Closed device for culturing and transporting cell therapy product

Abstract

The present invention relates to a closed device (1) for culturing and transporting a cell therapy product, comprising: a container (2) with a bottom (20) having peripheral walls (21) mounted thereon, delimiting an internal volume with an opening (22) in the upper part; and a closure means (3), removably attached to cover said opening (22). The invention is characterised in that at least one of the peripheral walls (21) comprises at least two through-channels (4) opening into said internal volume. The invention also relates to a kit (7) equipped with such a culture device (1) and with a sampling member (8).

Description

[0001] CLOSED DEVICE FOR CULTIVATING AND TRANSPORTING CELL THERAPY PRODUCTS

[0002] TECHNICAL FIELD OF THE INVENTION

[0003] The present invention falls within the medical field of tissue culture. It should be remembered that tissue culture is carried out in vitro and consists of the growth of tissues or cells in a sterile artificial medium. Tissue culture generally involves a nutrient culture medium, in liquid, semi-solid or solid form.

[0004] The invention will find a preferential application in the culture of cell therapy products of the skin substitute type, such as the epidermis, composed of human pluripotent stem cells, more precisely based on keratinocytes, for example keratinocytes derived from pluripotent stem cells or based on melanocytes, or fibroblasts, or even endothelial cells. The invention also aims at a therapeutic application by grafting animal but preferably human skin, for example in the treatment of skin ulceration.

[0005] To this end, the invention relates to a device for culturing and transporting a cell therapy product, hereinafter referred to as a “culture device”.

[0006] STATE OF THE ART

[0007] Currently, cell culture involves a container, with a bottom and peripheral walls, forming an internal volume with an opening at the top, closed by a lid. This internal volume allows the culture medium to be deposited through the opening, then the cells to be cultured. Such a container is generally in the form of a Petri dish, cylindrical in shape, or more rarely rectangular in shape. A disadvantage of a Petri dish lies in the closure of its lid, without sealing, which allows the gas exchanges necessary for the good culture of the cells, but in no way ensures maintenance in a sterile atmosphere.

[0008] An alternative solution is a container in the form of a culture flask. Such a flask is generally shaped like a flask, with an upper opening sealed tightly by a lid. This flask also includes on one side wall an upwardly inclined neck, with a neck provided with a cap, which can be screwed from a hermetically sealed position to a venting position, and vice versa. Such a cap may also include a filter membrane, generally hydrophobic, protecting the interior of the flask from contaminants present in the external atmosphere.

[0009] More rarely, the said cover can be replaced by a peelable film, closing the opening hermetically. Such a film is applied by sealing, in particular heat-sealing, in contact with the edges or the edges of the peripheral walls around the said opening.

[0010] Further, these containers are made of plastic material, the characteristics of which allow sterilization, but also the good development of the cells to be cultivated within such containers.

[0011] These various known containers have multiple disadvantages, due to their formats and dimensions, their hermetic or non-hermetic closures, making their transport either difficult or under meticulous supervision, or impossible. In fact, non-hermetic containers require their maintenance in a controlled atmosphere.

[0012] In addition, they require transport with extreme precautions, making them impractical.

[0013] These containers also impose restrictions for introducing several solutions one after the other, without contamination.

[0014] STATEMENT OF THE INVENTION

[0015] The invention aims to overcome the drawbacks of the state of the art by proposing a closed device for culturing and transporting cell therapy products. Such a culture device has characteristics and various elements internally enabling the development of the cell culture in a protected environment, hermetically sealed by a lid or hermetically covered by a peelable film, while allowing the introduction and extraction of different solutions required for said culture, in a controlled manner and without risk of contamination.

[0016] In addition, the cultivation device allows for easy transportation of crops, without any restrictive precautions.

[0017] To do this, the cultivation device includes

[0018] - a container with a base topped with peripheral walls, delimiting an internal volume with an opening in the upper part;

[0019] - a closing means, attached in a removable attachment covering said opening; characterized in that

[0020] - at least one of the peripheral walls comprises at least two through channels opening into said internal volume.

[0021] According to additional, non-limiting characteristics, such a cultivation device may comprise

[0022] - two primary channels, arranged through a first of the peripheral walls;

[0023] - three secondary channels, arranged through a second of the peripheral walls.

[0024] According to one embodiment, said primary and secondary channels are equipped with plugs dedicated to: i) filtration allowing gas exchanges; j) an inlet allowing the introduction of a culture medium; jj) an inlet allowing the introduction of a hydrogel matrix of exclusively biological origin; jjj) an inlet allowing the introduction of cells; k) an outlet allowing the evacuation of said culture medium.

