a patient is pre-treated with nonmyeloablative chemotherapy prior to an infusion of TILs according to the present invention. In some embodiments, the non-myeloablative chemotherapy is cyclophosphamide 60 mg / kg / d for 2 days (days 27 and 26 prior to TIL infusion) and fludarabine 25 mg / m2 / d for 5 days (days 27 to 23 prior to TIL infusion). In some embodiments, after non- myeloablative chemotherapy and TIL infusion (at day 0) according to the invention, the patient receives an intravenous infusion of IL-2 intravenously at 720,000 lU / kg every 8 hours to physiologic tolerance.

[0754] Experimental findings indicate that lymphodepletion prior to adoptive transfer of tumorspecific T lymphocytes plays a key role in enhancing treatment efficacy by eliminating regulatory T cells and competing elements of the immune system (“cytokine sinks”). Accordingly, some embodiments of the invention utilize a lymphodepletion step (sometimes also referred to as “immunosuppressive conditioning”) on the patient prior to the introduction of the TILs of the invention.

[0755] The term “effective amount” or “therapeutically effective amount” refers to that amount of a compound or combination of compounds as described herein that is sufficient to effect the intended application including, but not limited to, disease treatment. A therapeutically effective amount mayvary depending upon the intended application (in vitro or in vivo), or the subject and disease condition being treated (e.g., the weight, age and gender of the subject), the severity of the disease condition, or the manner of administration. The term also applies to a dose that will induce a particular response in target cells (e.g., the reduction of platelet adhesion and / or cell migration). The specific dose will vary depending on the particular compounds chosen, the dosing regimen to be followed, whether the compound is administered in combination with other compounds, timing of administration, the tissue to which it is administered, and the physical delivery system in which the compound is carried.

[0756] The terms “treatment”, “treating”, “treat”, and the like, refer to obtaining a desired pharmacologic and / or physiologic effect. The effect may be prophy lactic in terms of completely or partially preventing a disease or symptom thereof and / or may be therapeutic in terms of a partial or complete cure for a disease and / or adverse effect attributable to the disease. “Treatment”, as used herein, covers any treatment of a disease in a mammal, particularly in a human, and includes: (a) preventing the disease from occurring in a subject which may be predisposed to the disease but has not yet been diagnosed as having it; (b) inhibiting the disease, i.e., arresting its development or progression; and (c) relieving the disease, i.e., causing regression of the disease and / or relieving one or more disease symptoms. “Treatment” is also meant to encompass delivery of an agent in order to provide for a pharmacologic effect, even in the absence of a disease or condition. For example, “treatment” encompasses delivery of a composition that can elicit an immune response or confer immunity in the absence of a disease condition, e.g., in the case of a vaccine.

[0757] As used herein, the term "immune checkpoint inhibitor (ICI) " has its general meaning in the art and refers to any compound inhibiting the function of an immune inhibitory checkpoint protein. As used herein the term "immune checkpoint protein" has its general meaning in the art and refers to a molecule that is expressed by T cells in that either turn up a signal (stimulatory checkpoint molecules) or turn down a signal (inhibitory checkpoint molecules). Immune checkpoint molecules are recognized in the art to constitute immune checkpoint pathways similar to the CTLA-4 and PD-1 dependent pathways (see e g.. Pardoll, 2012. Nature Rev Cancer 12:252-264; Mellman et ah, 2011. Nature 480:480- 489). Examples of inhibitory checkpoint molecules include A2AR, B7-H3, B7-H4, CD277, IDO, KIR, VISTA, PD-1, CTLA-4, LAG-3, HAVCR2 (TIM-3), Cish, TGF[L PKA, CBL-B, PPP2CA, PPP2CB, PTPN6, PTPN22, PDCD1 , BTLA, CD 160, TIGIT, TET2, BAFF (BR3), CD96, CRTAM, LAIR1, SIGLEC7, SIGLEC9, CD244, TNFRSF10B, TNFRSF10A, CASP8, CASP10, CASP3, CASP6, CASP7, FADD, FAS, SMAD2, SMAD3, SMAD4, SMAD10, SKI, SKIL, TGIF1, IL10RA, IL10RB, HMOX2, IL6R, IL6ST, EIF2AK4, CSK, PAG1, SIT1, FOXP3, PRDM1, BATF, GUCY1A2, GUCY1A3, GUCY1B2, GUCY1B3, TOX, SOCS1, ANKRD11, and BCOR. For example, immune checkpoint genes that may be silenced or inhibited in TILs of the present invention may be selected from the group comprising PD-1, CTLA-4, LAG-3, TIM-3, Cish, CBL-B, TIGIT,TET2, TGF0, and PKA. BAFF (BR3) is described in Bloom, et al., J. Immunother. , 2018, in press. According to another example, immune checkpoint genes that may be silenced or inhibited in TILs of the present invention may be selected from the group comprising PD-1, LAG-3, TIM-3, CTLA-4, TIGIT, TET2, CISH, TGF0R2, PRA, CBLB, BAFF (BR3), and combinations thereof.

[0758] Inhibition includes reduction of function and full blockade. Preferred immune checkpoint inhibitors are antibodies that specifically recognize immune checkpoint proteins. A number of immune checkpoint inhibitors arc known and in analogy of these known immune checkpoint protein inhibitors, alternative immune checkpoint inhibitors may be developed in the (near) future. The immune checkpoint inhibitors include peptides, antibodies, nucleic acid molecules and small molecules.

[0759] The term “heterologous” when used with reference to portions of a nucleic acid or protein indicates that the nucleic acid or protein comprises two or more subsequences that are not found in the same relationship to each other in nature. For instance, the nucleic acid is typically rccombinantly produced, having two or more sequences from unrelated genes arranged to make a new functional nucleic acid, e.g., a promoter from one source and a coding region from another source, or coding regions from different sources. Similarly, a heterologous protein indicates that the protein comprises two or more subsequences that are not found in the same relationship to each other in nature (e.g. , a fusion protein).

[0760] The terms “sequence identity,” “percent identity,” and “sequence percent identity” (or synonyms thereof, e.g., “99% identical”) in the context of two or more nucleic acids or polypeptides, refer to two or more sequences or subsequences that are the same or have a specified percentage of nucleotides or amino acid residues that are the same, when compared and aligned (introducing gaps, if necessary) for maximum correspondence, not considering any conservative amino acid substitutions as part of the sequence identity. The percent identity can be measured using sequence comparison software or algorithms or by visual inspection. Various algorithms and software are known in the art that can be used to obtain alignments of amino acid or nucleotide sequences. Suitable programs to determine percent sequence identity include for example the BLAST suite of programs available from the U.S. Government’s National Center for Biotechnology Information BLAST web site. Comparisons between two sequences can be carried using either the BLASTN or BLASTP algorithm. BLASTN is used to compare nucleic acid sequences, while BLASTP is used to compare amino acid sequences. ALIGN, ALIGN-2 (Genentech, South San Francisco, California) or MegAlign, available from DNASTAR, are additional publicly available software programs that can be used to align sequences. One skilled in the art can determine appropriate parameters for maximal alignment by particular alignment software. In certain embodiments, the default parameters of the alignment software are used.

[0761] As used herein, the term “variant” encompasses but is not limited to antibodies or fusion proteins which comprise an amino acid sequence which differs from the amino acid sequence of a reference antibody by way of one or more substitutions, deletions and / or additions at certain positions within or adjacent to the amino acid sequence of the reference antibody. The variant may comprise one or more conservative substitutions in its amino acid sequence as compared to the amino acid sequence of a reference antibody. Conservative substitutions may involve, e.g., the substitution of similarly charged or uncharged amino acids. The variant retains the ability to specifically bind to the antigen of the reference antibody. The term variant also includes pegylated antibodies or proteins.

[0762] By “tumor infiltrating lymphocytes” or “TILs” herein is meant a population of cells originally obtained as white blood cells that have left the bloodstream of a subject and migrated into a tumor. TILs include, but are not limited to, CD8+cytotoxic T cells (lymphocytes), Thl and Thl7 CD4+T cells, natural killer cells, dendritic cells and Ml macrophages. TILs include both primary and secondary TILs. “Primary TILs” are those that are obtained from patient tissue samples as outlined herein (sometimes referred to as “freshly harvested”), and “secondary TILs” are any TIL cell populations that have been expanded or proliferated as discussed herein, including, but not limited to bulk TILs, expanded TILs (“REP TILs”) as well as “reREP TILs” as discussed herein. reREP TILs can include for example second expansion TILs or second additional expansion TILs (such as, for example, those described in Step D of Figure 8, including TILs referred to as reREP TILs).

[0763] TILs can generally be defined either biochemically, using cell surface markers, or functionally, by their ability to infiltrate tumors and effect treatment. TILs can be generally categorized by expressing one or more of the following biomarkers: CD4, CD8, TCR u|L CD27, CD28, CD56, CCR7, CD45Ra, CD95, PD-1, and CD25. Additionally, and alternatively, TILs can be functionally defined by their ability to infiltrate solid tumors upon reintroduction into a patient. TILs may further be characterized by potency - for example, TILs may be considered potent if, for example, interferon (IFN) release is greater than about 50 pg / mL, greater than about 100 pg / mL, greater than about 150 pg / mL, or greater than about 200 pg / mL. TILs may be considered potent if, for example, interferon (IFNy) release is greater than about 50 pg / mL, greater than about 100 pg / mL, greater than about 150 pg / mL, or greater than about 200 pg / mL, greater than about 300 pg / mL, greater than about 400 pg / mL, greater than about 500 pg / mL, greater than about 600 pg / mL, greater than about 700 pg / mL, greater than about 800 pg / mL, greater than about 900 pg / mL, greater than about 1000 pg / mL.

[0764] The term “deoxyribonucleotide” encompasses natural and synthetic, unmodified and modified deoxyribonucleotides. Modifications include changes to the sugar moiety, to the base moiety and / or to the linkages between deoxyribonucleotide in the oligonucleotide.

[0765] The term “RNA” defines a molecule comprising at least one ribonucleotide residue. The term “ribonucleotide” defines a nucleotide with a hydroxyl group at the 2' position of a b-D- ribofuranose moiety. The term RNA includes double -stranded RNA, single-stranded RNA, isolated RNA such as partially purified RNA, essentially pure RNA, synthetic RNA, recombinantly produced RNA, as well as altered RNA that differs from naturally occurring RNA by the addition, deletion, substitution and / or alteration of one or more nucleotides. Nucleotides of the RNA molecules described herein may also comprise non-standard nucleotides, such as non-naturally occurring nucleotides or chemically synthesized nucleotides or deoxynucleotides. These altered RNAs can be referred to as analogs or analogs of naturally-occurring RNA.

[0766] The terms “pharmaceutically acceptable carrier” or “pharmaceutically acceptable excipient” are intended to include any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and inert ingredients. The use of such pharmaceutically acceptable carriers or pharmaceutically acceptable excipients for active pharmaceutical ingredients is well known in the art. Except insofar as any conventional pharmaceutically acceptable carrier or pharmaceutically acceptable excipient is incompatible with the active pharmaceutical ingredient, its use in therapeutic compositions of the invention is contemplated. Additional active pharmaceutical ingredients, such as other drugs, can also be incorporated into the described compositions and methods.

[0767] The terms “about” and “approximately” mean within a statistically meaningful range of a value. Such a range can be within an order of magnitude, preferably within 50%, more preferably within 20%, more preferably still within 10%, and even more preferably within 5% of a given value or range. The allowable variation encompassed by the terms “about” or “approximately” depends on the particular system under study, and can be readily appreciated by one of ordinary skill in the art. Moreover, as used herein, the terms “about” and “approximately” mean that dimensions, sizes, formulations, parameters, shapes and other quantities and characteristics are not and need not be exact, but may be approximate and / or larger or smaller, as desired, reflecting tolerances, conversion factors, rounding off, measurement error and the like, and other factors known to those of skill in the art. In general, a dimension, size, formulation, parameter, shape or other quantity or characteristic is “about” or “approximate” whether or not expressly stated to be such. It is noted that embodiments of very different sizes, shapes and dimensions may employ the described arrangements.

[0768] The transitional terms “comprising,” “consisting essentially of,” and “consisting of,” when used in the appended claims, in original and amended form, define the claim scope with respect to what unrecited additional claim elements or steps, if any, are excluded from the scope of tire claim(s). The term “comprising” is intended to be inclusive or open-ended and does not exclude any additional, unrecited element, method, step or material. The term “consisting of’ excludes any element, step ormaterial other than those specified in the claim and, in the latter instance, impurities ordinary associated with the specified material(s). The term “consisting essentially of’ limits the scope of a claim to the specified elements, steps or material(s) and those that do not materially affect the basic and novel characteristic(s) of the claimed invention. All compositions, methods, and kits described herein that embody the present invention can, in alternate embodiments, be more specifically defined by any of the transitional terms “comprising,” “consisting essentially of,” and “consisting of.”

[0769] The terms “antibody” and its plural form “antibodies” refer to whole immunoglobulins and any antigen-binding fragment (“antigen-binding portion”) or single chains thereof. An “antibody” further refers to a glycoprotein comprising at least two heavy (H) chains and two light (L) chains inter-connected by disulfide bonds, or an antigen-binding portion thereof. Each heavy chain is comprised of a heavy chain variable region (abbreviated herein as VH) and a heavy chain constant region. The heavy chain constant region is comprised of three domains, CHI, CH2 and CH3. Each light chain is comprised of a light chain variable region (abbreviated herein as VL) and a light chain constant region. The light chain constant region is comprised of one domain, CL. The VH and VL regions of an antibody may be further subdivided into regions of hypervariability, which are referred to as complementarity determining regions (CDR) or hypervariable regions (HVR), and which can be interspersed with regions that are more conserved, termed framework regions (FR). Each VH and VL is composed of three CDRs and four FRs, arranged from amino-terminus to carboxy -terminus in the following order: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4. The variable regions of the heavy and light chains contain a binding domain that interacts with an antigen epitope or epitopes. The constant regions of the antibodies may mediate the binding of the immunoglobulin to host tissues or factors, including various cells of the immune system (e g., effector cells) and the first component (Clq) of the classical complement system.

[0770] The term “antigen” refers to a substance that induces an immune response. In some embodiments, an antigen is a molecule capable of being bound by an antibody or a TCR if presented by major histocompatibility complex (MHC) molecules. The term “antigen”, as used herein, also encompasses T cell epitopes. An antigen is additionally capable of being recognized by the immune system. In some embodiments, an antigen is capable of inducing a humoral immune response or a cellular immune response leading to the activation of B lymphocytes and / or T lymphocytes. In some cases, this may require that the antigen contains or is linked to a Th cell epitope. An antigen can also have one or more epitopes (e g., B- and T-epitopes). In some embodiments, an antigen will preferably react, typically in a highly specific and selective manner, with its corresponding antibody or TCR and not with the multitude of other antibodies or TCRs which may be induced by other antigens.

[0771] The terms “monoclonal antibody,” “mAb,” “monoclonal antibody composition,” or their plural forms refer to a preparation of antibody molecules of single molecular composition. Amonoclonal antibody composition displays a single binding specificity and affinity for a particular epitope. Monoclonal antibodies specific to certain receptors can be made using knowledge and skill in the art of injecting test subjects with suitable antigen and then isolating hybridomas expressing antibodies having the desired sequence or functional characteristics. DNA encoding the monoclonal antibodies is readily isolated and sequenced using conventional procedures (e.g., by using oligonucleotide probes that are capable of binding specifically to genes encoding the heavy and light chains of the monoclonal antibodies). The hybridoma cells serve as a preferred source of such DNA. Once isolated, the DNA may be placed into expression vectors, which are then transfected into host cells such as E. coll cells, simian COS cells, Chinese hamster ovary (CHO) cells, or myeloma cells that do not otherwise produce immunoglobulin protein, to obtain the synthesis of monoclonal antibodies in the recombinant host cells. Recombinant production of antibodies will be described in more detail below.

[0772] The terms “antigen-binding portion” or “antigen-binding fragment” of an antibody (or simply “antibody portion” or “fragment”), as used herein, refers to one or more fragments of an antibody that retain the ability to specifically bind to an antigen. It has been shown that the antigenbinding function of an antibody can be performed by fragments of a full-length antibody. Examples of binding fragments encompassed within the term “antigen-binding portion” of an antibody include (i) a Fab fragment, a monovalent fragment consisting of the VL, VH, CL and CHI domains; (ii) a F(ab')2 fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region; (iii) a Fd fragment consisting of the VH and CHI domains; (iv) a Fv fragment consisting of the VL and VH domains of a single arm of an antibody, (v) a domain antibody (dAb) fragment (Ward, et al., Nature, 1989, 341, 544-546), which may consist of a VH or a VL domain; and (vi) an isolated complementarity' determining region (CDR). Furthermore, although the two domains of the Fv fragment, VL and VH, are coded for by separate genes, they can be joined, using recombinant methods, by a synthetic linker that enables them to be made as a single protein chain in which the VL and VH regions pair to form monovalent molecules known as single chain Fv (scFv); see, e.g., Bird, et al., Science 1988, 242, 423-426; and Huston, et al., Proc. Natl. Acad. Sci. USA 1988, 85, 5879-5883). Such scFv antibodies are also intended to be encompassed within the terms “antigen-binding portion” or “antigen-binding fragment” of an antibody. These antibody fragments are obtained using conventional techniques known to those with skill in the art, and the fragments are screened for utility in the same manner as are intact antibodies. In some embodiments, a scFv protein domain comprises a VH portion and a VL portion. A scFv molecule is denoted as either VL-L-VH if the VL domain is the N- terminal part of the scFv molecule, or as VH-L-VLif the VH domain is the N-tenninal part of the scFv molecule. Methods for making scFv molecules and designing suitable peptide linkers are described in U.S. Pat. No. 4,704,692, U.S. Pat. No. 4,946,778, R. Raag and M. Whitlow, “Single Chain Fvs.” FASEB Vol 9:73-80 (1995) and R. E. Bird and B. W. Walker, Single Chain Antibody VariableRegions, TIBTECH, Vol 9: 132-137 (1991), the disclosures of which are incorporated by reference herein.

[0773] The term “human antibody,” as used herein, is intended to include antibodies having variable regions in which both the framework and CDR regions are derived from human germline immunoglobulin sequences. Furthermore, if the antibody contains a constant region, the constant region also is derived from human germline immunoglobulin sequences. The human antibodies of the invention may include amino acid residues not encoded by human germline immunoglobulin sequences (e.g., mutations introduced by random or site-specific mutagenesis in vitro or by somatic mutation in vivo). The term “human antibody”, as used herein, is not intended to include antibodies in which CDR sequences derived from the germline of another mammalian species, such as a mouse, have been grafted onto human framework sequences.

[0774] The term “human monoclonal antibody” refers to antibodies displaying a single binding specificity which have variable regions in which both the framework and CDR regions are derived from human germline immunoglobulin sequences. In some embodiments, the human monoclonal antibodies are produced by a hybridoma which includes a B cell obtained from a transgenic nonhuman animal, e.g., a transgenic mouse, having a genome comprising a human heavy chain transgene and a light chain transgene fused to an immortalized cell.

[0775] The term “recombinant human antibody”, as used herein, includes all human antibodies that are prepared, expressed, created or isolated by recombinant means, such as (a) antibodies isolated from an animal (such as a mouse) that is transgenic or transchromosomal for human immunoglobulin genes or a hybridoma prepared therefrom (described further below), (b) antibodies isolated from a host cell transformed to express the human antibody, e.g., from a transfectoma, (c) antibodies isolated from a recombinant, combinatorial human antibody library, and (d) antibodies prepared, expressed, created or isolated by any other means that involve splicing of human immunoglobulin gene sequences to other DNA sequences. Such recombinant human antibodies have variable regions in which the framework and CDR regions are derived from human germline immunoglobulin sequences. In certain embodiments, however, such recombinant human antibodies can be subjected to in vitro mutagenesis (or, when an animal transgenic for human Ig sequences is used, in vivo somatic mutagenesis) and thus the amino acid sequences of the VH and VL regions of the recombinant antibodies are sequences that, while derived from and related to human germline VH and VL sequences, may not naturally exist within the human antibody germline repertoire in vivo.

[0776] As used herein, “isotype” refers to the antibody class (e.g., IgM or IgGl) that is encoded by the heavy chain constant region genes.

[0777] The phrases “an antibody recognizing an antigen” and “an antibody specific for an antigen’ are used interchangeably herein with the term “an antibody which binds specifically to an antigen.”

[0778] The term “human antibody derivatives” refers to any modified form of the human antibody , including a conjugate of the antibody and another active pharmaceutical ingredient or antibody . The terms “conjugate,” “antibody-drug conjugate”, “ADC,” or “immunoconjugate” refers to an antibody, or a fragment thereof, conjugated to another therapeutic moiety, which can be conjugated to antibodies described herein using methods available in the art.

[0779] The terms “humanized antibody,” “humanized antibodies,” and “humanized” are intended to refer to antibodies in which CDR sequences derived from the germline of another mammalian species, such as a mouse, have been grafted onto human framework sequences. Additional framework region modifications may be made within the human framework sequences. Humanized forms of nonhuman (for example, murine) antibodies are chimeric antibodies that contain minimal sequence derived from non-human immunoglobulin. For the most part, humanized antibodies are human immunoglobulins (recipient antibody) in which residues from a hypervariable region of the recipient are replaced by residues from a 15 hypervariable region of a non-human species (donor antibody) such as mouse, rat, rabbit or nonhuman primate having the desired specificity, affinity, and capacity. In some instances, Fv framework region (FR) residues of the human immunoglobulin are replaced by corresponding non-human residues. Furthermore, humanized antibodies may comprise residues that are not found in the recipient antibody or in the donor antibody. These modifications are made to further refine antibody performance. In general, the humanized antibody will comprise substantially all of at least one, and typically two, variable domains, in which all or substantially all of the hypervariable loops correspond to those of a non-human immunoglobulin and all or substantially all of the FR regions are those of a human immunoglobulin sequence. The humanized antibody optionally also will comprise at least a portion of an immunoglobulin constant region (Fc), typically that of a human immunoglobulin. For further details, see Jones, et al., Nature 1986, 321, 522-525; Riechmamr, et al., Nature 1988, 332, 323-329; and Presta, Curr. Op. Struct. Biol. 1992, 2, 593-596. The antibodies described herein may also be modified to employ any Fc variant which is known to impart an improvement (e.g., reduction) in effector function and / or FcR binding. The Fc variants may include, for example, any one of the amino acid substitutions disclosed in International Patent Application Publication Nos. WO 1988 / 07089 Al, WO 1996 / 14339 Al, WO 1998 / 05787 Al, WO 1998 / 23289 Al, WO 1999 / 51642 Al, WO 99 / 58572 Al, WO 2000 / 09560 A2, WO 2000 / 32767 Al, WO 2000 / 42072 A2, WO 2002 / 44215 A2, WO 2002 / 060919 A2, WO 2003 / 074569 A2, WO 2004 / 016750 A2, WO 2004 / 029207 A2, WO 2004 / 035752 A2, WO 2004 / 063351 A2, WO 2004 / 074455 A2, WO 2004 / 099249 A2, WO 2005 / 040217 A2, WO 2005 / 070963 Al, WO 2005 / 077981 A2, WO 2005 / 092925 A2, WO 2005 / 123780 A2, WO 2006 / 019447 Al, WO2006 / 047350 A2, and WO 2006 / 085967 A2; and U.S. Patent Nos. 5,648,260; 5,739,277; 5,834,250;5,869,046; 6,096,871; 6,121,022; 6,194,551; 6,242,195; 6,277,375; 6,528,624; 6,538,124; 6,737,056;6,821,505; 6,998,253; and 7,083,784; the disclosures of which are incorporated by reference herein.

[0780] The term “chimeric antibody” is intended to refer to antibodies in which the variable region sequences are derived from one species and the constant region sequences are derived from another species, such as an antibody in which the variable region sequences are derived from a mouse antibody and the constant region sequences are derived from a human antibody.

[0781] A “diabody” is a small antibody fragment with two antigen-binding sites. The fragments comprises a heavy chain variable domain (VH) connected to a light chain variable domain (VL) in the same polypeptide chain (VH-VL or VL-VH). By using a linker that is too short to allow pairing between the two domains on the same chain, the domains are forced to pair with the complementary domains of another chain and create two antigen-binding sites. Diabodies are described more fully in, e.g., European Patent No. EP 404,097, International Patent Publication No. WO 93 / 11161; and Bolliger, et al., Proc. Natl. Acad. Set. USA 1993, 90, 6444-6448.

[0782] The term “glycosylation” refers to a modified derivative of an antibody. An aglycoslated antibody lacks glycosylation. Glycosylation can be altered to, for example, increase the affinity of the antibody for antigen. Such carbohydrate modifications can be accomplished by, for example, altering one or more sites of glycosylation within the antibody sequence. For example, one or more amino acid substitutions can be made that result in elimination of one or more variable region framework glycosylation sites to thereby eliminate glycosylation at that site. Aglycosylation may increase the affinity of the antibody for antigen, as described in U.S. Patent Nos. 5,714,350 and 6,350,861. Additionally or alternatively, an antibody can be made that has an altered type of glycosylation, such as a hypofucosylated antibody having reduced amounts of fucosyl residues or an antibody having increased bisecting GlcNac structures. Such altered glycosylation patterns have been demonstrated to increase the ability of antibodies. Such carbohydrate modifications can be accomplished by, for example, expressing the antibody in a host cell with altered glycosylation machinery. Cells with altered glycosylation machinery have been described in the art and can be used as host cells in which to express recombinant antibodies of the invention to thereby produce an antibody with altered glycosylation. For example, the cell lines Ms704, Ms705, and Ms709 lack the fucosyltransferase gene, FUT8 (alpha (1,6) fucosyltransferase), such that antibodies expressed in the Ms704, Ms705, and Ms709 cell lines lack fucose on their carbohydrates. The Ms704, Ms705, and Ms709 FUT8- / - cell lines were created by the targeted disruption of the FUT8 gene in CHO / DG44 cells using two replacement vectors (see e.g. U.S. Patent Publication No. 2004 / 0110704 or Yamane-Ohnuki, et al., Biotechnol. Bioeng., 2004, 87, 614-622). As another example, European Patent No. EP 1,176,195 describes a cell line with a functionally disrupted FUT8 gene, which encodes a fucosyl transferase,such that antibodies expressed in such a cell line exhibit hypofucosylation by reducing or eliminating the alpha 1,6 bond-related enzyme, and also describes cell lines which have a low enzyme activity for adding fucose to the N-acetylglucosamine that binds to the Fc region of the antibody or does not have the enzyme activity, for example the rat myeloma cell line YB2 / 0 (ATCC CRL 1662). International Patent Publication WO 03 / 035835 describes a variant CHO cell line, Lee 13 cells, with reduced ability to attach fucose to Asn(297) -linked carbohydrates, also resulting in hypofucosylation of antibodies expressed in that host cell (see also Shields, et al., J. Biol. Chem. 2002, 277, 26733-26740. International Patent Publication WO 99 / 54342 describes cell lines engineered to express glycoproteinmodifying glycosyl transferases (e.g., beta(l,4)-N-acetylglucosaminyltransferase III (GnTIII)) such that antibodies expressed in the engineered cell lines exhibit increased bisecting GlcNac structures which results in increased ADCC activity of the antibodies (see also Umana, et al., Nat. Biotech.1999, 17, 176-180). Alternatively, the fucose residues of the antibody may be cleaved off using a fucosidase enzyme. For example, the fucosidase alpha-L-fucosidase removes fucosyl residues from antibodies as described in Tarentino, et al. , Biochem. 1975, 14, 5516-5523.

[0783] “Pegylation” refers to a modified antibody, or a fragment thereof, that typically is reacted with polyethylene glycol (PEG), such as a reactive ester or aldehyde derivative of PEG, under conditions in which one or more PEG groups become attached to the antibody or antibody fragment. Pegylation may, for example, increase the biological (e.g., serum) half life of the antibody Preferably, the pegylation is carried out via an acylation reaction or an alkylation reaction with a reactive PEG molecule (or an analogous reactive water-soluble polymer). As used herein, the term “poly ethylene glycol” is intended to encompass any of the forms of PEG that have been used to derivatize other proteins, such as mono (Ci-Cio)alkoxy- or aryloxy-polyethylene glycol or polyethylene glycol- maleimide. The antibody to be pegylated may be an aglycosylated antibody. Methods for pegylation are known in the art and can be applied to the antibodies of the invention, as described for example in European Patent Nos. EP 0154316 and EP 0401384 and U.S. Patent No. 5,824,778, the disclosures of each of which are incorporated by reference herein.

[0784] The term “biosimilar” means a biological product, including a monoclonal antibody or protein, that is highly similar to a U.S. licensed reference biological product notwithstanding minor differences in clinically inactive components, and for which there are no clinically meaningful differences between the biological product and tire reference product in terms of the safety, purity, and potency of the product. Furthermore, a similar biological or “biosimilar” medicine is a biological medicine that is similar to another biological medicine that has already been authorized for use by the European Medicines Agency. The term “biosimilar” is also used synonymously by other national and regional regulatory agencies. Biological products or biological medicines are medicines that are made by or derived from a biological source, such as a bacterium or yeast. They can consist of relativelysmall molecules such as human insulin or erythropoietin, or complex molecules such as monoclonal antibodies. For example, if the reference IL-2 protein is aldesleukin (PROLEUKIN), a protein approved by drug regulatory authorities with reference to aldesleukin is a “biosimilar to” aldesleukin or is a “biosimilar thereof’ of aldesleukin. In Europe, a similar biological or “biosimilar” medicine is a biological medicine that is similar to another biological medicine that has already been authorized for use by the European Medicines Agency (EMA). The relevant legal basis for similar biological applications in Europe is Article 6 of Regulation (EC) No 726 / 2004 and Article 10(4) of Directive 2001 / 83 / EC, as amended and therefore in Europe, the biosimilar may be authorized, approved for authorization or subject of an application for authorization under Article 6 of Regulation (EC) No 726 / 2004 and Article 10(4) of Directive 2001 / 83 / EC. The already authorized original biological medicinal product may be referred to as a “reference medicinal product” in Europe. Some of the requirements for a product to be considered a biosimilar are outlined in the CHMP Guideline on Similar Biological Medicinal Products. In addition, product specific guidelines, including guidelines relating to monoclonal antibody biosimilars, are provided on a product-by-product basis by the EMA and published on its website. A biosimilar as described herein may be similar to the reference medicinal product by way of quality characteristics, biological activity, mechanism of action, safety profiles and / or efficacy. In addition, the biosimilar may be used or be intended for use to treat the same conditions as the reference medicinal product. Thus, a biosimilar as described herein may be deemed to have similar or highly similar quality characteristics to a reference medicinal product. Alternatively, or in addition, a biosimilar as described herein may be deemed to have similar or highly similar biological activity to a reference medicinal product. Alternatively, or in addition, a biosimilar as described herein may be deemed to have a similar or highly similar safety profile to a reference medicinal product. Alternatively, or in addition, a biosimilar as described herein may be deemed to have similar or highly similar efficacy to a reference medicinal product. As described herein, a biosimilar in Europe is compared to a reference medicinal product which has been authorized by the EMA. However, in some instances, the biosimilar may be compared to a biological medicinal product which has been authorized outside the European Economic Area (a non-EEA authorized “comparator”) in certain studies. Such studies include for example certain clinical and in vivo non- clinical studies. As used herein, the term “biosimilar” also relates to a biological medicinal product which has been or may be compared to a non-EEA authorized comparator. Certain biosimilars are proteins such as antibodies, antibody fragments (for example, antigen binding portions) and fusion proteins. A protein biosimilar may have an amino acid sequence that has minor modifications in the amino acid structure (including for example deletions, additions, and / or substitutions of amino acids) which do not significantly affect the function of the polypeptide. The biosimilar may comprise an amino acid sequence having a sequence identity of 97% or greater to the amino acid sequence of its reference medicinal product, e.g., 97%, 98%, 99% or 100%. The biosimilar may comprise one ormore post-translational modifications, for example, although not limited to, glycosylation, oxidation, deamidation, and / or truncation which is / are different to the post-translational modifications of the reference medicinal product, provided that the differences do not result in a change in safety and / or efficacy of the medicinal product. The biosimilar may have an identical or different glycosylation pattern to the reference medicinal product. Particularly, although not exclusively, the biosimilar may have a different glycosylation pattern if the differences address or are intended to address safety concerns associated with the reference medicinal product. Additionally, the biosimilar may deviate from the reference medicinal product in for example its strength, pharmaceutical form, formulation, excipients and / or presentation, providing safety and efficacy of the medicinal product is not compromised. The biosimilar may comprise differences in for example pharmacokinetic (PK) and / or pharmacodynamic (PD) profdes as compared to the reference medicinal product but is still deemed sufficiently similar to the reference medicinal product as to be authorized or considered suitable for authorization. In certain circumstances, the biosimilar exhibits different binding characteristics as compared to the reference medicinal product, wherein the different binding characteristics are considered by a Regulatory Authority such as the EMA not to be a barrier for authorization as a similar biological product. The term “biosimilar” is also used synonymously by other national and regional regulatory agencies.III. Making a cryopreserved TIL preparation from tumor harvest

[0785] Some emboidments of the present invention provided herein are directed to methods of making a cryopreserved TIL preparation from a tumor sample harvested from a cancer patient before the patient receives immune-check point inhibitor (ICI) and / or standard of care treatment.

[0786] In some embodiments, the patient receives ICI treatment (such as anti-immune checkpoint antibodies as described herein) after the tumor harvest and cryopreservation.

[0787] In some embodiments, the patient receives standard of care treatment for cancer after the tumor harvest and cry opreservation. Several of the methods of treatment described herein comprise the administration of a standard of care treatment to a patient. As used herein, a “standard of care treatment” is a treatment process, including a drug or combination of drugs, radiation therapy, surgery or other medical intervention that is recognized by medical practitioners as appropriate, accepted, and / or widely used for a certain type of patient, disease or clinical circumstance. Standard of care treatments for treating different types of cancer are well known by persons of skill in the art. For example, the National Comprehensive Cancer Network (NCCN), an alliance of 21 major cancer centers in the USA, publishes the NCCN Clinical Practice Guidelines in Oncology (NCCN GUIDELINES®) that provide detailed up-to-date information on the standard-of-care treatments for awide variety of cancers (see NCCN GUIDELINES®, 2013). In some embodiments, the standard of care treatment is chemotherapy, radiation therapy, surgery, targeted therapy, or any combinations thereof.

[0788] In some embodiments, the cryopreserved tumor harvest from the patient or subject is used to make a population of TILs subsequent to the patient is treated with an ICI and / or standard of care treatment and shows progression of the cancer. In some embodiments, after the cry opreserving of the tumor harvest, the patient or subject receives immune-check point inhibitor (ICI) and / or standard of care treatment and is monitored for progression of the cancer. In some embodiments, the progression of the cancer is indicative of the need of an autologous TIL therapy.

[0789] In other embodiments, the cryopreserving of the tumor harvest from the patient or subject is completed prior to the progression of the cancer.

[0790] In some embodiments, the methods disclosed herein provide a pharmacoeconomic advantage in the form of avoiding the cost of TIL production in the event that the paetint does not develop progressive sdisease or is otherwise indicated for TIL therapy in the future.

[0791] The method of any one of claims 1 to 14, wherein the patient exhibits progressive disease at least about 1 week, 2 weeks, 3 weeks, 1 month, 2 months, 3 month, 4 months, 5 months, 6 months, 7 months, 8 months, 9 months, 10 months, 11 months, 12 months, 13 months, 14 months, 15 months, 16 months, 17 months, 18 months, 19 months, 20 months, 21 months, 22 months, 23 months, 24 months, 25 months, 26 months, 1 months, 28 months, 29 months, 30 months, 31 months, 32 months, 33 months, 34 months, 35 months, 36 months, after the step of cry opreserving.

[0792] In some embodiments, the cryopreserved TIL preparation can be made from a tumor sample comprising a population of TILs that is obtained and / or received from the patient or subject. A patient tumor sample may be obtained using methods known in the art, generally via surgical resection, needle biopsy, core biopsy, small biopsy, or other means for obtaining a sample that contains a mixture of tumor and TIL cells. In some embodiments, multilesional sampling is used. In some embodiments, surgical resection, needle biopsy, core biopsy, small biopsy, or other means for obtaining a sample that contains a mixture of tumor and TIL cells includes multilesional sampling (i.e., obtaining samples from one or more tumor sites and / or locations in the patient, as well as one or more tumors in the same location or in close proximity). In general, the tumor sample may be from any solid tumor, including primary tumors, invasive tumors or metastatic tumors. The tumor sample may also be a liquid tumor, such as a tumor obtained from a hematological malignancy. The solid tumor may be of lung tissue. In some embodiments, useful TILs are obtained from non-small cell lung carcinoma (NSCLC). The solid tumor may be of skin tissue. In some embodiments, useful TILs are obtained from a melanoma.

[0793] Once obtained, the tumor sample is generally fragmented using sharp dissection into small pieces of between 1 to about 8 mm3, with from about 2-3 mm3being particularly useful. In some embodiments, the TILs are cultured from these fragments using enzymatic tumor digests. Such tumor digests may be produced by incubation in enzymatic media (e.g., Roswell Park Memorial Institute (RPMI) 1640 buffer, 2 mM glutamate, 10 mcg / mL gentamicine, 30 units / mL of DNase and 1.0 mg / mL of collagenase) followed by mechanical dissociation (e.g., using a tissue dissociator). Tumor digests may be produced by placing the tumor in enzymatic media and mechanically dissociating the tumor for approximately 1 minute, followed by incubation for 30 minutes at 37 °C in 5% CO2, followed by repeated cycles of mechanical dissociation and incubation under the foregoing conditions until only small tissue pieces are present. At the end of this process, if the cell suspension contains a large number of red blood cells or dead cells, a density gradient separation using FICOLL branched hydrophilic polysaccharide may be performed to remove these cells. Alternative methods known in the art may be used, such as those described in U.S. Patent Application Publication No. 2012 / 0244133 Al, the disclosure of which is incorporated by reference herein. Any of the foregoing methods may be used in any of the embodiments described herein for methods of expanding TILs or methods treating a cancer.

[0794] In some embodiments, the cryopreserved TIL preparation is stored for future use. In some embodiments, the cry opreservation of the tumor harvest is completed pre-progression. In some embodiments, after the making of the cryopreserved TIL preparation, the patient is monitored for exhibitions of progressive disease on or after ICI and / or standard of care treatment. In some embodiments, the patient exhibits progressive cancer on or after ICI and / or standard of care treatment and is indicated for autologous TIL therapy. In some embodiments, after progression, the cryopreserved TIL preparation is thawed and expanded according to expansion methods described in the sections below.

[0795] In some embodiments, the patient is a NSCLC cancer patient. In some embodiments, the patient is suffering from, suffered from, or is prone to NSCLC. In some embodiments, the patient has metastatic NSCLC. In some embodiments, the patient has metastatic stage IV NSCLC.

[0796] In some emboidments, the subject or patient has at least one of: i. a predetermined tumor proportion score (TPS) of PD-L1 of < 1%, ii. a TPS score of PD-L1 of l%-49%, or iii. a predetermined absence of one or more driver mutations.