[0025] According to one embodiment, said device comprises

[0026] - a cutting tool sized and shaped for insertion into said internal volume until it comes into contact with said base;

[0027] - the cutting tool being provided on the lower face with at least one cutting module with beveled lower edges forming cutting blades.

[0028] According to one embodiment, said device comprises a pusher, attached to the upper face of said cutting tool.

[0029] According to one embodiment, said at least one cutting module has a generally rectangular parallelepiped shape, with a truncated corner.

[0030] According to one embodiment, said cutting tool comprises six cutting modules distributed in two rows and three columns.

[0031] According to one embodiment, said closing means is hermetic and comprises a cover, provided with lateral tabs for snap-fastening with said peripheral walls.

[0032] According to one embodiment, said closing means comprises a peelable cover attached in a hermetic manner by heat sealing on the upper edge of said peripheral walls.

[0033] The invention also relates to a kit for culturing and transporting a cell therapy product comprising

[0034] - a cultivation device according to the invention, characterized in that it comprises

[0035] - a sampling organ shaped like a shovel, sized to complement said cutting tool,

[0036] - said shovel comprising a handle connected to a flat end, provided with

[0037] - said flat end of said shovel comprises a rough surface.

[0038] PRESENTATION OF THE DRAWINGS

[0039] Other characteristics and advantages of the invention will emerge from the detailed description which follows of the non-limiting embodiments of the invention, with reference to the appended figures, in which:

[0040] [Fig. 1] schematically represents a perspective view of an embodiment of a cultivation kit, showing in particular said cultivation device with a pusher and two formats of cutting tools, as well as the cover and the scoop-shaped sampling member; [Fig. 2] schematically represents a perspective view of an embodiment of said cultivation device, closed by its cover and with five primary and secondary channels connected to different plugs;

[0041] [Fig. 3] schematically represents a view similar to Figure 2, showing the culture device hermetically sealed by a sealed film;

[0042] [Fig. 4] schematically represents a perspective view of several stacked culture devices;

[0043] [Fig. 5] schematically represents a perspective view from below of a format of the cutting tool with six cutting modules, showing in particular the beveled edges of each of said six cutting modules;

[0044] [Fig. 6] schematically represents a perspective view of an embodiment of the pusher, showing in particular an impact face provided with six cylindrical lugs;

[0045] [Fig. 7] schematically represents a view similar to figure 6, with said cultivation device, within the housing of which is introduced a cutting tool with six cutting modules.

[0046] DETAILED DESCRIPTION

[0047] The present invention falls within the medical field of tissue culture and relates to a device 1 for culturing and transporting cell therapy products, hereinafter referred to as “device”.

[0048] Such a device 1 is intended to be closed, namely that it has an internal volume accessible during certain operations, such as the collection of a cultured sample, but which can be closed, in order to define a closed environment, separated from the external atmosphere.

[0049] Further on, the device 1 comprises a container 2 with a bottom 20 surmounted by peripheral walls 21, delimiting an internal volume with an opening 22 in the upper part.

[0050] Such a container 2 may have any shape, preferably rectangular parallelepiped, in particular with rounded corners.

[0051] Furthermore, the container 2 has a generally flat bottom 20, in order to allow the development of the tissue culture.

[0052] According to a preferred embodiment, in order to facilitate storage and transport, several devices 1 can be stacked.

[0053] To do this, said peripheral walls 21 are shaped like an inverted U with a hollow. In other words, said peripheral walls 21 of a first device 1 have an internal space, open at the bottom, allowing the introduction and fitting of an upper part of the peripheral walls 21 of another device 1 thus fitted. In particular, said walls 21 comprise an upper edge forming an overhang 210 parallel or substantially parallel to said bottom 20. In short, the upper edge of the peripheral walls 21 is flat.

[0054] In addition, projecting relative to said overhang 210, said peripheral walls 21 comprise an offset 21 1 shaped complementary to said hollow, forming a stacking lug for several cultivation devices 1. In particular, said offset 21 1 is positioned set back relative to the external face of the peripheral walls 21, with a portion of the overhang 210 forming a bearing surface for the lower edge of the peripheral walls 21 of a superimposed device 1.

[0055] Furthermore, the peripheral walls 21 may project relative to the base 20, extending downwards, which makes it possible to nest several devices 1 in superposition, the container 2 of which is closed, in particular by a cover 30.

[0056] Such an offset 21 1 extends over all or part of the upper edge of the peripheral walls 21, preferably at least along two adjacent walls 21.

[0057] Said offset 21 1 may be continuous or interrupted, preferably continuous at the levels of at least two corners of said device 1.

[0058] According to a preferred embodiment, as visible in FIG. 3, said offset 21 1 extends along two adjacent peripheral walls 21 continuously, as well as over part of the length of the other two walls 21 , with rounded corners.

[0059] Furthermore, the offset 21 1 can serve as a key, for the purpose of vertical alignment according to the same orientation of at least two devices 1 thus nested.

[0060] Figure 4 shows three devices 1 superimposed and oriented in the same direction, in particular three stacked empty containers 2.

[0061] As mentioned previously, in order to determine a closed internal space suitable for tissue culture, the device 1 is designed to be closable and for this purpose comprises a closing means 3, attached in a removable attachment covering said opening 22.

[0062] According to an advantageous embodiment, said closing means 3 is provided to be hermetic and comprises a cover 30, provided with lateral tabs 31 for attachment by snap-fastening with said peripheral walls 21. For this purpose, the peripheral walls 21 may comprise any type of complementary member, shaped to cooperate by snap-fastening with said tabs 31. Therefore, in the snap-fastening position of the lateral tabs 31, the cover 30 ensures a total seal covering the opening 22, to air as well as to any fluid. In addition, the lower face of said cover 30 may comprise seals ensuring the seal in contact with the surface of the walls of the container 2 and by compression at the snap-fastening. As visible in FIG. 2, the snap-fastening of the tabs 31 can be carried out at the levels of the outer edges of the peripheral walls 21.

[0063] According to a preferred embodiment, as visible in Figures 1 and 2, the cover 30 comprises two tabs 31, arranged at two contiguous edges of said cover 30. The cover 30 is then positioned at the diametrically opposite corner in internal engagement with respect to the offset 21 1 made projecting with respect to the upper edges of said peripheral walls 21. The cover 30 is then, on the one hand, engaged against said offset 21 1 at two edges and, on the other hand, clipped by means of its tabs 31 cooperating by snap-fastening with the two opposite edges, ensuring its positioning and its maintenance in the closure of said opening 22.

[0064] Furthermore, said cover 30 may comprise on the lower face means ensuring sealing in the closed position, such as seals provided around the periphery of said lower face of said cover 30.

[0065] According to a preferred embodiment, said closure means 3 comprises a peelable cover 32 attached in a hermetic fixing by heat sealing on the upper edge of said peripheral walls 21. Such a cover 32 must ensure sealing against contaminants and fluids, coming from the outside as well as from the inside, while allowing the gas exchanges necessary for the proper development of the culture.

[0066] According to a preferred embodiment, the heat-sealing of said cover 32 is carried out on the overhang 21 1 provided on the upper edge of the peripheral walls 21.

[0067] Furthermore, said cover 32 can be made of any type of material, preferably of a composite or plastic material, such as a plastic from the polyethylene family. This material can be transparent or translucent, allowing light to pass through and making it possible to look inside the volume of the device 1, for example to monitor the development of the tissue culture. Such a material can also include a filtering coating, limiting or blocking certain wavelengths of light radiation, such as for example certain ultraviolet rays, which are harmful to the development of certain cells.

[0068] Figure 3 shows an example of a cover 32 in the form of a transparent plastic film, heat-sealed onto said overhang 21 1 . This embodiment shows in particular a part of the overhang 210 arranged internally relative to said offset 21 1 , forming a flat surface around the internal volume of the container 2.

[0069] According to a combined embodiment, the cover 30 can be attached over the cover 32. Advantageously, the invention allows the introduction and extraction of different solutions required for said culture, in a controlled manner and without risk of contamination, in particular by keeping the internal volume hermetically sealed.

[0070] To do this, the device 1 provides at least one of the peripheral walls 21 comprising at least two through channels 4 opening into said internal volume.

[0071] Said at least two channels 4 can be located at the level of the same peripheral wall 21, or at the levels of two different peripheral walls 21, preferably adjacent.

[0072] Each channel 4 comprises a portion projecting externally relative to the corresponding peripheral wall 21.

[0073] According to a preferred embodiment, the culture device 1 comprises two primary channels 40, 41, arranged through a first of the peripheral walls 21, as well as three secondary channels 42, 43, 44, arranged through a second of the peripheral walls 21.