[0797] In some embodiments, the NSCLC patient is without one or more actionable driver mutations. In some embodiments, the actionable driver mutations disclosed herein include but are notlimited to an EGFR mutation, an EGFR insertion, EGFR exon20, a KRAS mutation, a BRAF- mutation, a BRAF V600E mutation, a BRAF V600K mutation, a BRAF V600 mutation, an ALK mutation, a c-ROS mutation (RO SI -mutation), a ROS1 fusion, a RET mutation, a RET fusion, an ERBB2 mutation, an ERBB2 amplification, a BRCA mutation, a MAP2K1 mutation, PIK3CA, CDKN2A, a PTEN mutation, an UMD mutation, an NRAS mutation, a KRAS mutation, an NF1 mutation, a MET mutation, a MET splice and / or altered MET signaling, a TP53 mutation, a CREBBP mutation, a KMT2C mutation, a KMT2D mutation, an ARID 1 A mutation, a RBI mutation, an ATM mutation, a SETD2 mutation, a FLT3 mutation, a PTPN11 mutation, a FGFR1 mutation, an EP300 mutation, a MYC mutation, an EZH2 mutation, a JAK2 mutation, a FBXW7 mutation, a CCND3 mutation, and a GNA11 mutation. In some embodiments, the NSCLC exhibits a TPS of < 1% and has a predetermined absence of one or more driver mutations.

[0798] In some embodiments, at the time the cryopreserved TIL preparation is made, the patient is naive to all cancer treatment. In some embodiments, the patient is naive to targeted therapies. In some embodiments, at the time the cryopreserved TIL preparation is made, the patient is naive to ICI treatment. In some embodiments, at the time the cryopreserved TIL preparation is made, the patient is naive to anti-VEGF (e g. avastin / bevacizumab) treatment. In some embodiments, at the time the cryopreserved TIL preparation is made, the patient is naive to chemotherapy treatment. In some embodiments, at the time the cryopreserved TIL preparation is made, the patient is naive to a combination of two or more of the foregoing treatments.

[0799] In some embodiments, at the time the cryopreserved TIL preparation is made, the patient is on maintenance therapy. In some embodiments, at the time the cryopreserved TIL preparation is made, the patient’s maintenance therapy is interrupted. In some embodiments, at the time the cryopreserved TIL preparation is made, the patient is in a washout period following the interruption of their maintenance therapy, followed by the resumption of the maintenance therapy or a different therapy. In some embodiments, the maintenance therapy will resume after a tumor sample is harvested from the patient. In some embodiments, the cancer (such as NSCLC) processes on or after the maintenance therapy.

[0800] In some embodiments, at the time the cryopreserved TIL preparation is made, the patient is going to receive first-line (IL) ICI and / or standard therapy for cancer.

[0801] In some embodiments, at the time the cryopreserved TIL preparation is made, the patient is going to receive second-line (2L) ICI and / or standard therapy for cancer.

[0802] In some embodiments, the harvested tumor sample of the patient is cryopreserved using flash-freezing methods or controlled rate freezing. Exemplary flash-freezing methods and controlledrate freezing methods can be found in International Patent Publication No. WO / 2020 / 061429, which is incorporated herein by reference in its entirety for all purposes.

[0803] In some embodiments, the flash-freezing methods of the present invention comprise:(i) fragmenting the tumor tissue;(ii) incubating the fragments in a cry opreservation medium; and,(iii) freezing the fragments wherein the freezing is flash freezing using the vapor phase of liquid nitrogen.

[0804] In an embodiment, the tumor tissue is fragmented into approximately spherical fragments having a diameter of about 1.5 mm to about 6 mm. In a preferred embodiment, the approximately spherical fragments have a diameter of about 6 mm. In an embodiment, the approximately spherical fragments have a diameter of about 3 mm.

[0805] In some embodiments, the tumor tissue is fragmented into generally rectangular fragments having a shortest edge length of at least 1.5 mm and a longest edge length of about 6 mm. In an embodiment, the tumor tissue is fragmented into generally cubical fragments having edge lengths of between about 1.5 mm and 6 mm. In an embodiment, the generally cubical fragments have edge lengths of about 6 mm. In an embodiment, the generally cubical fragments have edge lengths of about 3 mm.

[0806] In some embodiments, the tissue sample is trimmed to separate non-tumor tissue from tumor tissue.

[0807] In some embodiments, the tumor tissue is from a dissected tumor. In an embodiment, the tumor tissue is from a tumor biopsy. In some embodiments the tumor tissue is from an incisional biopsy. In some embodiments the tumor tissue is from an excisional biopsy. In some embodiments the tumor tissues may be from one or more core needle biopsies.

[0808] In some embodiments, the fresh tumor tissue is trimmed into fragments with a cross section of about 1.5 mm * 1.5 mm, about 2 mm x 2 mm, about 2.5 mm x 2.5 mm, about 3 mm x 3 mm, about 3.5 mm x 3.5 mm, about 4 mm x 4 mm, about 4.5 mm x 4.5 mm, about 5 mm x 5 mm, about 5.5 mm x about 5.5 mm, or about 6 mm x about 6 mm.

[0809] In an embodiment, the tumor tissue is less than twelve hours old. In an embodiment, the tumor tissue is less than eight hours old. In an embodiment, the tumor tissue is less than three, less than two, or less than one-hour old.

[0810] Any suitable cryopreservation medium known to those skilled in the art in view of the present disclosure can be used in the methods described herein. Examples of suitable cry opreservation mediums include, but are not limited to, CryoStor® CS10, HypoThermosol®, or a combination thereof. In some embodiments, the cry opreservation medium comprises about 2% v / v DMSO to about 15% v / v DMSO. In some embodiments, the cry opreservation medium comprises about 2% v / v DMSO. In some embodiments, the cry opreservation medium comprises about 2% v / v DMSO. In some embodiments, the crvoprcscrvaiion medium comprises about 3% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 4% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 5% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 6% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 7% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 8% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 9% v / v DMSO. In some embodiments, the cry opreservation medium comprises about 10% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 11% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 12% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 13% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 14% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 15% v / v DMSO. In some embodiments, the cryopreservation medium comprises at least one antimicrobial agent. Any suitable antimicrobial agent known to those skilled in the art in view of the present disclosure can be used in the methods described herein. In some embodiments, the cryopreservation medium comprises gentamicin. In some embodiments, the cry opreservation medium comprises gentamicin at a concentration of at least 50 pg / mL. In some embodiments, the cry opreservation medium comprises gentamicin at a concentration of at least 40 pg / mL. In some embodiments, the cryopreservation medium comprises gentamicin at a concentration of at least 30 pg / mL. In some embodiments, the cryopreservation medium comprises gentamicin at a concentration of at least 20 pg / mL.

[0811] In some embodiments, the tumor fragments are incubated in cry opreservation medium for about 20 minutes to about 70 minutes. In some embodiments, the tumor fragments are incubated in cryopreservation medium for about 30 minutes to about 60 minutes. In some embodiments the incubation is at least 10 minutes; at least 20 minutes; at least 25 minutes; at least 30 minutes; at least 35 minutes; at least 40 minutes; at least 45 minutes; at least 50 minutes; at least 55 minutes; at least 60 minutes; or at least 70 minutes. In some embodiments the incubation is about 10 minutes; about 20 minutes; about 25 minutes; about 30 minutes; about 35 minutes; about 40 minutes; about 45 minutes; about 50 minutes; about 55 minutes; about 60 minutes; or about 70 minutes. In some embodiments the incubation is less than 10 minutes; less than 20 minutes; less than 25 minutes; less than 30minutes; less than 35 minutes; less than 40 minutes; less than 45 minutes; less than 50 minutes; less than 55 minutes; less than 60 minutes; or less than 70 minutes. In some embodiments the incubation time is proportional to tumor fragment density. In some embodiments the incubation time is proportional to tumor fragment surface to volume ratio.

[0812] In some embodiments, the tumor fragments are incubated in a cryopreser ation medium at a temperature from about 2°C to about 8°C.

[0813] In some embodiments, the tumor tissue is washed in a physiologically buffered isotonic saline solution. In some embodiments, the washing comprises three serial washes of at least three minutes each, with the physiologically buffered isotonic saline solution replaced after each serial wash. In some embodiments, the physiologically buffered isotonic saline solution comprises Hank’s Balance Salt Solution (HBSS). In some embodiments, the physiologically buffered isotonic saline solution comprises tris-buffered saline (TBS). In some embodiments, the physiologically buffered isotonic saline solution comprises phosphate buffered saline (PBS). In some embodiments, the physiologically buffered isotonic saline solution comprises Dulbecco's phosphate-buffered saline (DPBS). In some embodiments, the physiologically buffered isotonic saline solution in one serial wash may be a different physiologically buffered isotonic saline solution than used in one or more of the other serial washes.

[0814] In an embodiment, the freezing takes place at a temperature in the range from about - 125°C to about -196°C. In an embodiment, the freezing takes place at a temperature in the range of about -140°C to about -185°C. In an embodiment, the freezing takes place at a temperature in the range of about -140°C to about -175°C. In an embodiment, the freezing takes place at a temperature of about -145°C. In some embodiments, the freezing takes place in the vapor phase of liquid nitrogen.

[0815] A problem well-known in the art is to cryopreserve cells or tissues without damaging them during the freezing process. Without being bound by theory, one source of damage during freezing is intracellular ice nucleation resulting in cellular rupture. Muldrew and McGann outline a quantitative theory for this well-known and widely recognized difficulty with cellular and, in particular, whole tissue cryopreservation in “The osmotic rupture hypothesis of intracellular freezing injury”, Biophysical Journal, 66:532-41 (1994). Acker and McGann further develop the fundamental mechanisms of intracellular ice formation and cellular damage in a later article, “Membrane damage occurs during the formation of intracellular ice,” Cryo Letter, 22:241-54 (2001).

[0816] Damage during freezing is detected wherein upon drawing, the tissues have substantially lost their physiological structures or the cells comprising the tissue have substantially lost their viabilit . Viability may be determined by the fraction of cells introduced into a culture medium compared to the number of such cells that grow or show markers of normal cellular function.Numerous methods are known in the art to identify the fraction of viable cells, for example, and without limitation, dye exclusion tests, such as trypan blue exclusion. See, for example, Strober, Curr. Protoc. Immunol., 2001, Appendix 3B, available at https: / / dx.doi.org / 10.1002 / 0471142735.hna03bs21. Without limitation, viability may also be determined by metabolic activity assays, such as the MTT assay, wherein MTT, 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, is metabolized by cellular enzymes into formazan. This enzymatic reaction converts the yellow MTT into purple formazan. See, for example, Berridge et al., Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnology Annual Review, 11: 127-152 (2005); Mosmann, “Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays,” J. Immunol. Methods 65 (1- 2): 55-63 (1983).

[0817] Without being bound by theory, slow cooling is hypothesized to produce innocuous intracellular ice, see Acker and McGann, “Protective effect of intracellular ice during freezing?” Cryobiology, 46(2): 197-202 (2003). A conventional approach to achieve freezing without excessive cellular damage or to achieve freezing without significantly reducing cellular viability has been to employ slow freezing rates. Watson et al., U.S. Patent 5,891,617, emphasize this approach, for example claim one, step (c), teaches a very slow rate of cooling: “about -0.3° C. per minute or less.” Similarly, Comhaire et al., U.S. Patent 9,938,495, teach “high cell viability after thawing were obtained by slow freezing using a DMSO-free cryopreservation medium” for stem cells.

[0818] Based on these exemplary teachings, the methods and products of the present disclosure are surprising and unexpected. Further the present disclosure, including the examples and the data therein, demonstrate a technical solution to the problem of rapidly and efficiently cryoprotecting tumor tissues, tumor fragments, or tumor specimens, for the use in the manufacture of tumor infiltrating lymphocytes for therapeutic use.

[0819] In some embodiments, the method of cryopreserving tumor tissue for the manufacture of tumor infiltrating lymphocytes (TILs), further comprises a step (iv) storing the frozen fragments at a temperature below at least -130°C. In some embodiments, the frozen fragments are stored in the vapor phase of liquid nitrogen. In some embodiments, the frozen fragments are stored submerged in liquid nitrogen. In some embodiments, the cryopreserved fragments are stored for later manufacture of TILs for autologous therapeutic use.

[0820] In some embodiments, the disclosures provide herein methods for cryopreserving tumor tissue using controlled-rate freezing / slow-freezing methods.

[0821] In some embodiments, the present invention provides a method for cry opreserving tumor tissue, and a cryopreserved tumor tissue prepared by a process comprising the steps of:(i) adding cryopreservation medium to a closable vessel;(ii) pre-cooling the closable vessel in a controlled-rate freezing device;(iii) fragmenting tumor tissue to obtain tumor fragments;(iv) placing the tumor fragments in the closable vessel comprising cry opreservation medium and closing the vessel;(v) optionally incubating the closed vessel comprising the tumor fragments and cryopreservation medium;(vi) slow-freezing the vessel in a controlled-rate freezing device; and(vii) transferring the vessel to a liquid nitrogen freezer.

[0822] In some embodiments, the present invention provides a method for cry opreserving tumor tissue, and a cryopreserved tumor tissue prepared by a process comprising the steps of:(i) placing in a pre-cooled closable vessel comprising cryoprcscrvation medium tumor fragments obtained from fragmenting tumor tissue and closing the vessel;(ii) optionally incubating the closed vessel comprising the tumor fragments and cryopreservation medium;(iii) slow-freezing the vessel in a controlled-rate freezing device; and(iv) transferring the vessel to a liquid nitrogen freezer.

[0823] In some embodiments, the present invention provides a method for cry opreserving tumor tissue, and a cryopreserved tumor tissue prepared by a process comprising the steps of:(i) placing in a pre-cooled closable vessel comprising cryoprcscrvation medium a tumor digest obtained from digesting in an enzymatic media tumor tissue or tumor fragments produced from fragmenting tumor tissue and closing the vessel;(ii) optionally incubating the closed vessel comprising the tumor digest and cry opreservation medium;(iii) slow-freezing the vessel in a controlled-rate freezing device; and(iv) transferring the vessel to a liquid nitrogen freezer.

[0824] In some embodiments, the present invention provides a method for cry opreserving tumor tissue, and a cryopreserved tumor tissue prepared by a process comprising the steps of:(i) adding cryopreservation medium to a closable vessel;(ii) pre-cooling the closable vessel in a controlled-rate freezing device;(iii) digesting tumor tissue in an enzymatic media to obtain a tumor digest;(iv) placing the tumor digest in the cry opreservation medium in the closable vessel and closing the vessel;(v) optionally incubating the closed vessel comprising the tumor digest and cryopreservation medium;(vi) slow-freezing the vessel in a controlled-rate freezing device; and(vii) transferring the vessel to a liquid nitrogen freezer.

[0825] Any suitable cryopreservation medium known to those skilled in the art in view of the present disclosure can be used in the methods described herein. Examples of suitable cryopreservation mediums include, but are not limited to, CryoStor® CS10, HypoThermosol®, or a combination thereof. In some embodiments, the cry opreservation medium comprises about 2% v / v DMSO to about 15% v / v DMSO In some embodiments, the cry opreservation medium comprises about 2% v / v DMSO. In some embodiments, the cry opreservation medium comprises about 2% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 3% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 4% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 5% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 6% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 7% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 8% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 9% v / v DMSO. In some embodiments, the cry opreservation medium comprises about 10% v / v DMSO. In some embodiments, the cry opreservation medium comprises about 11% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 12% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 13% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 14% v / v DMSO. In some embodiments, the cryopreservation medium comprises about 15% v / v DMSO. In some embodiments, the cryopreservation medium comprises at least one antimicrobial agent. Any suitable antimicrobial agent known to those skilled in the art in view of the present disclosure can be used in the methods described herein. In some embodiments, the cryopreservation medium comprises gentamicin. In some embodiments, the cry opreservation medium comprises gentamicin at a concentration of at least 50 pg / mL. In some embodiments, the cry opreservation medium comprises gentamicin at a concentrationIllof at least 40 pg / mL. In some embodiments, tire cryopreservation medium comprises gentamicin at a concentration of at least 30 pg / mL. In some embodiments, the cryopreservation medium comprises gentamicin at a concentration of at least 20 pg / mL.

[0826] Any suitable closable vessel known to those skilled in the art in view of the present disclosure can be used in die methods described herein. Examples of suitable closable vessels include, but are not limited to, capped microcentrifuge tubes, lidded microcentrifuge tubes, and cryogenic specimen storage vials, including, but not limited to, cryovials. The term “cryogenic specimen storage vial” is meant to include the terms cryovial, cryo-container, cryogenic tube, and the like, including any and all closed, sealed, or re-closable containers (e.g., with screw caps or frictionally sealing snap caps) in which the container can be safely and securely stored at cryogenic temperatures (meaning at - 80C or below, and optionally submerged in liquid nitrogen or suspended in the vapor phase above liquid nitrogen at a temperature of approximately -196C). Capped or lidded microcentrifuge tubes and cryovials commonly fabricated from polyethylene or polypropylene are often used as cryogenic specimen storage vials.

[0827] In some embodiments, the closable vessel is filled from about 50% to about 85% volume with cryopreservation medium. In some embodiments, the closable vessel is filled from about 50% to about 85% volume with cryopreservation medium. In some embodiments, the closable vessel is filled from about 50% to about 75% volume with cry opreservation medium. In some embodiments, the closable vessel is filled from about 50% to about 65% volume with cryopreservation medium. In some embodiments, the closable vessel is filled from about 50% to about 55% volume with cryopreservation medium. In some embodiments, the closable vessel is filled from about 60% to about 85% volume with cryopreservation medium. In some embodiments, the closable vessel is filled from about 60% to about 75% volume with cryopreservation medium. In some embodiments, the closable vessel is filled from about 60% to about 65% volume with cryopreservation medium. In some embodiments, the closable vessel is filled from about 70% to about 85% volume with cryopreservation medium. In some embodiments, the closable vessel is filled from about 70% to about 75% volume with cryoprcscrvation medium. In some embodiments, the closable vessel is filled from about 80% to about 85% volume with cryopreservation medium.

[0828] In some embodiments, the pre-cooling step comprises placing the closable vessel in a controlled-rate freezing device that is at a temperature of about -80C to about 8C for a period of at least about 5 minutes to about 8 hours. In some embodiments, the pre-cooling step comprises placing the closable vessel in a controlled-rate freezing device that is at a temperature of about -80C, about - 79C, about -78C, about -77C, about -76C, about -75C, about -70C, about -65C, about -60C, about - 55C, about -50C, about -45C, about -40C, about -35C, about -30C, about -25C, about -20C, about - 15C, about -IOC, about -5C, about OC, about 1C, about 2C, about 3C, about 4C, about 5C, about 6C,about 7C, about 8C, or any temperature in between, for a period of at least about 5 minutes, at least about 10 minutes, at least about 15 minutes, at least about 20 minutes, at least about 25 minutes, at least about 30 minutes, at least about 35 minutes, at least about 40 minutes, at least about 45 minutes, at least about 50 minutes, at least about 55 minutes, at least about 1 hour, at least about 1.5 hours, at least about 2 hours, at least about 3 hours, al least about 4 hours, at least about 5 hours, at least about 6 hours, at least about 7 hours, at least about 8 hours, or more.

[0829] In some embodiments, closed vessels comprising tumor fragments and cry opreservation medium are incubated at a temperature of about 2-8C for a period of about 30 to 60 minutes before slow-freezing the vessels in the controlled-rate freezing device. In some embodiments, vessels comprising tumor fragments and cryopreservation medium are incubated at a temperature of about 2C, about 3C, about 4C, about 5C, about 6C, about 7C, about 8C, or any temperature in between, for a period of about 5 minutes, about 10 minutes, about 15 minutes, about 20 minutes, about 25 minutes, about 30 minutes, about 35 minutes, about 40 minutes, about 45 minutes, about 50 minutes, about 55 minutes, about 60 minutes, or more, before slow-freezing the vessels in the controlled-rate freezing device.

[0830] Any suitable controlled-rate freezing device known to those skilled in the art in view of the present disclosure can be used in the methods described herein. Examples of suitable controlled-rate freezing devices include, but are not limited to, a Coming CoolCell™ device or a Nalgene Mr Frosty™ device. In some embodiments, the controlled-rate freezing device is an 1PA- free controlled rate freezing device that cools at a rate of about -0. IC / min to about -lOC / min. In some embodiments, the controlled-rate freezing device is an IPA-free controlled rate freezing device that cools at a rate of about -0. IC / min to about -lOC / min, about -0.2C / min to about -5C / min, about - 0.5C / min to about -2.5C / min, about -IC / min to about -2C / min. In some embodiments, the controlled- rate freezing device is an IPA-free controlled rate freezing device that cools at a rate of about -1° C / min.