[0074] Each of said primary channels 40,41 and secondary channels 42,43,44 are then dedicated to the introduction or extraction of a solution, as well as to gas exchanges.

[0075] According to one embodiment, said primary channels 40, 41 and said secondary channels 42, 43, 44 are equipped with plugs 400.

[0076] Such caps 400 are attached in removable fixation, in particular by fitting with said projecting portion of said channels 4 then forming a tip. Such a tip is preferably of the “LUER” or “LUER LOCK” type, international standard in the medical field, corresponding to a conicity of 6%, possibly with a traction-resistant notch, as well as a screwing system provided on said caps, allowing a connection with another medical device or organ.

[0077] Further, said plugs 400 are dedicated to: i) filtration allowing gas exchanges; j) an inlet allowing the introduction of a culture medium; jj) an inlet allowing the introduction of a hydrogel matrix of exclusively biological origin; jjj) an inlet allowing the introduction of cells; k) an outlet allowing the evacuation of said culture medium.

[0078] A device 1 equipped with five plugs 400 is visible in Figure 2.

[0079] According to one embodiment, said channels 4 can be connected to an automated or semi-automated culture system. Said caps 400 can therefore comprise connectors for tubes or tubing, or even a septum provided with a self-sealing membrane, generally made of silicone material (the material and thickness of this membrane allowing it to be pierced by a needle, and above all to retain its hermetic character once the needle is removed).

[0080] In particular, i) the filtration allowing exchanges can be equipped with a cap 400 provided with an elbow on which is mounted a filter, in particular at 0.22 pm, filtering particles and fluids, but allowing gas exchanges with the atmosphere; j) the inlet of the culture medium can be equipped with a cap 400 provided with a safety non-return valve, to prevent the medium from escaping to the outside once introduced through this channel 41, in particular during transport and handling; jj) the inlet of the matrix, such as the hydrogel), is used only once and includes a cap 400 which is intended to be sealed; jjj) the inlet of the cells, during seeding, is used only once and includes a cap 400 which is intended to be sealed; k) the outlet of the “consumed” medium includes a plug 400 equipped with a safety non-return valve, to prevent the medium from returning to the interior during its extraction.

[0081] Thus, during the use of the device 1 for tissue culture, first of all, after sterilization and closing of the container 2, in particular by the film 32, the matrix is ​​injected using a syringe, in particular of the standardized “LUER LOCK” type into the secondary channel 42. The air inside is purged by lightly pressing on the film 32, before closing said secondary channel 42 with a stopper 400. The matrix is ​​treated for a period of two hours (2h) at a temperature of 37.5°C (degrees Celsius), in particular within an incubator. Several devices 1 can then be covered by their lid 31 and stacked, for simultaneous incubation. This incubation allows in particular coagulation and adhesion to the bottom 20 of the container 2.

[0082] Then, a cell suspension is injected using a syringe, of the “LUER LOCK” type, through the secondary channel 43. The air is purged by lightly pressing on the film 32, before closing said channel 43 with a stopper 400.

[0083] Device 1 containing the matrix and the cell suspension is then treated for 24 hours at a temperature of 37.5°C, particularly in an incubator), to allow the cells to adhere to the matrix.

[0084] Then, periodically, every other day until reaching 7 days, the "consumed" medium contained in the device 1 is evacuated using a "LUER LOCK" type syringe via the secondary outlet channel 44. A spawning medium is injected using a "LUER LOCK" type syringe via the primary inlet channel 41.

[0085] Device 1 is returned to a temperature of 37.5°C, in particular within an incubator, until the next medium change. At the end, the “consumed” medium contained in device 1 is evacuated using a “LUER LOCK” type syringe via the secondary outlet channel 44. The fresh medium is injected using a “LUER LOCK” type syringe via the primary inlet channel 41. The filter is removed and channel 40 is sealed with a suitable cap 400, like channel 41.

[0086] The cover 31 is then clipped to the device 1, hermetically closing the assembly, protecting the cover 32: the device 1 is then ready to be transported.

[0087] According to one embodiment, the device 1 comprises a cutting tool 5 sized and shaped for its insertion within said internal volume until it comes into contact with said bottom 20. In other words, the tool 5 has smaller dimensions, in length and width, compared to the internal dimensions of the container 2, to allow its insertion and extraction inside said internal volume, until it comes into insertion against the surface of said bottom 20.