[0831] In some embodiments, all of the positions of the controlled-rate freezing device are filled with closable vessels containing cryopreservation medium. In some embodiments, 90% or more of the positions of the controlled-rate freezing device are filled with closable vessels containing cryopreservation medium. In some embodiments, 80% or more of the positions of the controlled-rate freezing device are filled with closable vessels containing cry opreservation medium. In some embodiments, 70% or more of the positions of the controlled-rate freezing device are filled with closable vessels containing cry opreservation medium. In some embodiments, 60% or more of the positions of the controlled-rate freezing device are filled with closable vessels containing cryopreservation medium. In some embodiments, 50% or more of the positions of the controlled-rate freezing device are filled with closable vessels containing cry opreservation medium. In someembodiments, 40% or more of the positions of the controlled-rate freezing device are filled with closable vessels containing cry opreservation medium.

[0832] The term, “slow freezing method” as used herein refers to a process in which a sample is cooled at a controlled rate in a cooling environment before final cryopreservation in liquid nitrogen or the like. In some embodiments, the cooling rate is about -O.lC / min to about -lOC / min, about -0.2C / min to about -5C / min, about -0.5C / min to about -2.5C / min, about -IC / min to about - 2C / min. In some embodiments, the cooling rate is about -IC / min. In some embodiments, the cooling environment is a -80C freezer set between about -90C and about -70C, such as about -90C, about - 89C, about -88C, about -87C, about -86C, about -85C, about -84C, about -83C, about -82C, about - 81C, about -80C, about -79C, about -78C, about -77C, about -76C, about -75C, about -74C, about - 73C, about -72C, about -72C, about -71C, or any temperature between, or dry ice.

[0833] In some embodiments, the slow-freezing comprises incubating the controlled-rate freezing device at a temperature of about -70°C to about -90°C. In some embodiments, the slow- freezing comprises incubating the controlled-rate freezing device at a temperature of about -75°C to about -85°C. In some embodiments, the slow-freezing comprises incubating the controlled-rate freezing device at a temperature of about -78°C to about -80°C. In some embodiments, the slow- freezing comprises incubating the controlled-rate freezing device with dry ice. In some embodiments, the slow-freezing comprises incubating the controlled-rate freezing device in a -80°C freezer. In some embodiments, the slow-freezing comprises incubating the controlled-rate freezing device in dry ice.

[0834] In some embodiments, the slow-freezing comprises incubating the controlled-rate freezing device at a temperature of about -80°C, for about 3-5 hours. In some embodiments, the slow- freezing comprises incubating the controlled-rate freezing device at a temperature of about -80°C, for about 3 hours. In some embodiments, the slow-freezing comprises incubating the controlled-rate freezing device at a temperature of about -80°C, for about 4 horns. In some embodiments, the slow- freezing comprises incubating the controlled-rate freezing device at a temperature of about -80°C, for about 5 hours.

[0835] In some embodiments, after recovery from freezing, the cells have a post-thaw viability of at least about 80%. In some embodiments, after recovery from freezing, the cells have a post-thaw viability of at least about 75%. In some embodiments, after recovery from freezing, the cells have a post-thaw viability of at least about 70%. In some embodiments, after recovery from freezing, the cells have a post-thaw viability of at least about 65%. In some embodiments, after recovery from freezing, the cells have a post-thaw viability of at least about 60%. In some embodiments, after recovery from freezing, the cells have a post-thaw viability of at least about 55%. In some embodiments, after recovery from freezing, the cells have a post-thaw viability of at leastabout 50%. In some embodiments, after recovery from freezing, the cells have a post-thaw viability of at least about 45%. In some embodiments, after recovery from freezing, the cells have a post-thaw viability of at least about 40%. In some embodiments, after recovery from freezing, the cells have a post-thaw viability of at least about 35%. In some embodiments, after recovery from freezing, the cells have a post-thaw viability of at least about 30%. In some embodiments, after recovery from freezing, the cells have a post-thaw viability of at least about 25%. In some embodiments, after recovery from freezing, the cells have a post-thaw viability of at least about 20%. Any suitable methods to measure or determine post-thaw viability known in the art in view of the present disclosure can be used in the methods described herein.

[0836] In some embodiments, tumor digests are generated by incubating the tumor in enzyme media, for example but not limited to RPMI 1640, 2mM GlutaMAX, 10 mg / mL gentamicin, 30 U / mL DNase, and 1.0 mg / mL collagenase, followed by mechanical dissociation (GentleMACS, Miltenyi Biotec, Auburn, CA). In some embodiments, the tumor is placed in a tumor dissociating enzyme mixture including one or more dissociating (digesting) enzymes such as, but not limited to, collagenase (including any blend or type of collagenase), Accutase™, Accumax™, hyaluronidase, neutral protease (dispase), chymotrypsin, chymopapain, try psin, caseinase, elastase, papain, protease type XIV (pronase), deoxyribonuclease I (DNase), trypsin inhibitor, any other dissociating or proteolytic enzyme, and any combination thereof. In other embodiments, the tumor is placed in a tumor dissociating enzyme mixture including collagenase (including any blend or type of collagenase), neutral protease (dispase) and deoxyribonuclease I (DNase).IV. Gene-Editing ProcessesA. Overview: TIL Expansion + Gene-Editing

[0001] In some embodiments of the present invention is directed to methods for expanding TIL populations, the methods comprise one or more steps of gene-editing at least a portion of the TILs in order to enhance their therapeutic effect. As used herein, “gene-editing,” “gene editing,” and “genome editing” refer to a type of genetic modification in which DNA is permanently modified in the genome of a cell, e g., DNA is inserted, deleted, modified or replaced within the cell’s genome. In some embodiments, gene-editing causes the expression of a DNA sequence to be silenced (sometimes referred to as a gene knockout) or inhibited / reduced (sometimes referred to as a gene knockdown). In other embodiments, gene-editing causes the expression of a DNA sequence to be enhanced (e.g., by causing over-expression). In accordance with embodiments of the present invention, gene-editing technology is used to enhance the effectiveness of a therapeutic population of TILs. Exemplary gene-editing processes / methods of the present invention, as well as gene-edited TIL products can also be found in International Patent Application No. PCT / US22 / 14425, U.S. Provisional Application Nos.63 / 304,498 and 63 / 242,373, all of which are incorporated herein by reference in their entireties for all related purposes.

[0002] A method for expanding tumor infiltrating lymphocytes (TILs) into a therapeutic population of TILs may be carried out in accordance with any embodiment of the methods described herein (e.g., an exemplary TIL expansion method known as process 2A is described below), wherein the method further comprises gene-editing at least a portion of the TILs. According to additional embodiments, a method for expanding TILs into a therapeutic population of TILs is carried out in accordance with any embodiment of the methods described in U.S. Pat. No. 10,517,894, U.S. Patent Application Publication No. 2020 / 0121719 Al, or U.S. Pat. No. 10,894,063, which are incorporated by reference herein in their entireties, wherein the method further comprises gene-editing at least a portion of the TILs. Thus, some embodiments of the present invention provides a therapeutic population of TILs that has been expanded in accordance with any embodiment described herein, wherein at least a portion of the therapeutic population has been gene-edited, e.g., at least a portion of the therapeutic population of TILs that is transferred to the infusion bag is permanently gene-edited.

[0003] In some embodiments of the present invention directed to methods for expanding TIL populations, the methods comprise one or more steps of introducing into at least a portion of the TILs nucleic acids, e.g., mRNAs, for transient expression of an immunomodulatory protein, e.g., an immunomodulatory fusion protein comprising an immunomodulatory' protein fused to a membrane anchor, in order to produce modified TILs with (i) reduced dependence on cytokines in when expanded in culture and / or (ii) an enhanced therapeutic effect. As used herein, “transient geneediting”, “transient gene editing”, “transient phenotypic alteration,” “transient phenotypic modification”, “temporary phenotypic alteration,” “temporary phenotypic modification”, “transient cellular change”, “transient cellular modification”, “temporary cellular alteration”, “temporary cellular modification”, “transient expression”, “transient alteration of expression”, “transient alteration of protein expression”, “transient modification”, “transitory phenotypic alteration”, “non-permanent phenotypic alteration”, “transiently modified”, “temporarily modified”, “non-permanently modified”, “transiently altered”, “temporarily altered”, grammatical variations of any of the foregoing, and any expressions of similar meaning, refer to a type of cellular modification or phenotypic change in which nucleic acid (e g., mRNA) is introduced into a cell, such as transfer of nucleic acid into a cell by electroporation, calcium phosphate transfection, viral transduction, etc., and expressed in the cell (e.g., expression of an immunomodulatory protein, such as an immunomodulatory fusion protein comprising an immunomodulatory protein fused to a membrane anchor) in order to effect a transient or non-permanent phenotypic change in the cell, such as the transient display of membrane-anchored immunomodulatory fusion protein on the cell surface. In accordance with embodiments of the present invention, transient phenotypic alteration technology is used to reduce dependence oncytokines in the expansion of TILs in culture and / or enhance the effectiveness of a therapeutic population of TILs.

[0004] In some embodiments, a microfluidic platform is used for intracellular delivery of nucleic acids encoding the immunomodulatory fusion proteins provided herein. In some embodiments, the microfluidic platform is a SQZ vector-free microfluidic platform. The SQZ platform is capable of delivering nucleic acids and proteins, to a variety of primary human cells, including T cells (Sharei et al. PNAS 2013, as well as Sharei ct al. PLOS ONE 2015 and Grcisbcck ct al. J. Immunology vol. 195, 2015). In the SQZ platform, the cell membranes of the cells for modification (e.g., TILs) are temporarily disrupted by microfluidic constriction, thereby allowing the delivery of nucleic acids encoding the immunomodulatory fusion proteins into the cells. Such methods as described in International Patent Application Publication Nos. WO 2013 / 059343A1, WO 2017 / 008063A1, or WO 2017 / 123663A1, or U.S. Patent Application Publication Nos. US 2014 / 0287509A1, US 2018 / 0201889A1, or US 2018 / 0245089A1 (incorporated herein by reference in their entirties) can be employed with the present invention for delivering nucleic acids encoding the subject immunomodulatory fusion proteins to a population of TILs. In some embodiments, the delivered nucleic acid allows for transient protein expression of the immunomodulatory fusion proteins in the modified TILs. In some embodiments, the SQZ platform is used for stable incorporation of the delivered nucleic acid encoding the immunomodulatory fusion protein into the TIL cell genome. Additional exemplary disclosures for the SQZ platform and its use can be found in International Patent Application Publication No. WO / 2019 / 136456, which is incorporated herein by reference in its entirety for all purposes.B. Timing of Gene-Editing / Transient Phenotypic Alteration During TIL Expansion

[0005] According to some embodiments, a method for expanding tumor infiltrating lymphocytes (TILs) into a therapeutic population of TILs comprises:(a) obtaining a first population of TILs from a tumor resected from a patient by processing a tumor sample obtained from the patient into multiple tumor fragments;(b) adding the tumor fragments into a closed system;(c) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2, and optionally OKT-3 (e.g., OKT-3 may be present in the culture medium beginning on the start date of the expansion process), to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-14 days to obtain the second population of TILs, and wherein the transition from step (b) to step (c) occurs without opening the system;(d) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, optionally OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-14 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the second expansion is performed in a closed container providing a second gas- permeable surface area, and wherein the transition from step (c) to step (d) occurs without opening the system;(e) harvesting the therapeutic population of TILs obtained from step (d), wherein the transition from step (d) to step (e) occurs without opening the system;(f) transferring the harvested TIL population from step (e) to an infusion bag, wherein the transfer from step (e) to (f) occurs without opening the system; and(g) at any time during the method prior to the transfer to the infusion bag in step (1), geneediting at least a portion of the TIL cells to express an immunomodulatory composition comprising an immunomodulatory agent (e.g., a membrane anchored immunomodulatory fusion protein described herein) on the surface of the TIL cells. In some embodiments, the immunomodulatory agent is selected from the group consisting of IL-2, IL-7, IL-10, IL-12, IL-15, IL-18, IL-21 and a CD40 agonist (e.g., CD40L or an agonistic CD40 binding domain). In some embodiments, the immunomodulatory agent is selected from the group consisting of IL-2, IL-12, IL-15, IL-18, IL-21 and a CD40 agonist. In some embodiments, the immunomodulatory agent is selected from the group consisting of IL-12, IL-15, IL-18, IL-21, and a CD40 agonist.

[0006] As stated in step (g) of the embodiments described above, the gene-editing process may be carried out at any time during the TIL expansion method prior to the transfer to the infusion bag in step (f), which means that the gene editing may be carried out on TILs before, during, or after any of the steps in the expansion method; for example, during any of steps (a)-(f) outlined in the method above, or before or after any of steps (a)-(e) outlined in the method above. According to certain embodiments, TILs are collected during the expansion method (e.g., the expansion method is “paused” for at least a portion of the TILs), and the collected TILs are subjected to a gene-editing process, and, in some cases, subsequently reintroduced back into the expansion method (e.g. , back into the culture medium) to continue the expansion process, so that at least a portion of the therapeutic population of TILs that are eventually transferred to the infusion bag are permanently gene-edited. In some embodiments, the gene-editing process may be carried out before expansion by activating TILs, performing a gene-editing step on the activated TILs, and expanding the gene-edited TILs according to the processes described herein. In some embodiments, nucleic acids for gene editing are deliveredto the TILs using a microfluidic platform. In some embodiments, the microfluidic platform is a SQZ vector -free microfluidic platform.

[0007] In some embodiments, the gene-editing process is carried out after the first TIL expansion step. In some embodiments, the gene-editing process is carried out after the first TIL expansion step and before the second expansion step. In some embodiments, the gene-editing process is carried out after the TILs are activated. In some embodiments, the gene-editing process is carried out after the first expansion step and after the TILs arc activated, but before the second expansion step. In some embodiments, the gene-editing process is carried out after the first expansion step and after the TILs are activated, and the TILs are rested after gene-editing and before the second expansion step. In some embodiments, the TILs are rested for about 1 to 2 days after gene-editing and before the second expansion step. In some embodiments, the TILs are activated by exposure to an anti-CD3 agonist and an anti-CD28 agonist. In some embodiments, the anti-CD3 agonist is an anti-CD3 agonist antibody and the anti-CD28 agonist is an anti-CD28 agonist antibody. In some embodiments, the anti-CD3 agonist antibody is OKT-3. In some embodiments, the TILs are activated by exposure to anti-CD3 agonist antibody- and anti-CD28 agonist antibody -conjugated beads. In some embodiments, the anti- CD3 agonist antibody- and anti-CD28 agonist antibody -conjugated beads are the TransAct™ product of Miltenyi. In some embodiments, the gene-editing process is carried out by viral transduction. In some embodiments, the gene-editing process is carried out by retroviral transduction. In some embodiments, the gene-editing process is carried out by lentiviral transduction. In some embodiments, the immunomodulatory composition is a membrane anchored immunomodulatory fusion protein. In some embodiments, the immunomodulatory fusion protein comprises IL-15. In some embodiments, the immunomodulatory fusion protein comprises IL-21. In some embodiments, the immunomodulatory composition comprises two or more different membrane bound fusion proteins. In some embodiments, the immunomodulatory composition comprises a first immunomodulatory protein comprising IL- 15 and a second immunomodulatory fusion protein comprising IL-21. In some embodiments, the TILs are gene-edited to express the immunomodulatory composition under the control of an NF AT promoter. In some embodiments, the TILs are gene-edited to express an immunomodulatory fusion protein comprising IL- 15 under the control of an NF AT promoter. In some embodiments, the TILs are gene-edited to express an immunomodulatory fusion protein comprising IL-21 under the control of an NF AT promoter. In some embodiments, the TILs are gene-edited to express a first immunomodulatory fusion protein comprising IL- 15 and a second immunomodulatory fusion protein comprising IL-21 under the control of an NF AT promoter.

[0008] In some embodiments, the gene-editing process is carried out by viral transduction. In some embodiments, the gene-editing process is carried out by retroviral transduction. In some embodiments, the gene-editing process is carried out by lentiviral transduction.

[0009] According to some embodiments, a method for expanding tumor infiltrating lymphocytes (TILs) into a therapeutic population of TILs comprises:(a) obtaining a first population of TILs from a tumor resected from a patient by processing a tumor sample obtained from the patient into multiple tumor fragments;(b) adding the tumor fragments into a closed system;(c) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2, and optionally OKT-3 (e.g., OKT-3 may be present in the culture medium beginning on the start date of the expansion process), to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-14 days to obtain the second population of TILs, and wherein the transition from step (b) to step (c) occurs without opening the system;(d) gene-editing at least a portion of the TIL cells in the second population of TILs to express an immunomodulatory composition comprising an immunomodulatory agent (e.g., a membrane anchored immunomodulatory fusion protein described herein) on the surface of the TIL cells;(e) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, optionally OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-14 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the second expansion is performed in a closed container providing a second gas- permeable surface area, and wherein the transition from step (c) to step (d) occurs without opening the system;(f) harvesting the therapeutic population of TILs obtained from step (d), wherein the transition from step (d) to step (e) occurs without opening the system; and

[0010] (g) transferring the harvested TIL population from step (e) to an infusion bag, wherein the transfer from step (e) to (f) occurs without opening the system. In some embodiments, the immunomodulatory agent is selected from the group consisting of IL-2, IL-7, IL-10, IL-12, IL-15, IL- 18, IL-21 and a CD40 agonist (e.g., CD40L or an agonistic CD40 binding domain). In some embodiments, the immunomodulatory agent is selected from the group consisting of IL-2, IL-12, IL- 15, IL- 18, IL-21 and a CD40 agonist. In some embodiments, the immunomodulatory agent is selected from the group consisting of IL-12, IL-15, IL-18, IL-21, and a CD40 agonist. In some embodiments, the TILs are rested after the gene-editing step and before the second expansion step. In some embodiments, the TILs are rested for about 1 to 2 days after the gene-editing step and before the second expansion step. In some embodiments, the TILs are activated by exposure to an anti-CD3agonist and an anti-CD28 agonist. In some embodiments, the anti-CD3 agonist is an anti-CD3 agonist antibody and the anti-CD28 agonist is an anti-CD28 agonist antibody. In some embodiments, the anti-CD3 agonist antibody is OKT-3. In some embodiments, the TILs are activated by exposure to anti-CD3 agonist antibody- and anti-CD28 agonist antibody -conjugated beads. In some embodiments, the anti-CD3 agonist antibody - and anti-CD28 agonist antibody -conjugated beads are the TransAct™ product of Miltenyi. In some embodiments, the gene-editing process is carried out by viral transduction. In some embodiments, the gene-editing process is carried out by retroviral transduction of the TILs, optionally for about 2 days. In some embodiments, the gene-editing process is carried out by lentiviral transduction of the TILs, optionally for about 2 days. In some embodiments, the immunomodulatory composition is a membrane anchored immunomodulatory fusion protein. In some embodiments, the immunomodulatory fusion protein comprises IL- 15. In some embodiments, the immunomodulatory fusion protein comprises IL-21. In some embodiments, the immunomodulatory composition comprises two or more different membrane bound fusion proteins. In some embodiments, the immunomodulatory composition comprises a first immunomodulatory protein comprising IL- 15 and a second immunomodulatory fusion protein comprising IL-21. In some embodiments, the TILs are gene-edited to express the immunomodulatory composition under the control of an NF AT promoter. In some embodiments, the TILs are gene-edited to express an immunomodulatory fusion protein comprising IL- 15 under the control of an NF AT promoter. In some embodiments, the TILs are gene-edited to express an immunomodulatory fusion protein comprising IL-21 under the control of an NF AT promoter. In some embodiments, the TILs are gene-edited to express a first immunomodulatory fusion protein comprising IL- 15 and a second immunomodulatory fusion protein comprising IL-21 under the control of an NF AT promoter.