[0088] In addition, the cutting tool 5 is provided on the lower face with at least one cutting module 50 with beveled lower edges 51 forming cutting blades.

[0089] According to one embodiment, as visible in Figures 5 and 7, said cutting tool 5 comprises six cutting modules 50 distributed in two rows and three columns.

[0090] Thus, it is possible to divide the tissue culture into several portions or "grafts", in particular into as many grafts as there are 50 modules.

[0091] It will be noted that the bevel of the lower edges 51 of each cutting module 50 forms an inclination with the corresponding vertical wall. The inclination of this bevel can be oriented inwards or outwards.

[0092] Furthermore, the thickness of each edge 51 is minimal, forming a blade edge capable of penetrating and cutting through tissue culture.

[0093] Further, according to one embodiment, each cutting module 50 has a generally rectangular parallelepiped shape. Preferably, each cutting module 50 has a rectangular parallelepiped shape, with a truncated corner, used to cut a notch in each graft and determining a visually recognizable orientation of said graft, in particular to ensure that the keratinocytes are located superiorly during the manipulation of said graft, or to notice if it has turned over and to be able to put it back in the correct direction of the cutting tool 5 within the device 1. In particular, the truncated corner of a module 50 located at the periphery, close to an angle of the internal volume of the container 2, can then serve as a foolproofing device.

[0094] According to one embodiment, the device 1 comprises a pusher 6, attached to the upper face of said cutting tool 5. Such a pusher 6 serves as a surface to ensure, on the one hand, the gripping of said cutting tool 5 during its insertion and extraction within the container 2 and, on the other hand, as a support surface in a downward vertical direction in order to apply sufficient pressure to ensure the cutting of the tissue culture.

[0095] In particular, according to a preferred embodiment, the cutting can be carried out in jerks, by hammering the upper face of said pusher 6, for example using a surgical mallet.

[0096] To do this, said pusher 6 comprises on the upper face a striker, in the form of at least one impact contact face provided with solid lugs 61.

[0097] According to one embodiment, as seen in Figures 1 and 6, said lugs 61 have a cylindrical shape.

[0098] The knocker may comprise one or more lugs 61, preferably six lugs 61 distributed in two rows and three columns, corresponding to the embodiment with six cutting modules 50. The latter are then in vertical alignment with said six lugs 61 when said knocker is associated with said cutting tool 5, improving the pressure applied for cutting each of said modules 50.

[0099] In this respect, according to one embodiment, said pusher 6 can be removably attached to the cutting tool 5, in particular by complementary fitting.

[0100] According to the embodiment shown in Figure 1, the pusher 6 has a shoe 60, projecting on the lower face and dimensioned complementary, with the clearance close, to be introduced by sliding fitting into an imprint 52 with which the cutting tool 5 is provided on the upper face.

[0101] According to a preferred embodiment, said shoe 60 is provided with means for snap-fastening by clipping with said imprint 52, ensuring the locking of these two elements together.

[0102] Thus, the pusher 6 mounted with the cutting tool 5 form two integral elements, the lugs 6 of which ensure in particular resistance to impacts during hammering, as well as the integral transmission of the force thus applied, in order to ensure the cutting of the crop by the modules 50.

[0103] According to one embodiment, as visible in Figure 6, the lugs 61 can be consolidated by connections 62, improving the rigidity of said pusher 6. In addition, similar reinforcements can be provided on the internal face of said pusher 6.

[0104] It will be noted that the lugs 61 and the connections 62 can be produced integrally during the molding of the plastic part of said pusher 6.

[0105] According to an advantageous embodiment, the device 1 is made of plastic material. In other words, the various elements of said devices are made of plastic material, namely the container 2, the cover 30, or the cutting tool 5, or the pusher 6. Preferably, the device 1 is made of polypropylene (PP), in particular “HP671T” polypropylene.

[0106] According to one embodiment, said cutting tool 5 and / or the pusher 6 are made of composite or plastic material, ensuring increased resistance to impacts and providing a sufficiently strong and sharp blade edge for each cutting module 50.

[0107] According to one embodiment, all or part of the device 1 is made of PP having undergone a plasma-type treatment. Such a plasma treatment makes it possible to modify the Arithmetic Mean Roughness (Ra) of the surface of the device 1, in particular of the internal walls and especially of the bottom 20 of the container 2, increasing its hydrophilic properties, without generating particles or releasable substances. A plasma-treated PP plastic thus provides a surface suitable for coagulation and adhesion of fibrin.