[0011] It should be noted that alternative embodiments of the expansion process may differ from the method shown above; e.g., alternative embodiments may not have the same steps (a)-(g), or may have a different number of steps. Regardless of the specific embodiment, the gene-editing process may be carried out at any time during the TIL expansion method. For example, alternative embodiments may include more than two expansions, and it is possible that gene-editing may be conducted on the TILs during a third or fourth expansion, etc.

[0012] According to other embodiments, a method for expanding tumor infiltrating lymphocytes (TILs) into a therapeutic population of TILs comprises:(a) obtaining a first population of TILs from a tumor resected from a patient by processing a tumor sample obtained from the patient into multiple tumor fragments;(b) adding the tumor fragments into a closed system;(c) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2, and optionally OKT-3 (e.g., OKT-3 may be present in the culture medium beginning on the start date of the expansion process), to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-14 days to obtain the second population of TILs, and wherein the transition from step (b) to step (c) occurs without opening the system;(d) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, optionally OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansi...

Claims

WHAT IS CLAIMED IS:

1. A method of making a cryopreserved population of TILs from a NSCLC patient, wherein the patient is naive to a cancer treatment, the method comprising:(a) prior to the patient receives a cancer treatment, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cryopreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest; wherein the patient subsequently receives an ICI treatment and / or a standard of care treatment; optionally wherein the first population of TILs is further expanded into a therapeutic population of TILs if the patient exhibits progressive disease on or after the ICI and / or standard of care treatment.

2. A method of making a cryopreserved population of TILs from a NSCLC patient, wherein the patient is on a maintaience therapy that is interrupted, the method comprising:(a) prior to the resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cryopreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest; wherein the patient subsequently resumes the maitnainence therapy; optionally wherein the first population of TILs is further expanded into a therapeutic population of TILs if the patient exhibits progressive disease on or after the resumption of the maintenance therapy.

3. A method of making a cryopreserved population of TILs from a N SCLC patient, wherein the patient is naive to a cancer treatment, comprising:(a) prior to the patient receives a cancer treatment, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cry opreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) thawing the cryopreserved tumor fragments or tumor digest and adding the firstpopulation of TILs into a closed system;(d) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-14 days to obtain the second population of TILs, and wherein the transition from step (c) to step (d) occurs without opening the system;(e) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-14 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the second expansion is performed in a closed container providing a second gas-permeable surface area, and wherein the transition from step (d) to step (e) occurs without opening the system;(f) harvesting therapeutic population of TILs obtained from step (e), wherein the transition from step (e) to step (I) occurs without opening the system; and(g) transferring the harvested TIL population from step (f) to an infusion bag, wherein the transfer from step (f) to (g) occurs without opening the system; and(h) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cryopreservation process. A method of making a cryopreserved population of TILs from a NSCLC patient, wherein the patient is naive to a cancer treatment, comprising:(a) prior to the patient receives a cancer treatment, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cry opreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) thawing the cryopreserved tumor fragments or tumor digest and adding the first population of TILs into a closed system;(d) performing a priming first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2, OKT-3, and antigen presenting cells (APCs) to produce a second population of TILs, wherein the priming first expansion is performed in a container comprising a first gas-permeable surface area, wherein the priming first expansion is performed for first period of about 1 to 7 / 8 days to obtain the second population of TILs, wherein the second population of TILs is greater in number than thefirst population of TILs;(e) performing a rapid second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and APCs, to produce a third population of TILs, wherein the number of APCs added in the rapid second expansion is at least twice the number of APCs added in step (b), wherein the rapid second expansion is performed for a second period of about 1 to 11 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the rapid second expansion is performed in a container comprising a second gas-permeable surface area;(f) harvesting therapeutic population of TILs obtained from step (e), wherein the transition from step (e) to step (I) occurs without opening the system; and(g) transferring the harvested TIL population from step (I) to an infusion bag, wherein the transfer from step (I) to (g) occurs without opening the system; and(h) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cryopreservation process. The method of claim 4, wherein the rapid expansion can proceed for 1 day, 2 days, 3 days, 4, days, 5 days, 6 days, 7 days, 8 days, 9 days or 10 days after initiation of the rapid expansion. A method of making a cryopreserved population of TILs from a NSCLC patient, wherein the patient is on maintenance therapy that is interrupted, comprising:(a) prior to the resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cry opreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) thawing the cryopreserved tumor fragments or tumor digest and adding the first population of TILs into a closed system;(d) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-14 days to obtain the second population of TILs, and wherein the transition from step (c) to step (d) occurs without opening the system;(e) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and antigen presenting cells(APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-14 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the second expansion is performed in a closed container providing a second gas-permeable surface area, and wherein the transition from step (d) to step (e) occurs without opening the system;(f) harvesting therapeutic population of TILs obtained from step (e), wherein the transition from step (e) to step (f) occurs without opening the system; and(g) transferring the harvested TIL population from step (f) to an infusion bag, wherein the transfer from step (f) to (g) occurs without opening the system; and(h) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process. A method of making a cryopreserved population of TILs from a NSCLC patient, wherein the patient is on maintenance therapy that is interrupted, comprising:(a) prior to the resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cry opreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) thawing the cryopreserved tumor fragments or tumor digest and adding the first population of TILs into a closed system;(d) performing a priming first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2, OKT-3, and antigen presenting cells (APCs) to produce a second population of TILs, wherein the priming first expansion is performed in a container comprising a first gas-permeable surface area, wherein the priming first expansion is performed for first period of about 1 to 7 / 8 days to obtain the second population of TILs, wherein the second population of TILs is greater in number than the first population of TILs;(e) performing a rapid second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and APCs, to produce a third population of TILs, wherein the number of APCs added in the rapid second expansion is at least twice the number of APCs added in step (b), wherein the rapid second expansion is performed for a second period of about 1 to 11 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the rapid second expansion is performed in a container comprising a second gas-permeablesurface area;(f) harvesting therapeutic population of TILs obtained from step (e), wherein the transition from step (e) to step (f) occurs without opening the system; and(g) transferring the harvested TIL population from step (f) to an infusion bag, wherein the transfer from step (f) to (g) occurs without opening the system; and(h) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cryopreservation process. The method of any one of the preceding claims, wherein the cancer patient is naive to any cancer treatment. The method of any one of the preceding claims, wherein the patient is naive to ICI treatment, anti- VEGF treatment, or chemotherapeutic treatment. The method of any one of the preceding claims, wherein the patient is on maintenance therapy that is interrupted at the time of step (a), and the maintenance therapy is resinned after step (a). The method of claim 10, wherein the patient is in a washout period at initiation of step (a). The method of claim 10, wherein step of thawing and the subsequent steps are performed after the patient receives the cancer treatment or resumes the maintenance therapy and the patient exhibits progressive disease. The method of any one of the preceding claims, wherein the patient exhibits progressive disease at least about 1 week, 2 weeks, 3 weeks, 1 month, 2 months, 3 month, 4 months, 5 months, 6 months, 7 months, 8 months, 9 months, 10 months, 11 months, 12 months, 13 months, 14 months, 15 months, 16 months, 17 months, 18 months, 19 months, 20 months, 21 months, 22 months, 23 months, 24 months, 25 months, 26 months, 27 months, 28 months, 29 months, 30 months, 31 months, 32 months, 33 months, 34 months, 35 months, 36 months, after the step (b) of cry opreserving. The method of any one of the preceding claims, wherein the patient receives or is going to receive a first-line ICI treatment and / or standard treatment. The method of any one of the preceding claims, wherein the patient receives or is going to receive a second-line ICI treatment and / or standard treatment. The method of any one of the preceding claims, wherein the patient is diagnosed with NSCLC. The method of any one of the preceding claims, wherein the patient is diagnosed with metastatic stage IV NSCLC. The method of any one of the preceding claims, wherein the subject or patient has at least one of:i) a predetermined tumor proportion score (TPS) of PD-L1 of < 1%, ii) a TPS score of PD-L1 of l%-49%, or iii) a predetermined absence of one or more driver mutations. The method of any of the preceding claims, wherein the patient or subject has a TPS of PD-L1 of l%-49%. The method of any of the preceding claims, wherein the patient or subject has a TPS of PD-L1 of < 1% and has a predetermined absence of one or more driver mutations. The method of any of the preceding claims, wherein the one or more driver is selected from the group consisting of an EGFR mutation, an EGFR insertion, EGFR exon20, a KRAS mutation, a BRAF-mutation, a BRAF V600 mutation, an ALK-mutation, a c -ROS -mutation (ROS1- mutation), a ROS1 fusion, a RET mutation, a RET fusion, an ERBB2 mutation, an ERBB2 amplification, a BRCA mutation, a MAP2K1 mutation, PIK3CA, CDKN2A, a PTEN mutation, an UMD mutation, an NRAS mutation, a KRAS mutation, an NF1 mutation, a MET mutation, a MET splice and / or altered MET signaling, a TP53 mutation, a CREBBP mutation, a KMT2C mutation, a KMT2D mutation, an ARID 1 A mutation, a RBI mutation, an ATM mutation, a SETD2 mutation, a FLT3 mutation, a PTPN11 mutation, a FGFR1 mutation, an EP300 mutation, a MYC mutation, an EZH2 mutation, a JAK2 mutation, a FBXW7 mutation, a CCND3 mutation, and a GNA11 mutation. The method of any of the preceding claims, wherein the patient or subject has a TPS of < 1% and has a NSCLC that is not indicated for treatment by an EGFR inhibitor, a BRAF inhibitor, an ALK inhibitor, a c-Ros inhibitor, a RET inhibitor, an ERBB2 inhibitor, BRCA inhibitor, a MAP2K1 inhibitor, PIK3CA inhibitor, CDKN2A inhibitor, a PTEN inhibitor, an UMD inhibitor, an NRAS inhibitor, a KRAS inhibitor, an NF1 inhibitor, MET inhibitor a TP53 inhibitor, a CREBBP inhibitor, a KMT2C inhibitor, a KMT2D mutation, an ARID1A mutation, a RBI inhibitor, an ATM inhibitor, a SETD2 inhibitor, a FLT3 inhibitor, a PTPN 11 inhibitor, a FGFR1 inhibitor, an EP300 inhibitor, a MYC inhibitor, an EZH2 inhibitor, a JAK2 inhibitor, a FBXW7 inhibitor, a CCND3 inhibitor, and a GNA11 inhibitor. The method of claim 20, wherein the one or more driver mutations comprises a genomic alteration of EGFR, ALK, or ROS. The method of claim 20, wherein the one or more driver mutations consists of a genomic alteration of EGFR, ALK, or ROS. The method of any of the preceding claims, wherein the NSCLC has low or no expression of PD- Ll.

26. The method of any of the preceding claims, wherein the patient has a predetermined absence of one or more driver mutations.Tl. The method of any of the preceding claims, wherein the NSCLC is refractory or resistant to treatment with a chemotherapeutic agent.

28. The method of any of the preceding claims, wherein the NSCLC is refractory or resistant to treatment with a VEGF-A inhibitor.

29. The method of any of the preceding claims, wherein the NSCLC has been treated with a chemotherapeutic agent but is not being currently treated with a chemotherapeutic agent.

30. The method of any of the preceding claims, wherein the NSCLC has been treated with a chemotherapeutic agent but is not being currently treated with a chemotherapeutic agent and has a TPS of < 1%.

1. The method of any of the preceding claims, wherein the NSCLC has been treated with a VEGF-A inhibitor but is not being currently treated with a VEGF-A inhibitor.

32. The method of any of the preceding claims, wherein the NSCLC has been treated with a VEGF-A inhibitor but is not being currently treated with a VEGF-A inhibitor and has a TPS of < 1%.

33. The method of any of the preceding claims, wherein the NSCLC has been treated with a chemotherapeutic agent and / or a VEGF-A inhibitor, but is not being currently treated with a chemotherapeutic agent and / or a VEGF-A inhibitor.

34. The method of any of the preceding claims, wherein the NSCLC has been treated with a chemotherapeutic agent and / or a VEGF-A inhibitor but is not being currently treated with a chemotherapeutic agent and / or a VEGF-A inhibitor and has a TPS of < 1%.

35. The method of any of the preceding claims, wherein the NSCLC has not been previously treated with an anti-PD-1 and / or anti-PD-Ll antibody.

36. The method of any of the preceding claims, wherein the NSCLC has not been previously treated with an anti-PD-1 and / or anti-PD-Ll antibody and has been previously treated a chemotherapeutic agent and / or a VEGF-A inhibitor.

37. The method of any of the preceding claims, wherein the NSCLC has not been previously treated with an anti-PD-1 and / or anti-PD-Ll antibody and has been previously treated a chemotherapeutic agent and / or a VEGF-A inhibitor but is not being currently treated with a chemotherapeutic agent and / or a VEGF-A inhibitor.

38. The method of any of the preceding claims, wherein the NSCLC has not been previously treated with an anti-PD-1 and / or anti-PD-Ll antibody and has been previously treated a VEGF-Ainhibitor but is not being currently treated with a VEGF-A inhibitor. The method of any of the preceding claims, wherein the NSCLC has not been previously treated with an anti-PD-1 and / or anti-PD-Ll antibody and has been previously treated a chemotherapeutic agent and / or a VEGF-A inhibitor but is not being currently treated with a chemotherapeutic agent and / or a VEGF-A inhibitor. The method of any of the preceding claims, wherein the NSCLC has not been previously treated with an anti-PD-1 and / or anti-PD-Ll antibody and has low or no expression of PD-L1. The method of any of the preceding claims, wherein the NSCLC has not been previously treated with an anti-PD-1 and / or anti-PD-Ll antibody and has been previously treated a chemotherapeutic agent and / or a VEGF-A inhibitor but is not being currently treated with a chemotherapeutic agent and / or a VEGF-A inhibitor and has a TPS of < 1%. The method of any of the preceding claims, wherein the NSCLC has been previously treated with an anti-PD-1 and / or anti-PD-Ll and / or anti-PD-L2 antibody. The method of any of the preceding claims, wherein the NSCLC has been previously treated with an anti-PD-1 and / or anti-PD-Ll antibody and has been previously treated a chemotherapeutic agent and / or a VEGF-A inhibitor. The method of any of the preceding claims, wherein the NSCLC is refractory or resistant to treatment with an anti-PD-1 and / or anti-PD-Ll antibody. The method of any of the preceding claims, wherein the NSCLC has been previously treated with an anti-PD-1 and / or anti-PD-Ll antibody and the tumor proportion score was determined prior to the anti-PD-1 and / or anti-PD-Ll antibody treatment. The method of any of the preceding claims, wherein the NSCLC has been previously treated with an anti-PD-Ll antibody and tire tumor proportion score was determined prior to the anti-PD-Ll antibody treatment, or the NSCLC has been previously treated with an anti-PD-1 antibody and the tumor proportion score was determined prior to the anti-PD-1 antibody treatment. The method of any of the preceding claims, wherein the NSCLC has been treated with a chemotherapeutic agent and / or a VEGF-A inhibitor. The method of any of the preceding claims, wherein the NSCLC has not been previously treated with an anti-PD-1 and / or anti-PD-Ll antibody and has bulky disease at baseline. The method of any of the preceding claims, wherein the NSCLC has been previously treated with an anti-PD-1 and / or anti-PD-Ll antibody and has bulky disease at baseline. The method of any of the preceding claims, wherein the NSCLC has been treated with achemotherapeutic agent and has bulky disease at baseline. The method of any of the preceding claims, wherein the NSCLC has been treated with a chemotherapeutic agent and / or VEGF-A inhibitor but is not being currently treated with a chemotherapeutic agent and / or VEGF-A inhibitor and has bulky disease at baseline. The method of any of the preceding claims, wherein bulky disease is indicated where the maximal tumor diameter is greater than 7 cm measured in either the transverse or coronal plane or swollen lymph nodes with a short-axis diameter of 20 mm or greater. The method of any of the preceding claims, wherein the NSCLC is refractory or resistant to at least two prior systemic treatment courses, not including neo-adjuvant or adjuvant therapies. The method of any of the preceding claims, wherein the NSCLC is refractory or resistant to an anti-PD-1 or an anti-PD-Ll antibody selected from the group consisting of nivolumab, pembrolizumab, JS001, TSR-042, pidilizumab, BGB-A317, SHR-1210, REGN2810, MDX-1106, PDR001, anti-PD-1 from clone: RMP1-14, anti-PD-1 antibodies disclosed in U.S. Patent No. 8,008,449, durvalumab, atezolizumab, avelumab, and fragments, derivatives, variants, as well as biosimilars thereof. The method of any of the preceding claims, wherein the NSCLC is refractory or resistant to pembrolizumab or a biosimilar thereof. The method of any of the preceding claims, wherein the NSCLC is refractory or resistant to nivolumab or a biosimilar thereof. The method of any of the preceding claims, wherein the NSCLC is refractory or resistant to an anti-CTLA-4 antibody. The method of any of the preceding claims, wherein the NSCLC is refractory or resistant to an anti- CTLA -4 antibody and pembrolizumab or a biosimilar thereof. The method of any of the preceding claims, wherein the NSCLC is refractory or resistant to an anti- CTLA -4 antibody, and nivolumab or a biosimilar thereof. The method of any of the preceding claims, wherein the anti-CTLA-4 antibody is ipilimumab or a biosimilar thereof. The method of any of the preceding claims, wherein the NSCLC is refractory or resistant to durvalumab or a biosimilar thereof. The method of any of the preceding claims, wherein the NSCLC is refractory or resistant to atezolizumab or a biosimilar thereof. The method of any of the preceding claims, wherein the NSCLC is refractory or resistant toavelumab or a biosimilar thereof. The method of any of the preceding claims, wherein the chemotherapeutic agent is a platinum doublet chemotherapeutic agent(s). The method of Claim 64, wherein the platinum doublet chemotherapeutic agent therapy comprises: i) a first chemotherapeutic agent selected from the group consisting of cisplatin and carboplatin, ii) and a second chemotherapeutic agent selected from the group consisting of vinorelbine, gemcitabine and a taxane (including for example, paclitaxel, docetaxel or nab-paclitaxel). The method of any of the preceding claims, wherein the chemotherapeutic agent, including the first and / or second chemotherapeutic agent, is in combination with pemetrexed. The method of any of the preceding claims, wherein the NSCLC is refractory or resistant to a combination therapy comprising carboplatin, paclitaxel, pemetrexed, and cisplatin. The method of any of the preceding claims, wherein the NSCLC is refractory or resistant to a combination therapy comprising carboplatin, paclitaxel, pemetrexed, cisplatin, nivolumab, and ipilimumab. The method of any of the preceding claims, wherein the NSCLC is refractory or resistant to a VEGF-A inhibitor. The method of any of the preceding claims, wherein the NSCLC is refractory or resistant to a VEGF-A inhibitor selected from the group consisting of bevacizumab, ranibizumab, and icrucumab. The method of any of the preceding claims, wherein the NSCLC is refractory or resistant to bevacizumab. The method of any of the preceding claims, wherein the NSCLC has been analyzed for the absence or presence of one or more driver mutations. The method of any of the preceding claims, wherein one or more driver mutations arc not present. The method of any of the preceding claims, wherein the NSCLC treatment is independent of the presence or absence of one or more driver mutations. The method of any of the preceding claims, wherein the one or more driver mutations is selected from the group consisting of an EGFR mutation, an EGFR insertion, a KRAS mutation, a BRAF- mutation, an ALK-mutation, a c-ROS-mutation a c-ROS-mutation, EML4-ALK, and MET mutation.The method of Claim 75, wherein the EGFR mutation results in tumor transformation from NSCLC to small cell lung cancer (SCLC). The method of any of the preceding claims, wherein the NSCLC treatment is independent of the presence or absence of high -tumor mutational burden (high-TMB) and / or microsatellite instability-high (MSI-high) status. The method of any of the preceding claims, wherein the NSCLC exhibits high-TMB and / or MSI- high status. A method of making a cryopreserved population of TILs from a cancer patient, comprising:(a) prior to the patient receives a cancer treatment, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cry opreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) waiting for the patient to exhibit progressive disease on or after the cancer treatment;(d) thawing the cryopreserved tumor fragments or tumor digest and adding the first population of TILs into a closed system;(e) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-14 days to obtain the second population of TILs, and wherein the transition from step (c) to step (d) occurs without opening the system;(f) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-14 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the second expansion is performed in a closed container providing a second gas-permeable surface area, and wherein the transition from step (e) to step (I) occurs without opening the system;(g) harvesting therapeutic population of TILs obtained from step (I), wherein the transition from step (f) to step (g) occurs without opening the system; and(h) transferring the harvested TIL population from step (I) to an infusion bag, wherein the transfer from step (g) to (h) occurs without opening the system; and(i) cryopreserving the infusion bag comprising the harvested TIL population from step(g) using a cryopreservation process. A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is on maintenance therapy that is interrupted, comprising:(a) prior to the resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cry opreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) waiting for the patient to exhibit progressive disease on or after the cancer treatment;(d) thawing the cryopreserved tumor fragments or tumor digest and adding the first population of TILs into a closed system;(e) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-14 days to obtain the second population of TILs, and wherein the transition from step (c) to step (d) occurs without opening the system;(I) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-14 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the second expansion is performed in a closed container providing a second gas-permeable surface area, and wherein the transition from step (e) to step (f) occurs without opening the system;(g) harvesting therapeutic population of TILs obtained from step (f), wherein the transition from step (f) to step (g) occurs without opening the system; and(h) transferring the harvested TIL population from step (f) to an infusion bag, wherein the transfer from step (g) to (h) occurs without opening the system; and(i) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process. A method of making a cryopreserved population of TILs from a cancer patient, comprising:(a) prior to the patient receives a cancer treatment, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cry opreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) waiting for the patient to exhibit progressive disease on or after the cancer treatment;(d) thawing the cryopreserved tumor fragments or tumor digest and adding the first population of TILs into a closed system;(e) performing a priming first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2, OKT-3, and antigen presenting cells (APCs) to produce a second population of TILs, wherein the priming first expansion is performed in a container comprising a first gas-permeable surface area, wherein the priming first expansion is performed for first period of about 1 to 7 / 8 days to obtain the second population of TILs, wherein the second population of TILs is greater in number than the first population of TILs;(f) performing a rapid second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and APCs, to produce a third population of TILs, wherein the number of APCs added in the rapid second expansion is at least twice the number of APCs added in step (b), wherein the rapid second expansion is performed for a second period of about 1 to 11 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the rapid second expansion is performed in a container comprising a second gas-permeable surface area;(g) harvesting therapeutic population of TILs obtained from step (f), wherein the transition from step (f) to step (g) occurs without opening the system; and(h) transferring the harvested TIL population from step (f) to an infusion bag, wherein the transfer from step (g) to (h) occurs without opening the system; and(i) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process. The method of claim 81, wherein the rapid expansion can proceed for 1 day, 2 days, 3 days, 4, days, 5 days, 6 days, 7 days, 8 days, 9 days or 10 days after initiation of the rapid expansion. A method of making a cryopreserved population of TILs from a cancer patient, comprising:(a) prior to the patient receives a cancer treatment, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cry opreserving the tumor fragments or tumor digest comprising the first population ofTILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) waiting for the patient to exhibit progressive disease on or after the cancer treatment;(d) thawing the cryopreserved tumor fragments or tumor digest and adding the first population of TILs into a closed system;(e) performing a priming first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2, OKT-3, and antigen presenting cells (APCs) to produce a second population of TILs, wherein the priming first expansion is performed in a container comprising a first gas-permeable surface area, wherein the priming first expansion is performed for first period of about 1 to 7 / 8 days to obtain the second population of TILs, wherein the second population of TILs is greater in number than the first population of TILs;(f) performing a rapid second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and APCs, to produce a third population of TILs, wherein the number of APCs added in the rapid second expansion is at least twice the number of APCs added in step (b), wherein the rapid second expansion is performed for a second period of about 1 to 11 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the rapid second expansion is performed in a container comprising a second gas-permeable surface area;(g) harvesting therapeutic population of TILs obtained from step (f), wherein the transition from step (f) to step (g) occurs without opening the system; and(h) transferring the harvested TIL population from step (f) to an infusion bag, wherein the transfer from step (g) to (h) occurs without opening the system; and(i) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process. The method of any of the preceding claims, wherein the patient is naive to any cancer treatment. The method of any of the preceding claims, wherein the patient receives a first-line ICI treatment and / or standard treatment. The method of any of the preceding claims, wherein the patient receives a second-line ICI treatment and / or standard treatment. The method of any of the preceding claims, wherein step (b) comprises flash freezing of the tumor fragments or tumor digest. The method of any of the preceding claims, wherein the flash freezing comprises:i) incubating the tumor fragments or tumor digest in a cryopreservation medium; optionally incubating for about 30 minutes to about 60 minutes at about 2°C to about 8°C in a cry opreservation medium comprising 10% v / v DMSO, and ii) freezing the tumor wherein the freezing is flash freezing using the vapor phase of liquid nitrogen. The method of any of the preceding claims, wherein step (b) comprises controlled-rate freezing of the tumor fragments or tumor digest. The method of any of the preceding claims, wherein the controlled-rate freezing comprises: i) adding cry opreservation medium to a closable vessel; ii) pre-cooling the closable vessel in a controlled-rate freezing device; iii) placing the tumor in the closable vessel comprising cry opreservation medium and closing the vessel; iv) incubating the closed vessel comprising the tumor and cryopreservation medium at a temperature of about 2-8C for a time period of about 30 to 60 minutes; and v) slow-freezing the vessel in a controlled-rate freezing device. The method of any of the preceding claims, wherein the cancer patient is naive to any cancer treatment. The method of any of the preceding claims, wherein the patient is naive to ICI treatment, anti- VEGF treatment, or chemotherapeutic treatment. The method of any of the preceding claims, wherein the patient is on maintenance therapy that is interrupted at the time of step (a), and the maintenance therapy is resumed after step (a). The method of any of the preceding claims, wherein the patient receives a first-line ICI treatment and / or standard treatment. The method of any of the preceding claims, wherein the patient receives a second-line ICI treatment and / or standard treatment. The method of any of the preceding claims, wherein the patient is diagnosed with NSCLC. The method of any of the preceding claims, wherein the patient is diagnosed with metastatic stage IV NSCLC. A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining a first population of TILs from a tumor resected from a subject by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cryopreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) thawing the cryopreserved tumor fragments or tumor digest and adding the the first population of TILs into a closed system;(d) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-11 days to obtain the second population of TILs, and wherein the transition from step (c) to step (d) occurs without opening the system;(e) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-11 days to obtain the third population of TILs, wherein the second expansion is performed in a closed container providing a second gas-permeable surface area, and wherein the transition from step (d) to step (e) occurs without opening the system;(f) harvesting the third population of TILs obtained from step (e), wherein the transition from step (e) to step (f) occurs without opening the system;(g) transferring the harvested third TIL population from step (f) to an infusion bag, wherein the transfer from step (I) to (g) occurs without opening the system;(h) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process; A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cryoprcscrving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) thawing the cryopreserved tumor fragments or tumor digest and adding the first population of TILs into a closed system;(d) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-14 days to obtain the second population of TILs, and wherein the transition from step (c) to step (d) occurs without opening the system;(e) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-14 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the second expansion is performed in a closed container providing a second gas-permeable surface area, and wherein the transition from step (d) to step (e) occurs without opening the system;(f) harvesting therapeutic population of TILs obtained from step (e), wherein the transition from step (e) to step (I) occurs without opening the system; and(g) transferring the harvested TIL population from step (I) to an infusion bag, wherein the transfer from step (f) to (g) occurs without opening the system; and(h) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process. . A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining a first population of TILs from a tumor resected from a subject by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cryopreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) thawing the cryopreserved tumor fragments or tumor digest and adding the the first population of TILs into a closed system;(d) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-11 days to obtain the second population of TILs, and wherein the transition from step (c) to step (d) occurs without opening the system;(e) performing a second expansion by supplementing the cell culture medium of the secondpopulation of TILs with additional IL-2. OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-11 days to obtain the third population of TILs, wherein the second expansion is performed in a closed container providing a second gas-permeable surface area, and wherein the transition from step (d) to step (e) occurs without opening the s stem;(f) harvesting the third population of TILs obtained from step (e), wherein the transition from step (e) to step (f) occurs without opening the system;(g) transferring the harvested third TIL population from step (f) to an infusion bag, wherein the transfer from step (!) to (g) occurs without opening the system; and(h) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process. . A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from surgical resection, needle biopsy, core biopsy, small biopsy, or other means for obtaining a sample that contains a mixture of tumor and TIL cells from a tumor in the subject or patient,(b) cry opreserving the sample comprising the first population of TILs from step (a) to produce cryopreserved sample;(c) thawing the cryopreserved sample and adding the first population of TILs into a closed system;(d) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-11 days to obtain tire second population of TILs, and wherein the transition from step (c) to step (d) occurs without opening the system;(e) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-11 days to obtain the third population of TILs, wherein the second expansion is performed in a closed container providing a second gas-pcrmcablc surface area, and wherein the transition from step (d) to step (e) occurs without opening the system;(f) harvesting the third population of TILs obtained from step (e), wherein the transition from step (e) to step (f) occurs without opening the system;(g) transferring the harvested third TIL population from step (f) to an infusion bag, wherein the transfer from step (f) to (g) occurs without opening the system; and(h) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process. . A method of making a cry opreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, resecting a tumor from the subject or patient, the tumor comprising a first population of TILs, optionally from surgical resection, needle biopsy, core biopsy, small biopsy, or other means for obtaining a sample that contains a mixture of tumor and TIL cells from a tumor;(b) cryopreserving the sample comprising the first population of TILs from step (a) to produce cryopreserved sample;(c) thawing the cryopreserved sample and adding the first population of TILs into a closed system;(d) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-11 days to obtain the second population of TILs and wherein the transition from step (c) to step (d) occurs without opening the system;(e) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for about 7-11 days to obtain the third population of TILs, wherein the second expansion is performed in a closed container providing a second gas-permeable surface area, and wherein the transition from step (d) to step (e) occurs without opening the system;(f) harvesting the third population of TILs obtained from step (e), wherein the transition from step (e) to step (f) occurs without opening the system;(g) transferring the harvested third TIL population from step (e) to an infusion bag, wherein the transfer from step (f) to (g) occurs without opening the system; and(h) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cryoprcscrvation process. . A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor resected from the subject or patient by processing a tumor sample obtained from the subject into multiple tumor fragments or a tumor digest;(b) cry opreserving the tumor fragments or tumor digest comprising the first population of TILs from step (a) to produce cryopreserved tumor fragments or tumor digest;(c) thawing the cryopreserved tumor fragments or tumor digest and adding the first population of TILs into a closed system;(d) performing a priming first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2, OKT-3, and antigen presenting cells (APCs) to produce a second population of TILs, wherein the priming first expansion is performed in a container comprising a first gas-permeable surface area, wherein the priming first expansion is performed for first period of about 1 to 7 / 8 days to obtain the second population of TILs, wherein the second population of TILs is greater in number than the first population of TILs;(e) performing a rapid second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and APCs, to produce a third population of TILs, wherein the number of APCs added in the rapid second expansion is at least twice the number of APCs added in step (b), wherein the rapid second expansion is performed for a second period of about 1 to 11 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the rapid second expansion is performed in a container comprising a second gas-permeable surface area;(f) harvesting therapeutic population of TILs obtained from step (e), wherein the transition from step (e) to step (f) occurs without opening the system; and(g) transferring the harvested TIL population from step (f) to an infusion bag, wherein the transfer from step (f) to (g) occurs without opening the system; and(h) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process. . A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from surgical resection, needle biopsy, core biopsy, smallbiopsy, or other means for obtaining a sample that contains a mixture of tumor and TIL cells from the subject or patient;(b) cryopreserving the sample comprising the first population of TILs from step (a) to produce cryopreserved sample;(c) thawing the cryopreserved sample and contacting the first population of TILs with a first cell culture medium in a closed system;(d) performing a priming first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2, OKT-3, and antigen presenting cells (APCs) to produce a second population of TILs, wherein the priming first expansion is performed in a container comprising a first gas-permeable surface area, wherein the priming first expansion is performed for first period of about 1 to 7 / 8 days to obtain the second population of TILs, wherein the second population of TILs is greater in number than the first population of TILs;(e) performing a rapid second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and APCs, to produce a third population of TILs, wherein the number of APCs added in the rapid second expansion is at least twice the number of APCs added in step (b), wherein the rapid second expansion is performed for a second period of about 1 to 11 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the rapid second expansion is performed in a container comprising a second gas-permeable surface area;(f) harvesting therapeutic population of TILs obtained from step (f), wherein the transition from step (e) to step (f) occurs without opening the system; and(g) transferring the harvested TIL population from step (g) to an infusion bag, wherein the transfer from step (1) to (g) occurs without opening the system; and(h) cryopreserving the infusion bag comprising the harvested TIL population from step (g) using a cry opreservation process. . The method of claim 104, wherein the rapid expansion can proceed for 1 day, 2 days, 3 days, 4, days, 5 days, 6 days, 7 days, 8 days, 9 days or 10 days after initiation of the rapid expansion.. The method of any of the preceding claims, wherein the IL-2 is present at an initial concentration of between 1000 lU / mL and 6000 lU / mL in the first cell culture medium, when present. . The method of any of the preceding claims, wherein the IL-2 is present at an initial concentration of between 1000 lU / mL and 6000 lU / mL and the OKT-3 antibody is present at aninitial concentration of about 30 ng / mL in the second wherein the initial expansion, when present, is performed using a gas permeable container. . The method of any of the preceding claims, wherein the rapid expansion, when present, is performed using a gas permeable container. . The method of any of the preceding claims, wherein the first cell culture medium, when present, further comprises a cytokine selected from the group consisting of IL-4, IL-7, IL-15, IL- 21, and combinations thereof. . The method of any of the preceding claims, wherein the second cell culture medium, when present, further comprises a cytokine selected from the group consisting of IL-4, IL-7, IL-15, IL- 21, and combinations thereof. . The method according to any of the preceding claims, wherein the harvested population of TILs comprises a therapeutically effective population of TILs, and wherein a therapeutically effective population of TILs comprises from about 2.3x IO10to about 13.7* IO10TILs. . The method of any of the preceding claims, wherein the initial expansion is performed over a period of 21 days or less. . The method of any of the preceding claims, wherein the initial expansion is performed over a period of 7 days or less. . The method of any of the preceding claims, wherein the rapid expansion is performed over a period of 7 days or less. . The method of any of the preceding claims, wherein the first expansion and the second expansion arc each individually performed within a period of 11 days. . The method of any of the preceding claims, wherein step of performing the first expansion through the setp of harvesting are performed in about 10 days to about 24 days. . The method of any of the preceding claims, wherein step of performing the first expansion through the setp of harvesting are performed in about 10 days to about 22 days. . The method of any of the preceding claims, further comprising a step of modifying a portion of the TILs at any time prior to the harvesting such that each of the modified TILs comprises an immunomodulatory composition associated with its surface membrane. . The method of any of the preceding claims, wherein the step of modifying comprises geneediting the TILs to effect transfer of at least one gene editing system selected from the group consisting of a TALEN system, a CRISPR system and zinc finger system, into the modified TILs.. The method of any of the preceding claims, wherein during the step of modifying the cellmembranes of the modified TILs are temporarily disrupted to effect transfer of the at least one gene editing system by using a microfluidic platform or sterile electroporation. . The method of Claim 120, wherein the microfluidic platform is a SQZ platform. . The method of any of the preceding claims, wherein immunomodulatory composition comprises one or more membrane anchored immunomodulatory fusion proteins each comprising one or more immunomodulatory agents and a cell membrane anchor moiety. . The method of any of the preceding claims, wherein the immunomodulatory composition comprises two or more different membrane anchored immunomodulatory fusion proteins, wherein each of the different membrane anchored immunomodulatory fusion proteins each comprises a different immunomodulatory agent. . The method of any of the preceding claims, wherein immunomodulatory composition comprises a fusion protein comprising one or more immunomodulatory agents linked to a TIL surface antigen binding domain. . The method of any of the preceding claims, wherein immunomodulatory composition comprises a nanoparticle comprising a plurality of immunomodulatory agents. . The method of any of the preceding claims, wherein the immunomodulatory agents comprise one or more cytokines. . The method of claim 126, wherein the one or more cytokines comprise IL-2, IL-6, IL-7, IL-9, IL-12, IL-15, IL-18, IL-21, IL-23, IL-27, IFN gamma, TNFa, IFN alpha, IFN beta, GM-CSF, or GCSF or a variant thereof. . The method of any of the preceding claims, wherein the immunomodulatory agents comprise a CD40 agonist. . The method of any of the preceding claims, wherein the membrane anchored immunomodulatory fusion protein is according to the formula, from N- to C-terminus: S-IA-L-C, wherein S is a signal peptide, IA is an immunomodulatory agent, L is a linker and C is a cell membrane anchor moiety. . The method of any of the preceding claims, wherein the cell membrane anchor moiety comprises a CD8a transmembrane-intracellular domain, a B7-1 transmembrane domain, a B7-2 transmembrane domain, or a CD8a transmembrane domain. . The method of any of the preceding claims, wherein the one or more immunomodulatory agents comprise one or more cytokines. . The method of any of the preceding claims, wherein the one or more cytokines comprises IL-2, IL-6, IL-7, IL-9, IL-12, IL-15, IL-18, IL-21, IL-23, IL-27, IFN gamma, TNFa, IFN alpha, IFN beta, GM-CSF, or GCSF or a variant thereof. . The method of any of the preceding claims, wherein the TIL surface antigen binding domain comprises an antibody variable heavy domain and variable light domain. . The method of any of the preceding claims, wherein the TIL surface antigen binding domain comprises an antibody or fragment thereof. . The method of any of the preceding claims, wherein the TIL surface antigen binding domain exhibits an affinity for one or more of following TIL surface antigens: CD45, CD4, CD8, CD3, CDlla, CDllb, CDllc, CD18, CD25, CD127, CD19, CD20, CD22, HLA- DR, CD197, CD38, CD27, CD196, CXCR3, CXCR4, CXCR5, CD84, CD229, CCR1, CCR5, CCR4, CCR6, CCR8, CCR10, CD 16, CD56, CD 137, 0X40, or GITR . The method of any of the preceding claims, wherein the nanoparticle is a liposome, a protein nanogel, a nucleotide nanogel, a polymer nanoparticle, or a solid nanoparticle.. The method of claim 136, wherein the nanoparticle is a nanogel. . The method of any of the preceding claims, wherein the nanoparticle further comprises an antigen binding domain that binds to one or more of the following antigens: CD45, CDlla (integrin alpha- L), CD 18 (integrin beta-2), CDllb, CDllc, CD25, CD8, or CD4.. The method of any of the preceding claims, wherein the modifying comprises introducing a heterologous nucleic acid encoding the fusion protein into the portion of TILs and expressing the fusion protein on the surface of the modified TILs. . The method of claim 139, wherein the heterologous nucleic acid is introduced into the genome of the modified TIL using one or more methods selected from a CRISPR method, a TALE method, a zinc finger method, and a combination thereof. . The method of any of the preceding claims, wherein the modifying comprises incubating the fusion protein with the portion of TILs under conditions to permit the binding of the fusion protein to the portion of TILs. . The method of any of the preceding claims, wherein the modifying comprises attaching the immunomodulatory composition to the surface of the portion of TILs. . The method according to any of the preceding claims, wherein the modifying is carried out on TILs from the first expansion, or TILs from the second expansion, or both. . The method according to any of the preceding claims, wherein the modifying is carried out onTILs from the priming first expansion, or TILs from the rapid second expansion, or both. . The method according to any of the preceding claims, wherein the modifying is carried out after the first expansion and before the second expansion. . The method according to any of the preceding claims, wherein the modify ing is carried out after the priming first expansion and before the rapid second expansion, or both. . The method according to any of the preceding claims, wherein the modifying is carried out after the second expansion. . The method according to any of the preceding claims, wherein the modify ing is carried out after the rapid second expansion. . The method according to any of the preceding claims, wherein the modify ing is carried out after the harvesting. . The method according to any of the preceding claims, wherein the modified TILs further comprise a genetic modification that causes expression of one or more immune checkpoint genes to be silenced or reduced in at least a portion of the therapeutic population of TILs. . The method according to claim 150, wherein said one or more immune checkpoint genes is / are selected from the group comprising PD-1, CTLA-4, LAG-3, HAVCR2 (TIM-3), Cish, TGFp, PKA, CBL-B, PPP2CA, PPP2CB, PTPN6, PTPN22, PDCD1, BTLA, CD 160, TIGIT, TET2, CD96, CRTAM, LAIR1, SIGLEC7, SIGLEC9, CD244, TNFRSF10B, TNFRSF10A, CASP8, CASP10, CASP3, CASP6, CASP7, FADD, FAS, SMAD2, SMAD3, SMAD4, SMAD10, SKI, SKIL, TGIF1, IL10RA, IL10RB, HMOX2, IL6R, IL6ST, EIF2AK4, CSK, PAG1, SIT1, FOXP3, PRDM1, BATF, GUCY1A2, GUCY1A3, GUCY1B2, GUCY1B3, TOX, SOCS1, ANKRD11, and BCOR. . The method according to claim 150, wherein said one or more immune checkpoint genes is / are selected from the group comprising PD-1, CTLA-4, LAG-3, HAVCR2 (TIM-3), Cish, TGFp, and PKA. . The method according to any of the preceding claims, wherein the modified TILs further comprises a genetic modification that causes expression of one or more immune checkpoint genes to be enhanced in at least a portion of the therapeutic population of TILs, the immune checkpoint gcnc(s) being selected from the group comprising CCR2, CCR4, CCR5, CXCR2, CXCR3, CX3CR1, IL-2, IL-4, IL-7, IL-10, IL-15, IL-21, the NOTCH 1 / 2 intracellular domain (ICD), and / or the NOTCH ligand mDLLl.. The method according to any of the preceding claims, wherein the genetic modification is produced using a programmable nuclease that mediates the generation of a double-strand or single-strand break at said one or more immune checkpoint genes. . The method according to any of the preceding claims, wherein the genetic modification is produced using one or more methods selected from a CRISPR method, a TALE method, a zinc finger method, and a combination thereof. . The method of any of any of the preceding claims, wherein the modified TILs are modified to transiently express the immunomodulatory composition on the cell surface. . The method of claim 156, wherein the immunomodulatory composition comprises one or more membrane anchored immunomodulatory fusion proteins, wherein each fusion protein comprises one or more immunomodulatory agents and a cell membrane anchor moiety. . The method of any of the preceding claims, wherein the modified TILs are modified by transfecting the TILs with a nucleic acid encoding the fusion protein. . The method of claim 158, wherein the nucleic acid is an RNA. . The method of claim 159, wherein the RNA is a mRNA. . The method of claim 160, wherein the TILs are transfected with the mRNA by a SQZ platform or electroporation. . The method of claim 161, wherein the TILs are transfected with the mRNA by a SQZ platform or electroporation after the first expansion and before the second expansion. . The method of claim 161, wherein the TILs are transfected with the mRNA by a SQZ platform or electroporation before the first expansion. . The method of claim 160, wherein the modified TILs are transfected with the nucleic acid encoding the fusion protein using a microfluidic device to temporarily disrupt the cell membranes of the TILs, thereby allowing transfection of the nucleic acid. . The method of any of the preceding claims, wherein the method further comprises activating the TILs by incubation with an anti-CD3 agonist before transfecting the TILs with the mRNA.. The method of claim 165, wherein the anti-CD3 agonist is OKT-3. . The method of claim 165 or 166, wherein the TILs are activated by incubating the TILs with the anti-CD3 agonist for about 1 to 3 days before transfecting the TILs with the mRNA. . A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) cry opreserving the tumor tissue comprising the first population of TILs from step (a) to produce a cryopreserved tumor tissue;(c) thawing the cryopreserved tumor tissue and culturing the first population of TILs in a first cell culture medium comprising IL-2 for about 3-9 days to produce a second population of TILs;(d) activating the second population of TILs using anti-CD3 agonist beads or antibodies, or anti-CD3 agonist and anti-CD28 agonist beads or antibodies, for 1-7 days, to produce a third population of TILs;(e) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs; and(f) culturing the fourth population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 5-15 days, to produce an expanded number of TILs. . A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) digesting in an enz me media the tumor tissue to produce a tumor digest;(c) cry opreserving the tumor digest comprising the first population of TILs from step (a) to produce a cryopreserved tumor digest;(d) thawing the cryopreserved tumor digest and culturing the first population of TILs in a first cell culture medium comprising IL-2 for about 3-9 days to produce a second population of TILs;(e) activating the second population of TILs using anti-CD3 agonist beads or antibodies, or anti-CD3 agonist and anti-CD28 agonist beads or antibodies, for 1-7 days, to produce a third population of TILs;(f) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs; and(g) culturing the fourth population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 5-15 days, to produce an expanded number of TILs.