[0108] Furthermore, the PP plastic material ensures that the characteristics of the device 1 are maintained during sterilization, particularly during sterilization by irradiation.

[0109] It should be noted that the PP plastic material also allows the sealing of the lid 32.

[0110] In addition, the PP plastic material provides impact resistance when cutting.

[0111] The invention also relates to a kit 7 for culturing and transporting a cell therapy product, comprising a culture device 1 according to the invention, as previously described.

[0112] An example of kit 7, with device 1 and its different parts, is notably visible in figure 1.

[0113] Advantageously, such a kit 7 comprises a sampling member 8 shaped in the form of a shovel, sized to complement said cutting tool 5.

[0114] According to one embodiment, said shovel comprises a handle 80 connected to a flat end 81. In particular, said flat end 81 has dimensions substantially equal to those of a module 50 of the cutting tool 5, preferably slightly larger dimensions, in order to ensure the removal of a previously cut graft, with sufficient clearance.

[0115] According to a preferred embodiment, said handle 80 is ridged, improving grip and preventing it from slipping in the hands.

[0116] According to an advantageous embodiment, said flat end 81 of the shovel comprises a rough surface, improving the adhesion of the graft taken and preventing it from slipping when it is taken.

[0117] According to a preferred embodiment, said flat end 81 comprises an inclined distal end, making it possible to scrape the bottom 20 and recover a graft. The flat end 81 also comprises two raised longitudinal edges, preventing the graft taken from sliding laterally during the removal or movement of said scoop. An example of such a removal member 8 in the form of a scoop is notably visible in FIG. 1.

[0118] According to one embodiment, said sampling member is entirely made of plastic material, preferably polypropylene (PP). Further, according to one embodiment, the device 1 has dimensions allowing the cultivation and transport of a tissue culture with a surface area of ​​approximately 60 square centimeters (cm 2 ), which can be divided when cutting into grafts of approximately 10 cm 2 , directly within device 1 without extracting said culture.

Claims

CLAIMS 1. Closed device (1) for culturing and transporting cell therapy products, comprising: - a container (2) with a bottom (20) surmounted by peripheral walls (21), delimiting an internal volume with an opening (22) in the upper part; - a closing means (3), attached in a removable manner covering said opening (22); characterized in that at least one of the peripheral walls (21) comprises at least two through channels (4) opening into said internal volume.

2. Cultivation device according to the preceding claim, characterized in that it comprises - two primary channels (40,41), arranged through a first of the peripheral walls (21); - three secondary channels (42,43,44), arranged through a second of the peripheral walls (21).

3. Culture device (1) according to the preceding claim, characterized in that said primary and secondary channels (40, 41, 42, 43, 44) are equipped with plugs dedicated to: i) filtration allowing gas exchanges; j) an inlet allowing the introduction of a culture medium; jj) an inlet allowing the introduction of a hydrogel matrix of exclusively biological origin; jjj) an inlet allowing the introduction of cells; k) an outlet allowing the evacuation of said culture medium.

4. Culture device (1) according to any one of the preceding claims, characterized in that it comprises - a cutting tool (5) sized and shaped for insertion into said internal volume until it comes into contact with said base (20); - the cutting tool (5) being provided on the lower face with at least one cutting module (50) with beveled lower edges (51) forming cutting blades.

5. Culture device (1) according to the preceding claim, characterized in that it comprises - a pusher (6), attached to the upper face of said cutting tool (5).

6. Cultivation device (1) according to one of claims 4 or 5, characterized in that - said at least one cutting module (50) has a generally rectangular parallelepiped shape, with a truncated corner.

7. Cultivation device (1) according to one of claims 4 to 6, characterized in that said cutting tool (5) comprises - six cutting modules (50) distributed in two rows and three columns.

8. Culture device (1) according to any one of the preceding claims, characterized in that - said closing means (3) is hermetic and comprises a cover (30), provided with lateral tabs (31) for snap-fastening with said peripheral walls (21).

9. Culture device (1) according to any one of the preceding claims, characterized in that - said closing means (3) comprises a peelable cover (32) attached in hermetic fixing by heat-sealing on the upper edge of said peripheral walls (21).

10. Kit (7) for culturing and transporting cell therapy product, comprising - a culture device (1) according to any one of claims 1 to 9, characterized in that said kit (7) comprises - a sampling member (8) shaped in the form of a shovel, sized complementary to said cutting tool - said shovel comprising a handle (80) connected to a flat end (81), provided with a rough surface.

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