170. The method of any of the preceding claims, wherein the enzymatic media comprises a DNase.

171. The method of any of the preceding claims, wherein the enzymatic media comprises a collagenase.

172. The method of any of the preceding claims, wherein the enzymatic media comprises a neutral protease.

173. The method of any of the preceding claims, wherein the enzymatic media comprises a hyaluronidase.

174. A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) cry opreserving the tumor tissue comprising the first population of TILs from step (a) to produce a cryopreserved tumor tissue;(c) thawing the cryopreserved tumor tissue and culturing the first population of TILs in a first cell culture medium comprising IL-2 for about 3-9 days to produce a second population of TILs;(d) activating the second population of TILs using anti-CD3 agonist beads or antibodies, or anti-CD3 agonist and anti-CD28 agonist beads or antibodies, for 1-7 days, to produce a third population of TILs;(e) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs;(f) culturing the fourth population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 1-7 days, to produce a culture of a fifth population of TILs; and(g) splitting die culture of the fifth population of TILs into a plurality of subcultures, culturing each of the plurality of subcultures in a third cell culture medium comprising IL-2 for about 3- 6 days, and combining the plurality of subcultures to provide an expanded number of TILs.

175. A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) digesting in an enzyme media the tumor tissue to produce a tumor digest;(c) cry opreserving the tumor digest comprising the first population of TILs from step (a) to produce a cryopreserved tumor digest;(d) thawing the cryopreserved tumor digest and culturing the first population of TILs in a first cell culture medium comprising IL-2 for about 3-9 days to produce a second population of TILs;(e) activating the second population of TILs using anti-CD3 agonist beads or antibodies, or anti-CD3 agonist and anti-CD28 agonist beads or antibodies, for 1-7 days, to produce a third population of TILs;(f) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs;(g) culturing the fourth population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 1-7 days, to produce a culture of a fifth population of TILs; and(h) splitting the culture of the fifth population of TILs into a plurality of subcultures, culturing each of the plurality of subcultures in a third cell culture medium comprising IL-2 for about 3- 6 days, and combining the plurality of subcultures to provide an expanded number of TILs.

176. A method of making a cry opreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) cry opreserving the tumor tissue comprising the first population of TILs from step (a) to produce a cryopreserved tumor tissue;(c) thawing the cryopreserved tumor tissue and culturing the first population of TILs in a first cell culture medium comprising IL-2 and OKT-3 for about 3-9 days to produce a second population of TILs;(d) gene-editing at least a portion of the second population of TILs, to produce a third population of TILs; and(c) culturing the third population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 5-15 days, to produce an expanded number of TILs.

177. A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) digesting in an enzyme media the tumor tissue to produce a tumor digest;(c) cry opreserving the tumor digest comprising the first population of TILs from step (a) to produce a cryopreserved tumor digest;(d) thawing the cryopreserved tumor digest and culturing the first population of TILs in a first cell culture medium comprising IL-2 and OKT-3 for about 3-9 days to produce a second population of TILs;(e) gene-editing at least a portion of the second population of TILs, to produce a third population of TILs; and(f) culturing the third population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 5-15 days, to produce an expanded number of TILs. . A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) cry opreserving the tumor tissue comprising the first population of TILs from step (a) to produce a cryopreserved tumor tissue;(c) thawing the cryopreserved tumor tissue and culturing the first population of TILs in a first cell culture medium comprising IL-2 and OKT-3 for about 3-9 days to produce a second population of TILs;(d) gene-editing at least a portion of the second population of TILs, to produce a third population of TILs;(e) culturing the third population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 1-7 days, to produce a culture of a fourth population of TILs; and(f) splitting the culture of the fourth population of TILs into a plurality of subcultures, culturing each of the plurality of subcultures in a third cell culture medium comprising IL-2 for about 3-7 days, and combining the plurality of subcultures to provide a fifth population of TILs comprising an expanded number of TILs. . A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) digesting in an enzyme media the tumor tissue to produce a tumor digest;(c) cry opreserving the tumor digest comprising the first population of TILs from step (a) to produce a cryopreserved tumor digest;(d) thawing the cryopreserved tumor digest and culturing the first population of TILs in a first cell culture medium comprising IL-2 and OKT-3 for about 3-9 days to produce a second population of TILs;(e) gene-editing at least a portion of the second population of TILs, to produce a third population of TILs;(f) culturing the third population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 1-7 days, to produce a culture of a fourth population of TILs; and(g) splitting the culture of the fourth population of TILs into a plurality of subcultures, culturing each of the plurality of subcultures in a third cell culture medium comprising IL-2 for about 3-7 days, and combining the plurality of subcultures to provide a fifth population of TILs comprising an expanded number of TILs. . A method of making a cry opreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) cry opreserving the tumor tissue comprising the first population of TILs from step (a) to produce a cryopreserved tumor tissue;(c) thawing the cryopreserved tumor tissue and culturing the first population of TILs in a first cell culture medium comprising IL-2 for about 3 days to produce a second population of TILs;(d) culturing the second population of TILs in a second cell culture medium comprising IL-2 and OKT-3 for 2-4 days to produce a third population of TILs;(e) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs; and(f) culturing the fourth population of TILs in a third cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 5-15 days, to produce an expanded number of TILs. . A method of making a cryopreserved population of TILs from a cancer patient, wherein thepatient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) digesting in an enzyme media the tumor tissue to produce a tumor digest;(c) cry opreserving the tumor digest comprising the first population of TILs from step (a) to produce a cryopreserved tumor digest;(d) thawing the cryopreserved tumor digest and culturing the first population of TILs in a first cell culture medium comprising IL-2 for about 3 days to produce a second population of TILs;(e) culturing die second population of TILs in a second cell culture medium comprising IL-2 and OKT-3 for 2-4 days to produce a third population of TILs;(f) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs; and(g) culturing the fourth population of TILs in a third cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 5-15 days, to produce an expanded number of TILs. . A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) cry opreserving the tumor tissue comprising the first population of TILs from step (a) to produce a cryopreserved tumor tissue;(c) thawing the cryopreserved tumor tissue and culturing the first population of TILs in a first cell culture medium comprising IL-2 for about 3 days to produce a second population of TILs;(d) culturing the second population of TILs in a second cell culture medium comprising IL-2 and OKT-3 for 2-4 days to produce a third population of TILs;(e) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs;(f) culturing the third population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 1-7 days, to produce a culture of a fourth population of TILs; and(g) splitting the culture of the fourth population of TILs into a plurality of subcultures, culturing each of the plurality of subcultures in a third cell culture medium comprising IL-2 for about 3-7 days, and combining tire plurality of subcultures to provide a fifth population of TILs comprising an expanded number of TILs. . A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) digesting in an enz me media the tumor tissue to produce a tumor digest;(c) cry opreserving the tumor digest comprising the first population of TILs from step (a) to produce a cryopreserved tumor digest;(d) thawing the cryopreserved tumor digest and culturing the first population of TILs in a first cell culture medium comprising IL-2 for about 3 days to produce a second population of TILs;(e) culturing the second population of TILs in a second cell culture medium comprising IL-2 and OKT-3 for 2-4 days to produce a third population of TILs;(f) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs;(g) culturing the fourth population of TILs in a second cell culture medium comprising antigen presenting cells (APCs), OKT-3, and IL-2 for about 1-7 days, to produce a culture of a fifth population of TILs; and(h) splitting the culture of the fifth population of TILs into a plurality of subcultures, culturing each of the plurality of subcultures in a third cell culture medium comprising IL-2 for about 3- 6 days, and combining the plurality of subcultures to provide a fifth population of TILs comprising an expanded number of TILs. . A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) cry opreserving the tumor tissue comprising the first population of TILs from step (a) to produce a cryopreserved tumor tissue;(c) thawing the cryopreserved tumor tissue and performing an initial expansion (or primingfirst expansion) of the first population of TILs in a first cell culture medium to obtain a second population of TILs, wherein the first cell culture medium comprises IL-2, optionally OKT-3, and optionally antigen presenting cells (APCs), wherein the priming first expansion occurs for a period of about 3 to 8 days;(d) activating the second population of TILs using anti-CD3 agonist beads or antibodies, or anti-CD3 agonist and anti-CD28 agonist beads or antibodies, for 1-6 days, to produce a third population of TILs;(e) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs;(f) performing a rapid second expansion of the fourth population of TILs in a second cell culture medium to obtain an expanded number of TILs, wherein the second cell culture medium comprises IL-2, OKT-3, and APCs; and wherein the rapid expansion is performed over a period of 14 days or less, optionally the rapid second expansion can proceed for about 1 day, 2 days, 3 days, 4, days, 5 days, 6 days, 7 days, 8 days, 9 days or 10 days after initiation of the rapid second expansion. . A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) cry opreserving the tumor tissue comprising the first population of TILs from step (a) to produce a cryopreserved tumor tissue;(c) subsequent to the cancer progression, performing an initial expansion (or priming first expansion) of the first population of TILs in a first cell culture medium to obtain a second population of TILs, wherein the first cell culture medium comprises IL-2, optionally OKT-3, and optionally antigen presenting cells (APCs), wherein the priming first expansion occurs for a period of about 3 to 8 days;(d) gene-editing at least a portion of the second population of TILs, to produce a third population of TILs; and(e) performing a rapid second expansion of the third population of TILs in a second cell culture medium to obtain an expanded number of TILs, wherein the second cell culture medium comprises IL-2, OKT-3, and APCs; and wherein the rapid expansion is performed over a period of 14 days or less, optionally the rapid second expansion can proceed for about1 day, 2 days, 3 days, 4, days, 5 days, 6 days, 7 days, 8 days, 9 days or 10 days after initiation of the rapid second expansion. . A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) digesting in an enz me media the tumor fragments to produce a tumor digest;(c) cry opreserving the tumor digest comprising the first population of TILs from step (a) to produce a cryopreserved tumor digest;(d) thawing the cryoprcscrvcd tumor digest and performing an initial expansion (or priming first expansion) of the first population of TILs in a first cell culture medium to obtain a second population of TILs, wherein the first cell culture medium comprises IL-2, optionally OKT-3, and optionally antigen presenting cells (APCs), where the pruning first expansion occurs for a period of about 1 to 8 days;(e) activating the second population of TILs using anti-CD3 agonist beads or antibodies, or anti-CD3 agonist and anti-CD28 agonist beads or antibodies, for 1-6 days, to produce a third population of TILs;(f) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs;(g) performing a rapid second expansion of the fourth population of TILs in a second cell culture medium to obtain an expanded number of TILs, wherein the second cell culture medium comprises IL-2, OKT-3, and APCs; and wherein the rapid expansion is performed over a period of 14 days or less, optionally the rapid second expansion can proceed for about 1 day, 2 days, 3 days, 4, days, 5 days, 6 days, 7 days, 8 days, 9 days or 10 days after initiation of the rapid second expansion. . A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a tumor tissue resected from a subject or patient;(b) digesting in an enzy me media the tumor fragments to produce a tumor digest;(c) cry opreserving the tumor digest comprising the first population of TILs from step (a) to produce a cryopreserved tumor digest;(d) thawing the cryopreserved tumor digest and performing an initial expansion (or pruning first expansion) of the first population of TILs in a first cell culture medium to obtain a second population of TILs, wherein the first cell culture medium comprises IL-2, optionally OKT-3, and optionally antigen presenting cells (APCs), where the priming first expansion occurs for a period of about 1 to 8 days;(e) gene-editing at least a portion of the second population of TILs, to produce a third population of TILs; and(f) performing a rapid second expansion of the third population of TILs in a second cell culture medium to obtain an expanded number of TILs, wherein the second cell culture medium comprises IL-2, OKT-3, and APCs; and wherein the rapid expansion is performed over a period of 14 days or less, optionally the rapid second expansion can proceed for about 1 day, 2 days, 3 days, 4, days, 5 days, 6 days, 7 days, 8 days, 9 days or 10 days after initiation of the rapid second expansion. . A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a maintaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a sample of tumor tissue produced by surgical resection, needle biopsy, core biopsy, small biopsy, or other means for obtaining tumor tissue from a patient or subject;(b) cry opreserving the tumor tissue comprising the first population of TILs from step (a) to produce a cryopreserved tumor tissue;(c) thawing the cryopreserved tumor tissue and adding the tumor tissue into a closed system and performing a first expansion by culturing the first population of TILs in a first cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-9 days to obtain the second population of TILs;(d) activating the second population of TILs using anti-CD3 agonist beads or antibodies, or CD3 agonist and CD28 agonist beads or antibodies, for 1-7 days, to produce a third population of TILs;(e) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs;(f) performing a second expansion by culturing the fourth population of TILs in a second cell culture medium comprising IL-2, OKT-3, and antigen presenting cells (APCs), to produce a fifth population of TILs, wherein the second expansion is performed for about 5-15 days to obtain the fifth population of TILs, wherein the second expansion is performed in a closed container providing a second gas-permeable surface area, wherein the fifth population of TILs is a therapeutic population of TILs; and(g) harvesting the therapeutic population of TILs obtained from step (f), wherein each of steps(c) to (g) is performed in a closed, sterile system, and wherein the transition from step (c) to step (d), the transition from step (d) to step (e), the transition from step (e) to step (f) and / or the transition from step (I) to step (g) occurs without opening the system. . A method of making a cryopreserved population of TILs from a cancer patient, wherein the patient is naive to a cancer treatment or on a mamtaience therapy that is interrupted, the method comprising:(a) prior to the cancer treatment or resumption of the maintenance therapy, obtaining and / or receiving a first population of TILs from a sample of tumor tissue produced by surgical resection, needle biopsy, core biopsy, small biopsy, or other means for obtaining tumor tissue from a patient or subject;(b) digesting the sample of tumor tissue in an enzymatic media to produce a tumor digest;(c) cry opreserving the tumor digest comprising the first population of TILs to produce a cryopreserved tumor digest;(d) thawing the cryopreserved tumor digest and adding the tumor digest into a closed system and performing a first expansion by culturing the first population of TILs in a first cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for about 3-9 days to obtain the second population of TILs;(d) activating the second population of TILs using anti-CD3 agonist beads or antibodies, or CD3 agonist and CD28 agonist beads or antibodies, for 1-7 days, to produce a third population of TILs;(e) gene-editing at least a portion of the third population of TILs, to produce a fourth population of TILs;(I) performing a second expansion by culturing the fourth population of TILs in a second cell culture medium comprising IL-2, OKT-3, and antigen presenting cells (APCs), to produce a fifth population of TILs, wherein the second expansion is performed for about 5-15 days to obtain the fifth population of TILs, wherein the second expansion is performed in a closedcontainer providing a second gas-permeable surface area, wherein the fifth population of TILs is a therapeutic population of TILs; and(g) harvesting the therapeutic population of TILs obtained from step (f), wherein each of steps (d) to (g) is performed in a closed, sterile system, and wherein the transition from step (d) to step (e), the transition from step (e) to step (f), and / or the transition from step (f) to step (g) occurs without opening the system. . The method of any of the preceding claims, wherein the rapid second expansion is performed by culturing the third or fourth population of TILs in the second cell culture medium for a first period of about 1-7 days, at the end of the first period the culture is split into a plurality of subcultures, each of the plurality of subcultures is cultured in a third culture medium comprising IL-2 for a second period of about 3-6 days, and at the end of the second period the plurality of subcultures are combined to provide the expanded number of TILs. . The method of any of the preceding claims, wherein the step of gene-editing at least a portion of die second or third population of TILs comprises performing a sterile electroporation step or a SQZ microfluidic membrane disruption step on the second or third population of TILs, wherein the sterile electroporation step or the SQZ microfluidic membrane disruption step mediates the transfer of at least two gene editors. . The method of claim 191, wherein the electroporation step or the SQZ microfluidic membrane disruption step consists of a single event that mediates the transfer of the at least two gene editors.. The method of any of the preceding claims, wherein in the electroporation step or the SQZ microfluidic membrane disruption step each of the at least two gene editors is transferred individually by an event independently of the transfer of any other gene editor. . The method of any of the preceding claims, wherein the electroporation step further comprises a rest period after each event. . The method of any of the preceding claims, wherein the electroporation step or the SQZ microfluidic membrane disruption step comprises a first event that mediates the transfer of a first gene editor for modulating expression of a first protein, a first rest period, a second event that mediates the transfer of a second gene editor for modulating expression of a second protein, and a second rest period, w herein the first and second rest periods are the same or different. . The method of any of the preceding claims, wherein the first and second rest periods comprise incubating the third or fourth population of TILs in a cell culture medium comprising IL-2.. The method of any of the preceding claims, wherein the first and second rest periods comprise incubating the third or fourth population of TILs at about 30-40 °C with about 5% CO2.. The method of any of the preceding claims, wherein the first and second rest periods are independently about 10 hours to 5 days. . The method of any of the preceding claims, wherein the first and second rest periods are independently about 10 hours to 3 days. . The method of any of the preceding claims, wherein the first rest period is about 1 to 3 days.. The method of any of the preceding claims, wherein the first rest period is about 3 days.. The method of any of any of the preceding claims, wherein the second rest period is about 10 hours to 1 day. . The method of any of any of the preceding claims, wherein the second rest period is about 12 hours to 24 hours. . The method of any of any of the preceding claims, wherein the second rest period is about 15 hours to about 18 hours. . The method of any of any of the preceding claims, wherein the second rest period comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2 for about 15 hours to 23 hours at about 30°C. . The method of any of any of the preceding claims, wherein the second rest period comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2 for about for about one horn at 37°C followed by about 15 hours to 23 hours at about 30°C. . The method of any of any of the preceding claims, wherein the second rest period comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2 for about for about one hour at 37°C followed by about 15 hours to 22 hours at about 30°C. . The method of claim 207, wherein the first rest period is about 3 days and the second rest period is about 10 to 16 hours. . The method of any of the preceding claims, wherein the at least two gene editors comprise a first gene editor comprising a first TALE nuclease system for modulating expression of a first protein and a second gene editor comprising a second TALE nuclease system for modulating expression of a second protein. . The method of any of the preceding claims, wherein the electroporation step or the SQZ microfluidic membrane disruption step comprises a first event that mediates the transfer of the first TALE nuclease system, a first rest period, a second event that mediates the transfer of the second TALE nuclease system, and a second rest period, wherein the first and second rest periods are the same or different.. The method of any of the preceding claims, wherein the first and second rest periods comprise incubating the third or fourth population of TILs in a cell culture medium comprising IL-2.. The method of any of the preceding claims, wherein the first and second rest periods comprise incubating the third or fourth population of TILs at about 30-40 °C with about 5% CO2. . The method of any of the preceding claims, wherein the first and second rest periods are independently about 10 hours to 5 days. . The method of any of the preceding claims, wherein the first and second rest periods are independently about 10 hours to 3 days. . The method of any of the preceding claims, wherein the first rest period is about 1 to 3 days.. The method of any of the preceding claims, wherein the first rest period is about 3 days.. The method of any of the preceding claims, wherein the second rest period is about 10 hours to1 day. . The method of any of the preceding claims, wherein the second rest period is about 12 hours to 24 horns. . The method of any of the preceding claims, wherein the second rest period is about 15 horns to about 18 hours. . The method of any of the preceding claims, wherein the second rest period comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2 for about 15 hours to 23 hours at about 30°C. . The method of any of the preceding claims, wherein the second rest period comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2 for about for about one hour at 37°C followed by about 15 hours to 23 hours at about 30°C. . The method of any of the preceding claims, wherein the second rest period comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2 for about for about one hour at 37°C followed by about 15 hours to 22 horns at about 30°C. . The method of any of the preceding claims, wherein the first rest period is about 3 days and the second rest period is about 10 to 16 hours. . The method of any of the preceding claims, wherein the step of gene-editing at least a portion of the second or third population of TILs comprises performing a sterile electroporation step or a SQZ microfluidic membrane disruption step on the second or third population of TILs, wherein the sterile electroporation step or the SQZ microfluidic membrane disruption step mediates the transfer of at least one gene editor.. The method of any of the preceding claims, wherein the gene editor is a TALE nuclease system for modulating the expression of at least one protein. . The method of any of the preceding claims, wherein the TALE nuclease system modulates expression of PD-1, CTLA-4, TIGIT, CBL-B and / or LAG-3. . The method of any of the preceding claims, wherein the gene editor comprises a TALE nuclease system that modulates expression of PD-1 and CTLA-4. . The method of any of the preceding claims, wherein the gene editor comprises a TALE nuclease system that modulates expression of PD-1 and LAG-3. . The method of any of the preceding claims, wherein the gene editor comprises a TALE nuclease system that modulates expression of PD-1 and CISH. . The method of any of the preceding claims, wherein the gene editor comprises a TALE nuclease system that modulates expression of PD-1 and CBL-B. . The method of any of the preceding claims, wherein the gene editor comprises a TALE nuclease system that modulates expression of PD-1 and TIGIT. . The method of any of the preceding claims, wherein the gene editor comprises a TALE nuclease system that modulates expression of CTLA-4 and LAG-3. . The method of any of the preceding claims, wherein the gene editor comprises a TALE nuclease system that modulates expression of CTLA-4 and CISH. . The method of any of the preceding claims, wherein the gene editor comprises a TALE nuclease system that modulates expression of CTLA-4 and CBL-B. . The method of any of the preceding claims, wherein the gene editor comprises a TALE nuclease system that modulates expression of CISH and CBL-B. . The method of any of the preceding claims, wherein the gene editor comprises a TALE nuclease system that modulates expression of CISH and TIGIT. . The method of any of the preceding claims, wherein the gene editor comprises a TALE nuclease system that modulates expression of PD-1. . The method of any of the preceding claims, wherein the gene editor comprises a TALE nuclease system that modulates expression of CTLA-4. . The method of any of the preceding claims, wherein the gene editor comprises a TALE nuclease system that modulates expression of LAG-3. . The method of any of the preceding claims, wherein the gene editor comprises a TALEnuclease system that modulates expression of CISH. . The method of any of the preceding claims, wherein the gene editor comprises a TALE nuclease system that modulates expression of CBL-B. . The method of any of the preceding claims, wherein the gene editor comprises a TALE nuclease system that modulates expression of TIGIT. . The method of any of the preceding claims, wherein the method further comprises the step of resting the third or fourth population of TILs after the gene-editing step and before the step of culturing the third or fourth population of TILs. . The method of any of the preceding claims, wherein the resting step comprises incubating the third or fourth population of TILs at about 30-40 °C with about 5% CO2. . The method of any of the preceding claims, wherein the method further comprises the step of resting the third or fourth population of TILs for about one day after the gene-editing step and before the step of culturing the third or fourth population of TILs. . The method of any of the preceding claims, wherein the method further comprises the step of resting the third or fourth population of TILs for about 12 hours to 24 hours after the gene-editing step and before the step of culturing the third or fourth population of TILs. . The method of any of the preceding claims, wherein the step of resting the third or fourth population of TILs comprises resting the third or fourth population of TILs for about 15 hours to 18 horns. . The method of any of the preceding claims, wherein the step of resting the third or fourth population of TILs comprises resting the third or fourth population of TILs for about 15 horns.. The method of any of the preceding claims, wherein the step of resting the third or fourth population of TILs comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2. . The method of any of the preceding claims, wherein the step of resting the third or fourth population of TILs comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2 for about 15 hours to 23 hours at about 30°C. . The method of any of the preceding claims, wherein the step of resting third or fourth population of TILs comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2 for about one hour at 37°C followed by about 15 hours to 23 hours at about 30°C. . The method of any of the preceding claims, wherein the step of resting the third or fourthpopulation of TILs comprises incubating the third or fourth population of TILs in a cell culture medium comprising IL-2 for about one horn at 37°C followed by about 15 hours to 22 hours at about 30°C. . The method of any of the preceding claims, wherein the first protein and the second protein are independently selected from the group consisting ofPD-1, CTLA-4, LAG-3, CISH, TIGIT and CBL-B, with the proviso that the first protein and the second protein are different. . The method of any of the preceding claims, wherein the first protein and the second protein are selected from the group consisting ofPD-1 and CTLA-4. . The method of any of the preceding claims, wherein the first protein and the second protein are selected from the group consisting ofPD-1 and LAG-3. . The method of any of the preceding claims, wherein the first protein and the second protein arc selected from the group consisting ofPD-1 and CISH. . The method of any of the preceding claims, wherein the first protein and the second protein are selected from the group consisting ofPD-1 and CBL-B. . The method of any of the preceding claims, wherein the first protein and the second protein are selected from the group consisting ofPD-1 and TIGIT. . The method of any of the preceding claims, wherein the first protein and the second protein are selected from the group consisting of CTLA-4 and LAG-3. . The method of any of the preceding claims, wherein the first protein and the second protein are selected from the group consisting of CTLA-4 and CISH. . The method of any of the preceding claims, wherein the first protein and the second protein are selected from the group consisting of CTLA-4 and CBL-B . The method of any of the preceding claims, wherein the first protein and the second protein are selected from the group consisting of LAG-3 and CISH. . The method of any of the preceding claims, wherein the first protein and the second protein are selected from the group consisting of LAG-3 and CBL-B. . The method of any of the preceding claims, wherein the first protein and the second protein are selected from the group consisting of CISH and CBL-B. . The method of any of the preceding claims, wherein the first protein is PD-1 and the second protein is CTLA-4. . The method of any of the preceding claims, wherein the first protein is CTLA-4 and the second protein is PD-1.

267. The method of any of the preceding claims, wherein the first protein is PD-1 and the second protein is LAG-3.

268. The method of any of the preceding claims, wherein the first protein is LAG-3 and the second protein is PD-1.

269. The method of any of the preceding claims, wherein the first protein is PD-1 and the second protein is CISH.

270. The method of any of the preceding claims, wherein the first protein is CISH and the second protein is PD-1.

271. The method of any of the preceding claims, wherein the first protein is PD-1 and the second protein is CBL-B.Til. The method of any of the preceding claims, wherein the first protein is CBL-B and the second protein is PD-1.

273. The method of any of the preceding claims, wherein the first protein is PD-1 and the second protein is TIGIT.

274. The method of any of the preceding claims, wherein the first protein is TIGIT and die second protein is PD-1.

275. The method of any of the preceding claims, wherein the first protein is CTLA-4 and the second protein is LAG-3.

276. The method of any of the preceding claims, wherein the first protein is LAG-3 and the second protein is CTLA-4.Til . The method of any of the preceding claims, wherein the first protein is CTLA-4 and the second protein is CISH.

278. The method of any of the preceding claims, wherein the first protein is CISH and the second protein is CTLA-4.

279. The method of any of the preceding claims, wherein the first protein is CTLA-4 and the second protein is CBL-B.

280. The method of any of the preceding claims, wherein the first protein is CBL-B and the second protein is CTLA-4.

281. The method of any of the preceding claims, wherein the first protein is LAG-3 and the second protein is CISH.

282. The method of any of the preceding claims, wherein the first protein is CISH and the secondprotein is LAG-3. . The method of any of the preceding claims, wherein the first protein is LAG-3 and the second protein is CBL-B. . The method of any of the preceding claims, wherein the first protein is CBL-B and the second protein is LAG-3. . The method of any of the preceding claims, wherein the first protein is CISH and the second protein is CBL-B. . The method of any of the preceding claims, wherein the first protein is CBL-B and the second protein is CISH. . The method of any of the preceding claims, wherein the first protein or the second protein is PD-1. . The method of any of the preceding claims, wherein the first protein or the second protein is CTLA-4. . The method of any of the preceding claims, wherein the first protein or the second protein is LAG-3. . The method of any of the preceding claims, wherein the first protein or the second protein is CISH. . The method of any of the preceding claims, wherein the first protein or the second protein is CBL-B. . The method of any of the preceding claims, wherein the first protein or the second protein is TIGIT. . The method of any of the preceding claims, wherein the first gene editor downregulates expression of the first protein and the second gene editor downregulates expression of the second protein. . The method of any of the preceding claims, wherein the expanded number of TILs comprises a therapeutic population of TILs. . The method of any of the preceding claims, wherein the antigen presenting cells (APCs) are PBMCs. . The method of any of the preceding claims, wherein the PBMCs are irradiated and allogeneic.. The method according to any of the preceding claims, wherein the antigen-presenting cells are artificial antigen-presenting cells.. The method according to any of the preceding claims, wherein the IL-2 concentration is about 10,000 lU / mL to about 5,000 lU / mL. . The method according to any of the preceding claims, wherein the first cell culture medium and / or the second cell culture medium further comprises a 4-1BB agonist and / or an 0X40 agonist. . The method of any of the preceding claims, wherein the patient is naive to an ICI treatment, an anti-VEGF treatment, a chemotherapeutic treatment, or combinations thereof. . The method of any of the preceding claims, wherein the patient is naive to any cancer treatment. . The method of any of the preceding claims, wherein the patient receives a first-line ICI treatment and / or standard treatment. . The method of any of the preceding claims, wherein the patient receives a second-line ICI treatment and / or standard treatment. . The method of any of the preceding claims, wherein step of thawing and the subsequent steps are performed after the patient receives the cancer treatment or resumes the maintenance therapy and exhibits progressive disease. . The method of any of the preceding claims, wherein the patient is in a washout period at initiation of step (a). . A method of treating a cancer patient by administering a population of tumor infiltrating lymphocytes (TILs) to the cancer patient in need thereof, wherein the cancer patient has received at least one prior therapy and has exhibited cancer progression on or after the at least one prior therapy, wherein obtaining the population of the TILs comprises multilesional sampling, wherein the population of TILs was made from a tumor sample harvested before the cancer patient receives the at least one prior therapy; and wherein the population of TILs is administered to the subject or patient after the cancer patient has exhibited cancer progression on or after the at least one prior therapy. . A method of treating a cancer patient by administering a population of tumor infiltrating lymphocytes (TILs) to the cancer patient in need thereof, wherein the cancer patient has received at least one prior therapy and has exhibited cancer progression on or after the at least one prior therapy, wherein obtaining the population of the TILs comprises multilesional sampling, wherein the population of TILs was made before the cancer patient receives the at least one prior therapy according to any of the preceding claims; and wherein the population of TILs is administered to the subject or patient after the cancerpatient has exhibited cancer progression on or after the at least one prior therapy. . The method of any of the preceding claims, wherein at the time of harvesting the tumor sample, the cancer patient is naive to any cancer treatment. . The method of any of the preceding claims, wherein at the time of harvesting the tumor sample, the patient is naive to ICI treatment, anti-VEGF treatment, or chemotherapeutic treatment. . The method of any of the preceding claims, wherein at the time of harvesting the tumor sample, the patient is on maintenance therapy that is interrupted at the time of step (a), and the maintenance therapy is resumed after step (a). . The method of any of the preceding claims, wherein at the time of harvesting the tumor sample, the patient is in a washout period at initiation of step (a). . The method of any of the preceding claims, wherein the patient receives or is going to receive a first-line ICI treatment and / or standard treatment. . The method of any of the preceding claims, wherein the patient receives or is going to receive a second-line ICI treatment and / or standard treatment. . The method of any of the preceding claims, further comprising administering an ICI to the cancer patient. . The method of any of the preceding claims, wherein the ICI is an inhibitor of a gene selected from the group consisting of PD-1, CTLA-4, LAG-3, HAVCR2 (TIM-3), Cish, TGF , PKA, CBL-B, PPP2CA, PPP2CB, PTPN6, PTPN22, PDCD1, BTLA, CD 160, TIGIT, TET2, BAFF (BR3), CD96, CRTAM, LAIR1, SIGLEC7, SIGLEC9, CD244, TNFRSF10B, TNFRSF10A, CASP8, CASP10, CASP3, CASP6, CASP7, FADD, FAS, SMAD2, SMAD3, SMAD4, SMAD10, SKI, SKIL, TGIF1, IL10RA, IL10RB, HMOX2, IL6R, IL6ST, EIF2AK4, CSK, PAG1, SIT1, FOXP3, PRDM1, BATF, GUCY1A2, GUCY1A3, GUCY1B2, GUCY1B3, TOX, SOCS1, ANKRD11, and BCOR. . The method of any of the preceding claims, further comprising the step of treating the patient with a non-myeloablative lymphodepletion regimen prior to administering the population of TILs to the patient. . The method of any of the preceding claims, wherein the non-myeloablative lymphodepletion regimen comprises the steps of administration of cyclophosphamide at a dose of 60 mg / m2 / day for two days followed by administration of fludarabine at a dose of 25 mg / m2 / day for five days.. The method of any of the preceding claims, wherein the non-myeloablative lymphodepletion regimen comprises the steps of administration of cyclophosphamide at a dose of 60 mg / m2 / dayand fludarabine at a dose of 25 mg / m2 / day for two days followed by administration of fludarabine at a dose of 25 mg / m2 / day for three days. . The method of any of the preceding claims, wherein the cyclophosphamide is administered with mesna. . The method of any of the preceding claims, further comprising the step of treating the patient with an IL-2 regimen starting on the day after administration of the population of TILs to the patient. . The method of any of the preceding claims, further comprising the step of treating the patient with an IL-2 regimen starting on the same day as administration of the population of TILs to the patient. . The method of any of the preceding claims, wherein the IL-2 regimen is a high-dose IL-2 regimen comprising 600,000 or 720,000 lU / kg of aldesleukin, or a biosimilar or variant thereof, administered as a 15-minute bolus intravenous infusion every eight hours until tolerance. . An expanded number of tumor infiltrating lymphocytes (TILs) or a therapeutic population of TILs manufactured by the method of any of the preceding claims. . A pharmaceutical composition comprising the expanded number of TILs or therapeutic population of TILs of any of the preceding claims and a pharmaceutically acceptable carrier.. A population of TILs manufactured using the method of any of the preceding claims. . A pharmaceutical composition comprising the population of TILs of claim 325. . The pharmaceutical composition of claim 326, further comprising an ICI. . The pharmaceutical composition of claim 327, wherein the ICI is an inhibitor of a gene selected from the group consisting of PD-1, CTLA-4, LAG-3, HAVCR2 (TIM -3), Cish, TGFp, PKA, CBL-B, PPP2CA, PPP2CB, PTPN6, PTPN22, PDCD1, BTLA, CD 160, TIGIT, TET2, BAFF (BR3), CD96, CRTAM, LAIR1, SIGLEC7, SIGLEC9, CD244, TNFRSF10B, TNFRSF10A, CASP8, CASP10, CASP3, CASP6, CASP7, FADD, FAS, SMAD2, SMAD3, SMAD4, SMAD10, SKI, SKIL, TGIF1, IL10RA, IL10RB, HMOX2, IL6R, IL6ST, EIF2AK4, CSK, PAG1, SIT1, FOXP3, PRDM1, BATF, GUCY1A2, GUCY1A3, GUCY1B2, GUCY1B3, TOX, SOCS1, ANKRD11, and BCOR. . A method of treating a cancer patient by administering the population of TILs of any of the preceding claims or the pharmaceutical composition of any of the preceding claims to the cancer patient, wherein the cancer patient has received at least one prior therapy, and the cancer patient exhibits cancer progression on or after the at least one prior therapy.

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